OOIR: Observatory of International Research

Papers

(The TQCC of ACS Synthetic Biology is 11. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-10-01 to 2025-10-01.)

Article	Citations
Directed Evolution of a SelB Variant that Does Not Require a Selenocysteine Insertion Sequence Element for Function	93
Synthetic Biology 2.0: The Dawn of a New Era	92
Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight	86
Stem Loop Mediated Transgene Modulation in Human T Cells	84
Discovering Content through Text Mining for a Synthetic Biology Knowledge System	80
Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes	79
Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit	78
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids	70
The Japan-UK Synthetic Biology Conference, Spring 2025: Strengthening Global Links to Engineer Biology	68
Efficient Mutasynthesis of “Non-Natural” Antitubercular Ilamycins with Low Cytotoxicity	67
Nano-Regulation of Gene Expression in Chlamydomonas reinhardtii: Harnessing AuNPs for Remotely Switchable Lipid Biosynthesis via Antisense Oligonucleotides	65
Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes	62
Metabolic Engineering of Clostridium tyrobutyricum for High-Yield n-Butanol Production by Increasing Intracellular Reducing Equivalent with NADPH-Dependent 3-Hydroxybutyryl-CoA Dehydroge	62
Predicting and Interpreting Protein Developability Via Transfer of Convolutional Sequence Representation	60
Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit	57
Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many	57
Recent Advances in the Biological Synthesis of l-Tyrosine Derivatives by Genetically Engineered Microbes	55
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis	55
A Logically Reversible Double Feynman Gate with Molecular Engineered Bacteria Arranged in an Artificial Neural Network-Type Architecture	54
Strategies for Improved Docosahexaenoic Acid Synthesis in Microalgae	53
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	51
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering	51
Issue Publication Information	49
Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing	49
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cI^antisense	49

Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens	48
A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage	46
Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds	46
Metabolic Engineering of Microbial Chassis Cells for d-Pantothenate Production: A Review	46
Cell-Free Protein Synthesis as a Method to Rapidly Screen Machine Learning-Generated Protease Variants	45
Lipid Modification and Membrane Localization of Proteins in Cell-Free System	45
Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis	44
LOICA: Integrating Models with Data for Genetic Network Design Automation	43
Engineered Mutants of a Marine Photosynthetic Purple Nonsulfur Bacterium with Increased Volumetric Productivity of Polyhydroxyalkanoate Bioplastics	43
Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis	43
Construction of an Engineered Escherichia coli with Efficient Chemotactic and Metabolizing Ability toward Tetrathionate	43
Bacteria Engineered to Produce Serotonin Modulate Host Intestinal Physiology	42
Hybrid Methylotrophic Pathway in Serratia marcescens for Sustainable Terpenoid Biosynthesis	41
Parameterized Computational Framework for the Description and Design of Genetic Circuits of Morphogenesis Based on Contact-Dependent Signaling and Changes in Cell–Cell Adhesion	41
Protease-Responsive Toolkit for Conditional Targeted Protein Degradation	40
Engineered l-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments	39
Actomyosin-Driven Division of a Synthetic Cell	39
Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida	38
Combining Chemical Catalysis with Enzymatic Steps for the Synthesis of the Artemisinin Precursor Dihydroartemisinic Acid	37
IWBDA 2022: Toward a Modular Future of Synthetic Biology Driven by Bio-Design Automation	37
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield	36
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles	36
An Autocatalytic Peptide Cyclase Improves Fidelity and Yield of Circular Peptides In Vivo and In Vitro	36
In VivoSampling of Intracellular Heterogeneity ofPseudomonas putidaEnables Multiobjective Optimization of Genetic Devices	36
Energy Aware Technology Mapping of Genetic Logic Circuits	36
LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning	35
Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose	35
Combinatorial Nonribosomal Peptide Synthetase Libraries Using the SEAM-Combi-OGAB Method	35
Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies	35
Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation	34
CRISPR-Based Regulation for High-Throughput Screening	34
An Arrayed CRISPR Screen Identifies Knockout Combinations Improving Antibody Productivity in HEK293 Cells	34
Zymo-Parts: A Golden Gate Modular Cloning Toolbox for Heterologous Gene Expression in Zymomonas mobilis	34
Issue Editorial Masthead	33
Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles	33
Issue Editorial Masthead	33
Hybrid Cas12a Variants with Relaxed PAM Requirements Expand Genome Editing Compatibility	33
Combinatorial Tuning of 5′UTR and N-Terminal Coding Sequences for Enhanced Recombinant Protein Expression in Corynebacterium glutamicum	32
Functional and Structural Roles of the Dimer Interface in the Activity and Stability of Clostridium butyricum 1,3-Propanediol Oxidoreductase	32
RoboMoClo: A Robotics-Assisted Modular Cloning Framework for Multiple Gene Assembly in Biofoundry	32
DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron	31
Emergent Bistable Switches from the Incoherent Feed-Forward Signaling of a Positive Feedback Loop	31
Modular Metabolic Engineering and Synthetic Coculture Strategies for the Production of Aromatic Compounds in Yeast	31
Spatiotemporal Gene Expression by a Genetic Circuit for Chemical Production in Escherichia coli	31
AutoBioTech─A Versatile Biofoundry for Automated Strain Engineering	31
Combating Infectious Diseases with Synthetic Biology	31
Directing Uphill Strand Displacement with an Engineered Superhelicase	31
RNA Polymerase-Mediated Stepwise RNA-Primed RNA Polymerization for Site-Specific Multiple Labeling into RNA: A Fluorescence Resonance Energy Transfer Probe Detects the Structural Change of an RNA G-Qu	30
RNA Aptamer-Mediated Gene Activation Systems for Inducible Transgene Expression in Animal Cells	30
Directed Design of Anti-Idiotypic Nanobody with Antibacterial Activity against Foodborne Staphylococcus aureus and Its Innovative Application in Green Immunoassay	30

CRISPR-Cas-Mediated Tethering Recruits the Yeast HMR Mating-Type Locus to the Nuclear Periphery but Fails to Silence Gene Expression	30
Harnessing the Endogenous Type I–F CRISPR/Cas System for Efficient Genome Engineering and Gene Repression in Pseudomonas chlororaphis LX24	30
Fine-Regulating the Carbon Flux of l-Isoleucine Producing Corynebacterium glutamicum WM001 for Efficient l-Threonine Production	30
CRISPR Diagnostics for Quantification and Rapid Diagnosis of Myotonic Dystrophy Type 1 Repeat Expansion Disorders	29
An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts	29
Pathway Refactoring for Efficient 7-Dehydrocholesterol Production in Saccharomyces cerevisiae	29
Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins in Saccharomyces cerevisiae	29
Characterization of Integrase and Excisionase Activity in a Cell-Free Protein Expression System Using a Modeling and Analysis Pipeline	29
Switchable Zinc(II)-Responsive Globular β-Sheet Peptide	29
Reducing Cellular Autolysis of Bacillus subtilis to Improve Keratinase Production	29
Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Cell-Free Transcription–Translation Platform	29
Engineered Acinetobacter baylyi ADP1-ISx Cells Are Sensitive DNA Biosensors for Antibiotic Resistance Genes and a Fungal Pathogen of Bats	29
Engineering a Synthetic Dopamine-Responsive Riboswitch for In Vitro Biosensing	28
Cheating the Cheater: Suppressing False-Positive Enrichment during Biosensor-Guided Biocatalyst Engineering	28
GPCR-FEX: A Fluoride-Based Selection System for Rapid GPCR Screening and Engineering	28
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae	28
High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis in Yarrowia lipolytica	28
Induced Neural Progenitor Specification from Human Pluripotent Stem Cells by a Refined Synthetic Notch Platform	28
RAGATH-Associated DNA Nuclease Assisted DNA Insertion in Corynebacterium glutamicum	28
De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii	27
Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles	27
Genetic Engineering of Bacteriophage K1F with Human Epidermal Growth Factor to Enhance Killing of Intracellular E. coli K1	27
Engineering Yeast Yarrowia lipolytica for Methanol Assimilation	27
Development of a Type I-E CRISPR-Based Programmable Repression System for Fine-Tuning Metabolic Flux toward D-Pantothenic Acid in Bacillus subtilis	26
Issue Publication Information	26
Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide	26
Issue Publication Information	26
Iron-Tannin Coating Reduces Clearance and Increases Tumor Colonization of Systemically Delivered Bacteria	26
