Biology Open

Article	Citations
First person – Daisy Pineda-Suazo	45
Divalent metal content in diet affects severity of manganese toxicity in Drosophila	37
First person – Rajeshwari B. R.	37
Insights into the zebrafish left–right organizer's centrosomes and cilia via volume electron microscopy	31
A year at the forefront of plasmodesmal biology	28
Tracking karyotype dynamics by flow cytometry reveals de novo chromosome duplications in laboratory cultures of Macrostomum lignan	27
Primary human neutrophils and monocytes migrate along endothelial cell boundaries to optimize search efficiency under static in vitro conditions	27
Assessing frailty in aged zebrafish using a quick pseudo-frailty index	27
Local differences in robustness to ocean acidification	26
Enhancer of zeste homolog 2 (Ezh2) is required in mouse Isl1-expressing progenitors for proper development of cardiac and skeletal hindlimb structures	23
Cuticular microstructure of the locust femur–tibia joint	22
Dkk1 inhibition restores mandibular growth in an achondroplasia mouse model	22
Concrete consequences: construction on prime honey bee habitat doubles foraging distances	20
Reorganization of the heterochromatin-associated gene-dense subcompartment in early neuronal development	20
Unpredictable aggressive defence of the venomous snake, Crotalus ravus, towards predators and humans	20
EyeHex toolbox for complete segmentation of ommatidia in fruit fly eyes	18
Hip stabilization in an australopithecine-like hip: the influence of shape on muscle activation	18
First person – Anna Lennon	17
Macrophages allocate before apoptosis initiation and produce reactive oxygen species during interdigital phagocytosis	17
Knee position affects medial gastrocnemius and soleus activation during dynamic plantarflexion: no evidence for an inter-muscle compensation in healthy young adults	16
The extracellular matrix controls stem cell specification and crypt morphology in the developing and adult mouse gut	16
Chromosomal organization of multigene families and meiotic analysis in species of Loricariidae (Siluriformes) from Brazilian Amazon, with description of a new cytotype for genus Spatuloricaria	16
Isolation, validation, and long-term culture of mouse ear fibroblasts	15
Over-expressing NadA quinolinate synthase inEscherichia colienhances the bioelectrochemistry in microbial fuel cells	15
Correction: Stable atrogin-1 (Fbxo32) and MuRF1 (Trim63) gene expression is involved in the protective mechanism in soleus muscle of hibernating Daurian ground squirrels (<	14

Fluorescent protein tags for human tropomyosin isoform comparison	14
First person – Neha Jadhav Giridhar and Bita Hambrecht	14
EFEMP1 contributes to light-dependent ocular growth in zebrafish	14
Fast & Fair peer review: a bold experiment in scientific publishing	14
First person – Jessika Wise	13
Non-seed plant research in the spotlight	13
The Endosomal Sorting Complex, ESCRT, has diverse roles in blood progenitor maintenance, lineage choice and immune response	13
Retraction: Overexpression of long non-coding RNA SBF2-AS1 promotes cell progression in esophageal squamous cell carcinoma (ESCC) by repressing miR-494 to up-regulate PFN2 expression	12
Molluscivorous red knots rapidly adjust to a plant diet	12
Manipulating mitochondrial reactive oxygen species alters survival in unexpected ways in a Drosophila Cdk5 model of neurodegeneration	12
Empowering tomorrow's leaders: the impact of the 15th Network of Young Researchers in Andrology (NYRA) meeting on male reproductive health and interdisciplinary collaboration	12
First person – Franziska Hacker	12
Shaping the future of male reproductive health: fostering talent at the 14th Network of Young Researchers in Andrology meeting	12
Shifting paradigms: phosphatases from basic biology to druggable targets	11
The relationship between locomotion and hindlimb morphology in the leopard (Panthera pardus) using a geometric morphometric approach	11
First person – Anne Cathrine Hyde	11
First person – Mikaela Scheer	11
Insulated piggyBac and FRT vectors for engineering transgenic homozygous and heterozygous eHAP cells	11
Ccn2a acts downstream of cx43 to influence joint formation during zebrafish fin regeneration	11
Parental exposure to ocean acidification impacts gamete production and physiology but not offspring performance in Nematostella vectensis	10
First person – Leah Eller	10
First person – Marinus Thein	10
A protocadherin mediates oral placode morphogenesis in the tunicate Ciona	10
Effects of blood extraction and ecophysiological experiments on stress in adult males of Liolaemus attenboroughi	10
