Synthetic Biology

Papers
(The median citation count of Synthetic Biology is 1. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-06-01 to 2026-06-01.)
ArticleCitations
RAPID-DASH: fast and efficient assembly of guide RNA arrays for multiplexed CRISPR-Cas9 applications40
Synthetic biology regulation in Europe: containment, release and beyond30
Construction of a syntrophic Pseudomonas putida consortium with reciprocal substrate processing16
In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade13
Utilizing a cell-free protein synthesis platform for the biosynthesis of a natural product, caffeine12
An artificial protein translation language makes bacteria resistant to viruses12
A mathematical model of cell-free transcription-translation with plasmid crosstalk11
A new Editor-in-chief for Synthetic Biology11
EASY-C: Extraction and Analysis of Small Yeast Chromosomes—A rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids from 10
Construction of a mini-RNA replicon inEscherichia coli10
Minimal shuttle vectors for Saccharomyces cerevisiae9
In - & Out-Cloning : plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly8
GoldenBraid2.0 E. coli : a comprehensive and characterized toolkit for enterics7
Automated cell segmentation for reproducibility in bioimage analysis7
Going beyond size: exploring the metabolic burden in Pseudomonas putida during heterologous protein production7
Fighting fire with fire: engineering a microbe into a therapeutic defense against drug-resistant biofilms6
Writing the dark matter of the human genome into mice to better replicate human disease6
Functional Synthetic Biology5
Harnessing mass spectrometry-based proteomics for continuous directed evolution5
Rewiring cell-free metabolic flux inE. colilysates using a block—push—pull approach5
Special issue: reproducibility in synthetic biology4
Engineering a custom-sized DNA scaffold for more efficient DNA origami-based nucleic acid data storage4
New gene sensors enable precise cell monitoring and control without altering gene sequence4
CryptKeeper: a negative design tool for reducing unintentional gene expression in bacteria3
Cell-free expression of RuBisCO for ATP production in the synthetic cells3
In silico prediction of loop-mediated isothermal amplification using a generalized linear model3
Advancing reproducibility can ease the ‘hard truths’ of synthetic biology3
Successful adaptation of a MinION nanopore for protein sequencing3
Cell-free synthesis of infective phages from in vitro assembled phage genomes for efficient phage engineering and production of large phage libraries2
Biosynthesis of cannabigerol and cannabigerolic acid: the gateways to further cannabinoid production2
BioRxToolbox: a computational framework to streamline genetic circuit design in molecular data communications2
AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots2
Engineering artificial 5′ regulatory sequences for thermostable protein expression in the extremophile Thermus thermophilus2
Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes1
Data hazards in synthetic biology1
Two highly specific growth-coupled biosensor for glycolaldehyde detection across micromolar and millimolar concentrations1
Expanding the Yeast MoClo Toolkit: gene expression control parts for Saccharomyces cerevisiae tested in industrially relevant conditions1
Engineered microbes report levels of freshwater contamination in real time1
Phage-delivered CRISPRi enables bacterial biocomputation1
The naringenin-dependent regulator FdeR can be applied as a NIMPLY gate controlled by naringenin and arabinose1
Variability in genome-engineering source materials: consider your starting point1
Cell-free biosensor with automated acoustic liquid handling for rapid and scalable characterization of cellobiohydrolases on microcrystalline cellulose1
Biosensor characterization: formal methods from the perspective of proteome fractions1
Inert splint-driven oligonucleotide assembly1
Chemically synthesized high-fidelity oligos ≤ 600 nt as building blocks to accelerate complex gene construction in synthetic biology1
Off-target detection of CRISPR-Cas9 nuclease in vitro with CROFT-Seq1
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