New Biotechnology

Article	Citations
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system	341
More than meets the eye: microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis	159
Tailored enzyme expression modifies Shewanella oneidensis biofilms and increases current density	115
Enzymatic degradation of biopolyesters as a key strategy for bioplastic circularity	95
Production of bacterial cellulose by Komagataeibacter nataicola NRIC 0616 on various culture media	93
Plant-based production of oral vaccines for sustainable fish aquaculture	85
Microbial synthesis of cell penetrating peptides to facilitate the active principle penetration in cosmetics	75
Basic plant research in service of crop breeding – the Application Laboratory for Agricultural Research in Czech Republic	72
Identification and biosynthesis of an autoinducing cyclopeptide from Clostridium acetobutylicum that regulates solvent biosynthesis and cell morphology	58
Advancing fracture repair: gelatin-starch adhesive technique	50
Structure-function landscape models: a new avenue towards the precision engineering of microbial consortia	47
Co-liquefaction of oleaginous yeast and algal biomass: strategic enhancement of bio-crude oil productivity	46
Whole-cell bacterial biosensors for detection of structurally-diverse chemicals	45
EBRC: Enhancing bioeconomy through research and communication	38
OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment	38
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16	37
3D printing: Economical and supply chain independent single-use plasticware for cell culture	34
Successful combination of lactic acid bacteria and yeast fermentation and enzymatic treatment to re-cycle industrial bread by-products for bread making	32
MutS-mediated rapid and cost-effective error correction in in vitro DNA synthesis	30
A computational-guided framework and versatile assembly method for genome-wide CRISPRi downregulation in metabolic engineering studies	29
Viability assessment and biomass valorization of recombinant Komagataella phaffii	29
Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts	28
Optimisation of rhamnolipids production using response surface methodology	28
Recombinant production of bovine serum albumin (BSA) in yeast: optimization and enhanced blocking capacity in immunoassays	27
Simultaneous saccharification and fermentation for D-lactic acid production at high temperature by a thermally-adapted homolactogenic Escherichia coli strain	27

Balancing act: improving the environmental and economic performance of industrial-scale basic oxygen furnace gas fermentation for isopropyl alcohol production	26
Recombinant production of a killer yeast toxin in Pichia pastoris and Bacillus subtilis	26
Sustainable solutions: maximizing resources using agro-industrial by-products for dairy products fortification	26
Repurposing starchy potato washing slurries and footwear dust in the development of 3D bioplastic packaging - a circular economy approach	25
Is human oversight to AI systems still possible?	24
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein	24
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	23
Microbial conversion of waste algal biomass to zeaxanthin by using Cellulophaga omnivescoria MSK1 strain	23
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	23
Downstream process for the purification of biotechnologically produced D-DIBOA	23
Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses	23
Ene reductase catalysed formation of chiral carbon(sp3)-sulfur (II) centres	22
Strategies for the lipidation of bioactive molecules: chemo-enzymatic synthesis of epoxidized fatty acids and their functionalization	22
Identification of halophilic phage genomes from a solar saltern through shotgun sequencing: a metagenomic study	22
Biotechnological production of valuable monomers and biopolymers from textile waste blends	22
Highly specific metal-binding peptides for selective resource recovery from spent lithium-ion batteries	21
Human biological system mimic nano-complexation for pharmaceuticals/cosmetics/functional foods	21
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential	20
CO2 and H2-based bio-hexanol production process development with an adapted strain of Clostridium carboxidivorans	20
Engineering of NAD-dependent dehydrogenases to change cofactor specificity towards NMN	20
Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast	19
Protein coding regions prediction by fusing DNA shape features	19
Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers	18
A COST Action on microbial responses to low pH: Developing links and sharing resources across the academic-industrial divide	18
The evolution of reproductive mode in the Carassius auratus complex	17
Using control bias to identify initial targets for bioproduction improvement	17
Comparative transcriptomics of CAZy enzymes in white- and brown-rot agaricomycetes: Evolutionary insights into lignocellulose degradation and the relevance of GH16 glycoside hydrolase functional diver	17
