Biotechnology Journal

Article	Citations
	100
Peptonics: A new family of cell‐protecting surfactants for the recombinant expression of therapeutic proteins in mammalian cell cultures	88
Enhanced anti‐tumor activity of arginine decarboxylase through the incorporation of aromatic amino acids at the multimer‐forming interface	82
	78
A biotechnological approach for the production of new protein bioplastics	64
Antibacterial functionalization of calfskin bioremediation film	38
Development and optimization of a frequency mixing sensor for adjacent samples quantitative detection on a lateral flow assay	37
In vitro and in silico analyses reveal the interaction between LysM receptor‐like kinase3 of Solanum tuberosum and the carbohydrate elicitor Riclin octaose	31
Antibody mimetics: The next generation antibody engineering, a retrospective and prospective analysis	29
Evaluation of a novel purified and characterized alkaline protease from the extremophile Exiguobacterium alkaliphilum VLP1 as a detergent additive	29
Site specific insertion of a transgene into the murine α‐casein (CSN1S1) gene results in the predictable expression of a recombinant protein in milk	25
CRISPR/Cas9‐mediated knockout of a prolyl‐4‐hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection	25
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli	23
	22
Rational design and experimental evaluation of peptide ligands for the purification of adeno‐associated viruses via affinity chromatography	22
Outside Front Cover: (Biotechnology Journal 7/2023)	22
Construction and application of a new CRISPR/Cas12a system in Stenotrophomonas AGS‐1 from aerobic granular sludge	22
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Rsn‐2‐mediated directed foam enrichment of β‐lactamase	21
A 3′‐end capture sequencing method for high‐throughput targeted gene expression profiling	20
Optimising protein detection with fixable custom oligo‐labelled antibodies for single‐cell multi‐omics approaches	20
Editorial Board: Biotechnology Journal 8/2021	19
Outside Front Cover: (Biotechnology Journal 12/2021)	19
A single‐cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing	19
Outside Front Cover: (Biotechnology Journal 5/2021)	19