Young minds, deeper insights: a recap of the BMAS Summer School 2023, ranging from basic research to clinical implications of bone marrow adipose tissue	10
First person – Angela Fan and Yoldas Yildiz	10
First person – Avery Hinks	10
First person – Avik Banerjee	10
Winging it: hummingbirds alter flying kinematics during molt	9
From fossils to future – Jane Francis on polar exploration and changing climates	9
Fully automated point spread function analysis using PyCalibrate	9
Social interactions among ants are impacted by food availability and group size	9
The impact of exercise on protein levels in Drosophila melanogaster	9
Ethanol downregulates gastrula gene expression and cell movement, causing symptoms of foetal alcohol spectrum disorders	9
Retrograde signaling is required for Slm35-mediated negative regulation of mitophagy in yeast	9
Holding time has limited impact on constitutive innate immune function in a long-lived Antarctic seabird, the Adélie penguin: implications for field studies	9
Feeding a rich diet supplemented with the translation inhibitor cycloheximide decreases lifespan and ovary size in Drosophila	9
The Abl-interactor Abi suppresses the function of the BRAG2 GEF family member Schizo	9
Investigating the link between morphological characteristics and diet in an island population of omnivorous reptiles (Sphenodon punctatus)	8
Neural crest development and disorders: from patient to model system and back again – the NEUcrest conference	8
Meeting proceedings of the 43rd Indian Association for Cancer Research (IACR)	8
Hotter deserts and the impending challenges for the spiny-tailed Lizard in India	8
Differential effect of ubiquitous and germline depletion of Integrator complex function on C. elegans physiology	8
First person – Isabelle Toussaint-Lardé	8
Bees display limited acclimation capacity for heat tolerance	8
Metabolic shift towards oxidative phosphorylation reduces cell-density-induced cancer-stem-cell-like characteristics in prostate cancer in vitro	8
Normotensive metabolic syndrome in Transient Receptor Potential Canonical Channel type 1 Trpc1−/− mice.	8
Response to Correspondence: Hajnal et al. ‘The role of lin-12 notch in C. elegans anchor cell proliferation’	8
Elucidating the genetic architecture of migratory timing in a songbird migrant, the great reed warbler, Acrocephalus arundinaceus	8
ASCL2 is a key regulator of the proliferation–differentiation equilibrium in the esophageal epithelium	7

Intrinsic function of the peptidylarginine deiminase PADI4 is dispensable for normal haematopoiesis	7
Protein complexes from mouse and chick brain that interact with phospho-KXGS motif tau/microtubule associated protein antibody	7
The zebrafish ETS transcription factor Fli1b functions upstream of Scl/Tal1 during embryonic hematopoiesis	7
Aging disrupts spatiotemporal regulation of germline stem cells and niche integrity	7
Separating the generational effects of temperature and viscosity on the body size of a freshwater Mesocyclops copepod	7
Coilin as a regulator of NF-kB mediated inflammation in preeclampsia	7
The placental effects of trisomy for human chromosome 21 orthologs in four mouse models of Down syndrome	7
Mutational cooperativity of RUNX1::RUNX1T1 isoform 9a and oncogenic NRAS in zebrafish myeloid leukaemia	7
Na,K-ATPase activity promotes macropinocytosis in colon cancer via Wnt signaling	7
Pacsin2 is required for endocytosis in the zebrafish pronephric tubule	7
Thermal window of exercise performance of the ecosystem engineer Lanice conchilega	7
First person – Hamna Ammar	7
Zika virus-induces metabolic alterations in fetal neuronal progenitors that could influence in neurodevelopment during early pregnancy	7
Retraction: MicroRNA-96-5p promotes proliferation, invasion and EMT of oral carcinoma cells by directly targeting FOXF2	7
SlicerMorph photogrammetry: an open-source photogrammetry workflow for reconstructing 3D models	7
Glycogen synthase is required for heat shock-mediated autophagy induction in neuronal cells	7
Single-motor and multi-motor motility properties of kinesin-6 family members	7
Retraction: Overexpression of Wnt7a enhances radiosensitivity of non-small-cell lung cancer via the Wnt/JNK pathway	7
Frequency-dependent avoidance movement of glass catfish in response to sinusoidal electrical stimulation and associated spiking patterns of electroreceptors	7
Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world	7
Reliable replicative lifespan determination of yeast with a single-channel microfluidic chip	7
First person – Kaisa Pakari	7
Insights into Octopus maya cathepsins from metatranscriptome and genome: structure evolutionary relationships and functional role prediction in digestive processes	6
