New Biotechnology

Article	Citations
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system	347
Enzymatic degradation of biopolyesters as a key strategy for bioplastic circularity	166
Production of bacterial cellulose by Komagataeibacter nataicola NRIC 0616 on various culture media	117
Plant-based production of oral vaccines for sustainable fish aquaculture	98
Microbial synthesis of cell penetrating peptides to facilitate the active principle penetration in cosmetics	93
Basic plant research in service of crop breeding – the Application Laboratory for Agricultural Research in Czech Republic	92
Identification and biosynthesis of an autoinducing cyclopeptide from Clostridium acetobutylicum that regulates solvent biosynthesis and cell morphology	84
Advancing fracture repair: gelatin-starch adhesive technique	74
Structure-function landscape models: a new avenue towards the precision engineering of microbial consortia	61
Co-liquefaction of oleaginous yeast and algal biomass: strategic enhancement of bio-crude oil productivity	50
Whole-cell bacterial biosensors for detection of structurally-diverse chemicals	48
3D printing: Economical and supply chain independent single-use plasticware for cell culture	46
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16	44
MutS-mediated rapid and cost-effective error correction in in vitro DNA synthesis	38
More than meets the eye: microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis	38
Successful combination of lactic acid bacteria and yeast fermentation and enzymatic treatment to re-cycle industrial bread by-products for bread making	35
EBRC: Enhancing bioeconomy through research and communication	33
A computational-guided framework and versatile assembly method for genome-wide CRISPRi downregulation in metabolic engineering studies	29
OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment	29
Viability assessment and biomass valorization of recombinant Komagataella phaffii	29
Tailored enzyme expression modifies Shewanella oneidensis biofilms and increases current density	29
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts	28
Optimisation of rhamnolipids production using response surface methodology	28
Balancing act: improving the environmental and economic performance of industrial-scale basic oxygen furnace gas fermentation for isopropyl alcohol production	26
Recombinant production of bovine serum albumin (BSA) in yeast: optimization and enhanced blocking capacity in immunoassays	26

Recombinant production of a killer yeast toxin in Pichia pastoris and Bacillus subtilis	26
Simultaneous saccharification and fermentation for D-lactic acid production at high temperature by a thermally-adapted homolactogenic Escherichia coli strain	26
Repurposing starchy potato washing slurries and footwear dust in the development of 3D bioplastic packaging - a circular economy approach	25
Sustainable solutions: maximizing resources using agro-industrial by-products for dairy products fortification	25
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	23
Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses	23
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein	23
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	22
Strategies for the lipidation of bioactive molecules: chemo-enzymatic synthesis of epoxidized fatty acids and their functionalization	22
Downstream process for the purification of biotechnologically produced D-DIBOA	22
Biotechnological production of valuable monomers and biopolymers from textile waste blends	22
Is human oversight to AI systems still possible?	22
Ene reductase catalysed formation of chiral carbon(sp3)-sulfur (II) centres	22
Microbial conversion of waste algal biomass to zeaxanthin by using Cellulophaga omnivescoria MSK1 strain	22
Human biological system mimic nano-complexation for pharmaceuticals/cosmetics/functional foods	21
Identification of halophilic phage genomes from a solar saltern through shotgun sequencing: a metagenomic study	21
Highly specific metal-binding peptides for selective resource recovery from spent lithium-ion batteries	20
Engineering of NAD-dependent dehydrogenases to change cofactor specificity towards NMN	19
A COST Action on microbial responses to low pH: Developing links and sharing resources across the academic-industrial divide	18
CO2 and H2-based bio-hexanol production process development with an adapted strain of Clostridium carboxidivorans	18
Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers	18
Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases	17
The evolution of reproductive mode in the Carassius auratus complex	17
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source	17
Comparative transcriptomics of CAZy enzymes in white- and brown-rot agaricomycetes: Evolutionary insights into lignocellulose degradation and the relevance of GH16 glycoside hydrolase functional diver	16
Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast	15
Nucleic acid aptamers protect against lead (Pb(II)) toxicity	15
Protein coding regions prediction by fusing DNA shape features	15
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential	15
Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation	14
Using control bias to identify initial targets for bioproduction improvement	13
Determination of the oxygen mass transfer coefficients in a bioreactor with mechanical mixing for Poly(3-Hydroxybutyrate) production	13
A Nakaseomyces glabratus CRISPRi platform to study the mode of action of novel antifungals	13
Physiology of ethanol-grown Cyberlindnera jadinii: towards sustainable protein production	13
Monitoring metagenomes using long-read nanopore sequencing	13
Discovery of a new class of bacterial heme-containing C C cleaving oxygenases	13
Biotechnological potential of amphisin produced on black cumin oilseed cake	13
Beyond iron: harnessing siderophores for the comprehensive mobilization of essential soil elements	13
Synthetic biology of acetogenic bacteria for sustainable C1 gas bioconversion	12
From the discovery of novel enzymes to their synthetic application: investigating and harnessing natural biodiversity toward efficient and selective bioprocesses	12
Enhancing sequestration of heavy metals using exopolysaccharides produced by cyanobacteria grown at ultra-high CO2 levels	12
Arsenic removal process from acid mine drainage using bacteria	12
A search for new compounds for candidosis treatment – screening of Antarctic bacteria from the IBA collection	12
Establishment of an RNA-based transient expression system in the green alga Chlamydomonas reinhardtii	11
Corrigendum to “Integration of strain and process optimization to increase autotrophic growth of engineered Komagataella phaffii” New Biotechnol 91 (2026) 110–119	11
Immobilization of His6-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes	11
Optimizing ferritin nanoparticle production for enhanced antigen presentation in vaccine development	11
Enhancing enzymatic rhamnolipid synthesis: comparative analysis of sustainable energy sources	11
Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors	11
Future food court in an urban shopping center can supply feedstocks for microbial protein production	11

Earth: Extinguishing anthropogenic risks through harmonization	11
Extraction and properties of protein extracted from fungal fermentations	11
Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production	11
Systematic identification of genomic hotspots for high-yield protein production in CHO cells	11
Biostimulation effect of different amendments on Cr(VI) recovering microbial community	11
Towards a fungal-based colorful future: upscale of colorant production from several fungal species isolated from Dutch parks	10
Utilising a genetically encoded biosensor for in-line monitoring of H2O2 generation during methanol assimilation in the yeast Komagataella phaffii	10
Development of chemically defined, DMSO-free cryopreservation media for mammalian cells	10
Valorisation of wastewater treatment sewage scum through the production of biofuels	10
Energy-efficient clostridial butanol production via histidine kinase regulation to improve strain tolerance and process robustness	10
Evaluation and optimization of CO2 capture and biomass valorization into value-added products by the green non-sulfur bacteria Chloroflexus aurantiacus	10
Intensified butanol production through optimisation of integrated membrane bioreactor operation	10
Bacterial microcompartments: the new biotechnological tool for maximizing catabolic pathway efficiency in microorganisms	10
De novo production of valuable aromatic aldehydes and aldehyde-derived products by engineered solvent-tolerant and oxidation-deficient Pseudomonas taiwanensis VLB120	10
Conversion of food processing residues into sustainable soil improvers by using enzymatic hydrolysis	10
3-hydroxypropionic acid production in engineered Cupriavidus necator H16	9
Exploring the importance of palmitoylation: alkyl chain length of lipidated proteins affects their interaction with lipid membranes and vesicular transport	9
Catalytic versatility of lipoxygenase from Microcystis aeruginosa	9
Bacterial cell immobilization using the miniaturized adhesive protein AtaA for efficient bioprocesses	9
Enzymatic recycling of polyhydroxyalkanoate (PHA) bioplastics using PHA depolymerases	9
Studying biosorption materials for water PFAS removal in a flow-through configuration	9
Canola meal protein hydrolysate as a sustainable medium for heterotrophic cultivation of Euglena gracilis	9
Upgraded activity assays for PET-degrading enzymes	9
