ACS Synthetic Biology

Article	Citations
An Autocatalytic Peptide Cyclase Improves Fidelity and Yield of Circular Peptides In Vivo and In Vitro	77
Predicting and Interpreting Protein Developability Via Transfer of Convolutional Sequence Representation	74
Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds	74
LevSeq: Rapid Generation of Sequence-Function Data for Directed Evolution and Machine Learning	73
LOICA: Integrating Models with Data for Genetic Network Design Automation	71
Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens	64
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles	60
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis	59
Stem Loop Mediated Transgene Modulation in Human T Cells	59
Energy Aware Technology Mapping of Genetic Logic Circuits	59
Discovering Content through Text Mining for a Synthetic Biology Knowledge System	55
Bacteria Engineered to Produce Serotonin Modulate Host Intestinal Physiology	54
Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis	53
Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight	53
Mobile Translation Systems Generate Genomically Engineered Escherichia coli Cells with Improved Growth Phenotypes	50
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cIantisense	50
Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit	50
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids	49
Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing	49
Nano-Regulation of Gene Expression in Chlamydomonas reinhardtii: Harnessing AuNPs for Remotely Switchable Lipid Biosynthesis via Antisense Oligonucleotides	49
Engineered Mutants of a Marine Photosynthetic Purple Nonsulfur Bacterium with Increased Volumetric Productivity of Polyhydroxyalkanoate Bioplastics	47
Synthetic Biology 2.0: The Dawn of a New Era	46
Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes	46
IWBDA 2022: Toward a Modular Future of Synthetic Biology Driven by Bio-Design Automation	46
Hybrid Methylotrophic Pathway in Serratia marcescens for Sustainable Terpenoid Biosynthesis	45

A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage	45
Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida	45
A Logically Reversible Double Feynman Gate with Molecular Engineered Bacteria Arranged in an Artificial Neural Network-Type Architecture	44
Engineered l-Lactate Responding Promoter System Operating in Glucose-Rich and Anoxic Environments	43
Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many	42
Parameterized Computational Framework for the Description and Design of Genetic Circuits of Morphogenesis Based on Contact-Dependent Signaling and Changes in Cell–Cell Adhesion	41
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield	41
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	39
Efficient Mutasynthesis of “Non-Natural” Antitubercular Ilamycins with Low Cytotoxicity	39
Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis	39
Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit	38
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering	38
Protease-Responsive Toolkit for Conditional Targeted Protein Degradation	38
Combining Chemical Catalysis with Enzymatic Steps for the Synthesis of the Artemisinin Precursor Dihydroartemisinic Acid	38
In VivoSampling of Intracellular Heterogeneity ofPseudomonas putidaEnables Multiobjective Optimization of Genetic Devices	37
Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies	37
Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose	37
Construction of an Engineered Escherichia coli with Efficient Chemotactic and Metabolizing Ability toward Tetrathionate	36
Actomyosin-Driven Division of a Synthetic Cell	36
Cell-Free Protein Synthesis as a Method to Rapidly Screen Machine Learning-Generated Protease Variants	35
Development of a Type I-E CRISPR-Based Programmable Repression System for Fine-Tuning Metabolic Flux toward D-Pantothenic Acid in Bacillus subtilis	34
