New Biotechnology

Article	Citations
Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system	309
EBRC: Enhancing bioeconomy through research and communication	139
A unique class I polyhydroxyalkanoate synthase (PhaC) from Brevundimonas sp. KH11J01 exists as a functional trimer: A comparative study with PhaC from Cupriavidus necator H16	86
Tailored enzyme expression modifies Shewanella oneidensis biofilms and increases current density	82
MutS-mediated rapid and cost-effective error correction in in vitro DNA synthesis	77
Enzymatic degradation of biopolyesters as a key strategy for bioplastic circularity	68
Production of bacterial cellulose by Komagataeibacter nataicola NRIC 0616 on various culture media	66
Plant-based production of oral vaccines for sustainable fish aquaculture	57
Microbial synthesis of cell penetrating peptides to facilitate the active principle penetration in cosmetics	48
Basic plant research in service of crop breeding – the Application Laboratory for Agricultural Research in Czech Republic	48
Advancing fracture repair: gelatin-starch adhesive technique	41
Identification and biosynthesis of an autoinducing cyclopeptide from Clostridium acetobutylicum that regulates solvent biosynthesis and cell morphology	41
Structure-function landscape models: a new avenue towards the precision engineering of microbial consortia	37
Co-liquefaction of oleaginous yeast and algal biomass: strategic enhancement of bio-crude oil productivity	36
Whole-cell bacterial biosensors for detection of structurally-diverse chemicals	34
OncoFlow: A multiplexed microfluidic platform for personalized drug sensitivity assessment	34
A multi-omics study to boost continuous bolaform sophorolipid production	33
Successful combination of lactic acid bacteria and yeast fermentation and enzymatic treatment to re-cycle industrial bread by-products for bread making	32
3D printing: Economical and supply chain independent single-use plasticware for cell culture	28
Viability assessment and biomass valorization of recombinant Komagataella phaffii	27
Formation of biologically influenced palladium microstructures by Desulfovibrio desulfuricans and Desulfovibrio ferrophilus IS5	26
A computational-guided framework and versatile assembly method for genome-wide CRISPRi downregulation in metabolic engineering studies	26
Optimisation of rhamnolipids production using response surface methodology	25
Simultaneous saccharification and fermentation for D-lactic acid production at high temperature by a thermally-adapted homolactogenic Escherichia coli strain	24
Recombinant production of bovine serum albumin (BSA) in yeast: optimization and enhanced blocking capacity in immunoassays	24

Improving CRISPR-Cas9 mediated genome integration in interspecific hybrid yeasts	24
Balancing act: improving the environmental and economic performance of industrial-scale basic oxygen furnace gas fermentation for isopropyl alcohol production	23
Recombinant production of a killer yeast toxin in Pichia pastoris and Bacillus subtilis	23
Sustainable solutions: maximizing resources using agro-industrial by-products for dairy products fortification	23
Repurposing starchy potato washing slurries and footwear dust in the development of 3D bioplastic packaging - a circular economy approach	22
Phenoplate: An innovative method for assessing interacting effects of temperature and light on non-photochemical quenching in microalgae under chemical stress	21
Assessing the potential of olive mill solid waste as feedstock for methane and volatile fatty acids production via anaerobic bioprocesses	21
Automating the design-build-test-learn cycle towards next-generation bacterial cell factories	21
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein	21
Downstream process for the purification of biotechnologically produced D-DIBOA	20
Human biological system mimic nano-complexation for pharmaceuticals/cosmetics/functional foods	20
Microbial conversion of waste algal biomass to zeaxanthin by using Cellulophaga omnivescoria MSK1 strain	20
Ene reductase catalysed formation of chiral carbon(sp3)-sulfur (II) centres	20
Strategies for the lipidation of bioactive molecules: chemo-enzymatic synthesis of epoxidized fatty acids and their functionalization	20
Is human oversight to AI systems still possible?	20
Biotechnological production of valuable monomers and biopolymers from textile waste blends	20
