OOIR: Observatory of International Research

Papers

(The median citation count of Synthetic Biology is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)

Article	Citations
Synthetic biology regulation in Europe: containment, release and beyond	75
In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade	20
Standardization of inducer-activated broad host range expression modules: debugging and refactoring an alkane-responsive AlkS/PalkB device	19
Development of synthetic biotics as treatment for human diseases	19
Utilizing a cell-free protein synthesis platform for the biosynthesis of a natural product, caffeine	17
An artificial protein translation language makes bacteria resistant to viruses	17
basicsynbio and the BASIC SEVA collection: software and vectors for an established DNA assembly method	16
A new Editor-in-chief for Synthetic Biology	15
An injectable CRISPR therapy instructs B cells to produce anti-HIV antibodies	15
Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform	15
Development of an expression-tunable multiple protein synthesis system in cell-free reactions using T7-promoter-variant series	14
Construction of a mini-RNA replicon inEscherichia coli	13
A toolkit for enhanced reproducibility of RNASeq analysis for synthetic biologists	10
High-yield ‘one-pot’ biosynthesis of raspberry ketone, a high-value fine chemical	8
In- & Out-Cloning: plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly	8
Variability in cell-free expression reactions can impact qualitative genetic circuit characterization	7
Automated cell segmentation for reproducibility in bioimage analysis	7
A computational walk to the hidden peaks of protein performance	7
The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform	7
Writing the dark matter of the human genome into mice to better replicate human disease	6
Functional Synthetic Biology	6
Synthetic directed evolution in plants: unlocking trait engineering and improvement	6
Fighting fire with fire: engineering a microbe into a therapeutic defense against drug-resistant biofilms	6
Evaluation of inducible promoter–riboswitch constructs for heterologous protein expression in the cyanobacterial species Anabaena sp. PCC 7120	6
Methods for measuring the evolutionary stability of engineered genomes to improve their longevity	6

Engineering a custom-sized DNA scaffold for more efficient DNA origami-based nucleic acid data storage	5
Could a simple model of COVID-19 infections be the key to designing better virus-based therapies?	5
New gene sensors enable precise cell monitoring and control without altering gene sequence	5
Rewiring cell-free metabolic flux inE. colilysates using a block—push—pull approach	5
Piece by piece: making plant natural products accessible via heterologous biosynthesis	5
Advancing reproducibility can ease the ‘hard truths’ of synthetic biology	4
Special issue: reproducibility in synthetic biology	4
CryptKeeper: a negative design tool for reducing unintentional gene expression in bacteria	4
Data sharing policies: share well and you shall be rewarded	4
Cofactor-independent RNA editing by a synthetic S-type PPR protein	4
Successful adaptation of a MinION nanopore for protein sequencing	4
Cell-free expression of RuBisCO for ATP production in the synthetic cells	4
Editing Aspergillus terreus using the CRISPR-Cas9 system	4
Enzyme engineering and in vivo testing of a formate reduction pathway	4
In silico prediction of loop-mediated isothermal amplification using a generalized linear model	4
paraSBOLv: a foundation for standard-compliant genetic design visualization tools	3
Rational gRNA design based on transcription factor binding data	3
The transcription activator AtxA from Bacillus anthracis was employed for developing a tight-control, high-level, modulable and stationary-phase-specific transcription activity in Escherichi	3
Screening putative polyester polyurethane degrading enzymes with semi-automated cell-free expression and nitrophenyl probes	3
Biosynthesis of cannabigerol and cannabigerolic acid: the gateways to further cannabinoid production	3
BioRxToolbox: a computational framework to streamline genetic circuit design in molecular data communications	3
Building Biofoundry India: challenges and path forward	3
Signal amplification of araC pBAD using a standardized translation initiation region	3
Biosensor characterization: formal methods from the perspective of proteome fractions	3
Evaluating the persistence and stability of a DNA-barcoded microbial system in a mock home environment	3
AssemblyTron: flexible automation of DNA assembly with Opentrons OT-2 lab robots	3
Cell-free synthesis of infective phages from in vitro assembled phage genomes for efficient phage engineering and production of large phage libraries	3
A universal approach to gene expression engineering	3
The naringenin-dependent regulator FdeR can be applied as a NIMPLY gate controlled by naringenin and arabinose	3