Synthetic and Systems Biotechnology

Article	Citations
Outside Front Cover	86
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	71
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	60
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	54
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	50
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	45
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	45
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	43
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	43
The functional analysis of NirBD in microorganisms and the efficacies of NirBD on the soil nitrogen storage and N2O emission	43
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	41
Putative membrane protein Cgl2168 and its variant enhance thermotolerance in Corynebacterium glutamicum	40
Degradation strategies of pesticide residue: From chemicals to synthetic biology	36
Thank you Reviewers	35
Genomic and transcriptomic insights into the molecular responses of a biocrust-derived oleaginous microalga Vischeria sp. WL1 to nitrogen depletion and recovery	35
Synergistic engineering PETase reveals loop-region mutations for enhanced catalytic activity and thermal stability	35
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	33
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	33
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	32
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production	31
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	31
Design and construction of microbial cell factories based on systems biology	29
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	29
Transfer learning with pre-trained language models for protein expression level prediction in Escherichia coli	29
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	29

Regulating protein glycosylation modification enhances the synthesis of taxadiene in Saccharomyces cerevisiae	28
Metabolic modulation of TCA cycle by S-nitrosylation in Monascus spp.	28
Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanes deccanensis	28
Engineering riboflavin-overproducing Bacillus subtilis via pathway gene overexpression	27
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	27
Morphology-engineered alleviation of mycelial aggregation in Streptomyces chassis for potentiated production of secondary metabolites	26
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	25
Recombinant production of amaranthin and other betalain variants with yeast cell factories	25
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	25
Cofactor manipulation to drive biosynthesis of natural products	24
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	23
Explore the dominant factor in prime editing via a view of DNA processing	23
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	23
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	23
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	22
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	22
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	21
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	21
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	21
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	21
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	21
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	20
Engineering non-P450 3-hydroxylase for de novo synthesizes catechol-containing compounds in Escherichia coli	20
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	20
Synthetic biotechnology for C1 bio-refinery	20
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	20
A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification	19
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	19
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	19
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	18
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	18
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	18
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	18
From consultors to collaborators – An SOP for advancing ethics engagement in science	17
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	16
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	16
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	16
Way to efficient microbial paclitaxel mass production	16
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	16
Plant metabolism and synthetic biology	16
Malic enzyme-based system for transhydrogenation between nicotinamide cofactors	16
Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects	16
Engineering an ATP-saving mevalonate pathway for high-efficiency S-(+)-linalool production in Serratia marcescens	15
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	15
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	15
Engineered dual-fluorescence functional nucleic acid-based CRISPR/Cas12a biosensor for label-free ratiometric detection of site-specific DNA methylation	15
Engineering Yarrowia lipolytica for nervonic acid production by organelle compartmentalization and dynamic promoter coordination	15
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	15
Biochemical synthesis of taxanes from mevalonate	15
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	14

Engineering Rubisco to enhance CO2 utilization	14
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	14
Metabolic engineering Saccharomyces cerevisiae for de novo biosynthesis of hydroxycinnamoyl glycerols	14
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	14
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	14
Systems metabolic engineering of dual pathways with stage-specific activation for high-level 5-aminolevulinic acid production in Escherichia coli	14
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	14
A systematic discussion and comparison of the construction methods of synthetic microbial community	14
Applications of protein engineering in the microbial synthesis of plant triterpenoids	14
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	14
Topoisomerase II-Like variants enhance acid tolerance and low-pH l-malic acid biosynthesis in metabolic engineered Aspergillus nidulans	14
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	13