Valorization of bioethanol by-products to produce unspecific peroxygenase with Agrocybe aegerita: Technological and proteomic perspectives	9
Valorization of prosecco wine lees for sustainable polyhydroxyalkanoates production by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034	9
Prenylation of diverse indole derivatives by the fungal aromatic prenyltransferase RePT	9
Unlocking the potential of bioproducts from waste streams for soil restoration	8
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365	8
An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics	8
XPORT ENTRAP: A droplet microfluidic platform for enhanced DNA transfer between microbial species	8
Development of integrated biofoundry for productions of fuels and chemicals from CO2 and unutilized biomass	8
Discovering novel bacterial biosynthetic potential from draft genomic data	8
In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions	8
Biodegradable zein/PEG nanofibers incorporated with natural antimicrobial compounds for eco-friendly food packaging	8
A versatile odor detection system based on automatically trained rats for chemical sensing	8
Catabolism and production of sugar acids by Corynebacterium glutamicum	8
Single-cell proteins polyhydroxyalkanoates-rich microbial biomass from municipal and winery waste as potential additive for aquafeeds	8
Primary challenges in the upcycling of genetically modified spent microbial biomass	8
Anti-biofilm molecules from Antarctic marine bacteria as weapons against microbial infections	7
Comparison of alpha-glucuronidase production by E. coli HMS174(DE3) and BL21(DE3)	7
Surface layer structure of the periodontal health-associated bacterium Tannerella serpentiformis	7
Plastics active enzymes for polyolefine and polyester degradation and derivatization	7
Significance of poly(ethylene terephthalate) (PET) substrate crystallinity on enzymatic degradation	7
A counter-selection system exploiting phenotypic heterogeneity for improved production of glutathione from yeast	7
Treatment of real oilfield produced wastewater by ionizing radiation: disinfection and changes in bacterial community and predictive function	7
Bioanalytical technologies for equitable women’s healthcare: development of ultrasensitive assays and bioinspired nanomaterials for biomarker-based diagnostics	7
Human milk oligosaccharides: high-yield production of a new food ingredient	7
Impact of standardization in tissue processing: the performance of different fixatives	7
Improved taxadiene production by optimizing DXS expression and fusing short-chain prenyltransferases	7
The pretreatment of sugar, calcium and vitamin C to enhance the color, texture and flavor properties of frozen avocado	7
Fungal G-protein coupled receptors (GPCRs): potential novel drug targets?	7
Carotenoids-based biohybrid perovskite solar cells mimicking natural photosystem.	7
Development of a bioprocess for the production of a herbicidal sugar as sustainable alternative to glyphosate	7
Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness	7
Engineering synthetic metabolons using self-assembled protein scaffolds	7
Evaluation of metallurgical dust recycling applying concepts of bioleaching	6
Green physical processes for the extraction of organic dye betacyanin from dragon fruit (Hylocereus purpusii) peels	6
Bioactive steroids from cortisone bioconversion: antioxidant properties in ocular model	6
Investigating and comparing dihydrogen thresholds and consumption kinetics of different methanogenic species	6
Modeling of a bioreactor with immobilized laccase for antibiotic removal considering the effects of diffusion-convection-reaction	6
Influence of biochar on biohydrogen production in dark fermentation	6
Multi-enzymatic approaches involving engineered D-amino acid dehydrogenases for the stereoselective synthesis of D-phenylalanines	6
Unraveling the proteomics landscape of medium composition in the model biodesulfurization strain Rhodococcus qingshengii IGTS8	6
Transgenesis in pea (Pisum sativum L.) and flax (Linum usitatissimum L.): Agritec´s experience	6
Getting a grip on oxygen transfer in bioreactors: automated kLa determination using the software Lucullus®	6
C-glycosyltransferase discovery and engineering for the synthesis of bioactive glycosides	6
N-arylated 1,4-diazabicyclo[2.2.2]octane quaternary ammonium salts as a new approach for thiol and selenol luminescent probes design	6
Exploring yeast diversity for ethanol-fueled industrial biotechnology	5
Semi-continuous cultivation of EPS-producing marine cyanobacteria: A green biotechnology to remove dissolved metals obtaining metal-organic materials	5
Functional characterization of top-down community assembly in a simple environment	5
Utilising CO2 as a feedstock for renewable chemicals: biocatalytic production of L-lactic acid from CO2 and ethanol	5
