ACS Synthetic Biology

Article	Citations
Transcription-Factor-based Biosensor Engineering for Applications in Synthetic Biology	84
Statistical Design of Experiments for Synthetic Biology	68
Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput	64
Engineering Wired Life: Synthetic Biology for Electroactive Bacteria	61
Enabling Biological Nitrogen Fixation for Cereal Crops in Fertilized Fields	56
Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2	55
Systematic Quantification of Sequence and Structural Determinants Controlling mRNA stability in Bacterial Operons	54
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design	50
Cell-Free Gene Expression Dynamics in Synthetic Cell Populations	50
Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production	50
A Whole-Cell Biosensor for Point-of-Care Detection of Waterborne Bacterial Pathogens	49
3D Bioprinting in Skin Related Research: Recent Achievements and Application Perspectives	48
Rational Promoter Engineering Enables Robust Terpene Production in Microalgae	47
Rewiring Central Carbon Metabolism Ensures Increased Provision of Acetyl-CoA and NADPH Required for 3-OH-Propionic Acid Production	47
Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles	47
Optogenetic Amplification Circuits for Light-Induced Metabolic Control	47
A User’s Guide to Golden Gate Cloning Methods and Standards	44
CRISPR-Cas13 System as a Promising and Versatile Tool for Cancer Diagnosis, Therapy, and Research	44
Simultaneous Improvement of Limonene Production and Tolerance in Yarrowia lipolytica through Tolerance Engineering and Evolutionary Engineering	43
Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9	43
Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris	42
Modular Synthetic Biology Toolkit for Filamentous Fungi	41
Improving the Heterologous Production of Fungal Peroxygenases through an Episomal Pichia pastoris Promoter and Signal Peptide Shuffling System	41
Synthetic Virus-like Particles for Glutathione Biosynthesis	41
Kinetics of RNA and RNA:DNA Hybrid Strand Displacement	40

Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917	40
A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics	39
Encapsulin Nanocontainers as Versatile Scaffolds for the Development of Artificial Metabolons	39
Efficient Biosynthesis of (2S)-Eriodictyol from (2S)-Naringenin in Saccharomyces cerevisiae through a Combination of Promoter Adjustment and Directed Evolution	38
CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides Species	38
Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation	37
In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii	37
Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments	37
RPA/CRISPR/Cas12a-Based On-Site and Rapid Nucleic Acid Detection of Toxoplasma gondii in the Environment	37
Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network	37
A Golden-Gate Based Cloning Toolkit to Build Violacein Pathway Libraries in Yarrowia lipolytica	36
Design and Characterization of Rapid Optogenetic Circuits for Dynamic Control in Yeast Metabolic Engineering	36
Evolving a Generalist Biosensor for Bicyclic Monoterpenes	36
Systematically Engineered Fatty Acid Catabolite Pathway for the Production of (2S)-Naringenin in Saccharomyces cerevisiae	36
High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae	36
Optogenetic Control of Microbial Consortia Populations for Chemical Production	35
Engineered Microbial Routes for Human Milk Oligosaccharides Synthesis	35
Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production	35
Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems	35
Prime Editing Guide RNA Design Automation Using PINE-CONE	34
Precise Prediction of Promoter Strength Based on a De Novo Synthetic Promoter Library Coupled with Machine Learning	34
Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide	33
Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13	33
A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines	33
Metabolic Engineering of Escherichia coli for De Novo Production of 1,5-Pentanediol from Glucose	32
Recognition of DNA Target Formulations by CRISPR-Cas12a Using a dsDNA Reporter	32
Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment	32
Metabolic Engineering of Gas-Fermenting Clostridium ljungdahlii for Efficient Co-production of Isopropanol, 3-Hydroxybutyrate, and Ethanol	31
Label-Free Detection of Transgenic Crops Using an Isothermal Amplification Reporting CRISPR/Cas12 Assay	31
A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology	31
The Marburg Collection: A Golden Gate DNA Assembly Framework for Synthetic Biology Applications in Vibrio natriegens	30
evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library	30
A Protein-Engineered, Enhanced Yeast Display Platform for Rapid Evolution of Challenging Targets	30
Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast	30
Standardization of Synthetic Biology Tools and Assembly Methods for Saccharomyces cerevisiae and Emerging Yeast Species	29
CRISPR-Based Diagnostics: Challenges and Potential Solutions toward Point-of-Care Applications	29
Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei	29
High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas	29
Constructing Cell-Free Expression Systems for Low-Cost Access	28
Programmable Fusion and Differentiation of Synthetic Minimal Cells	28
Engineering the Lipid and Fatty Acid Metabolism in Yarrowia lipolytica for Sustainable Production of High Oleic Oils	28
The Bacillus subtilis Minimal Genome Compendium	27
Characterization of a Group of UDP-Glycosyltransferases Involved in the Biosynthesis of Triterpenoid Saponins of Panax notoginseng	27
Continuous Cell-Free Replication and Evolution of Artificial Genomic DNA in a Compartmentalized Gene Expression System	27
Improve the Biosynthesis of Baicalein and Scutellarein via Manufacturing Self-Assembly Enzyme Reactor In Vivo	27
Engineering Yeast Yarrowia lipolytica for Methanol Assimilation	27
Biotechnological Production of 2′-Fucosyllactose: A Prevalent Fucosylated Human Milk Oligosaccharide	27
A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae	27
Efficient Production of 2′-Fucosyllactose from l-Fucose via Self-Assembling Multienzyme Complexes in Engineered Escherichia coli	27
YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants	27

Membrane Augmented Cell-Free Systems: A New Frontier in Biotechnology	26
Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus	26
Establishment of a Biosensor-based High-Throughput Screening Platform for Tryptophan Overproduction	26
Evolution of Functionally Enhanced α-l-Threofuranosyl Nucleic Acid Aptamers	26
Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom	25
Low-Bias Manipulation of DNA Oligo Pool for Robust Data Storage	25
Rerouting Fluxes of the Central Carbon Metabolism and Relieving Mechanism-Based Inactivation of l-Aspartate-α-decarboxylase for Fermentative Production of β-Alanine in Escherichia coli	25
Construction of an l-Tyrosine Chassis in Pichia pastoris Enhances Aromatic Secondary Metabolite Production from Glycerol	25
Engineering Strategies to Overcome the Stability–Function Trade-Off in Proteins	24
Biosynthesis of a Therapeutically Important Nicotinamide Mononucleotide through a Phosphoribosyl Pyrophosphate Synthetase 1 and 2 Engineered Strain of Escherichia coli	24
Evolving Small-Molecule Biosensors with Improved Performance and Reprogrammed Ligand Preference Using OrthoRep	24
DIVERSIFY: A Fungal Multispecies Gene Expression Platform	24
Increased Lipid Production in Yarrowia lipolytica from Acetate through Metabolic Engineering and Cosubstrate Fermentation	24
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries	24
Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host	24
Systems Metabolic Engineering of Escherichia coli Coculture for De Novo Production of Genistein	23
Optimization of a p-Coumaric Acid Biosensor System for Versatile Dynamic Performance	23
Curli-Mediated Self-Assembly of a Fibrous Protein Scaffold for Hydroxyapatite Mineralization	23
Engineering the Reductive TCA Pathway to Dynamically Regulate the Biosynthesis of Adipic Acid in Escherichia coli	23
Novel Prokaryotic CRISPR-Cas12a-Based Tool for Programmable Transcriptional Activation and Repression	23
Charge Engineering Improves the Performance of Bst DNA Polymerase Fusions	22
Cheetah: A Computational Toolkit for Cybergenetic Control	22
Glycosylation Modification Enhances (2S)-Naringenin Production in Saccharomyces cerevisiae	22
Immortalized Bovine Satellite Cells for Cultured Meat Applications	22
Highly Reversible Tunable Thermal-Repressible Split-T7 RNA Polymerases (Thermal-T7RNAPs) for Dynamic Gene Regulation	22
Sequence Preference and Initiator Promiscuity for De Novo DNA Synthesis by Terminal Deoxynucleotidyl Transferase	22
Construction and Characterization of a Gradient Strength Promoter Library for Fine-Tuned Gene Expression in Bacillus licheniformis	22
Combining CRISPR–Cpf1 and Recombineering Facilitates Fast and Efficient Genome Editing in Escherichia coli	22
Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms	22
High-Yielding Terpene-Based Biofuel Production in Rhodobacter capsulatus	22
Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters	21
Cell Catalysis of Citrate to Itaconate by Engineered Halomonas bluephagenesis	21
Automated Rational Strain Construction Based on High-Throughput Conjugation	21
A Novel and Efficient Genome Editing Tool Assisted by CRISPR-Cas12a/Cpf1 for Pichia pastoris	21
Emergent Biological Endurance Depends on Extracellular Matrix Composition of Three-Dimensionally Printed Escherichia coli Biofilms	21
Construction of an rAAV Producer Cell Line through Synthetic Biology	20
Engineering Antisense Oligonucleotides as Antibacterial Agents That Target FMN Riboswitches and Inhibit the Growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia co	20
Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis	20
Actinosomes: Condensate-Templated Containers for Engineering Synthetic Cells	20
Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli	20
Resveratrol Production from Hydrothermally Pretreated Eucalyptus Wood Using Recombinant Industrial Saccharomyces cerevisiae Strains	20
Saccharomyces cerevisiae Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins	20
Multilayer Genetic Circuits for Dynamic Regulation of Metabolic Pathways	20
Repurposing the Endogenous Type I-E CRISPR/Cas System for Gene Repression in Gluconobacter oxydans WSH-003	20
Bioengineering Self-Organizing Signaling Centers to Control Embryoid Body Pattern Elaboration	20
Application of Cell-Free Protein Synthesis System for the Biosynthesis of l-Theanine	19
De Novo Production of Plant 4′-Deoxyflavones Baicalein and Oroxylin A from Ethanol in Crabtree-Negative Yeast	19
Synergistic Metabolism of Glucose and Formate Increases the Yield of Short-Chain Organic Acids in Escherichia coli	19
An Aldolase-Based New Pathway for Bioconversion of Formaldehyde and Ethanol into 1,3-Propanediol in Escherichia coli	19
Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules	19
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts	19
Metabolic Engineering of Aspartic Acid Supply Modules for Enhanced Production of Bacitracin in Bacillus licheniformis	19
Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity	19
A Toolkit for Precise, Multigene Control in Saccharomyces cerevisiae	19
Optogenetic Control of Bacterial Expression by Red Light	19
Heterologous Expression of Mersacidin in Escherichia coli Elucidates the Mode of Leader Processing	19
Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites	19
Efficient Production of 1,3-Propanediol from Diverse Carbohydrates via a Non-natural Pathway Using 3-Hydroxypropionic Acid as an Intermediate	19
Metabolic Engineering of Escherichia coli for High-Yield Production of (R)-1,3-Butanediol	19
Agent-Based Modeling of Microbial Communities	19
Fine-Tuning Multi-Gene Clusters via Well-Characterized Gene Expression Regulatory Elements: Case Study of the Arginine Synthesis Pathway in C. glutamicum	19
Bioremediation of Industrial Pollutants by Insects Expressing a Fungal Laccase	19
Synthetic Biology in Chimeric Antigen Receptor T (CAR T) Cell Engineering	19
Functional Comparison of Laboratory-Evolved XNA Polymerases for Synthetic Biology	19
Combining Metabolic and Monoterpene Synthase Engineering for de Novo Production of Monoterpene Alcohols in Escherichia coli	19
Engineering T4 Bacteriophage for In Vivo Display by Type V CRISPR-Cas Genome Editing	18
Control of Multigene Expression Stoichiometry in Mammalian Cells Using Synthetic Promoters	18
Development of a Transcription Factor-Based Diamine Biosensor in Corynebacterium glutamicum	18
Signal Peptide Efficiency: From High-Throughput Data to Prediction and Explanation	18
A Biosensor for Detection of Indole Metabolites	18
Generation of Synthetic Shuttle Vectors Enabling Modular Genetic Engineering of Cyanobacteria	18
Receptor Elimination by E3 Ubiquitin Ligase Recruitment (REULR): A Targeted Protein Degradation Toolbox	18
Highly Efficient Production of Menaquinone-7 from Glucose by Metabolically Engineered Escherichia coli	18
Secretory Production of Tocotrienols in Saccharomyces cerevisiae	18
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	18
Engineering the Biosynthesis of Late-Stage Vinblastine Precursors Precondylocarpine Acetate, Catharanthine, Tabersonine in Nicotiana benthamiana	18
High-Throughput Aminoacyl-tRNA Synthetase Engineering for Genetic Code Expansion in Yeast	18
Nisin- and Ripcin-Derived Hybrid Lanthipeptides Display Selective Antimicrobial Activity against Staphylococcus aureus	18
Genome Engineering of the Fast-Growing Mycoplasma feriruminatoris toward a Live Vaccine Chassis	18
Targeting glmS Ribozyme with Chimeric Antisense Oligonucleotides for Antibacterial Drug Development	18

