Biotechnology Progress

Article	Citations
Lignin degradation by microorganisms: A review	60
Developments of biobased plasticizers for compostable polymers in the green packaging applications: A review	47
A novel pH‐responsive nanoniosomal emulsion for sustained release of curcumin from a chitosan‐based nanocarrier: Emphasis on the concurrent improvement of loading, sustained release, and ap	44
Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat	32
Computational drug repurposing study of the RNA binding domain of SARS‐CoV‐2 nucleocapsid protein with antiviral agents	30
In vitro hepatic steatosis model based on gut–liver‐on‐a‐chip	29
Green and sustainable route for oxidative depolymerization of lignin: New platform for fine chemicals and fuels	27
Economic assessment of continuous processing for manufacturing of biotherapeutics	27
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	23
Reshaping cell line development and CMC strategy for fast responses to pandemic outbreak	23
Identification and characterization of a residual host cell protein hexosaminidase B associated with N‐glycan degradation during the stability study of a therapeutic recombinant monoclonal anti	23
Synthesis of a novel nanocomposite containing chitosan as a three‐dimensional printed wound dressing technique: Emphasis on gene expression	22
Deep brain stimulation: Challenges at the tissue‐electrode interface and current solutions	21
Advances in 3D printing of composite scaffolds for the repairment of bone tissue associated defects	21
The effect of biosynthesized selenium nanoparticles on the expression of CYP51A and HSP90 antifungal resistance genes in Aspergillus fumigatus and Aspergi	21
Process and operations strategies to enable global access to antibody therapies	20
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	19
Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase‐mediated cassette exchange	19
Raman based chemometric model development for glycation and glycosylation real time monitoring in a manufacturing scale CHO cell bioreactor process	18
Anti‐epithelial cell adhesion molecule RNA aptamer‐conjugated liposomal doxorubicin as an efficient targeted therapy in mice bearing colon carcinoma tumor model	18
In silico process characterization for biopharmaceutical development following the quality by design concept	18
Using microalgae for remediation of crude petroleum oil–water emulsions	18
Devices and techniques used to obtain and analyze three‐dimensional cell cultures	17
Nanoparticles in cancer immunotherapies: An innovative strategy	16
Rapid intensification of an established CHO cell fed‐batch process	15

Synthetic biology in biofilms: Tools, challenges, and opportunities	14
Immobilized enzymes as potent antibiofilm agent	14
Adapting virus filtration to enable intensified and continuous monoclonal antibody processing	14
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO/ZnO nanoparticles for superficial wound healing: In vitro and in vivo evaluations	13
Chemistry of lignin and hemicellulose structures interacts with hydrothermal pretreatment severity and affects cellulose conversion	13
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	13
Immunoliposomes bearing lymphocyte activation gene 3 fusion protein and P5 peptide: A novel vaccine for breast cancer	13
Differential responses in EPA and fucoxanthin production by the marine diatom Stauroneis sp. under varying cultivation conditions	13
Metabolic trends of Chinese hamster ovary cells in biopharmaceutical production under batch and fed‐batch conditions	13
Pumpless, unidirectional microphysiological system for testing metabolism‐dependent chemotherapeutic toxicity	12
Hydrolysis of lignocellulose by anaerobic fungi produces free sugars and organic acids for two‐stage fine chemical production with Kluyveromyces marxianus	12
PVA based nanofiber containing cellulose modified with graphitic carbon nitride/nettles/trachyspermum accelerates wound healing	12
Metabolic engineering of Yarrowia lipolytica for the production of isoprene	12
High‐throughput and automation advances for accelerating single‐cell cloning, monoclonality and early phase clone screening steps in mammalian cell line development for biologics production	11
Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid	11
Induced pluripotent stem cells can utilize lactate as a metabolic substrate to support proliferation	11
Metabolic profiling of Chinese hamster ovary cell cultures at different working volumes and agitation speeds using spin tube reactors	11
Model‐based workflow for scale‐up of process strategies developed in miniaturized bioreactor systems	11
Effects of variable domain orientation on anti‐HER2 single‐chain variable fragment antibody expressed in the Escherichia coli cytoplasm	11
Yeast cells for encapsulation of bioactive compounds in food products: A review	11
The function of chitosan/agarose biopolymer on Fe2O3 nanoparticles and evaluation of their effects on MCF‐7 breast cancer cell line and expression of B	10
A rapid novel strategy for screening of antibody phage libraries for production, purification, and functional characterization of amber stop codons containing single‐chain antibody fragments	10
Ionic liquid‐based in situ product removal design exemplified for an acetone–butanol–ethanol fermentation	10
Development of virus‐like particles‐based vaccines against coronaviruses	10
Hypoxia preconditioned mesenchymal stem cell‐derived exosomes induce ex vivo expansion of umbilical cord blood hematopoietic stem cells CD133+ by stimulation of Notch signaling pathway	10
Molecularly imprinted spongy columns for Angiotensin(II) recognition from human serum	10
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and TAT peptide: In vitro and in vivo studies	10
