OOIR: Observatory of International Research

Papers

(The TQCC of ACS Synthetic Biology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-02-01 to 2025-02-01.)

Article	Citations
Generation of a Live Attenuated Influenza A Vaccine Using Chemical-Triggered Intein	84
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cI^antisense	64
Issue Editorial Masthead	63
ScEnSor Kit for Saccharomyces cerevisiae Engineering and Biosensor-Driven Investigation of the Intracellular Environment	61
Metabolic Tug-of-War between Glycolysis and Translation Revealed by Biochemical Reconstitution	59
Adenine Base Editing System for Pseudomonas and Prediction Workflow for Protein Dysfunction via ABE	53
PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H₂O₂ as Signal Integrator	53
A Computational Metabolic Model for Engineered Production of Resveratrol in Escherichia coli	52
Validation of Cell-Free Protein Synthesis Aboard the International Space Station	50
Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit	49
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries	48
Development of the Static and Dynamic Gene Expression Regulation Toolkit in Pseudomonas chlororaphis	47
Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many	45
The VEGAS Platform Is Unsuitable for Mammalian Directed Evolution	44
Interaction between the Anchoring Domain of A-Kinase Anchoring Proteins and the Dimerization and Docking Domain of Protein Kinase A: A Potent Tool for Synthetic Biology	43
A Biological OR(XNOR) Logic Gate Couples Carbon Source and Transgene Expression Switching in a Komagataella phaffii (Pichia pastoris) Strain Co-producing Process-Enhancing Lipase and a V	42
Issue Publication Information	42
Optical Control of Cell Signaling with Red/Far-Red Light-Responsive Optogenetic Tools in Caenorhabditis elegans	42
Issue Publication Information	41
Revolutionizing Point-of-Care Diagnostics via CRISPR Systems	41
Performance Prediction of Fundamental Transcriptional Programs	40
Inducible Synthetic Growth Regulation Using the ClpXP Proteasome Enhances cis,cis-Muconic Acid and Glycolic Acid Yields in Saccharomyces cerevisiae	40
Coproduction of Phase-Separated Carotenoids and β-Farnesene as a Yeast Biomass Valorization Strategy	39
DNA Computing: NOT Logic Gates See the Light	39
Plasmid Crosstalk in Cell-Free Expression Systems	39

Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit	39
Motility of Synthetic Cells from Engineered Lipids	39
Issue Editorial Masthead	38
Standard Intein Gene Expression Ramps (SIGER) for Protein-Independent Expression Control	37
Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins	37
New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference	37
Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida	36
Issue Editorial Masthead	35
Issue Editorial Masthead	35
Issue Publication Information	35
Issue Editorial Masthead	34
Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis	33
Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis	32
De Novo Production of Hydroxytyrosol by Saccharomyces cerevisiae–Escherichia coli Coculture Engineering	32
Transcription-Associated Fluorescence-Activated Droplet Sorting for Di-rhamnolipid Hyperproducers	32
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids	32
Reduction of the Bacterial Genome by Transposon-Mediated Random Deletion	31
Experimental and Analytical Approaches for Improving the Resolution of Randomly Barcoded Transposon Insertion Sequencing (RB-TnSeq) Studies	30
Directed Evolution of Acoustic Reporter Genes Using High-Throughput Acoustic Screening	30
Phage Enzyme-Assisted Direct In Vivo DNA Assembly in Multiple Microorganisms	30
Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight	29
De Novo Biosynthesis of the Anticancer Compound Euphol in Saccharomyces cerevisiae	29
Context-Aware Technology Mapping in Genetic Design Automation	29
A Computational Framework for Identifying Promoter Sequences in Nonmodel Organisms Using RNA-seq Data Sets	29
Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly	28
Evaluating the Contribution of Model Complexity in Predicting Robustness in Synthetic Genetic Circuits	28
Integrating Deep Learning and Synthetic Biology: A Co-Design Approach for Enhancing Gene Expression via N-Terminal Coding Sequences	28
Engineering of a TrpR-Based Biosensor for Altered Dynamic Range and Ligand Preference	27
Plasmid-Stabilizing Strains for Antibiotic-Free Chemical Fermentation	27
LOICA: Integrating Models with Data for Genetic Network Design Automation	27
Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose	27