Trade-Offs in Biosensor Optimization for Dynamic Pathway Engineering	18
Optimization of Heavy Metal Sensors Based on Transcription Factors and Cell-Free Expression Systems	18
Improved Production of Fengycin in Bacillus subtilis by Integrated Strain Engineering Strategy	18
Light-Induced Patterning of Electroactive Bacterial Biofilms	18
Quantitative Yeast–Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein–Protein Interactions	18
Rational Design of the N-Terminal Coding Sequence for Regulating Enzyme Expression in Bacillus subtilis	18
Decoupling Protein Production from Cell Growth Enhances the Site-Specific Incorporation of Noncanonical Amino Acids in E. coli	18
Recombinant Protein Stability in Cyanobacteria	18
A Sensitive and Specific Fluorescent RT-LAMP Assay for SARS-CoV-2 Detection in Clinical Samples	18
Improving the Level of the Tyrosine Biosynthesis Pathway in Saccharomyces cerevisiae through HTZ1 Knockout and Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis	18
A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers	18
A Whole-Cell Bacterial Biosensor for Blood Markers Detection in Urine	18
Development of a Highly Efficient Copper-Inducible GAL Regulation System (CuIGR) in Saccharomyces cerevisiae	18
Engineering Yarrowia lipolytica to Produce Tailored Chain-Length Fatty Acids and Their Derivatives	17
Engineering of a Biosensor in Response to Malate in Bacillus licheniformis	17
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast	17
Synthetic Lateral Inhibition in Periodic Pattern Forming Microbial Colonies	17
Tuning Gene Expression by Phosphate in the Methanogenic Archaeon Methanococcus maripaludis	17
High-Titer Production of Olivetolic Acid and Analogs in Engineered Fungal Host Using a Nonplant Biosynthetic Pathway	17
Efficient Synthesis of Phycocyanobilin by Combinatorial Metabolic Engineering in Escherichia coli	17
Actomyosin-Driven Division of a Synthetic Cell	17
Precise Thermal Regulation of Engineered Bacteria Secretion for Breast Cancer Treatment In Vivo	17
Exploration of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase Activity in Yeast	17
Rational Design of a Split Flavin-Based Fluorescent Reporter	17
Engineering Gac/Rsm Signaling Cascade for Optogenetic Induction of the Pathogenicity Switch in Pseudomonas aeruginosa	17
Engineering a Central Carbon Metabolism Pathway to Increase the Intracellular Acetyl-CoA Pool in Synechocystis sp. PCC 6803 Grown under Photomixotrophic Conditions	17
Optogenetics in Sinorhizobium meliloti Enables Spatial Control of Exopolysaccharide Production and Biofilm Structure	17
Engineering Pheromone-Mediated Quorum Sensing with Enhanced Response Output Increases Fucosyllactose Production in Saccharomyces cerevisiae	17
Metabolic Engineering of Yarrowia lipolytica for Terpenoid Production: Tools and Strategies	17
Reprogramming Synthetic Cells for Targeted Cancer Therapy	17
A Cascade Signal Amplification Based on Dynamic DNA Nanodevices and CRISPR/Cas12a Trans-cleavage for Highly Sensitive MicroRNA Sensing	17
Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast	16
Antisense RNA Interference-Enhanced CRISPR/Cas9 Base Editing Method for Improving Base Editing Efficiency in Streptomyces lividans 66	16
Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts	16
GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants	16
Regulatory Part Engineering for High-Yield Protein Synthesis in an All-Streptomyces-Based Cell-Free Expression System	16
Genetic Diversity for Accelerating Microbial Adaptive Laboratory Evolution	16
Toward Full-Stack In Silico Synthetic Biology: Integrating Model Specification, Simulation, Verification, and Biological Compilation	16
Multidimensional Characterization of Parts Enhances Modeling Accuracy in Genetic Circuits	16
Characterization of Constitutive Promoters for the Elicitation of Secondary Metabolites in Myxobacteria	16
Improved Bioproduction of 1-Octanol Using Engineered Synechocystis sp. PCC 6803	16
Yeast Cell Surface Engineering of a Nicotinamide Riboside Kinase for the Production of β-Nicotinamide Mononucleotide via Whole-Cell Catalysis	16
Promiscuity Characteristics of Versatile Plant Glycosyltransferases for Natural Product Glycodiversification	16
Efficient Production of a Functional Human Milk Oligosaccharide 3′-Sialyllactose in Genetically Engineered Escherichia coli	16
Functional Expression and Characterization of the Highly Promiscuous Lanthipeptide Synthetase SyncM, Enabling the Production of Lanthipeptides with a Broad Range of Ring Topologies	16
