Altex-Alternatives to Animal Experimentation

Article	Citations
Alternative Methods in Science: Towards Fluidic Systems	61
Application of high-throughput transcriptomics for mechanism-based biological read-across of short-chain carboxylic acid analogues of valproic acid_suppl1	37
Intestinal in vitro transport assay combined with physiologically based kinetic modeling as a tool to predict bile acid levels in vivo_suppl1	32
Determining a point of departure for skin sensitization potency and quantitative risk assessment of fragrance ingredients using the GARDskin dose-response assay_suppl1	25
Systematic analysis of read-across adaptations in testing proposal evaluations by the European Chemicals Agency_suppl5	21
Sensitivity analysis and quality indicators for an in vitro oral irritation assay	12
Validation of an Endopep-suspension immunoassay for the diagnostics of human botulism_suppl	11
Systematic analysis of read-across adaptations in testing proposal evaluations by the European Chemicals Agency	11
The effect of surfactants and film-forming polymers on pulmonary surfactant function measured in vitro is dose rate dependent_suppl	11
Animal research ethics as interaction of research ethics, animal ethics, and (animal protection) law	10
Validation of the monocyte activation test with three therapeutic monoclonal antibodies	9
REACH out-numbered! The future of REACH and animal numbers	8
Integrated skin sensitization assessment based on OECD methods (I): Deriving a point of departure for risk assessment	8
Developmental neurotoxicity (DNT): A call for implementation of new approach methodologies for regulatory purposes: Summary of the 5th International Conference on DNT Testing	8
Technical framework for enabling high-quality measurements in new approach methodologies (NAMs)	8
Off to a good start? Review of the predictivity of reactivity methods modelling the molecular initiating event of skin sensitization	8
Integrated skin sensitization assessment based on OECD methods (I): Deriving a point of departure for risk assessment_suppl4	8
Predicting Acute Oral Toxicity Using AcutoX: An Animal Product-Free and Metabolically Relevant Human Cell-Based Test	7
Making safety decisions for a sunscreen active ingredient using next-generation risk assessment: Benzophenone-4 case study	7
A sensitive cell-based assay for testing potency of Botulinum neurotoxin type A	7
The long way from raw data to NAM-based information: Overview on data layers and processing steps	7
Beyond Animal Testing Index: Benchmarking tool for a world beyond animal testing_suppl4	7
Mapping out strategies to further develop human-relevant, new approach methodology (NAM)-based developmental neurotoxicity (DNT) testing	7
E-validation – Unleashing AI for validation	7
Chemical selection for the Thyroid Validation Study coordinated by EURL ECVAM and involving EU-NETVAL laboratories_suppl3	6

Microphysiological endothelial models to characterize subcutaneous drug absorption_suppl	6
Exploring the synergy of CRISPR and microphysiological systems	6
Protectiveness of NAM-based hazard assessment – which testing scope is required?_suppl1	6
Mapping physiology: A systems biology approach for the development of alternative methods in toxicology	6
Developing a global education hub for animal-free innovation_suppl	6
Non-animal approaches – Concept, validation, and regulatory acceptance: The fourth Asian Congress of Alternatives to Animal Experiments (4ACAAE) and the seventh Annual Meeting of the Society for Alter	5
3Rs: Progress or a fig leaf?	5
Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity	5
Systematic analysis of read-across adaptations in testing proposal evaluations by the European Chemicals Agency_suppl6	5
What does “success” look like in compliance check decisions by the European Chemicals Agency? The curious cases of accepted read-across adaptations	5
Systematic analysis of read-across adaptations in testing proposal evaluations by the European Chemicals Agency_suppl2	5
Investigation of the publication rate of recent research projects using non-human primates in France	5
COVID-19 through Adverse Outcome Pathways: Building networks to better understand the disease – 3rd CIAO AOP Design Workshop	5
A human osteoarthritis mimicking goat cartilage explant-based disease model for drug screening_suppl	5
AOP 460: Antagonism of Smoothened receptor leading to orofacial clefting_suppl1	5
Designing a national strategy to enable human-relevant research in India: A multistakeholder roundtable meeting report	4