Nucleic acid aptamers protect against lead (Pb(II)) toxicity	16
Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation	16
Discovery of a new class of bacterial heme-containing C C cleaving oxygenases	15
Adaptive laboratory evolution of Actinobacillus succinogenes for enhanced acid tolerance and succinic acid production	15
Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases	15
Physiology of ethanol-grown Cyberlindnera jadinii: towards sustainable protein production	14
A Nakaseomyces glabratus CRISPRi platform to study the mode of action of novel antifungals	14
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source	14
Biotechnological potential of amphisin produced on black cumin oilseed cake	14
Enhancing sequestration of heavy metals using exopolysaccharides produced by cyanobacteria grown at ultra-high CO2 levels	13
Determination of the oxygen mass transfer coefficients in a bioreactor with mechanical mixing for Poly(3-Hydroxybutyrate) production	13
Arsenic removal process from acid mine drainage using bacteria	13
Monitoring metagenomes using long-read nanopore sequencing	13
From the discovery of novel enzymes to their synthetic application: investigating and harnessing natural biodiversity toward efficient and selective bioprocesses	13
Beyond iron: harnessing siderophores for the comprehensive mobilization of essential soil elements	13
Synthetic biology of acetogenic bacteria for sustainable C1 gas bioconversion	13
A search for new compounds for candidosis treatment – screening of Antarctic bacteria from the IBA collection	13
Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production	12
Enhancing enzymatic rhamnolipid synthesis: comparative analysis of sustainable energy sources	12
Extraction and properties of protein extracted from fungal fermentations	12
Establishment of an RNA-based transient expression system in the green alga Chlamydomonas reinhardtii	12
Systematic identification of genomic hotspots for high-yield protein production in CHO cells	11
Corrigendum to “Integration of strain and process optimization to increase autotrophic growth of engineered Komagataella phaffii” New Biotechnol 91 (2026) 110–119	11

Immobilization of His6-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes	11
Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors	11
Earth: Extinguishing anthropogenic risks through harmonization	11
Exploration and structure-based engineering of alkenal double bond reductases catalyzing the Cα Cβ double bond reduction of coniferaldehyde	11
Bacterial microcompartments: the new biotechnological tool for maximizing catabolic pathway efficiency in microorganisms	10
Valorisation of wastewater treatment sewage scum through the production of biofuels	10
Future food court in an urban shopping center can supply feedstocks for microbial protein production	10
Optimizing ferritin nanoparticle production for enhanced antigen presentation in vaccine development	10
Towards a fungal-based colorful future: upscale of colorant production from several fungal species isolated from Dutch parks	10
Biostimulation effect of different amendments on Cr(VI) recovering microbial community	10
XPORT ENTRAP: A droplet microfluidic platform for enhanced DNA transfer between microbial species	10
Energy-efficient clostridial butanol production via histidine kinase regulation to improve strain tolerance and process robustness	10
Prenylation of diverse indole derivatives by the fungal aromatic prenyltransferase RePT	10
De novo production of valuable aromatic aldehydes and aldehyde-derived products by engineered solvent-tolerant and oxidation-deficient Pseudomonas taiwanensis VLB120	9
Development of chemically defined, DMSO-free cryopreservation media for mammalian cells	9
Intensified butanol production through optimisation of integrated membrane bioreactor operation	9
Valorization of bioethanol by-products to produce unspecific peroxygenase with Agrocybe aegerita: Technological and proteomic perspectives	9
3-hydroxypropionic acid production in engineered Cupriavidus necator H16	9
Evaluation and optimization of CO2 capture and biomass valorization into value-added products by the green non-sulfur bacteria Chloroflexus aurantiacus	9
Exploring the importance of palmitoylation: alkyl chain length of lipidated proteins affects their interaction with lipid membranes and vesicular transport	9
Enzymatic recycling of polyhydroxyalkanoate (PHA) bioplastics using PHA depolymerases	9
Single-cell proteins polyhydroxyalkanoates-rich microbial biomass from municipal and winery waste as potential additive for aquafeeds	9
Utilising a genetically encoded biosensor for in-line monitoring of H2O2 generation during methanol assimilation in the yeast Komagataella phaffii	9
Conversion of food processing residues into sustainable soil improvers by using enzymatic hydrolysis	9
Upgraded activity assays for PET-degrading enzymes	9
Bacterial cell immobilization using the miniaturized adhesive protein AtaA for efficient bioprocesses	9
Catalytic versatility of lipoxygenase from Microcystis aeruginosa	9
