OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Progress is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)

Article	Citations
From protein structure to an optimized chromatographic capture step using multiscale modeling	59
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	57
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	39
Issue Information	37
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	35
NS0 and CHO cell lines generate distinct quality profiles for non‐originator NISTmAbs	35
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	34
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	31
Machine learning‐assisted clone selection for intensified cell culture processes	30
A practical framework for clone selection, media‐feed screening, and upstream process parameter optimization for a mAb CHO cell culture process	29
Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process	29
A straightforward approach for converting existing batch production to an integrated continuous manufacturing suite by adopting membrane‐based technology	25
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	24
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	22
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	21
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	20
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	20
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	19
Current status of platelet manufacturing in 3D or bioreactors	18
Issue Information	18
	17
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	17
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	17
Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification ( HPCMP )	17
The production of succinate with more CO ₂ fixation reactions facilitate	17

Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	17
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	17
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	16
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	15
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	15
Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO	15
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	15
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	14
Development of virus‐like particles‐based vaccines against coronaviruses	14
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm	14
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Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	12
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach	12
The development of an automated microscope image tracking and analysis system	11
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2	11
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	10
Enhancing mRNA vaccine production: Optimization of in vitro transcription for improved yield and purity	10
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	10
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	10
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	10
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	10
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Shear stress and microRNAs for better metastatic cancer management	9
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	9
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	8
When will we have a clone? An industry perspective on the typical CLD timeline	8
Improving trans‐cinnamic acid production in a model cyanobacterium	8
General strategies for IgG‐like bispecific antibody purification	8
Issue Information	8
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
A size‐exclusion chromatography fingerprinting workflow for the development of flow‐through polishing operations for mAbs derived from continuous precipitation processes	8
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	8
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Modeling scalability of impurity precipitation in downstream biomanufacturing	7
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	7
Biotechnological potential of yeast cell wall: An overview	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	7

Optimization of carotenoid production by Umbelopsis ramanniana	7
Transmission of poly( dT ₆₀ )	7
Targeting Warburg effect in Chinese hamster ovary cell culture with pyruvate dehydrogenase kinase inhibitors	7
A case study showing the role of hydrophobicity variants and other enriched mAb proteoforms on filterability through a virus filter with productivity	7
	7
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	7
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	7
Issue Information	7
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
RETRACTION : Comparison of Expression Optimization of New Derivative of staphylokinase ( SAK ‐	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	6
Pharmaceutical proteins at the interfaces and the role of albumin	6
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	6
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Issue Information	6
Disulfide bond reduction of antibodies during clarification: A bench‐scale assay and a mitigation tool	6
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing	6
Issue Information	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	6
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	5
Issue Information	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Special issue on integrated continuous biomanufacturing	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes	5
Isoelectric point ( pI )‐based phase separation ( pI‐BPS ) purification of elastin‐like polypeptid	5
Scalable catalyst production process for oleate hydratase whole‐cell biocatalysis	5
Unveiling microbial and microalgal chassis for therapeutic and diagnostic protein expression	5
Single‐cell RNA sequencing reveals population‐based shifts in gene expression heterogeneity in an unstable, clonally derived	5
Triton CG ‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus ‐based	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Purification of antisense oligonucleotides using hydrophobic interaction chromatography	5
Multifaceted algae as an ingredient in alternative meat formulations	5
Application of fucosylation inhibitors for production of afucosylated antibody	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of	5
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK ‐92 cells in vitro	5
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	5
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	5
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Rapid development of a transferable R aman model using high‐throughput cell culture for monitoring monoclonal antibody titer	5
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence	5