OOIR: Observatory of International Research

Papers

(The median citation count of CRISPR Journal is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)

Article	Citations
Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types	419
Gene Editing Corrects In Vitro a G > A GLB1 Transition from a GM1 Gangliosidosis Patient	45
Increasing Genome Editing Efficiency of Cas9 Nucleases by the Simultaneous Use of Transcriptional Activators and Histone Acetyltransferase Activator	45
CRISPR-Cas Gene Perturbation and Editing in Human Induced Pluripotent Stem Cells	38
Hairpin Internal Nuclear Localization Signals in CRISPR-Cas9 Enhance Editing in Primary Human Lymphocytes	36
Exploring the Cytoplasmic Retention of CRISPR-Cas9 in Eukaryotic Cells: The Role of Nuclear Localization Signals and Ribosomal Interactions	36
CRISPR Comparison Toolkit: Rapid Identification, Visualization, and Analysis of CRISPR Array Diversity	35
Inference of CRISPR Edits from Sanger Trace Data	34
Editing Properties of Base Editors with SpCas9-NG in Discarded Human Tripronuclear Zygotes	33
A Taxonomic and Phylogenetic Classification of Diverse Base Editors	32
Curing “GFP-itis” in Bacteria with Base Editors: Development of a Genome Editing Science Program Implemented with High School Biology Students	30
Acknowledgment of Reviewers 2022	29
Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering	27
Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas	26
Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection	26
Gene Editing and Rett Syndrome: Does It Make the Cut?	26
Early Detection of Wildlife Disease Pathogens Using CRISPR-Cas System Methods	24
Increasing the Targeting Scope of CRISPR Base Editing System Beyond NGG	24
CRISPR Crops and Sustainable Agriculture	23
Implementing CRISPR-Cas9 Yeast Practicals into Biology Curricula	23
Comprehensive Analysis of CRISPR-Cas9 Editing Outcomes in Yeast Xanthophyllomyces dendrorhous	22
Generation of Human Isogenic Induced Pluripotent Stem Cell Lines with CRISPR Prime Editing	21
Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors	20
Specific High-Sensitivity Enzymatic Reporter UnLOCKing-Mediated Detection of Oncogenic BCR::ABL1 and EGFR Rearrangements	19
CRISPR à la carte	19

CRISPR Rewrites the Future of Medicine	18
Widespread Impact of Natural Genetic Variations in CRISPR-Cas9 Outcomes	17
A Story of Perseverance: An Interview with Matthew Porteus	17
Engineering CjCas9 for Efficient Base Editing and Prime Editing	16
Automated Good Manufacturing Practice-Compatible CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells for Clinical Treatment of β-Hemoglobinopathies	16
Combined Theoretical, Bioinformatic, and Biochemical Analyses of RNA Editing by Adenine Base Editors	14
Engineered Antiviral Sensor Targets Infected Mosquitoes	14
Rational Engineering of CRISPR-Cas9 Nuclease to Attenuate Position-Dependent Off-Target Effects	14
Evolutionary Biology and Gene Editing of Cat Allergen, Fel d 1	13
Evolution of Type IV CRISPR-Cas Systems: Insights from CRISPR Loci in Integrative Conjugative Elements of Acidithiobacillia	13
Gene Editing in the Chagas Disease Vector Rhodnius prolixus by Cas9-Mediated ReMOT Control	13
dCas9 Tells Tales: Probing Gene Function and Transcription Regulation in Cancer	13
A PCR-Induced Mutagenesis-Restriction Fragment Length Polymorphism Method for the Detection of CRISPR-Induced Indels	12
CRISPR Momentum in the Clinic and the Field	12
A Fluorescent Reporter Mouse for In Vivo Assessment of Genome Editing with Diverse Cas Nucleases and Prime Editors	11
Fanzors: Mysterious TnpB-Like Bacterial Transposon-Related RNA-Guided DNA Nucleases of Eukaryotes	11
Affordable Pricing of CRISPR Treatments is a Pressing Ethical Imperative	11
Enhancements of the CRISPR-Cas System in the Silkworm Bombyx mori	10
A Multiplexed Cas13-Based Assay with Point-of-Care Attributes for Simultaneous COVID-19 Diagnosis and Variant Surveillance	10
CRISPR Adventures in China	9
A Field Day for Gene-Edited Brassicas and Crop Improvement	9
It's a Knockout for Cat Allergies?	9
CRISPR-Cas9-Mediated Correction of TSC2 Pathogenic Variants in iPSCs from Patients with Tuberous Sclerosis Complex Type 2	9
Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines	9
A Protein-Cutting CRISPR Complex Caught in Action	9
Rapid and Technically Simple Detection of SARS-CoV-2 Variants Using CRISPR Cas12 and Cas13	9
The CRISPR Revolution in the Drug Discovery Workflow: An Industry Perspective	9
Spotlight on Genetic Design in a Spotted Wing Crop Killer	9
Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing	9
Warrior Spirit: An Interview with Victoria Gray, Sickle Cell Pioneer	9
Five Years of Progress in CRISPR Clinical Trials (2019–2024)	8
CRISPR-Cas9 Gene Editing Protects from the A53T-SNCA Overexpression-Induced Pathology of Parkinson's Disease In Vivo	8
Bacterial Retrons Enable Precise Gene Editing in Human Cells	8
Navigating Viral Space with CRISPR Technologies	8
Base Editing of Somatic Cells Using CRISPR–Cas9 in Drosophila	8
Acknowledgment of Reviewers 2023	8
Rapid Variant Pathogenicity Analysis by CRISPR Activation of CRB1 Gene Expression in Patient-Derived Fibroblasts	8
Genome Editing Therapy for the Blood: Ex Vivo Success and In Vivo Prospects	8
Special Issue: Manipulating the Microbiome with CRISPR	7
Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells	7
Genome Editing Headwinds: Can CRISPR Stay on Target?	7
Reduction of Pre-Existing Adaptive Immune Responses Against SaCas9 in Humans Using Epitope Mapping and Identification	7
Diversification of the CRISPR Toolbox: Applications of CRISPR-Cas Systems Beyond Genome Editing	6
Programming Cells by Multicopy Chromosomal Integration Using CRISPR-Associated Transposases	6
Bio-Orthogonal Chemistry Conjugation Strategy Facilitates Investigation of N-methyladenosine and Thiouridine Guide RNA Modifications on CRISPR Activity	6
Special Issue: CRISPR Trials	6
CRISPR-Cas9 Long-Read Sequencing for Mapping Transgenes in the Mouse Genome	6
Clonally Selected Lines After CRISPR-Cas Editing Are Not Isogenic	6
Extreme Genome Editing	6
Characterization of Research Support of Genome Editing Technologies and Transition to Clinical Trials	6