CHOK1SV GS‐KO SSIexpression system: A combination of theFer1L4locus and glutamine synthetase selection	9
Immobilization of urease enzyme on chitosan/polyvinyl alcohol electrospun nanofibers	9
Bax and Bak knockout apoptosis‐resistant Chinese hamster ovary cell lines significantly improve culture viability and titer in intensified fed‐batch culture process	9
α‐Acetolactate decarboxylase immobilized in chitosan: A highly stable biocatalyst to prevent off‐flavor in beer	9
Development of a miniature bioreactor model to study the impact of pH and DOT fluctuations on CHO cell culture performance as a tool to understanding heterogeneity eff	9
A mechanistic model to account for the Donnan and volume exclusion effects in ultrafiltration/diafiltration process of protein formulations	9
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	9
Microfluidic chip‐based single‐cell cloning to accelerate biologic production timelines	8
Transfer of continuous manufacturing process principles for mAb production in a GMP environment: A step in the transition from batch to continuous	8
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeutics	8
Characterization of Chinese hamster ovary cell culture feed media precipitate	8
Production of biodiesel by Burkholderia cepacia lipase as a function of process parameters	8
Biophysical analysis of SARS‐CoV‐2 transmission and theranostic development via N protein computational characterization	8
Larvicidal potential of cell wall degrading enzymes from Trichoderma asperellum against Aedes aegypti (Diptera: Culicidae)	8
X‐ray sterilization of biopharmaceutical manufacturing equipment—Extractables profile of a film material and copolyester Tritan™ compared to gamma irradiation	8
Evaluation of lentiviral vector stability and development of ion exchange purification processes	8
Rheology of enzyme liquefied corn stover slurries: The effect of solids concentration on yielding and flow behavior	8
Transferability of miRNA‐technology to bioprocessing: Influence of cultivation mode and media	8
The pandemic and resilience for the future: AccBio 2021	8
High‐capacity multimodal anion‐exchange membranes for polishing of therapeutic proteins	7
Effect of filtrate flux and process disruptions on virus retention by a relatively homogeneous virus removal membrane	7
Accelerated CMC workflows to enable speed to clinic in the COVID‐19 era: A multi‐company view from the biopharmaceutical industry	7
Smart process development: Application of machine‐learning and integrated process modeling for inclusion body purification processes	7

Analysis of fouling and breakthrough of process related impurities during depth filtration using confocal microscopy	7
Current challenges and recent advances on the path towards continuous biomanufacturing	7
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	7
Biophysical properties of tense quaternary state polymerized human hemoglobins bracketed between 500 kDa and 0.2 μm in size	7
Safety risk management for low molecular weight process‐related impurities in monoclonal antibody therapeutics: Categorization, risk assessment, testing strategy, and process development with leveragi	7
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	7
Improvement of the efficiency and quality in developing a new CHO host cell line	7
Chromatographic capture of cells to achieve single stage clarification in recombinant protein purification	7
Optimization and feedback control system of dilution rate for 1,3‐propanediol in two‐stage fermentation: A theoretical study	7
Prebiotic peptides, their formation, fermentation in the gut, and health implications	7
Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes	7
Polyunsaturated fatty acids production by solid‐state fermentation on polyurethane foam by Mortierella alpina	6
The impact of temozolomide and lonafarnib on the stemness marker expression of glioblastoma cells in multicellular spheroids	6
Identification and classification of host cell proteins during biopharmaceutical process development	6
Self‐assembling protein nanocages for modular enzyme assembly by orthogonal bioconjugation	6
Evaluation of the efficiency of modified PAMAM dendrimer with low molecular weight protamine peptide to deliver IL ‐12 plasmid int	6
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	6
Effect of nitrate feeding strategies on lipid and biomass productivities in fed‐batch cultures of Nannochloropsis gaditana	6
Impact of X‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	6
Applications of flow cytometry sorting in the pharmaceutical industry: A review	6
Identification of compendial nonionic detergents for the replacement of Triton X‐100 in bioprocessing	6
Effect of iron addition on mAb productivity and oxidative stress in Chinese hamster ovary culture	6
Microbioreactor (micro‐Matrix) potential in aerobic and anaerobic conditions with different industrially relevant microbial strains	6
Structure and binding ability of self‐assembled α‐lactalbumin protein nanotubular gels	6
Modeling flux in tangential flow filtration using a reverse asymmetric membrane for Chinese hamster ovary cell clarification	6
Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii	6
A novel NCI‐H69V small cell lung cancer functional mini‐tumor model for future treatment screening applications	5
Application of mesenchymal stem cells in regenerative medicine: A new approach in modern medical science	5
Determination of protein concentration in downstream biomanufacturing processes by in‐line index of refraction	5
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	5
Dentin regeneration based on tooth tissue engineering: A review	5
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	5
Effect of particle size distribution on filtration performance of cell culture medium	5