Direct Comparison of d-DNA and l-DNA Strand-Displacement Reactions in Living Mammalian Cells	16
A Computational Framework for Identifying Promoter Sequences in Nonmodel Organisms Using RNA-seq Data Sets	16
Komagataeibacter Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria	16
Engineered Protein Nanocages for Concurrent RNA and Protein Packaging In Vivo	16
Generative Artificial Intelligence GPT-4 Accelerates Knowledge Mining and Machine Learning for Synthetic Biology	16
Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits	16
Escherichia coli Promoters with Consistent Expression throughout the Murine Gut	16
Living Therapeutics: The Next Frontier of Precision Medicine	16
CRISPR-Based Construction of a BL21 (DE3)-Derived Variant Strain Library to Rapidly Improve Recombinant Protein Production	15
An Aspergillus nidulans Platform for the Complete Cluster Refactoring and Total Biosynthesis of Fungal Natural Products	15
Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds	15
Creating RNA Specific C-to-U Editase from APOBEC3A by Separation of Its Activities on DNA and RNA Substrates	15
DNA Computing: NOT Logic Gates See the Light	15
GroovDB: A Database of Ligand-Inducible Transcription Factors	15
Photocontrol of Itaconic Acid Synthesis in Escherichia coli	15
Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii	15
Reservoir Computing Using DNA Oscillators	15
Reprogramming Escherichia coli Metabolism for Bioplastics Synthesis from Waste Cooking Oil	15
The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune Cells	15
Reprogramming T7RNA Polymerase in Escherichia coli Nissle 1917 under Specific Lac Operon for Efficient p-Coumaric Acid Production	15
Establishment of Green- and Red-Fluorescent Reporter Proteins Based on the Fluorescence-Activating and Absorption-Shifting Tag for Use in Acetogenic and Solventogenic Anaerobes	15
Switchable CAR-T Cells Outperformed Traditional Antibody-Redirected Therapeutics Targeting Breast Cancers	15
Engineering Transcription Factor XylS for Sensing Phthalic Acid and Terephthalic Acid: An Application for Enzyme Evolution	15
Engineering a CRISPRi Circuit for Autonomous Control of Metabolic Flux in Escherichia coli	15
Construction of Canthaxanthin-Producing Yeast by Combining Spatiotemporal Regulation and Pleiotropic Drug Resistance Engineering	15
Spatiotemporal Regulation of Astaxanthin Synthesis in S. cerevisiae	15
Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid	15
Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination	15
Synthesis of Ligustrazine from Acetaldehyde by a Combined Biological–Chemical Approach	15
Single Cell Characterization of a Synthetic Bacterial Clock with a Hybrid Feedback Loop Containing dCas9-sgRNA	15
Mapping of Nonhomologous End Joining-Mediated Integration Facilitates Genome-Scale Trackable Mutagenesis in Yarrowia lipolytica	15
Yeast Synthetic Minimal Biosensors for Evaluating Protein Production	15
Development of a Novel Cytokine Vehicle Using Filamentous Phage Display for Colorectal Cancer Treatment	15
Deciphering the Design Rules of Toehold-Gated sgRNA for Conditional Activation of Gene Expression and Protein Degradation in Mammalian Cells	15
Optimization of Pinocembrin Biosynthesis in Saccharomyces cerevisiae	14
Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective	14
Poly-γ-Glutamic Acid Production by Engineering a DegU Quorum-Sensing Circuit in Bacillus subtilis	14
Semisynthetic Macrocyclic Lipo-lanthipeptides Display Antimicrobial Activity Against Bacterial Pathogens	14
RoboMoClo: A Robotics-Assisted Modular Cloning Framework for Multiple Gene Assembly in Biofoundry	14
Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis	14
Solving 0–1 Integer Programming Problem Based on DNA Strand Displacement Reaction Network	14
Orthogonal Degron System for Controlled Protein Degradation in Cyanobacteria	14
Design of Synthetic Quorum Sensing Achieving Induction Timing-Independent Signal Stabilization for Dynamic Metabolic Engineering of E. coli	14
Macrolide Biosensor Optimization through Cellular Substrate Sequestration	14
Predicting Composition of Genetic Circuits with Resource Competition: Demand and Sensitivity	14
Multiplexed Assembly and Annotation of Synthetic Biology Constructs Using Long-Read Nanopore Sequencing	14
Electrogenetic Signal Transmission and Propagation in Coculture to Guide Production of a Small Molecule, Tyrosine	14
An Integrative Toolbox for Synthetic Biology in Rhodococcus	14
Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Chlorogenic Acid from Glucose	14
Engineered Attenuated Salmonella typhimurium Expressing Neoantigen Has Anticancer Effects	14