OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Progress is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-11-01 to 2025-11-01.)

Article	Citations
From protein structure to an optimized chromatographic capture step using multiscale modeling	120
Issue Information	71
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	51
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	42
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	41
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	39
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	27
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	23
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	23
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	23
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	22
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	22
Issue Information	20
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	19
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	19
The production of succinate with more CO ₂ fixation reactions facilitate	19
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	18
Current status of platelet manufacturing in 3D or bioreactors	17
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	16
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	16
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	16
Development of virus‐like particles‐based vaccines against coronaviruses	15
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	15
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	15
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	15

Copper impurity of iron raw material contributes to improved cell culture performance	15
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	14
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	14
	14
Immobilized enzymes as potent antibiofilm agent	14
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	13
Issue Information	13
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	13
Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	13
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	13
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	12
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	12
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	12
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133 + by st	12
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	11
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	11
The development of an automated microscope image tracking and analysis system	11
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	10
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	10
Shear stress and microRNAs for better metastatic cancer management	10
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	10
Non‐thermal plasma decontamination of microbes: a state of the art	10
Improving trans‐cinnamic acid production in a model cyanobacterium	10
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	9
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	9
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
General strategies for IgG‐like bispecific antibody purification	9
When will we have a clone? An industry perspective on the typical CLD timeline	9
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	9
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	9
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Issue Information	8
Biotechnological potential of yeast cell wall: An overview	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	8
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7
Issue Information	7
	7
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Modeling scalability of impurity precipitation in downstream biomanufacturing	7
Transmission of poly( dT ₆₀ )	7

Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
Cation exchange as a single polishing step for conjugated peptides	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	6
Optimization of carotenoid production by Umbelopsis ramanniana	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Issue Information	6
Pharmaceutical proteins at the interfaces and the role of albumin	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	6
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	5
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
Issue Information	5
Isoelectric point ( pI )‐based phase separation ( pI‐BPS ) purification of elastin‐like polypeptid	5
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	5
Special issue on integrated continuous biomanufacturing	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from	5
Modification of C‐segment of Cfa DnaE split intein for improving	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Issue Information	5