Altex-Alternatives to Animal Experimentation

Article	Citations
Probabilistic risk assessment – the keystone for the future of toxicology	40
NAM-supported read-across: From case studies to regulatory guidance in safety assessment	22
Perspectives on the evaluation and adoption of complex in vitro models in drug development: Workshop with the FDA and the pharmaceutical industry (IQ MPS Affiliate)	21
Next-generation risk assessment of chemicals – Rolling out a human-centric testing strategy to drive 3R implementation: The RISK-HUNT3R project perspective	17
Guidance document on Good Cell and Tissue Culture Practice 2.0 (GCCP 2.0)	16
Pyrogen testing revisited on occasion of the 25th anniversary of the whole blood monocyte activation test	13
How a GIVIMP certification program can increase confidence in in vitro methods	11
ToxAIcology - The evolving role of artificial intelligence in advancing toxicology and modernizing regulatory science	11
Organoid intelligence (OI) – The ultimate functionality of a brain microphysiological system	11
Ready for regulatory use: NAMs and NGRA for chemical safety assurance	9
On the usefulness of animals as a model system (part I): Overview of criteria and focus on robustness	9
The state of the scientific revolution in toxicology	9
Reply to comment “Animal welfare and ethics in the collection of fetal blood for the production of fetal bovine serum”	9
Evaluation of an in vitro three-dimensional HepaRG spheroid model for genotoxicity testing using the high-throughput CometChip platform	8
Characteristics to consider when selecting a positive control material for an in vitro assay	8
A strategy towards the generation of testable adverse outcome pathways for nanomaterials	8
A call for a Human Exposome Project	8
Predictive performance of next generation human physiologically based kinetic (PBK) model predictions based on in vitro and in silico input data	7
On the usefulness of animals as a model system (part II): Considering benefits within distinct use domains	7
A new approach methodology (NAM) for the prediction of (nor)ibogaine-induced cardiotoxicity in humans	7
Retrospective analysis of dermal absorption triple pack data	6
Implementing in vitro bioactivity data to modernize priority setting of chemical inventories	6
Consensus statement from the European Network of 3R Centres (EU3Rnet)	6
New approach methods supporting read-across: Two neurotoxicity AOP-based IATA case studies	6
U.S. Federal Agency interests and key considerations for new approach methodologies for nanomaterials	5

COVID-19 through Adverse Outcome Pathways: Building networks to better understand the disease – 3rd CIAO AOP Design Workshop	5
Replacing fetal bovine serum (FBS) in research and testing	5
The future of Parkinson’s disease research: A new paradigm of human-specific investigation is necessary… and possible	5
Report of the 1st and 2nd Mystery of Reactive Oxygen Species Conferences	5
Limitations of animal epilepsy research models: Can epileptic human tissue provide translational benefit?	5
A human iPSC-based in vitro neural network formation assay to investigate neurodevelopmental toxicity of pesticides	5
Integrated skin sensitization assessment based on OECD methods (III): Adding human data to the assessment	5
Educational use of animals in Europe indicates a reluctance to implement alternatives	4
Applying a next generation risk assessment framework for skin sensitisation to inconsistent new approach methodology information	4
Academic application of Good Cell Culture Practice for induced pluripotent stem cells	4
Using the monocyte activation test as a stand-alone release test for medical devices	4
Leveraging microphysiological systems to address challenges encountered during development of oligonucleotide therapeutics	4
Mind the gaps: Prioritizing activities to meet regulatory needs for acute systemic lethality	4
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells	4
Engagement of scientists with the public and policymakers to promote alternative methods	4
How to formulate hypotheses and IATA to support grouping and read-across of nanoforms	4
A microfluidic thyroid-liver platform to assess chemical safety in humans	4
4.2 million and counting… The animal toll for REACH systemic toxicity studies	4
Continuing animal tests on cosmetic ingredients for REACH in the EU	4
Development of a network of carcinogenicity adverse outcome pathways and its employment as an evidence framework for safety assessment	4
Proceedings of a workshop to address animal methods bias in scientific publishing	4
Integrated skin sensitization assessment based on OECD methods (II): Hazard and potency by combining kinetic peptide reactivity and the “2 out of 3” Defined Approach	4
A high-throughput and highly automated genotoxicity screening assay	4
Beyond chemicals: Opportunities and challenges of integrating non-chemical stressors in adverse outcome pathways	3
Improving the quality of toxicology and environmental health systematic reviews: What journal editors can do	3