A Hitchhiker’s Guide to Supplying Enzymatic Reducing Power into Synthetic Cells	27
MetJ-Based Mutually Interfering SAM-ON/SAM-OFF Biosensors	27
Tunable T7 Promoter Orthogonality on T7RNAP for cis-Aconitate Decarboxylase Evolution via Base Editor and Screening from Itaconic Acid Biosensor	27
Characterizing and Tailoring the Substrate Profile of a γ-Glutamyltransferase Variant	27
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	27
Ribosomal Protein S1 Improves the Protein Yield of an In Vitro Reconstituted Cell-Free Translation System	27
Multiple Rounds of In Vivo Random Mutagenesis and Selection in Vibrio natriegens Result in Substantial Increases in REE Binding Capacity	26
A Sirtuin-Dependent T7 RNA Polymerase Variant	26
Yeast Metabolic Engineering for Biosynthesis of Caffeic Acid-Derived Phenethyl Ester and Phenethyl Amide	26
GENETTA: a Network-Based Tool for the Analysis of Complex Genetic Designs	26
Coupling High-Throughput and Targeted Screening for Identification of Nonobvious Metabolic Engineering Targets	25
Synthetic Biology 2.0: The Dawn of a New Era	25
A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage	25
Sustainable Synthesis of Novel Green-Based Nanoparticles for Therapeutic Interventions and Environmental Remediation	24
Issue Publication Information	24
Cowpea NAC Transcription Factors Positively Regulate Cellular Stress Response and Balance Energy Metabolism in Yeast via Reprogramming of Biosynthetic Pathways	24
Protease-Responsive Toolkit for Conditional Targeted Protein Degradation	24
Structural Determinants for Light-Dependent Membrane Binding of a Photoswitchable Polybasic Domain	24
Pipetting Hand, Together	23

Characterization and Application of a Stationary Phase Promoter Library from Serratia marcescens	23
Repurposing Peptide Nanomaterials as Synthetic Biomolecular Condensates in Bacteria	23
Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes	22
Synthetic Genomics: Repurposing Biological Systems for Applications in Engineering Biology	22
Let There Be Light: Genome Reduction Enables Bacillus subtilis to Produce Disulfide-Bonded Gaussia Luciferase	22
Modular Synthetic Biology Toolkit for Filamentous Fungi	22
Direct Transfer and Consolidation of Synthetic Yeast Chromosomes by Abortive Mating and Chromosome Elimination	22
Nucleic Transformer: Classifying DNA Sequences with Self-Attention and Convolutions	22
Construction and Applications of Mammalian Cell-Based DNA-Encoded Peptide/Protein Libraries	22
Engineered Biosynthesis and Anticancer Studies of Ring-Expanded Antimycin-Type Depsipeptides	22
Comprehensive Parameter Space Mapping of Cell Cycle Dynamics under Network Perturbations	22
High-Yielding Terpene-Based Biofuel Production in Rhodobacter capsulatus	22
Issue Editorial Masthead	22
Correction to “Enabling Biological Nitrogen Fixation for Cereal Crops in Fertilized Fields”	21
Issue Editorial Masthead	21
Correction to “Reprogramming the Biosynthesis of Precursor Peptide to Create a Selenazole-Containing Nosiheptide Analogue”	21
Issue Publication Information	21
Issue Publication Information	21
A System for the Evolution of Protein–Protein Interaction Inducers	20
“Multiagent” Screening Improves Directed Enzyme Evolution by Identifying Epistatic Mutations	20
Pyrococcus furiosus Argonaute Based Detection Assays for Porcine Deltacoronavirus	20
Catalytically Active Inclusion Bodies─Benchmarking and Application in Flow Chemistry	20
Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens	20
Construction of a Compact Array of Microplasma Jet Devices and Its Application for Random Mutagenesis of Rhodosporidium toruloides	20
Dynamic Control Strategy to Produce Riboflavin with Lignocellulose Hydrolysate in the Thermophile Geobacillus thermoglucosidasius	20
Using High-Throughput Experiments To Screen N-Glycosyltransferases with Altered Specificities	20
Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae	20
Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit	20
Using Protein Design and Directed Evolution to Monomerize a Bright Near-Infrared Fluorescent Protein	20
Combinatorial Protein Engineering and Metabolic Engineering for Efficient Synthesis of l-Histidine in Corynebacterium glutamicum	20
Engineered l-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments	19
CRISPR-Cas12a-Based Detection of SARS-CoV-2 Harboring the E484K Mutation	19
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering	19
Predicting and Interpreting Protein Developability Via Transfer of Convolutional Sequence Representation	19
