OOIR: Observatory of International Research

Papers

(The median citation count of CRISPR Journal is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)

Article	Citations
CRISPR-Cas Gene Perturbation and Editing in Human Induced Pluripotent Stem Cells	408
Establishing CRISPR-Cas Technology in Pakistan	58
The CRISPR Chronicles and the Power of Storytelling	45
Exploring the Cytoplasmic Retention of CRISPR-Cas9 in Eukaryotic Cells: The Role of Nuclear Localization Signals and Ribosomal Interactions	41
A Field Day for Gene-Edited Brassicas and Crop Improvement	38
Rapid and Technically Simple Detection of SARS-CoV-2 Variants Using CRISPR Cas12 and Cas13	36
Search for Origins of Anti-CRISPR Proteins by Structure Comparison	35
Correction to: Human Germline and Heritable Genome Editing: The Global Policy Landscape by Baylis et al. The CRISPR Journal 2020;3(5): 365–377; DOI: 10.1089/crispr.2020.0082	33
Deep Sequencing Reveals the Comprehensive CRISPR-Cas9 Editing Spectrum in Bombyx mori	31
Increasing Genome Editing Efficiency of Cas9 Nucleases by the Simultaneous Use of Transcriptional Activators and Histone Acetyltransferase Activator	31
CRISPR and the Plant Pathologists' Holy Grail	29
Rosalind Franklin Society Proudly Announces the 2022 Award Recipient for The CRISPR Journal	29
Transversion Expansion of Base Editing	28
Special Issue: CRISPR's Path to the Clinic	26
What if Bacteria Could Tell Us What They Have Seen?	25
A CRISPR Landing for Genome Rewriting at Locus-Scale	25
Spotlight on Genetic Design in a Spotted Wing Crop Killer	24
Model System to Analyze RNA-Mediated DNA Repair in Mammalian Cells	24
Functional and Phylogenetic Diversity of Cas10 Proteins	23
Modified Cas9-Guided Oxford Nanopore Technology Sequencing Uncovers Single and Multiple Transgene Insertion Sites in a Zebrafish Melanoma Model	22
CRISPR Milestones for Sustainable Agriculture and Forestry	22
Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing	21
A Protein-Cutting CRISPR Complex Caught in Action	21
It's a Knockout for Cat Allergies?	21
Structural Investigation of Self-Assembly and Target Binding of Anti-CRISPR AcrIIC2	19

CRISPR Adventures in China	19
Pseudogene-Mediated Gene Conversion After CRISPR-Cas9 Editing Demonstrated by Partial CD33 Conversion with SIGLEC22P	18
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels	18
Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types	16
Prime Editing in Mammals: The Next Generation of Precision Genome Editing	16
Efficient Generation of SOCS2 Knock-Out Sheep by Electroporation of CRISPR-Cas9 Ribonucleoprotein Complex with Dual-sgRNAs	14
CRISPR Comparison Toolkit: Rapid Identification, Visualization, and Analysis of CRISPR Array Diversity	14
Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing	13
Rationally Designed Base Editors for Precise Editing of the Sickle Cell Disease Mutation	13
Optimization of Genomewide CRISPR Screens Using AsCas12a and Multi-Guide Arrays	13
A Mutation-Agnostic Hematopoietic Stem Cell Gene Therapy for Metachromatic Leukodystrophy	12
The CRISPR Revolution in the Drug Discovery Workflow: An Industry Perspective	12
Locus-Specific Genomic DNA Purification Using the CRISPR System: Methods and Applications	12
GOANA: A Universal High-Throughput Web Service for Assessing and Comparing the Outcome and Efficiency of Genome Editing Experiments	12
CRISPRa-Mediated Upregulation of scn1laa During Early Development Causes Epileptiform Activity and dCas9-Associated Toxicity	11
Induction and Elimination of Prophages Using CRISPR Interference	11
Allele-Specific Inactivation of an Autosomal Dominant Epidermolysis Bullosa Simplex Mutation Using CRISPR-Cas9	11
High-Throughput Imaging of CRISPR- and Recombinant Adeno-Associated Virus–Induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells	11
Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for The CRISPR Journal	10
Bacterial Retrons Enable Precise Gene Editing in Human Cells	10
First Drug Approval Rises the CRISPR Tide	10
Warrior Spirit: An Interview with Victoria Gray, Sickle Cell Pioneer	10
Hairpin Internal Nuclear Localization Signals in CRISPR-Cas9 Enhance Editing in Primary Human Lymphocytes	9
Gene Editing Corrects In Vitro a G > A GLB1 Transition from a GM1 Gangliosidosis Patient	9
CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2	9
ON-Target Adverse Events of CRISPR-Cas9 Nuclease: More Chaotic than Expected	9
Viral Delivery of Compact CRISPR-Cas12f for Gene Editing Applications	9
Initial Characterization of 12 New Subtypes and Variants of Type V CRISPR Systems	8
Auditing the Editor: A Review of Key Translational Issues in Epigenetic Editing	8
Dead Cas9–sgRNA Complex Shelters Vulnerable DNA Restriction Enzyme Sites from Cleavage for Cloning Applications	8
Inference of CRISPR Edits from Sanger Trace Data	8
The CRISPR Patent Landscape: Focus on Chinese Researchers	8
Navigating Viral Space with CRISPR Technologies	8
Expression of Cas9 in a Syngeneic Model of Primary Central Nervous System Lymphoma Induces Intracerebral NK and CD8 T Cell-Mediated Lymphoma Cell Lysis Via Perforin	8
A CRISPR-dCas9 System for Assaying and Selecting for RNase III Activity In Vivo in Escherichia coli	8
Gene Editing and Rett Syndrome: Does It Make the Cut?	8
Challenging the Boundaries Between Treatment, Prevention, and Enhancement in Human Genome Editing	7
Increasing Gene Editing Efficiency for CRISPR-Cas9 by Small RNAs in Pluripotent Stem Cells	7
Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells by Zhang et al. The CRISP	7
Curing “GFP-itis” in Bacteria with Base Editors: Development of a Genome Editing Science Program Implemented with High School Biology Students	7
Clinical Milestone for CRISPR Medicines	7
Novel and Engineered Type II CRISPR Systems from Uncultivated Microbes with Broad Genome Editing Capability	7
Correction to: Clonally Selected Lines After CRISPR-Cas Editing Are Not Isogenic by Panda et al. The CRISPR Journal, 2023;6(2):176–182; DOI: 10.1089/crispr.2022.0050	7
Green Fluorescent Protein Tagged Polycistronic Reporter System Reveals Functional Editing Characteristics of CRISPR-Cas	6
Genetic Manipulation of a CAST of Characters in a Microbial Community	6
Gene-Edited Fluorescent and Mixed Cerebral Organoids	6
Enhancing Precision and Efficiency of Cas9-Mediated Knockin Through Combinatorial Fusions of DNA Repair Proteins	6
A Taxonomic and Phylogenetic Classification of Diverse Base Editors	6
Vulnerability and the Ethics of Human Germline Genome Editing	6
Correction to: Programmable RNA Targeting Using CasRx in Flies, by Buchman, et al. The CRISPR Journal 2020;3(3)164–176; doi: 10.1089/crispr.2020.0018	6

