ACS Synthetic Biology

Article	Citations
Robotic DNA Nanostructures	102
Discovery of Novel Gain-of-Function Mutations Guided by Structure-Based Deep Learning	80
An Automated Model Test System for Systematic Development and Improvement of Gene Expression Models	79
Biosystems Design by Machine Learning	76
Compartmentalizing Cell-Free Systems: Toward Creating Life-Like Artificial Cells and Beyond	70
Transcription-Factor-based Biosensor Engineering for Applications in Synthetic Biology	68
Signal Peptides Generated by Attention-Based Neural Networks	63
Isothermal SARS-CoV-2 Diagnostics: Tools for Enabling Distributed Pandemic Testing as a Means of Supporting Safe Reopenings	62
Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput	55
Engineering Yarrowia lipolytica as a Chassis for De Novo Synthesis of Five Aromatic-Derived Natural Products and Chemicals	55
Application of CRISPR-Cas12a Enhanced Fluorescence Assay Coupled with Nucleic Acid Amplification for the Sensitive Detection of African Swine Fever Virus	55
Statistical Design of Experiments for Synthetic Biology	53
Multicopy Chromosomal Integration Using CRISPR-Associated Transposases	53
Engineering Wired Life: Synthetic Biology for Electroactive Bacteria	52
Improved Production of Malic Acid in Aspergillus niger by Abolishing Citric Acid Accumulation and Enhancing Glycolytic Flux	51
Systematic Quantification of Sequence and Structural Determinants Controlling mRNA stability in Bacterial Operons	50
Synthetic Glycobiology: Parts, Systems, and Applications	50
Bio-SCAN: A CRISPR/dCas9-Based Lateral Flow Assay for Rapid, Specific, and Sensitive Detection of SARS-CoV-2	49
A Unified Dynamic Programming Framework for the Analysis of Interacting Nucleic Acid Strands: Enhanced Models, Scalability, and Speed	48
CRISPR-Mediated Activation of Biosynthetic Gene Clusters for Bioactive Molecule Discovery in Filamentous Fungi	47
CRISPRi-dCas12a: A dCas12a-Mediated CRISPR Interference for Repression of Multiple Genes and Metabolic Engineering in Cyanobacteria	46
Bacteriophage Inspired Growth-Decoupled Recombinant Protein Production in Escherichia coli	45
CRISPR-Cas12a-Assisted Multicolor Biosensor for Semiquantitative Point-of-Use Testing of the Nopaline Synthase Terminator in Genetically Modified Crops by Unaided Eyes	43
Optogenetic Amplification Circuits for Light-Induced Metabolic Control	43
Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production	43

Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles	42
Enabling Biological Nitrogen Fixation for Cereal Crops in Fertilized Fields	42
A Dual-Plasmid CRISPR/Cas System for Mycotoxin Elimination in Polykaryotic Industrial Fungi	42
Transcription and Translation in Cytomimetic Protocells Perform Most Efficiently at Distinct Macromolecular Crowding Conditions	41
Bottom-Up Construction of a Minimal System for Cellular Respiration and Energy Regeneration	41
A Whole-Cell Biosensor for Point-of-Care Detection of Waterborne Bacterial Pathogens	40
Synthetic Virus-like Particles for Glutathione Biosynthesis	40
Cell-Free Gene Expression Dynamics in Synthetic Cell Populations	39
Multicopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells	39
Rational Promoter Engineering Enables Robust Terpene Production in Microalgae	38
Automated Continuous Evolution of Proteins in Vivo	38
CRISPR-Cas13 System as a Promising and Versatile Tool for Cancer Diagnosis, Therapy, and Research	38
A Chemical Switch System to Modulate Chimeric Antigen Receptor T Cell Activity through Proteolysis-Targeting Chimaera Technology	37
Development of Novel Riboswitches for Synthetic Biology in the Green Alga Chlamydomonas	37
Rewiring Central Carbon Metabolism Ensures Increased Provision of Acetyl-CoA and NADPH Required for 3-OH-Propionic Acid Production	36
CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis	36
Simultaneous Improvement of Limonene Production and Tolerance in Yarrowia lipolytica through Tolerance Engineering and Evolutionary Engineering	36
