Biotechnology Progress

Article	Citations
Dynamic flux balance analysis of 1,3‐propanediol production by clostridium butyricum fermentation	82
From protein structure to an optimized chromatographic capture step using multiscale modeling	56
Design of experiments‐guided membrane capture facilitates integration of continuous virus inactivation using Phi6 as a surrogate virus in an intensified downstream process	54
Proteomics‐based method to comprehensively model the removal of host cell protein impurities	33
The effects of the chemical modification on immobilized lipase features are affected by the enzyme crowding in the support	31
Optimized pulse‐feeding fed‐batch fermentation for enhanced lignin to polyhydroxyalkanoate transformation	31
Issue Information	30
Acetate accumulation and regulation by process parameters control in Chinese hamster ovary cell culture	30
Pre‐folding purification procedures for inclusion body‐derived non‐tagged cationic recombinant proteins with multiple disulfide bonds for efficient refolding	29
A practical framework for clone selection, media‐feed screening, and upstream process parameter optimization for a mAb CHO cell culture process	29
Dynamic pH profiles drive higher cell‐specific and volumetric productivity	26
NS0 and CHO cell lines generate distinct quality profiles for non‐originator NISTmAbs	26
Exploring the design space for T riton X ‐100 substitutes in viral inactivation applications	24
Machine learning‐assisted clone selection for intensified cell culture processes	24
Depth filtration for clarification of intensified lentiviral vector suspension cell culture	23
Enrichment of full AAV capsids by preparative strong anion exchange chromatography	21
High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions	21
Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations	19
Issue Information	19
The production of succinate with more CO 2 fixation reactions facilitate	19
Evaluating multiproduct chromatography Protein A resin reuse for monoclonal antibodies in biopharmaceutical manufacturing	18
Current status of platelet manufacturing in 3D or bioreactors	17
Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations	17
Genetically engineered neural stem cells (NSCs) therapy for neurological diseases; state‐of‐the‐art	17
Removal of chromatin by salt‐tolerant endonucleases for production of recombinant measles virus	17

Hands‐free from inoculation to harvest: Microbial fermentation with multivariate model to automate induction of recombinant protein expression	16
Modification of microstructure and selected physicochemical properties of bacterial cellulose produced by bacterial isolate using hydrocolloid‐fortified Hestrin–Schramm	16
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Raman spectroscopy and one‐dimensional convolutional neural network modeling as a real‐time monitoring tool for in vitro transaminase‐catalyzed synthesis of a pharmaceutically relevant amine precursor	16
Development of virus‐like particles‐based vaccines against coronaviruses	15
Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐ CLOVER establishes enhanced CHO	15
Correlation of microbial community structure and volatile flavor compounds during corn yellow wine fermentation	15
Immobilized enzymes as potent antibiofilm agent	15
Correction to “Investigation of the hepatic respiration and liver zonation on rat hepatocytes using an integrated oxygen biosensor in a microscale device”	15
Wound dressing based on PVA nanofiber containing silk fibroin modified with GO /	14
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Enhancing mRNA vaccine production: Optimization of in vitro transcription for improved yield and purity	14
Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach	13
Characterization of lipoprotein supplement and influence of its oxidized lipid content on cell culture performance and monoclonal antibody production by a SP2	13
Impact of X ‐ray irradiation as an equivalent alternative to gamma for sterilization of single‐use bioprocessing polymers	12
Bioburden detection on surface and water samples in a rapid, ultra‐sensitive and high‐throughput manner	12
Non‐sterile cultivation of Yarrowia lipolytica in fed‐batch mode for the production of lipids and biomass	12
The development of an automated microscope image tracking and analysis system	12
Automation of an integrated micro‐scale platform for monoclonal antibody process development by incorporation of a depth filter mimic	11
Enhanced production of isobutyl and isoamyl acetate using Yarrowia lipolytica	11
Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture	10
Shear stress and microRNAs for better metastatic cancer management	10
