Biotechnology Progress

Article	Citations
From protein structure to an optimized chromatographic capture step using multiscale modeling	59
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	57
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	39
Issue Information	37
NS0 and CHO cell lines generate distinct quality profiles for non‐originator NISTmAbs	35
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	35
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	34
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	31
Machine learning‐assisted clone selection for intensified cell culture processes	30
A practical framework for clone selection, media‐feed screening, and upstream process parameter optimization for a mAb CHO cell culture process	29
Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process	29
A straightforward approach for converting existing batch production to an integrated continuous manufacturing suite by adopting membrane‐based technology	25
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	24
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	22
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	21
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	20
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	20
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	19
Issue Information	18
Current status of platelet manufacturing in 3D or bioreactors	18
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	17
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	17
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Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	17
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	17

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification ( HPCMP )	17
The production of succinate with more CO 2 fixation reactions facilitate	17
Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	16
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	15
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	15
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	15
Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	15
Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO	15
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm	14
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	14
Development of virus‐like particles‐based vaccines against coronaviruses	14
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Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	12
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach	12
The development of an automated microscope image tracking and analysis system	11
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2	11
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	10
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	10
Enhancing mRNA vaccine production: Optimization of in vitro transcription for improved yield and purity	10
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	10
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	10
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	10
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	10
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	9
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	9
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Shear stress and microRNAs for better metastatic cancer management	9
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	9
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
A size‐exclusion chromatography fingerprinting workflow for the development of flow‐through polishing operations for mAbs derived from continuous precipitation processes	8
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	8
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	8
Non‐thermal plasma decontamination of microbes: a state of the art	8
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	8
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	8
When will we have a clone? An industry perspective on the typical CLD timeline	8
Improving trans‐cinnamic acid production in a model cyanobacterium	8
General strategies for IgG‐like bispecific antibody purification	8
Issue Information	8
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	7
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	7
Issue Information	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	7
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7
Modeling scalability of impurity precipitation in downstream biomanufacturing	7

Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	7
Biotechnological potential of yeast cell wall: An overview	7
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	7
Optimization of carotenoid production by Umbelopsis ramanniana	7
Transmission of poly( dT 60 )	7
Targeting Warburg effect in Chinese hamster ovary cell culture with pyruvate dehydrogenase kinase inhibitors	7
A case study showing the role of hydrophobicity variants and other enriched mAb proteoforms on filterability through a virus filter with productivity	7
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A novel synthetic sRNA promoting protein overexpression in cell‐free systems	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing	6
Issue Information	6
Pharmaceutical proteins at the interfaces and the role of albumin	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
RETRACTION : Comparison of Expression Optimization of New Derivative of staphylokinase ( SAK ‐	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Issue Information	6
Disulfide bond reduction of antibodies during clarification: A bench‐scale assay and a mitigation tool	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	6
Single‐cell RNA sequencing reveals population‐based shifts in gene expression heterogeneity in an unstable, clonally derived	5
Triton CG ‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus ‐based	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Purification of antisense oligonucleotides using hydrophobic interaction chromatography	5
Multifaceted algae as an ingredient in alternative meat formulations	5
Application of fucosylation inhibitors for production of afucosylated antibody	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of	5
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK ‐92 cells in vitro	5
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	5
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	5
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Rapid development of a transferable R aman model using high‐throughput cell culture for monitoring monoclonal antibody titer	5
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence	5
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	5
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	5
Issue Information	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Special issue on integrated continuous biomanufacturing	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes	5
Isoelectric point ( pI )‐based phase separation ( pI‐BPS ) purification of elastin‐like polypeptid	5
Scalable catalyst production process for oleate hydratase whole‐cell biocatalysis	5
Unveiling microbial and microalgal chassis for therapeutic and diagnostic protein expression	5
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	4
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies	4
Data‐driven design of deterministic lateral displacement microfluidic devices for CTC separation	4
Optimizing monoclonal antibody biosimilar production via transfer and active learning for targeted quality profiles	4
Issue Information	4
Managing integrated continuous bioprocesses in real time: Deviations in product quality	4
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	4
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	4
Evaluating the impact of ionic strength on retention of minute virus of mice during small virus‐retentive filtration	4
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	4
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	4
Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	4
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell conce	4
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Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction by Limosilactobacillus reuteri DSM	4
Model validation and selection in metabolic flux analysis and flux balance analysis	4
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	4
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	4
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	4
A biochemically structured predictive model of eukaryotic microalgal growth: Integrating radiative transfer, respiration, and pigment acclimation	4