In vitro-based prediction of human plasma concentrations of food-related compounds_suppl1	4
ALTEX Proceedings TIERethik Corrigendum to Incorporating new approach methodologies into regulatory nonclinical pharmaceutical safety assessment	4
Incorporating new approach methodologies into regulatory nonclinical pharmaceutical safety assessment	4
The Alarming Consequences of Workforce Reductions at the FDA, EPA, NIH and CDC in the United States	4
Determining a point of departure for skin sensitization potency and quantitative risk assessment of fragrance ingredients using the GARDskin dose-response assay_suppl3	4
Integrated skin sensitization assessment based on OECD methods (II): Hazard and potency by combining kinetic peptide reactivity and the “2 out of 3” Defined Approach_suppl4	4
Role of standards and funding in accelerating the development and use of microphysiological systems	4
Material-mediated pyrogens in medical devices: Myth or reality?_suppl2	4
Determining a point of departure for skin sensitization potency and quantitative risk assessment of fragrance ingredients using the GARDskin dose-response assay	4
The Virtual Human Platform for Safety Assessment (VHP4Safety) project: Next generation chemical safety assessment based on human data	4
Assessment of developmental neurotoxicology-associated alterations in neuronal architecture and function using Caenorhabditis elegans_suppl	4
From cellular perturbation to probabilistic risk assessments	4
Women in Alternatives	4
Linking nanomaterial-induced mitochondrial dysfunction to existing adverse outcome pathways for chemicals_suppl1	3
A path forward advancing microphysiological systems	3
Transition to animal-free science: Phasing out animal experiments, phasing in innovation	3
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl2	3
Beyond chemicals: Opportunities and challenges of integrating non-chemical stressors in adverse outcome pathways	3
COVID-19 through Adverse Outcome Pathways: Building networks to better understand the disease – 3rd CIAO AOP Design Workshop_suppl	3
Human relevant frontiers in drug safety and efficacy	3
Identifying candidate reference chemicals for in vitro testing of the retinoid pathway for predictive developmental toxicity_suppl	3
Grouping of UVCB substances with dose-response transcriptomics data from human cell-based assays_suppl	3
Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis_suppl1	3
Qualitative and quantitative concentration-response modelling of gene co-expression networks to unlock hepatotoxic mechanisms for next generation chemical safety assessment	2
The SCAHT Adverse Outcome Pathway (AOP)_HUB: A hands-on platform for information exchange, sharing, and developing AOPs	2
Integrated skin sensitization assessment based on OECD methods (II): Hazard and potency by combining kinetic peptide reactivity and the “2 out of 3” Defined Approach_suppl2	2
Proceedings of a workshop to address animal methods bias in scientific publishing_suppl	2
Next-generation risk assessment of chemicals – Rolling out a human-centric testing strategy to drive 3R implementation: The RISK-HUNT3R project perspective	2
Linking nanomaterial-induced mitochondrial dysfunction to existing adverse outcome pathways for chemicals_suppl2	2
Impact of in vitro experimental variation in kinetic parameters on physiologically based kinetic (PBK) model simulations_suppl1	2
Second scientific sessions and international conference of the Society for Alternatives to Animal Testing in Sri Lanka (SAAT-SL)	2
What does “success” look like in compliance check decisions by the European Chemicals Agency? The curious cases of accepted read-across adaptations_suppl6	2
How to formulate hypotheses and IATA to support grouping and read-across of nanoforms	2
Building confidence in PBK model predictions in the absence of human kinetic data: Benzophenone-4 case study_suppl1	2

The need for epigenotoxicity testing	2
Development of a network of carcinogenicity adverse outcome pathways and its employment as an evidence framework for safety assessment	2
Virtual control groups in non-clinical toxicology – A replicability challenge	2
Micro-replace systems	2
Biology-inspired dynamic microphysiological system approaches to revolutionize basic research, healthcare and animal welfare	2
What does “success” look like in compliance check decisions by the European Chemicals Agency? The curious cases of accepted read-across adaptations_suppl7	2
