Nature Biomedical Engineering

Article	Citations
Sensitive detection of SARS-CoV-2 on paper	834
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	648
Perilesional neuromodulation replaces lost sensorimotor function in persons with spinal cord injury	539
Human gastric multi-regional assembloids for functional parietal maturation and patient-specific modelling of antral foveolar hyperplasia	404
Emergency delivery of particulate drugs by active ejection using in vivo wireless devices	364
Brain–heart–eye axis revealed by multi-organ imaging genetics and proteomics	335
Single-nucleus transcriptomics of an engineered pig model reveals microglia–T cell interactions driving Huntington’s disease neurodegeneration	333
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	305
Nanosensor-integrated catheters for chemical mapping of bladder cancer biomarkers	281
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	258
RModBlock antisense oligonucleotides as a universal tool for precise and efficient inhibition of RNA modifications	251
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	248
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	241
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	237
Neuroimaging endophenotypes reveal underlying mechanisms and genetic factors contributing to progression and development of four brain disorders	229
A data-efficient strategy for building high-performing medical foundation models	208
FAS-less allogeneic CAR T cells	189
Streamlining point-of-care assays	188
Three-dimensional architecture of human bone marrow	185
Autonomous ultrasound with UltraBot	170
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	169
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	167
Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	165
From breadth to depth in clinical artificial intelligence evaluation	164
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	159

Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection	158
A generative model uses healthy and diseased image pairs for pixel-level chest X-ray pathology localization	158
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	150
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	149
Machine learning-guided design of mechanoadaptive bioglues for multitissue trauma and first-aid applications	146
Real-time single-particle imaging of functional lungs reveals mosaic-like patterns of aerosol deposition in alveoli	142
Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	140
Low-input deep learning platform for citrullinated peptide identification, autoantigen discovery and rheumatoid arthritis treatment stratification	136
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	132
Systematic functional screening of switchable aptamer beacon probes	131
A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide	131
A filamentary soft robotic probe for multimodal in utero monitoring of fetal health	127
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	127
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	126
A warm introduction	124
Offbeat drug-delivery tactics	119
Anchored random reverse primer sequencing for quantitative detection of novel gene fusions	116
Engineered non-expansive matrix for blood vessel organoid development and ischaemic stroke repair	116
Probing when dCas9 tolerates DNA mismatches	113
LDL-binding IL-10 reduces vascular inflammation in atherosclerotic mice	110
Publisher Correction: Large-scale generation of iNK and CAR-iNK cells from CD34+ haematopoietic stem and progenitor cells for adoptive immunotherapy	110
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	104
Faster detection of somatic structural variants	104
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	104
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	104
Targeting inflammation with chimeric antigen receptor macrophages using a signal switch	101
Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis	97
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates	97
Towards generalizable AI in medicine via Generalist–Specialist Collaboration	96
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	92
Engineering challenges and translational opportunities in emerging gene delivery platforms	91
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	91
A knockout run for CRISPRed cells	90
Sensing haemodynamics via wearables in sync	90
A two-phase point-of-care diagnostic device for bladder cancer	90
Subcutaneous pancreatic islet transplantation without immunosuppression	87
A deep learning and large language hybrid workflow for omics interpretation	87
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	86
Making MRI increasingly useful	85
mRNA delivery to the endometrium	85
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	83
Breathing life into digital twins	83
Shape-conformal porous frameworks for full coverage of neural organoids and high-resolution electrophysiology	82
A three-dimensional multi-modal foundation model for optical coherence tomography	82
Author Correction: Implanted microelectrode arrays in reinnervated muscles allow separation of neural drives from transferred polyfunctional nerves	81
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	80
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	80
Nanobody-based bispecific antibody engagers targeting CTLA-4 or PD-L1 for cancer immunotherapy	80
Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes	80
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	79

Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	75
X-ray activated platinum complex induces DNA damage and enhances cancer immunotherapy through abscopal effect	74
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	74
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	73
Mutation corrections in spinal muscular atrophy	73
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	72
Deep screening of antibody–antigen affinities	72
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	71
Where the nanocarriers go	71
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	69
Straightforward, inexpensive and sensitive	69
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	69
In vivo base editing rescues liver pathophysiology and peroxisome dysfunction in a mouse model of Zellweger spectrum disorder	68
Label-free tissue NIR-II autofluorescence imaging for visualization of human liver malignancy	68
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	68
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	68
Accelerating primer design for amplicon sequencing using large language model-powered agents	68
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	67
Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	67
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	67
Engineered thoracic spinal cord organoids for transplantation after spinal cord injury	65
Symbiotic transcatheter pacemaker for lifelong energy regeneration and therapeutic function in porcine disease model	65
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	64
Opportunities and challenges in the development of exoskeletons for locomotor assistance	64
Empowering AI data scientists using a multi-agent LLM framework with self-evolving capabilities for autonomous, tool-aware biomedical data analyses	64
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	63
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	63
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	62
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	61
Insulin independence from stem-cell-derived islets	61
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	60
Mineralized bacteria as an immunotherapy agent against various cancer types	60
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	59
A geometry aware framework enhances noninvasive mapping of whole human brain dynamics	59
Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cells	58
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	58
On the horizon in biomedical engineering	57
Automated disc device for multiplexed extracellular vesicle isolation and labelling from liquid biopsies in cancer diagnostics	57
Author Correction: Thermally sentient bionic limbs	57
The ever-changing communication of scientific discovery	57
Translating black-box medical AI models into interpretable global decision logic	56
Multi-antigen spherical nucleic acid cancer vaccines	56
Osteopontin attenuates the foreign-body response to silicone implants	55
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	55
Targeting myeloid cell-mediated fibrosis through FAK	55
Explaining counterfactual images	54
Fundamental limitations of kilohertz-frequency carriers in afferent fibre recruitment with transcutaneous spinal cord stimulation	54
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	54
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	54
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	53
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	53
Engineering multi-specific nano-antibodies for cancer immunotherapy	53
Atmospheric water harvesting for humidity control in medical devices for global health	53
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	52
Learning neuroimaging models from health system-scale data	52
Tooling up against mechanobiological orthodoxy	52
Biomimetic model to study penile dysfunctions	51
A window into solid stresses within tumours	51
Efficient production of non-immunogenic, long circular RNAs for high protein yield	50
One-day rapid sterility test for human-derived biopharmaceuticals	49
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	49
AI meets high-throughput screening of LNPs	48
Efficient circular RNA synthesis for potent rolling circle translation	47
Theoretical quantitative model and clinical outcome predictions of conductive cardiac patches for electrophysiological treatments	47
Author Correction: Rewiring oncogenic signalling to precision ablation of metastatic cancer	47
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	46
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens	46
Label-free navigation system for grading prostate tumour malignancy in situ via tissue pH and prostate-specific antigen activity	46
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	46
European Union innovation needs regulatory science excellence centres	46
Tumour-specific bioorthogonal synthesis of proteolysis-targeting chimeras and nanoparticles boosts T cell activity	46
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	46
Towards handy neural prostheses	45
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	45
Seeing ageing through retinal images	45
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	45
Designing stable humanized antibodies	44
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	44
How to make cover letters instructive	44
Beyond moonshots	44

Overcoming immune hurdles to implant longevity	43
Author Correction: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity	43
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	43
Publisher Correction: Non-invasive characterization of pericyte dysfunction in mouse brain using functional ultrasound localization microscopy	43
DNA-based computation for multiple biomarkers	43
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	42
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	42
A low-power stretchable neuromorphic nerve with proprioceptive feedback	42
Neuro-symbolic artificial intelligence in medicine	42
Whole cross-sectional human ultrasound tomography	42
Outracing antibiotic resistance with deep learning	41
Continuous insulin monitoring using an antibody-protecting zwitterionic microneedle patch	41
A generalist foundation model and database for open-world medical image segmentation	41
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	41
Voxel-scale diffusion tensor phenomapping of the healthy and pressure-overloaded human heart	40
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	40
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	40
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	40
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	40
Contextual learning is nearly all you need	39
Stimulating localizable sensations	39
Artificial intelligence advances skull stripping across lifespan	39
A swallowable X-ray dosimeter	38
4D-printed adaptive hydrogel tissue expanders for ear and breast reconstruction	38
Assessing the toxicity of bispecific antibodies	38
Streamlined detection of SARS-CoV-2 via Cas13	38
Team updates at Nature Biomedical Engineering	37
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	37
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	37
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	37
Antimicrobial peptides boosted by ultrasound	37
Macrophage-augmented organoids recapitulate the complex pathophysiology of viral diseases and enable development of multitarget therapeutics	36
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	36
Engineering bispecific antibodies for brain delivery of biotherapeutics in multiple species	35
Treatment of a severe vascular disease using a bespoke CRISPR–Cas9 base editor in mice	35
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	35
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	34
Thermoreversibly assembled polymersomes for highly efficient loading, processing and delivery of protein and siRNA biologics	34
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	34
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	34
A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases	34
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	34
Localized butyrate restores gut homeostasis	33
A thermogalvanic cell dressing for smart wound monitoring and accelerated healing	33
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	33
Minimally invasive implantation of scalable high-density cortical microelectrode arrays for multimodal neural decoding and stimulation	33
Making CRISPR more deliverable	32
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	32
Immunogenic amines on lipid nanoparticles	32
Clinical validation of a wearable ultrasound sensor of blood pressure	32
Dynamic monitoring of thousands of biochemical analytes using microsampling	32
Predictive biomarkers of response to chimeric antigen receptor (CAR) T-cell therapy for pan-haematologic cancer	31
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	31
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening	30
Accelerated sterility testing unlocks safe delivery of life-saving therapies	30
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	30
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	30
A vision–language pretrained transformer for versatile clinical respiratory disease applications	30
Unconditional latent diffusion models memorize patient imaging data	30
A stealthy neural recorder for the study of behaviour in primates	30
A foundation generative model for breast ultrasound image analysis	30