Innervation of the developing kidney in vivo and in vitro	6
CDX2 dose-dependently influences the gene regulatory network underlying human extraembryonic mesoderm development	6
Changes in mitochondrial distribution occur at the axon initial segment in association with neurodegeneration in Drosophila	6
A novel technique for atraumatic transurethral catheterisation of male rats	6
Temporal integration and decision-making in crocodiles	6
Developmental and tissue-specific expression of thyrotropin-releasing hormone signaling genes in zebrafish and its association with glycemic regulation	6
Maintenance of neuronal fate and transcriptional identity	6
Cerebral organoids in primary progressive multiple sclerosis reveal stem cell and oligodendrocyte differentiation defect	6
Validation of a novel handheld lactate analyzer reveals concentration-dependent bias compared with a laboratory reference device	6
Intermittent hypoxia differentially affects metabolic and oxidative stress responses in two species of cyprinid fish	6
First person – Emily Bulger	6
Linking peak intensity of mechanically stimulated bioluminescence and cell surface area in dinoflagellates	6
Actin cytoskeleton in angiogenesis	6
Effects of vessel noise on beluga (Delphinapterus leucas) call type use: ultrasonic communication as an adaptation to noisy environments?	6
Wnt and BMP signalling direct anterior–posterior differentiation in aggregates of mouse embryonic stem cells	6
Partial role of volatile organic compounds in behavioural responses of mice to bedding from cancer-affected congeners	6
Ccdc57 regulates cilia and left-right patterning in Xenopus	6
Morphlight theory inspired by raptors: musculoskeletal modeling and muscle control in Falco peregrinus wing flapping	6
Biomechanical analysis of little penguins’ underwater locomotion from the free-ranging dive data	6
A Drosophila model of mitochondrial disease phenotypic heterogeneity	6
Experimental playback of urban noise does not affect cognitive performance in captive Australian magpies	6
microRNA-guided immunity against respiratory virus infection in human and mouse lung cells	6
Alterations in lifespan and sleep:wake duration under selective monochromes of visible light in Drosophila melanogaster	6
SOX2 and NR2F1 coordinate the gene expression program of the early postnatal visual thalamus	6
Gait speed and incline modulate peak deceleration and timing of horizontal center of mass deceleration during double support	6
First person – Latasha Ludwig	6
Vestigial-dependent induction contributes to robust patterning but is not essential for wing-fate recruitment in Drosophila	6
Hormonal regulation of miRNA during mammary gland development	6
Deletion of taf1 and taf5 in zebrafish capitulate cardiac and craniofacial abnormalities associated with TAFopathies through perturbations in metabolism	6
The Company of Biologists: a century in review	6
Characterization of alternative splicing during mammalian brain development reveals the extent of isoform diversity and potential effects on protein structural changes	6
Uncoupling of nutrient sensing and cell size control by specific defects in ceramide structure	6
Increased expression of the small lysosomal gene SVIP in the Drosophila gut suppresses pathophysiological features associated with a high-fat diet	6
Cdc48 and its co-factor Ufd1 extract CENP-A from centromeric chromatin and can induce chromosome elimination in the fission yeast Schizosaccharomyces pombe	5
Zebrafish embryos hatch early in response to chemical and mechanical indicators of predation risk, resulting in underdeveloped swimming ability of hatchling larvae	5
Ethanol induces subcellular trafficking of the RNA-binding protein, hnRNP A1, in neuronal cells in vitro, but not in the peripubertal rat brain	5
Reevaluating the relationship between EGL-43 (EVI1) and LIN-12 (Notch) during C. elegans anchor cell invasion	5
Saccharomyces cerevisiae deficient in the early anaphase release of Cdc14 can traverse anaphase I without ribosomal DNA disjunction and successfully complete meiosis	5
PatternJ: an ImageJ toolset for the automated and quantitative analysis of regular spatial patterns found in sarcomeres, axons, somites, and more	5
Tbx5 overexpression in embryoid bodies increases TAK1 expression but does not enhance the differentiation of sinoatrial node cardiomyocytes	5
First person – Ricardo Villalba-Briones	5
First person – Daniel Lenthall	5
Theoretical model of impact mitigation mechanisms inherent to the North American bison skull	5
Stress-induced elemental retention in an ectothermic vertebrate	5