Comparative evaluation of rat and human in vitro assays for evaluation of thyroid toxicity_suppl1	2
Novel prediction models for genotoxicity based on biomarker genes in human HepaRGTM cells_suppl2	2
AOP 460: Antagonism of Smoothened receptor leading to orofacial clefting	2
Open-source human skin model with an in vivo-like barrier for drug testing_suppl	2
A human iPSC-based in vitro neural network formation assay to investigate neurodevelopmental toxicity of pesticides_suppl2	2
Grouping of UVCB substances with dose-response transcriptomics data from human cell-based assays	1
Towards NAM-based risk assessment for developmental neurotoxic effects, illustrated with chlorpyrifos	1
Integrated skin sensitization assessment based on OECD methods (I): Deriving a point of departure for risk assessment_suppl3	1
Ready for regulatory use: NAMs and NGRA for chemical safety assurance	1
Predictive performance of next generation human physiologically based kinetic (PBK) model predictions based on in vitro and in silico input data_suppl2	1
On the usefulness of animals as a model system (part II): Considering benefits within distinct use domains	1
Building confidence in PBK model predictions in the absence of human kinetic data: Benzophenone-4 case study	1
Exploration of the GARDskin applicability domain: Indirectly acting haptens, hydrophobic substances and UVCBs	1
Integrated skin sensitization assessment based on OECD methods (III): Adding human data to the assessment_suppl1	1
Perspectives on the evaluation and adoption of complex in vitro models in drug development: Workshop with the FDA and the pharmaceutical industry (IQ MPS Affiliate)	1
Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation_suppl1	1
Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis	1
Guidance for Good In Vitro Reporting Standards (GIVReSt) – A draft for stakeholder discussion and background documentation	1
Phase-out planning for animal experimentation: A definition, an argument, and seven action points	1
3 Days for 3Rs 2023: Refinement, reduction, replacement	1
International conference and workshop on “New Approach Methodologies (NAMs) in Pharmacology and Toxicology”	1
Fish Count, Too – The Animal Toll of REACH Aquatic Toxicity Tests_suppl2	1
The turning point: April 2025 marks historic shift in US animal testing policy	1
Integrated skin sensitization assessment based on OECD methods (III): Adding human data to the assessment_suppl3	1
Review on organ-on-chips for medicines safety assessment: A European regulatory perspective_suppl1	1
Ways to broaden the awareness, consideration and adoption of new approach methodologies (NAMs)	1
Material-mediated pyrogens in medical devices: Myth or reality?_suppl1	1
Impact of gut permeability on estimation of oral bioavailability for chemicals in commerce and the environment	1
A sensitive cell-based assay for testing potency of Botulinum neurotoxin type A_suppl	1
The benefits of validation of methods for toxicity testing outweigh its costs	1
Novel prediction models for genotoxicity based on biomarker genes in human HepaRGTM cells_suppl1	1
Integrated skin sensitization assessment based on OECD methods (III): Adding human data to the assessment_suppl2	1
Amplifying the impact of kidney microphysiological systems: predicting renal drug clearance using mechanistic modelling based on reconstructed drug secretion_suppl	1
Predictive alternative methods for assessing biocompatibility of dental materials: A NIST-NIDCR workshop report	1
Integrated skin sensitization assessment based on OECD methods (II): Hazard and potency by combining kinetic peptide reactivity and the “2 out of 3” Defined Approach_suppl3	1
The impact of biostatistics on hazard characterization using in vitro developmental neurotoxicity assays	1
What does “success” look like in compliance check decisions by the European Chemicals Agency? The curious cases of accepted read-across adaptations_suppl1	0
The importance of variations in in vitro dosimetry to support risk assessment of inhaled toxicants_suppl3	0
Improved identification of human hepatotoxic potential by summary variables of gene expression_suppl	0
New approach methodologies (NAMs): The strategic vision in science	0
Dog as the experimental model: Laboratory uses of dogs in the United States	0
Application of high-throughput transcriptomics for mechanism-based biological read-across of short-chain carboxylic acid analogues of valproic acid_suppl2	0
