OOIR: Observatory of International Research

Papers

(The H4-Index of ACS Synthetic Biology is 37. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-06-01 to 2026-06-01.)

Article	Citations
Synthetic Biology 2.0: The Dawn of a New Era	132
Preventing Production Escape Using an Engineered Glucose-Inducible Genetic Circuit	128
The Japan-UK Synthetic Biology Conference, Spring 2025: Strengthening Global Links to Engineer Biology	91
Highly Accurate Sequence- and Position-Independent Error Profiling of DNA Synthesis and Sequencing	87
Issue Publication Information	80
Optimal LentiCRISPR-Based System for Sequential CRISPR/Cas9 Screens	77
Stem Loop Mediated Transgene Modulation in Human T Cells	67
Strategies for Improved Docosahexaenoic Acid Synthesis in Microalgae	60
Nano-Regulation of Gene Expression in Chlamydomonas reinhardtii: Harnessing AuNPs for Remotely Switchable Lipid Biosynthesis via Antisense Oligonucleotides	59
Construction of an Engineered Escherichia coli with Efficient Chemotactic and Metabolizing Ability toward Tetrathionate	59
Hybrid Methylotrophic Pathway in Serratia marcescens for Sustainable Terpenoid Biosynthesis	57
In VivoSampling of Intracellular Heterogeneity ofPseudomonas putidaEnables Multiobjective Optimization of Genetic Devices	56
A Photoresponsive Homing Endonuclease for Programmed DNA Cleavage	54
Cell-Free Transcription and Translation Tether Expressed Peptides to Their Encoding Circular DNA	53
Issue Publication Information	53
Engineering Cyanobacterial Cell Factories for Photosynthetic Production of Fructose	51
Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many	51
Lipid Modification and Membrane Localization of Proteins in Cell-Free System	50
An Autocatalytic Peptide Cyclase Improves Fidelity and Yield of Circular Peptides In Vivo and In Vitro	45
Targeting Transcriptional and Translational Hindrances in a Modular T7RNAP Expression System in Engineered Pseudomonas putida	44
Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids	44
Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds	44
Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes	43
Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit	42
DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles	42

Actomyosin-Driven Division of a Synthetic Cell	42
Control of λ Lysogenic Escherichia coli Cells by Synthetic λ Phage Carrying cI^antisense	42
Predicting and Interpreting Protein Developability Via Transfer of Convolutional Sequence Representation	42
Maximizing Heterologous Expression of Engineered Type I Polyketide Synthases: Investigating Codon Optimization Strategies	42
Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis	42
Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield	40
Protease-Responsive Toolkit for Conditional Targeted Protein Degradation	39
Combining Chemical Catalysis with Enzymatic Steps for the Synthesis of the Artemisinin Precursor Dihydroartemisinic Acid	39
Efficient CRISPR/Cas12a-Based Genome-Editing Toolbox for Metabolic Engineering in Methanococcus maripaludis	39
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis	39
Energy Aware Technology Mapping of Genetic Logic Circuits	38
Development of a Recombineering System for the Acetogen Eubacterium limosum with Cas9 Counterselection for Markerless Genome Engineering	37
Synthetic Biology Design as a Paradigm Shift toward Manufacturing Affordable Adeno-Associated Virus Gene Therapies	37
Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight	37
Discovering Content through Text Mining for a Synthetic Biology Knowledge System	37
Directed Evolution of a SelB Variant that Does Not Require a Selenocysteine Insertion Sequence Element for Function	37
Preclinical Evaluation of Synthetic Biology-Driven Engineered Escherichia coli Nissle 1917 as a Living Therapeutic for Sustained L-DOPA Delivery	37
Metabolic Engineering of Microbial Chassis Cells for d-Pantothenate Production: A Review	37
Cell-Free Protein Synthesis as a Method to Rapidly Screen Machine Learning-Generated Protease Variants	37