Combinatorial Nonribosomal Peptide Synthetase Libraries Using the SEAM-Combi-OGAB Method	34
Engineering a Synthetic Dopamine-Responsive Riboswitch for In Vitro Biosensing	33
Issue Editorial Masthead	33
Characterization of Integrase and Excisionase Activity in a Cell-Free Protein Expression System Using a Modeling and Analysis Pipeline	33
Issue Editorial Masthead	33
Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles	32
Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins in Saccharomyces cerevisiae	32
Switchable Zinc(II)-Responsive Globular β-Sheet Peptide	32
Zymo-Parts: A Golden Gate Modular Cloning Toolbox for Heterologous Gene Expression in Zymomonas mobilis	32
Systematic Engineering of Escherichia coli for Efficient Production of Nicotinamide Mononucleotide From Nicotinamide	31
High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis in Yarrowia lipolytica	31
Cell-Free Transcription-Coupled CRISPR/Cas12a Assay for Prototyping Cyanobacterial Promoters	31
Cheating the Cheater: Suppressing False-Positive Enrichment during Biosensor-Guided Biocatalyst Engineering	31
ReScribe: An Unrestrained Tool Combining Multiplex Recombineering and Minimal-PAM ScCas9 for Genome Recoding Pseudomonas putida	31
Reducing Cellular Autolysis of Bacillus subtilis to Improve Keratinase Production	31
Spatiotemporal Gene Expression by a Genetic Circuit for Chemical Production in Escherichia coli	31
GPCR-FEX: A Fluoride-Based Selection System for Rapid GPCR Screening and Engineering	30
Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles	30
Genetic Engineering of Bacteriophage K1F with Human Epidermal Growth Factor to Enhance Killing of Intracellular E. coli K1	29
Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation	29
DNA Input Classification by a Riboregulator-Based Cell-Free Perceptron	28
RNA Polymerase-Mediated Stepwise RNA-Primed RNA Polymerization for Site-Specific Multiple Labeling into RNA: A Fluorescence Resonance Energy Transfer Probe Detects the Structural Change of an RNA G-Qu	28
De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii	28
Directing Uphill Strand Displacement with an Engineered Superhelicase	28
RNA Aptamer-Mediated Gene Activation Systems for Inducible Transgene Expression in Animal Cells	28
CRISPR-Cas-Mediated Tethering Recruits the Yeast HMR Mating-Type Locus to the Nuclear Periphery but Fails to Silence Gene Expression	28
CRISPR Diagnostics for Quantification and Rapid Diagnosis of Myotonic Dystrophy Type 1 Repeat Expansion Disorders	28
An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts	28
Combating Infectious Diseases with Synthetic Biology	27

An Arrayed CRISPR Screen Identifies Knockout Combinations Improving Antibody Productivity in HEK293 Cells	27
Four-Step Pathway from Phenylpyruvate to Benzylamine, an Intermediate to the High-Energy Propellant CL-20	27
Emergent Bistable Switches from the Incoherent Feed-Forward Signaling of a Positive Feedback Loop	26
Hybrid Cas12a Variants with Relaxed PAM Requirements Expand Genome Editing Compatibility	26
RoboMoClo: A Robotics-Assisted Modular Cloning Framework for Multiple Gene Assembly in Biofoundry	26
AutoBioTech─A Versatile Biofoundry for Automated Strain Engineering	26
Engineering Yeast Yarrowia lipolytica for Methanol Assimilation	25
Combinatorial PCR Method for Efficient, Selective Oligo Retrieval from Complex Oligo Pools	25
Reconstruction of a Genome-Scale Metabolic Model of Scenedesmus obliquus and Its Application for Lipid Production under Three Trophic Modes	25
Fine-Regulating the Carbon Flux of l-Isoleucine Producing Corynebacterium glutamicum WM001 for Efficient l-Threonine Production	25
Modular Metabolic Engineering and Synthetic Coculture Strategies for the Production of Aromatic Compounds in Yeast	25
Issue Publication Information	25