Base Editors Flex Sights on Sickle-Cell Disease	6
Universal Gene Correction Approaches for β-hemoglobinopathies Using CRISPR-Cas9 and Adeno-Associated Virus Serotype 6 Donor Templates	6
Acknowledgment of Reviewers 2022	6
Acknowledgment of Reviewers 2023	6
Five Years of Progress in CRISPR Clinical Trials (2019–2024)	5
CALITAS: A CRISPR-Cas-aware ALigner for In silico off-TArget Search	5
Acknowledgment of Reviewers 2024	5
Regulatory Considerations for Clinical Trial Applications with CRISPR-Based Medicinal Products	5
Comparison of Gene-Editing Approaches for Severe Congenital Neutropenia-Causing Mutations in the ELANE Gene	5
Correction to: A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors by Chen et al. The CRISPR Journal, 2023;6(6):570–582; DO	5
Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration	5
Discovery of Diverse CRISPR Leader Motifs, Putative Functions, and Applications for Enhanced CRISPR Detection and Subtype Annotation	5
CRISPR-Cas9 Gene Editing Protects from the A53T-SNCA Overexpression-Induced Pathology of Parkinson's Disease In Vivo	5
The DNA-Dialogue: A Broad Societal Dialogue About Human Germline Genome Editing in the Netherlands	5
Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes	5
Increasing Knockin Efficiency in Mouse Zygotes by Transient Hypothermia	5
CLASH of the Titans: How CAR-T Cells Can Triumph Over Tumors	5
Impact of KIT Editing on Coat Pigmentation and Fresh Meat Color in Yorkshire Pigs	5
Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection	5
Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering	5
CRISPR Hits Home in a First-in-Human Study	5
Miniature CRISPR-Cas12f1-Mediated Single-Nucleotide Microbial Genome Editing Using 3′-Truncated sgRNA	4
Genome Editing Therapy for the Blood: Ex Vivo Success and In Vivo Prospects	4
Microfluidic Enrichment and Computational Analysis of Rare Sequences from Mixed Genomic Samples for Metagenomic Mining	4
New Editing Tools for Gene Therapy in Inherited Retinal Dystrophies	4
Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells	4
Early Detection of Wildlife Disease Pathogens Using CRISPR-Cas System Methods	4
Efficient C-to-G Base Editing with Improved Target Compatibility Using Engineered Deaminase–nCas9 Fusions	4
Rapid Variant Pathogenicity Analysis by CRISPR Activation of CRB1 Gene Expression in Patient-Derived Fibroblasts	4
Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth	4
CRISPR-AsCas12a Efficiently Corrects a GPR143 Intronic Mutation in Induced Pluripotent Stem Cells from an Ocular Albinism Patient	4
Determination of Factors Driving the Genome Editing Field in the CRISPR Era Using Bibliometrics	4
CASPER: An Integrated Software Platform for Rapid Development of CRISPR Tools	4
Repurposing an Endogenous CRISPR-Cas System to Generate and Study Subtle Mutations in Bacteriophages	4
Gene Editing of Checkpoint Molecules in Cord Blood-Derived Dendritic Cells and CD8⁺ T Cells Using CRISPR-Cas9	4
Measuring the Impact of Genetic Heterogeneity and Chromosomal Inversions on the Efficacy of CRISPR-Cas9 Gene Drives in Different Strains of Anopheles gambiae	4
Sensitive and Easy-Read CRISPR Strip for COVID-19 Rapid Point-of-Care Testing	4
Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas	4
Give Cas a Chance: An Actionable Path to a Platform for CRISPR Cures	4
Breaking the Rules	4
Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells	4