Transgenic plants expressing a Clostridium difficile spore antigen as an approach to develop low‐cost oral vaccines	5
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Probing morphological, genetic and metabolomic changes of in vitro embryo development in a microfluidic device	5
Modulation of high mannose levels in N‐linked glycosylation through cell culture process conditions to increase antibody‐dependent cell‐mediated cytotoxicity activity for an antibody biosimilar	5
Scale‐up issues during sterile filtration of glycoconjugate vaccines	5
Application of Ambr15 system for simulation of entire SARS‐CoV‐2 vaccine production process involving macrocarriers	5
Control strategy for biopharmaceutical production by model predictive control	5
Impact of different trace elements on metabolic routes during heterotrophic growth of C. ljungdahlii investigated through online measurement of the carbon dioxide transfer rate	5
Preparation and characterization of poly (lactic‐co‐glycolic acid) nanofibers containing simvastatin coated with hyaluronic acid for using in periodontal tissue engineering	5
Impact of iron raw materials and their impurities onCHOmetabolism and recombinant protein product quality	5
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	5
Exometabolome profiling reveals activation of the carnitine buffering pathway in fed‐batch cultures ofCHOcells co‐fed with glucose and lactic acid	5
Bio‐based composite granules with simultaneous biocontrol and phosphorus fertilization roles: Outcomes from a lab‐scale in vitro assessment	5
Quantification of glycated IgG in CHO supernatants: A practical approach	5
Targeted co‐delivery of paclitaxel and anti P‐gp shRNA by low molecular weight PEI decorated with L‐3,4‐dihydroxyphenylalanine	4
Optimized prodigiosin production with Pseudomonas putida KT2440 using parallelized noninvasive online monitoring	4
A quantitative model for metabolic intervention using gut microbes	4
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	4
Application of machine learning methods to pathogen safety evaluation in biological manufacturing processes	4
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	4
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	4
Characterization of soy protein hydrolysates and influence of its iron content on monoclonal antibody production by a murine hybridoma cell line	4
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	4
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF‐18 peptide	4
Optimization of the nutritional environment for differentiation of human‐induced pluripotent stem cells using design of experiments—A proof of concept	4
Efficient enzymatic synthesis ofL‐ascorbyl palmitate usingCandida antarcticalipase B‐embedded metal‐organic framework	4
Preventing pyruvate kinase muscle expression in Chinese hamster ovary cells curbs lactogenic behavior by altering glycolysis, gating pyruvate generation, and increasing pyruvate flux into t	4
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	4
Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule	4
An assessment of the impact of Raman based glucose feedback control on CHO cell bioreactor process development	4
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	4
Biosynthesis of 1,3‐propanodiol and 2,3‐butanodiol from residual glycerol in continuous cell‐immobilized Klebsiella pneumoniae bioreactors	4
Prefiltration enhances performance of sterile filtration for glycoconjugate vaccines	4
Use of scanning electron microscopy and energy dispersive X‐ray spectroscopy to identify key fouling species during alternating tangential filtration	4
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	4
Toward optimal clearance: A universal affinity‐based mass spectrometry approach for comprehensive ELISA reagent coverage evaluation and HCP hitchhiker analysis	4
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	4
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
A Novel Polyculture Growth Model of Native Microalgal Communities to Estimate Biomass Productivity for Biofuel Production	3
Tuning metabolic efficiency for increased product yield in high titer fed‐batch Chinese hamster ovary cell culture	3
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	3
Isoelectric point (pI)‐based phase separation (pI‐BPS) purification of elastin‐like polypeptides (ELPs) containing charged, biologically active fusion proteins (E	3
A cross‐linked anti‐TNF‐α aptamer for neutralization of TNF‐α‐induced cutaneous Shwartzman phenomenon: A simple and novel approach for improving aptamers' affinity and efficiency	3
A ribonucleoprotein‐based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering	3
One‐step production of fully biotinylated and glycosylated human Fc gamma receptors	3
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3

Process analytics 4.0: A paradigm shift in rapid analytics for biologics development	3
Assessment of shock pretreatment and alkali pretreatment on corn stover using enzymatic hydrolysis	3
Recent advances of dendrimer in targeted delivery of drugs and genes to stem cells as cellular vehicles	3
Particle‐based analysis elucidates the real retention capacities of virus filters and enables optimal virus clearance study design with evaluation systems of diverse virological characteristics	3
Characterization of cell cycle and apoptosis in Chinese hamster ovary cell culture using flow cytometry for bioprocess monitoring	3
Experimental and computational characterization of mass transfer in high turndown bioreactors	3
Chitosan based hybrid superabsorbent for controlled drug delivery application	3
Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	3
An innovative strategy to recycle permeate in biologics continuous manufacturing process to improve material efficiency and sustainability	3