Issue Publication Information	26
Engineering and Optimization of the Salidroside Production in Tobacco Cells by Reconstructed Biosynthetic Genes from Rhodiola rosea	25
Precision Transcriptome Editing	25
Functional Modification of Cyanobacterial Phycobiliprotein and Phycobilisomes through Bilin Metabolism Control	25
Light-Induced Nanobody-Mediated Targeted Protein Degradation for Metabolic Flux Control	25
An Integrative Strategy Enhancing Nanobody Thermostability via CDR Grafting, In Silico Mutagenesis Screening, and Multiplex Evaluation	25
Issue Publication Information	25
Hyperstable Synthetic Mini-Proteins as Effective Ligand Scaffolds	24
Expanding the Cell-Free Reporter Protein Toolbox by Employing a Split mNeonGreen System to Reduce Protein Synthesis Workload	24
pUdOs: Concise Plasmids for Bacterial and Mammalian Cells	24
Optically Controlled CRISPR-Cas9 and Cre Recombinase for Spatiotemporal Gene Editing: A Review	24
Engineering a Synthetic Escherichia coli Coculture for Compartmentalized de novo Biosynthesis of Isobutyl Butyrate from Mixed Sugars	24
A High-Throughput Colocalization Pipeline for Quantification of Mitochondrial Targeting across Different Protein Types	24
Precision Genome Engineering in Streptococcus suis Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology	24
Metabolic Engineering of Escherichia coli for Self-Induced Production of l-Isoleucine	24
Combinatorial PCR Method for Efficient, Selective Oligo Retrieval from Complex Oligo Pools	24
PASIV: A Pooled Approach-Based Workflow to Overcome Toxicity-Induced Design of Experiments Failures and Inefficiencies	24
Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform	24
Automated Construction of a Yeast-Based Multigene Library via Homologous Recombination in a Biofoundry Workflow	24
Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation	24
Harnessing Escherichia coli’s Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency	23
Highly Parallelized Construction of DNA from Low-Cost Oligonucleotide Mixtures Using Data-Optimized Assembly Design and Golden Gate	23
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts	23
Exploration of the Native Sucrose Operon Enables the Development of an Inducible T7 Expression System in Paenibacillus polymyxa	23
DNA Origami Nanostructure Detection and Yield Estimation Using Deep Learning	23
RNA-Based Sensor Systems for Affordable Diagnostics in the Age of Pandemics	23
ATP-Binding Cassette Exporter PDR11-Mediated Terpenoid Secretion in Engineered Yeast	23
HinZip: Combining Hin Recombinase and FosW to Mimic HD-Zip Plant Proteins	23
A Tunable and Expandable Transactivation System in Probiotic Yeast Saccharomyces boulardii	23
Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection Marker tetA^OPT	23
Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable	23
Endotoxin-Free Outer Membrane Vesicles for Safe and Modular Anticancer Immunotherapy	22
One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions	22
Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)	22
Implementation of a Novel Optogenetic Tool in Mammalian Cells Based on a Split T7 RNA Polymerase	22
Pseudotyping Bacteriophage P2 Tail Fibers to Extend the Host Range for Biomedical Applications	22
Modular and Flexible Molecular Device for Simultaneous Cytosine and Adenine Base Editing at Random Genomic Loci in Filamentous Fungi	22
Special Issue on Artificial Intelligence for Synthetic Biology	22
DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein Engineering	22
Enabling Biological Nitrogen Fixation for Cereal Crops in Fertilized Fields	22
Reconstruction of a Genome-Scale Metabolic Model of Scenedesmus obliquus and Its Application for Lipid Production under Three Trophic Modes	22
Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803	21
Engineering Escherichia coli for Perillyl Alcohol Production with Reduced Endogenous Dehydrogenation	21
Production of Caffeic Acid with Co-fermentation of Xylose and Glucose by Multi-modular Engineering in Candida glycerinogenes	21
A Layered Genetic Design Enables the Yeast Galactose Regulon to Respond to Cyanamide	21
Evaluating Apoptotic Gene Efficiency for CHO Culture Performance Using Targeted Integration	21
Discovery of Latent Cannabichromene Cyclase Activity in Marine Bacterial Flavoenzymes	21
Functional Deimmunization of Botulinum Neurotoxin Protease Domain via Computationally Driven Library Design and Ultrahigh-Throughput Screening	21
Correction to “Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation”	21
A Ubiquitous Chromatin Opening Element and DNA Demethylation Facilitate Doxycycline-Controlled Expression during Differentiation and in Transgenic Mice	21