Discovery of 8-Hydroxyquinoline as a Histamine Receptor 2 Blocker Scaffold	19
Experimental Data Connector (XDC): Integrating the Capture of Experimental Data and Metadata Using Standard Formats and Digital Repositories	19
Antibiotic-Efficient Genetic Cassette for the TEM-1 β-Lactamase That Improves Plasmid Performance	19
Progress in Gene Editing and Metabolic Regulation of Saccharomyces cerevisiae with CRISPR/Cas9 Tools	19
In Vitro Transcription/Translation-Coupled DNA Replication through Partial Regeneration of 20 Aminoacyl-tRNA Synthetases	19
Modeling Microbial Communities: Perspective and Challenges	19
Degron-Controlled Protein Degradation in Escherichia coli: New Approaches and Parameters	19
Biotechnological Production of 2′-Fucosyllactose: A Prevalent Fucosylated Human Milk Oligosaccharide	19
Development of an Efficient Recombinant Protein Expression System in Clostridium saccharoperbutylacetonicum Based on the Bacteriophage T7 System	19
In Vivo Genome Editing in Type I and II Methanotrophs Using a CRISPR/Cas9 System	19
Toward Minimal Transcription–Translation Machinery	19
Investigating and Engineering an 1,2-Propanediol-Responsive Transcription Factor-Based Biosensor	19
Balancing the AspC and AspA Pathways of Escherichia coli by Systematic Metabolic Engineering Strategy for High-Efficient l-Homoserine Production	19
From De Novo to Xeno: Advancing Macromolecule Design beyond Proteins	19
Establishing CRISPRi for Programmable Gene Repression and Genome Evolution in Cupriavidus necator	19
Programmable Acetylation Modification of Bacterial Proteins by a Cas12a-Guided Acetyltransferase	19
SynHEAL: Synthesis of Health Equity, Advancement, and Leadership	19
Engineered Mutants of a Marine Photosynthetic Purple Nonsulfur Bacterium with Increased Volumetric Productivity of Polyhydroxyalkanoate Bioplastics	19
Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F₄₂₀-Dependent Bioconversions in Yeast	18
Scaling the Functional Nanopore (FuN) Screen: Systematic Evaluation of Self-Assembling Membrane Peptides and Extension with a K⁺-Responsive Fluorescent Protein Sensor	18
Control Intracellular Protein Condensates with Light	18
Bacteria Engineered to Produce Serotonin Modulate Host Intestinal Physiology	18
Stem Loop Mediated Transgene Modulation in Human T Cells	18
Issue Publication Information	18
Large Flux Supply of NAD(H) under Aerobic Conditions by Candida glycerinogenes	18
Determination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between Zymomonas mobilis and Escherichia coli	18
Regulatory Components for Bacterial Cell-Free Systems Engineering	18
Building an RNA-Based Toggle Switch Using Inhibitory RNA Aptamers	18
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield	18
CRISPR-Cas13 System as a Promising and Versatile Tool for Cancer Diagnosis, Therapy, and Research	18
Using Methyl Bromide for Interspecies Cell–Cell Signaling and As a Reporter in a Model Soil Consortium	18
Issue Publication Information	18
De Novo Biosynthesis of a Polyene-Type Ginsenoside Precursor Dammaradienol in Saccharomyces cerevisiae	18
Engineering Tunable, Low Latency Spatial Computation with Dual Input Quorum Sensing Promoters	18
Nano-Regulation of Gene Expression in Chlamydomonas reinhardtii: Harnessing AuNPs for Remotely Switchable Lipid Biosynthesis via Antisense Oligonucleotides	18
Engineering a Silk Protein-Mediated Customizable Compartment for Modular Metabolic Synthesis	18
Modulating Liposome Surface Charge for Maximized ATP Regeneration in Synthetic Nanovesicles	18
Efficient Mutasynthesis of “Non-Natural” Antitubercular Ilamycins with Low Cytotoxicity	18
Graph Computation Using Algorithmic Self-Assembly of DNA Molecules	18
Saccharomyces cerevisiae Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins	18
MIRA/PfAgo-Mediated Biosensor for Multiplex Human Enteroviruses Virus Typing Detection on HFMD	18
Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica	18
IWBDA 2022: Toward a Modular Future of Synthetic Biology Driven by Bio-Design Automation	18
An Optimized Version of the Small Synthetic Genome (Mini-Synplastome) for Plastid Metabolic Engineering in Solanum tuberosum (Potato)	18
In VivoSampling of Intracellular Heterogeneity ofPseudomonas putidaEnables Multiobjective Optimization of Genetic Devices	18
Formation of Giant Lipid Vesicle Containing Dual Functions Facilitates Outer Membrane Phospholipase	18

Heterologous Single-Strand DNA-Annealing and Binding Protein Enhance CRISPR-Based Genome Editing Efficiency in Komagataella phaffii	17