Primary clarification of mammalian cell culture fluid using enhanced sedimentation on inclined surfaces inside the single‐use disposable Sudhin BioSettler150	10
Petroleum coke supplementation for enhanced biogas production and phosphate removal under mesophilic conditions	10
A simple electrochemical genosensor based on polythiophene acetic acid film for detection of Schistosoma mansoni	10
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803	10
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cel	10
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell	9
Bioengineered chimeric tRNA / pre‐miRNAs as prodrugs in cancer therapy	9
Accelerating IND‐enabling toxicology studies using protein products from stable pools or pools of clones in Chinese hamster ovary cells	9
Non‐thermal plasma decontamination of microbes: a state of the art	9
Surface decoration of low molecular weight polyethylenimine ( LMW PEI ) by phthalated dextrin for improved delivery of interleukin‐12 plasmid	9
When will we have a clone? An industry perspective on the typical CLD timeline	9
Improving trans‐cinnamic acid production in a model cyanobacterium	9
Use of single analytic tool to quantify both absolute N‐glycosylation and glycan distribution in monoclonal antibodies	8
Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions	8
Comparing in silico flowsheet optimization strategies in biopharmaceutical downstream processes	8
General strategies for IgG‐like bispecific antibody purification	8
Modeling scalability of impurity precipitation in downstream biomanufacturing	8
Issue Information	8
Transmission of poly( dT 60 )	7
Optimization of amino acid composition in CHO cell perfusion medium using definitive screening design and	7
High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures	7
A novel synthetic sRNA promoting protein overexpression in cell‐free systems	7
Issue Information	7
A size‐exclusion chromatography fingerprinting workflow for the development of flow‐through polishing operations for mAbs derived from continuous precipitation processes	7
Biotechnological potential of yeast cell wall: An overview	7
Amino acid abundance and composition in cell culture medium affects trace metal tolerance and cholesterol synthesis	7
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic producti	7
Comparison of multi‐column chromatography configurations through model‐based optimization	7

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Process optimization mitigated the retention loss of an Fc‐fusion protein during ultrafiltration/diafiltration	6
A case study showing the role of hydrophobicity variants and other enriched mAb proteoforms on filterability through a virus filter with productivity	6
Optimization of carotenoid production by Umbelopsis ramanniana	6
RETRACTION : Comparison of Expression Optimization of New Derivative of staphylokinase ( SAK ‐	6
Impact of bioreactor process parameters and yeast biomass on Raman spectra	6
Disulfide bond reduction of antibodies during clarification: A bench‐scale assay and a mitigation tool	6
Exploring the potential of machine learning for more efficient development and production of biopharmaceuticals	6
Artificial intelligence and machine learning‐assisted digital applications for biopharmaceutical manufacturing	6
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of	6
Issue Information	6
Transcriptomic functional characterization of recombinant adeno‐associated virus producing cell line adapted to suspension‐growth	6
A strategy for successful dual‐species protein expression of genes with non‐optimal codon usage destined for bacterial and yeast cell factories	6
Continuous glucose feedback control using Raman spectroscopy and deep learning models for biopharmaceutical processes	6
New technical concept for alternating tangential flow filtration in biotechnological cell separation processes	6
Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process	6
Predictive modeling of single pass tangential flow filtration for continuous biomanufacturing	6
An adaptive modeling approach using spiking‐augmentation method to improve chemometric model performance in bioprocess monitoring	6
Size‐based analysis of virus removal filter fouling using fractionated protein aggregates	6
Harnessing cell aggregates for enhanced adeno‐associated virus manufacturing: Cultivation strategies and scale‐up considerations	5
A systematic approach to reduce intraocular pressure for the treatment of glaucoma	5
Integrated approach for purification of uricase and protease from Bacillus licheniformis by TPP and	5
