New Biotechnology

Article	Citations
Bioeconomy: Biomass and biomass-based energy supply and demand	224
The role of microalgae in the bioeconomy	156
AI for life: Trends in artificial intelligence for biotechnology	142
Renewable polymers and plastics: Performance beyond the green	130
Towards a bio-based circular economy in organic waste management and wastewater treatment – The Polish perspective	72
Influence of substrate crystallinity and glass transition temperature on enzymatic degradation of polyethylene terephthalate (PET)	68
Linking the bioeconomy to the 2030 sustainable development agenda: Can SDG indicators be used to monitor progress towards a sustainable bioeconomy?	63
Development of a bioeconomy monitoring framework for the European Union: An integrative and collaborative approach	53
Comparative genomics reveals the organic acid biosynthesis metabolic pathways among five lactic acid bacterial species isolated from fermented vegetables	45
Bioeconomy moving forward step by step – A global journey	44
Bioeconomy – present status and future needs of industrial value chains	41
Explainability and causability for artificial intelligence-supported medical image analysis in the context of the European In Vitro Diagnostic Regulation	40
For the sake of the Bioeconomy: define what a Synthetic Biology Chassis is!	39
The bioeconomy in Italy and the new national strategy for a more competitive and sustainable country	38
Towards detection of SARS-CoV-2 RNA in human saliva: A paper-based cell-free toehold switch biosensor with a visual bioluminescent output	38
Sustainable production of food grade omega-3 oil using aquatic protists: Reliability and future horizons	36
Towards the sustainable production of bulk-chemicals using biotechnology	35
Biocatalysis – Key enabling tools from biocatalytic one-step and multi-step reactions to biocatalytic total synthesis	35
Bioeconomy – A driver for regional development in the Nordic countries	34
Bioeconomy development factors in the European Union and Poland	32
Two new exopolysaccharides from a thermophilic bacterium Geobacillus sp. WSUCF1: Characterization and bioactivities	28
A novel thermophile β-galactosidase from Thermothielavioides terrestris producing galactooligosaccharides from acid whey	27
Transition of microbial communities and degradation pathways in anaerobic digestion at decreasing retention time	26
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	26
Modeling the oxygen inhibition in microalgae: An experimental approach based on photorespirometry	25

Biodiplomacy, the new frontier for bioeconomy	24
A Roadmap for Industry to Harness Biotechnology for a More Circular Economy	23
Digitalization in microbiology – Paving the path to sustainable circular bioeconomy	23
Streamlined life cycle assessment of single use technologies in biopharmaceutical manufacture	23
Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer	22
Exploring Nordic microalgae as a potential novel source of antioxidant and bioactive compounds	22
Brewer’s spent grain as a no-cost substrate for polyhydroxyalkanoates production: Assessment of pretreatment strategies and different bacterial strains	21
Current state and future directions of bioeconomy in the Czech Republic	20
Rhamnolipids from non-pathogenic Acinetobacter calcoaceticus: Bioreactor-scale production, characterization and wound healing potency	20
Next biotechnological plants for addressing global challenges: The contribution of transgenesis and new breeding techniques	20
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen	20
Microalgae harvesting for wastewater treatment and resources recovery: A review	19
Synthesis and Characterization of Lignin-grafted-poly(ε-caprolactone) from Different Biomass Sources	19
Bioinformatics based discovery of new keratinases in protease family M36	19
Cryopreservation of plant cell cultures – Diverse practices and protocols	18
Point-of-care diagnosis of invasive non-typhoidal Salmonella enterica in bloodstream infections using immunomagnetic capture and loop-mediated isothermal amplification	18
The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures	18
The influence of different carbon sources on growth and single cell oil production in oleaginous yeasts Apiotrichum brassicae and Pichia kudriavzevii	17
High level production of amorphadiene using Bacillus subtilis as an optimized terpenoid cell factory	17
Significance of poly(ethylene terephthalate) (PET) substrate crystallinity on enzymatic degradation	16
Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging	16
Plant-produced bacteriocins inhibit plant pathogens and confer disease resistance in tomato	16
Poly(3-hydroxybutyrate) production from methane in bubble column bioreactors: Process simulation and design optimization	16
Genome-wide gene-deletion screening identifies mutations that significantly enhance explosives vapor detection by a microbial sensor	16
Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris	16
Techno-economic assessment of bioethanol production from lignocellulose by consortium-based consolidated bioprocessing at industrial scale	15
Whole-cell biocatalysis using the Acidovorax sp. CHX100 Δ6HX for the production of ω-hydroxycarboxylic acids from cycloalkanes	15
Synthetic activation of yeast stress response improves secretion of recombinant proteins	15
Fed-batch production assessment of a tetravalent bispecific antibody: A case study on piggyBac stably transfected HEK293 cells	14
Bioeconomy as a transforming driver of intensive greenhouse horticulture in SE Spain	14
Bioplastic production by feeding the marine Rhodovulum sulfidophilum DSM-1374 with four different carbon sources under batch, fed-batch and semi-continuous growth regimes	14
