Nature Biomedical Engineering

Article	Citations
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	681
Brain–heart–eye axis revealed by multi-organ imaging genetics and proteomics	483
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	429
Emergency delivery of particulate drugs by active ejection using in vivo wireless devices	372
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	360
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	300
A data-efficient strategy for building high-performing medical foundation models	298
A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence	283
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	275
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	273
Sensitive detection of SARS-CoV-2 on paper	258
Neuroimaging endophenotypes reveal underlying mechanisms and genetic factors contributing to progression and development of four brain disorders	255
Systemic immune responses to irradiated tumours via the transport of antigens to the tumour periphery by injected flagellate bacteria	255
A strain-programmed patch for the healing of diabetic wounds	249
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	244
FAS-less allogeneic CAR T cells	231
Streamlining point-of-care assays	226
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	221
Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis	216
Autonomous ultrasound with UltraBot	216
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	210
Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia	204
Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	201
A generative model uses healthy and diseased image pairs for pixel-level chest X-ray pathology localization	199
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	196

Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	196
A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide	193
Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection	191
Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity	188
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	173
Systematic functional screening of switchable aptamer beacon probes	169
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	166
Faster detection of somatic structural variants	162
Fast and sensitive electromechanical sensing	161
A warm introduction	152
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	152
Offbeat drug-delivery tactics	150
Engineered non-expansive matrix for blood vessel organoid development and ischaemic stroke repair	147
Making fitter cells and tissues	145
Simultaneous visualization of multiple radionuclides in vivo	144
Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver	138
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	135
Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker	135
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	130
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	130
Publisher Correction: Large-scale generation of iNK and CAR-iNK cells from CD34+ haematopoietic stem and progenitor cells for adoptive immunotherapy	126
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	120
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	117
Targeting inflammation with chimeric antigen receptor macrophages using a signal switch	117
Probing when dCas9 tolerates DNA mismatches	114
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	113
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates	112
Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis	112
A two-phase point-of-care diagnostic device for bladder cancer	108
A knockout run for CRISPRed cells	108
Sensing haemodynamics via wearables in sync	106
Subcutaneous pancreatic islet transplantation without immunosuppression	103
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	102
Making MRI increasingly useful	102
Nanobody-based bispecific antibody engagers targeting CTLA-4 or PD-L1 for cancer immunotherapy	100
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	99
Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes	97
Reprogramming of fibroblasts into expandable cardiovascular progenitor cells via small molecules in xeno-free conditions	97
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	96
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	95
Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	94
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	92
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	91
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution	90
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	89
Mutation corrections in spinal muscular atrophy	89
Deep screening of antibody–antigen affinities	84
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	83
Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	81
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	79

Eyeing severe diabetes upfront	78
Engineered thoracic spinal cord organoids for transplantation after spinal cord injury	78
Accelerating primer design for amplicon sequencing using large language model-powered agents	78
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	75
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	75
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	75
CAR T cells expressing a bacterial virulence factor trigger potent bystander antitumour responses in solid cancers	75
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	74
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	74
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	74
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	73
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	71
Opportunities and challenges in the development of exoskeletons for locomotor assistance	70
Straightforward, inexpensive and sensitive	70
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	70
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	70
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	70
Author Correction: Thermally sentient bionic limbs	68
Insulin independence from stem-cell-derived islets	68
Mineralized bacteria as an immunotherapy agent against various cancer types	67
Explaining counterfactual images	66
Targeting myeloid cell-mediated fibrosis through FAK	66
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	66
Engineering multi-specific nano-antibodies for cancer immunotherapy	65
Addendum: A tissue-engineered scale model of the heart ventricle	65
The ever-changing communication of scientific discovery	65
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	65
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	64
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	64
Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances	63
Multi-antigen spherical nucleic acid cancer vaccines	63
Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy	63
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	63
Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cells	63
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	63
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	63
Osteopontin attenuates the foreign-body response to silicone implants	62
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	61
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	61
Tooling up against mechanobiological orthodoxy	60
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	60
A window into solid stresses within tumours	60
Biomimetic model to study penile dysfunctions	59
One-day rapid sterility test for human-derived biopharmaceuticals	58
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens	57
Tumour-specific bioorthogonal synthesis of proteolysis-targeting chimeras and nanoparticles boosts T cell activity	57
Label-free navigation system for grading prostate tumour malignancy in situ via tissue pH and prostate-specific antigen activity	57
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	55
Efficient production of non-immunogenic, long circular RNAs for high protein yield	54
Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples	54
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	53
Antigen-bearing outer membrane vesicles as tumour vaccines produced in situ by ingested genetically engineered bacteria	53
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	53
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	52
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	52
Efficient circular RNA synthesis for potent rolling circle translation	52
Towards handy neural prostheses	51
How to make cover letters instructive	49
Seeing ageing through retinal images	49
Author Correction: Targeted tumour theranostics in mice via carbon quantum dots structurally mimicking large amino acids	49
DNA-based computation for multiple biomarkers	48
Publisher Correction: Non-invasive characterization of pericyte dysfunction in mouse brain using functional ultrasound localization microscopy	48
Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism	48
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	47
Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction	47
Continuous insulin monitoring using an antibody-protecting zwitterionic microneedle patch	46
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	46
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	46
Designing stable humanized antibodies	45
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	45
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	45
A generalist foundation model and database for open-world medical image segmentation	45
Author Correction: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity	44
A low-power stretchable neuromorphic nerve with proprioceptive feedback	44
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	44
Overcoming immune hurdles to implant longevity	43
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	42
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	42
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	41
Contextual learning is nearly all you need	40

Stimulating localizable sensations	39
Artificial intelligence advances skull stripping across lifespan	39
Localized butyrate restores gut homeostasis	39
Antimicrobial peptides boosted by ultrasound	38
A swallowable X-ray dosimeter	38
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	38
Assessing the toxicity of bispecific antibodies	38
Minimally invasive implantation of scalable high-density cortical microelectrode arrays for multimodal neural decoding and stimulation	38
A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases	37
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	37
Thermoreversibly assembled polymersomes for highly efficient loading, processing and delivery of protein and siRNA biologics	37
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	37
Macrophage-augmented organoids recapitulate the complex pathophysiology of viral diseases and enable development of multitarget therapeutics	37
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	37
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	35
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	35
A thermogalvanic cell dressing for smart wound monitoring and accelerated healing	35
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	34
Treatment of a severe vascular disease using a bespoke CRISPR–Cas9 base editor in mice	34
Clinical validation of a wearable ultrasound sensor of blood pressure	34
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	33
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	33
Streamlined detection of SARS-CoV-2 via Cas13	33
Dynamic monitoring of thousands of biochemical analytes using microsampling	33
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	33
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	33
Unconditional latent diffusion models memorize patient imaging data	32
Making CRISPR more deliverable	32
Accelerated sterility testing unlocks safe delivery of life-saving therapies	31
A compact, wireless system for continuous monitoring of breast milk expressed during breastfeeding	31
A vision–language pretrained transformer for versatile clinical respiratory disease applications	31
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	31
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	31
The analgesic potential of heavy water	31
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	31