OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-04-01 to 2025-04-01.)

Article	Citations
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	70
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	68
Origin and evolution of the main starch biosynthetic enzymes	59
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	55
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	51
Thank you Reviewers list	44
Thank you Reviewers list	43
CO2-elevated cell-free protein synthesis	41
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	38
Gaining access to acetyl-CoA by peroxisomal surface display	37
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	36
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	36
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	35
H-NS family protein MvaU downregulates phenazine-1-carboxylic acid (PCA) biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas st	35
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	32
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	30
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	29
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	29
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	29
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	29
Production of N-acetylglucosamine with Vibrio alginolyticus FA2, an emerging platform for economical unsterile open fermentation	28
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	27
The potential of plant proteins as antifungal agents for agricultural applications	27
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	25
Omics data analysis reveals the system-level constraint on cellular amino acid composition	24

Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production	23
Development of Zn2+-controlled expression system for lactic acid bacteria and its application in engineered probiotics	23
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	22
Engineering artificial cross-species promoters with different transcriptional strengths	22
Discovering peptides and computational investigations of a multiepitope vaccine target Mycobacterium tuberculosis	22
Developing polycistronic expression tool in Yarrowia lipolytica	22
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	21
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	21
A generative benchmark for evaluating the performance of fluorescent cell image segmentation	20
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	20
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	20
Outside Front Cover	20
Optimized dual-AAV base editor delivery system with enhanced editing efficiency and virion production titer	19
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	19
Functional characterization of triterpene synthases in Cibotium barometz	19
DNA Storage: The Future Direction for Medical Cold Data Storage	19
Simultaneous multiplex genome loci editing of Halomonas bluephagenesis using an engineered CRISPR-guided base editor	18
A toolbox for genetic manipulation in intestinal Clostridium symbiosum	17
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	17
Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases	16
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	16
Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	16
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	15
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	15
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	15
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	15
Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE	15
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	15
Strategies to increase tolerance and robustness of industrial microorganisms	14
Single carbon metabolism – A new paradigm for microbial bioprocesses?	14
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	14
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	14
Advance of tolerance engineering on microbes for industrial production	14
Synthetic biology techniques to tackle heavy metal pollution and poisoning	14
Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae	14
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	14
Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris	13
Degradation strategies of pesticide residue: From chemicals to synthetic biology	13
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	13
Engineered microorganisms: A new direction in kidney stone prevention and treatment	13
Functional investigation of the SAM-dependent methyltransferase RdmB in anthracycline biosynthesis	12
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	12
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	12
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	12
Fructose promotes pyoluteorin biosynthesis via the CbrAB-CrcZ-Hfq/Crc pathway in the biocontrol strain Pseudomonas PA1201	12
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	12
New N-acylated aminoalkanoic acids from tea roots derived biocontrol agent Clonostachys rosea 15020	12
Editorial for “Special Issue on the 2019 and 2020 iGEM proceedings”	12
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	11
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	11

Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi	11
Intelligent tunable CAR-T cell therapy leads the new trend	11
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	11
Biosensors for the detection of flaviviruses: A review	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	11
Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses	10
Combined strategies for improving the heterologous expression of a novel xylanase from Fusarium oxysporum Fo47 in Pichia pastoris	10
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	10
Regulation and management of the biosecurity for synthetic biology	9
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	9
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	9
Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette of Vitreoscilla hemoglobin in Bacillus licheniformis	9
Turning waste gases into valuables	9
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	9
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	9
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	9
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	9