The GARD™skin assay: Investigation of the applicability domain for metals_suppl	0
Off to a good start? Review of the predictivity of reactivity methods modelling the molecular initiating event of skin sensitization_suppl1	0
Novel prediction models for genotoxicity based on biomarker genes in human HepaRGTM cells	0
No more secrets: Enhancing transparency in animal research	0
60 Years of the 3Rs symposium: Lessons learned and the road ahead	0
A human osteoarthritis mimicking goat cartilage explant-based disease model for drug screening	0
Systematic analysis of read-across adaptations in testing proposal evaluations by the European Chemicals Agency_suppl4	0
Systematic analysis of read-across adaptations in testing proposal evaluations by the European Chemicals Agency_suppl1	0
Integrated skin sensitization assessment based on OECD methods (II): Hazard and potency by combining kinetic peptide reactivity and the “2 out of 3” Defined Approach	0
Challenges and opportunities for validation of AI-based new approach methods	0
Possible applications of new approach methodologies (NAMs) in ecotoxicology	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_Suppl6	0
Workshop report on the evaluation of the updated and expanded carcinogen database to support derivation of threshold of toxicological concern values for DNA reactive carcinogens	0
InViTe2 Retreat: Advancing new approach methodologies in chemical safety testing and drug discovery	0
What does “success” look like in compliance check decisions by the European Chemicals Agency? The curious cases of accepted read-across adaptations_suppl5	0
Mapping physiology: A systems biology approach for the development of alternative methods in toxicology_suppl	0
Organoid intelligence (OI) – The ultimate functionality of a brain microphysiological system	0
An Alternative Safety Profiling Algorithm (ASPA) to transform next generation risk assessment into a structured and transparent process	0
Pollutant exposure and myocardial injury: Protocol and progress report for a toxicological systematic mapping review_Supplement	0
Identifying candidate reference chemicals for in vitro testing of the retinoid pathway for predictive developmental toxicity_suppl2	0
Determination of U-SENS™ borderline range thresholds to address uncertainty for skin sensitization assessment in a regulatory context_suppl1	0
Performance of the DASF compared to other combinations of OECD NAMs for eye hazard identification of surfactants	0
Material-mediated pyrogens in medical devices: Myth or reality?	0
Comparison of structural equation modeling and Bayesian network for de novo construction of a quantitative adverse outcome pathway network	0
A proof-of-concept rat toxicity study highlights the potential utility and challenges of virtual control groups_suppl	0
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl4	0
Considerations from the pharmaceutical industry (IQ MPS affiliate) workshop on animal microphysiological systems and 3Rs in drug development	0
Development of physiologically based gut absorption model for probabilistic prediction of environmental chemical bioavailability	0
Comparison of pyrogen assays by testing products exhibiting low endotoxin recovery_suppl	0
Cardiotoxicity of chemicals: Current regulatory guidelines, knowledge gaps, and needs	0
The road to virtual control groups and the importance of proper body-weight selection	0
Protectiveness of NAM-based hazard assessment – which testing scope is required?_suppl3	0

4.2 million and counting… The animal toll for REACH systemic toxicity studies	0
Trust your gut: Establishing confidence in gastrointestinal models - An overview of the state of the science and contexts of use	0
Practical workshop on replacing fetal bovine serum (FBS) in life science research: From theory into practice	0
Review on organ-on-chips for medicines safety assessment: A European regulatory perspective_suppl2	0
How to formulate hypotheses and IATA to support grouping and read-across of nanoforms_suppl	0
Application of Immunocompetent Microphysiological Systems in Drug Development: Current Perspective and Recommendations	0
A human iPSC-based in vitro neural network formation assay to investigate neurodevelopmental toxicity of pesticides_suppl1	0
Animal pilot studies should not be used to estimate sample size if effect size and population variance are unknown	0
Leveraging biomarkers and translational medicine for preclinical safety - Lessons for advancing the validation of alternatives to animal testing	0