Computer-aided characterization and evaluation of enzymes active sites	5
From methanol to medicine: sustainable bioproduction of polyketides	5
Transcription factor-based biosensors for detection of naturally occurring phenolic acids	5
Engineering enhanced signal peptides: A high-throughput computational pipeline for optimizing therapeutic protein production in CHO cells	5
Lipopeptide production from xylose and its action in rheology modification for potential industrial application	5
Antibody-oligonucleotide conjugation based on cyanine dyes as cross-linking reagents	5
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	5
Metabolic regulation by the chromatin remodeler Ino80 under stress conditions and improvement of yeast stress tolerance for efficient bioproduction	5
Upcycling cotton textile waste into lactic acid by chemo-mechanical pretreatment, separate hydrolysis and fermentation with engineered Saccharomyces cerevisiae strain	5

Precision fermentation and downstream processing of fungal colorants	5
Cultivation at a liquid–liquid interface prevents phenotypic heterogeneity of human induced pluripotent stem-derived retinal pigment epithelial cells	4
Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b	4
Soft sensor for viable cell counting by measuring dynamic oxygen uptake rate	4
Engineering Pseudomonas putida for bioproduction of alkylamines	4
Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production	4
Effect of the stringent response on amylase production and the ribosome profile of Bacillus subtilis	4
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: The periodic hydrodynamic loading and the shear stress accumulation.	4
Designing a hybrid integrated bottled photobioreactor system for enhanced biomass yield and CO2 fixation during Limnospira fusiformis cultivation	4
Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation	4
The microalgal sector in Europe: Towards a sustainable bioeconomy	4
Improving the efficiency of chemical production from one-carbon substrates in Komagataella phaffii through enzyme and metabolic engineering	4
Cell-free synthesis of human norovirus capsid protein VP1	4
Isolation and characterization of novel proteins from octopus	4
Corrigendum to “More than meets the eye: Microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis”[New Biotechnol 91 (2026), 192–200]	4
Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast	4
Effect of operating conditions on the microbial selection of P(3HB-co-3HV) producers in open continuous culture	4
Pairwise residues coevolution of Candida antarctica lipase B for efficient synthesis of δ-dodecalactone	4
Exosome-like nanoparticles from Arbutus unedo L mitigate LPS-induced inflammation via JAK-STAT inactivation	4
Efficient full-length IgG secretion and sorting from single yeast clones in droplet picoreactors	4
ReViTA: A novel in vitro transcription system to study gene regulation	4
Biotechnological recovery of critical raw materials from end-of-life lithium-ion batteries	3
Enzymatic production of (S)-citronellol from citral using an Old Yellow Enzyme and a short-chain alcohol dehydrogenase	3
Bacillus thuringiensis strain with the transcriptional fusion phaR-GFP and Acetyl CoA-GFP promotor regions to assess bioplastic accumulation	3
Production of a plant-based vaccine candidate against COVID-19 through molecular pharming	3
Beyond similarity: Unveiling the distinctive transcriptional regulatory roles of ARA1 in plant biomass utilization by Myceliophthora thermophila and related fungi	3
Explainability and causability for artificial intelligence-supported medical image analysis in the context of the European In Vitro Diagnostic Regulation	3
Development of a self-powered electric and UV disinfection system based on a microbial electrochemical system	3
Gene essentiality in the solventogenic Clostridium acetobutylicum DSM 792	3
Multifunctional bioplastic coating derived from starch-rich potato washing slurries and tomato pomace for components of footwear	3
Biotechnology applied to soybean cultivation: the use of microorganisms to increase drought tolerance	3
Diversifying bioactive natural products: prenylation of plant phenolics and indole derivatives by fungal prenyltransferases	3
Production of nicotinamide mononucleotide by high cell density culture of metabolically engineered Escherichia coli	3
Targeting enzymes for plastic degradation: developing Affimers as novel plastic-binding modules	3
Upcycling plastic waste: microbial synthesis of bulk chemicals from poly(ethylene terephthalate) (PET)	3
New vectors for urea-inducible recombinant protein production	3
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen	3
Zero-shot deep learning with multi-objective optimization improves thermostability of zearalenone hydrolase and xylanase	3