OOIR: Observatory of International Research

Papers

(The median citation count of CRISPR Journal is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-08-01 to 2025-08-01.)

Article	Citations
CRISPR-Cas Gene Perturbation and Editing in Human Induced Pluripotent Stem Cells	470
Exploring the Cytoplasmic Retention of CRISPR-Cas9 in Eukaryotic Cells: The Role of Nuclear Localization Signals and Ribosomal Interactions	49
Hairpin Internal Nuclear Localization Signals in CRISPR-Cas9 Enhance Editing in Primary Human Lymphocytes	48
Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types	43
Increasing Genome Editing Efficiency of Cas9 Nucleases by the Simultaneous Use of Transcriptional Activators and Histone Acetyltransferase Activator	37
Gene Editing Corrects In Vitro a G > A GLB1 Transition from a GM1 Gangliosidosis Patient	37
Inference of CRISPR Edits from Sanger Trace Data	36
CRISPR Comparison Toolkit: Rapid Identification, Visualization, and Analysis of CRISPR Array Diversity	36
Will Advances in Genetic Medicine Lessen the Need for Germline Modifications?	36
Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes	31
Acknowledgment of Reviewers 2022	29
A Taxonomic and Phylogenetic Classification of Diverse Base Editors	29
Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering	29
Curing “GFP-itis” in Bacteria with Base Editors: Development of a Genome Editing Science Program Implemented with High School Biology Students	28
Gene Editing and Rett Syndrome: Does It Make the Cut?	26
Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas	26
Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection	25
Opposing Human Genetic Engineering	25
Increasing the Targeting Scope of CRISPR Base Editing System Beyond NGG	23
Implementing CRISPR-Cas9 Yeast Practicals into Biology Curricula	20
Early Detection of Wildlife Disease Pathogens Using CRISPR-Cas System Methods	20
CRISPR Crops and Sustainable Agriculture	19
Comprehensive Analysis of CRISPR-Cas9 Editing Outcomes in Yeast Xanthophyllomyces dendrorhous	19
The Roles and Responsibilities of Scientific Journals in Research Governance: Editorial Policymaking at Nature and Springer Nature (1995–2023)	17
Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing	17

Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors	17
CRISPR à la carte	16
Evolutionary Biology and Gene Editing of Cat Allergen, Fel d 1	15
A Story of Perseverance: An Interview with Matthew Porteus	15
Widespread Impact of Natural Genetic Variations in CRISPR-Cas9 Outcomes	15
CRISPR Rewrites the Future of Medicine	15
Combined Theoretical, Bioinformatic, and Biochemical Analyses of RNA Editing by Adenine Base Editors	14
Evolution of Type IV CRISPR-Cas Systems: Insights from CRISPR Loci in Integrative Conjugative Elements of Acidithiobacillia	14
Automated Good Manufacturing Practice-Compatible CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells for Clinical Treatment of β-Hemoglobinopathies	14
Specific High-Sensitivity Enzymatic Reporter UnLOCKing-Mediated Detection of Oncogenic BCR::ABL1 and EGFR Rearrangements	13
Engineered Antiviral Sensor Targets Infected Mosquitoes	13
dCas9 Tells Tales: Probing Gene Function and Transcription Regulation in Cancer	13
Gene Editing in the Chagas Disease Vector Rhodnius prolixus by Cas9-Mediated ReMOT Control	13
Engineering CjCas9 for Efficient Base Editing and Prime Editing	12
Institutional Bioethics: From Critical Interdisciplinarity to Cosmopolitanism	12
CRISPR Momentum in the Clinic and the Field	12
Rational Engineering of CRISPR-Cas9 Nuclease to Attenuate Position-Dependent Off-Target Effects	12
Enhancements of the CRISPR-Cas System in the Silkworm Bombyx mori	11
Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines	11
Fanzors: Mysterious TnpB-Like Bacterial Transposon-Related RNA-Guided DNA Nucleases of Eukaryotes	11
A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors	11
Spotlight on Genetic Design in a Spotted Wing Crop Killer	10
A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance	10
A PCR-Induced Mutagenesis-Restriction Fragment Length Polymorphism Method for the Detection of CRISPR-Induced Indels	10
Affordable Pricing of CRISPR Treatments is a Pressing Ethical Imperative	10
It's a Knockout for Cat Allergies?	9
Warrior Spirit: An Interview with Victoria Gray, Sickle Cell Pioneer	9
Introducing Faith-Based Claims to Ethical Discourse on Scientific Matters	9
Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing	9
The CRISPR Revolution in the Drug Discovery Workflow: An Industry Perspective	9
Acknowledgment of Reviewers 2023	9
A Protein-Cutting CRISPR Complex Caught in Action	9
Rapid and Technically Simple Detection of SARS-CoV-2 Variants Using CRISPR Cas12 and Cas13	9
Bacterial Retrons Enable Precise Gene Editing in Human Cells	9
CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2	9
Genome Editing Therapy for the Blood: Ex Vivo Success and In Vivo Prospects	8
Rapid Variant Pathogenicity Analysis by CRISPR Activation of CRB1 Gene Expression in Patient-Derived Fibroblasts	8
CRISPR-Cas9 Gene Editing Protects from the A53T-SNCA Overexpression-Induced Pathology of Parkinson's Disease In Vivo	8
Navigating Viral Space with CRISPR Technologies	8
Genome Editing Headwinds: Can CRISPR Stay on Target?	8
Five Years of Progress in CRISPR Clinical Trials (2019–2024)	8
The New Old Colonialism	7
Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila	7
Correction to: APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels by Rieffer et al. The CRISPR Journal, 2023;6(5):430–446; DOI: 10.1089/crispr.2023.0027	7
Diverse Class 2 CRISPR Effectors as Active Nucleases with Expanded Targeting Capabilities	7
Reduction of Pre-Existing Adaptive Immune Responses Against SaCas9 in Humans Using Epitope Mapping and Identification	7
Special Issue: Manipulating the Microbiome with CRISPR	7
Characterization of Research Support of Genome Editing Technologies and Transition to Clinical Trials	7
Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells	7
Moving Therapeutic Genome Editing into Global Clinical Trials and Medicine	7

