Synthetic and Systems Biotechnology

Article	Citations
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	79
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	69
Outside Front Cover	60
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	54
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	52
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	49
Metabolic engineering of Glarea lozoyensis for high-level production of pneumocandin B0	47
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	45
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	39
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	39
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	37
Degradation strategies of pesticide residue: From chemicals to synthetic biology	37
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	36
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	32
Thank you Reviewers	32
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	31
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	31
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	31
Research progress of pathway and genome evolution in microbes	30
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	29
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	28
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	27
Cofactor manipulation to drive biosynthesis of natural products	27
Design and construction of microbial cell factories based on systems biology	27
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	27

Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	26
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	26
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	26
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	25
Explore the dominant factor in prime editing via a view of DNA processing	25
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	24
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	24
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	22
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	22
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	22
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	22
Synthetic biotechnology for C1 bio-refinery	21
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	21
Polygalacturonase-inhibiting proteins as an exogenously applied natural solution for prevention of postharvest fungal infections	21
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	21
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	20
Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica	20
Systematic metabolic engineering of Yarrowia lipolytica for efficient production of phytohormone abscisic acid	20
Identification of crucial roles of transcription factor IhfA on high production of free fatty acids in Escherichia coli	20
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	20
Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones	19
CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans	18
Putative functions of EpsK in teichuronic acid synthesis and phosphate starvation in Bacillus licheniformis	17
Engineering and characterization of dehalogenase enzymes from Delftia acidovorans in bioremediation of perfluorinated compounds	17
Protein-protein interface analysis of the non-ribosomal peptide synthetase peptidyl carrier protein and enzymatic domains	17
One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli	17
Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products	17
Improvement of betanin biosynthesis in Saccharomyces cerevisiae by metabolic engineering	17
Way to efficient microbial paclitaxel mass production	17
Plant metabolism and synthetic biology	17
Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins	17
Increasing prediction performance of colorectal cancer disease status using random forests classification based on metagenomic shotgun sequencing data	17
Toehold switch based biosensors for sensing the highly trafficked rosewood Dalbergia maritima	16
Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin	16
Improved l-phenylglycine synthesis by introducing an engineered cofactor self-sufficient system	16
Development of multi-epitope mRNA vaccine against Clostridioides difficile using reverse vaccinology and immunoinformatics approaches	15
A CRISPR/Cas9-based visual toolkit enabling multiplex integration at specific genomic loci in Aspergillus niger	15
Applications of protein engineering in the microbial synthesis of plant triterpenoids	15
Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris	15
Designing a multi-epitope vaccine against Peptostreptococcus anaerobius based on an immunoinformatics approach	15
Aptamer-based cell-free detection system to detect target protein	15
Biochemical synthesis of taxanes from mevalonate	15
CRISPR/Cas12a-based genome editing for cyanophage of Anabeana sp.	15
DNA storage: The future direction for medical cold data storage	14
A systematic discussion and comparison of the construction methods of synthetic microbial community	14
Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	14
Directed evolution of the fusion enzyme for improving astaxanthin biosynthesis in Saccharomyces cerevisiae	14
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	14
Engineering Rubisco to enhance CO2 utilization	14
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	13

Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	13
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	13
CO2-elevated cell-free protein synthesis	13
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	12
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	12
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	11
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	11
Engineered microbes: Making valuable chemicals from waste gases	11
Synthetic biology techniques to tackle heavy metal pollution and poisoning	11
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	11
Single carbon metabolism – A new paradigm for microbial bioprocesses?	11
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	11
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	11
Simulating androgen receptor selection in designer yeast	11
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	11
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	10
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	10
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	10
Developing safe and efficient CGBE editor based on Cas-embedding strategy	10
Exploration and mutagenesis of the germacrene A synthase from Solidago canadensis to enhance germacrene A production in E. coli	10
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	10
CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa	10
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	10
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	10
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	10
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	10
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	10
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	10
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	10
Advances in stress-tolerance elements for microbial cell factories	9
Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux	9
Enhancing abscisic acid production in Botrytis cinerea through metabolic engineering based on a constitutive promoter library	9
Engineering Corynebacterium glutamicum for the efficient production of 3-hydroxypropionic acid from glucose via the β-alanine pathway	9
Decoupled tricarboxylic acid cycle and glycolysis of Corynebacterium glutamicum combined with acetate supply promote high-yield biosynthesis of L-homoserine	9
Deacetoxycephalosporin C synthase (expandase): Research progress and application potential	9
Cyanamide-inducible expression of homing nuclease I−SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae	9
Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad in Saccharopolyspora spinosa	9
Biotransformation of ethylene glycol by engineered Escherichia coli	9
Translocation of subunit PPSE in plipastatin synthase and synthesis of novel lipopeptides	9
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction	9
Rapid detection of genotoxicity employing an engineered high-sensitivity and tight SOS-response biosensor in Escherichia coli MG1655	8
Corrigendum to “Improving cooperativity of transcription activators by oligomerization domains in mammalian cells” [Synth Syst Biotechnol 8 (1) (2023) 114–120]	8
High-efficiency patatin expression strategies in Komagataella phaffii (Pichia pastoris): Expression cassette toolbox and regulation of protein secretion	8
Modular engineering of E. coli coculture for efficient production of resveratrol from glucose and arabinose mixture	8
Filamentous morphology engineering of bacteria by iron metabolism modulation through MagR expression	8
Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae	8
Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling	8
Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis	8
CRISPR/Cas9-based iterative multi-copy integration for improved metabolite yields in Saccharomyces cerevisiae	8
Engineered probiotic-mediated intratumoral delivery and controlled release of bacterial collagenase for cancer therapy	8
Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments	8
Regulatory mechanisms of dopamine metabolism in a marine Meyerozyma guilliermondii GXDK6 under NaCl stress as revealed by integrative multi-omics analysis	8
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials	7
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	7
A two-stage metabolome refining pipeline for natural products discovery	7
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	7
Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production in Saccharomyces cerevisiae	7
Minimizing endogenous cryptic plasmids to construct antibiotic-free expression systems for Escherichia coli Nissle 1917	7
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	7
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	7
Regulatory RNAs in Bacillus subtilis: A review on regulatory mechanism and applications in synthetic biology	7
Outside Front Cover	7
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris	7
System-level characterization of engineered and evolved formatotrophic E. coli strains	7
Thank you Reviewers list	7
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	6
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	6
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	6
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
Systems metabolic engineering of Corynebacterium glutamicum for efficient l-tryptophan production	6
Study on RNAi-based herbicide for Mikania micrantha	6
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	6
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	6
Efficient fatty acid synthesis from methanol in methylotrophic yeast	6
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	6
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	6
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	6
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	6
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	6

Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	6
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	6
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	6
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	6
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	6
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	6
Design and mathematical analysis of activating transcriptional amplifiers that enable modular temporal control in synthetic juxtacrine circuits	5
Design and engineering of logic genetic-enzymatic gates based on the activity of the human CYP2C9 enzyme in permeabilized Saccharomyces cerevisiae cells	5
Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin	5
Biosynthesis of Lacto-N-biose I from starch and N-acetylglucosamine via an in vitro synthetic enzymatic biosystem	5
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	5
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	5
Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst	5
Advances in Adaptive Laboratory Evolution Applications for Escherichia coli	5
Editorial for “Biointelligent manufacturing in Memorium of Arnold (Arny) L. Demain”	5
Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering	5
Polyhydroxyalkanoate production during electroactive biofilm formation and stabilization in wetland microbial fuel cells for petroleum hydrocarbon bioconversion	5
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	5
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	5
Machine Learning-Assisted Rational Design and Evolution of Novel Signal Peptides in Yarrowia lipolytica	5
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	5
Recent progress in engineering Clostridium autoethanogenum to synthesize the biochemicals and biocommodities	5
Expanding catalytic promiscuity of a bifunctional terpene synthase through a single mutation-induced change in hydrogen-bond network within the catalytic pocket	5
Thank you Reviewers	5
Mechanisms and biotechnological applications of transcription factors	5
Discovering the next decade's synthetic biology research trends with ChatGPT	5
Modulating ion channels with nanobodies	5
High-level production of free fatty acids from lignocellulose hydrolysate by co-utilizing glucose and xylose in yeast	5
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	5
Construction of microbial chassis for terpenoid discovery	5
Improving solubility and copy number of taxadiene synthase to enhance the titer of taxadiene in Yarrowia lipolytica	5
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	4
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	4
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	4
The potential of plant proteins as antifungal agents for agricultural applications	4
Engineered microorganisms: A new direction in kidney stone prevention and treatment	4
Efficient production of hydroxysalidroside in Escherichia coli via enhanced glycosylation and semi-rational design of UGT85A1	4
Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production	4
Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integrated Pseudomonas sp. JY-Q with surface-co-displayed PETase and MHETase	4
Improving cooperativity of transcription activators by oligomerization domains in mammalian cells	4
Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid in Corynebacterium glutamicum	4
Origin and evolution of the main starch biosynthetic enzymes	4
Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces	4
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	4
Outside Front Cover	4
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	4
Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against Acinetobacter baumannii	4
H-NS family protein MvaU downregulates phenazine-1-carboxylic acid (PCA) biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas st	4
Bioengineering bacterial encapsulin nanocompartments as targeted drug delivery system	4
Enhancing levan biosynthesis by destroying the strongly acidic environment caused by membrane-bound glucose dehydrogenase (mGDH) in Gluconobacter sp. MP2116	4
Engineering artificial cross-species promoters with different transcriptional strengths	4
Synthesis and assembly of full-length cyanophage A-4L genome	4
Advance of tolerance engineering on microbes for industrial production	4
Biosensors for the detection of flaviviruses: A review	4
Strategies to increase tolerance and robustness of industrial microorganisms	4
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	4
Functional characterization of triterpene synthases in Cibotium barometz	4
finDr: A web server for in silico D-peptide ligand identification	4