Application of a fluorescent dye‐based microfluidic sensor for real‐time detection of mAb aggregates	5
Polyhydroxyalkanoates and exopolysaccharides: An alternative for valuation of the co‐production of microbial biopolymers	5
Utilizing C ole– C ole parameters for in‐line feedback: Cell culture process adjustments based on	5
Pharmaceutical proteins at the interfaces and the role of albumin	5
Purification of antisense oligonucleotides using hydrophobic interaction chromatography	5
A synthetic platform for developing recombinant adeno‐associated virus type 8 producer cell lines	5
Effects of LEDs, macronutrients and culture conditions on biomass and artemisinin production using Artemisia annuaL. suspension cultures	5
Design of a bactericidal hydrogel scaffold containing genipin crosslinked HF ‐18 peptide	5
Enhanced cell‐specific productivity through delayed supplementation of antioxidants in intensified processes	5
Special issue on integrated continuous biomanufacturing	5
Issue Information	5
Intensified perfusion culture (IPC) reduced recombinant protein fragmentation	5
Modulation of nutrient precursors for controlling metabolic inhibitors by genome‐scale flux balance analysis	5
Issue Information	5
A new method to immobilize urease in silk fibroin membrane by unidirectional nanopore dehydration	5
Adapting virus filtration to continuous processing: Effects of product and process variability on filtration performance	5
Chitosan based hybrid superabsorbent for controlled drug delivery application	5
Efficient optimization of time‐varying inputs in a fed‐batch cell culture process using design of dynamic experiments	5
Effect of cell retention techniques in Komagataella phaffii lab‐scale continuous processes	5
Isoelectric point ( pI )‐based phase separation ( pI‐BPS ) purification of elastin‐like polypeptid	4
Impedimetric nanoimmunosensor platform for aflatoxin B1 detection in peanuts	4
Simplifying stable CHO cell line generation with high probability of monoclonality by using microfluidic dispensing as an alternative to fluorescence	4
Triton CG ‐110 as an alternative for cell lysis in manufacturing of adeno‐associated virus ‐based	4
Predictive mechanistic model for separation of monoclonal antibody, fab fragment, and aggregate species on multimodal chromatography	4
Evaluating the impact of ionic strength on retention of minute virus of mice during small virus‐retentive filtration	4
Optimizing monoclonal antibody biosimilar production via transfer and active learning for targeted quality profiles	4
Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cell lines	4
Rapid development of a transferable R aman model using high‐throughput cell culture for monitoring monoclonal antibody titer	4
Application of Ambr15 system for simulation of entire SARS‐CoV ‐2 vaccine production process involving macrocarriers	4
Difficult‐to‐express antigen generation through a co‐expression and disassociation methodology	4
Single‐cell RNA sequencing reveals population‐based shifts in gene expression heterogeneity in an unstable, clonally derived	4
Performance of large virus removal filters during AAV processing: Influence of flux and process disruptions	4
A low‐cost, high‐throughput microfluidic nano‐culture platform for functional metagenomics	4
Unveiling microbial and microalgal chassis for therapeutic and diagnostic protein expression	4
Model validation and selection in metabolic flux analysis and flux balance analysis	4
Real‐time online monitoring of insect cell proliferation and baculovirus infection using digital differential holographic microscopy and machine learning	4
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Issue Information	4
Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support	4
Managing integrated continuous bioprocesses in real time: Deviations in product quality	4
Development of multiparametric bioprinting method for generation of 3D printed cell‐laden structures	4
Synthetic modeling: A cell‐free approach for faster implementation of Raman spectroscopy in cell culture	4
Application of fucosylation inhibitors for production of afucosylated antibody	4
The combination of oleic acid, linoleic acid, palmitoleic acid, and α‐linolenic acid promoted the expansion of NK ‐92 cells in vitro	4
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of	4
Effect of flux and shear rate on E. coli recovery in tangential flow filtration through a single hollow fiber	4
Cryopreservation practices in clinical and preclinical iPSC ‐based cell therapies: Current challenges and future directions	4
Data‐driven design of deterministic lateral displacement microfluidic devices for CTC separation	4
