Synthetic and Systems Biotechnology

Article	Citations
Fragment antigen binding domains (Fabs) as tools to study assembly-line polyketide synthases	56
Development of Zn2+-controlled expression system for lactic acid bacteria and its application in engineered probiotics	55
Integrative analysis of transcriptome and metabolome reveals the effect of DNA methylation of chalcone isomerase gene in promoter region on Lithocarpus polystachyus Rehd flavonoids	51
Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α	49
Pangenome analysis of Enterobacteria reveals richness of secondary metabolite gene clusters and their associated gene sets	46
Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies	41
Parallel screening and cheminformatics modeling of flavonoid activated aptasensors	36
Two novel aliphatic unsaturated alcohols isolated from a pathogenic fungus Fusarium proliferatum	34
Thank you Reviewers list	34
Thank you Reviewers list	34
O-glycosyltransferases from Homo sapiens contributes to the biosynthesis of Glycyrrhetic Acid 3-O-mono-β-D-glucuronide and Glycyrrhizin in Saccharomyces cerevisiae	33
Synthetic biology techniques to tackle heavy metal pollution and poisoning	32
Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production	30
Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica	30
Origin and evolution of the main starch biosynthetic enzymes	28
The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing	28
Reconstruction and metabolic profiling of the genome-scale metabolic network model of Pseudomonas stutzeri A1501	25
H-NS family protein MvaU downregulates phenazine-1-carboxylic acid (PCA) biosynthesis via binding to an AT-rich region within the promoter of the phz2 gene cluster in the rhizobacterium Pseudomonas st	25
CO2-elevated cell-free protein synthesis	25
Nobachelins, new siderophores from Nocardiopsis baichengensis protecting Caenorhabditis elegans from Pseudomonas aeruginosa infection	23
Advance of tolerance engineering on microbes for industrial production	23
Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae	22
Evaluation and optimization of analytical procedure and sample preparation for polar Streptomyces albus J1074 metabolome profiling	22
Gaining access to acetyl-CoA by peroxisomal surface display	22
Production of N-acetylglucosamine with Vibrio alginolyticus FA2, an emerging platform for economical unsterile open fermentation	21

Conversion of acetate and glyoxylate to fumarate by a cell-free synthetic enzymatic biosystem	20
Identification of a novel multifunctional oxidosqualene cyclase from Zea mays sheds light on the biosynthetic pathway of three pentacyclic triterpenoids	19
A generative benchmark for evaluating the performance of fluorescent cell image segmentation	18
Laccase is a multitasking protein for synthetic gene circuits in the yeast Saccharomyces cerevisiae	18
Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities	18
Outside Front Cover	18
Biomanufacturing of γ-linolenic acid-enriched galactosyldiacylglycerols: Challenges in microalgae and potential in oleaginous yeasts	17
Degradation strategies of pesticide residue: From chemicals to synthetic biology	17
Functional characterization of triterpene synthases in Cibotium barometz	17
Transcriptome-wide analysis of PIP reductase gene family identified a phenylpropene synthase crucial for the biosynthesis of dibenzocyclooctadiene lignans in Schisandra chinensis	16
Sequence and thermodynamic characteristics of terminators revealed by FlowSeq and the discrimination of terminators strength	15
Biotechnological and food synthetic biology potential of platform strain: Bacillus licheniformis	15
A hybrid RNA-protein biosensor for high-throughput screening of adenosylcobalamin biosynthesis	14
Genus-specific secondary metabolome in Allokutzneria and Kibdelosporangium	14
Simultaneous multiplex genome loci editing of Halomonas bluephagenesis using an engineered CRISPR-guided base editor	14
EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications	14
Discovering peptides and computational investigations of a multiepitope vaccine target Mycobacterium tuberculosis	14
Quorum sensing inhibition of hordenine analogs on Pseudomonas aeruginosa and Serratia marcescens	14
A toolbox for genetic manipulation in intestinal Clostridium symbiosum	13
The potential of plant proteins as antifungal agents for agricultural applications	13
Engineering the thermostability of d-lyxose isomerase from Caldanaerobius polysaccharolyticus via multiple computer-aided rational design for efficient synthesis of d-mannose	13
