Nature Biomedical Engineering

Article	Citations
Author Correction: Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	699
Brain–heart–eye axis revealed by multi-organ imaging genetics and proteomics	490
Neuroimaging endophenotypes reveal underlying mechanisms and genetic factors contributing to progression and development of four brain disorders	444
Emergency delivery of particulate drugs by active ejection using in vivo wireless devices	404
Synaptic connectivity mapping among thousands of neurons via parallelized intracellular recording with a microhole electrode array	372
An implantable hydrogel-based phononic crystal for continuous and wireless monitoring of internal tissue strains	314
Sensitive detection of SARS-CoV-2 on paper	309
A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies	291
A data-efficient strategy for building high-performing medical foundation models	287
Systemic immune responses to irradiated tumours via the transport of antigens to the tumour periphery by injected flagellate bacteria	286
A nanoparticle probe for the imaging of autophagic flux in live mice via magnetic resonance and near-infrared fluorescence	264
A strain-programmed patch for the healing of diabetic wounds	262
Rapid and stain-free quantification of viral plaque via lens-free holography and deep learning	260
Deep mutational learning for the selection of therapeutic antibodies resistant to the evolution of Omicron variants of SARS-CoV-2	257
Author Correction: Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	248
Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity	240
Large DNA deletions occur during DNA repair at 20-fold lower frequency for base editors and prime editors than for Cas9 nucleases	237
Ultrasensitive and long-lasting bioluminescence immunoassay for point-of-care viral antigen detection	228
Cas9-mediated replacement of expanded CAG repeats in a pig model of Huntington’s disease	221
FAS-less allogeneic CAR T cells	219
Streamlining point-of-care assays	217
Autonomous ultrasound with UltraBot	204
Publisher Correction: Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	204
Unbiased discovery of autoantibodies associated with severe COVID-19 via genome-scale self-assembled DNA-barcoded protein libraries	203
Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia	201

Downregulating human leucocyte antigens on mesenchymal stromal cells by epigenetically repressing a β2-microglobulin super-enhancer	201
A generative model uses healthy and diseased image pairs for pixel-level chest X-ray pathology localization	199
Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation	193
A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells	180
Linking the genotypes and phenotypes of cancer cells in heterogenous populations via real-time optical tagging and image analysis	178
Systematic functional screening of switchable aptamer beacon probes	173
A sonosensitive diphenylalanine-based broad-spectrum antimicrobial peptide	166
Faster detection of somatic structural variants	165
Offbeat drug-delivery tactics	163
A warm introduction	163
Generation of antigen-specific mature T cells from RAG1−/−RAG2−/−B2M−/− stem cells by engineering their microenvironment	158
Fast and sensitive electromechanical sensing	153
Making fitter cells and tissues	150
Engineered non-expansive matrix for blood vessel organoid development and ischaemic stroke repair	149
Publisher Correction: Large-scale generation of iNK and CAR-iNK cells from CD34+ haematopoietic stem and progenitor cells for adoptive immunotherapy	143
Probing when dCas9 tolerates DNA mismatches	139
Engineered allogeneic T cells decoupling T-cell-receptor and CD3 signalling enhance the antitumour activity of bispecific antibodies	138
A base editor for the long-term restoration of auditory function in mice with recessive profound deafness	133
Potentiating cancer immunotherapies with modular albumin-hitchhiking nanobody–STING agonist conjugates	131
A deep-learning model for quantifying circulating tumour DNA from the density distribution of DNA-fragment lengths	126
Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor	124
Simultaneous visualization of multiple radionuclides in vivo	117
Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions	117
Masking the immunotoxicity of interleukin-12 by fusing it with a domain of its receptor via a tumour-protease-cleavable linker	116
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts	114
Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis	114
Targeting inflammation with chimeric antigen receptor macrophages using a signal switch	113
Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver	112
A knockout run for CRISPRed cells	110
A two-phase point-of-care diagnostic device for bladder cancer	109
Sensing haemodynamics via wearables in sync	106
Making MRI increasingly useful	105
Subcutaneous pancreatic islet transplantation without immunosuppression	105
An off-the-shelf otoacoustic-emission probe for hearing screening via a smartphone	104
Reprogramming of fibroblasts into expandable cardiovascular progenitor cells via small molecules in xeno-free conditions	104
Nanobody-based bispecific antibody engagers targeting CTLA-4 or PD-L1 for cancer immunotherapy	103
Mapping light distribution in tissue by using MRI-detectable photosensitive liposomes	102
