OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Progress is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)

Article	Citations
Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule	89
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	66
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	61
Issue Information	46
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	32
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	31
Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank	27
Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures ofCHOcells co‐fed with glucose and lactic acid	26
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	25
From protein structure to an optimized chromatographic capture step using multiscale modeling	25
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	24
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	24
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	20
Issue Information	20
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	20
Characterization of Chinese hamster ovary cell culture feed media precipitate	18
The production of succinate with more CO₂ fixation reactions facilitated by RuBisCO‐based engineered Escherichia coli	18
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	18
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	17
Current status of platelet manufacturing in 3D or bioreactors	15
Process analytics 4.0: A paradigm shift in rapid analytics for biologics development	15
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	14
Copper impurity of iron raw material contributes to improved cell culture performance	13
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	13
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	13

Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	13
Issue Information	13
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	12
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Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	12
Immobilized enzymes as potent antibiofilm agent	12
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	12
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations	12
Improvement of the efficiency and quality in developing a new CHO host cell line	11
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	11
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	11
Retrospective assessment of clonal origin of cell lines	11
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	11
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	11
Development of virus‐like particles‐based vaccines against coronaviruses	11
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	10
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	10
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	10
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	10
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133+ by stimulation of Notch signaling pathway	10
The development of an automated microscope image tracking and analysis system	10
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	10
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	10
Surface decoration of low molecular weight polyethylenimine (LMW PEI) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
Hybrid fusion protein as a dual protease inhibitor for the healing of chronic wounds	9
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth	9
Prospective inference of bioprocess cell viability through chemometric modeling of fluorescence multiway data	9
General strategies for IgG‐like bispecific antibody purification	8
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	8
Comparison of multi‐column chromatography configurations through model‐based optimization	8
Bioengineered chimeric tRNA/pre‐miRNAs as prodrugs in cancer therapy	8
A cross‐linked anti‐TNF‐α aptamer for neutralization of TNF‐α‐induced cutaneous Shwartzman phenomenon: A simple and novel approach for improving aptamers' affinity and efficiency	8
Improving trans‐cinnamic acid production in a model cyanobacterium	8
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic production of alkanes	8
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system	8
When will we have a clone? An industry perspective on the typical CLD timeline	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
Transmission of poly(dT₆₀) single‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
Shear stress and microRNAs for better metastatic cancer management	8
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	7
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
Issue Information	7
Biotechnological potential of yeast cell wall: An overview	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	7
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7

A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	6
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	6
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Cation exchange as a single polishing step for conjugated peptides	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	6
Issue Information	6
Optimization of carotenoid production by Umbelopsis ramanniana	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Chemistry of lignin and hemicellulose structures interacts with hydrothermal pretreatment severity and affects cellulose conversion	6
PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	5
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Determination of protein concentration in downstream biomanufacturing processes by in‐line index of refraction	5
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	5
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	5
Issue Information	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Issue Information	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from Penicillium oxalicum	5
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	5
Modification of C‐segment of CfaDnaE split intein for improving clean‐in‐place in chromatography process	5
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	5
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	5