Identification of halophilic phage genomes from a solar saltern through shotgun sequencing: a metagenomic study	20
Using control bias to identify initial targets for bioproduction improvement	20
Highly specific metal-binding peptides for selective resource recovery from spent lithium-ion batteries	19
Engineering of NAD-dependent dehydrogenases to change cofactor specificity towards NMN	19
Nucleic acid aptamers protect against lead (Pb(II)) toxicity	18
Protein coding regions prediction by fusing DNA shape features	18
The evolution of reproductive mode in the Carassius auratus complex	18
Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers	18
CO2 and H2-based bio-hexanol production process development with an adapted strain of Clostridium carboxidivorans	18
Discovery of a new class of bacterial heme-containing C C cleaving oxygenases	17
A COST Action on microbial responses to low pH: Developing links and sharing resources across the academic-industrial divide	17
Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast	16
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential	16
Efficient surface display of single-chain variable fragments against tumor necrosis factor α on engineered probiotic Saccharomyces boulardii and its application in alleviating intestinal inflammation	16
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source	16
Post-translational secretion stress regulation in Bacillus subtilis is controlled by intra- and extracellular proteases	16
Physiology of ethanol-grown Cyberlindnera jadinii: towards sustainable protein production	15
Comparative transcriptomics of CAZy enzymes in white- and brown-rot agaricomycetes: Evolutionary insights into lignocellulose degradation and the relevance of GH16 glycoside hydrolase functional diver	15
A Nakaseomyces glabratus CRISPRi platform to study the mode of action of novel antifungals	14
Biotechnological potential of amphisin produced on black cumin oilseed cake	14
Beyond iron: harnessing siderophores for the comprehensive mobilization of essential soil elements	14
Determination of the oxygen mass transfer coefficients in a bioreactor with mechanical mixing for Poly(3-Hydroxybutyrate) production	14
Monitoring metagenomes using long-read nanopore sequencing	14
From the discovery of novel enzymes to their synthetic application: investigating and harnessing natural biodiversity toward efficient and selective bioprocesses	13
Enhancing sequestration of heavy metals using exopolysaccharides produced by cyanobacteria grown at ultra-high CO2 levels	13
Arsenic removal process from acid mine drainage using bacteria	13
A search for new compounds for candidosis treatment – screening of Antarctic bacteria from the IBA collection	13
Synthetic biology of acetogenic bacteria for sustainable C1 gas bioconversion	13
Future food court in an urban shopping center can supply feedstocks for microbial protein production	12
Knock-out of the major regulator Flo8 in Komagataella phaffii results in unique host strain performance for methanol-free recombinant protein production	12
Enhancing enzymatic rhamnolipid synthesis: comparative analysis of sustainable energy sources	12
Establishment of an RNA-based transient expression system in the green alga Chlamydomonas reinhardtii	12
Extraction and properties of protein extracted from fungal fermentations	12
Biostimulation effect of different amendments on Cr(VI) recovering microbial community	12

Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea	12
Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors	11
Systematic identification of genomic hotspots for high-yield protein production in CHO cells	11
Immobilization of His6-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes	11
A fattening factor to quantify the accumulation ability of microorganisms under N-starvation	11
Earth: Extinguishing anthropogenic risks through harmonization	11
Exploration and structure-based engineering of alkenal double bond reductases catalyzing the Cα Cβ double bond reduction of coniferaldehyde	11
In vivo evolution of lactic acid hyper-tolerant Clostridium thermocellum	10
XPORT ENTRAP: A droplet microfluidic platform for enhanced DNA transfer between microbial species	10
Optimizing ferritin nanoparticle production for enhanced antigen presentation in vaccine development	10