Diverse Class 2 CRISPR Effectors as Active Nucleases with Expanded Targeting Capabilities	6
Novel off-Targeting Events Identified after Genome Wide Analysis of CRISPR-Cas Edited Pigs	6
Correction to: Give Cas a Chance: An Actionable Path to a Platform for CRISPR Cures, by Fyodor D. Urnov [DOI: 10.1089/crispr.2024.0082]	6
Correction to: APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels by Rieffer et al. The CRISPR Journal, 2023;6(5):430–446; DOI: 10.1089/crispr.2023.0027	6
PASTE: The Way Forward for Large DNA Insertions	6
CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors	5
Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing	5
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels	5
Deep Sequencing Reveals the Comprehensive CRISPR-Cas9 Editing Spectrum in Bombyx mori	5
Celebrating a Decade of CRISPR-Fueled Genome Editing	5
An Efficient and Cost-Effective Novel Strategy for Identifying CRISPR-Cas-Mediated Mutants in Plant Offspring	5
Modified Cas9-Guided Oxford Nanopore Technology Sequencing Uncovers Single and Multiple Transgene Insertion Sites in a Zebrafish Melanoma Model	5
High-Throughput Imaging of CRISPR- and Recombinant Adeno-Associated Virus–Induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells	5
CRISPR-Cas9-Mediated Targeting of Multidrug Resistance Genes in Methicillin-Resistant Staphylococcus aureus	5
Electroporation-Mediated Delivery of Cas9 Ribonucleoproteins Results in High Levels of Gene Editing in Primary Hepatocytes	5
Viral Delivery of Compact CRISPR-Cas12f for Gene Editing Applications	5
Transversion Expansion of Base Editing	5
Optimization of Genomewide CRISPR Screens Using AsCas12a and Multi-Guide Arrays	5
Green Fluorescent Protein Tagged Polycistronic Reporter System Reveals Functional Editing Characteristics of CRISPR-Cas	4
The CRISPR Patent Landscape: Focus on Chinese Researchers	4
CRISPR Hits Home in a First-in-Human Study	4
Discovery and Characterization of Novel Type V Cas12f Nucleases with Diverse Protospacer Adjacent Motif Preferences	4
CRISPR and Chromothripsis: Proceed with Caution	4
Enhanced Myogenesis by Silencing Myostatin with Nonviral Delivery of a dCas9 Ribonucleoprotein Complex	4
Genetic Manipulation of a CAST of Characters in a Microbial Community	4
Correction to: Genotyping MUltiplexed-Sequencing of CRISPR-Localized Editing (GMUSCLE): An Experimental and Computational Approach for Analyzing CRISPR-Edited Cells by Zhang et al. The CRISP	4
Predicting Mutations Generated by Cas9, Base Editing, and Prime Editing in Mammalian Cells	4
CRISPRi-Mediated Treatment of Dominant Rhodopsin-Associated Retinitis Pigmentosa	4
AI and SynBio Meet CRISPR Heralding a New Genome Editing Era	4
Modulating CRISPR-Cas Genome Editing Using Guide-Complementary DNA Oligonucleotides	4
Efficient and Economical Targeted Insertion in Plant Genomes via Protoplast Regeneration	4
Novel and Engineered Type II CRISPR Systems from Uncultivated Microbes with Broad Genome Editing Capability	4
Identification and Evolution of Cas9 tracrRNAs	4
As CRISPR Technologies Ripen, What Should We Prioritize?	4
Developing Bottom-Up Induced Pluripotent Stem Cell Derived Solid Tumor Models Using Precision Genome Editing Technologies	4
