Frontiers in Bioengineering and Biotechnology

Article	Citations
Synthesis of Silver Nanoparticles Mediated by Fungi: A Review	300
Cellulose Biomaterials for Tissue Engineering	238
3D and 4D Printing of Polymers for Tissue Engineering Applications	226
Microbial Proteases Applications	219
Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development	215
A Perspective on Polylactic Acid-Based Polymers Use for Nanoparticles Synthesis and Applications	210
Nanotechnology in Plant Science: To Make a Long Story Short	178
Use of Nanoparticles in Tissue Engineering and Regenerative Medicine	169
Enzymatic Modifications of Chitin, Chitosan, and Chitooligosaccharides	162
Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing	162
Engineering Organoid Vascularization	160
Challenges With the Development of Biomaterials for Sustainable Tissue Engineering	151
The Use of Microalgae for Coupling Wastewater Treatment With CO2 Biofixation	141
Modern Trends for Peripheral Nerve Repair and Regeneration: Beyond the Hollow Nerve Guidance Conduit	132
New Applications of Synthetic Biology Tools for Cyanobacterial Metabolic Engineering	129
Titanium–Tissue Interface Reaction and Its Control With Surface Treatment	126
Mesenchymal Stem Cell Therapy for Osteoarthritis: The Critical Role of the Cell Secretome	122
Methods for Segmentation and Classification of Digital Microscopy Tissue Images	117
Bacterial Valorization of Lignin: Strains, Enzymes, Conversion Pathways, Biosensors, and Perspectives	98
Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction	97
Nano-Enhanced Drug Delivery and Therapeutic Ultrasound for Cancer Treatment and Beyond	97
Determination of Corneal Biomechanical Behavior in-vivo for Healthy Eyes Using CorVis ST Tonometry: Stress-Strain Index	97
Multiple and Promising Applications of Strontium (Sr)-Containing Bioactive Glasses in Bone Tissue Engineering	96
Advances and Current Challenges in Intestinal in vitro Model Engineering: A Digest	96
Development of L-Asparaginase Biobetters: Current Research Status and Review of the Desirable Quality Profiles	96

Plants Developed by New Genetic Modification Techniques—Comparison of Existing Regulatory Frameworks in the EU and Non-EU Countries	91
A Robust Longitudinal Co-culture of Obligate Anaerobic Gut Microbiome With Human Intestinal Epithelium in an Anoxic-Oxic Interface-on-a-Chip	88
Biomethane Production From Lignocellulose: Biomass Recalcitrance and Its Impacts on Anaerobic Digestion	86
Hypoxia Conditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Induce Increased Vascular Tube Formation in vitro	86
NanoBRET: The Bright Future of Proximity-Based Assays	86
A Random Forest Sub-Golgi Protein Classifier Optimized via Dipeptide and Amino Acid Composition Features	85
Recent Advances in Nanomaterials-Based Chemo-Photothermal Combination Therapy for Improving Cancer Treatment	83
Collagen-Based Tissue Engineering Strategies for Vascular Medicine	82
A Review of Structural and Biomechanical Changes in the Cornea in Aging, Disease, and Photochemical Crosslinking	81
Corneal Repair and Regeneration: Current Concepts and Future Directions	79
Photodynamic Therapy Based on Graphene and MXene in Cancer Theranostics	78
Gene-Expressing Liposomes as Synthetic Cells for Molecular Communication Studies	76
Cell Cytometry: Review and Perspective on Biotechnological Advances	76
Encapsulation and Controlled Release of Resveratrol Within Functionalized Mesoporous Silica Nanoparticles for Prostate Cancer Therapy	75
Peptide-Based Functional Biomaterials for Soft-Tissue Repair	74
Applications of Iron Oxide-Based Magnetic Nanoparticles in the Diagnosis and Treatment of Bacterial Infections	74
Bioproducts From Euglena gracilis: Synthesis and Applications	71
3D-Printed PCL/PPy Conductive Scaffolds as Three-Dimensional Porous Nerve Guide Conduits (NGCs) for Peripheral Nerve Injury Repair	71
Mesenchymal Stromal Cell-Based Bone Regeneration Therapies: From Cell Transplantation and Tissue Engineering to Therapeutic Secretomes and Extracellular Vesicles	68
Overcoming Physiological Barriers to Nanoparticle Delivery—Are We There Yet?	65
Genetically Engineered Crops: Importance of Diversified Integrated Pest Management for Agricultural Sustainability	65