A bilingual speech neuroprosthesis driven by cortical articulatory representations shared between languages	99
Multiplex imaging of amyloid-β plaques dynamics in living brains with quinoline-malononitrile-based probes	98
1H magnetic resonance spectroscopic imaging of deuterated glucose and of neurotransmitter metabolism at 7 T in the human brain	93
Targeting vaccines to dendritic cells by mimicking the processing and presentation of antigens in xenotransplant rejection	93
Challenges and opportunities of acquiring cortical recordings for chronic adaptive deep brain stimulation	92
A hydrogel-based mechanical metamaterial for the interferometric profiling of extracellular vesicles in patient samples	92
A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution	86
Mapping tissue heterogeneity in solid tumours using PET–MRI and machine learning	85
Mutation corrections in spinal muscular atrophy	82
Deep screening of antibody–antigen affinities	82
Author Correction: Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTENmRNA	81
Eyeing severe diabetes upfront	80
Engineered thoracic spinal cord organoids for transplantation after spinal cord injury	79

Accelerating primer design for amplicon sequencing using large language model-powered agents	79
Publisher Correction: Efficient in vivo base editing via single adenoassociated viruses with size-optimized genomes encoding compact adenine base editors	78
Straightforward, inexpensive and sensitive	77
Profiling protein–protein interactions to predict the efficacy of B-cell-lymphoma-2-homology-3 mimetics for acute myeloid leukaemia	76
CAR T cells expressing a bacterial virulence factor trigger potent bystander antitumour responses in solid cancers	76
Synthetic cationic helical polypeptides for the stimulation of antitumour innate immune pathways in antigen-presenting cells	76
Enhancement of the viability of T cells electroporated with DNA via osmotic dampening of the DNA-sensing cGAS–STING pathway	75
Ablation of FAS confers allogeneic CD3– CAR T cells with resistance to rejection by T cells and natural killer cells	72
Isolation of tumour-reactive lymphocytes from peripheral blood via microfluidic immunomagnetic cell sorting	72
A serine-conjugated butyrate prodrug with high oral bioavailability suppresses autoimmune arthritis and neuroinflammation in mice	72
Allometrically scaling tissue forces drive pathological foreign-body responses to implants via Rac2-activated myeloid cells	72
A battery-less wireless implant for the continuous monitoring of vascular pressure, flow rate and temperature	71
Targeted desialylation and cytolysis of tumour cells by fusing a sialidase to a bispecific T-cell engager	71
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes	70
Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity	70
An injectable subcutaneous colon-specific immune niche for the treatment of ulcerative colitis	70
Opportunities and challenges in the development of exoskeletons for locomotor assistance	70
Insulin independence from stem-cell-derived islets	69
Author Correction: Thermally sentient bionic limbs	68
Explaining counterfactual images	67
Mineralized bacteria as an immunotherapy agent against various cancer types	67
Targeting myeloid cell-mediated fibrosis through FAK	66
Concurrently depleting regulatory T cells and activating cytotoxic T cells in tumours	66
Synthetic libraries of immune cells displaying a diverse repertoire of chimaeric antigen receptors as a potent cancer immunotherapy	66
Addendum: A tissue-engineered scale model of the heart ventricle	66
The ever-changing communication of scientific discovery	66
Directed evolution-based discovery of ligands for in vivo restimulation of chimeric antigen receptor T cells	65
Generation of T-cell-receptor-negative CD8αβ-positive CAR T cells from T-cell-derived induced pluripotent stem cells	65
Osteopontin attenuates the foreign-body response to silicone implants	65
Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing	65
Author Correction: Enhancing the immunogenicity of lipid-nanoparticle mRNA vaccines by adjuvanting the ionizable lipid and the mRNA	65
Engineering multi-specific nano-antibodies for cancer immunotherapy	64
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples	64
Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances	64
Antibiotic-mediated selection of randomly mutagenized and cytokine-expressing oncolytic viruses	63
Rational immunosilencing of a promiscuous T-cell epitope in the capsid of an adeno-associated virus	63
Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells	62
Multi-antigen spherical nucleic acid cancer vaccines	61
Tooling up against mechanobiological orthodoxy	60
Base editing ablates all protein-coding genes of the mitochondrial genome in mouse cells	60
Publisher Correction: Targeting overexpressed antigens in glioblastoma via CAR T cells with computationally designed high-affinity protein binders	60
A window into solid stresses within tumours	59
Tumour-specific bioorthogonal synthesis of proteolysis-targeting chimeras and nanoparticles boosts T cell activity	59
Precise virulence inactivation using a CRISPR-associated transposase for combating Enterobacteriaceae gut pathogens	59
Biomimetic model to study penile dysfunctions	58
Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood–brain barrier	58