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	13
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	13
DNA storage: The future direction for medical cold data storage	13
CO2-elevated cell-free protein synthesis	13
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	13
Synthetic biology techniques to tackle heavy metal pollution and poisoning	12
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	12
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	12
Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in Lactococcus lactis	12
Programmable large-cargo integration: Overcoming size constraints for next-generation gene therapy	12
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	11
Single carbon metabolism – A new paradigm for microbial bioprocesses?	11
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	11
De novo biosynthesis of Asperosaponin VI in Saccharomyces cerevisiae	11
Decoupled tricarboxylic acid cycle and glycolysis of Corynebacterium glutamicum combined with acetate supply promote high-yield biosynthesis of l-homoserine	11
Simulating androgen receptor selection in designer yeast	11
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	11
MTD: A cloud-based omics database and interactive platform for Myceliophthora thermophila	11
Identification, characterization, and application of an NADPH-dependent carbonyl reductase in Saccharomyces cerevisiae AS2.346 for improving production of (13R,17S)-ethyl secol	11
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	11
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	11
Extending dynamic and operational range of the biosensor responding to l-carnitine by directed evolution	11
Engineered microbes: Making valuable chemicals from waste gases	11
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	11
Developing safe and efficient CGBE editor based on Cas-embedding strategy	11
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	11
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	11
Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases	10
Development and application of synthetic biology tools for improved production of human breast milk components	10
Advances in stress-tolerance elements for microbial cell factories	10
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
Engineering ω-transaminase for efficient dihydroxyacetone transamination in serinol biosynthesis starting from methanol	10
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	10
Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library	10
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	10
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
Functions and optimization of soft law in the international governance of synthetic biology: The predicament of hard law vs. the rise of soft law	10
Development of ultrasound-visualized tumor-targeting engineered bacteria for precise tumor therapy	10
Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells	10
Systematic design and evaluation of artificial CO2 assimilation pathways	10
Biotransformation of ethylene glycol by engineered Escherichia coli	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction	9
Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae	9
A smart gene circuit for precise regulation of tumor cell behavior	9
CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae	9
Engineered probiotic-mediated intratumoral delivery and controlled release of bacterial collagenase for cancer therapy	9
Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae	9
Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad in Saccharopolyspora spinosa	9
Rapid detection of genotoxicity employing an engineered high-sensitivity and tight SOS-response biosensor in Escherichia coli MG1655	9
Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via the β-alanine pathway	9
Competitive binding of TeXlnR and TeAmyR regulates cellulase production in Talaromyces endophyticus	9
Enhancing 3-hydroxypropionic acid production from recombinant Saccharomyces cerevisiae for using rice straw hydrolysate and sugar cane industrial waste as substrate	9
Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression	9
Efficient production of astaxanthin in Yarrowia lipolytica through metabolic and enzyme engineering	8
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917	8
Enhanced PrrAB system activation and restricted farnesyl pyrophosphate diversion underlie high coenzyme Q10 accumulation in Rhodobacter sphaeroides HY01	8
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	8
Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology	8
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	8
Corrigendum to “Improving cooperativity of transcription activators by oligomerization domains in mammalian cells” [Synth Syst Biotechnol 8 (1) (2023) 114–120]	8
Multi-gene Co-expression systems in E. coli: From single-vector designs to programmable expression platforms	8
Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in Saccharomyces cerevisiae	8

A new paradigm for the regulation of A40926B0 biosynthesis	8
De novo synthesis of L-2-aminobutyric acid in Escherichia coli based on multi-layered metabolic engineering strategies	8
Engineering Yarrowia lipolytica for high-level β-elemene production via combinatorial metabolic strategies	8
Outside Front Cover	8
Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments	8
Outside Front Cover	8
High-efficiency patatin expression strategies in Komagataella phaffii (Pichia pastoris): Expression cassette toolbox and regulation of protein secretion	8
Thank you Reviewers list	8
Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling	8
Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis	8
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	7
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	7
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	7
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris	7
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	7
System-level characterization of engineered and evolved formatotrophic E. coli strains	7
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	7
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	7
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	7