Actomyosin-Driven Division of a Synthetic Cell	17
Engineering Agrobacterium tumefaciens Adhesion to Target Cells	17
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles	17
Signal Amplification for Cell-Free Biosensors, an Analog-to-Digital Converter	17
Escherichia coli Data-Driven Strain Design Using Aggregated Adaptive Laboratory Evolution Mutational Data	17
Understanding Autologous Spliceostatin Transcriptional Regulation to Derive Parts for Heterologous Expression in a Burkholderia Bacterial Host	17
Adaptive Optimization Boosted the Production of Moenomycin A in the Microbial Chassis Streptomyces albus J1074	17
Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies	17
Engineered Attenuated Salmonella typhimurium Expressing Neoantigen Has Anticancer Effects	17
Pipette Show: An Open Source Web Application to Support Pipetting into Microplates	17
Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies	17
ACtivE: Assembly and CRISPR-Targeted in Vivo Editing for Yeast Genome Engineering Using Minimum Reagents and Time	17
An Autocatalytic Peptide Cyclase Improves Fidelity and Yield of Circular Peptides In Vivo and In Vitro	16
EngineeredSaccharomyces cerevisiaeas a Biosynthetic Platform of Nucleotide Sugars	16
Glycodiversifying Testosterone with a Promiscuous Glycosyltransferase OsSGT2 from Ornithogalum saundersiae	16
Establishment of Green- and Red-Fluorescent Reporter Proteins Based on the Fluorescence-Activating and Absorption-Shifting Tag for Use in Acetogenic and Solventogenic Anaerobes	16
Impact of Chemical Dynamics of Commercial PURE Systems on Malachite Green Aptamer Fluorescence	16
Improved Membrane Permeability via Hypervesiculation for In Situ Recovery of Lycopene in Escherichia coli	16
Reconfiguring the Escherichia coli Electron Transport Chain to Enhance trans-2-Decenoic Acid Production	16
Morphological and Metabolic Engineering of Yarrowia lipolytica to Increase β-Carotene Production	16
Interrogating the Function of Bicistronic Translational Control Elements to Improve Consistency of Gene Expression	16
Critical Assessment of Protein Engineering (CAPE): A Student Challenge on the Cloud	16
Energy Aware Technology Mapping of Genetic Logic Circuits	16
Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing	16
An Automated Cell-Free Workflow for Transcription Factor Engineering	16
Production and Characterization of Motile and Chemotactic Bacterial Minicells	16
Engineering Metabolic Pathways for Cofactor Self-Sufficiency and Serotonin Production in Escherichia coli	16
Design of a Genetically Programmable and Customizable Protein Scaffolding System for the Hierarchical Assembly of Robust, Functional Macroscale Materials	16
Metabolic Engineering of Corynebacterium glutamicum for the Production of the Four-Carbon Platform Chemicals γ-Hydroxybutyrate and γ-Butyrolactone	16
Correction to “Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit”	16
A Massively Parallel In Vivo Assay of TdT Mutants Yields Variants with Altered Nucleotide Insertion Biases	16
Kinetics of RNA and RNA:DNA Hybrid Strand Displacement	16
Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA Downregulation with Minimal Collateral RNA Cutting	16
Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds	15
Integrating CRISPR-Cas12a with a crRNA-Mediated Catalytic Network for the Development of a Modular and Sensitive Aptasensor	15
Creating RNA Specific C-to-U Editase from APOBEC3A by Separation of Its Activities on DNA and RNA Substrates	15
Discovering Content through Text Mining for a Synthetic Biology Knowledge System	15
Systems Analysis of Highly Multiplexed CRISPR-Base Editing in Streptomycetes	15
Increased Cordycepin Production in Yarrowia lipolytica Using Combinatorial Metabolic Engineering Strategies	15
Efficient Strategy for Synthesizing Vector-Free and Oncolytic Herpes Simplex Type 1 Viruses	15
Optimization of Heterologous Glucoraphanin Production In Planta	15
Characterization of Eight Bacterial Biosensors for Microbial Diagnostic and Therapeutic Applications	15
Programmable Synthetic Upstream Activating Sequence Library for Fine-Tuning Gene Expression Levels in Saccharomyces cerevisiae	15
Engineering Yarrowia lipolytica to Produce Tailored Chain-Length Fatty Acids and Their Derivatives	15
A Cell-Free Biosensor for Assessment of Hyperhomocysteinemia	15
Increasing MinD’s Membrane Affinity Yields Standing Wave Oscillations and Functional Gradients on Flat Membranes	15
Metabolic Engineering of Saccharomyces cerevisiae for Ethyl Acetate Biosynthesis	15
