Synthetic and Systems Biotechnology

Article	Citations
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	70
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	68
Origin and evolution of the main starch biosynthetic enzymes	59
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	55
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	51
Thank you Reviewers list	44
Thank you Reviewers list	43
CO2-elevated cell-free protein synthesis	41
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	38
Gaining access to acetyl-CoA by peroxisomal surface display	37
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	36
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	36
H-NS family protein MvaU downregulates phenazine-1-carboxylic acid (PCA) biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas st	35
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	35
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	32
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	30
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	29
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	29
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	29
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	29
Production of N-acetylglucosamine with Vibrio alginolyticus FA2, an emerging platform for economical unsterile open fermentation	28
The potential of plant proteins as antifungal agents for agricultural applications	27
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	27
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	25
Omics data analysis reveals the system-level constraint on cellular amino acid composition	24

Development of Zn2+-controlled expression system for lactic acid bacteria and its application in engineered probiotics	23
Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production	23
Developing polycistronic expression tool in Yarrowia lipolytica	22
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	22
Engineering artificial cross-species promoters with different transcriptional strengths	22
Discovering peptides and computational investigations of a multiepitope vaccine target Mycobacterium tuberculosis	22
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	21
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	21
Outside Front Cover	20
A generative benchmark for evaluating the performance of fluorescent cell image segmentation	20
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	20
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	20
DNA Storage: The Future Direction for Medical Cold Data Storage	19
Optimized dual-AAV base editor delivery system with enhanced editing efficiency and virion production titer	19
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	19
Functional characterization of triterpene synthases in Cibotium barometz	19
Simultaneous multiplex genome loci editing of Halomonas bluephagenesis using an engineered CRISPR-guided base editor	18
A toolbox for genetic manipulation in intestinal Clostridium symbiosum	17
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	17
Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	16
Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases	16
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	16
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	15
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	15
Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE	15
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	15
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	15
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	15
Advance of tolerance engineering on microbes for industrial production	14
Synthetic biology techniques to tackle heavy metal pollution and poisoning	14
Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae	14
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	14
Strategies to increase tolerance and robustness of industrial microorganisms	14
Single carbon metabolism – A new paradigm for microbial bioprocesses?	14
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	14
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	14
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	13
Engineered microorganisms: A new direction in kidney stone prevention and treatment	13
Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris	13
Degradation strategies of pesticide residue: From chemicals to synthetic biology	13
New N-acylated aminoalkanoic acids from tea roots derived biocontrol agent Clonostachys rosea 15020	12
Editorial for “Special Issue on the 2019 and 2020 iGEM proceedings”	12
Functional investigation of the SAM-dependent methyltransferase RdmB in anthracycline biosynthesis	12
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	12
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	12
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	12
Fructose promotes pyoluteorin biosynthesis via the CbrAB-CrcZ-Hfq/Crc pathway in the biocontrol strain Pseudomonas PA1201	12
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	12
Biosensors for the detection of flaviviruses: A review	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11

Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	11
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	11
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	11
Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi	11
Intelligent tunable CAR-T cell therapy leads the new trend	11
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	11
Combined strategies for improving the heterologous expression of a novel xylanase from Fusarium oxysporum Fo47 in Pichia pastoris	10
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	10
Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses	10
Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette of Vitreoscilla hemoglobin in Bacillus licheniformis	9
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	9
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	9
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	9
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	9
Turning waste gases into valuables	9
Regulation and management of the biosecurity for synthetic biology	9
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	9
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	9
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	8
Efficient fatty acid synthesis from methanol in methylotrophic yeast	8
Enhancing methanol biotransformation for overproducing fatty acid derivatives	8
Synthesis and assembly of full-length cyanophage A-4L genome	8
Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system	8
Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1	8
Design and construction of microbial cell factories based on systems biology	8
Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces	8
Engineered microbes: Making valuable chemicals from waste gases	8
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	8
Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue	7
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	7
Simulating androgen receptor selection in designer yeast	7
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	7
Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli	7
Descriptor-augmented machine learning for enzyme-chemical interaction predictions	7
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	7
Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway of Saccharomyces cerevisiae HJ	7
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	7
A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain	7
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	7
Research progress of pathway and genome evolution in microbes	6
Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae	6
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	6
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	6
Establishment of a selectable marker recycling system for iterative gene editing in Fusarium fujikuroi	6
Integrating chemistry knowledge in large language models via prompt engineering	6
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	6
Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis	6
Transportation engineering for enhanced production of plant natural products in microbial cell factories	6
The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis in Saccharopolyspora spinosa	6
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	6
A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli	6
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	6
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	6
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	6
Study on RNAi-based herbicide for Mikania micrantha	5
SemiSynBio: A new era for neuromorphic computing	5
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	5
Construction of microbial chassis for terpenoid discovery	5
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	5
De novo biosynthesis of a vinblastine precursor in Pichia pastoris	5
The Fold-Illuminator: A low-cost, portable, and disposable incubator-illuminator device	5
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	5
Identification of two new flavone 4'-O-methyltransferases and their application in de novo biosynthesis of (2S)-hesperetin in Yarrowia lipolytica	5
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	5
Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function	5
Customized synthesis of phosphoprotein bearing phosphoserine or its nonhydrolyzable analog	5
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	5
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	4
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	4
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	4
Digitoxose as powerful glycosyls for building multifarious glycoconjugates of natural products and un-natural products	4
Cofactor manipulation to drive biosynthesis of natural products	4
Identification and heterologous expression of the globomycin biosynthetic gene cluster	4
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	4
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	4
Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim.	4
A highly efficient method for genomic deletion across diverse lengths in thermophilic Parageobacillus thermoglucosidasius	4
Identification of a human type XVII collagen fragment with high capacity for maintaining skin health	4

Harnessing the microbial interactions from Apocynum venetum phyllosphere for natural product discovery	4
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	4
Auxotrophy-based curation improves the consensus genome-scale metabolic model of yeast	4
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	4
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	4
Explore the dominant factor in prime editing via a view of DNA processing	4
Enhancement of phycocyanobilin biosynthesis in Escherichia coli by strengthening the supply of precursor and artificially self-assembly complex	4
Methylation in hangtaimycin biosynthesis and its antibacterial activities	4
Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system	4
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	4
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	4
Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides	4
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	4
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	4
Regulatory significance of terminator: A systematic approach for dissecting terminator-mediated enhancement of upstream mRNA stability	4
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol	4
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	4
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	3
A knowledge integration strategy for the selection of a robust multi-stress biomarkers panel for Bacillus subtilis	3
Comparative omics directed gene discovery and rewiring for normal temperature-adaptive red pigment synthesis by polar psychrotrophic fungus Geomyces sp. WNF-15A	3
Riboswitches, from cognition to transformation	3
Heparin mimetics as potential intervention for COVID-19 and their bio-manufacturing	3
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	3
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	3
Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox	3
Biotransformation of ethylene glycol by engineered Escherichia coli	3
Enhancing the activity and succinyl-CoA specificity of 3-ketoacyl-CoA thiolase Tfu_0875 through rational binding pocket engineering	3
Exploiting synthetic regulatory elements for non-dominant microorganisms	3
Microbial soluble aromatic prenyltransferases for engineered biosynthesis	3
Good and bad dispositions between archaea and bacteria in the human gut: New insights from metagenomic survey and co-occurrence analysis	3
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	3
Synthetic polycistronic sequences in eukaryotes	3
Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier	3
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	3
ActinoMation: a literate programming approach for medium-throughput robotic conjugation of Streptomyces spp.	3
Dynamics design of a non-natural transcription factor responding to androst-4-ene-3,17-dione	3
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	3
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	3
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	3
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	3
Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout	3
The ornithine-urea cycle involves fumaric acid biosynthesis in Aureobasidium pullulans var. aubasidani, a green and eco-friendly process for fumaric acid production	3
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	3
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	3
Tissue chips as headway model and incitement technology	3
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	3
Revolutionizing biotechnology advances natural products discovery and industrial processing	3