New Biotechnology

Article	Citations
A multi-omics study to boost continuous bolaform sophorolipid production	284
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system	130
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16	77
EBRC: Enhancing bioeconomy through research and communication	70
3D printing: Economical and supply chain independent single-use plasticware for cell culture	70
Tailored enzyme expression modifies Shewanella oneidensis biofilms and increases current density	63
Successful combination of lactic acid bacteria and yeast fermentation and enzymatic treatment to re-cycle industrial bread by-products for bread making	58
MutS-mediated rapid and cost-effective error correction in in vitro DNA synthesis	57
Enzymatic degradation of biopolyesters as a key strategy for bioplastic circularity	44
Production of bacterial cellulose by Komagataeibacter nataicola NRIC 0616 on various culture media	41
Plant-based production of oral vaccines for sustainable fish aquaculture	38
Basic plant research in service of crop breeding – the Application Laboratory for Agricultural Research in Czech Republic	36
Microbial synthesis of cell penetrating peptides to facilitate the active principle penetration in cosmetics	36
Identification and biosynthesis of an autoinducing cyclopeptide from Clostridium acetobutylicum that regulates solvent biosynthesis and cell morphology	34
Advancing fracture repair: gelatin-starch adhesive technique	33
Structure-function landscape models: a new avenue towards the precision engineering of microbial consortia	31
Co-liquefaction of oleaginous yeast and algal biomass: strategic enhancement of bio-crude oil productivity	30
Whole-cell bacterial biosensors for detection of structurally-diverse chemicals	28
OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment	27
A computational-guided framework and versatile assembly method for genome-wide CRISPRi downregulation in metabolic engineering studies	26
Optimisation of rhamnolipids production using response surface methodology	25
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts	24
Recombinant production of bovine serum albumin (BSA) in yeast: optimization and enhanced blocking capacity in immunoassays	24
Simultaneous saccharification and fermentation for D-lactic acid production at high temperature by a thermally-adapted homolactogenic Escherichia coli strain	24
Recombinant production of a killer yeast toxin in Pichia pastoris and Bacillus subtilis	23

Sustainable solutions: maximizing resources using agro-industrial by-products for dairy products fortification	21
Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses	21
Balancing act: improving the environmental and economic performance of industrial-scale basic oxygen furnace gas fermentation for isopropyl alcohol production	21
Repurposing starchy potato washing slurries and footwear dust in the development of 3D bioplastic packaging - a circular economy approach	21
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein	21
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	20
Downstream process for the purification of biotechnologically produced D-DIBOA	20
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	20
Using control bias to identify initial targets for bioproduction improvement	20
Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress	20
Is human oversight to AI systems still possible?	20
Biotechnological production of valuable monomers and biopolymers from textile waste blends	19
Ene reductase catalysed formation of chiral carbon(sp3)-sulfur (II) centres	19
Microbial conversion of waste algal biomass to zeaxanthin by using Cellulophaga omnivescoria MSK1 strain	19
Strategies for the lipidation of bioactive molecules: chemo-enzymatic synthesis of epoxidized fatty acids and their functionalization	19
Identification of halophilic phage genomes from a solar saltern through shotgun sequencing: a metagenomic study	18
Human biological system mimic nano-complexation for pharmaceuticals/cosmetics/functional foods	18
Engineering of NAD-dependent dehydrogenases to change cofactor specificity towards NMN	17
The evolution of reproductive mode in the Carassius auratus complex	17
Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers	17
CO2 and H2-based bio-hexanol production process development with an adapted strain of Clostridium carboxidivorans	17
Highly specific metal-binding peptides for selective resource recovery from spent lithium-ion batteries	17
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source	17
Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast	17
Discovery of a new class of bacterial heme-containing C C cleaving oxygenases	17
A COST Action on microbial responses to low pH: Developing links and sharing resources across the academic-industrial divide	16
Opinion: Independent third-party entities as a model for validation of commercial antibodies	16
Protein coding regions prediction by fusing DNA shape features	16
Nucleic acid aptamers protect against lead (Pb(II)) toxicity	15
Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation	15
Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases	15
