OOIR: Observatory of International Research

Papers

(The TQCC of Synthetic and Systems Biotechnology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-12-01 to 2024-12-01.)

Article	Citations
Microbial electro-fermentation for synthesis of chemicals and biofuels driven by bi-directional extracellular electron transfer	65
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	56
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	55
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	51
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	46
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	45
Methodologies for preparation of prokaryotic extracts for cell-free expression systems	43
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	41
Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering	36
Melatonin biosynthesis pathways in nature and its production in engineered microorganisms	34
Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K	34
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants	34
Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha	33
PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking	32
Emerging biosensors in detection of natural products	31
Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell	30
Chemical modifications of proteins and their applications in metalloenzyme studies	30
Strategies to increase tolerance and robustness of industrial microorganisms	30
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	29
Using recombinant adhesive proteins as durable and green flame-retardant coatings	28
Expanding application of CRISPR-Cas9 system in microorganisms	28
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	28
Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways	25
Biodegradation of aromatic pollutants meets synthetic biology	24
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	23

Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	22
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	22
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	22
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	21
Production of l-glutamate family amino acids in Corynebacterium glutamicum: Physiological mechanism, genetic modulation, and prospects	20
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	20
Enhancing single-cell hyaluronic acid biosynthesis by microbial morphology engineering	19
Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution	18
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	18
Degradation strategies of pesticide residue: From chemicals to synthetic biology	18
Synthetic polycistronic sequences in eukaryotes	18
The potential of plant proteins as antifungal agents for agricultural applications	18
Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim.	17
Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs	17
Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan	17
Antidiabetic effect of an engineered bacterium Lactobacillus plantarum-pMG36e -GLP-1 in monkey model	16
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	16
Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA	15
Synthetic biology techniques to tackle heavy metal pollution and poisoning	15
Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue	15
Metabolic engineering of Yarrowia lipolytica for scutellarin production	14
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol	14
Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis	14
Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	14
Comparative metagenomic analysis of the vaginal microbiome in healthy women	14
Discovering the next decade's synthetic biology research trends with ChatGPT	14
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	13
Physical stimuli-responsive cell-free protein synthesis	13
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	13
Microbial soluble aromatic prenyltransferases for engineered biosynthesis	13
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	13
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	12
Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering	12
A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli	12
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	12
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	12
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	12
Design and construction of microbial cell factories based on systems biology	12
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	12
Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance	12
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	12
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	12
Ameliorating end-product inhibition to improve cadaverine production in engineered Escherichia coli and its application in the synthesis of bio-based diisocyanates	11
Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides	11
Complex natural product production methods and options	11
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	11
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	11
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	11
Applications of protein engineering in the microbial synthesis of plant triterpenoids	10
Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans	10

O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	10
Aptamer-based cell-free detection system to detect target protein	10
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	10
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	10
Mechanisms and biotechnological applications of transcription factors	10
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	10
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	10
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	9
Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects in Saccharomyces cerevisiae	9
Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette of Vitreoscilla hemoglobin in Bacillus licheniformis	9
Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol	9
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	9
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	9
Boosted activity by engineering the enzyme microenvironment in cascade reaction: A molecular understanding	9
Structure-based identification and pathway elucidation of flavonoids in Camptotheca acuminate	9