Aptamer‐functionalized quantum dots as theranostic nanotools against cancer and bacterial infections: A comprehensive overview of recent trends	4
Just passing through: Deploying aquaporins in microbial cell factories	4
Relationship between intracellular protein denaturation and irreversible inactivation of Saccharomyces pastorianus by low‐pressure carbon dioxide microbu	4
Environmental conditions during glycerol bioconversion affect 3‐hydroxypropionic acid bioproduction by Limosilactobacillus reuteri DSM	4
Analytical characterization of host‐cell‐protein‐rich aggregates in monoclonal antibody solutions	3
High‐throughput in silico workflow for optimization and characterization of multimodal chromatographic processes	3
Enhancement of D‐mannitol production by fine‐tuned expression of mannitol‐1‐phosphate dehydrogenase in Synechocystis sp. PCC6803	3
Identification of infectious viruses for risk‐based virus testing of CHO unprocessed bulk using next‐generation sequencing	3
Tangential flow filtration‐facilitated purification of human red blood cell membrane fragments and its preferential use in removing unencapsulated material from resealed red blood cell ghosts compared	3
Comprehensive studies on building a scalable downstream process for mRNAs to enable mRNA therapeut	3

Correction to “Soluble production and function of vascular endothelial growth factor/basic fibroblast growth factor complex peptide”	3
An updated overview of the application of CAR‐T cell therapy in neurological diseases	3
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Design and optimization of membrane chromatography for monoclonal antibody charge variant separation	3
Addressing challenging impurities in fusion proteins: A comprehensive review and cost‐effective strategy for HCP and low molecular weight species con	3
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Real time on‐line amino acid analysis to explore amino acid trends and link to glycosylation outcomes of a model monoclonal antibody	3
Accelerated CMC workflows to enable speed to clinic in the COVID ‐19 era: A multi‐company view fro	3
Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives	3
Transcriptomic features reveal molecular signatures associated with recombinant adeno‐associated virus production in HEK293 cells	3
Recent trends in biotechnological production, engineering, and applications of lysophospholipases	3
An automated high inoculation density fed‐batch bioreactor, enabled through N‐1 perfusion, accommodates clonal diversity and doubles titers	3
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Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies	3
Development of chondrocyte‐laden alginate hydrogels with modulated microstructure and properties for cartilage regeneration	3
Issue Information	3
Assessment of membrane‐based downstream purification processes as a replacement to traditional resin bead for monoclonal antibody purification	3
Modeling the effect of oxidative stress on Bordetella pertussis fermentations	3
Glass microspargers as effective frit spargers in single use bioreactors	3
Multi‐omics kinetic analysis of recombinant adeno‐associated virus production by plasmid transfection of HEK293 cells	3
Issue Information	3
Biotransformation of maize bran‐derived ferulic acid to vanillin using an adapted strain of Amycolatopsis sp. ATCC 39116	3
Mechanistic modeling, simulation, and optimization of mixed‐mode chromatography for an antibody polishing step	3
Process intensification of the baculovirus expression vector system using a perfusion process with a low multiplicity of infection at high cell conce	3
Issue Information	3
Exploring the biocatalysis of psilocybin and other tryptamines: Enzymatic pathways, synthetic strategies, and industrial implications	3
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor suppl	3
Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals	3
Integrated micro‐scale protein a chromatography and Low pH viral inactivation unit operations on an automated platform	3
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing	3
Advances in polymer‐based cell encapsulation and its applications in tissue repair	3
Evaluation of lentiviral vector stability and development of ion exchange purification processes	3
Host cell proteins in monoclonal antibody processing: Control, detection, and removal	3
Highly iterated palindrome 1 sequence improves Synechococcus sp. PCC 7002 transformation efficiencies	3
Changes to culture pH and dissolved oxygen can enhance chimeric antigen receptor T‐cell generation and differentiation	3
Sources and methods of manufacturing xanthan by fermentation of various carbon sources	3