Evaluation and optimization of CO2 capture and biomass valorization into value-added products by the green non-sulfur bacteria Chloroflexus aurantiacus	9
Energy-efficient clostridial butanol production via histidine kinase regulation to improve strain tolerance and process robustness	9
3-hydroxypropionic acid production in engineered Cupriavidus necator H16	9
Conversion of food processing residues into sustainable soil improvers by using enzymatic hydrolysis	9
Exploring the importance of palmitoylation: alkyl chain length of lipidated proteins affects their interaction with lipid membranes and vesicular transport	9
Bacterial microcompartments: the new biotechnological tool for maximizing catabolic pathway efficiency in microorganisms	9
Utilising a genetically encoded biosensor for in-line monitoring of H2O2 generation during methanol assimilation in the yeast Komagataella phaffii	9
Enzymatic recycling of polyhydroxyalkanoate (PHA) bioplastics using PHA depolymerases	9
De novo production of valuable aromatic aldehydes and aldehyde-derived products by engineered solvent-tolerant and oxidation-deficient Pseudomonas taiwanensis VLB120	9
Upgraded activity assays for PET-degrading enzymes	9
Towards a fungal-based colorful future: upscale of colorant production from several fungal species isolated from Dutch parks	9
Valorisation of wastewater treatment sewage scum through the production of biofuels	9
Development of chemically defined, DMSO-free cryopreservation media for mammalian cells	9
Intensified butanol production through optimisation of integrated membrane bioreactor operation	9
A cell-free high throughput assay for assessment of SARS-CoV-2 neutralizing antibodies	8
Single-cell proteins polyhydroxyalkanoates-rich microbial biomass from municipal and winery waste as potential additive for aquafeeds	8
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365	8
Bacterial cell immobilization using the miniaturized adhesive protein AtaA for efficient bioprocesses	8
An approach to rapid distributed manufacturing of broad spectrum anti-viral griffithsin using cell-free systems to mitigate pandemics	8
Prenylation of diverse indole derivatives by the fungal aromatic prenyltransferase RePT	8
Development of integrated biofoundry for productions of fuels and chemicals from CO2 and unutilized biomass	8
Valorization of bioethanol by-products to produce unspecific peroxygenase with Agrocybe aegerita: Technological and proteomic perspectives	8
Valorization of prosecco wine lees for sustainable polyhydroxyalkanoates production by Cupriavidus necator DSM 545 and Hydrogenophaga pseudoflava DSM 1034	8
In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions	8
Biodegradable zein/PEG nanofibers incorporated with natural antimicrobial compounds for eco-friendly food packaging	8
Treatment of real oilfield produced wastewater by ionizing radiation: disinfection and changes in bacterial community and predictive function	7
Discovering novel bacterial biosynthetic potential from draft genomic data	7
The pretreatment of sugar, calcium and vitamin C to enhance the color, texture and flavor properties of frozen avocado	7
Fungal G-protein coupled receptors (GPCRs): potential novel drug targets?	7
A counter-selection system exploiting phenotypic heterogeneity for improved production of glutathione from yeast	7
Catabolism and production of sugar acids by Corynebacterium glutamicum	7
Anti-biofilm molecules from Antarctic marine bacteria as weapons against microbial infections	7
Comparison of alpha-glucuronidase production by E. coli HMS174(DE3) and BL21(DE3)	7
Surface layer structure of the periodontal health-associated bacterium Tannerella serpentiformis	7
Unlocking the potential of bioproducts from waste streams for soil restoration	7
Carotenoids-based biohybrid perovskite solar cells mimicking natural photosystem.	7
A versatile odor detection system based on automatically trained rats for chemical sensing	7
Bioanalytical technologies for equitable women’s healthcare: development of ultrasensitive assays and bioinspired nanomaterials for biomarker-based diagnostics	7
Improved taxadiene production by optimizing DXS expression and fusing short-chain prenyltransferases	6
Development of a bioprocess for the production of a herbicidal sugar as sustainable alternative to glyphosate	6
Autotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness	6