Alpha-T-catenin is expressed in peripheral nerves as a constituent of Schwann cell adherens junctions	5
First person – Amy Irving	5
First person – Adam Downie	5
Experimental short-term heatwaves negatively impact body weight gain and survival during larval development in Bombus terrestris L. (Hymenoptera: Apidae)	5
Cohesin protein Smc3 influences kinocilial structure and function	5
Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells	5
The lymphatic system favours survival of a unique T. brucei population	5
First person – Ming-Hsuan Wen	5
Correction: IRS-1 increases TAZ expression and promotes osteogenic differentiation in rat bone marrow mesenchymal stem cells	5
Sox8: a multifaceted transcription factor in development and disease	5
Plpp3, a novel regulator of pluripotency exit and endodermal differentiation of mouse embryonic stem cells	5
First person – Francesca Mastropasqua and Marika Oksanen	5
Transgenic force sensors and software to measure force transmission across the mammalian nuclear envelope in vivo	5

Thinking outside the mouse: emerging vertebrate models in biomedicine	5
BBQ methods: streamlined workflows for bacterial burden quantification in infected cells by confocal microscopy	5
Gene expression and DNA methylation changes in response to hypoxia in toxicant-adapted Atlantic killifish ( Fundulus heteroclitus )	5
First person – Neydiana Pina-Lopes	5
Introducing Daniel Gorelick – a new Editor-in-Chief for Biology Open	5
PIWIL2 downregulation in colon cancer promotes transposon activity and pro-tumorigenic phenotypes	4
First person – Alexandra Venuto	4
So long, and thanks for all the papers about fish (and other organisms too)	4
IFT139 regulates Hedgehog signaling and cilia structure through ciliary protein localization	4
Beyond bold versus shy: Zebrafish exploratory behavior falls into several behavioral clusters and is influenced by strain and sex	4
WntA expression and wing transcriptomics illuminate the evolution of stripe patterns in skipper butterflies	4
Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids	4
Biology Open 2023 – a year in review	4
Connexin 43 mediated collective cell migration is independent of Golgi orientation	4
Re-assessment of the subcellular localization of Bazooka/Par-3 in Drosophila: no evidence for localization to the nucleus and the neuromuscular junction	4
Meeting Review of the ‘1st Azerbaijan Neuroscience School: Introduction’	4
Conference report: current advances in embryo model research at the International Dutch Embryo Model Meeting 2025	4
Computer vision analysis to identify episodic flapping in hovering hummingbirds	4
Characterization of nitric oxide in Octopus maya nervous system and its potential role in sensory perception	4
A genome-wide CRISPR screen implicates plasma membrane asymmetry in exogenous C6-ceramide toxicity	4
The next stage in Biology Open's support for early-career researchers	4
PTP10D-mediated cell competition is not obligately required for elimination of polarity-deficient clones	4
Hepatic leukemia factor-expressing paraxial mesoderm cells contribute to the developing brain vasculature	4
Altered epithelial development of the lateral ventricle choroid plexus in Mllt11 mutants	4
Worms about town: a citizen science project discovers microsporidian parasites of nematodes through environmental sampling	4
Severe hypoxia drives loss of ST6GAL1-mediated α2,6-sialylation in the epicardial secretome impairing angiogenic activity	4
EphA1 receptor tyrosine kinase is localized to the nucleus in rhabdomyosarcoma from multiple species	4
Behavioral trait (co)variances and plasticity in response to turbidity in wild zebrafish ( Danio rerio )	4
Ecophysiology of neotropical amphibians and reptiles: lessons learned from Colombia	4
First person – Mai Nguyen	4
Instantaneous visual genotyping and facile site-specific transgenesis via CRISPR-Cas9 and phiC31 integrase	4
First person – Meg Watt	4
Analysis of the chromatin landscape and RNA polymerase II binding at SIN3-regulated genes	4
First person – Matthew Penaso-Stinson and Summer Paulson	4
Low colostrum intake results in potential accumulation of peroxisome lipid substrates in vaginal tissue of 3-week-old gilts	4
First person – Valentina Laverde	4
Flavonols do not affect aphid load in green or senescing birch leaves but coincide with a decrease in Photosystem II functionality	4
Elliptical Fourier analysis of hominoid radius shape: implications for Ardipithecus ramidus	4
A pro-angiogenic and hypoxic zebrafish model as a novel platform for anti-angiogenic drug testing	4
Oxidative stress: A sex specific cost of parental care	4