Modular Synthetic Biology Toolkit for Filamentous Fungi	35
High-Titer De Novo Biosynthesis of the Predominant Human Milk Oligosaccharide 2′-Fucosyllactose from Sucrose in Escherichia coli	35
Design and Characterization of Rapid Optogenetic Circuits for Dynamic Control in Yeast Metabolic Engineering	34
Updated ATLAS of Biochemistry with New Metabolites and Improved Enzyme Prediction Power	34
Efficient Biosynthesis of (2S)-Eriodictyol from (2S)-Naringenin in Saccharomyces cerevisiae through a Combination of Promoter Adjustment and Directed Evolution	34
A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics	34
Encapsulin Nanocontainers as Versatile Scaffolds for the Development of Artificial Metabolons	34
A User’s Guide to Golden Gate Cloning Methods and Standards	34
A Small-Molecule-Responsive Riboswitch Enables Conditional Induction of Viral Vector-Mediated Gene Expression in Mice	33
In Vivo Feedback Control of an Antithetic Molecular-Titration Motif in Escherichia coli Using Microfluidics	33
Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917	32
High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae	32
Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design	32
3D Bioprinting in Skin Related Research: Recent Achievements and Application Perspectives	32
Targeted Diversification in the S. cerevisiae Genome with CRISPR-Guided DNA Polymerase I	32
Improving the Heterologous Production of Fungal Peroxygenases through an Episomal Pichia pastoris Promoter and Signal Peptide Shuffling System	32
Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris	32
In Situ Biomanufacturing of Small Molecules in the Mammalian Gut by Probiotic Saccharomyces boulardii	32
Blue-Light-Switchable Bacterial Cell–Cell Adhesions Enable the Control of Multicellular Bacterial Communities	32
Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation	32
Kinetics of RNA and RNA:DNA Hybrid Strand Displacement	31
Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network	31
Prime Editing Guide RNA Design Automation Using PINE-CONE	31
Evolving a Generalist Biosensor for Bicyclic Monoterpenes	30
Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems	30
Functional Identification of Two Types of Carotene Hydroxylases from the Green Alga Dunaliella bardawil Rich in Lutein	30
MiniBacillus PG10 as a Convenient and Effective Production Host for Lantibiotics	30
Facilitating Protein Expression with Portable 5′-UTR Secondary Structures in Bacillus licheniformis	30
CRISPR/Cas-Based Genome Editing for Human Gut Commensal Bacteroides Species	30
Optogenetic Control of Microbial Consortia Populations for Chemical Production	30
Synthetic Biology Toolkit for Marker-Less Integration of Multigene Pathways into Pichia pastoris via CRISPR/Cas9	30
Genome-Wide CRISPRi-Based Identification of Targets for Decoupling Growth from Production	30
Engineering Critical Enzymes and Pathways for Improved Triterpenoid Biosynthesis in Yeast	30

A Golden-Gate Based Cloning Toolkit to Build Violacein Pathway Libraries in Yarrowia lipolytica	29
Metabolic Engineering of Escherichia coli for De Novo Production of 1,5-Pentanediol from Glucose	29
Engineered Microbial Routes for Human Milk Oligosaccharides Synthesis	29
Programmed Allelic Mutagenesis of a DNA Polymerase with Single Amino Acid Resolution	29
Rational Design of CRISPR/Cas12a-RPA Based One-Pot COVID-19 Detection with Design of Experiments	29
Metabolic Engineering of Gas-Fermenting Clostridium ljungdahlii for Efficient Co-production of Isopropanol, 3-Hydroxybutyrate, and Ethanol	28
Reprogramming Acetogenic Bacteria with CRISPR-Targeted Base Editing via Deamination	28
Programmable Fusion and Differentiation of Synthetic Minimal Cells	28
A Protein-Engineered, Enhanced Yeast Display Platform for Rapid Evolution of Challenging Targets	28
Systematically Engineered Fatty Acid Catabolite Pathway for the Production of (2S)-Naringenin in Saccharomyces cerevisiae	28
Functional Integration of Two CYP450 Genes Involved in Biosynthesis of Tanshinones for Improved Diterpenoid Production by Synthetic Biology	28
Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production	28
Expanding Toolbox for Genes Expression of Yarrowia lipolytica to Include Novel Inducible, Repressible, and Hybrid Promoters	27
Chemical Controllable Gene Drive in Drosophila	26
Engineering Prokaryotic Transcriptional Activator XylR as a Xylose-Inducible Biosensor for Transcription Activation in Yeast	26
Mycoplasma pneumoniae Genome Editing Based on Oligo Recombineering and Cas9-Mediated Counterselection	26
De Novo Biosynthesis of Multiple Pinocembrin Derivatives in Saccharomyces cerevisiae	26
Dynamic Control of 4-Hydroxyisoleucine Biosynthesis by Modified l-Isoleucine Biosensor in Recombinant Corynebacterium glutamicum	26
Label-Free Detection of Transgenic Crops Using an Isothermal Amplification Reporting CRISPR/Cas12 Assay	26
A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines	26
Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide	26
Biotechnological Production of 2′-Fucosyllactose: A Prevalent Fucosylated Human Milk Oligosaccharide	26
A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology	26
RPA/CRISPR/Cas12a-Based On-Site and Rapid Nucleic Acid Detection of Toxoplasma gondii in the Environment	25
Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast	25
High-Throughput Screening for Substrate Specificity-Adapted Mutants of the Nisin Dehydratase NisB	25
Metabolic Engineering of Saccharomyces cerevisiae for Rosmarinic Acid Production	25
Building a Versatile Protein Production Platform Using Engineered Trichoderma reesei	25
Precise Prediction of Promoter Strength Based on a De Novo Synthetic Promoter Library Coupled with Machine Learning	25
Thermal Control of Engineered T-cells	25
A Reversibly Induced CRISPRi System Targeting Photosystem II in the Cyanobacterium Synechocystis sp. PCC 6803	25
Highly Efficient Production of l-Histidine from Glucose by Metabolically Engineered Escherichia coli	25
YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants	25
Engineering the Lipid and Fatty Acid Metabolism in Yarrowia lipolytica for Sustainable Production of High Oleic Oils	24
Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment	24
De Novo Engineering of Corynebacterium glutamicum for l-Proline Production	24
Constructing Cell-Free Expression Systems for Low-Cost Access	24
Evolution of Functionally Enhanced α-l-Threofuranosyl Nucleic Acid Aptamers	24
Protecting Linear DNA Templates in Cell-Free Expression Systems from Diverse Bacteria	24
A Single Cas9-VPR Nuclease for Simultaneous Gene Activation, Repression, and Editing in Saccharomyces cerevisiae	24
Engineering Stable Pseudomonas putida S12 by CRISPR for 2,5-Furandicarboxylic Acid (FDCA) Production	24
Recognition of DNA Target Formulations by CRISPR-Cas12a Using a dsDNA Reporter	24
Membrane Augmented Cell-Free Systems: A New Frontier in Biotechnology	23
Increased Lipid Production in Yarrowia lipolytica from Acetate through Metabolic Engineering and Cosubstrate Fermentation	23
Continuous Cell-Free Replication and Evolution of Artificial Genomic DNA in a Compartmentalized Gene Expression System	23
The Bacillus subtilis Minimal Genome Compendium	23
Engineering Carboxylic Acid Reductase (CAR) through a Whole-Cell Growth-Coupled NADPH Recycling Strategy	23
Establishment of a Biosensor-based High-Throughput Screening Platform for Tryptophan Overproduction	23
A Library of Tunable, Portable, and Inducer-Free Promoters Derived from Cyanobacteria	23
Efficient Production of 2′-Fucosyllactose from l-Fucose via Self-Assembling Multienzyme Complexes in Engineered Escherichia coli	23
Translation Related Factors Improve the Productivity of a Streptomyces-Based Cell-Free Protein Synthesis System	23
Optogenetic Control of the BMP Signaling Pathway	22
Improve the Biosynthesis of Baicalein and Scutellarein via Manufacturing Self-Assembly Enzyme Reactor In Vivo	22
Comprehensive Analysis of Genomic Safe Harbors as Target Sites for Stable Expression of the Heterologous Gene in HEK293 Cells	22
Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in E. coli	22
Reading and Writing Digital Information in TNA	22
Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host	22
Cheetah: A Computational Toolkit for Cybergenetic Control	22
Low-Bias Manipulation of DNA Oligo Pool for Robust Data Storage	22
Cell Synchronization Enhances Nuclear Transformation and Genome Editing via Cas9 Enabling Homologous Recombination in Chlamydomonas reinhardtii	22
Biosynthesis of a Therapeutically Important Nicotinamide Mononucleotide through a Phosphoribosyl Pyrophosphate Synthetase 1 and 2 Engineered Strain of Escherichia coli	22
Tuning Extracellular Electron Transfer by Shewanella oneidensis Using Transcriptional Logic Gates	22
A Novel Biosynthetic Pathway for the Production of Acrylic Acid through β-Alanine Route in Escherichia coli	22
Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus	21
Aptamer-Mediated Control of Polyadenylation for Gene Expression Regulation in Mammalian Cells	21
High-Performance Allosteric Conditional Guide RNAs for Mammalian Cell-Selective Regulation of CRISPR/Cas	21
Curli-Mediated Self-Assembly of a Fibrous Protein Scaffold for Hydroxyapatite Mineralization	21
Optimization of a p-Coumaric Acid Biosensor System for Versatile Dynamic Performance	21
Engineering Yeast Yarrowia lipolytica for Methanol Assimilation	21
Directed Evolution of Ornithine Cyclodeaminase Using an EvolvR-Based Growth-Coupling Strategy for Efficient Biosynthesis of l-Proline	21
Genetic Circuit Dynamics: Hazard and Glitch Analysis	21
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries	21
evSeq: Cost-Effective Amplicon Sequencing of Every Variant in a Protein Library	20
Standardization of Synthetic Biology Tools and Assembly Methods for Saccharomyces cerevisiae and Emerging Yeast Species	20
Rational Design of Novel Fluorescent Enzyme Biosensors for Direct Detection of Strigolactones	20
Cell Catalysis of Citrate to Itaconate by Engineered Halomonas bluephagenesis	20
Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom	20
Artificial ER-Derived Vesicles as Synthetic Organelles for in Vivo Compartmentalization of Biochemical Pathways	20
Constructing a Novel Biosynthetic Pathway for the Production of Glycolate from Glycerol in Escherichia coli	19
Efficient Biosynthesis of Low-Molecular-Weight Poly-γ-glutamic Acid Based on Stereochemistry Regulation in Bacillus amyloliquefaciens	19

Systems Metabolic Engineering of Escherichia coli Coculture for De Novo Production of Genistein	19
Construction of an l-Tyrosine Chassis in Pichia pastoris Enhances Aromatic Secondary Metabolite Production from Glycerol	19
Bringing Light into Cell-Free Expression	19
Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13	19
One-Pot Synthesis of Adipic Acid from Guaiacol in Escherichia coli	19
Novel Prokaryotic CRISPR-Cas12a-Based Tool for Programmable Transcriptional Activation and Repression	19
Identification of a Strong Quorum Sensing- and Thermo-Regulated Promoter for the Biosynthesis of a New Metabolite Pesticide Phenazine-1-carboxamide in Pseudomonas strain PA1201	19
DIVERSIFY: A Fungal Multispecies Gene Expression Platform	19
Engineering E. coli for Magnetic Control and the Spatial Localization of Functions	19
Multicomponent Microscale Biosynthesis of Unnatural Cyanobacterial Indole Alkaloids	19
CRISPRi-Based Dynamic Regulation of Hydrolase for the Synthesis of Poly-γ-Glutamic Acid with Variable Molecular Weights	18
Combining CRISPR–Cpf1 and Recombineering Facilitates Fast and Efficient Genome Editing in Escherichia coli	18
An Aldolase-Based New Pathway for Bioconversion of Formaldehyde and Ethanol into 1,3-Propanediol in Escherichia coli	18
Microbial Synthesis of Human-Hormone Melatonin at Gram Scales	18
The Marburg Collection: A Golden Gate DNA Assembly Framework for Synthetic Biology Applications in Vibrio natriegens	18
Engineering the Reductive TCA Pathway to Dynamically Regulate the Biosynthesis of Adipic Acid in Escherichia coli	18