Effect of residence time in continuous photobioreactor on mass and energy balance of microalgal protein production	14
Chromate fate and effect in bioelectrochemical systems for remediation of chlorinated solvents	13
Probing the determinants of the transglycosylation/hydrolysis partition in a retaining α-l-arabinofuranosidase	13
SARS-CoV2 neutralizing activity of ozone on porous and non-porous materials	13
Effects of nutrient and oxygen limitation, salinity and type of salt on the accumulation of poly(3-hydroxybutyrate) in Bacillus megaterium uyuni S29 with sucrose as a carbon source	13
The bio-based industries joint undertaking: A high impact initiative that is transforming the bio-based industries in Europe	13
EasyMAP: A user-friendly online platform for analyzing 16S ribosomal DNA sequencing data	13
Micrometric DNA/PEI polyplexes correlate with higher transient gene expression yields in HEK 293 cells	12
The promise of explainable deep learning for omics data analysis: Adding new discovery tools to AI	12
TILAFeed: A bio-based inventory for circular nutrients management and achieving bioeconomy in future aquaponics	12
Anabolism of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator DSM 545 from spent coffee grounds oil	12
Impact of the pre-examination phase on multicenter metabolomic studies	12
A clearing protocol for Galleria mellonella larvae: Visualization of internalized fluorescent nanoparticles	12
Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome	11
Comparison of process mass intensity (PMI) of continuous and batch manufacturing processes for biologics	11
Education for a biobased economy: Integrating life and social sciences in flexible short courses accessible from different backgrounds	11
Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components	11
Combinatorial synthetic pathway fine-tuning and cofactor regeneration for metabolic engineering of Escherichia coli significantly improve production of D-glucaric acid	11
Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity	11

Glycoside Hydrolase family 30 harbors fungal subfamilies with distinct polysaccharide specificities	11
A multi-omics study to boost continuous bolaform sophorolipid production	11
In vivo evolution of lactic acid hyper-tolerant Clostridium thermocellum	11
CASPON platform technology: Ultrafast circularly permuted caspase-2 cleaves tagged fusion proteins before all 20 natural amino acids at the N-terminus	11
A fattening factor to quantify the accumulation ability of microorganisms under N-starvation	10
A blueprint from nature: miRNome comparison of plasma cells and CHO cells to optimize therapeutic antibody production	10
Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea	10
From the bacterial citrus microbiome to the selection of potentially host-beneficial microbes	10
3D printing: Economical and supply chain independent single-use plasticware for cell culture	10
Towards efficient production of highly optically pure d-lactic acid from lignocellulosic hydrolysates using newly isolated lactic acid bacteria	9
Opinion: Independent third-party entities as a model for validation of commercial antibodies	9
Porcine circovirus 2 capsid protein produced in N. benthamiana forms virus-like particles that elicit production of virus-neutralizing antibodies in guinea pigs	9
Influence of culture media composition on the rheology of microalgae concentrates on a large scale	9
Thauera sp. Sel9, a new bacterial strain for polyhydroxyalkanoates production from volatile fatty acids	9
CRISPR-Cas12a assisted precise genome editing of Mycolicibacterium neoaurum	9
Enzymatic synthesis of wet-resistant lignosulfonate-starch adhesives	9
High-throughput screening for heterotrophic growth in microalgae using the Biolog Plate assay	8
Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies	8
Using galactitol dehydrogenase coupled with water-forming NADH oxidase for efficient enzymatic synthesis of L-tagatose	8
A practical approach for modelling the growth of microalgae with population balance equation	8
Imaging of individual transcripts by amplification-based single-molecule fluorescence in situ hybridization	8
Nonionic detergent micelle aggregates: An economical alternative to protein A chromatography	8
An artificial intelligence approach for identification of microalgae cultures	8
The PAR promoter expression system: Modified lac promoters for controlled recombinant protein production in Escherichia coli	8
Closing the loop in bioproduction: Spent microbial biomass as a resource within circular bioeconomy	8
A novel high-throughput approach for transforming filamentous fungi employing a droplet-based microfluidic platform	8
Bioeconomy triple factor nexus through indicator analysis	8
Food biopharmaceuticals as part of a sustainable bioeconomy: Edible vaccines case study	7
Bioeconomy in Russia: Today and tomorrow	7
A recombinant strain of Komagataeibacter xylinus ATCC 23770 for production of bacterial cellulose from mannose-rich resources	7
Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production	7
Accelerostat study in conventional and microfluidic bioreactors to assess the key role of residual glucose in the dimorphic transition of Yarrowia lipolytica in response to environmental stimuli	7
Iron availability enhances the cellular energetics of aerobic Escherichia coli cultures while upregulating anaerobic respiratory chains	7
Development of antibodies against the notch ligand Delta-Like-1 by phage display with activity against breast cancer cells	7
A single-chain variable fragment selected against a conformational epitope of a recombinantly produced snake toxin using phage display	7