Advances in Field Detection Based on CRISPR/Cas System	21
Amplification-Free COVID-19 Detection by Digital Droplet REVEALR	21

Engineered Stop and Go T7 RNA Polymerases	21
Versatile Design of Intracellular Protein-Responsive Translational Regulation System for Synthetic mRNA	21
Point-of-Care Peptide Hormone Production Enabled by Cell-Free Protein Synthesis	20
Engineering Microbial Consortium Biohybrid System to Efficiently Produce Electricity from Lignocellulose Biomass	20
Pooled CRISPR Interference Screening Identifies Crucial Transcription Factors in Gas-Fermenting Clostridium ljungdahlii	20
Construction of Canthaxanthin-Producing Yeast by Combining Spatiotemporal Regulation and Pleiotropic Drug Resistance Engineering	20
Tailoring Bacterial Cellulose through the CRISPR/Cas9-Mediated Gene Editing Tool in Komagataeibacter xylinus	20
Transcriptomics-Driven Engineering of Yarrowia lipolytica for Enhanced Fatty Acid Biosynthesis	20
Highly Reversible Tunable Thermal-Repressible Split-T7 RNA Polymerases (Thermal-T7RNAPs) for Dynamic Gene Regulation	20
Biosynthesis of the Functional Component Spermidine from Bacillus amyloliquefaciens by Iterative Integration Expression	20
Robust Synthetic Biology Toolkit to Advance Carboxysome Study and Redesign	20
Marine Biofilm Engineered to Produce Current in Response to Small Molecules	20
A Simple and Highly Sensitive Naked-Eye Analysis of EGFR 19del via CRISPR/Cas12a Triggered No-Nonspecific Nucleic Acid Amplification	20
An In Vitro Hybrid Biocatalytic System Enabled by a Combination of Surface-Displayed, Purified, and Cell-Free Expressed Enzymes	20
Synthetic Biology of Natural Products Engineering: Recent Advances Across the Discover–Design–Build–Test–Learn Cycle	20
Biomolecular Condensate-Based Artificial Organelle for Driving Compartmentalized Flux Control	20
Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene Biosynthesis	20
Metabolic Engineering of Candida glycerinogenes for Sustainable Production of Geraniol	20
Recombinant Protein Expression Chassis Library of Vibrio natriegens by Fine-Tuning the Expression of T7 RNA Polymerase	20
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast	19
Synthetic Homoserine Lactone Sensors for Gram-Positive Bacillus subtilis Using LuxR-Type Regulators	19
Unraveling the Constrained Cell Growth in Engineered Living Materials	19
Engineering Temperature-Powered Synthetic Multilayer Molecular Bioswitch for High-Level Pyruvate Derivative Production in Escherichia coli	19
Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems	19
Spatially Selective Electrochemical Cleavage of a Polymerase-Nucleotide Conjugate	19
HUHgle: An Interactive Substrate Design Tool for Covalent Protein-ssDNA Labeling Using HUH-Tags	19
Metabolic Engineering of Methanotrophic Bacteria for De Novo Production of Taxadiene from Methane	19
Issue Editorial Masthead	19
Genome-Wide CRISPR/Cas9 Screening Unveils a Novel Target ATF7IP–SETDB1 Complex for Enhancing Difficult-to-Express Protein Production	19
Issue Editorial Masthead	19
Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli	19
Conditional Control of Universal CAR T Cells by Cleavable OFF-Switch Adaptors	19
Issue Editorial Masthead	19
Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape	19
Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of l-Pipecolic Acid from Glucose	19
Imaging Method Using CRISPR/dCas9 and Engineered gRNA Scaffolds Can Perturb Replication Timing at the HSPA1 Locus	19
Low Resource Integrated Platform for Production and Analysis of Capped mRNA	19
Biosynthesis of Antimicrobial Ornithine-Containing Lacticin 481 Analogues by Use of a Combinatorial Biosynthetic Pathway in Escherichia coli	19
Designing Single-Component Optogenetic Membrane Recruitment Systems: The Rho-Family GTPase Signaling Toolbox	19
Evolutionary Stability Optimizer (ESO): A Novel Approach to Identify and Avoid Mutational Hotspots in DNA Sequences While Maintaining High Expression Levels	18
Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching	18
Channeling Anabolic Side Products toward the Production of Nonessential Metabolites: Stable Malate Production in Synechocystis sp. PCC6803	18
The Potential of Artificial Cells Functioning under In Situ Deep-Sea Conditions	18
Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation	18
Synthetic Platforms for Characterizing and Targeting of SARS-CoV-2 Genome Capping Enzymes	18
Efficient Autoinducible Expression of Recombinant Proteins via the DegSU Quorum Sensing System in a Robust Bacillus subtilis	18