Biodegradable zein/PEG nanofibers incorporated with natural antimicrobial compounds for eco-friendly food packaging	8
Development of integrated biofoundry for productions of fuels and chemicals from CO2 and unutilized biomass	8
Unlocking the potential of bioproducts from waste streams for soil restoration	8
Carotenoids-based biohybrid perovskite solar cells mimicking natural photosystem.	8
Studying biosorption materials for water PFAS removal in a flow-through configuration	8
In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions	8
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365	8
Discovering novel bacterial biosynthetic potential from draft genomic data	8
The pretreatment of sugar, calcium and vitamin C to enhance the color, texture and flavor properties of frozen avocado	8
Canola meal protein hydrolysate as a sustainable medium for heterotrophic cultivation of Euglena gracilis	8
An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics	8
A versatile odor detection system based on automatically trained rats for chemical sensing	8
Catabolism and production of sugar acids by Corynebacterium glutamicum	8
Valorization of prosecco wine lees for sustainable polyhydroxyalkanoates production by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034	8
Anti-biofilm molecules from Antarctic marine bacteria as weapons against microbial infections	7
Comparison of alpha-glucuronidase production by E. coli HMS174(DE3) and BL21(DE3)	7
Surface layer structure of the periodontal health-associated bacterium Tannerella serpentiformis	7
Plastics active enzymes for polyolefine and polyester degradation and derivatization	7
Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness	7
Treatment of real oilfield produced wastewater by ionizing radiation: disinfection and changes in bacterial community and predictive function	7
Bioanalytical technologies for equitable women’s healthcare: development of ultrasensitive assays and bioinspired nanomaterials for biomarker-based diagnostics	7
Human milk oligosaccharides: high-yield production of a new food ingredient	7
Impact of standardization in tissue processing: the performance of different fixatives	7
Engineering synthetic metabolons using self-assembled protein scaffolds	7
Fungal G-protein coupled receptors (GPCRs): potential novel drug targets?	7
A counter-selection system exploiting phenotypic heterogeneity for improved production of glutathione from yeast	7
Development of a bioprocess for the production of a herbicidal sugar as sustainable alternative to glyphosate	7
Improved taxadiene production by optimizing DXS expression and fusing short-chain prenyltransferases	7
Unraveling the proteomics landscape of medium composition in the model biodesulfurization strain Rhodococcus qingshengii IGTS8	6
Modeling of a bioreactor with immobilized laccase for antibiotic removal considering the effects of diffusion-convection-reaction	6
Bioactive steroids from cortisone bioconversion: antioxidant properties in ocular model	6
Multi-enzymatic approaches involving engineered D-amino acid dehydrogenases for the stereoselective synthesis of D-phenylalanines	6
Significance of poly(ethylene terephthalate) (PET) substrate crystallinity on enzymatic degradation	6
N-arylated 1,4-diazabicyclo[2.2.2]octane quaternary ammonium salts as a new approach for thiol and selenol luminescent probes design	6
Influence of biochar on biohydrogen production in dark fermentation	6
Getting a grip on oxygen transfer in bioreactors: automated kLa determination using the software Lucullus®	6
C-glycosyltransferase discovery and engineering for the synthesis of bioactive glycosides	6
Vitrification-Based Tissue-to-Organoid Cryopreservation Workflow for Colorectal Cancer	6
Investigating and comparing dihydrogen thresholds and consumption kinetics of different methanogenic species	6
Transgenesis in pea (Pisum sativum L.) and flax (Linum usitatissimum L.): Agritec´s experience	6
Green physical processes for the extraction of organic dye betacyanin from dragon fruit (Hylocereus purpusii) peels	6
Evaluation of metallurgical dust recycling applying concepts of bioleaching	6
Engineering enhanced signal peptides: A high-throughput computational pipeline for optimizing therapeutic protein production in CHO cells	5
From methanol to medicine: sustainable bioproduction of polyketides	5
Antibody-oligonucleotide conjugation based on cyanine dyes as cross-linking reagents	5
Precision fermentation and downstream processing of fungal colorants	5
Functional characterization of top-down community assembly in a simple environment	5
Exploring yeast diversity for ethanol-fueled industrial biotechnology	5
Computer-aided characterization and evaluation of enzymes active sites	5
Bioinformatics based discovery of new keratinases in protease family M36	5
Lipopeptide production from xylose and its action in rheology modification for potential industrial application	5
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	5