Bioprocess intensification: Cases that (don’t) work	7
FN3-based monobodies selective for the receptor binding domain of the SARS-CoV-2 spike protein	7
Structural characterization of the family GH115 α-glucuronidase from Amphibacillus xylanus yields insight into its coordinated action with α-arabinofuranosidases	7
Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater	7
Identification of lipolytic enzymes using high-throughput single-cell screening and sorting of a metagenomic library	7
Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress	7
Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control	6
Applicability and information value of biocalorimetry for the monitoring of fungal solid-state fermentation of lignocellulosic agricultural by-products	6
A unique biochemical reaction pathway towards trehalulose synthesis by an amylosucrase isolated from Deinococcus deserti	6
Peptone from casein, an antagonist of nonribosomal peptide synthesis: a case study of pedopeptins produced by Pedobacter lusitanus NL19	6
Adaptive laboratory evolution to hypersaline conditions of lactic acid bacteria isolated from seaweed	6
Combination of sodium butyrate and decitabine promotes transgene expression in CHO cells via apoptosis inhibition	6
Enhancement of a biotechnological platform for the purification and delivery of a human papillomavirus supercoiled plasmid DNA vaccine	6
Engineering a heterologously expressed fructosyltransferase from Aspergillus oryzae N74 in Komagataella phaffii (Pichia pastoris) for kestose production	6
Introducing molasses as an alternative feedstock into itaconate production using Ustilago sp	6
Easily applicable modifications to electroporation conditions improve the transformation efficiency rates for rough morphotypes of fast-growing mycobacteria	6
Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase	6
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	6
Standardization of generic pre-analytical procedures for in vitro diagnostics for personalized medicine	6
Pre-analytical sample stabilization by different sampling devices for PCR-based COVID-19 diagnostics	6
External Quality Assurance programs for processing methods provide evidence on impact of preanalytical variables	6
Derivation of splice junction-specific antibodies using a unique hapten targeting strategy and directed evolution	5
pCAT vectors overcome inefficient electroporation of Cupriavidus necator H16	5
Machine translation of standardised medical terminology using natural language processing: A scoping review	5
Food and human health applications of edible mushroom by-products	5
Extracellular and intracellular phenol production by microalgae during photoautotrophic batch cultivation	5
Development of anti-human IgM nanobodies as universal reagents for general immunodiagnostics	5
Proper definition of the set of autoantibody-targeted antigens relies on appropriate reference group selection	5
Start-up control for nitrogen removal via nitrite under low temperature conditions for swine wastewater treatment in sequencing batch reactors	5
A wide array of lignin-related phenolics are oxidized by an evolved bacterial dye-decolourising peroxidase	5
GH10 and GH11 endoxylanases in Penicillium subrubescens: Comparative characterization and synergy with GH51, GH54, GH62 α-L-arabinofuranosidases from the same fungus	5
Deciphering integration loci of CHO manufacturing cell lines using long read nanopore sequencing	5
Domain shifts in dermoscopic skin cancer datasets: Evaluation of essential limitations for clinical translation	5
Purification of DNA oligonucleotides to improve hybridization chain reaction performance	5
Combinatorial mutagenesis with alternative CDR-L1 and -H2 loop lengths contributes to affinity maturation of antibodies	5
Detection of SARS-CoV-2 antibodies in serum and dried blood spot samples of vaccinated individuals using a sensitive homogeneous proximity extension assay	4
Novel target capture DNA library preparation method using CircLigase-mediated hook ligation	4
The Jo-In protein welding system is a relevant tool to create CBM-containing plant cell wall degrading enzymes	4
Introduction to the special issue: Trends in bioeconomy	4
New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria	4
Automatable downstream purification of the benzohydroxamic acid D-DIBOA from a biocatalytic synthesis	4
Personalized 3D printed scaffolds: The ethical aspects	4
Legal and practical challenges to authorization of gene edited plants in the EU	4
EBRC: Enhancing bioeconomy through research and communication	4
CaRA – A multi-purpose phage display library for selection of calcium-regulated affinity proteins	4

A new control strategy to improve the mass transfer capacity and reduce air injection costs in raceway reactors	4
Impact of standardization in tissue processing: the performance of different fixatives	4
An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics	4
Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells	4
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	4
Towards a bioeconomic vision for New Zealand – Unlocking barriers to enable new pathways and trajectories	4
Rheology of microalgae concentrates and its influence on the power consumption of enzymatic hydrolysis processing	4
Structure of fungal communities in sequencing batch reactors operated at different salinities for the selection of triacylglyceride-producers from a fish-canning lipid-rich waste stream	4
A cascade reaction for the synthesis of d-fagomine precursor revisited: Kinetic insight and understanding of the system	4
Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors	4