A new strategy for the decellularization of whole organs by hydrostatic pressure	3
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	3
Preparation of regenerable magnetic nanoparticles for cellulase immobilization: Improvement of enzymatic activity and stability	3
Considerations for operational space definition and optimization of a no‐salt flowthrough hydrophobic interaction chromatography purification step	3
Generation of a host cell line containing a MAR‐rich landing pad for site‐specific integration and expression of transgenes	3
Interaction of leachable model compounds and their impact on Chinese hamster ovary cell cultivation	3
Economic simulation of batch and continuous aqueous two‐phase purification for viral products	3
Optimization of an artificial neural network topology using response surface methodology for microbial fuel cell power prediction	3
Review: High temperature short time treatment of cell culture media and feed solutions to mitigate adventitious viral contamination in the biopharmaceutical industry	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
Antibiofilm effect of mono‐rhamnolipids and di‐rhamnolipids on carbon steel submitted to oil produced water	2
Establishment and characterization of lung co‐culture spheroids for paclitaxel loaded Eudragit® RL 100 nanoparticle evaluation	2
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	2
Gene copy number, gene configuration and LC/HC mRNA ratio impact on antibody productivity and product quality in targeted integration CHO cell lines	2
Glass microspargers as effective frit spargers in single use bioreactors	2
Protein adsorption on core‐shell resins for flow‐through purifications: Effect of protein molecular size, shape, and salt concentration	2
Integrated evaluation the antioxidant activity of epicatechin from cell dynamics	2
Advances in polymer‐based cell encapsulation and its applications in tissue repair	2
Evaluation of single‐use tangential flow filtration technology for purification of activated polysaccharides used in conjugate vaccine manufacturing	2
Understanding the effect of temperature downshift onCHOcell growth, antibody titer and product quality by intracellular metabolite profiling and in vivo monitoring of redox state	2
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	2
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	2
Development of an activated carbon filtration step and high throughput screening method to remove host cell proteins from a recombinant enzyme process	2
Rapid serum‐free /suspension adaptation: Medium development using a definitive screening design for Chinese hamster ovary cells	2
Purification of Cas9—RNA complexes by ultrafiltration	2
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	2
Valorizing prickly pear cladodes via methane‐arrested anaerobic digestion for carboxylic acid production	2
Demonstration of a robust high cell density transient CHO platform yielding mAb titers of up to 2 g/L without medium exchange	2
Modulating and optimizing Pluronic F‐68 concentrations and feeding for intensified perfusion Chinese hamster ovary cell cultures	2
A TGF‐α and TGF‐β1 Poloxamer‐based micelle/hydrogel composite: A promising novel candidate for the treatment of anosmia	2
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	2
Rapid metal speciation of cell culture media using reversed‐phase separations and inductively coupled plasma optical emission spectrometry	2
Copper impurity of iron raw material contributes to improved cell culture performance	2
Utilizing targeted integration CHO pools to potentially accelerate the GMP manufacturing of monoclonal and bispecific antibodies	2
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	2
Consolidated plasmid Design for Stabilized Heterologous Production of the complex natural product Siderophore Yersiniabactin	2
Expressing antigen binding fragments with high titers in a targeted integration CHO host by optimizing expression vector gene copy numbers and position: A case study	2
Model validation and selection in metabolic flux analysis and flux balance analysis	2
Streamlining process characterization efforts using the high throughput ambr® crossflow system for ultrafiltration and diafiltration processing of monoclonal antibodies	2
Understanding the interaction of proteins to ion exchange chromatographic supports: A surface energetics approach	2
Optimization and characterization of rhamnolipid production by Pseudomonas aeruginosa NY3 using waste frying oil as	2
When will we have a clone? An industry perspective on the typical CLD timeline	2
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	2
Efficient immobilized phospholipase A1 on Mo‐basing nanomaterials for enzymatic degumming	2
Single‐stage chromatographic clarification of Chinese Hamster Ovary cell harvest reduces cost of protein production	2
Optimization of carotenoid production by Umbelopsis ramanniana	2
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	2
A novel modified culture medium for enhancing redifferentiation of chondrocytes for cartilage tissue engineering applications	2
Analytical characterization of broadly neutralizing antibody CAP256LS heavy chain clipping during manufacturing development	2
CHOsynthetic promoters improve expression and product quality of biotherapeutic proteins	2
“Just add small molecules” cell‐free protein synthesis: CombiningDNAtemplate and cell extract preparation into a single fermentation	2
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	2
Quantifying the hydrodynamic stress for bioprocesses	2
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducib	2
Surface decoration of low molecular weight polyethylenimine (LMW PEI) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	2