Semi-continuous cultivation of EPS-producing marine cyanobacteria: A green biotechnology to remove dissolved metals obtaining metal-organic materials	5

Transcription factor-based biosensors for detection of naturally occurring phenolic acids	5
Metabolic regulation by the chromatin remodeler Ino80 under stress conditions and improvement of yeast stress tolerance for efficient bioproduction	5
Utilising CO2 as a feedstock for renewable chemicals: biocatalytic production of L-lactic acid from CO2 and ethanol	4
ReViTA: A novel in vitro transcription system to study gene regulation	4
Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b	4
Designing a hybrid integrated bottled photobioreactor system for enhanced biomass yield and CO2 fixation during Limnospira fusiformis cultivation	4
Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast	4
Corrigendum to “More than meets the eye: Microalgal-bacterial association in polyhydroxybutyrate (PHB) accumulating cultures of Desmodesmus communis”[New Biotechnol 91 (2026), 192–200]	4
Soft sensor for viable cell counting by measuring dynamic oxygen uptake rate	4
Isolation and characterization of novel proteins from octopus	4
Cell-free synthesis of human norovirus capsid protein VP1	4
Efficient full-length IgG secretion and sorting from single yeast clones in droplet picoreactors	4
Engineering Pseudomonas putida for bioproduction of alkylamines	4
The microalgal sector in Europe: Towards a sustainable bioeconomy	4
Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation	4
Improving the efficiency of chemical production from one-carbon substrates in Komagataella phaffii through enzyme and metabolic engineering	4
Effect of operating conditions on the microbial selection of P(3HB-co-3HV) producers in open continuous culture	4
Effect of the stringent response on amylase production and the ribosome profile of Bacillus subtilis	4
Upcycling cotton textile waste into lactic acid by chemo-mechanical pretreatment, separate hydrolysis and fermentation with engineered Saccharomyces cerevisiae strain	4
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: The periodic hydrodynamic loading and the shear stress accumulation.	4
Cultivation at a liquid–liquid interface prevents phenotypic heterogeneity of human induced pluripotent stem-derived retinal pigment epithelial cells	4
Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production	4
Pairwise residues coevolution of Candida antarctica lipase B for efficient synthesis of δ-dodecalactone	4
Digitalisation and automation in smart biomanufacturing: uncertainties, challenges and early pathways towards safe and sustainable design	4
Biotechnological recovery of critical raw materials from end-of-life lithium-ion batteries	3
Enzymatic production of (S)-citronellol from citral using an Old Yellow Enzyme and a short-chain alcohol dehydrogenase	3
Upcycling plastic waste: microbial synthesis of bulk chemicals from poly(ethylene terephthalate) (PET)	3
Gene essentiality in the solventogenic Clostridium acetobutylicum DSM 792	3
Zero-shot deep learning with multi-objective optimization improves thermostability of zearalenone hydrolase and xylanase	3
Recovery of C6 carboxylate from CO₂-based microbial electrosynthesis	3
Production of a plant-based vaccine candidate against COVID-19 through molecular pharming	3
Development of a self-powered electric and UV disinfection system based on a microbial electrochemical system	3
Exosome-like nanoparticles from Arbutus unedo L mitigate LPS-induced inflammation via JAK-STAT inactivation	3
Production of nicotinamide mononucleotide by high cell density culture of metabolically engineered Escherichia coli	3
New vectors for urea-inducible recombinant protein production	3
Biotechnology applied to soybean cultivation: the use of microorganisms to increase drought tolerance	3
Unlocking the power of Moringa oleifera: a surprising synergy with cereal-based products	3
Multifunctional bioplastic coating derived from starch-rich potato washing slurries and tomato pomace for components of footwear	3
Targeting enzymes for plastic degradation: developing Affimers as novel plastic-binding modules	3
Diversifying bioactive natural products: prenylation of plant phenolics and indole derivatives by fungal prenyltransferases	3
Bacillus thuringiensis strain with the transcriptional fusion phaR-GFP and Acetyl CoA-GFP promotor regions to assess bioplastic accumulation	3
Plant expression systems as an economical alternative for the production of iELISA coating antigen AHSV VP7	3
Caffeine-regulated molecular switches for functional control of CAR T cells	3
Fungal waste treats water waste: enzymatic and non-enzymatic removal of organic micropollutants with spent mushroom substrate of Agaricus bisporus	3
Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034	2
Influence of whey protein on black soybean anthocyanins’ thermostability and inhibitory effect toward xanthine oxidase	2
Immobilization of Dietzia sp. KDB1 bacteria on electrospun fiber mats for indigo reduction	2