Response to Cook et al. re: Novel Off-Targeting Events Identified After Genome Wide Analysis of CRISPR-Cas Edited Pig	4
Genomic and Transcriptomic Analyses of Prime Editing Guide RNA–Independent Off-Target Effects by Prime Editors	3
Correction to: Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas by Burger et al. The CRISPR Journal,	3
Highly Efficient One-Step Tagging of Endogenous Genes in Primary Cells Using CRISPR-Cas Ribonucleoproteins	3
CRISPRclassify: Repeat-Based Classification of CRISPR Loci	3
Special Issue on CRISPR Diagnostics	3
CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito, Aedes aegypti	3
Periodontal Disease Pathogens, Pathogenesis, and Therapeutics: The CRISPR-Cas Effect	3
Identification of a Guide RNA Targeting an Ultraconserved Element for Evaluation of Cas9 Genome Editors Across Mammalian Species	3
The Promises and Pitfalls of CRISPR-Mediated Base Editing in Stem Cells	3
Genome Editing in Apicomplexan Parasites: Current Status, Challenges, and Future Possibilities	3
Acknowledgment of Reviewers 2021	3
JACKIE: Fast Enumeration of Genome-Wide Single- and Multicopy CRISPR Target Sites and Their Off-Target Numbers	3
Fast and Efficient Generation of Isogenic Induced Pluripotent Stem Cell Lines Using Adenine Base Editing	3
Enrichment of Allelic Editing Outcomes by Prime Editing in Induced Pluripotent Stem Cells	3
CRISPR Empowers Tree Bioengineering for a Sustainable Future	2
Monitoring the Land and Sea: Enhancing Efficiency Through CRISPR-Cas Driven Depletion and Enrichment of Environmental DNA	2
Establishment of a CRISPR-Cas9-Mediated Genome Editing System in Flax	2
Efficient Generation of SOCS2 Knock-Out Sheep by Electroporation of CRISPR-Cas9 Ribonucleoprotein Complex with Dual-sgRNAs	2
Impact of KIT Editing on Coat Pigmentation and Fresh Meat Color in Yorkshire Pigs	2
Clinical Milestone for CRISPR Medicines	2
Functional and Phylogenetic Diversity of Cas10 Proteins	2
Optimizing Genome Editing in Mollusks (Crassostrea gigas) in Vitro Validation of sgRNA and Identifying Key Factors Influencing Efficiency	2
CRISPR's Path to the Clinic	2
Compact Cje3Cas9 for Efficient In Vivo Genome Editing and Adenine Base Editing	2
CRISPR and the Plant Pathologists' Holy Grail	2
Gene-Edited Fluorescent and Mixed Cerebral Organoids	2
Acknowledgment of Reviewers 2024	2
A Mutation-Agnostic Hematopoietic Stem Cell Gene Therapy for Metachromatic Leukodystrophy	2
Model System to Analyze RNA-Mediated DNA Repair in Mammalian Cells	2
Special Issue: Manipulating the Microbiome with CRISPR	2
RNA Binding Proteins As Regulators of Oxidative Stress Identified by a Targeted CRISPR-Cas9 Single Guide RNA Library	2
Functional Characterization of Type III-A CRISPR-Cas in a Clinical Human Methicillin-R Staphylococcus aureus Strain	2
Search for Origins of Anti-CRISPR Proteins by Structure Comparison	2
CLASH of the Titans: How CAR-T Cells Can Triumph Over Tumors	2
Allele-Specific Inactivation of an Autosomal Dominant Epidermolysis Bullosa Simplex Mutation Using CRISPR-Cas9	2
Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for The CRISPR Journal	2