Expression and antimicrobial activity of the recombinant bovine lactoferricin in Pichia pastoris	13
Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products	12
Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton	12
Corrigendum to “Aptamer-based cell-free detection system to detect target protein” [Synth Syst Biotechnol 6 (3) (2021) 209–215]	12
Developing polycistronic expression tool in Yarrowia lipolytica	12
Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry	12
Screening and engineering of high-activity promoter elements through transcriptomics and red fluorescent protein visualization in Rhodobacter sphaeroides	12
Engineering artificial cross-species promoters with different transcriptional strengths	12
Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch	12
Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE	12
Strategies to increase tolerance and robustness of industrial microorganisms	12
Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha	12
Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology	12
Single carbon metabolism – A new paradigm for microbial bioprocesses?	11
Omics data analysis reveals the system-level constraint on cellular amino acid composition	11
Engineered microorganisms: A new direction in kidney stone prevention and treatment	11
Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis	11
Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production	11
Efficient conversion of aromatic and phenylpropanoid alcohols to acids by the cascade biocatalysis of alcohol and aldehyde dehydrogenases	11
Establishment of a selectable marker recycling system for iterative gene editing in Fusarium fujikuroi	10
Editorial for “Special Issue on the 2019 and 2020 iGEM proceedings”	10
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris	10
Turning waste gases into valuables	10
Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold	10
Association between the nasopharyngeal microbiome and metabolome in patients with COVID-19	10
Expression of heat-resistant β-glucosidase in Escherichia coli and its application in the production of gardenia blue	10
Intelligent tunable CAR-T cell therapy leads the new trend	10
Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi	9
Integrating chemistry knowledge in large language models via prompt engineering	9

Engineered microbes: Making valuable chemicals from waste gases	9
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol	9
Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1	9
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in Streptomyces caniferus	9
Functional investigation of the SAM-dependent methyltransferase RdmB in anthracycline biosynthesis	9
Biosensors for the detection of flaviviruses: A review	9
Engineered DNase-inactive Cpf1 variants to improve targeting scope for base editing in E. coli	8
A FAIR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae	8
Enhancing methanol biotransformation for overproducing fatty acid derivatives	8
Nitric oxide synthase-guided genome mining identifies a cytochrome P450 enzyme for olefin nitration in bacterial specialized metabolism	8
Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis	8
Single and multiplexed gene repression in solventogenic Clostridium via Cas12a-based CRISPR interference	8
Altered gut bacterial and metabolic signatures and their interaction in inflammatory bowel disease	8
Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system	8
Construction of an engineered Escherichia coli for effective synthesis of 2′-fucosyllactose via the salvage pathway	8
Efficient production of 2′-fucosyllactose in unconventional yeast Yarrowia lipolytica	8
Regulation and management of the biosecurity for synthetic biology	8
Microbial synthesis of bacitracin: Recent progress, challenges, and prospects	7
Microbial production of cis,cis-muconic acid from aromatic compounds in engineered Pseudomonas	7
Enzymatic properties and inhibition tolerance analysis of key enzymes in β-phenylethanol anabolic pathway of Saccharomyces cerevisiae HJ	7
New dual functional CYP450 gene involves in isoflavone biosynthesis in Glycine max L.	7
Enhanced production of poly-γ-glutamic acid via optimizing the expression cassette of Vitreoscilla hemoglobin in Bacillus licheniformis	7
A rapid nucleic acid detection platform based on phosphorothioate-DNA and sulfur binding domain	7
Analyzing and engineering of the biosynthetic pathway of mollemycin A for enhancing its production	6
Achieving robust synthetic tolerance in industrial E. coli through negative auto-regulation of a DsrA-Hfq module	6
Study on RNAi-based herbicide for Mikania micrantha	6