Advancements in flavonoid glycosylation: unlocking bioproduction potential with Escherichia coli W as novel platform for large-scale production	2
Virtual screening of bioactive peptides with antimicrobial activity targeting carbonic anhydrase II from Escherichia coli	2
Production and characterization of novel Anti-HIV Fc-fusion proteins in plant-based systems: Nicotiana benthamiana & tobacco BY-2 cell suspension	2
Shedding light on the black box of a neural network used to detect prostate cancer in whole slide images by occlusion-based explainability	2
Identification and expression of novel polyester-degrading enzymes for application on mixed plastic-containing waste	2
Explainability and causability for artificial intelligence-supported medical image analysis in the context of the European In Vitro Diagnostic Regulation	2
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen	2
Going beyond the limit: engineering mRNA decay and protein translation initiation enhances recombinant protein secretion	2
Editorial Board	2
Gramicidin S production through exclusive use of agrifood byproducts as nutritional sources	2
A gradient phage-assisted continuous evolution method for screening suppressor tRNAs in Escherichia coli	2
Tailoring structure-property relationships of fungal mycelium for material applications: A process engineering approach for pure mycelium-based biomaterials	2
Effects of zingerone nanoparticles on anti-angiogenesis in vitro and in vivo	2
Glucose to nitrogen ratio effect for phycocyanin productivity under hetero- and mixotrophic conditions with the microalga Galdieria sulphuraria	2
Domain shifts in dermoscopic skin cancer datasets: Evaluation of essential limitations for clinical translation	2
Conversion of syngas-derived C2 carbon sources to C2+ biochemicals by microbial cell factories	2
A systems-view and optimization of bacteria with synthetic reductive glycine pathway for C1-assimilation	2
Serotonin overproduction in Saccharomyces cerevisiae: a metabolic engineering approach	2
Antifungal activity of biosurfactants against plant pathogenic fungi	2
Beyond similarity: Unveiling the distinctive transcriptional regulatory roles of ARA1 in plant biomass utilization by Myceliophthora thermophila and related fungi	2
Continuous VFA production from lignocellulosic biomass via an artificial rumen reactor and membrane filtration: the cow as inspiration	2
Applying a transaminase for the sustainable production of 5-hydroxymethylfurfurylamine using cadaverine	2
Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content	1
Development of a cost-effective production of cathelicidins in barley by molecular farming	1
Introducing a heterologous ribulose monophosphate pathway to Komagataella phaffii to increase energy efficiency on methanol	1
Engineering a heterologously expressed fructosyltransferase from Aspergillus oryzae N74 in Komagataella phaffii (Pichia pastoris) for kestose production	1
Site-specifically immobilized D-amino acid dehydrogenase for the synthesis of D-phenylalanine	1
CaRA – A multi-purpose phage display library for selection of calcium-regulated affinity proteins	1
Evaluation of the respiration by changes in the agitation rates and its effect in alginate production	1
Inhibition of Fusarium oxysporum growth by lactic acid bacteria	1
Restricting bacterial bioreporter survival following field dispersal	1
Deciphering integration loci of CHO manufacturing cell lines using long read nanopore sequencing	1
Strategies for the scale-up of the bioproduction of the biodegradable herbicide D-DIBOA	1
Unlocking cell-free protein synthesis in Pichia pastoris	1
Deciphering the dynamics of auxin-induced transcriptomic and chromatin accessibility changes during barley crown root development	1
Microbial synthesis of polyhydroxyalkanoate blends with engineered Pseudomonas putida	1
Towards polyethylene terephthalate valorisation into PHB using an engineered Comamonas testosteroni strain	1
Comparative genomics and flavor characterization of five selected lactic acid bacteria provide flavor metabolic pathways predictions in fermented muskmelon puree	1
A quest for an effective and efficient compartmentalization strategy for CFD-based analyses of bioreactors	1
Cyclic peptides as a new class of plant biostimulants to counteract abiotic and biotic stresses	1
Bioadhesive microneedle patches for tissue sealing	1
Utilization of yeasts with mannoprotein overexpression for red wine fermentation and the potential of their wine lees in wound healing	1
Metabolomics-based photosynthetic metabolic engineering	1
How the past is shaping the future of life science: The influence of automation and AI on biology	1
Comparative genomics reveals the organic acid biosynthesis metabolic pathways among five lactic acid bacterial species isolated from fermented vegetables	1
Pronounced hydroperoxide isomerase activity in Burkholderia thailandensis lipoxygenase	1
Cell therapy scale-up: bioprocess development for the production of hematopoietic cells	1
Phenolic compounds fingerprints show distinct ligninolytic bacteria behaviours during technical lignins conversion	1