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential	15
Biotechnological potential of amphisin produced on black cumin oilseed cake	14
Determination of the oxygen mass transfer coefficients in a bioreactor with mechanical mixing for Poly(3-Hydroxybutyrate) production	14
Physiology of ethanol-grown Cyberlindnera jadinii: towards sustainable protein production	14
A Nakaseomyces glabratus CRISPRi platform to study the mode of action of novel antifungals	14
Monitoring metagenomes using long-read nanopore sequencing	14
Beyond iron: harnessing siderophores for the comprehensive mobilization of essential soil elements	13
From the discovery of novel enzymes to their synthetic application: investigating and harnessing natural biodiversity toward efficient and selective bioprocesses	13
Synthetic biology of acetogenic bacteria for sustainable C1 gas bioconversion	13
A search for new compounds for candidosis treatment – screening of Antarctic bacteria from the IBA collection	13
Enhancing sequestration of heavy metals using exopolysaccharides produced by cyanobacteria grown at ultra-high CO2 levels	13
Arsenic removal process from acid mine drainage using bacteria	13
Enhancing enzymatic rhamnolipid synthesis: comparative analysis of sustainable energy sources	12
Future food court in an urban shopping center can supply feedstocks for microbial protein production	12
Establishment of an RNA-based transient expression system in the green alga Chlamydomonas reinhardtii	12
Extraction and properties of protein extracted from fungal fermentations	12
Earth: Extinguishing anthropogenic risks through harmonization	12
Biostimulation effect of different amendments on Cr(VI) recovering microbial community	12
Systematic identification of genomic hotspots for high-yield protein production in CHO cells	12

Immobilization of His6-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes	12
Optimizing ferritin nanoparticle production for enhanced antigen presentation in vaccine development	11
A fattening factor to quantify the accumulation ability of microorganisms under N-starvation	11
Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production	11
XPORT ENTRAP: A droplet microfluidic platform for enhanced DNA transfer between microbial species	11
High-throughput screening for heterotrophic growth in microalgae using the Biolog Plate assay	11
Exploration and structure-based engineering of alkenal double bond reductases catalyzing the Cα Cβ double bond reduction of coniferaldehyde	11
Biodegradable zein/PEG nanofibers incorporated with natural antimicrobial compounds for eco-friendly food packaging	11
Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors	11
Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea	11
Bacterial microcompartments: the new biotechnological tool for maximizing catabolic pathway efficiency in microorganisms	10
Valorisation of wastewater treatment sewage scum through the production of biofuels	10
Towards a fungal-based colorful future: upscale of colorant production from several fungal species isolated from Dutch parks	10
Energy-efficient clostridial butanol production via histidine kinase regulation to improve strain tolerance and process robustness	9
De novo production of valuable aromatic aldehydes and aldehyde-derived products by engineered solvent-tolerant and oxidation-deficient Pseudomonas taiwanensis VLB120	9
Utilising a genetically encoded biosensor for in-line monitoring of H2O2 generation during methanol assimilation in the yeast Komagataella phaffii	9
Evaluation and optimization of CO2 capture and biomass valorization into value-added products by the green non-sulfur bacteria Chloroflexus aurantiacus	9
Intensified butanol production through optimisation of integrated membrane bioreactor operation	8
Enzymatic recycling of polyhydroxyalkanoate (PHA) bioplastics using PHA depolymerases	8
Valorization of bioethanol by-products to produce unspecific peroxygenase with Agrocybe aegerita: Technological and proteomic perspectives	8
Conversion of food processing residues into sustainable soil improvers by using enzymatic hydrolysis	8
Exploring the importance of palmitoylation: alkyl chain length of lipidated proteins affects their interaction with lipid membranes and vesicular transport	8
Bacterial cell immobilization using the miniaturized adhesive protein AtaA for efficient bioprocesses	8
3-hydroxypropionic acid production in engineered Cupriavidus necator H16	8
Development of chemically defined, DMSO-free cryopreservation media for mammalian cells	8
Upgraded activity assays for PET-degrading enzymes	8
In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions	8
An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics	7
Valorization of prosecco wine lees for sustainable polyhydroxyalkanoates production by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034	7
A versatile odor detection system based on automatically trained rats for chemical sensing	7
Single-cell proteins polyhydroxyalkanoates-rich microbial biomass from municipal and winery waste as potential additive for aquafeeds	7