Research progress of pathway and genome evolution in microbes	6
Cost-effective hybrid long-short read assembly delineates alternative GC-rich Streptomyces hosts for natural product discovery	6
Efficient fatty acid synthesis from methanol in methylotrophic yeast	6
Design-build-test of recombinant Bacillus subtilis chassis cell by lifespan engineering for robust bioprocesses	6
Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters	6
Simulating androgen receptor selection in designer yeast	6
A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli	6
Synthesis and assembly of full-length cyanophage A-4L genome	6
Combined strategies for improving the heterologous expression of a novel xylanase from Fusarium oxysporum Fo47 in Pichia pastoris	6
ECMpy 2.0: A Python package for automated construction and analysis of enzyme-constrained models	6
Design and construction of microbial cell factories based on systems biology	6
Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies	6
Alleviating glucose repression and enhancing respiratory capacity to increase itaconic acid production	6
The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis in Saccharopolyspora spinosa	5
Multi-strategy engineering unusual sugar TDP-l-mycarose biosynthesis to improve the production of 3-O-α-mycarosylerythronolide B in Escherichia coli	5
Fructose promotes pyoluteorin biosynthesis via the CbrAB-CrcZ-Hfq/Crc pathway in the biocontrol strain Pseudomonas PA1201	5
Automated characterization and analysis of expression compatibility between regulatory sequences and metabolic genes in Escherichia coli	5
Transportation engineering for enhanced production of plant natural products in microbial cell factories	5
Engineering Shewanella carassii, a newly isolated exoelectrogen from activated sludge, to enhance methyl orange degradation and bioelectricity harvest	5
End-to-end computational approach to the design of RNA biosensors for detecting miRNA biomarkers of cervical cancer	5
Artificial multi-enzyme cascades and whole-cell transformation for bioconversion of C1 compounds: Advances, challenge and perspectives	5
New N-acylated aminoalkanoic acids from tea roots derived biocontrol agent Clonostachys rosea 15020	5
Novel switchable ECF sigma factor transcription system for improving thaxtomin A production in Streptomyces	5
Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance	4
Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol	4
Harnessing the microbial interactions from Apocynum venetum phyllosphere for natural product discovery	4
Auxotrophy-based curation improves the consensus genome-scale metabolic model of yeast	4
A highly efficient method for genomic deletion across diverse lengths in thermophilic Parageobacillus thermoglucosidasius	4
Thank you Reviewers list	4
Systemically functional characterization of regiospecific flavonoid O-methyltransferases from Glycine max	4
DIProT: A deep learning based interactive toolkit for efficient and effective Protein design	4
Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production	4
Cofactor manipulation to drive biosynthesis of natural products	4
Identification of (-)-bornyl diphosphate synthase from Blumea balsamifera and its application for (-)-borneol biosynthesis in Saccharomyces cerevisiae	4
A CRISPR/Cas9-based single-stranded DNA recombineering system for genome editing of Rhodococcus opacus PD630	4
Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox	4
CgVeA, a light signaling responsive regulator, is involved in regulation of chaetoglobosin A biosynthesis and conidia development in Chaetomium globosum	4
Multi-modular metabolic engineering of heme synthesis in Corynebacterium glutamicum	4
Current state and future perspectives of cytochrome P450 enzymes for C–H and C=C oxygenation	4
A statistical approach to enhance the productivity of Streptomyces baarensis MH-133 for bioactive compounds	4
Exploring the catalytic function and active sites of a novel C-glycosyltransferase from Anemarrhena asphodeloides	4
Genome-wide analysis of UGT gene family identified key gene for the biosynthesis of bioactive flavonol glycosides in Epimedium pubescens Maxim.	4
Assessing the physiological properties of baker's yeast based on single-cell Raman spectrum technology	4
Digitoxose as powerful glycosyls for building multifarious glycoconjugates of natural products and un-natural products	4
Regulatory significance of terminator: A systematic approach for dissecting terminator-mediated enhancement of upstream mRNA stability	4
Engineering caveolin-mediated endocytosis in Saccharomyces cerevisiae	4