A rodent thyroid-liver chip to capture thyroid toxicity on organ functional level	3
REACH out-numbered! The future of REACH and animal numbers	3
Animal welfare and ethics in the collection of fetal blood for the production of fetal bovine serum	3
Grouping of UVCB substances with dose-response transcriptomics data from human cell-based assays	3
A triangular approach for the validation of new approach methods for skin sensitization	3
Potential of concentration-response data to broaden regulatory application of in vitro test guidelines	3
Application of evidence-based methods to construct mechanism-driven chemical assessment frameworks	3
A survey to assess animal methods bias in scientific publishing	3
Applying evidence-based methods to the development and use of adverse outcome pathways	3
COVID-19 – prime time for microphysiological systems, as illustrated for the brain	3
Acceptance criteria for new approach methods in toxicology and human health-relevant life science research – part I	3
Amplifying the impact of kidney microphysiological systems: predicting renal drug clearance using mechanistic modelling based on reconstructed drug secretion	3
Beyond pharmaceuticals: Fit-for-purpose new approach methodologies for environmental cardiotoxicity testing	3
Phase-out planning for animal experimentation: A definition, an argument, and seven action points	2
Development of an Evidence-Based Risk Assessment Framework	2
An alternative in vivo model to evaluate pluripotency of patient-specific iPSCs	2
Integrated skin sensitization assessment based on OECD methods (I): Deriving a point of departure for risk assessment	2
Novel prediction models for genotoxicity based on biomarker genes in human HepaRGTM cells	2
Open-source human skin model with an in vivo-like barrier for drug testing	2
Incorporating new approach methodologies into regulatory nonclinical pharmaceutical safety assessment	2
Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation	2
Development of an Evidence-Based Risk Assessment Framework	2
Dog as the experimental model: Laboratory uses of dogs in the United States	2
The Monographs Programme of the International Agency for Research on Cancer	2
Technical framework for enabling high-quality measurements in new approach methodologies (NAMs)	2

Animal metrics: Tracking contributions of new approach methods to reduced animal use	2
A novel coculture system for assessing respiratory sensitizing potential by IL-4 in T cells	2
Identifying candidate reference chemicals for in vitro testing of the retinoid pathway for predictive developmental toxicity	2
Exploration of the GARDskin applicability domain: Indirectly acting haptens, hydrophobic substances and UVCBs	2
Understanding COVID-19 through adverse outcome pathways – 2nd CIAO AOP Design Workshop	2
Comparing translational success rates across medical research fields - A combined analysis of literature and clinical trial data	2
The probable future of toxicology - probabilistic risk assessment	2
Application of Immunocompetent Microphysiological Systems in Drug Development: Current Perspective and Recommendations	2
Alternative methods go green! Green toxicology as a sustainable approach for assessing chemical safety and designing safer chemicals	2
Off to a good start? Review of the predictivity of reactivity methods modelling the molecular initiating event of skin sensitization	2
SpheraCosmolife: a new tool for the risk assessment of cosmetic products	2
Applications of microphysiological systems to disease models in the biopharmaceutical industry: Opportunities and challenges	2
Impact of in vitro experimental variation in kinetic parameters on physiologically based kinetic (PBK) model simulations	2
Elements and development processes for test methods in toxicology and human health-relevant life science research	2
Application of high-throughput transcriptomics for mechanism-based biological read-across of short-chain carboxylic acid analogues of valproic acid	2
The benefits of validation of methods for toxicity testing outweigh its costs	1
Workshop report on the evaluation of the updated and expanded carcinogen database to support derivation of threshold of toxicological concern values for DNA reactive carcinogens	1
The nearest neighbor nuclei method to objectify analysis of pertussis toxin-induced clustering	1
Comparison of structural equation modeling and Bayesian network for de novo construction of a quantitative adverse outcome pathway network	1
Development of physiologically based gut absorption model for probabilistic prediction of environmental chemical bioavailability	1
The use of categorical regression in evidence integration	1
Lung tumor microphysiological system with 3D endothelium to evaluate modulators of T-cell migration	1
Report on 2021 International Workshop for Non-animal Approaches in the Food Sector (Japan): Current status and avenues for further research	1
Exploratory analysis of the application of animal reduction approaches in proteomics: How much is enough?	1
Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity	1
The GARD™skin assay: Investigation of the applicability domain for metals	1
How evidence-based methodologies can help identify and reduce uncertainty in chemical risk assessment	1
Pollutant exposure and myocardial injury: Protocol and progress report for a toxicological systematic mapping review	1
Zebrafish embryo neonicotinoid developmental neurotoxicity in the FET test and behavioral assays	1
Systematic review in evidence-based risk assessment	1
Toward implementing virtual control groups in nonclinical safety studies	1
EFSA’s framework for evidence-based scientific assessments: A case study on uncertainty analysis	1
Integrated skin sensitization assessment based on OECD methods (I): Deriving a point of departure for risk assessment_suppl4	1
Microphysiological endothelial models to characterize subcutaneous drug absorption	1
Predictive alternative methods for assessing biocompatibility of dental materials: A NIST-NIDCR workshop report	1
In vitro-based prediction of human plasma concentrations of food-related compounds	1
Second Virtual Summer School: Alternative methods in science: Towards model complexity	1
Report of the 3rd and 4th Mystery of Reactive Oxygen Species Conference	1
Predictive performance of next generation human physiologically based kinetic (PBK) model predictions based on in vitro and in silico input data_suppl2	1
Practical workshop on replacing fetal bovine serum (FBS) in life science research: From theory into practice	1
Identifying, naming and documenting of test and tool compound stocks	1
Guidelines for FAIR sharing of preclinical safety and off-target pharmacology data	1
An in vitro air-liquid interface inhalation platform for petroleum substances and constituents	1
In vitro models of the canine digestive tract as an alternative to in vivo assays: Advances and current challenges	1
Russell and Burch’s 3Rs then and now: The case of Switzerland	1
Integrated skin sensitization assessment based on OECD methods (II): Hazard and potency by combining kinetic peptide reactivity and the “2 out of 3” Defined Approach_suppl4	1
Sensitization potential of medical devices detected by in vitro and in vivo methods	1
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_Suppl7	0
Animal research ethics as interaction of research ethics, animal ethics, and (animal protection) law	0
Assessing proarrhythmic potential of environmental chemicals using a high throughput in vitro-in silico model with human induced pluripotent stem cell-derived cardiomyocytes_suppl1	0
A human iPSC-based in vitro neural network formation assay to investigate neurodevelopmental toxicity of pesticides_suppl1	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_Suppl4	0
COVID-19 through Adverse Outcome Pathways: Building networks to better understand the disease – 3rd CIAO AOP Design Workshop_suppl	0
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl6	0
The importance of variations in in vitro dosimetry to support risk assessment of inhaled toxicants_suppl2	0
Understanding COVID-19 through adverse outcome pathways – 2nd CIAO AOP Design Workshop_suppl	0
A human iPSC-based in vitro neural network formation assay to investigate neurodevelopmental toxicity of pesticides_suppl2	0
Sensitivity analysis and quality indicators for an in vitro oral irritation assay_suppl	0
A new approach methodology (NAM) for the prediction of (nor)ibogaine-induced cardiotoxicity in humans_suppl	0
The use of categorical regression in evidence integration_suppl	0
Comparison of pyrogen assays by testing products exhibiting low endotoxin recovery	0
An in vitro air-liquid interface inhalation platform for petroleum substances and constituents_suppl	0
Impact of in vitro experimental variation in kinetic parameters on physiologically based kinetic (PBK) model simulations_suppl1	0
4.2 million and counting… The animal toll for REACH systemic toxicity studies_suppl	0
	0
Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis	0
Incorporating new approach methodologies into regulatory nonclinical pharmaceutical safety assessment_suppl	0
Open-source human skin model with an in vivo-like barrier for drug testing_suppl	0
Validation of the monocyte activation test with three therapeutic monoclonal antibodies	0
Comparing translational success rates across medical research fields - A combined analysis of literature and clinical trial data_suppl1	0
The 3Rs in animal welfare bodies at Swedish universities – knowledge, attitudes, implementation	0
Sensitivity analysis and quality indicators for an in vitro oral irritation assay	0
Software tools for systematic review literature screening and data extraction: Qualitative user experiences from succinct formal tests	0
Evaluation of an in vitro three-dimensional HepaRG spheroid model for genotoxicity testing using the high-throughput CometChip platform_suppl	0
The validation of regulatory test methods – Conceptual, ethical, and philosophical foundations	0