Generation of high‐expressing transposon‐based stable pools to produce (1 + 1) and (2 + 1) bispecific common light chain antibodies	4
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	4
Just passing through: Deploying aquaporins in microbial cell factories	4
Cryopreservation practices in clinical and preclinical iPSC ‐based cell therapies: Current challenges and future directions	4
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	4
Addressing challenging impurities in fusion proteins: A comprehensive review and cost‐effective strategy for HCP and low molecular weight species con	4
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	3
Image analysis method for measurement and prediction of intra‐matrix IgG diffusion	3
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform ( WuXiUP	3
Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	3
Endotoxin removal by virus filters	3
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	3
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeut	3
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	3
Issue Information	3
High‐throughput development and scale‐up of recombinant adeno‐associated virus 5 anion‐exchange chromatography for high‐purity capsid separation	3
Cover	3
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	3
Issue Information	3
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	3
Implementation of mDoE ‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	3
Issue Information	3
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	3
Secondary feed filtration and storage conditions influence trace element availability and process performance at 2000  L scale	3
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	3
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	3
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	3
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Glass microspargers as effective frit spargers in single use bioreactors	3
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor suppl	3
An updated overview of the application of CAR‐T cell therapy in neurological diseases	3
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	3
Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
Accelerated CMC workflows to enable speed to clinic in the COVID ‐19 era: A multi‐company view fro	3
Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	3
Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	3
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	3
A mechanistic model for recombinant adeno‐associated virus production dynamics in a helper virus‐assisted producer cell line	3
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	3
Issue Information	3
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	3
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	3
Evaluation of lentiviral vector stability and development of ion exchange purification processes	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	3
Issue Information	3
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A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy	3
Functional CRISPR ‐ Cas9 knockout screening of the genetic determinants of human fibroblast migrat	2
Tangential flow filtration facilitated fractionation of polymerized human serum albumin: Insights into the effects of molecular size on biophysical properties	2
A design space for the filtration of challenging monoclonal antibodies using Planova™ S20N , a new regenerated cellulose virus removal filter	2
Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments	2
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	2
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	2
Characterization of apoptosis in fed‐batch cultures highlights the impact of timely process intervention on improving recombinant protein productivity	2
“Just add small molecules” cell‐free protein synthesis: Combining DNA template and cell extract preparation into a single fermentation	2
Correction to “Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in	2
Harvesting AAV by tangential flow filtration using reverse asymmetric membranes	2
A TGF ‐α and TGF ‐β1 Poloxamer‐based micelle/hydrogel composite: A promising novel candidate for t	2
Use of Monte Carlo simulations for improved facility fit planning in downstream biomanufacturing and technology transfer	2
Use of live digital twin (shadow) soft sensor to monitor membrane degradation in continuous manufacturing single pass tangential flow filtration	2
In silico optimization of a challenging bispecific antibody chromatography step	2
Depth filter material process interaction in the harvest of mammalian cells	2
5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of	2
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	2
Scalable desolvation synthesis, storage stability, and comprehensive biophysical characterization of human hemoglobin nanoparticles as a potential hemoglobin‐based oxygen carrier	2
A deep‐well plate enabled automated high‐throughput cell line development platform	2
Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines	2
Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus	2
Effect of pH, NaCl concentration, and mAb concentration of feed solution on the filterability of Planova™ 20N and Planova™ BioEX	2
Microcontact printing of lectin self‐assembled monolayers for arbovirus detection	2
Multimodal peptide ligand extracts parvovirus from interface in affinity aqueous two‐phase system	2
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	2
Understanding adsorption behavior of Polysorbate‐20 to sterile filters in therapeutic proteins final filtration process	2
A PVA /chitosan/gelatin scaffold incorporating salicylic acid‐loaded TiO ₂ nanoparticles enhances	2
Augmenting therapeutic protein production in CHO cells: A proline‐based selection strategy for enhanced productivity and product quality	2
Dentin regeneration based on tooth tissue engineering: A review	2
N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors	2
Multivariate phase‐dependent optimization of bioprocesses boosts performance and quality—Why timing (of exposure) matters	2
Analytical characterization of broadly neutralizing antibody CAP256LS heavy chain clipping during manufacturing development	2
Understanding the effect of temperature downshift on CHO cell growth, antibody titer and product quality by intracellular metabolite profiling and in	2
Innovative methodology for flexible monitoring of various bioprocesses by using pre‐existing Raman data coupled with automated transfer learning technique	2
Gene copy number, gene configuration and LC / HC mRNA ratio impact on antibody productivity and pr	2
RETRACTION: A Novel pH‐Responsive Nanoniosomal Emulsion for Sustained Release of Curcumin from a Chitosan‐Based Nanocarrier: Emphasis on the Concurrent Improvement of Loading, Sustained Release, and A	2