Anti-biofilm molecules from Antarctic marine bacteria as weapons against microbial infections	7
Fungal G-protein coupled receptors (GPCRs): potential novel drug targets?	7
Catabolism and production of sugar acids by Corynebacterium glutamicum	7
A cell-free high throughput assay for assessment of SARS-CoV-2 neutralizing antibodies	7
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365	7
Unlocking the potential of bioproducts from waste streams for soil restoration	7
Carotenoids-based biohybrid perovskite solar cells mimicking natural photosystem.	7
Treatment of real oilfield produced wastewater by ionizing radiation: disinfection and changes in bacterial community and predictive function	7
Comparison of alpha-glucuronidase production by E. coli HMS174(DE3) and BL21(DE3)	7
In vivo evolution of lactic acid hyper-tolerant Clostridium thermocellum	7
Development of integrated biofoundry for productions of fuels and chemicals from CO2 and unutilized biomass	7
Discovering novel bacterial biosynthetic potential from draft genomic data	7
The pretreatment of sugar, calcium and vitamin C to enhance the color, texture and flavor properties of frozen avocado	7
Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness	7
Bioanalytical technologies for equitable women’s healthcare: development of ultrasensitive assays and bioinspired nanomaterials for biomarker-based diagnostics	7
Engineering synthetic metabolons using self-assembled protein scaffolds	6
Development of a bioprocess for the production of a herbicidal sugar as sustainable alternative to glyphosate	6
Impact of standardization in tissue processing: the performance of different fixatives	6
Modeling of a bioreactor with immobilized laccase for antibiotic removal considering the effects of diffusion-convection-reaction	6
Plastics active enzymes for polyolefine and polyester degradation and derivatization	6
Human milk oligosaccharides: high-yield production of a new food ingredient	6
Improved taxadiene production by optimizing DXS expression and fusing short-chain prenyltransferases	6
N-arylated 1,4-diazabicyclo[2.2.2]octane quaternary ammonium salts as a new approach for thiol and selenol luminescent probes design	6
Significance of poly(ethylene terephthalate) (PET) substrate crystallinity on enzymatic degradation	6
Surface layer structure of the periodontal health-associated bacterium Tannerella serpentiformis	6
A counter-selection system exploiting phenotypic heterogeneity for improved production of glutathione from yeast	6
Unraveling the proteomics landscape of medium composition in the model biodesulfurization strain Rhodococcus qingshengii IGTS8	6
Investigating and comparing dihydrogen thresholds and consumption kinetics of different methanogenic species	6
Influence of biochar on biohydrogen production in dark fermentation	5
Getting a grip on oxygen transfer in bioreactors: automated kLa determination using the software Lucullus®	5
C-glycosyltransferase discovery and engineering for the synthesis of bioactive glycosides	5
From methanol to medicine: sustainable bioproduction of polyketides	5
Computer-aided characterization and evaluation of enzymes active sites	5
Evaluation of metallurgical dust recycling applying concepts of bioleaching	5
Green physical processes for the extraction of organic dye betacyanin from dragon fruit (Hylocereus purpusii) peels	5
Antibody-oligonucleotide conjugation based on cyanine dyes as cross-linking reagents	5
Transcription factor-based biosensors for detection of naturally occurring phenolic acids	5
Transgenesis in pea (Pisum sativum L.) and flax (Linum usitatissimum L.): Agritec´s experience	5
Bioactive steroids from cortisone bioconversion: antioxidant properties in ocular model	5
Multi-enzymatic approaches involving engineered D-amino acid dehydrogenases for the stereoselective synthesis of D-phenylalanines	5
Exploring yeast diversity for ethanol-fueled industrial biotechnology	5
Bioinformatics based discovery of new keratinases in protease family M36	5
Effect of the stringent response on amylase production and the ribosome profile of Bacillus subtilis	4
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: The periodic hydrodynamic loading and the shear stress accumulation.	4
Lipopeptide production from xylose and its action in rheology modification for potential industrial application	4
Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast	4
Precision fermentation and downstream processing of fungal colorants	4
Cell-free synthesis of human norovirus capsid protein VP1	4
Isolation and characterization of novel proteins from octopus	4

Upcycling cotton textile waste into lactic acid by chemo-mechanical pretreatment, separate hydrolysis and fermentation with engineered Saccharomyces cerevisiae strain	4
Semi-continuous cultivation of EPS-producing marine cyanobacteria: A green biotechnology to remove dissolved metals obtaining metal-organic materials	4
Metabolic regulation by the chromatin remodeler Ino80 under stress conditions and improvement of yeast stress tolerance for efficient bioproduction	4
Utilising CO2 as a feedstock for renewable chemicals: biocatalytic production of L-lactic acid from CO2 and ethanol	4