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae	25
Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Cell-Free Transcription–Translation Platform	25
Functional and Structural Roles of the Dimer Interface in the Activity and Stability of Clostridium butyricum 1,3-Propanediol Oxidoreductase	25
Implementation of a Novel Optogenetic Tool in Mammalian Cells Based on a Split T7 RNA Polymerase	25
ATP-Binding Cassette Exporter PDR11-Mediated Terpenoid Secretion in Engineered Yeast	24
Special Issue on Artificial Intelligence for Synthetic Biology	24
Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform	24
One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions	24
DNA Origami Nanostructure Detection and Yield Estimation Using Deep Learning	24
Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris	24
Highly Parallelized Construction of DNA from Low-Cost Oligonucleotide Mixtures Using Data-Optimized Assembly Design and Golden Gate	24
Enabling Biological Nitrogen Fixation for Cereal Crops in Fertilized Fields	24
RNA-Based Sensor Systems for Affordable Diagnostics in the Age of Pandemics	24
Light-Induced Nanobody-Mediated Targeted Protein Degradation for Metabolic Flux Control	24
Harnessing Escherichia coli’s Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency	23
Modular and Flexible Molecular Device for Simultaneous Cytosine and Adenine Base Editing at Random Genomic Loci in Filamentous Fungi	23
Issue Publication Information	23
Engineering Escherichia coli for Perillyl Alcohol Production with Reduced Endogenous Dehydrogenation	23
Engineering a Synthetic Escherichia coli Coculture for Compartmentalized de novo Biosynthesis of Isobutyl Butyrate from Mixed Sugars	23
Issue Publication Information	23
Issue Publication Information	23
Endotoxin-Free Outer Membrane Vesicles for Safe and Modular Anticancer Immunotherapy	23
In Vivo Assembly of a Genetically Encoded Artificial Metalloenzyme for Hydrogen Production	23
Optically Controlled CRISPR-Cas9 and Cre Recombinase for Spatiotemporal Gene Editing: A Review	23
Discovery of Latent Cannabichromene Cyclase Activity in Marine Bacterial Flavoenzymes	22
Functional Modification of Cyanobacterial Phycobiliprotein and Phycobilisomes through Bilin Metabolism Control	22
Pseudotyping Bacteriophage P2 Tail Fibers to Extend the Host Range for Biomedical Applications	22
DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein Engineering	22
Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection Marker tetAOPT	22
Metabolic Engineering of Escherichia coli for Self-Induced Production of l-Isoleucine	22
Precision Transcriptome Editing	22
High-Throughput Screening of an Octanoic Acid Producer Strain Library Enables Detection of New Targets for Increasing Titers in Saccharomyces cerevisiae	22
Precision Genome Engineering in Streptococcus suis Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology	22
Hyperstable Synthetic Mini-Proteins as Effective Ligand Scaffolds	22
pUdOs: Concise Plasmids for Bacterial and Mammalian Cells	22
Iron-Tannin Coating Reduces Clearance and Increases Tumor Colonization of Systemically Delivered Bacteria	22
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts	22
A High-Throughput Colocalization Pipeline for Quantification of Mitochondrial Targeting across Different Protein Types	22
Exploration of the Native Sucrose Operon Enables the Development of an Inducible T7 Expression System in Paenibacillus polymyxa	22
Advancement of Research Progress on Synthesis Mechanism of Cannabidiol (CBD)	22
Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation	22
A Tunable and Expandable Transactivation System in Probiotic Yeast Saccharomyces boulardii	22
Amplification-Free COVID-19 Detection by Digital Droplet REVEALR	22
Marine Biofilm Engineered to Produce Current in Response to Small Molecules	21
