Biotechnology Progress

Article	Citations
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	82
From protein structure to an optimized chromatographic capture step using multiscale modeling	53
Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process	51
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	33
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	30
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	29
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	29
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	29
Issue Information	27
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	27
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	26
A practical framework for clone selection, media‐feed screening, and upstream process parameter optimization for a mAb CHO cell culture process	25
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	24
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	23
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	23
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	20
Issue Information	20
Current status of platelet manufacturing in 3D or bioreactors	19
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	19
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	19
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	19
The production of succinate with more CO 2 fixation reactions facilitate	18
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	17
Development of virus‐like particles‐based vaccines against coronaviruses	17
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Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	17
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	16
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	16
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	16
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm	15
Immobilized enzymes as potent antibiofilm agent	15
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO	15
Antibody glycation during a Chinese hamster ovary fed‐batch process is following a constrained second order reaction	14
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	13
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	13
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	13
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	13
The development of an automated microscope image tracking and analysis system	12
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2	12
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	11
Enhancing mRNA vaccine production: Optimization of in vitro transcription for improved yield and purity	11
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	11
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	10
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach	10
Improving trans‐cinnamic acid production in a model cyanobacterium	10
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	10
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Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	10
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	9
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	9
Shear stress and microRNAs for better metastatic cancer management	9
When will we have a clone? An industry perspective on the typical CLD timeline	9
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
General strategies for IgG‐like bispecific antibody purification	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
Issue Information	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	7
A size‐exclusion chromatography fingerprinting workflow for the development of flow‐through polishing operations for mAbs derived from continuous precipitation processes	7
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing	7
Transmission of poly( dT 60 )	7
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	7
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Issue Information	7
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	7
Biotechnological potential of yeast cell wall: An overview	7
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6

Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	6
RETRACTION : Comparison of Expression Optimization of New Derivative of staphylokinase ( SAK ‐	6
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	6
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	6
Disulfide bond reduction of antibodies during clarification: A bench‐scale assay and a mitigation tool	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Antibody charge variant modulation by in vitro enzymatic treatment in different Chinese hamster ovary cell cultures	6
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	6
A case study showing the role of hydrophobicity variants and other enriched mAb proteoforms on filterability through a virus filter with productivity	6
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Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Optimization of carotenoid production by Umbelopsis ramanniana	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Issue Information	6
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	5
Modification of C‐segment of Cfa DnaE split intein for improving	5
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	5
Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from	5
Issue Information	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Issue Information	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes	5
Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on	5
Special issue on integrated continuous biomanufacturing	5
Purification of antisense oligonucleotides using hydrophobic interaction chromatography	5
Triton CG ‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus ‐based	4
Application of fucosylation inhibitors for production of afucosylated antibody	4
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	4
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	4
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	4
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	4
Unveiling microbial and microalgal chassis for therapeutic and diagnostic protein expression	4
Managing integrated continuous bioprocesses in real time: Deviations in product quality	4
Rapid development of a transferable R aman model using high‐throughput cell culture for monitoring monoclonal antibody titer	4
Single‐cell RNA sequencing reveals population‐based shifts in gene expression heterogeneity in an unstable, clonally derived	4
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	4
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	4
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	4
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence	4
Just passing through: Deploying aquaporins in microbial cell factories	4
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	4
Application of Ambr15 system for simulation of entire SARS‐CoV ‐2 vaccine production process involving macrocarriers	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction by Limosilactobacillus reuteri DSM	4
Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions	4
Isoelectric point ( pI )‐based phase separation ( pI‐BPS ) purification of elastin‐like polypeptid	4
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	4
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	4
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	4
Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of	4
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK ‐92 cells in vitro	4
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	4
Evaluating the impact of ionic strength on retention of minute virus of mice during small virus‐retentive filtration	3
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	3
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	3
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	3
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Issue Information	3
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies	3
Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	3
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Issue Information	3
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	3
Glass microspargers as effective frit spargers in single use bioreactors	3
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell conce	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	3

Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	3
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3
Model validation and selection in metabolic flux analysis and flux balance analysis	3
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3
Cryopreservation practices in clinical and preclinical iPSC ‐based cell therapies: Current challenges and future directions	3
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbu	3
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	3
Issue Information	3
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Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	3
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	3
Accelerated CMC workflows to enable speed to clinic in the COVID ‐19 era: A multi‐company view fro	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeut	3
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	3
Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on	3
Optimizing monoclonal antibody biosimilar production via transfer and active learning for targeted quality profiles	3
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	3
Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies	3
An updated overview of the application of CAR‐T cell therapy in neurological diseases	3
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	3
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	3
Issue Information	3
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	3
Addressing challenging impurities in fusion proteins: A comprehensive review and cost‐effective strategy for HCP and low molecular weight species con	3
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	3
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	3
Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	3
A deep‐well plate enabled automated high‐throughput cell line development platform	2
Understanding adsorption behavior of Polysorbate‐20 to sterile filters in therapeutic proteins final filtration process	2
Depth filter material process interaction in the harvest of mammalian cells	2
Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines	2
Implementation of mDoE ‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	2
Secondary feed filtration and storage conditions influence trace element availability and process performance at 2000  L scale	2
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	2
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	2
Advanced strategies in glycosylation prediction and control during biopharmaceutical development: Avenues toward industry 4.0	2
Image analysis method for measurement and prediction of intra‐matrix IgG diffusion	2
Characterization of apoptosis in fed‐batch cultures highlights the impact of timely process intervention on improving recombinant protein productivity	2
Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	2
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	2
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor suppl	2
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	2
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	2
Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments	2
Correction to “Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in	2
A design space for the filtration of challenging monoclonal antibodies using Planova™ S20N , a new regenerated cellulose virus removal filter	2
Analytical characterization of broadly neutralizing antibody CAP256LS heavy chain clipping during manufacturing development	2
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	2
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform ( WuXiUP	2
Understanding the effect of temperature downshift on CHO cell growth, antibody titer and product quality by intracellular metabolite profiling and in	2
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	2
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	2
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	2
N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors	2
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	2
Evaluation of lentiviral vector stability and development of ion exchange purification processes	2
Scalable desolvation synthesis, storage stability, and comprehensive biophysical characterization of human hemoglobin nanoparticles as a potential hemoglobin‐based oxygen carrier	2
Issue Information	2
5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of	2
Use of live digital twin (shadow) soft sensor to monitor membrane degradation in continuous manufacturing single pass tangential flow filtration	2
Effect of pH, NaCl concentration, and mAb concentration of feed solution on the filterability of Planova™ 20N and Planova™ BioEX	2
Dentin regeneration based on tooth tissue engineering: A review	2
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and T	2
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	2
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	2
Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	2
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	2
A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy	2
“Just add small molecules” cell‐free protein synthesis: Combining DNA template and cell extract preparation into a single fermentation	2
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	2
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Issue Information	2