Efficient full-length IgG secretion and sorting from single yeast clones in droplet picoreactors	4
ReViTA: A novel in vitro transcription system to study gene regulation	4
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	4
Functional characterization of top-down community assembly in a simple environment	4
The Jo-In protein welding system is a relevant tool to create CBM-containing plant cell wall degrading enzymes	4
Engineering Pseudomonas putida for bioproduction of alkylamines	3
The microalgal sector in Europe: Towards a sustainable bioeconomy	3
Improving the efficiency of chemical production from one-carbon substrates in Komagataella phaffii through enzyme and metabolic engineering	3
New vectors for urea-inducible recombinant protein production	3
Enzymatic production of (S)-citronellol from citral using an Old Yellow Enzyme and a short-chain alcohol dehydrogenase	3
Soft sensor for viable cell counting by measuring dynamic oxygen uptake rate	3
Biotechnological recovery of critical raw materials from end-of-life lithium-ion batteries	3
Pairwise residues coevolution of Candida antarctica lipase B for efficient synthesis of δ-dodecalactone	3
Gene essentiality in the solventogenic Clostridium acetobutylicum DSM 792	3
Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components	3
Upcycling plastic waste: microbial synthesis of bulk chemicals from poly(ethylene terephthalate) (PET)	3
Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production	3
Production of nicotinamide mononucleotide by high cell density culture of metabolically engineered Escherichia coli	3
Effect of operating conditions on the microbial selection of P(3HB-co-3HV) producers in open continuous culture	3
Multifunctional bioplastic coating derived from starch-rich potato washing slurries and tomato pomace for components of footwear	3
Exosome-like nanoparticles from Arbutus unedo L mitigate LPS-induced inflammation via JAK-STAT inactivation	3
Plant expression systems as an economical alternative for the production of iELISA coating antigen AHSV VP7	3
Diversifying bioactive natural products: prenylation of plant phenolics and indole derivatives by fungal prenyltransferases	3
Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation	3
Development of a self-powered electric and UV disinfection system based on a microbial electrochemical system	3
Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b	3
Targeting enzymes for plastic degradation: developing Affimers as novel plastic-binding modules	3
Editorial Board	2
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen	2
Conversion of syngas-derived C2 carbon sources to C2+ biochemicals by microbial cell factories	2
Unlocking the power of Moringa oleifera: a surprising synergy with cereal-based products	2
Advancements in flavonoid glycosylation: unlocking bioproduction potential with Escherichia coli W as novel platform for large-scale production	2
Bacillus thuringiensis strain with the transcriptional fusion phaR-GFP and Acetyl CoA-GFP promotor regions to assess bioplastic accumulation	2
A gradient phage-assisted continuous evolution method for screening suppressor tRNAs in Escherichia coli	2
Gramicidin S production through exclusive use of agrifood byproducts as nutritional sources	2
Antifungal activity of biosurfactants against plant pathogenic fungi	2
Domain shifts in dermoscopic skin cancer datasets: Evaluation of essential limitations for clinical translation	2
Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034	2
Immobilization of Dietzia sp. KDB1 bacteria on electrospun fiber mats for indigo reduction	2
Serotonin overproduction in Saccharomyces cerevisiae: a metabolic engineering approach	2
Fungal waste treats water waste: enzymatic and non-enzymatic removal of organic micropollutants with spent mushroom substrate of Agaricus bisporus	2
Biotechnology applied to soybean cultivation: the use of microorganisms to increase drought tolerance	2
Production of a plant-based vaccine candidate against COVID-19 through molecular pharming	2
A systems-view and optimization of bacteria with synthetic reductive glycine pathway for C1-assimilation	2
Explainability and causability for artificial intelligence-supported medical image analysis in the context of the European In Vitro Diagnostic Regulation	2
Editorial Board	2
Going beyond the limit: engineering mRNA decay and protein translation initiation enhances recombinant protein secretion	2
Shedding light on the black box of a neural network used to detect prostate cancer in whole slide images by occlusion-based explainability	2
Virtual screening of bioactive peptides with antimicrobial activity targeting carbonic anhydrase II from Escherichia coli	2
Recovery of C6 carboxylate from CO₂-based microbial electrosynthesis	2
Production and characterization of novel Anti-HIV Fc-fusion proteins in plant-based systems: Nicotiana benthamiana & tobacco BY-2 cell suspension	2
Caffeine-regulated molecular switches for functional control of CAR T cells	2
Influence of whey protein on black soybean anthocyanins’ thermostability and inhibitory effect toward xanthine oxidase	2