Correction: Polyphyllin II inhibits liver cancer cell proliferation, migration and invasion through downregulated cofilin activity and the AKT/NF-κB pathway	4
AXL receptor tyrosine kinase regulates Golgi organization and function via an adhesion-Arf1 signalling axis in breast and lung cancer cell lines	4
Development of a cypovirus protein microcrystal-encapsulated Bacillus thuringiensis UV-tolerant and mosquitocidal δ-endotoxin	4
A larval zebrafish model of cardiac physiological recovery following cardiac arrest and myocardial hypoxic damage	4
Development of the dictyostelid Polysphondylium violaceum does not require secreted cAMP	4
First person – Andreas Enström	4
Discontinuous, biphasic, ontogenetic shifts in the metabolic allometry of aquatic animals?	4
insideOutside: an accessible algorithm for classifying interior and exterior points, with applications in embryology	4
trithorax is an essential regulator of cardiac Hox gene expression and anterior-posterior patterning of the	4
Neurophysiological measurements of planarian brain activity: a unique model for neuroscience research	4
First person – Fatima Batool	4
Spatial structure could explain the maintenance of alternative reproductive tactics in tree cricket males	4
Metabolic FRET sensors in intact organs: Applying spectral unmixing to acquire reliable signals	4
A Year at the Forefront of Hydrostat Motion	3
Muscle forces and the demands of turning while walking	3
Gelatin-methacryloyl hydrogel stiffness influences epithelial-mesenchymal transition in MCF7 but not MDA-MB-231 breast cancer cells in 3D culture	3
Exploring stem cell frontiers: definitions, challenges, and perspectives for regenerative medicine	3
First person – Bailey Calder	3
Meeting Review of the Cryospheric Ecosystems Conference, 1-4 September 2025, Poznań, Poland	3
Alveolar epithelial paxillin in postnatal lung alveolar development	3
Temporal transcriptomic profiling of human three-dimensional neuromuscular co-cultures	3
Reflections on the EMBO Workshop: Neuroscience of Sleep 2025	3
Bioengineering of a tumour-stroma 3D-tumouroid co-culture model of hypopharyngeal cancer	3
A high-resolution bovine mitochondrial co-expression network	3
First person – Santu Saha	3
Manipulation of the nuclear envelope-associated protein SLAP during mammalian brain development affects cortical lamination and exploratory behavior	3
First person – Christoph Bruns	3
Generation and characterization of a knockout mouse of an enhancer of EBF3	3
Early-life challenge enhances cortisol regulation in zebrafish larvae	3
HepT1-derived murine models of high-risk hepatoblastoma display vascular invasion, metastasis, and circulating tumor cells	3
Dynein-2–driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex	3
Hands-on training in structural biology, a tool for sustainable development in Africa series 4	3
In vitro forestomach digestion experiments give less-biased estimates of food composition in odontocetes	3
The uncoating of EV71 in mature late endosomes requires CD-M6PR	3
P2X7 regulates ependymo-radial glial cell proliferation in adult Danio rerio following spinal cord injury	3
2024 Argentine Group for Extracellular Vesicles (GAVE) Workshop: promoting science in challenging times	3
SPS1 deficiency-triggered PGRP-LC and Toll expression controls innate immunity in Drosophila S2 cells	3
miR-302 regulates pancreatic progenitor pool and pancreatic size	3
Zebrafish model reveals developmental and hematopoietic functions of ADAMTS13	3
Ammonia transport mediated by urea transporter A isoforms	3
First person – Sophie Kraunsoe	3
Metabolic stress and muscle mechanics: Acute response of isolated soleus and EDL muscles to prolonged fasting in mice with distinct muscle phenotypes	3
Flat mount preparation for whole-mount fluorescent imaging of zebrafish embryos	3
Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure	3
Minimal functional domains of the core polarity regulator Dlg	3
Differential effects of Mendelian GDAP1 clinical variants on mitochondria-lysosome membrane contacts sites	3
foxg1a is required for hair cell development and regeneration in the zebrafish lateral line	3
Inherited anoxia tolerance and growth performance can result in enhanced invasiveness in hybrid fish	3
Allorecognition behaviors in Myxomycetes respond to intraspecies factors	3
First person – Sarit Pal	3
Constructing a doxycycline-inducible system for an epithelial-to-mesenchymal transition model in MCF10A cells	3
A simple, fast and inexpensive approach using E. coli to detect and estimate vitamin B12 content in microbial extracts	3
Computational tools for quantifying actin filament numbers, lengths, and bundling	3
First person – Martha S. C. Xelhuantzi	3
First person – Jon Bell	3