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	7
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	7
CRISPR-based synthetic biology toolkit development in Candida viswanthii and functional analysis of the stress responsive Ena1-like protein	7
A two-stage metabolome refining pipeline for natural products discovery	7
Efficient fatty acid synthesis from methanol in methylotrophic yeast	7
Enhanced chlorogenic acid production from glucose via systematic metabolic engineering of Saccharomyces cerevisiae	7
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	7
Outside Front Cover	7
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	7
Engineering Yarrowia lipolytica as a yeast cell factory for the de novo production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)	7
Highly biased DNA sequence reconstruction in DNA storage with multi-scale attention mechanism and contrast learning	6
Construction of microbial chassis for terpenoid discovery	6
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	6
Machine learning-assisted rational design and evolution of novel signal peptides in Yarrowia lipolytica	6
Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket	6
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	6
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	6
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	6
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	6
Decoding metabolic trade-offs in Halomonas elongata: Chemostat-based flux remodeling for industrial ectoine biosynthesis	6
High-level production of vitamin K2 in Escherichia coli via modular molecular engineering	6
AmXlnR, a transcription factor involved in xylan degradation and pentose catabolism, enhances pullulan production from xylose in Aureobasidium melanogenum	6
Modulating ion channels with nanobodies	6
iCASRED, a scarless DNA editing tool in E. coli for high-efficiency engineering of natural product biosynthetic gene clusters	6
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	6
Engineering of plant-derived P450scc for de novo biosynthesis of pregnenolone	6
High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast	6
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	6
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	6
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
Thank you Reviewers	6
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	6
Harnessing synthetic biology for tetraterpenoid astaxanthin production: Recent advances and challenges	6
Polyhydroxyalkanoate production during electroactive biofilm formation and stabilization in wetland microbial fuel cells for petroleum hydrocarbon bioconversion	6
Combinatorial engineering of enzyme and pathway for efficient β-farnesene bioproduction in Yarrowia lipolytica	6
T7 RNA polymerase-guided base editor for accelerated continuous evolution in Bacillus subtilis	6
Network of nicotinamide mononucleotide biosynthesis: substrate tracking, rate-limiting enzyme, and regulatory strategy	6
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	6
Truncation of LPD1 promoter and adaptive evolution increase cytosolic acetyl-CoA supply in yeast	5
Design and mathematical analysis of activating transcriptional amplifiers that enable modular temporal control in synthetic juxtacrine circuits	5
Advances in adaptive laboratory evolution applications for Escherichia coli	5
Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities	5
Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilized Saccharomyces cerevisiae cells	5
dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolyspora erythraea	5
Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum	5
Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering	5
Editorial for “Biointelligent manufacturing in Memorium of Arnold (Arny) L. Demain”	5
Discovering the next decade's synthetic biology research trends with ChatGPT	5
Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst	5
Combined metabolic and enzymatic engineering for de novo biosynthesis of δ-tocotrienol in Yarrowia lipolytica	5
Enhancing levan biosynthesis by destroying the strongly acidic environment caused by membrane-bound glucose dehydrogenase (mGDH) in Gluconobacter sp. MP2116	5
Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase	5
Improving cooperativity of transcription activators by oligomerization domains in mammalian cells	5
Mechanisms and biotechnological applications of transcription factors	5
Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR	5
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	5
Metabolic engineering of Escherichia coli for squalene overproduction	5
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	5
Thank you Reviewers	5
Efficient trans-aconitic acid production using systematically metabolic engineered Escherichia coli	5
Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii	5
Engineering the cytochrome P450 to enhance parthenolide production in Saccharomyces cerevisiae	5
Enhanced vitamin B6 production in engineered Escherichia coli via restricted mix-carbon feeding and pressure-controlled fermentation	5
Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin	5
Defect-complementation homologous recombination: A novel strategy for precise genome engineering of virulent phages	4
An efficient CRISPR-Cas12a tool for iterative genome editing, streamlined minimization, and payload engineering of Pseudomonas phage S1	4
The coupling effect of Penicillium baileys W2 in the Aspergillus flavus inhibition and peanut growth promotion	4
Establishment of a selectable marker recycling system for iterative gene editing in Fusarium fujikuroi	4
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	4
Design principles for optogenetic-based targeted protein degradation	4
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	4
Modular reconstruction and multilevel regulation of Saccharomyces cerevisiae to drive the efficient synthesis of asiatic acid	4
Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway of Saccharomyces cerevisiae HJ	4
MicroDFBEST: A dCas12b-derived dual-function base editor with programmable editing characteristics for microbial genetic engineering	4
Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces	4