Future Trends in Synthetic Biology—A Report	64
Exploring the Potential of Cell-Free Protein Synthesis for Extending the Abilities of Biological Systems	64
Macrophage-Driven Biomaterial Degradation Depends on Scaffold Microarchitecture	63
Cell Encapsulation Within Alginate Microcapsules: Immunological Challenges and Outlook	62
Osteochondral Tissue Chip Derived From iPSCs: Modeling OA Pathologies and Testing Drugs	61
Non-invasive Production of Multi-Compartmental Biodegradable Polymer Microneedles for Controlled Intradermal Drug Release of Labile Molecules	59
Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing	59
Integrative Bioinformatics Approaches to Map Potential Novel Genes and Pathways Involved in Ovarian Cancer	58
Impaired Wound Healing of Alveolar Lung Epithelial Cells in a Breathing Lung-On-A-Chip	57
Exploring Instructive Physiological Signaling with the Bioelectric Tissue Simulation Engine	57
Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication	56
Bone Marrow Mesenchymal Stem Cells' Secretome Exerts Neuroprotective Effects in a Parkinson's Disease Rat Model	56
Enzymatic Bioremediation of Organophosphate Compounds—Progress and Remaining Challenges	55
Nanomedicine Approaches for the Pulmonary Treatment of Cystic Fibrosis	54
Normalization Methods for the Analysis of Unbalanced Transcriptome Data: A Review	54
A Clostridium Group IV Species Dominates and Suppresses a Mixed Culture Fermentation by Tolerance to Medium Chain Fatty Acids Products	54
AOPs-SVM: A Sequence-Based Classifier of Antioxidant Proteins Using a Support Vector Machine	53
Cytotoxicity-Related Bioeffects Induced by Nanoparticles: The Role of Surface Chemistry	52
Hazard Assessment of Polymeric Nanobiomaterials for Drug Delivery: What Can We Learn From Literature So Far	52
Sequential Co-immobilization of Enzymes in Metal-Organic Frameworks for Efficient Biocatalytic Conversion of Adsorbed CO2 to Formate	52
Biosensors for On-Farm Diagnosis of Mastitis	51
Development and Validation of a Deep Learning-Based Model Using Computed Tomography Imaging for Predicting Disease Severity of Coronavirus Disease 2019	49
Three Dimensional Printing Bilayer Membrane Scaffold Promotes Wound Healing	49
Dysregulation of Signaling Pathways Due to Differentially Expressed Genes From the B-Cell Transcriptomes of Systemic Lupus Erythematosus Patients – A Bioinformatics Approach	49
An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)	49
Metabolic Engineering and Synthetic Biology: Synergies, Future, and Challenges	48
Classifying Promoters by Interpreting the Hidden Information of DNA Sequences via Deep Learning and Combination of Continuous FastText N-Grams	48
Raman Spectroscopy: Guiding Light for the Extracellular Matrix	48
Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering	48

Perspective on Pulsed Electric Field Treatment in the Bio-based Industry	48
Improving Reproducibility in Synthetic Biology	47
A Microfluidic System for Studying the Effects of Disturbed Flow on Endothelial Cells	47
Polymeric Approaches to Reduce Tissue Responses Against Devices Applied for Islet-Cell Encapsulation	47
Cytocompatible and Anti-bacterial Adhesion Nanotextured Titanium Oxide Layer on Titanium Surfaces for Dental and Orthopedic Implants	47
Investigation of the Antibacterial Activity and in vivo Cytotoxicity of Biogenic Silver Nanoparticles as Potent Therapeutics	46
Nanotheranostics Targeting the Tumor Microenvironment	46
Functionalising Collagen-Based Scaffolds With Platelet-Rich Plasma for Enhanced Skin Wound Healing Potential	44
Biological Parts for Kluyveromyces marxianus Synthetic Biology	44
A Direct Comparison of Biplanar Videoradiography and Optical Motion Capture for Foot and Ankle Kinematics	43
Enzymatic Degradation of Biofilm by Metalloprotease From Microbacterium sp. SKS10	43
Perspectives on Harmful Algal Blooms (HABs) and the Cyberbiosecurity of Freshwater Systems	43
Addressing Patient Specificity in the Engineering of Tumor Models	43
Molecular Modeling for Nanomaterial–Biology Interactions: Opportunities, Challenges, and Perspectives	43
Systematic Characterization of Circular RNA-Associated CeRNA Network Identified Novel circRNA Biomarkers in Alzheimer's Disease	42