Integrated skin sensitization assessment based on OECD methods (III): Adding human data to the assessment_suppl2	0
A sensitive cell-based assay for testing potency of Botulinum neurotoxin type A	0
Implementing in vitro bioactivity data to modernize priority setting of chemical inventories_suppl2	0
Characterization and optimization of variability in a human colonic epithelium culture model	0
Pollutant exposure and myocardial injury: Protocol and progress report for a toxicological systematic mapping review_Supplement	0

Development of a defined approach for eye hazard identification of solid chemicals according to the three UN GHS categories	0
Exploratory analysis of the application of animal reduction approaches in proteomics: How much is enough?_suppl	0
Proceedings of a workshop to address animal methods bias in scientific publishing_suppl	0
Microphysiological endothelial models to characterize subcutaneous drug absorption_suppl	0
Intestinal in vitro transport assay combined with physiologically based kinetic modeling as a tool to predict bile acid levels in vivo_suppl1	0
The road to virtual control groups and the importance of proper body-weight selection_suppl	0
Integrated skin sensitization assessment based on OECD methods (I): Deriving a point of departure for risk assessment_suppl1	0
Development of an Evidence-Based Risk Assessment Framework_suppl	0
Linking nanomaterial-induced mitochondrial dysfunction to existing adverse outcome pathways for chemicals_suppl1	0
Protectiveness of NAM-based hazard assessment – which testing scope is required?_suppl3	0
Beyond Animal Testing Index: Benchmarking tool for a world beyond animal testing_suppl1	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_Suppl5	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_Suppl1	0
A novel coculture system for assessing respiratory sensitizing potential by IL-4 in T cells_suppl	0
Virtual class on alternative methods: Ethics and science	0
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl2	0
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl3	0
The importance of variations in in vitro dosimetry to support risk assessment of inhaled toxicants_suppl3	0
Novel prediction models for genotoxicity based on biomarker genes in human HepaRGTM cells_suppl1	0
Have the non-technical summaries of animal experiments in Europe improved? An update	0
A survey to assess animal methods bias in scientific publishing_suppl1	0
Potential value of animal microphysiological systems	0
Application of computational methods in replacement – an IPAM webinar	0
Grouping of UVCB substances with dose-response transcriptomics data from human cell-based assays_suppl	0
Enabling a shift towards a human-relevant and predictive paradigm for biomedical research and drug discovery in India	0
Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells_suppl1	0
A human osteoarthritis mimicking goat cartilage explant-based disease model for drug screening	0
Linking nanomaterial-induced mitochondrial dysfunction to existing adverse outcome pathways for chemicals_suppl2	0
Academic application of Good Cell Culture Practice for induced pluripotent stem cells_suppl	0
The 3Rs in animal welfare bodies at Swedish universities – knowledge, attitudes, implementation_suppl	0
Beyond Animal Testing Index: Benchmarking tool for a world beyond animal testing_suppl3	0
Characterization of the C17.2 cell line as testing system for endocrine disruption-induced developmental neurotoxicity	0
Impact of gut permeability on estimation of oral bioavailability for chemicals in commerce and the environment_suppl1	0
Revolutionizing developmental neurotoxicity testing – a journey from animal models to advanced in vitro systems	0
An alternative in vivo model to evaluate pluripotency of patient-specific iPSCs_suppl	0
Off to a good start? Review of the predictivity of reactivity methods modelling the molecular initiating event of skin sensitization_suppl2	0
Predicting Acute Oral Toxicity Using AcutoX: An Animal Product-Free and Metabolically Relevant Human Cell-Based Test	0
A new gold standard: Paradigm shift in research on human diseases and therapy developments	0
National workshop on alternatives to higher animals in toxicology and biomedical science	0
Integrated skin sensitization assessment based on OECD methods (III): Adding human data to the assessment_suppl3	0
Trends in the use of animals and non-animal methods over the last 20 years	0
Qualitative and quantitative concentration-response modelling of gene co-expression networks to unlock hepatotoxic mechanisms for next generation chemical safety assessment	0
A cell-based alternative to the mouse potency assay for pharmaceutical type E botulinum antitoxins	0
Development of a Monoclonal Antibody Sandwich ELISA for the Determination of Antigen Content and Quality in Human and Animal Tetanus Vaccines	0
Characterization of the C17.2 cell line as testing system for endocrine disruption-induced developmental neurotoxicity_suppl	0
Indian Society for Alternatives to Animal Experiments: Sixth annual meeting and international conference	0