Evaluation of a high-throughput in-vitro-to-in-vivo extrapolation (IVIVE) workflow for the prioritization of potential developmental toxicity of chemicals	0
Zebrafish embryo neonicotinoid developmental neurotoxicity in the FET test and behavioral assays	0
The future of Parkinson’s disease research: A new paradigm of human-specific investigation is necessary… and possible	0
Intestinal in vitro transport assay combined with physiologically based kinetic modeling as a tool to predict bile acid levels in vivo_suppl2	0
Development of an Evidence-Based Risk Assessment Framework	0
Impact of in vitro experimental variation in kinetic parameters on physiologically based kinetic (PBK) model simulations	0
Validation of the monocyte activation test with three therapeutic monoclonal antibodies_suppl	0
The use of categorical regression in evidence integration_suppl	0
National workshop on alternatives to higher animals in toxicology and biomedical science	0
Applications of microphysiological systems to disease models in the biopharmaceutical industry: Opportunities and challenges	0
Revolutionizing developmental neurotoxicity testing – a journey from animal models to advanced in vitro systems	0
How evidence-based methodologies can help identify and reduce uncertainty in chemical risk assessment	0
Systematic analysis of read-across adaptations in testing proposal evaluations by the European Chemicals Agency_suppl3	0
A microfluidic thyroid-liver platform to assess chemical safety in humans	0
In vitro-based prediction of human plasma concentrations of food-related compounds	0
Development of a Monoclonal Antibody Sandwich ELISA for the Determination of Antigen Content and Quality in Human and Animal Tetanus Vaccines	0
Application of microphysiological systems for nonclinical evaluation of cell therapies	0
The importance of variations in in vitro dosimetry to support risk assessment of inhaled toxicants_suppl1	0
Erratum to National Workshop on Alternatives to Higher Animals in Toxicology and Biomedical Science	0
Argentina's progress towards adopting new alternative methods in safety testing: The role of the Laboratorio de Métodos Alternativos	0
Fish count, too – The animal toll of REACH aquatic toxicity tests	0
EU roadmap for phasing out animal testing for chemical safety assessments: Recommendations from a multi-stakeholder roundtable	0
Have the non-technical summaries of animal experiments in Europe improved? An update	0
Protectiveness of NAM-based hazard assessment – which testing scope is required?_suppl2	0
KoCVAM-led validation of KeraSkin™ Phototoxicity Assay for inclusion in OECD TG 498	0
Third Virtual Summer School 3Rs for ONE Science: Alternative methods: From complexity to predictivity	0
Non-animal models: Complexity for interactions…Connecting science	0
A proof-of-concept rat toxicity study highlights the potential utility and challenges of virtual control groups	0
Integrated skin sensitization assessment based on OECD methods (III): Adding human data to the assessment	0
Application of high-throughput transcriptomics for mechanism-based biological read-across of short-chain carboxylic acid analogues of valproic acid	0
AOPs to connect food additives’ effects on gut microbiota to health outcomes	0
Chemical selection for the Thyroid Validation Study coordinated by EURL ECVAM and involving EU-NETVAL laboratories_suppl2	0
What does “success” look like in compliance check decisions by the European Chemicals Agency? The curious cases of accepted read-across adaptations_suppl2	0
Estimation of bioavailable concentration of endogenously formed N-nitrosamines by physiologically based kinetic modelling_suppl	0
Software tools for systematic review literature screening and data extraction: Qualitative user experiences from succinct formal tests	0
A novel coculture system for assessing respiratory sensitizing potential by IL-4 in T cells_suppl	0
Determining a point of departure for skin sensitization potency and quantitative risk assessment of fragrance ingredients using the GARDskin dose-response assay_suppl2	0
In vitro model of neurotrauma using the chick embryo to test regenerative bioimplantation	0
Statistical derivation of cut-off values for in vitro assays	0
Beyond pharmaceuticals: Fit-for-purpose new approach methodologies for environmental cardiotoxicity testing	0
Cross-industrial applications of organotypic models	0