Evolving Small-Molecule Biosensors with Improved Performance and Reprogrammed Ligand Preference Using OrthoRep	18
Optogenetic Control of Heterologous Metabolism in E. coli	18
Automated Rational Strain Construction Based on High-Throughput Conjugation	18
Construction and Characterization of a Gradient Strength Promoter Library for Fine-Tuned Gene Expression in Bacillus licheniformis	18
Overcoming the Challenges of Megabase-Sized Plasmid Construction in Escherichia coli	18
Engineering Antisense Oligonucleotides as Antibacterial Agents That Target FMN Riboswitches and Inhibit the Growth of Staphylococcus aureus, Listeria monocytogenes, and Escherichia co	18
Two Completely Orthogonal Quorum Sensing Systems with Self-Produced Autoinducers Enable Automatic Delayed Cascade Control	18
CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus Glarea lozoyensis and Its Application in Manipulating gloF	17
Design and Reconstruction of Regulatory Parts for Fast-Growing Vibrio natriegens Synthetic Biology	17
Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters	17
High-Yielding Terpene-Based Biofuel Production in Rhodobacter capsulatus	17
Distributed Implementation of Boolean Functions by Transcriptional Synthetic Circuits	17
Decoupling Protein Production from Cell Growth Enhances the Site-Specific Incorporation of Noncanonical Amino Acids in E. coli	17
High-Titer Production of Olivetolic Acid and Analogs in Engineered Fungal Host Using a Nonplant Biosynthetic Pathway	17
Functional Comparison of Laboratory-Evolved XNA Polymerases for Synthetic Biology	17
Glycosylation Modification Enhances (2S)-Naringenin Production in Saccharomyces cerevisiae	17
A Novel and Efficient Genome Editing Tool Assisted by CRISPR-Cas12a/Cpf1 for Pichia pastoris	17
Efficient Production of 1,3-Propanediol from Diverse Carbohydrates via a Non-natural Pathway Using 3-Hydroxypropionic Acid as an Intermediate	17
Caged Activators of Artificial Allosteric Protein Biosensors	17
Application of Cell-Free Protein Synthesis System for the Biosynthesis of l-Theanine	17
Rerouting Fluxes of the Central Carbon Metabolism and Relieving Mechanism-Based Inactivation of l-Aspartate-α-decarboxylase for Fermentative Production of β-Alanine in Escherichia coli	17
SCRaMbLEing of a Synthetic Yeast Chromosome with Clustered Essential Genes Reveals Synthetic Lethal Interactions	17
Heterologous Expression of Mersacidin in Escherichia coli Elucidates the Mode of Leader Processing	17
Sequence Preference and Initiator Promiscuity for De Novo DNA Synthesis by Terminal Deoxynucleotidyl Transferase	17
Resveratrol Production from Hydrothermally Pretreated Eucalyptus Wood Using Recombinant Industrial Saccharomyces cerevisiae Strains	17
FlopR: An Open Source Software Package for Calibration and Normalization of Plate Reader and Flow Cytometry Data	17
Quantitative Yeast–Yeast Two Hybrid for the Discovery and Binding Affinity Estimation of Protein–Protein Interactions	17
A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae	17
Emergent Biological Endurance Depends on Extracellular Matrix Composition of Three-Dimensionally Printed Escherichia coli Biofilms	17
Repurposing the Endogenous Type I-E CRISPR/Cas System for Gene Repression in Gluconobacter oxydans WSH-003	17
Rational Design of Flavonoid Production Routes Using Combinatorial and Precursor-Directed Biosynthesis	16
Fine-Tuning Multi-Gene Clusters via Well-Characterized Gene Expression Regulatory Elements: Case Study of the Arginine Synthesis Pathway in C. glutamicum	16
Genome Engineering of the Fast-Growing Mycoplasma feriruminatoris toward a Live Vaccine Chassis	16
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts	16
Engineering a Central Carbon Metabolism Pathway to Increase the Intracellular Acetyl-CoA Pool in Synechocystis sp. PCC 6803 Grown under Photomixotrophic Conditions	16
Optimization of Heavy Metal Sensors Based on Transcription Factors and Cell-Free Expression Systems	16
Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis	16
De Novo Production of Plant 4′-Deoxyflavones Baicalein and Oroxylin A from Ethanol in Crabtree-Negative Yeast	16
Bioremediation of Industrial Pollutants by Insects Expressing a Fungal Laccase	16
Characterization of a Group of UDP-Glycosyltransferases Involved in the Biosynthesis of Triterpenoid Saponins of Panax notoginseng	16
A Sensitive and Specific Fluorescent RT-LAMP Assay for SARS-CoV-2 Detection in Clinical Samples	16
Engineering Strategies to Overcome the Stability–Function Trade-Off in Proteins	16
Saccharomyces cerevisiae Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins	16
Bioengineering Self-Organizing Signaling Centers to Control Embryoid Body Pattern Elaboration	16
Synergistic Metabolism of Glucose and Formate Increases the Yield of Short-Chain Organic Acids in Escherichia coli	16
Recombinant Protein Stability in Cyanobacteria	16
Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules	16
Multiplex Generation, Tracking, and Functional Screening of Substitution Mutants Using a CRISPR/Retron System	16
Direct Comparison of d-DNA and l-DNA Strand-Displacement Reactions in Living Mammalian Cells	16
Optogenetics in Sinorhizobium meliloti Enables Spatial Control of Exopolysaccharide Production and Biofilm Structure	16
Engineering T4 Bacteriophage for In Vivo Display by Type V CRISPR-Cas Genome Editing	15
Engineering of a Biosensor in Response to Malate in Bacillus licheniformis	15
Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli	15
Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuits	15
Regulatory Part Engineering for High-Yield Protein Synthesis in an All-Streptomyces-Based Cell-Free Expression System	15
Orthogonal Blue and Red Light Controlled Cell–Cell Adhesions Enable Sorting-out in Multicellular Structures	15
Transcript Barcoding Illuminates the Expression Level of Synthetic Constructs in E. coli Nissle Residing in the Mammalian Gut	15
Metabolic Engineering of Aspartic Acid Supply Modules for Enhanced Production of Bacitracin in Bacillus licheniformis	15
Applications of CRISPR in a Microbial Cell Factory: From Genome Reconstruction to Metabolic Network Reprogramming	15
Recruiting a Phosphite Dehydrogenase/Formamidase-Driven Antimicrobial Contamination System in Bacillus subtilis for Nonsterilized Fermentation of Acetoin	15
A Lambda Red and FLP/FRT-Mediated Site-Specific Recombination System in Komagataeibacter xylinus and Its Application to Enhance the Productivity of Bacterial Cellulose	15
GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants	15
Efficient Synthesis of Phycocyanobilin by Combinatorial Metabolic Engineering in Escherichia coli	15
Targeting glmS Ribozyme with Chimeric Antisense Oligonucleotides for Antibacterial Drug Development	15
Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites	15
Living Therapeutics: The Next Frontier of Precision Medicine	15
Engineering Yarrowia lipolytica to Produce Tailored Chain-Length Fatty Acids and Their Derivatives	15
A Computational Framework for Identifying Promoter Sequences in Nonmodel Organisms Using RNA-seq Data Sets	15
Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity	15
Rational Design of a Split Flavin-Based Fluorescent Reporter	15
A Biosensor for Detection of Indole Metabolites	15
Trade-Offs in Biosensor Optimization for Dynamic Pathway Engineering	15
Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida	15
CRISPR-Based Diagnostics: Challenges and Potential Solutions toward Point-of-Care Applications	15
Development of a Highly Efficient Copper-Inducible GAL Regulation System (CuIGR) in Saccharomyces cerevisiae	15
Multidimensional Characterization of Parts Enhances Modeling Accuracy in Genetic Circuits	15
Single Cell Characterization of a Synthetic Bacterial Clock with a Hybrid Feedback Loop Containing dCas9-sgRNA	14
Creating RNA Specific C-to-U Editase from APOBEC3A by Separation of Its Activities on DNA and RNA Substrates	14
Actomyosin-Driven Division of a Synthetic Cell	14