PASIV: A Pooled Approach-Based Workflow to Overcome Toxicity-Induced Design of Experiments Failures and Inefficiencies	21
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast	21
HUHgle: An Interactive Substrate Design Tool for Covalent Protein-ssDNA Labeling Using HUH-Tags	21
Versatile Design of Intracellular Protein-Responsive Translational Regulation System for Synthetic mRNA	21
Issue Editorial Masthead	21
Synthetic Biology of Natural Products Engineering: Recent Advances Across the Discover–Design–Build–Test–Learn Cycle	21
Advances in Field Detection Based on CRISPR/Cas System	21
Production of Caffeic Acid with Co-fermentation of Xylose and Glucose by Multi-modular Engineering in Candida glycerinogenes	21
Engineered Stop and Go T7 RNA Polymerases	21
Genome-Wide CRISPR/Cas9 Screening Unveils a Novel Target ATF7IP–SETDB1 Complex for Enhancing Difficult-to-Express Protein Production	21
Issue Editorial Masthead	21
Biosynthesis of Antimicrobial Ornithine-Containing Lacticin 481 Analogues by Use of a Combinatorial Biosynthetic Pathway in Escherichia coli	21
Expanding the Cell-Free Reporter Protein Toolbox by Employing a Split mNeonGreen System to Reduce Protein Synthesis Workload	21
A Simple and Highly Sensitive Naked-Eye Analysis of EGFR 19del via CRISPR/Cas12a Triggered No-Nonspecific Nucleic Acid Amplification	21
Functional Deimmunization of Botulinum Neurotoxin Protease Domain via Computationally Driven Library Design and Ultrahigh-Throughput Screening	21
Issue Editorial Masthead	21
Synthetic Homoserine Lactone Sensors for Gram-Positive Bacillus subtilis Using LuxR-Type Regulators	20
Point-of-Care Peptide Hormone Production Enabled by Cell-Free Protein Synthesis	20
Spatially Selective Electrochemical Cleavage of a Polymerase-Nucleotide Conjugate	20
A Ubiquitous Chromatin Opening Element and DNA Demethylation Facilitate Doxycycline-Controlled Expression during Differentiation and in Transgenic Mice	20
Correction to “Toward a Circular Bioeconomy: Designing Microbes and Polymers for Biodegradation”	20
High-Efficiency Protection of Linear DNA in Cell-Free Extracts from Escherichia coli and Vibrio natriegens	20
Recombinant Protein Expression Chassis Library of Vibrio natriegens by Fine-Tuning the Expression of T7 RNA Polymerase	20
Tailoring Bacterial Cellulose through the CRISPR/Cas9-Mediated Gene Editing Tool in Komagataeibacter xylinus	20

Designing Single-Component Optogenetic Membrane Recruitment Systems: The Rho-Family GTPase Signaling Toolbox	20
Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems	20
An In Vitro Hybrid Biocatalytic System Enabled by a Combination of Surface-Displayed, Purified, and Cell-Free Expressed Enzymes	20
Highly Reversible Tunable Thermal-Repressible Split-T7 RNA Polymerases (Thermal-T7RNAPs) for Dynamic Gene Regulation	20
Design of Four Small-Molecule-Inducible Systems in the Yeast Chromosome, Applied to Optimize Terpene Biosynthesis	20
Pooled CRISPR Interference Screening Identifies Crucial Transcription Factors in Gas-Fermenting Clostridium ljungdahlii	19
Rational Engineering of Escherichia coli Nissle 1917 as Live Biotherapeutic to Degrade Uremic Toxin Precursors	19
Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of l-Pipecolic Acid from Glucose	19
Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation	19
Engineering a Synthetic Pathway for Tyrosol Synthesis in Escherichia coli	19
Reprogramming Escherichia coli Metabolism for Bioplastics Synthesis from Waste Cooking Oil	19
Channeling Anabolic Side Products toward the Production of Nonessential Metabolites: Stable Malate Production in Synechocystis sp. PCC6803	19
Metabolic Engineering of Candida glycerinogenes for Sustainable Production of Geraniol	19
Imaging Method Using CRISPR/dCas9 and Engineered gRNA Scaffolds Can Perturb Replication Timing at the HSPA1 Locus	19
