OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 10. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-01-01 to 2026-01-01.)

Article	Citations
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	94
Outside Front Cover	76
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	66
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	64
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	57
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	52
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	47
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	43
Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery	43
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	39
Degradation strategies of pesticide residue: From chemicals to synthetic biology	38
Thank you Reviewers	35
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	35
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	34
Research progress of pathway and genome evolution in microbes	33
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	32
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	30
Design and construction of microbial cell factories based on systems biology	30
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	30
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	30
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	30
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	30
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	30
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	28
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	28

Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanes deccanensis	27
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	26
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	26
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	25
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	25
Cofactor manipulation to drive biosynthesis of natural products	25
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	24
Explore the dominant factor in prime editing via a view of DNA processing	24
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	24
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	24
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	23
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	23
Recombinant production of amaranthin and other betalain variants with yeast cell factories	23
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	22
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	22
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	21
Synthetic biotechnology for C1 bio-refinery	20
Engineering non-P450 3-hydroxylase for de novo synthesizes catechol-containing compounds in Escherichia coli	19
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	19
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	19
A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification	19
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	19
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	18
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	18
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	18
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	18
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	18
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	18
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	17
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	17
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	17
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	17
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	16
Plant metabolism and synthetic biology	16
From consultors to collaborators – An SOP for advancing ethics engagement in science	15
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	15
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	15
Way to efficient microbial paclitaxel mass production	15
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	15
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	15
Biochemical synthesis of taxanes from mevalonate	14
Applications of protein engineering in the microbial synthesis of plant triterpenoids	13
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	13
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	13
A systematic discussion and comparison of the construction methods of synthetic microbial community	13
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	13
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	13
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	13
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	13
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	13

Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	13
Engineering Rubisco to enhance CO2 utilization	13
DNA storage: The future direction for medical cold data storage	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	12
Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis	12
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	12
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	12
Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols	12
CO2-elevated cell-free protein synthesis	12
Single carbon metabolism – A new paradigm for microbial bioprocesses?	12
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	12
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	12
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	11
Engineered microbes: Making valuable chemicals from waste gases	11
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	11
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	11
Synthetic biology techniques to tackle heavy metal pollution and poisoning	11
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	11
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	11
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	11
Simulating androgen receptor selection in designer yeast	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	11
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	10
Developing safe and efficient CGBE editor based on Cas-embedding strategy	10
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	10
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	10
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	10