Synthetic and Systems Biotechnology

Article	Citations
Biofilm architecture: An emerging synthetic biology target	65
Microbial electro-fermentation for synthesis of chemicals and biofuels driven by bi-directional extracellular electron transfer	56
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	45
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	45
Discovery and modification of cytochrome P450 for plant natural products biosynthesis	45
Methodologies for preparation of prokaryotic extracts for cell-free expression systems	39
Challenges and advances in genome mining of ribosomally synthesized and post-translationally modified peptides (RiPPs)	39
Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast	38
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	33
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	33
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	32
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	31
Designing chimeric enzymes inspired by fungal cellulosomes	31
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants	30
Advances on systems metabolic engineering of Bacillus subtilis as a chassis cell	29
Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha	28
Emerging biosensors in detection of natural products	25
Systematic optimization of the yeast cell factory for sustainable and high efficiency production of bioactive ginsenoside compound K	25
Complex peptide natural products: Biosynthetic principles, challenges and opportunities for pathway engineering	24
A high stable pH-temperature dual-sensitive liposome for tuning anticancer drug release	24
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	24
Coordinated regulation for nature products discovery and overproduction in Streptomyces	24
Chemical modifications of proteins and their applications in metalloenzyme studies	23
Strategies to increase tolerance and robustness of industrial microorganisms	22
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	21

PCPD: Plant cytochrome P450 database and web-based tools for structural construction and ligand docking	21
Melatonin biosynthesis pathways in nature and its production in engineered microorganisms	21
Expanding application of CRISPR-Cas9 system in microorganisms	20
Mining and fine-tuning sugar uptake system for titer improvement of milbemycins in Streptomyces bingchenggensis	20
Rationally optimized generation of integrated Escherichia coli with stable and high yield lycopene biosynthesis from heterologous mevalonate (MVA) and lycopene expression pathways	19
Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0	18
Biodegradation of aromatic pollutants meets synthetic biology	17
Complete genome sequence of high-yield strain S. lincolnensis B48 and identification of crucial mutations contributing to lincomycin overproduction	17
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	16
Enhancing single-cell hyaluronic acid biosynthesis by microbial morphology engineering	16
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	16
Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution	16
Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei	16
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	16
Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways	16
Synthetic polycistronic sequences in eukaryotes	15
Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort	15
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	15
Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan	15
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	14
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	14
Antidiabetic effect of an engineered bacterium Lactobacillus plantarum-pMG36e -GLP-1 in monkey model	14
Using recombinant adhesive proteins as durable and green flame-retardant coatings	13
Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast	13
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	13
Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases	13
Production of l-glutamate family amino acids in Corynebacterium glutamicum: Physiological mechanism, genetic modulation, and prospects	13
Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue	13
Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim.	12
Construction of synthetic microbial consortia for 2-keto-L-gulonic acid biosynthesis	12
Comparative metagenomic analysis of the vaginal microbiome in healthy women	11
An aurora of natural products-based drug discovery is coming	11
Production of (2S)-sakuranetin from (2S)-naringenin in Escherichia coli by strengthening methylation process and cell resistance	11
Positional assembly of multi-enzyme cascade reaction in polyelectrolyte doped microcapsule through electrospray and layer-by-layer assembly	11
Metabolic engineering of Yarrowia lipolytica for scutellarin production	11
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	11
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	10
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol	10
Complex natural product production methods and options	10
Aptamer-based cell-free detection system to detect target protein	9
Discovering the next decade's synthetic biology research trends with ChatGPT	9
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	9
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	9
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	9
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	9
Application of serine integrases for secondary metabolite pathway assembly in Streptomyces	9
A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli	9
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	9
Applications of protein engineering in the microbial synthesis of plant triterpenoids	9
Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides	9

Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	9
Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA	9
Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol	8
Enhanced production of l-sorbose by systematic engineering of dehydrogenases in Gluconobacter oxydans	8
Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis	8
Physical stimuli-responsive cell-free protein synthesis	8
Toward fine-tuned metabolic networks in industrial microorganisms	8
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	8
Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering	8
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	8
Degradation strategies of pesticide residue: From chemicals to synthetic biology	8
From nature to nurture: Essence and methods to isolate robust methanotrophic bacteria	7
Silk fibroin as an additive for cell-free protein synthesis	7
Design and construction of microbial cell factories based on systems biology	7
Study on RNAi-based herbicide for Mikania micrantha	7
Ameliorating end-product inhibition to improve cadaverine production in engineered Escherichia coli and its application in the synthesis of bio-based diisocyanates	7
Microbial soluble aromatic prenyltransferases for engineered biosynthesis	7
Synthetic biology techniques to tackle heavy metal pollution and poisoning	7
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	7
Cell-free synthesis system-assisted pathway bottleneck diagnosis and engineering in Bacillus subtilis	7
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	7
Boosted activity by engineering the enzyme microenvironment in cascade reaction: A molecular understanding	6
Expression of fungal biosynthetic gene clusters in S. cerevisiae for natural product discovery	6
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	6
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	6
Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces	6
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	6
Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs	6
Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects in Saccharomyces cerevisiae	6
Functional characterization of key polyketide synthases by integrated metabolome and transcriptome analysis on curcuminoid biosynthesis in Curcuma wenyujin	6
The Fold-Illuminator: A low-cost, portable, and disposable incubator-illuminator device	6
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	6
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	6
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	6
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	6
Prudently conduct the engineering and synthesis of the SARS-CoV-2 virus	6
Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette of Vitreoscilla hemoglobin in Bacillus licheniformis	6
The potential of plant proteins as antifungal agents for agricultural applications	6
Expression, secretion and functional characterization of three laccases in E. coli	5
Genomic scanning enabling discovery of a new antibacterial bicyclic carbamate-containing alkaloid	5
Applications of phage-derived RNA-based technologies in synthetic biology	5
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	5
Regulation and management of the biosecurity for synthetic biology	5
Inhibitor tolerance and bioethanol fermentability of levoglucosan-utilizing Escherichia coli were enhanced by overexpression of stress-responsive gene ycfR: The proteomics-guided metabolic engineering	5
Deacetoxycephalosporin C synthase (expandase): Research progress and application potential	5
Identification and heterologous expression of the globomycin biosynthetic gene cluster	5
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	5
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	5
finDr: A web server for in silico D-peptide ligand identification	5
Editorial for special issue on green biomanufacturing	5
Structure-based identification and pathway elucidation of flavonoids in Camptotheca acuminate	5
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	5
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	5
A linear DNA template-based framework for site-specific unnatural amino acid incorporation	4
High-throughput and reliable acquisition of in vivo turnover number fuels precise metabolic engineering	4
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	4
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	4
Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE	4
m4C DNA methylation regulates biosynthesis of daptomycin in Streptomyces roseosporus L30	4
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	4
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	4
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	4
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	4
Engineering a cell-penetrating hyperstable antibody scFv(Ras) – An extraordinary approach to cancer therapeutics	4
An efficient marker recycling system for sequential gene deletion in a deep sea-derived fungus Acremonium sp. HDN16-126	4
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	4
Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function	4
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	4
Unleashing the power of energy storage: Engineering β-oxidation pathways for polyketide production	3
Enhanced cobalamin biosynthesis in Ensifer adhaerens by regulation of key genes with gradient promoters	3
Engineering Saccharomyces cerevisiae for enhanced (–)-α-bisabolol production	3
Heparin mimetics as potential intervention for COVID-19 and their bio-manufacturing	3
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	3
Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system	3
Efficient fatty acid synthesis from methanol in methylotrophic yeast	3
Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli	3
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	3
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	3
Unlocking a new target for streptomycetes strain improvement	3
Structure-guided manipulation of the regioselectivity of the cyclosporine A hydroxylase CYP-sb21 from Sebekia benihana	3

Highly efficient multiplex base editing: One-shot deactivation of eight genes in Shewanella oneidensis MR-1	3
De novo biosynthesis of a vinblastine precursor in Pichia pastoris	3
The ornithine-urea cycle involves fumaric acid biosynthesis in Aureobasidium pullulans var. aubasidani, a green and eco-friendly process for fumaric acid production	3
A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain	3
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	3
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	3
Computational prediction and validation of specific EmbR binding site on PknH	3
Professor Arnold L. (“Arny”) Demain ( 1927-2020): leaving a legacy in the field of Industrial Microbiology and Biotechnology ; a personal appraisal.	3
Engineering microbes to overproduce natural products as agrochemicals	3
Enhancement of phycocyanobilin biosynthesis in Escherichia coli by strengthening the supply of precursor and artificially self-assembly complex	3
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	3
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	3
Unstructured kinetic models to simulate an arabinose switch that decouples cell growth from metabolite production	3
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	3