Evaluating Apoptotic Gene Efficiency for CHO Culture Performance Using Targeted Integration	19
Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803	19
Conditional Control of Universal CAR T Cells by Cleavable OFF-Switch Adaptors	19
A Tail Fiber Engineering Platform for Improved Bacterial Transduction-Based Diagnostic Reagents	19
A Simple and Effective Strategy for the Development of Robust Promoter-Centric Gene Expression Tools	19
Comparison of Kill Switch Toxins in Plant-Beneficial Pseudomonas fluorescens Reveals Drivers of Lethality, Stability, and Escape	19
A Layered Genetic Design Enables the Yeast Galactose Regulon to Respond to Cyanamide	19
Low Resource Integrated Platform for Production and Analysis of Capped mRNA	19
Construction of Canthaxanthin-Producing Yeast by Combining Spatiotemporal Regulation and Pleiotropic Drug Resistance Engineering	19
Synthetic Ecosystems: From the Test Tube to the Biosphere	18
Sense-and-Respond Payload Delivery Using a Novel Antigen-Inducible Promoter Improves Suboptimal CAR-T Activation	18
Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans	18
GOLDBAR: A Framework for Combinatorial Biological Design	18
Engineering a Controllable Targeted Protein Degradation System and a Derived OR-GATE-Type Inducible Gene Expression System in Synechococcus elongatus PCC 7942	18
Synthetic Platforms for Characterizing and Targeting of SARS-CoV-2 Genome Capping Enzymes	18
Statistical Analysis and Tokenization of Epitopes to Construct Artificial Neoepitope Libraries	18
GroovDB: A Database of Ligand-Inducible Transcription Factors	18
Transfer RNA Synthesis-Coupled Translation and DNA Replication in a Reconstituted Transcription/Translation System	18
Assessment of Colorimetric Reporter Enzymes in the PURE System	18
Introduction to Engineering Biology: A Conceptual Framework for Teaching Synthetic Biology	18
Engineering Yarrowia lipolytica to Produce l-Malic Acid from Glycerol	18
Microbial Synthesis of Nucleosides: Advances and Prospects	18
Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching	18
Optical Control of Phosphoinositide Binding: Rapid Activation of Subcellular Protein Translocation and Cell Signaling	18
Organellar Glue: A Molecular Tool to Artificially Control Chloroplast–Chloroplast Interactions	18
Correction to “Hyperaccurate Ribosomes for Improved Genetic Code Reprogramming”	18
Cold Exposure and Oral Delivery of GLP-1R Agonists by an Engineered Probiotic Yeast Strain Have Antiobesity Effects in Mice	17
Efficient Autoinducible Expression of Recombinant Proteins via the DegSU Quorum Sensing System in a Robust Bacillus subtilis	17
Simulations Reveal High Efficiency and Confinement of a Population Suppression CRISPR Toxin-Antidote Gene Drive	17
A Single-Component Optogenetic Gal4-UAS System Allows Stringent Control of Gene Expression in Zebrafish and Drosophila	17
Molecular Mechanism of the Cytosine CRISPR Base Editing Process and the Roles of Translesion DNA Polymerases	17
Exploring the Tunability and Dynamic Properties of MarR-PmarO Sensor System in Escherichia coli	17
Evolutionary Stability Optimizer (ESO): A Novel Approach to Identify and Avoid Mutational Hotspots in DNA Sequences While Maintaining High Expression Levels	17
Logic Circuits Based on 2A Peptide Sequences in the Yeast Saccharomyces cerevisiae	17
One-Pot Biocatalytic Route from Alkanes to α,ω-Diamines by Whole-Cell Consortia of Engineered Yarrowia lipolytica and Escherichia coli	17
Engineering a Yeast Cell Factory to Sustainably Biosynthesize Parthenolide	17
Investigating the Specificity of the Dehydration and Cyclization Reactions in Engineered Lanthipeptides by Synechococcal SyncM	17
Conditionally Activated Cross-Linked crRNAs for CRISPR/Cas12a Based Nucleic Acid Detection	17
Robust Inducible Gene Expression in Intracellular Listeria monocytogenes In Vivo	17
Yeast Synthetic Minimal Biosensors for Evaluating Protein Production	17