	0
A sensitive cell-based assay for testing potency of Botulinum neurotoxin type A_suppl	0
Comparison of pyrogen assays by testing products exhibiting low endotoxin recovery_suppl	0
Beyond Animal Testing Index: Benchmarking tool for a world beyond animal testing_suppl4	0
Application of high-throughput transcriptomics for mechanism-based biological read-across of short-chain carboxylic acid analogues of valproic acid_suppl2	0
Implementing in vitro bioactivity data to modernize priority setting of chemical inventories_suppl1	0
Protectiveness of NAM-based hazard assessment – which testing scope is required?	0
Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis_suppl2	0
Potential of concentration-response data to broaden regulatory application of in vitro test guidelines_suppl	0
Risk-based prioritization of PFAS using phenotypic and transcriptomic data from human induced pluripotent stem cell-derived hepatocytes and cardiomyocytes_Suppl8	0
Replacement, reduction, refinement: 3 days for 3Rs	0
Alternative methods and citizen science	0
A survey to assess animal methods bias in scientific publishing_suppl5	0
Data-driven derivation of an adverse outcome pathway linking vascular endothelial growth factor receptor (VEGFR), endocrine disruption, and atherosclerosis_suppl1	0
Development of physiologically-based gut absorption model for probabilistic prediction of environmental chemical bioavailability_suppl	0
No more secrets: Enhancing transparency in animal research	0
The GARD™skin assay: Investigation of the applicability domain for metals_suppl	0
Predictive performance of next generation human physiologically based kinetic (PBK) model predictions based on in vitro and in silico input data_suppl1	0
Impact of in vitro experimental variation in kinetic parameters on physiologically based kinetic (PBK) model simulations_suppl2	0
VitroScreen Seminar - Substance-based medical device qualification and classification: Pre-clinical approaches to mechanism of action	0
Photomotor response data analysis approach to assess chemical neurotoxicity with the zebrafish embryo_suppl	0
SpheraCosmolife: a new tool for the risk assessment of cosmetic products_suppl	0
Summer school on innovative approaches in science	0
Photomotor response data analysis approach to assess chemical neurotoxicity with the zebrafish embryo	0
3D bioprinting of human skin and squamous cell tumors (SCCs) as advanced models for precision medicine (BIOSQIN)	0
Open letter: Selection of a new Executive Director of the European Chemicals Agency (ECHA) provides an opportunity for the EU to lead in the field of chemicals management and implementation of innovat	0
Systematic review in evidence-based risk assessment_suppl	0
The impact of biostatistics on hazard characterization using in vitro developmental neurotoxicity assays_suppl	0
Challenges integrating skin sensitization data for assessment of difficult to test substances_suppl	0
A proof-of-concept rat toxicity study highlights the potential utility and challenges of virtual control groups_suppl	0
Protectiveness of NAM-based hazard assessment – which testing scope is required?_suppl1	0
Amplifying the impact of kidney microphysiological systems: predicting renal drug clearance using mechanistic modelling based on reconstructed drug secretion_suppl	0
Leveraging biomarkers and translational medicine for preclinical safety - Lessons for advancing the validation of alternatives to animal testing	0
A survey to assess animal methods bias in scientific publishing_suppl3	0
A proof-of-concept rat toxicity study highlights the potential utility and challenges of virtual control groups	0
A strategy towards the generation of testable adverse outcome pathways for nanomaterials_suppl	0
Micro-replace systems	0
Alternative Methods in Science: Towards Fluidic Systems	0
E-validation – Unleashing AI for validation	0
Application of high-throughput transcriptomics for mechanism-based biological read-across of short-chain carboxylic acid analogues of valproic acid_suppl1	0
Novel prediction models for genotoxicity based on biomarker genes in human HepaRGTM cells_suppl2	0
The Implementation Moonshot Project for Alternative Chemical Testing (IMPACT) toward a Human Exposome Project	0
Transition to animal-free science: Phasing out animal experiments, phasing in innovation	0
The road to virtual control groups and the importance of proper body-weight selection	0
Integrated skin sensitization assessment based on OECD methods (II): Hazard and potency by combining kinetic peptide reactivity and the “2 out of 3” Defined Approach_suppl2	0
The importance of variations in in vitro dosimetry to support risk assessment of inhaled toxicants_suppl1	0
Identifying candidate reference chemicals for in vitro testing of the retinoid pathway for predictive developmental toxicity_suppl	0
Beyond Animal Testing Index: Benchmarking tool for a world beyond animal testing_suppl5	0
Beyond Animal Testing Index: Benchmarking tool for a world beyond animal testing_suppl2	0