N-arylated 1,4-diazabicyclo[2.2.2]octane quaternary ammonium salts as a new approach for thiol and selenol luminescent probes design	6
Transgenesis in pea (Pisum sativum L.) and flax (Linum usitatissimum L.): Agritec´s experience	6
Engineering synthetic metabolons using self-assembled protein scaffolds	6
Plastics active enzymes for polyolefine and polyester degradation and derivatization	6
Influence of biochar on biohydrogen production in dark fermentation	6
Investigating and comparing dihydrogen thresholds and consumption kinetics of different methanogenic species	6
Significance of poly(ethylene terephthalate) (PET) substrate crystallinity on enzymatic degradation	6
Human milk oligosaccharides: high-yield production of a new food ingredient	6
Impact of standardization in tissue processing: the performance of different fixatives	6
Unraveling the proteomics landscape of medium composition in the model biodesulfurization strain Rhodococcus qingshengii IGTS8	6
Modeling of a bioreactor with immobilized laccase for antibiotic removal considering the effects of diffusion-convection-reaction	6
From methanol to medicine: sustainable bioproduction of polyketides	5
Semi-continuous cultivation of EPS-producing marine cyanobacteria: A green biotechnology to remove dissolved metals obtaining metal-organic materials	5
Evaluation of metallurgical dust recycling applying concepts of bioleaching	5
Green physical processes for the extraction of organic dye betacyanin from dragon fruit (Hylocereus purpusii) peels	5
Computer-aided characterization and evaluation of enzymes active sites	5
Engineering enhanced signal peptides: A high-throughput computational pipeline for optimizing therapeutic protein production in CHO cells	5
Bioinformatics based discovery of new keratinases in protease family M36	5
Bioactive steroids from cortisone bioconversion: antioxidant properties in ocular model	5
Multi-enzymatic approaches involving engineered D-amino acid dehydrogenases for the stereoselective synthesis of D-phenylalanines	5
Exploring yeast diversity for ethanol-fueled industrial biotechnology	5
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets	5
Antibody-oligonucleotide conjugation based on cyanine dyes as cross-linking reagents	5
Getting a grip on oxygen transfer in bioreactors: automated kLa determination using the software Lucullus®	5
C-glycosyltransferase discovery and engineering for the synthesis of bioactive glycosides	5
Transcription factor-based biosensors for detection of naturally occurring phenolic acids	4
Functional characterization of top-down community assembly in a simple environment	4
Upcycling cotton textile waste into lactic acid by chemo-mechanical pretreatment, separate hydrolysis and fermentation with engineered Saccharomyces cerevisiae strain	4
Isolation and characterization of novel proteins from octopus	4

Effect of operating conditions on the microbial selection of P(3HB-co-3HV) producers in open continuous culture	4
Cultivation at a liquid–liquid interface prevents phenotypic heterogeneity of human induced pluripotent stem-derived retinal pigment epithelial cells	4
Cultivation optimization promotes ginsenoside and universal triterpenoid production by engineered yeast	4
Lipopeptide production from xylose and its action in rheology modification for potential industrial application	4
Efficient full-length IgG secretion and sorting from single yeast clones in droplet picoreactors	4
ReViTA: A novel in vitro transcription system to study gene regulation	4
Soft sensor for viable cell counting by measuring dynamic oxygen uptake rate	4
The microalgal sector in Europe: Towards a sustainable bioeconomy	4
Cell-free synthesis of human norovirus capsid protein VP1	4
Metabolic regulation by the chromatin remodeler Ino80 under stress conditions and improvement of yeast stress tolerance for efficient bioproduction	4
Utilising CO2 as a feedstock for renewable chemicals: biocatalytic production of L-lactic acid from CO2 and ethanol	4
Precision fermentation and downstream processing of fungal colorants	4
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: The periodic hydrodynamic loading and the shear stress accumulation.	4
Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production	4
Effect of the stringent response on amylase production and the ribosome profile of Bacillus subtilis	4