Issue Editorial Masthead	17
Antibiotics-Free Steady Bioproduction of Valuable Chemicals from Organic Wastes by Engineered Vibrio natriegens through Targeted Gene Integration	17
Engineering a CRISPRi Circuit for Autonomous Control of Metabolic Flux in Escherichia coli	17
The Potential of Artificial Cells Functioning under In Situ Deep-Sea Conditions	17
Reliable Genomic Integration Sites in Pseudomonas putida Identified by Two-Dimensional Transcriptome Analysis	17
Nisin- and Ripcin-Derived Hybrid Lanthipeptides Display Selective Antimicrobial Activity against Staphylococcus aureus	17
Genetic Code Expansion in Pseudomonas putida KT2440	17
Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium	17
Engineering the Maize Root Microbiome: A Rapid MoClo Toolkit and Identification of Potential Bacterial Chassis for Studying Plant–Microbe Interactions	17
Issue Publication Information	17
Synthetic Cellobiose-Inducible Regulatory Systems Allow Tight and Dynamic Controls of Gene Expression in Streptomyces	17
Issue Publication Information	16
Bacterial Living Therapeutics with Engineered Protein Secretion Circuits to Eliminate Breast Cancer Cells	16
Efficient Production of 2′-Fucosyllactose from l-Fucose via Self-Assembling Multienzyme Complexes in Engineered Escherichia coli	16
Autoinduction AND Gate Inhibits Cell Lysis to Enhance Protein Production in Bacillus subtilis Controlled by Population Density and Cell Physiological State	16
Combining Fusion of Cells with CRISPR-Cas9 Editing for the Cloning of Large DNA Fragments or Complete Bacterial Genomes in Yeast	16
Regulating Expression of Mistranslating tRNAs by Readthrough RNA Polymerase II Transcription	16
Engineering the Entrance of a Flavonoid Glycosyltransferase Promotes the Glycosylation of Etoposide Aglycone	16
Imaging Flow Cytometry for High-Throughput Phenotyping of Synthetic Cells	16
Synthesis, Reverse Transcription, Replication, and Inter-Transcription of 2′-Modified Nucleic Acids with Evolved Thermophilic Polymerases: Efforts toward Multidimensional Expansion of the Central Dogm	16
Issue Editorial Masthead	16
The Neurospora crassa Inducible Q System Enables Simultaneous Optogenetic Amplification and Inversion in Saccharomyces cerevisiae for Bidirectional Control of Gene Expression	16
High Titer of (S)-Equol Synthesis from Daidzein in Escherichia coli	16
Sequence-Based Searching for SynBioHub Using VSEARCH	16
Enveloped Viral Replica Equipped with Spike Protein Derived from SARS-CoV-2	16
PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in Saccharomyces cerevisiae	16
Directed Evolution of Herbicide Biosensors in a Fluorescence-Activated Cell-Sorting-Compatible Yeast Two-Hybrid Platform	16
Red Light Responsive Cre Recombinase for Bacterial Optogenetics	16
Characterization and Engineering of a Novel Miniature Eubacterium siraeum CRISPR-Cas12f System	16
Identifying Conditions for Protein Synthesis Inside Giant Vesicles Using Microfluidics toward Standardized Artificial Cell Production	16
Combinatorial Engineering of Escherichia coli for Enhancing 3-Fucosyllactose Production	16
The iGEM Grand Jamboree and the Serious Fun of Engineering Biology	16
Establishing an Artificial Pathway for the Biosynthesis of Octopamine and Synephrine	16
Metabolic Perturbations to an Escherichia coli-based Cell-Free System Reveal a Trade-off between Transcription and Translation	16
Optimization of Campesterol-Producing Yeast Strains as a Feasible Platform for the Functional Reconstitution of Plant Membrane-Bound Enzymes	16
CRISPR-GEM: A Novel Machine Learning Model for CRISPR Genetic Target Discovery and Evaluation	16
Characterizing a Propionate Sensor in E. coli Nissle 1917	16
Issue Editorial Masthead	15
From De Novo to Xeno: Advancing Macromolecule Design beyond Proteins	15
Issue Publication Information	15
Issue Publication Information	15