A combined strategy for the overproduction of complex ergot alkaloid agroclavine	4
Descriptor-augmented machine learning for enzyme-chemical interaction predictions	4
Acarbose glycosylation by AcbE for the production of acarstatins with enhanced α-amylase inhibitory activity	4
A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris	3
Customized synthesis of phosphoprotein bearing phosphoserine or its nonhydrolyzable analog	3
Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology	3
Characterization of putative mannoprotein in Kluyveromyces lactis for lactase production	3
Enhancement of phycocyanobilin biosynthesis in Escherichia coli by strengthening the supply of precursor and artificially self-assembly complex	3

Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system	3
Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution	3
The ornithine-urea cycle involves fumaric acid biosynthesis in Aureobasidium pullulans var. aubasidani, a green and eco-friendly process for fumaric acid production	3
Explore the dominant factor in prime editing via a view of DNA processing	3
Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase	3
Identification and heterologous expression of the globomycin biosynthetic gene cluster	3
Dynamics design of a non-natural transcription factor responding to androst-4-ene-3,17-dione	3
Synergetic engineering of Escherichia coli for efficient production of l-tyrosine	3
A flexible, modular and versatile functional part assembly toolkit for gene cluster engineering in Streptomyces	3
Construction of microbial chassis for terpenoid discovery	3
SemiSynBio: A new era for neuromorphic computing	3
Synthetic polycistronic sequences in eukaryotes	3
Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function	3
Biotransformation of ethylene glycol by engineered Escherichia coli	3
Enhancing the activity and succinyl-CoA specificity of 3-ketoacyl-CoA thiolase Tfu_0875 through rational binding pocket engineering	3
Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories	3
Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway	3
Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli	3
Methylation in hangtaimycin biosynthesis and its antibacterial activities	3
Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralization	3
Engineering Yarrowia lipolytica to produce nutritional fatty acids: Current status and future perspectives	3
The Fold-Illuminator: A low-cost, portable, and disposable incubator-illuminator device	3
Tissue chips as headway model and incitement technology	2
Engineering Komagataella phaffii to biosynthesize cordycepin from methanol which drives global metabolic alterations at the transcription level	2
Comparison of gut microbiota in autism spectrum disorders and neurotypical boys in China: A case-control study	2
Exploiting synthetic regulatory elements for non-dominant microorganisms	2
Pathway and protein engineering for biosynthesis	2
Coupled strategy based on regulator manipulation and medium optimization empowers the biosynthetic overproduction of lincomycin	2
Genomic scanning enabling discovery of a new antibacterial bicyclic carbamate-containing alkaloid	2
De novo biosynthesis of a vinblastine precursor in Pichia pastoris	2
Advances in the biosynthesis of tetraacetyl phytosphingosine, a key substrate of ceramides	2
Enhancement of polymyxin B1 production by an artificial microbial consortium of Paenibacillus polymyxa and recombinant Corynebacterium glutamicum producing precursor amino acids	2
Identification of a human type XVII collagen fragment with high capacity for maintaining skin health	2
Efficient stereoselective hydroxylation of deoxycholic acid by the robust whole-cell cytochrome P450 CYP107D1 biocatalyst	2
An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization	2
Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects in Saccharomyces cerevisiae	2
Engineering microbes to overproduce natural products as agrochemicals	2
Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01	2
Deletion of COM donor and acceptor domains and the interaction between modules in bacillomycin D produced by Bacillus amyloliquefaciens	2
Microbial soluble aromatic prenyltransferases for engineered biosynthesis	2
WITHDRAWN: Recent Advances in the Microbial Synthesis of Human Hemoglobin	2
Efficient genome editing in Claviceps purpurea using a CRISPR/Cas9 ribonucleoprotein method	2
Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection	2
Structure-based identification and pathway elucidation of flavonoids in Camptotheca acuminate	2
Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier	2