In vitro models of the canine digestive tract as an alternative to in vivo assays: Advances and current challenges	0
Sensitivity analysis and quality indicators for an in vitro oral irritation assay_suppl	0
In vitro evaluation of the carcinogenic potential of perfluorinated chemicals	0
A survey to assess animal methods bias in scientific publishing_suppl5	0
The Implementation Moonshot Project for Alternative Chemical Testing (IMPACT) toward a Human Exposome Project	0
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl5	0
Predictive performance of next generation human physiologically based kinetic (PBK) model predictions based on in vitro and in silico input data_suppl1	0
Comparative evaluation of rat and human in vitro assays for evaluation of thyroid toxicity	0
Challenges integrating skin sensitization data for assessment of difficult to test substances_suppl	0
Assessing proarrhythmic potential of environmental chemicals using a high throughput in vitro-in silico model with human induced pluripotent stem cell-derived cardiomyocytes	0
Development of an Evidence-Based Risk Assessment Framework	0
3D bioprinting of human skin and squamous cell tumors (SCCs) as advanced models for precision medicine (BIOSQIN)	0
Characterization of the C17.2 cell line as testing system for endocrine disruption-induced developmental neurotoxicity	0
State of the science on assessing developmental neurotoxicity using new approach methods	0
Statistical derivation of cut-off values for in vitro assays_suppl	0
Biology-inspired dynamic microphysiological system approaches to revolutionize basic research, healthcare and animal welfare_suppl	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_Suppl5	0
A call for a Human Exposome Project	0
Review on organ-on-chips for medicines safety assessment: A European regulatory perspective	0
Pollutant exposure and myocardial injury: Protocol and progress report for a toxicological systematic mapping review	0
A survey to assess animal methods bias in scientific publishing_suppl2	0
The probable future of toxicology - probabilistic risk assessment	0
Impact of gut permeability on estimation of oral bioavailability for chemicals in commerce and the environment_suppl1	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_suppl3	0
On the usefulness of animals as a model system (part I): Overview of criteria and focus on robustness	0
Integrated skin sensitization assessment based on OECD methods (I): Deriving a point of departure for risk assessment_suppl1	0
Performance of the DASF compared to other combinations of OECD NAMs for eye hazard identification of surfactants_suppl	0
Evaluation of a high-throughput in-vitro-to-in-vivo extrapolation (IVIVE) workflow for the prioritization of potential developmental toxicity of chemicals_suppl2	0
Application of evidence-based methods to construct mechanism-driven chemical assessment frameworks	0
A rodent thyroid-liver chip to capture thyroid toxicity on organ functional level_suppl	0
Comparing translational success rates across medical research fields - A combined analysis of literature and clinical trial data	0
A human iPSC-based in vitro neural network formation assay to investigate neurodevelopmental toxicity of pesticides	0
Opportunities and challenges for human microphysiological systems in drug development	0
Evaluation of a high-throughput in-vitro-to-in-vivo extrapolation (IVIVE) workflow for the prioritization of potential developmental toxicity of chemicals_suppl1	0
Beyond Animal Testing Index: Benchmarking tool for a world beyond animal testing_suppl2	0
Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis_suppl2	0
Identifying candidate reference chemicals for in vitro testing of the retinoid pathway for predictive developmental toxicity	0
ToxAIcology - The evolving role of artificial intelligence in advancing toxicology and modernizing regulatory science	0
Trends in the use of animals and non-animal methods over the last 20 years	0
4.2 million and counting… The animal toll for REACH systemic toxicity studies_suppl	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_suppl2	0
Dog as the experimental model: Laboratory uses of dogs in the United States_suppl	0
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl6	0
The effect of surfactants and film-forming polymers on pulmonary surfactant function measured in vitro is dose rate dependent	0
Making safety decisions for a sunscreen active ingredient using next-generation risk assessment: Benzophenone-4 case study_suppl2	0