Integration of Planova filters in manufacturing processes of biologicals improve the virus safety effectively: A review of publicly available data	3
Xanthan gum enhances peripheral blood CIK cells cytotoxicity in serum‐free medium	2
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling	2
Advanced strategies in glycosylation prediction and control during biopharmaceutical development: Avenues toward industry 4.0	2
Issue Information	2
Modulation of the cell cycle and inhibition of histone deacetylases by small molecules increase recombinant adeno‐associated virus productivity across different HEK293 cell lines	2
Mesenchymal stem cells derived‐exosomes enhanced amniotic membrane extract promotes corneal keratocyte proliferation	2
Investigation of a failed DNA assay leads to identification of an unexpected contaminant in a commonly used chromatography buffer	2
Issue Information	2
A deep‐well plate enabled automated high‐throughput cell line development platform	2
Dentin regeneration based on tooth tissue engineering: A review	2
Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments	2
In silico optimization of a challenging bispecific antibody chromatography step	2
Use of live digital twin (shadow) soft sensor to monitor membrane degradation in continuous manufacturing single pass tangential flow filtration	2
Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization	2
Understanding adsorption behavior of Polysorbate‐20 to sterile filters in therapeutic proteins final filtration process	2
Continuous CTC separation through a DEP‐based contraction–expansion inertial microfluidic channel	2
Understanding the effect of temperature downshift on CHO cell growth, antibody titer and product quality by intracellular metabolite profiling and in	2
Advancing multiproduct resin reuse for development and clinical manufacturing of an antibody‐based therapeutic	2
Secondary feed filtration and storage conditions influence trace element availability and process performance at 2000  L scale	2
Image analysis method for measurement and prediction of intra‐matrix IgG diffusion	2
Implementation of mDoE ‐methods to a microcarrier‐based expansion processes for mesenchymal stem cells	2
A digital shadow of CAR T cell expansion in a perfusion bioreactor: Informing optimal harvest times for autologous cell therapy	2
Development of targeted gene delivery system based on liposome and PAMAM dendrimer functionalized with hyaluronic acid and T	2
Characterization of apoptosis in fed‐batch cultures highlights the impact of timely process intervention on improving recombinant protein productivity	2
5‐Aza combined with VPA reprograms human T lineage acute leukemia Jurkat cells into B‐cell‐like cells by epigenetic activation of	2
N‐1 semi‐continuous transient perfusion in shake flask for ultra‐high density seeding of CHO cell cultures in benchtop bioreactors	2
A design space for the filtration of challenging monoclonal antibodies using Planova™ S20N , a new regenerated cellulose virus removal filter	2
A modular and multi‐functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing	2
Analytical characterization of broadly neutralizing antibody CAP256LS heavy chain clipping during manufacturing development	2
Scalable desolvation synthesis, storage stability, and comprehensive biophysical characterization of human hemoglobin nanoparticles as a potential hemoglobin‐based oxygen carrier	2
Multivariate phase‐dependent optimization of bioprocesses boosts performance and quality—Why timing (of exposure) matters	2
Efficient fermentative production of lactodifucotetraose by controlling sequential glycosyltransferase reactions in Escherichia coli	2
A comparison of different intensified upstream processes highlighting the advantage of WuXi Biologics' Ultra‐high Productivity platform ( WuXiUP	2
Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase	2
Use of ultrafiltration/diafiltration for the processing of antisense oligonucleotides	2
Enhancing cryopreservation of human induced pluripotent stem cells: Bottom‐up versus conventional freezing geometry	2
“Just add small molecules” cell‐free protein synthesis: Combining DNA template and cell extract preparation into a single fermentation	2
A PVA /chitosan/gelatin scaffold incorporating salicylic acid‐loaded TiO ₂ nanoparticles enhances	2
Tangential flow filtration facilitated fractionation of polymerized human serum albumin: Insights into the effects of molecular size on biophysical properties	2
Correction to “Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in	2
Effect of pH, NaCl concentration, and mAb concentration of feed solution on the filterability of Planova™ 20N and Planova™ BioEX	2