One-day rapid sterility test for human-derived biopharmaceuticals	56
Efficient circular RNA synthesis for potent rolling circle translation	55
Label-free navigation system for grading prostate tumour malignancy in situ via tissue pH and prostate-specific antigen activity	55
Antitumour vaccination via the targeted proteolysis of antigens isolated from tumour lysates	55
Efficient production of non-immunogenic, long circular RNAs for high protein yield	55
Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples	55
Generation of neural organoids for spinal-cord regeneration via the direct reprogramming of human astrocytes	52
Derivation of prognostic contextual histopathological features from whole-slide images of tumours via graph deep learning	52
Towards handy neural prostheses	51
Neutrophils bearing adhesive polymer micropatches as a drug-free cancer immunotherapy	51
Antigen-bearing outer membrane vesicles as tumour vaccines produced in situ by ingested genetically engineered bacteria	51
Seeing ageing through retinal images	50
How to make cover letters instructive	50
Overcoming immune hurdles to implant longevity	49
Publisher Correction: Non-invasive characterization of pericyte dysfunction in mouse brain using functional ultrasound localization microscopy	49
DNA-based computation for multiple biomarkers	49
Author Correction: Targeted tumour theranostics in mice via carbon quantum dots structurally mimicking large amino acids	49
Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism	48
A generalist foundation model and database for open-world medical image segmentation	48
Control of the antitumour activity and specificity of CAR T cells via organic adapters covalently tethering the CAR to tumour cells	47
Author Correction: Mannich reaction-based combinatorial libraries identify antioxidant ionizable lipids for mRNA delivery with reduced immunogenicity	47
Designing stable humanized antibodies	46
Fluorescence lifetime of injected indocyanine green as a universal marker of solid tumours in patients	46
Continuous insulin monitoring using an antibody-protecting zwitterionic microneedle patch	45
Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction	44
Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection	44
A low-power stretchable neuromorphic nerve with proprioceptive feedback	43
Identification of druggable regulators of cell secretion via a kinome-wide screen and high-throughput immunomagnetic cell sorting	43
A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs	43
Immunization against Zika by entrapping live virus in a subcutaneous self-adjuvanting hydrogel	42
Optimization of base editors for the functional correction of SMN2 as a treatment for spinal muscular atrophy	42
Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues	41
Stimulating localizable sensations	40
A swallowable X-ray dosimeter	40

Antimicrobial peptides boosted by ultrasound	40
Assessing the toxicity of bispecific antibodies	40
Targeted knock-ins with pseudovirus for the stable expression of large transgenes	40
Artificial intelligence advances skull stripping across lifespan	40
Quantification of intratumoural heterogeneity in mice and patients via machine-learning models trained on PET–MRI data	40
Treatment of a severe vascular disease using a bespoke CRISPR–Cas9 base editor in mice	39
Hormonally mediated mechanical remodelling of human haematopoietic stem cells enhances their bone-marrow engraftment	39
Dynamical flexible inference of nonlinear latent factors and structures in neural population activity	38
Streamlined detection of SARS-CoV-2 via Cas13	38
A swallowable X-ray dosimeter for the real-time monitoring of radiotherapy	37
Thermoreversibly assembled polymersomes for highly efficient loading, processing and delivery of protein and siRNA biologics	37
Macrophage-augmented organoids recapitulate the complex pathophysiology of viral diseases and enable development of multitarget therapeutics	37
Localized butyrate restores gut homeostasis	36
Contextual learning is nearly all you need	36
Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes	36
A deep generative model for deciphering cellular dynamics and in silico drug discovery in complex diseases	35
Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair	35
Endocisternal interfaces for minimally invasive neural stimulation and recording of the brain and spinal cord	35
Minimally invasive implantation of scalable high-density cortical microelectrode arrays for multimodal neural decoding and stimulation	35
A fast all-optical 3D photoacoustic scanner for clinical vascular imaging	35
Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy	35
Label-free intraoperative histology of bone tissue via deep-learning-assisted ultraviolet photoacoustic microscopy	35
A thermogalvanic cell dressing for smart wound monitoring and accelerated healing	35
Clinical validation of a wearable ultrasound sensor of blood pressure	34
Dynamic monitoring of thousands of biochemical analytes using microsampling	33
A compact, wireless system for continuous monitoring of breast milk expressed during breastfeeding	33
The analgesic potential of heavy water	33
Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria	33
Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting	33
Accelerated sterility testing unlocks safe delivery of life-saving therapies	33