Engineering a CRISPRi Circuit for Autonomous Control of Metabolic Flux in Escherichia coli	18
Optical Control of Phosphoinositide Binding: Rapid Activation of Subcellular Protein Translocation and Cell Signaling	18
Organellar Glue: A Molecular Tool to Artificially Control Chloroplast–Chloroplast Interactions	18
Engineering a Yeast Cell Factory to Sustainably Biosynthesize Parthenolide	18
Issue Editorial Masthead	18
A Simple and Effective Strategy for the Development of Robust Promoter-Centric Gene Expression Tools	18
Genetic Code Expansion in Pseudomonas putida KT2440	18
Microbial Synthesis of Nucleosides: Advances and Prospects	18
A Decade of SBOL Visual: Growing Adoption of a Diagram Standard for Engineering Biology	18
Statistical Analysis and Tokenization of Epitopes to Construct Artificial Neoepitope Libraries	18
Issue Publication Information	18
Correction to “Hyperaccurate Ribosomes for Improved Genetic Code Reprogramming”	18
Synthetic Ecosystems: From the Test Tube to the Biosphere	18
Simulations Reveal High Efficiency and Confinement of a Population Suppression CRISPR Toxin-Antidote Gene Drive	18
Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans	18
Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium	17
Molecular Mechanism of the Cytosine CRISPR Base Editing Process and the Roles of Translesion DNA Polymerases	17
GroovDB: A Database of Ligand-Inducible Transcription Factors	17
Investigating the Specificity of the Dehydration and Cyclization Reactions in Engineered Lanthipeptides by Synechococcal SyncM	17
Modular Design of a Copper Ion Biosensor Based on the MAPK Signaling Pathway in Saccharomyces cerevisiae	17
Transfer RNA Synthesis-Coupled Translation and DNA Replication in a Reconstituted Transcription/Translation System	17
Antibiotics-Free Steady Bioproduction of Valuable Chemicals from Organic Wastes by Engineered Vibrio natriegens through Targeted Gene Integration	17
Rational Engineering of Escherichia coli Nissle 1917 as Live Biotherapeutic to Degrade Uremic Toxin Precursors	17
Identifying Conditions for Protein Synthesis Inside Giant Vesicles Using Microfluidics toward Standardized Artificial Cell Production	17
Sense-and-Respond Payload Delivery Using a Novel Antigen-Inducible Promoter Improves Suboptimal CAR-T Activation	17
Introduction to Engineering Biology: A Conceptual Framework for Teaching Synthetic Biology	17
Engineering a Controllable Targeted Protein Degradation System and a Derived OR-GATE-Type Inducible Gene Expression System in Synechococcus elongatus PCC 7942	17
Conditionally Activated Cross-Linked crRNAs for CRISPR/Cas12a Based Nucleic Acid Detection	17
Robust Inducible Gene Expression in Intracellular Listeria monocytogenes In Vivo	17
Logic Circuits Based on 2A Peptide Sequences in the Yeast Saccharomyces cerevisiae	17
Reliable Genomic Integration Sites in Pseudomonas putida Identified by Two-Dimensional Transcriptome Analysis	17
Biotin-Independent Saccharomyces cerevisiae with Enhanced Growth: Engineering an Acetyl-CoA Carboxylase Bypass	17
Combinatorial Engineering of Escherichia coli for Enhancing 3-Fucosyllactose Production	17
Sequence-Based Searching for SynBioHub Using VSEARCH	16
Issue Publication Information	16
Mass Spectrometric Screening for Improving Enzymatic Conversion of Formaldehyde into C2 and C3 Products	16
Optimization of Campesterol-Producing Yeast Strains as a Feasible Platform for the Functional Reconstitution of Plant Membrane-Bound Enzymes	16
Establishing an Artificial Pathway for the Biosynthesis of Octopamine and Synephrine	16
Issue Editorial Masthead	16
Bacterial Living Therapeutics with Engineered Protein Secretion Circuits to Eliminate Breast Cancer Cells	16
Development and Application of an ACQUIRE Method for Direct Cloning of Superlarge Biosynthetic Gene Cluster	16
RNA Polymerase III Promoters Compatible with CRISPR Gene Regulation in Saccharomyces cerevisiae	16
Bioluminescent Genetically Encoded Glutamate Indicators for Molecular Imaging of Neuronal Activity	16
Quantitative Analysis of γ-Aminobutyric Acid by Combined Cell-Free Protein Synthesis and Transamination Reactions	16
Characterization and Engineering of a Novel Miniature Eubacterium siraeum CRISPR-Cas12f System	16
Red Light Responsive Cre Recombinase for Bacterial Optogenetics	16
Directed Evolution of Herbicide Biosensors in a Fluorescence-Activated Cell-Sorting-Compatible Yeast Two-Hybrid Platform	16
Construction of a Protein Crystalline Inclusion-Based Enzyme Immobilization System for Biosynthesis of PAPS from ATP and Sulfate	16
Optimization of Hydrogenobyrinic Acid Synthesis in a Cell-Free Multienzyme Reaction by Novel S-Adenosyl-methionine Regeneration	16