OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-04-01 to 2024-04-01.)

Article	Citations
Microbial electro-fermentation for synthesis of chemicals and biofuels driven by bi-directional extracellular electron transfer	57
Discovery and modification of cytochrome P450 for plant natural products biosynthesis	45
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	45
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	45
Methodologies for preparation of prokaryotic extracts for cell-free expression systems	40
Challenges and advances in genome mining of ribosomally synthesized and post-translationally modified peptides (RiPPs)	40
Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast	39
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	36
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	35
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	35
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	32
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants	31
Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell	29
Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha	29
Emerging biosensors in detection of natural products	27
Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K	27
Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering	25
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	25
A high stable pH-temperature dual-sensitive liposome for tuning anticancer drug release	25
Chemical modifications of proteins and their applications in metalloenzyme studies	24
Coordinated regulation for nature products discovery and overproduction in Streptomyces	24
PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking	24
Strategies to increase tolerance and robustness of industrial microorganisms	24
Expanding application of CRISPR-Cas9 system in microorganisms	22
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	21

Melatonin biosynthesis pathways in nature and its production in engineered microorganisms	21
Mining and fine-tuning sugar uptake system for titer improvement of milbemycins in Streptomyces bingchenggensis	20
Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways	19
Complete genome sequence of high-yield strain S. lincolnensis B48 and identification of crucial mutations contributing to lincomycin overproduction	18
Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0	18
Biodegradation of aromatic pollutants meets synthetic biology	17
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	17
Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei	17
Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution	17
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	16
Synthetic polycistronic sequences in eukaryotes	16
Enhancing single-cell hyaluronic acid biosynthesis by microbial morphology engineering	16
Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways	16
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	16
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	16
Antidiabetic effect of an engineered bacterium Lactobacillus plantarum-pMG36e -GLP-1 in monkey model	15
Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort	15
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	15
Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan	15
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	14
Production of l-glutamate family amino acids in Corynebacterium glutamicum: Physiological mechanism, genetic modulation, and prospects	14
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	13
Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases	13
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	13
Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue	13
Using recombinant adhesive proteins as durable and green flame-retardant coatings	13
Metabolic engineering of Yarrowia lipolytica for scutellarin production	12
Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis	12
Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim.	12
An aurora of natural products-based drug discovery is coming	11
Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance	11
Design and construction of microbial cell factories based on systems biology	11
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	11
Comparative metagenomic analysis of the vaginal microbiome in healthy women	11
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	11
A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli	11
Positional assembly of multi-enzyme cascade reaction in polyelectrolyte doped microcapsule through electrospray and layer-by-layer assembly	11
Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering	11
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol	11
Complex natural product production methods and options	10
Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA	10
Aptamer-based cell-free detection system to detect target protein	10
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	10
Discovering the next decade's synthetic biology research trends with ChatGPT	10
Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	10
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	9
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	9
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	9
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	9
Applications of protein engineering in the microbial synthesis of plant triterpenoids	9

Application of serine integrases for secondary metabolite pathway assembly in Streptomyces	9
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	9
Physical stimuli-responsive cell-free protein synthesis	9
Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides	9
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	9