Maximizing the Efficiency of Vanillin Production by Biocatalyst Enhancement and Process Optimization	42
Deep Learning With Sampling in Colon Cancer Histology	42
Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities	42
Engineered Cell-Based Therapeutics: Synthetic Biology Meets Immunology	41
Using Bioactive Glasses in the Management of Burns	41
Inflammation and Bone Repair: From Particle Disease to Tissue Regeneration	40
Occurrence of Soil Fungi in Antarctic Pristine Environments	40
Composite Hydrogels in Three-Dimensional in vitro Models	40
Bioactivation Routes of Gelatin-Based Scaffolds to Enhance at Nanoscale Level Bone Tissue Regeneration	40
Biomechanical Investigation of Disturbed Hemodynamics-Induced Tissue Degeneration in Abdominal Aortic Aneurysms Using Computational and Experimental Techniques	40
Drug Delivery From Polymer-Based Nanopharmaceuticals—An Experimental Study Complemented by Simulations of Selected Diffusion Processes	39
Optically Active Nanomaterials for Bioimaging and Targeted Therapy	39
Identification of Prognostic and Metastatic Alternative Splicing Signatures in Kidney Renal Clear Cell Carcinoma	38
3D Microfluidic Bone Tumor Microenvironment Comprised of Hydroxyapatite/Fibrin Composite	38
Statistical Shape Modeling of Skeletal Anatomy for Sex Discrimination: Their Training Size, Sexual Dimorphism, and Asymmetry	38
Biological Response to Carbon-Family Nanomaterials: Interactions at the Nano-Bio Interface	38
Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle	38
Magnetic Separation in Bioprocessing Beyond the Analytical Scale: From Biotechnology to the Food Industry	38
Surface Modification of Aliphatic Polyester to Enhance Biocompatibility	38
Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production	37
Staining Invariant Features for Improving Generalization of Deep Convolutional Neural Networks in Computational Pathology	37
Preparation of Antimicrobial Hyaluronic Acid/Quaternized Chitosan Hydrogels for the Promotion of Seawater-Immersion Wound Healing	37
Production of Melanins With Recombinant Microorganisms	37
Microalgal Protein Extraction From Chlorella vulgaris FSP-E Using Triphasic Partitioning Technique With Sonication	37
Neural Lineage Differentiation From Pluripotent Stem Cells to Mimic Human Brain Tissues	37
Advantages of Hydrogel-Based 3D-Printed Enzyme Reactors and Their Limitations for Biocatalysis	36
Vascular Niche in Lung Alveolar Development, Homeostasis, and Regeneration	36
Long-Term Behavior of Defined Mixed Cultures of Geobacter sulfurreducens and Shewanella oneidensis in Bioelectrochemical Systems	36
Laminin-Coated Electrospun Regenerated Silk Fibroin Mats Promote Neural Progenitor Cell Proliferation, Differentiation, and Survival in vitro	36
Factors Influencing Recombinant Protein Secretion Efficiency in Gram-Positive Bacteria: Signal Peptide and Beyond	35
Mechanisms of Adverse Local Tissue Reactions to Hip Implants	35
A Systematic Review and Meta-Analysis of Clinical Effectiveness and Safety of Hydrogel Dressings in the Management of Skin Wounds	35
The Extracellular Matrix and Biocompatible Materials in Glioblastoma Treatment	35
Beyond the Present Constraints That Prevent a Wide Spread of Tissue Engineering and Regenerative Medicine Approaches	35
Regulatory Status of Genome-Edited Organisms Under the Japanese Cartagena Act	35
Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model	34
Fibronectin Functionalized Electrospun Fibers by Using Benign Solvents: Best Way to Achieve Effective Functionalization	33
Biomechanical Impact of the Sclera on Corneal Deformation Response to an Air-Puff: A Finite-Element Study	33
Technology Advancements in Blood Coagulation Measurements for Point-of-Care Diagnostic Testing	33
Three-Layered Silk Fibroin Tubular Scaffold for the Repair and Regeneration of Small Caliber Blood Vessels: From Design to in vivo Pilot Tests	33
Multi-Factorial-Guided Media Optimization for Enhanced Biomass and Lipid Formation by the Oleaginous Yeast Cutaneotrichosporon oleaginosus	33
Specifications for Innovative, Enabling Biomaterials Based on the Principles of Biocompatibility Mechanisms	33
Synovium-Derived Mesenchymal Stem Cell Transplantation in Cartilage Regeneration: A PRISMA Review of in vivo Studies	33