Biotechnology Progress

Article	Citations
Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule	89
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	66
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	61
Issue Information	46
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	32
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	31
Retrospective assessment of clonality of a legacy cell line by analytical subcloning of the master cell bank	27
Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures ofCHOcells co‐fed with glucose and lactic acid	26
From protein structure to an optimized chromatographic capture step using multiscale modeling	25
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	25
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	24
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	24
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	20
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	20
Issue Information	20
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	18
Characterization of Chinese hamster ovary cell culture feed media precipitate	18
The production of succinate with more CO2 fixation reactions facilitated by RuBisCO‐based engineered Escherichia coli	18
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	17
Process analytics 4.0: A paradigm shift in rapid analytics for biologics development	15
Current status of platelet manufacturing in 3D or bioreactors	15
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	14
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	13
Issue Information	13
Copper impurity of iron raw material contributes to improved cell culture performance	13

Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	13
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	13
Immobilized enzymes as potent antibiofilm agent	12
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	12
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations	12
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm medium	12
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Development and application of an analytical approach to assess an antibody's potential for disulfide reduction	12
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	11
Development of virus‐like particles‐based vaccines against coronaviruses	11
Improvement of the efficiency and quality in developing a new CHO host cell line	11
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2/0 hybridoma cell line	11
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	11
Retrospective assessment of clonal origin of cell lines	11
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	11
The development of an automated microscope image tracking and analysis system	10
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	10
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	10
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	10
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	10
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	10
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	10
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133+ by stimulation of Notch signaling pathway	10
Prospective inference of bioprocess cell viability through chemometric modeling of fluorescence multiway data	9
Surface decoration of low molecular weight polyethylenimine (LMW PEI) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
Hybrid fusion protein as a dual protease inhibitor for the healing of chronic wounds	9
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system	8
When will we have a clone? An industry perspective on the typical CLD timeline	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
Transmission of poly(dT60) single‐stranded DNA through polycarbonate track‐etched ultrafiltration membranes	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
Shear stress and microRNAs for better metastatic cancer management	8
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	8
General strategies for IgG‐like bispecific antibody purification	8
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	8
Comparison of multi‐column chromatography configurations through model‐based optimization	8
Bioengineered chimeric tRNA/pre‐miRNAs as prodrugs in cancer therapy	8
A cross‐linked anti‐TNF‐α aptamer for neutralization of TNF‐α‐induced cutaneous Shwartzman phenomenon: A simple and novel approach for improving aptamers' affinity and efficiency	8
Improving trans‐cinnamic acid production in a model cyanobacterium	8
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	8
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic production of alkanes	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	7
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
Modeling scalability of impurity precipitation in downstream biomanufacturing	7
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
Issue Information	7
Biotechnological potential of yeast cell wall: An overview	7

High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Chemistry of lignin and hemicellulose structures interacts with hydrothermal pretreatment severity and affects cellulose conversion	6
PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	6
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	6
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Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
Cation exchange as a single polishing step for conjugated peptides	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	6
Issue Information	6
Optimization of carotenoid production by Umbelopsis ramanniana	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	5
Modification of C‐segment of CfaDnaE split intein for improving clean‐in‐place in chromatography process	5
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	5
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	5
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	5
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Determination of protein concentration in downstream biomanufacturing processes by in‐line index of refraction	5
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	5
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	5
Issue Information	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Issue Information	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from Penicillium oxalicum	5
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK‐92 cells in vitro	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction byLimosilactobacillus reuteriDSM17938	4
Isoelectric point (pI)‐based phase separation (pI‐BPS) purification of elastin‐like polypeptides (ELPs) containing charged, biologically active fusion proteins (E	4
Chitosan based hybrid superabsorbent for controlled drug delivery application	4
The effect of biosynthesized selenium nanoparticles on the expression of CYP51A and HSP90 antifungal resistance genes in Aspergillus fumigatus and Aspergi	4
Triton CG‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus‐based gene therapy	4
Application of inline variable pathlength technology for rapid determination of dynamic binding capacity in downstream process development of biopharmaceuticals	4
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	4
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	4
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	4
Application of fucosylation inhibitors for production of afucosylated antibody	4
Hydrolysis of lignocellulose by anaerobic fungi produces free sugars and organic acids for two‐stage fine chemical production with Kluyveromyces marxianus	4
Prefiltration enhances performance of sterile filtration for glycoconjugate vaccines	4
Application of Ambr15 system for simulation of entire SARS‐CoV‐2 vaccine production process involving macrocarriers	4
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	4
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	4
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	4
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	4
A ribonucleoprotein‐based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering	4
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide	4
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence activated cell sorting	4
Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	3
Effect of iron addition on mAb productivity and oxidative stress in Chinese hamster ovary culture	3
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	3
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	3
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	3
Just passing through: Deploying aquaporins in microbial cell factories	3
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	3
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbubbles	3
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology‐ and methylation‐dependent manner	3
Issue Information	3
Model validation and selection in metabolic flux analysis and flux balance analysis	3
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	3
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of Haematococcus pluvialis	3
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	3
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	3
Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations	3
Modulation of high mannose levels in N‐linked glycosylation through cell culture process conditions to increase antibody‐dependent cell‐mediated cytotoxicity activity for an antibody biosimilar	3
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell concentrations	3
Cryopreservation practices in clinical and preclinical iPSC‐based cell therapies: Current challenges and future directions	3
Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes	3
Managing integrated continuous bioprocesses in real time: Deviations in product quality	3
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	2
Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on CHO cell culture performance as a tool to understanding heterogeneity eff	2