Bio-Orthogonal Chemistry Conjugation Strategy Facilitates Investigation of N-methyladenosine and Thiouridine Guide RNA Modifications on CRISPR Activity	7
Correction to: Give Cas a Chance: An Actionable Path to a Platform for CRISPR Cures, by Fyodor D. Urnov [DOI: 10.1089/crispr.2024.0082]	6
Clonally Selected Lines After CRISPR-Cas Editing Are Not Isogenic	6
Celebrating a Decade of CRISPR-Fueled Genome Editing	6
Extreme Genome Editing	6
PASTE: The Way Forward for Large DNA Insertions	6
CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome	6
An Efficient and Cost-Effective Novel Strategy for Identifying CRISPR-Cas-Mediated Mutants in Plant Offspring	6
Special Issue: CRISPR Trials	6
Novel off-Targeting Events Identified after Genome Wide Analysis of CRISPR-Cas Edited Pigs	6
A Reset for Bioethics: A Statement from the Global Observatory for Genome Editing	6
Recalibrating Health Governance, Equity, and Reproductive Rights in India: A Case Study from the Global South	6
CRISPR-Cas9-Mediated Targeting of Multidrug Resistance Genes in Methicillin-Resistant Staphylococcus aureus	5
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels	5
CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors	5
Facing Systemic Uncertainty, Can the CRISPR Community Stay the Course?	5
High-Throughput Imaging of CRISPR- and Recombinant Adeno-Associated Virus–Induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells	5
Modified Cas9-Guided Oxford Nanopore Technology Sequencing Uncovers Single and Multiple Transgene Insertion Sites in a Zebrafish Melanoma Model	5
Optimization of Genomewide CRISPR Screens Using AsCas12a and Multi-Guide Arrays	5
Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing	5
Green Fluorescent Protein Tagged Polycistronic Reporter System Reveals Functional Editing Characteristics of CRISPR-Cas	5
Electroporation-Mediated Delivery of Cas9 Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes	5
Transversion Expansion of Base Editing	5
Viral Delivery of Compact CRISPR-Cas12f for Gene Editing Applications	5
Introducing Perspectives from the Global Observatory for Genome Editing	5
Genetic Manipulation of a CAST of Characters in a Microbial Community	5
AI and SynBio Meet CRISPR Heralding a New Genome Editing Era	4
Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells by Zhang et al. The CRISP	4
Discovery and Characterization of Novel Type V Cas12f Nucleases with Diverse Protospacer Adjacent Motif Preferences	4
Toward a Global Deliberative System for Genome Editing	4
Enhanced Myogenesis by Silencing Myostatin with Nonviral Delivery of a dCas9 Ribonucleoprotein Complex	4
Predicting Mutations Generated by Cas9, Base Editing, and Prime Editing in Mammalian Cells	4
CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa	4
CRISPR Hits Home in a First-in-Human Study	4
Response to Cook et al. re: Novel Off-Targeting Events Identified After Genome Wide Analysis of CRISPR-Cas Edited Pig	4
As CRISPR Technologies Ripen, What Should We Prioritize?	4
Modulating CRISPR-Cas Genome Editing Using Guide-Complementary DNA Oligonucleotides	4
Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration	4
Novel and Engineered Type II CRISPR Systems from Uncultivated Microbes with Broad Genome Editing Capability	4