Making CRISPR more deliverable	33
Multiplexed discrimination of SARS-CoV-2 variants via plasmonic-enhanced fluorescence in a portable and automated device	33
A vision–language pretrained transformer for versatile clinical respiratory disease applications	33
Screening oral drugs for their interactions with the intestinal transportome via porcine tissue explants and machine learning	32
Unconditional latent diffusion models memorize patient imaging data	32
Immunogenic amines on lipid nanoparticles	32
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs	32
CRISPR-GPT for agentic automation of gene-editing experiments	32
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients	32
A stealthy neural recorder for the study of behaviour in primates	31
Benchmarking foundation models as feature extractors for weakly supervised computational pathology	31
A wireless patch for the monitoring of C-reactive protein in sweat	31
A wearable electrochemical biosensor for the monitoring of metabolites and nutrients	31
Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations	30
Bridging scales in brain connectivity	30
Squeezing more out from clinical imaging	30
Targeting NK2R reverses cardiometabolic dysfunction	30
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening	30
Paired CRISPR screening libraries for studying the function of the non-coding genome at scale	30
A generalist model for enhancing brain MRIs	30
Expanded toolkits for RNA circularization	29
Gene correction for sickle cell disease hits its prime	29
An artificial cilia-based array system for sound frequency decoding and resonance-responsive drug release	28
Sensing the DNA-mismatch tolerance of catalytically inactive Cas9 via barcoded DNA nanostructures in solid-state nanopores	28
A HO-1 gene knockout using a NanoCRISPR scaffold suppresses metastasis in mouse models	28
Holographic transcranial ultrasound neuromodulation enhances stimulation efficacy by cooperatively recruiting distributed brain circuits	28
CCR5-targeted allogeneic gamma–delta CD19 chimeric antigen receptor T cells for HIV-associated B cell-malignancy immunotherapy	28
Mapping light distribution in the brain via MRI	27
Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels	27
Advanced glycation end-products as mediators of the aberrant crosslinking of extracellular matrix in scarred liver tissue	27
Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins	27
Urinary bioorthogonal reporters for the monitoring of the efficacy of chemotherapy for lung cancer and of associated kidney injury	27
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine	27
Engineering of a bespoke CRISPR base editor to treat a severe vascular disease	26
‘CRISPRing’ with therapeutic cells	26
Author Correction: Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds	26
Nanoprobes for X-ray-mediated theranostics	26
Advancing breast cancer risk stratification using multimodal AI	26
Fast capturing of deep blood flow	26
Panoramic photoacoustic computed tomography with learning-based classification enhances breast lesion characterization	25
Targeting the PIEZO1 pathway boosts T cell antitumour cytotoxicity	25
AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells	25
Tackling prediction uncertainty in machine learning for healthcare	24
Suppression of cytokine release syndrome during CAR-T-cell therapy via a subcutaneously injected interleukin-6-adsorbing hydrogel	24
Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids	24
Restoration of sensory feedback from the foot and reduction of phantom limb pain via closed-loop spinal cord stimulation	24
Generation of functionally distinct T-cell populations by altering the viscoelasticity of their extracellular matrix	24
High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays	24
Optimization of therapeutic antibodies for reduced self-association and non-specific binding via interpretable machine learning	24
A deep learning system for detecting silent brain infarction and predicting stroke risk	24
Histopathological biomarkers for predicting the tumour accumulation of nanomedicines	23
A multi-organ chip with matured tissue niches linked by vascular flow	23
Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and prime editing	23
Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon	22
Setting morphogen gradients	22
Fast volumetric scanning of living tissue	22
Oncolytic mineralized bacteria as potent locally administered immunotherapeutics	22
Publisher Correction: Compression and amplification algorithms in hearing aids impair the selectivity of neural responses to speech	22
Author Correction: Enhanced control of a brain–computer interface by tetraplegic participants via neural-network-mediated feature extraction	22
Author Correction: An organoid-based screen for epigenetic inhibitors that stimulate antigen presentation and potentiate T-cell-mediated cytotoxicity	22
Diagnosis of paediatric tuberculosis by optically detecting two virulence factors on extracellular vesicles in blood samples	22
Textile-embedded cell-free biosensors	22
An instrument-free, programmable approach for nucleic acid detection	22
Author Correction: A low-power stretchable neuromorphic nerve with proprioceptive feedback	21
Leveraging large language and vision models for knowledge extraction from large-scale image–text colonoscopy records	21