Synthetic and Systems Biotechnology

Article	Citations
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	75
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	70
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	62
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	56
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	54
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	46
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	44
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	43
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	40
Research progress of pathway and genome evolution in microbes	37
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	37
Degradation strategies of pesticide residue: From chemicals to synthetic biology	37
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	36
Thank you Reviewers	35
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	33
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	31
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	31
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	31
Design and construction of microbial cell factories based on systems biology	30
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	29
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	29
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	27
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	27
Cofactor manipulation to drive biosynthesis of natural products	26
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	26

Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	25
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	25
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	24
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	23
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	23
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	23
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	22
Explore the dominant factor in prime editing via a view of DNA processing	22
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	21
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	21
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	21
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	21
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	21
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	20
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	19
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	19
Synthetic biotechnology for C1 bio-refinery	19
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	18
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	18
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	17
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	17
Plant metabolism and synthetic biology	17
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	17
Way to efficient microbial paclitaxel mass production	17
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	16
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	16
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	16
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	16
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	16
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	15
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	15
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	15
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	15
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	15
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	15
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	14
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	14
PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking	14
A systematic discussion and comparison of the construction methods of synthetic microbial community	14
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	14
Aptamer-based cell-free detection system to detect target protein	14
Biochemical synthesis of taxanes from mevalonate	14
Applications of protein engineering in the microbial synthesis of plant triterpenoids	14
Engineering Rubisco to enhance CO2 utilization	14
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	13
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	13
Synthetic biology techniques to tackle heavy metal pollution and poisoning	13
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	13
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	13
CO2-elevated cell-free protein synthesis	13

Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	13
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	12
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	12
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	11
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	11
Single carbon metabolism – A new paradigm for microbial bioprocesses?	11
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	11
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	11
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	10
Engineered microbes: Making valuable chemicals from waste gases	10
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	10
Simulating androgen receptor selection in designer yeast	10
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	9
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	9
Biotransformation of ethylene glycol by engineered Escherichia coli	9
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	9
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	9
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	9
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	9
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	9
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	9
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	9
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction	8
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	8
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	8
Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad in Saccharopolyspora spinosa	8
Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression	8
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	8
Advances in stress-tolerance elements for microbial cell factories	8
Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via the β-alanine pathway	8
Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae	8
Deacetoxycephalosporin C synthase (expandase): Research progress and application potential	8
Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae	8
Engineered probiotic-mediated intratumoral delivery and controlled release of bacterial collagenase for cancer therapy	8
Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis	7
Corrigendum to “Improving cooperativity of transcription activators by oligomerization domains in mammalian cells” [Synth Syst Biotechnol 8 (1) (2023) 114–120]	7
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	7
Outside Front Cover	7
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	7
An efficient marker recycling system for sequential gene deletion in a deep sea-derived fungus Acremonium sp. HDN16-126	7
Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology	7
Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling	7
Thank you Reviewers list	7
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	7
Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in Saccharomyces cerevisiae	7
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917	7
Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments	7
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	7
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	7
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	7
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	6
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	6
Efficient fatty acid synthesis from methanol in methylotrophic yeast	6
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	6
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	6
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	6
Study on RNAi-based herbicide for Mikania micrantha	6
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	6
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	6
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	6
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	6
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	6
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	6
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	6
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	6
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	6
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	6
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	5
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	5
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	5
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	5
Discovering the next decade's synthetic biology research trends with ChatGPT	5
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	5
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	5
Network of nicotinamide mononucleotide biosynthesis: substrate tracking, rate-limiting enzyme, and regulatory strategy	5

Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	5
Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities	5
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	5
Construction of microbial chassis for terpenoid discovery	5
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	5
Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket	5
Design and mathematical analysis of activating transcriptional amplifiers that enable modular temporal control in synthetic juxtacrine circuits	5
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	5
Functional characterization of triterpene synthases in Cibotium barometz	4
Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilized Saccharomyces cerevisiae cells	4
Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst	4
Origin and evolution of the main starch biosynthetic enzymes	4
Advance of tolerance engineering on microbes for industrial production	4
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	4
Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum	4
Transcriptomic Studies on the Product Stress Response Revealed that YCF1 is a Beneficial Factor for Progesterone Production in Yarrowia lipolytica	4
Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin	4
The potential of plant proteins as antifungal agents for agricultural applications	4
Mechanisms and biotechnological applications of transcription factors	4
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	4
Thank you Reviewers	4
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	4
Outside Front Cover	4
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	4
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	4
Engineered microorganisms: A new direction in kidney stone prevention and treatment	4
Improving cooperativity of transcription activators by oligomerization domains in mammalian cells	4
Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production	4
Enhancing levan biosynthesis by destroying the strongly acidic environment caused by membrane-bound glucose dehydrogenase (mGDH) in Gluconobacter sp. MP2116	4
H-NS family protein MvaU downregulates phenazine-1-carboxylic acid (PCA) biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas st	4
Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering	4
Editorial for “Biointelligent manufacturing in Memorium of Arnold (Arny) L. Demain”	4
Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase	4
Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii	4
Engineering artificial cross-species promoters with different transcriptional strengths	4
finDr: A web server for in silico D-peptide ligand identification	4
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	4
Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways	4
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	4
Comparative metagenomic analysis of the vaginal microbiome in healthy women	4