High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	2
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	2
Accelerated CMC workflows to enable speed to clinic in the COVID‐19 era: A multi‐company view from the biopharmaceutical industry	2
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	2
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	2
Issue Information	2
Identification and classification of host cell proteins during biopharmaceutical process development	2
Optimization of an artificial neural network topology using response surface methodology for microbial fuel cell power prediction	2
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	2
Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming	2
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	2
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Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	2
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	2
Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains	2
Optimization and feedback control system of dilution rate for 1,3‐propanediol in two‐stage fermentation: A theoretical study	2
Evaluation of lentiviral vector stability and development of ion exchange purification processes	2
An updated overview of the application of CAR‐T cell therapy in neurological diseases	2
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	2
Preventing pyruvate kinase muscle expression in Chinese hamster ovary cells curbs lactogenic behavior by altering glycolysis, gating pyruvate generation, and increasing pyruvate flux into t	2
Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	2
Self‐assembling protein nanocages for modular enzyme assembly by orthogonal bioconjugation	2
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	2
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	2
A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications	2
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeutics	2
Corrections to: Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide	2
Glass microspargers as effective frit spargers in single use bioreactors	2
Advances in polymer‐based cell encapsulation and its applications in tissue repair	2
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	2
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	2
Issue Information	2
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	2
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor supplementation	2
Rapid intensification of an established CHO cell fed‐batch process	2
Metabolic engineering of Yarrowia lipolytica for the production of isoprene	2
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	2
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	2
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	2
Issue Information	2
Implementation of mDoE‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	1
Antennapedia‐derived positively‐charged peptide faces multiple problems upon their usage as targeting ligand for liposomal doxorubicin	1
Issue Information	1
Effect of pH, NaCl concentration, and mAb concentration of feed solution on the filterability of Planova™ 20N and Planova™ BioEX	1
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	1
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	1
Demonstration of a robust high cell density transient CHO platform yielding mAb titers of up to 2 g/L without medium exchange	1
Gene copy number, gene configuration and LC/HC mRNA ratio impact on antibody productivity and product quality in targeted integration CHO cell lines	1
Issue Information	1
Impact of different trace elements on metabolic routes during heterotrophic growth of C. ljungdahlii investigated through online measurement of the carbon dioxide transfer rate	1
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	1
In silico optimization of a challenging bispecific antibody chromatography step	1
Dentin regeneration based on tooth tissue engineering: A review	1
Biosynthesis of 1,3‐propanodiol and 2,3‐butanodiol from residual glycerol in continuous cell‐immobilized Klebsiella pneumoniae bioreactors	1
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform (WuXiUPTM) in improved product quality and	1
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	1
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	1
Ionic liquid‐based in situ product removal design exemplified for an acetone–butanol–ethanol fermentation	1
Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	1
5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of PAX5	1
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	1
Understanding the effect of temperature downshift onCHOcell growth, antibody titer and product quality by intracellular metabolite profiling and in vivo monitoring of redox state	1
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	1
Use of Monte Carlo simulations for improved facility fit planning in downstream biomanufacturing and technology transfer	1
N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors	1
Rheology of enzyme liquefied corn stover slurries: The effect of solids concentration on yielding and flow behavior	1
“Just add small molecules” cell‐free protein synthesis: CombiningDNAtemplate and cell extract preparation into a single fermentation	1
Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines	1
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	1
A design space for the filtration of challenging monoclonal antibodies using Planova™ S20N, a new regenerated cellulose virus removal filter	1
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	1
Advanced strategies in glycosylation prediction and control during biopharmaceutical development: Avenues toward industry 4.0	1
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	1
Larvicidal potential of cell wall degrading enzymes from Trichoderma asperellum against Aedes aegypti (Diptera: Culicidae)	1
Issue Information	1
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	1
Application of a simple unstructured kinetic and cost of goods models to support T‐cell therapy manufacture	1
Analytical characterization of broadly neutralizing antibody CAP256LS heavy chain clipping during manufacturing development	1
Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	1
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	1
Smart process development: Application of machine‐learning and integrated process modeling for inclusion body purification processes	1
Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus	1
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and TAT peptide: In vitro and in vivo studies	1
Understanding adsorption behavior of Polysorbate‐20 to sterile filters in therapeutic proteins final filtration process	1
Assessment of shock pretreatment and alkali pretreatment on corn stover using enzymatic hydrolysis	1
Depth filter material process interaction in the harvest of mammalian cells	1
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	1
Tangential flow filtration facilitated fractionation of polymerized human serum albumin: Insights into the effects of molecular size on biophysical properties	1
A deep‐well plate enabled automated high‐throughput cell line development platform	1
Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments	1
The pandemic and resilience for the future: AccBio 2021	1
Preparation and characterization of poly (lactic‐co‐glycolic acid) nanofibers containing simvastatin coated with hyaluronic acid for using in periodontal tissue engineering	1