OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-10-01 to 2025-10-01.)

Article	Citations
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	85
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	73
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	56
Outside Front Cover	56
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	51
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	44
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	41
Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum	41
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	40
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	39
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	38
Degradation strategies of pesticide residue: From chemicals to synthetic biology	38
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	34
Design and construction of microbial cell factories based on systems biology	34
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	33
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	32
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	31
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	30
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	30
Thank you Reviewers	28
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	28
Research progress of pathway and genome evolution in microbes	28
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	27
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	27
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	26

Cofactor manipulation to drive biosynthesis of natural products	25
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	25
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	25
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	25
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	25
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	23
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	23
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	22
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	22
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	22
Explore the dominant factor in prime editing via a view of DNA processing	22
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	22
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	21
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	21
Synthetic biotechnology for C1 bio-refinery	21
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	21
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	19
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	19
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	19
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	19
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	18
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	18
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	18
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	18
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	18
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	18
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	17
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	17
Plant metabolism and synthetic biology	17
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	17
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	17
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	17
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	17
Way to efficient microbial paclitaxel mass production	16
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	16
Engineering Rubisco to enhance CO2 utilization	16
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	16
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	16
Biochemical synthesis of taxanes from mevalonate	16
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	16
Applications of protein engineering in the microbial synthesis of plant triterpenoids	14
A systematic discussion and comparison of the construction methods of synthetic microbial community	14
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	14
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	14
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	14
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	14
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	13
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	13
CO2-elevated cell-free protein synthesis	13
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	13

Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	13
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	13
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	12
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	12
DNA storage: The future direction for medical cold data storage	12
Single carbon metabolism – A new paradigm for microbial bioprocesses?	12
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	12
Engineered microbes: Making valuable chemicals from waste gases	11
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
Simulating androgen receptor selection in designer yeast	11
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	11
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	11
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	11
Synthetic biology techniques to tackle heavy metal pollution and poisoning	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	10
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	10
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	10
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	10
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	10
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	10
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	10
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
Advances in stress-tolerance elements for microbial cell factories	9
Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases	9
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	9
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	9
Biotransformation of ethylene glycol by engineered Escherichia coli	9
Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy	9
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	9
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	9
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	9
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	9
Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells	9
Developing safe and efficient CGBE editor based on Cas-embedding strategy	9
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	9