Response to Cook et al. re: Novel Off-Targeting Events Identified After Genome Wide Analysis of CRISPR-Cas Edited Pig	4
Natural Nucleoside Modifications in Guide RNAs Can Modulate the Activity of the CRISPR-Cas9 System In Vitro	3
Germline Editing of Drosophila Using CRISPR-Cas9-Based Cytosine and Adenine Base Editors	3
CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa	3
A Novel System for Simple Rapid Adenoviral Vector Construction to Facilitate CRISPR/Cas9-Mediated Genome Editing	3
Establishment of a Cleavage-Based Single-Plasmid Dual-Luciferase Surrogate Reporter for the Cleavage Efficiency Evaluation of CRISPR-Cas12a Systems and Its Primary Application	3
Identification and Evolution of Cas9 tracrRNAs	3
CRISPR-Mediated Synergistic Epigenetic and Transcriptional Control	3
Catalytically Enhanced Cas9 Through Directed Protein Evolution	3
Insertion of the Icelandic Mutation (A673T) by Prime Editing: A Potential Preventive Treatment for Familial and Sporadic Alzheimer's Disease	3
Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila	3
Predicting Mutations Generated by Cas9, Base Editing, and Prime Editing in Mammalian Cells	3
Increasing the Targeting Scope of CRISPR Base Editing System Beyond NGG	3
Optimizing Integration and Expression of Transgenic Bruton's Tyrosine Kinase for CRISPR-Cas9-Mediated Gene Editing of X-Linked Agammaglobulinemia	3
A Novel Set of Cas9 Fusion Proteins to Stimulate Homologous Recombination: Cas9-HRs	3
gEL DNA: A Cloning- and Polymerase Chain Reaction–Free Method for CRISPR-Based Multiplexed Genome Editing	3
Strategies and Protocols for Optimized Genome Editing in Potato	3
Special Issue: CRISPR Trials	2
The CRISPR Toolbox: The End of the Beginning	2
Reduction of Pre-Existing Adaptive Immune Responses Against SaCas9 in Humans Using Epitope Mapping and Identification	2
CRISPR-Cas9-Mediated Gene Editing of MYB28 Genes Impair Glucoraphanin Accumulation of Brassica oleracea in the Field	2
Enhanced Taq Variant Enables Efficient Genome Editing Testing and Mutation Detection	2
Diverse Class 2 CRISPR Effectors as Active Nucleases with Expanded Targeting Capabilities	2
CRISPR Gets Its Origin Story	2
Functional Study of the Type II-A CRISPR-Cas System of Streptococcus agalactiae Hypervirulent Strains	2
Enhanced Myogenesis by Silencing Myostatin with Nonviral Delivery of a dCas9 Ribonucleoprotein Complex	2
Genome Editing Headwinds: Can CRISPR Stay on Target?	2
CRISPR-Cas9 Technology as a Tool to Target Gene Drivers in Cancer: Proof of Concept and New Opportunities to Treat Chronic Myeloid Leukemia	2
Bio-Orthogonal Chemistry Conjugation Strategy Facilitates Investigation of N-methyladenosine and Thiouridine Guide RNA Modifications on CRISPR Activity	2
As CRISPR Technologies Ripen, What Should We Prioritize?	2
Implementing CRISPR-Cas9 Yeast Practicals into Biology Curricula	2
Unveiling Off-Target Mutations in CRISPR Guide RNAs: Implications for Gene Region Specificity	2
WHO: Are You Ready for Human Genome Editing?	2
Discovery and Characterization of Novel Type V Cas12f Nucleases with Diverse Protospacer Adjacent Motif Preferences	2
Multiplex gRNAs Synergically Enhance Detection of SARS-CoV-2 by CRISPR-Cas12a	2
Cas4–Cas1 Is a Protospacer Adjacent Motif–Processing Factor Mediating Half-Site Spacer Integration During CRISPR Adaptation	2
A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs	2
CRISPR Crops and Sustainable Agriculture	2