Unlocking the power of Moringa oleifera: a surprising synergy with cereal-based products	3
Exosome-like nanoparticles from Arbutus unedo L mitigate LPS-induced inflammation via JAK-STAT inactivation	3
Diversifying bioactive natural products: prenylation of plant phenolics and indole derivatives by fungal prenyltransferases	3
Engineering Pseudomonas putida for bioproduction of alkylamines	3
New vectors for urea-inducible recombinant protein production	3
Pairwise residues coevolution of Candida antarctica lipase B for efficient synthesis of δ-dodecalactone	3
Gene essentiality in the solventogenic Clostridium acetobutylicum DSM 792	3
Caffeine-regulated molecular switches for functional control of CAR T cells	3
Biotechnological recovery of critical raw materials from end-of-life lithium-ion batteries	3
Production of nicotinamide mononucleotide by high cell density culture of metabolically engineered Escherichia coli	3
Improving the efficiency of chemical production from one-carbon substrates in Komagataella phaffii through enzyme and metabolic engineering	3
Production of a plant-based vaccine candidate against COVID-19 through molecular pharming	3
Enhancing 2,3-butanediol and acetoin production from brewer's spent grain hemicellulosic hydrolysate through bacterial co-cultivation	3
Designing a hybrid integrated bottled photobioreactor system for enhanced biomass yield and CO2 fixation during Limnospira fusiformis cultivation	3
Targeting enzymes for plastic degradation: developing Affimers as novel plastic-binding modules	3
Enzymatic production of (S)-citronellol from citral using an Old Yellow Enzyme and a short-chain alcohol dehydrogenase	3
Biotechnology applied to soybean cultivation: the use of microorganisms to increase drought tolerance	3
Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components	3
Multifunctional bioplastic coating derived from starch-rich potato washing slurries and tomato pomace for components of footwear	3
Upcycling plastic waste: microbial synthesis of bulk chemicals from poly(ethylene terephthalate) (PET)	3
Development of a self-powered electric and UV disinfection system based on a microbial electrochemical system	3
Plant expression systems as an economical alternative for the production of iELISA coating antigen AHSV VP7	3
Biosynthesis of biodegradable plastic, poly(3HB-co-3HP), using methane as sole carbon source in metabolically engineered type II methanotroph, Methylosinus trichosporium OB3b	3
Bacillus thuringiensis strain with the transcriptional fusion phaR-GFP and Acetyl CoA-GFP promotor regions to assess bioplastic accumulation	3
Recovery of C6 carboxylate from CO₂-based microbial electrosynthesis	3
A systems-view and optimization of bacteria with synthetic reductive glycine pathway for C1-assimilation	2
Serotonin overproduction in Saccharomyces cerevisiae: a metabolic engineering approach	2
Gramicidin S production through exclusive use of agrifood byproducts as nutritional sources	2
Editorial Board	2
Utilization of lactose and whey permeate for the sustainable production of polyhydroxyalkanoates by Hydrogenophaga pseudoflava DSM1034	2
Explainability and causability for artificial intelligence-supported medical image analysis in the context of the European In Vitro Diagnostic Regulation	2
Immobilization of Dietzia sp. KDB1 bacteria on electrospun fiber mats for indigo reduction	2
Antifungal activity of biosurfactants against plant pathogenic fungi	2
Advancements in flavonoid glycosylation: unlocking bioproduction potential with Escherichia coli W as novel platform for large-scale production	2
Conversion of syngas-derived C2 carbon sources to C2+ biochemicals by microbial cell factories	2
A gradient phage-assisted continuous evolution method for screening suppressor tRNAs in Escherichia coli	2
An engineered SARS-CoV-2 receptor-binding domain produced in Pichia pastoris as a candidate vaccine antigen	2
Domain shifts in dermoscopic skin cancer datasets: Evaluation of essential limitations for clinical translation	2
Influence of whey protein on black soybean anthocyanins’ thermostability and inhibitory effect toward xanthine oxidase	2
Virtual screening of bioactive peptides with antimicrobial activity targeting carbonic anhydrase II from Escherichia coli	2
Fungal waste treats water waste: enzymatic and non-enzymatic removal of organic micropollutants with spent mushroom substrate of Agaricus bisporus	2
