OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 10. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-06-01 to 2026-06-01.)

Article	Citations
Outside Front Cover	86
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	71
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	60
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	54
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	50
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	45
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	45
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	43
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	43
The functional analysis of NirBD in microorganisms and the efficacies of NirBD on the soil nitrogen storage and N2O emission	43
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	41
Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum	40
Degradation strategies of pesticide residue: From chemicals to synthetic biology	36
Thank you Reviewers	35
Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery	35
Synergistic engineering PETase reveals loop-region mutations for enhanced catalytic activity and thermal stability	35
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	33
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	33
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	32
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	31
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	31
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	29
Transfer learning with pre-trained language models for protein expression level prediction in Escherichia coli	29
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	29
Design and construction of microbial cell factories based on systems biology	29

Metabolic modulation of TCA cycle by S-nitrosylation in Monascus spp.	28
Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanes deccanensis	28
Regulating protein glycosylation modification enhances the synthesis of taxadiene in Saccharomyces cerevisiae	28
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	27
Engineering riboflavin-overproducing Bacillus subtilis via pathway gene overexpression	27
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	26
Recombinant production of amaranthin and other betalain variants with yeast cell factories	25
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	25
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	25
Cofactor manipulation to drive biosynthesis of natural products	24
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	23
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	23
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	23
Explore the dominant factor in prime editing via a view of DNA processing	23
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	22
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	22
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	21
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	21
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	21
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	21
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	21
Engineering non-P450 3-hydroxylase for de novo synthesizes catechol-containing compounds in Escherichia coli	20
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	20
Synthetic biotechnology for C1 bio-refinery	20
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	20
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	20
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	19
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	19
A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification	19
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	18
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	18
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	18
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	18
From consultors to collaborators – An SOP for advancing ethics engagement in science	17
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	16
Way to efficient microbial paclitaxel mass production	16
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	16
Plant metabolism and synthetic biology	16
Malic enzyme-based system for transhydrogenation between nicotinamide cofactors	16
Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects	16
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	16
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	16
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	15
Engineered dual-fluorescence functional nucleic acid-based CRISPR/Cas12a biosensor for label-free ratiometric detection of site-specific DNA methylation	15
Engineering Yarrowia lipolytica for nervonic acid production by organelle compartmentalization and dynamic promoter coordination	15
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	15
Biochemical synthesis of taxanes from mevalonate	15
Engineering an ATP-saving mevalonate pathway for high-efficiency S-(+)-linalool production in Serratia marcescens	15
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	15
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	14

Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols	14
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	14
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	14
Systems metabolic engineering of dual pathways with stage-specific activation for high-level 5-aminolevulinic acid production in Escherichia coli	14
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	14
A systematic discussion and comparison of the construction methods of synthetic microbial community	14
Applications of protein engineering in the microbial synthesis of plant triterpenoids	14
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	14
Topoisomerase II-Like variants enhance acid tolerance and low-pH l-malic acid biosynthesis in metabolic engineered Aspergillus nidulans	14
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	14
Engineering Rubisco to enhance CO2 utilization	14
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	13
CO2-elevated cell-free protein synthesis	13
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	13
DNA storage: The future direction for medical cold data storage	13
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	13
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	13
Programmable large-cargo integration: Overcoming size constraints for next-generation gene therapy	12
Synthetic biology techniques to tackle heavy metal pollution and poisoning	12
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	12
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	12
Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis	12
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	11
Single carbon metabolism – A new paradigm for microbial bioprocesses?	11
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	11
De novo biosynthesis of Asperosaponin VI in Saccharomyces cerevisiae	11
Decoupled tricarboxylic acid cycle and glycolysis of Corynebacterium glutamicum combined with acetate supply promote high-yield biosynthesis of l-homoserine	11
Simulating androgen receptor selection in designer yeast	11
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	11
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	11
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	11
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	11
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	11
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	11
Engineered microbes: Making valuable chemicals from waste gases	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	11
Developing safe and efficient CGBE editor based on Cas-embedding strategy	11
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	11
Biotransformation of ethylene glycol by engineered Escherichia coli	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases	10
Development and application of synthetic biology tools for improved production of human breast milk components	10
Advances in stress-tolerance elements for microbial cell factories	10
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
Engineering ω-transaminase for efficient dihydroxyacetone transamination in serinol biosynthesis starting from methanol	10
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	10
Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library	10
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	10
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
Functions and optimization of soft law in the international governance of synthetic biology: The predicament of hard law vs. the rise of soft law	10
Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy	10
Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells	10
Systematic design and evaluation of artificial CO2 assimilation pathways	10