Design–Build–Test of Synthetic Promoters for Inducible Gene Regulation in Alphaproteobacteria	15
Bioengineering Self-Organizing Signaling Centers to Control Embryoid Body Pattern Elaboration	15
Cell-Free Biosensing Genetic Circuit Coupled with Ribozyme Cleavage Reaction for Rapid and Sensitive Detection of Small Molecules	15
Programming Dynamic Division of Labor Using Horizontal Gene Transfer	15
Engineered Bacillus subtilis as Oral Probiotics To Enhance Clearance of Blood Lactate	14
LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning	14
DIVERSIFY: A Fungal Multispecies Gene Expression Platform	14
Automated Rational Strain Construction Based on High-Throughput Conjugation	14
Rapid Visual CRISPR Assay: A Naked-Eye Colorimetric Detection Method for Nucleic Acids Based on CRISPR/Cas12a and a Convolutional Neural Network	14
A Novel Membrane-Associated Protein Aids Bacterial Colonization of Maize	14
Label-Free Detection of Transgenic Crops Using an Isothermal Amplification Reporting CRISPR/Cas12 Assay	14
A Logically Reversible Double Feynman Gate with Molecular Engineered Bacteria Arranged in an Artificial Neural Network-Type Architecture	14
Prebiotics-Controlled Disposable Engineered Bacteria for Intestinal Diseases	14
CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces	14
Correction to “Novel RNA Viral Vectors for Chemically Regulated Gene Expression in Embryonic Stem Cells”	14
The Ever-Expanding Boundaries of Synthetic Biology	14
Self-Sufficient In Vitro Multi-Enzyme Cascade for Efficient Synthesis of Danshensu froml-DOPA	14
Restructuring a Complex Genetic Function on Episomal Vectors in Escherichia coli	14
Streamlined Human Cell-Based Recombinase-Mediated Cassette Exchange Platform Enables Multigene Expression for the Production of Therapeutic Proteins	14
Deep Mutational Scanning of an Oxygen-Independent Fluorescent Protein CreiLOV for Comprehensive Profiling of Mutational and Epistatic Effects	14
Simultaneous Improvement of Limonene Production and Tolerance in Yarrowia lipolytica through Tolerance Engineering and Evolutionary Engineering	14
Codon-Restrained Method for Both Eliminating and Creating Intragenic Bacterial Promoters	14
Engineering Alternate Ligand Recognition in the PurR Topology: A System of Novel Caffeine Biosensing Transcriptional Antirepressors	14
Scaling-up Engineering Biology for Enhanced Environmental Solutions	14
Synthetic Gene Circuits Combining CRISPR Interference and CRISPR Activation in E. coli: Importance of Equal Guide RNA Binding Affinities to Avoid Context-Dependent Effects	14
Modular Pathway Compartmentalization for Agroclavine Overproduction in Saccharomyces cerevisiae	14
Parameterized Computational Framework for the Description and Design of Genetic Circuits of Morphogenesis Based on Contact-Dependent Signaling and Changes in Cell–Cell Adhesion	14
Evolution of Organic Solvent-Resistant DNA Polymerases	14
Considerations for Domestication of Novel Strains of Filamentous Fungi	14
Conversion of Mevalonate to Isoprenol Using Light Energy inEscherichia coliwithout Consuming Sugars for ATP Supply	14
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis	14
Perspectives on Genetically Engineered Microorganisms and Their Regulation in the United States	14
Systematic In Vivo Characterization of Fluorescent Protein Maturation in Budding Yeast	14
Metabolic Engineering of Aspartic Acid Supply Modules for Enhanced Production of Bacitracin in Bacillus licheniformis	14
Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning	14
Two-Layered Microfluidic Devices for High-Throughput Dynamic Analysis of Synthetic Gene Circuits in E. coli	14
Construction of an Engineered Escherichia coli with Efficient Chemotactic and Metabolizing Ability toward Tetrathionate	14
Development of a Cell-Based Biosensor for Residual Detergent Detection in Decellularized Scaffolds	14
In Vitro Transcription–Translation in an Artificial Biomolecular Condensate	14
Developing the Limosilactobacillus reuteri Chassis through an Endogenous Programmable Endonuclease-Based Genome Editing Tool	14
Development of Solid-State Storage for Cell-Free Expression Systems	14
Sequence Preference and Initiator Promiscuity for De Novo DNA Synthesis by Terminal Deoxynucleotidyl Transferase	13
Issue Editorial Masthead	13
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae	13
Systems-Level Modeling for CRISPR-Based Metabolic Engineering	13
Four-Step Pathway from Phenylpyruvate to Benzylamine, an Intermediate to the High-Energy Propellant CL-20	13
Induced CD45 Proximity Potentiates Natural Killer Cell Receptor Antagonism	13