Editorial Board	2
Production and characterization of novel Anti-HIV Fc-fusion proteins in plant-based systems: Nicotiana benthamiana & tobacco BY-2 cell suspension	2
Shedding light on the black box of a neural network used to detect prostate cancer in whole slide images by occlusion-based explainability	2
Going beyond the limit: engineering mRNA decay and protein translation initiation enhances recombinant protein secretion	2
Enzyme design and engineering for efficient biosynthesis of high-value compounds	1
Key challenges in establishing a commercial fermentative production process of the steviol glycosides rebaudioside M and D	1
Study on the effect of high-hydrostatic-pressure treatment on vegetable green soybean in reducing blood lipids in high-fat diet-induced obese mice	1
Optimizing RuBisCO catalytic activity through active-site loop stabilization	1
In vitro protein synthesis versus E. coli expression - a comparison using the acetate transporter SatP	1
Strategic improvement of Shewanella oneidensis for biocatalysis: Approach to media refinement and scalable application in a microbial electrochemical system	1
Reducing cellulases production cost through systematically optimizing submerged fermentation of Trichoderma reesei	1
Effects of zingerone nanoparticles on anti-angiogenesis in vitro and in vivo	1
Tailoring Structure-Property Relationships of Fungal Mycelium for Material Applications: A Process Engineering Approach for Pure Mycelium-Based Biomaterials	1
Exploring Nordic microalgae as a potential novel source of antioxidant and bioactive compounds	1
Site-specifically immobilized D-amino acid dehydrogenase for the synthesis of D-phenylalanine	1
Bioprospecting fungal strains from Finnish forests for possible industrial applications through biocatalysis	1
Advancing biobased product development from diverse bioresources through customized designer bioactivities	1
Producing food from acetate via fermentation	1
Large genome Serratia endophytes boost nutrient content and extend shelf life of tomatoes in agricultural fields	1
Phytoremediation of diclofenac using the Green Liver System: Macrophyte screening to system optimization	1
Virus inactivation using an electrically conducting virus filter in biopharmaceutical manufacturing process	1
Identification and expression of novel polyester-degrading enzymes for application on mixed plastic-containing waste	1
Continuous VFA production from lignocellulosic biomass via an artificial rumen reactor and membrane filtration: the cow as inspiration	1
Reengineering of ergosterol biosynthesis in Saccharomyces cerevisiae and its sustainable application in citrus peel processing industry	1
Enhancing protein productivities in CHO cells through adenosine uptake modulation – Novel insights into cellular growth and productivity regulation	1
Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content	1
P. pastoris (K. phaffi) perfusion processes: evaluation of cell retention techniques and integrated purification strategies	1
Recovering pathogenicity by reintroduction of a putative transcriptional regulator into a non-pathogenic Botrytis cinerea isolate	1
Use of hollow-fibre membranes for minimizing oxygen entry into anaerobic chemostat cultures of yeasts	1
Boosting photosynthesis to deliver novel crops for the circular bioeconomy	1
How food wastes can be converted into new products: European legislation and analysis of enzymatic hydrolysis	1
Establishment of a cell-free multi-enzyme cascade for the synthesis of UDP-GalNAc	1
SARS-CoV2 neutralizing activity of ozone on porous and non-porous materials	1
Applying a transaminase for the sustainable production of 5-hydroxymethylfurfurylamine using cadaverine	1
Glucose to nitrogen ratio effect for phycocyanin productivity under hetero- and mixotrophic conditions with the microalga Galdieria sulphuraria	1
A hitchhiker’s guide to stable co-cultures for industrial biotechnology	1
A unique biochemical reaction pathway towards trehalulose synthesis by an amylosucrase isolated from Deinococcus deserti	1
Engineering a heterologously expressed fructosyltransferase from Aspergillus oryzae N74 in Komagataella phaffii (Pichia pastoris) for kestose production	1
Metabolomics uncovers the significance of metabolites in the antioxidant properties and quality characteristics of Mussaenda pubescens	0