Synthetic and Systems Biotechnology

Article	Citations
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	82
Outside Front Cover	72
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	62
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	55
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	52
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	51
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	49
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	45
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	42
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	41
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	40
Thank you Reviewers	38
Degradation strategies of pesticide residue: From chemicals to synthetic biology	38
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	35
Design and construction of microbial cell factories based on systems biology	34
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	34
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	33
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	31
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	31
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	29
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	29
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	29
Research progress of pathway and genome evolution in microbes	28
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	28
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	27

Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	27
Cofactor manipulation to drive biosynthesis of natural products	27
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	27
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	26
Explore the dominant factor in prime editing via a view of DNA processing	26
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	24
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	24
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	24
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	22
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	22
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	22
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	22
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	22
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	22
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	21
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	21
Synthetic biotechnology for C1 bio-refinery	21
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	21
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	21
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	21
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	19
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	18
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	18
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	18
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	18
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	17
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	17
Malic enzyme-based system for transhydrogenation between nicotinamide cofactors	17
Way to efficient microbial paclitaxel mass production	17
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	17
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	17
Plant metabolism and synthetic biology	17
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	17
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	17
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	16
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	16
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	16
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	16
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	15
Aptamer-based cell-free detection system to detect target protein	15
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	15
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	15
Applications of protein engineering in the microbial synthesis of plant triterpenoids	15
Biochemical synthesis of taxanes from mevalonate	15
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	15
A systematic discussion and comparison of the construction methods of synthetic microbial community	14
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	14
Engineering Rubisco to enhance CO2 utilization	14
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	14
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	14

Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	13
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	13
Synthetic biology techniques to tackle heavy metal pollution and poisoning	13
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	13
CO2-elevated cell-free protein synthesis	13
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	13
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	12
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	12
Single carbon metabolism – A new paradigm for microbial bioprocesses?	12
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	11
Simulating androgen receptor selection in designer yeast	11
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	11
DNA storage: The future direction for medical cold data storage	11
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	11
Topoisomerase II-Like variants enhance acid tolerance and low-pH L-malic acid biosynthesis in metabolic engineered Aspergillus nidulans	11
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	11
Engineered microbes: Making valuable chemicals from waste gases	11
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	11
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	10
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	10
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	10
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	10
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	10
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	10
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	10
Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells	9
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	9
Deacetoxycephalosporin C synthase (expandase): Research progress and application potential	9
Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases	9
Biotransformation of ethylene glycol by engineered Escherichia coli	9
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	9
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	9
Developing safe and efficient CGBE editor based on Cas-embedding strategy	9
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	9
Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy	9
Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae	8
CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae	8
Engineered probiotic-mediated intratumoral delivery and controlled release of bacterial collagenase for cancer therapy	8
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering	8
Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression	8
Rapid detection of genotoxicity employing an engineered high-sensitivity and tight SOS-response biosensor in Escherichia coli MG1655	8
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction	8
Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae	8
Advances in stress-tolerance elements for microbial cell factories	8
Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad in Saccharopolyspora spinosa	8
Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library	8
Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via the β-alanine pathway	8
Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in Saccharomyces cerevisiae	7
Corrigendum to “Improving cooperativity of transcription activators by oligomerization domains in mammalian cells” [Synth Syst Biotechnol 8 (1) (2023) 114–120]	7
Outside Front Cover	7
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	7
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	7
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	7
Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology	7
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	7
Thank you Reviewers list	7
Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments	7
Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling	7
Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis	7
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917	7
A new paradigm for the regulation of A40926B0 biosynthesis	7
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	7
High-efficiency patatin expression strategies in Komagataella phaffii (Pichia pastoris): Expression cassette toolbox and regulation of protein secretion	7
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	6
Efficient fatty acid synthesis from methanol in methylotrophic yeast	6
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	6
A two-stage metabolome refining pipeline for natural products discovery	6
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	6
Construction of microbial chassis for terpenoid discovery	6
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	6

Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	6
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	6
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	6
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	6
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris	6
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	6
Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae	6
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	6
Study on RNAi-based herbicide for Mikania micrantha	6
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production	6
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	6
System-level characterization of engineered and evolved formatotrophic E. coli strains	6
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	6
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	6
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	6
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	6
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	6
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	6
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	6
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	5
Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica	5
Design and mathematical analysis of activating transcriptional amplifiers that enable modular temporal control in synthetic juxtacrine circuits	5
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	5
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	5
Harnessing synthetic biology for astaxanthin production: Recent advances and challenges	5
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	5
iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters	5
Polyhydroxyalkanoate production during electroactive biofilm formation and stabilization in wetland microbial fuel cells for petroleum hydrocarbon bioconversion	5
Network of nicotinamide mononucleotide biosynthesis: substrate tracking, rate-limiting enzyme, and regulatory strategy	5
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	5
Modulating ion channels with nanobodies	5
High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast	5
Discovering the next decade's synthetic biology research trends with ChatGPT	5
Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities	5
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	5
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	5
Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket	5
T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis	5
Editorial for “Biointelligent manufacturing in Memorium of Arnold (Arny) L. Demain”	4
Thank you Reviewers	4
Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering	4
Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum	4
Efficient production of hydroxysalidroside in Escherichia coli via enhanced glycosylation and semi-rational design of UGT85A1	4
Outside Front Cover	4
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	4
Engineering artificial cross-species promoters with different transcriptional strengths	4
Engineering the cytochrome P450 to enhance parthenolide production in Saccharomyces cerevisiae	4
Mechanisms and biotechnological applications of transcription factors	4
Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilized Saccharomyces cerevisiae cells	4
Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst	4
Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase	4
Engineered microorganisms: A new direction in kidney stone prevention and treatment	4
Origin and evolution of the main starch biosynthetic enzymes	4
The potential of plant proteins as antifungal agents for agricultural applications	4
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	4
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	4
Improving cooperativity of transcription activators by oligomerization domains in mammalian cells	4
Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin	4
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	4
finDr: A web server for in silico D-peptide ligand identification	4
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	4
Advance of tolerance engineering on microbes for industrial production	4
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	4
Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii	4
H-NS family protein MvaU downregulates phenazine-1-carboxylic acid (PCA) biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas st	4
dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolyspora erythraea	4
Enhancing levan biosynthesis by destroying the strongly acidic environment caused by membrane-bound glucose dehydrogenase (mGDH) in Gluconobacter sp. MP2116	4