Science with Society at Science Gallery Bengaluru	4
Genome Editing in Apicomplexan Parasites: Current Status, Challenges, and Future Possibilities	4
CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito, Aedes aegypti	3
Acknowledgment of Reviewers 2021	3
JACKIE: Fast Enumeration of Genome-Wide Single- and Multicopy CRISPR Target Sites and Their Off-Target Numbers	3
Advances in Gene Therapy for Sickle Cell Disease: From Preclinical Innovations to Clinical Implementation and Access Challenges	3
Genomic and Transcriptomic Analyses of Prime Editing Guide RNA–Independent Off-Target Effects by Prime Editors	3
Fast and Efficient Generation of Isogenic Induced Pluripotent Stem Cell Lines Using Adenine Base Editing	3
Highly Efficient One-Step Tagging of Endogenous Genes in Primary Cells Using CRISPR-Cas Ribonucleoproteins	3
Special Issue on CRISPR Diagnostics	3
Developing Bottom-Up Induced Pluripotent Stem Cell Derived Solid Tumor Models Using Precision Genome Editing Technologies	3
Biotechnologies in the World: On Global Asymmetries and the Need for Cosmopolitanism	3
Correction to: Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas by Burger et al. The CRISPR Journal,	3
Clinical Milestone for CRISPR Medicines	2
Search for Origins of Anti-CRISPR Proteins by Structure Comparison	2
Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for The CRISPR Journal	2
Establishment of a CRISPR-Cas9-Mediated Genome Editing System in Flax	2
Monitoring the Land and Sea: Enhancing Efficiency Through CRISPR-Cas Driven Depletion and Enrichment of Environmental DNA	2
Periodontal Disease Pathogens, Pathogenesis, and Therapeutics: The CRISPR-Cas Effect	2
Compact Cje3Cas9 for Efficient In Vivo Genome Editing and Adenine Base Editing	2
Functional Characterization of Type III-A CRISPR-Cas in a Clinical Human Methicillin-R Staphylococcus aureus Strain	2
Impact of KIT Editing on Coat Pigmentation and Fresh Meat Color in Yorkshire Pigs	2
Functional and Phylogenetic Diversity of Cas10 Proteins	2
Model System to Analyze RNA-Mediated DNA Repair in Mammalian Cells	2
Allele-Specific Inactivation of an Autosomal Dominant Epidermolysis Bullosa Simplex Mutation Using CRISPR-Cas9	2
Optimizing Genome Editing in Mollusks (Crassostrea gigas) in Vitro Validation of sgRNA and Identifying Key Factors Influencing Efficiency	2
Special Issue: Manipulating the Microbiome with CRISPR	2
The Promises and Pitfalls of CRISPR-Mediated Base Editing in Stem Cells	2
CRISPR Empowers Tree Bioengineering for a Sustainable Future	2
Efficient Generation of SOCS2 Knock-Out Sheep by Electroporation of CRISPR-Cas9 Ribonucleoprotein Complex with Dual-sgRNAs	2
Acknowledgment of Reviewers 2024	2
Gene-Edited Fluorescent and Mixed Cerebral Organoids	2
A Mutation-Agnostic Hematopoietic Stem Cell Gene Therapy for Metachromatic Leukodystrophy	2
Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells	2
CRISPR's Path to the Clinic	2
CRISPRclassify: Repeat-Based Classification of CRISPR Loci	2
Identification of a Guide RNA Targeting an Ultraconserved Element for Evaluation of Cas9 Genome Editors Across Mammalian Species	2
CRISPR and the Plant Pathologists' Holy Grail	2
Embryonic Stem Cell Research Oversight Committees: A Model for Oversight of Innovative Research?	2