Synthetic and Systems Biotechnology

Article	Citations
Microbial electro-fermentation for synthesis of chemicals and biofuels driven by bi-directional extracellular electron transfer	65
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	55
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	51
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	46
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	46
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	45
Methodologies for preparation of prokaryotic extracts for cell-free expression systems	43
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	41
Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K	34
Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering	34
Melatonin biosynthesis pathways in nature and its production in engineered microorganisms	34
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants	34
Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha	33
Emerging biosensors in detection of natural products	31
Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell	30
Chemical modifications of proteins and their applications in metalloenzyme studies	30
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	29
PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking	29
Expanding application of CRISPR-Cas9 system in microorganisms	28
Strategies to increase tolerance and robustness of industrial microorganisms	27
Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways	23
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	23
Using recombinant adhesive proteins as durable and green flame-retardant coatings	22
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	22
Biodegradation of aromatic pollutants meets synthetic biology	22

Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	22
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	21
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	21
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	21
Production of l-glutamate family amino acids in Corynebacterium glutamicum: Physiological mechanism, genetic modulation, and prospects	20
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	20
Enhancing single-cell hyaluronic acid biosynthesis by microbial morphology engineering	19
Synthetic polycistronic sequences in eukaryotes	18
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	18
The potential of plant proteins as antifungal agents for agricultural applications	18
Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution	18
Degradation strategies of pesticide residue: From chemicals to synthetic biology	18
Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan	17
Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim.	17
Antidiabetic effect of an engineered bacterium Lactobacillus plantarum-pMG36e -GLP-1 in monkey model	16
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	16
Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue	15
Synthetic biology techniques to tackle heavy metal pollution and poisoning	15
Comparative metagenomic analysis of the vaginal microbiome in healthy women	14
Discovering the next decade's synthetic biology research trends with ChatGPT	14
Metabolic engineering of Yarrowia lipolytica for scutellarin production	14
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol	14
Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis	14
Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA	14
Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs	13
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	13
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	13
Physical stimuli-responsive cell-free protein synthesis	13
Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	13
Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance	12
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	12
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	12
Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering	12
Design and construction of microbial cell factories based on systems biology	12
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	12
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	12
A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli	12
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	11
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	11
Ameliorating end-product inhibition to improve cadaverine production in engineered Escherichia coli and its application in the synthesis of bio-based diisocyanates	11
Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides	11
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	11
Complex natural product production methods and options	11
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	11
Mechanisms and biotechnological applications of transcription factors	10
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	10
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	10
Applications of protein engineering in the microbial synthesis of plant triterpenoids	10
Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans	10
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	10

Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	10
Aptamer-based cell-free detection system to detect target protein	10
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	10
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	10
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	10
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	9
Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects in Saccharomyces cerevisiae	9
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	9
Boosted activity by engineering the enzyme microenvironment in cascade reaction: A molecular understanding	9
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	9
Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol	9
Structure-based identification and pathway elucidation of flavonoids in Camptotheca acuminate	9
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	9
Identification and heterologous expression of the globomycin biosynthetic gene cluster	8
The ornithine-urea cycle involves fumaric acid biosynthesis in Aureobasidium pullulans var. aubasidani, a green and eco-friendly process for fumaric acid production	8
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	8
Functional characterization of key polyketide synthases by integrated metabolome and transcriptome analysis on curcuminoid biosynthesis in Curcuma wenyujin	8
Microbial soluble aromatic prenyltransferases for engineered biosynthesis	8
Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis	8
Expression, secretion and functional characterization of three laccases in E. coli	8
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	8
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	8
Study on RNAi-based herbicide for Mikania micrantha	8
Engineering microbes to overproduce natural products as agrochemicals	8
Enhanced cobalamin biosynthesis in Ensifer adhaerens by regulation of key genes with gradient promoters	7
The Fold-Illuminator: A low-cost, portable, and disposable incubator-illuminator device	7
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	7
Editorial for special issue on green biomanufacturing	7
Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces	7
Enhancement of phycocyanobilin biosynthesis in Escherichia coli by strengthening the supply of precursor and artificially self-assembly complex	7
Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette of Vitreoscilla hemoglobin in Bacillus licheniformis	7
m4C DNA methylation regulates biosynthesis of daptomycin in Streptomyces roseosporus L30	6
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	6
Genomic scanning enabling discovery of a new antibacterial bicyclic carbamate-containing alkaloid	6
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	6
Inhibitor tolerance and bioethanol fermentability of levoglucosan-utilizing Escherichia coli were enhanced by overexpression of stress-responsive gene ycfR: The proteomics-guided metabolic engineering	6
Expression of fungal biosynthetic gene clusters in S. cerevisiae for natural product discovery	6
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	6
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	6
Engineering Saccharomyces cerevisiae for enhanced (–)-α-bisabolol production	6
Enzymatic synthesis of indigo derivatives by tuning P450 BM3 peroxygenases	6
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	6
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	6
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	5
Applications of phage-derived RNA-based technologies in synthetic biology	5
A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain	5
Engineering Rubisco to enhance CO2 utilization	5
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	5
Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function	5
Regulation and management of the biosecurity for synthetic biology	5
finDr: A web server for in silico D-peptide ligand identification	5
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	5
Deacetoxycephalosporin C synthase (expandase): Research progress and application potential	5
Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli	5
Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE	5
Promoter engineering enables precise metabolic regulation towards efficient β-elemene production in Ogataea polymorpha	5
Engineering a cell-penetrating hyperstable antibody scFv(Ras) – An extraordinary approach to cancer therapeutics	4
Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis	4
Highly efficient multiplex base editing: One-shot deactivation of eight genes in Shewanella oneidensis MR-1	4
Good and bad dispositions between archaea and bacteria in the human gut: New insights from metagenomic survey and co-occurrence analysis	4
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	4
Research progress of pathway and genome evolution in microbes	4
Efficient fatty acid synthesis from methanol in methylotrophic yeast	4
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	4
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	4
A linear DNA template-based framework for site-specific unnatural amino acid incorporation	4
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	4
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	4
Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier	4
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	4
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	4
Synthesis and assembly of full-length cyanophage A-4L genome	4
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	4
An efficient marker recycling system for sequential gene deletion in a deep sea-derived fungus Acremonium sp. HDN16-126	4
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917	4
High-throughput and reliable acquisition of in vivo turnover number fuels precise metabolic engineering	4
Heparin mimetics as potential intervention for COVID-19 and their bio-manufacturing	4
De novo biosynthesis of a vinblastine precursor in Pichia pastoris	4
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	4
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	4

Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1	4
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	4
An improved CRISPRi system in Pichia pastoris	3
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	3
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	3
Engineered geranyl diphosphate methyltransferase produces 2-methyl-dimethylallyl diphosphate as a noncanonical C6 unit for terpenoid biosynthesis	3
Design of artificial small regulatory trans-RNA for gene knockdown in Bacillus subtilis	3
Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system	3
Simulating androgen receptor selection in designer yeast	3
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	3
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	3
Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum	3
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	3
Professor Arnold L. (“Arny”) Demain ( 1927-2020): leaving a legacy in the field of Industrial Microbiology and Biotechnology ; a personal appraisal.	3
Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities	3
Formate as a supplementary substrate facilitates sugar metabolism and solvent production by Clostridium beijerinckii NCIMB 8052	3
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	3
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	3
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	3
CO2-elevated cell-free protein synthesis	3
Computational prediction and validation of specific EmbR binding site on PknH	3
Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde	3
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	3
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	3
Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system	3
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	3