Outside Front Cover: (Biotechnology Journal 6/2022)	18
Editorial Board: Biotechnology Journal 7/2021	18
Cell fate‐inducing CARs orthogonally control multiple signaling pathways	18
	17
Metabolic regulation and optimization of oxygen supply enhance the 2,3‐butanediol yield of the novel Klebsiella sp. isolate FSoil 024	16
Toehold switch plus signal amplification enables rapid detection	16
Characterization of essential elements for improved episomal expressions in Kluyveromyces marxianus	16
Umbilical cord derived mesenchymal stromal cells in microcarrier based industrial scale culture sustain the immune regulatory functions	15
Outside Front Cover: (Biotechnology Journal 9/2022)	15
Editorial Board: Biotechnology Journal 10/2022	15
Bioinspired scaffolds based on aligned polyurethane nanofibers mimic tendon and ligament fascicles	15
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Medium optimization and subsequent fermentative regulation enabled the scaled‐up production of anti‐tuberculosis drug leads ilamycin‐E1/E2	15
Efficient production of glutathione in Saccharomyces cerevisiae via a synthetic isozyme system	15
Editorial Board: Biotechnology Journal 11/2022	15
Serial enrichment of heteroduplex DNA using a MutS‐magnetic bead system	14
Advancing a rapid, high throughput screening platform for optimization of lentivirus production	14
Redesigning glycolaldehyde synthase for the synthesis of biorefinery feedstock D‐xylulose from C1 compounds	14
Rapid production of multimeric RNA aptamers stabilized by a designed pseudo‐circular structure in E. coli	14
Outside Front Cover: (Biotechnology Journal 8/2023)	14
Outside Front Cover: (Biotechnology Journal 10/2021)	14
	14
Editorial Board: Biotechnology Journal 4/2022	14
Intermediate product control in cascade reaction for one‐pot production of ε‐caprolactone by Escherichia coli	14
Synergistic effects of biological stimuli and flexion induce microcavities promote hypertrophy and inhibit chondrogenesis during in vitro culture of human mesenchymal stem cell aggregates	14
CRISPR/Cas9‐based genome engineering in the filamentous fungus Rhizopus oryzae and its application to L‐lactic acid production	14
Ribozyme‐processed guide RNA enhances virus‐mediated plant genome editing	13
Intracellular transformation and disposal mechanisms of magnetosomes in macrophages and cancer cells	13
Evaluation of the cytotoxic activity of sorafenib‐loaded camel milk casein nanoparticles against hepatocarcinoma cells	13
Quercetin‐loaded platelets as a potential targeted therapy for glioblastoma multiforme cell line U373‐MG	12
Potential of butanol production from Thailand marine macroalgae using Clostridium beijerinckii ATCC 10132‐based ABE fermentation	12
Editorial Board: Biotechnology Journal 3/2021	12
Spinal cord regeneration: A brief overview of the present scenario and a sneak peek into the future	12
Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis	12
Immobilization of transgenic plant cells towards bioprinting for production of a recombinant biodefense agent	12
Outside Front Cover: (Biotechnology Journal 3/2021)	12
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications	11
1H NMR‐based process understanding and biochemical marker identification methodology for monitoring CHO cell culture process during commercial‐scale manufacturing	11
Evaluation of expression systems for recombinant protein production in chicken egg bioreactors	11
Phase separation methods for protein purification: A meta‐analysis of purification performance and cost‐effectiveness	11
Recent advances in applying omic technologies for studying acetic acid bacteria in industrial vinegar production: A comprehensive review	11
Developing nanosize carrier systems for Amphotericin‐B: A review on the biomedical application of nanoparticles for the treatment of leishmaniasis and fungal infections	11
Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP	11
Expression and application of aflatoxin degrading enzyme gene in Pichia pastoris	11
Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation	11
Advancements in scaffold for treating ligament injuries; in vitro evaluation	11
E and M SARS‐CoV‐2 membrane protein expression and enrichment with plant lipid droplets	11
Tri‐enzyme fusion of tryptophan halogenase achieves a concise strategy for coenzyme self‐sufficiency and the continuous halogenation of L‐tryptophan	11
Cytosolic protein delivery using ultrasound‐guided vaporization of perfluorocarbon nano‐droplets	10
In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry	10

Outside Front Cover: (Biotechnology Journal 4/2024)	10
Engineering and transcriptome study of Saccharomyces cerevisiae to produce ginsenoside compound K by glycerol	10
Initial cyclic‐di‐GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival	10
Identification of critical process parameters for expansion of clinical grade human Wharton's jelly‐derived mesenchymal stromal cells in stirred‐tank bioreactors	10
Biological autoluminescence enables effective monitoring of yeast cell electroporation	10
Production of D‐tagatose, bioethanol, and microbial protein from the dairy industry by‐product whey powder using an integrated bioprocess	10
Characterization of the Ubiquitin‐Modified Proteome of Recombinant Chinese Hamster Ovary Cells in Response to Endoplasmic Reticulum Stress	9
Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: Implications for baculovirus expression	9
High fidelity one‐pot DNA assembly using orthogonal serine integrases	9
Combined approach of selective and accelerated cloning for microfluidic chip‐based system increases clone specific productivity	9
The prediction model of nitrogen nutrition in cotton canopy leaves based on hyperspectral visible‐near infrared band feature fusion	9
Improved synthesis of dietary triglycerides by using lipase supported on clay carriers	9
Multifunctional PAMAM Dendrimers Carrying SAHA, 5‐FU, and a Therapeutic Gene for Targeted Co‐Delivery Toward Colorectal Cancer Cells	9
Real‐Time Adaptive Inline Acidification Enhances Continuous pH Control for Viral Inactivation	9
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Enhancing erythromycin production in Saccharopolyspora erythraea through rational engineering and fermentation refinement: A Design‐Build‐Test‐Learn approach	9
A Hybrid Model Simulating Multi‐Stage Continuous Fermentation of Saccharomyces cerevisiae	9
Reducing water uptake into BY‐2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs	9
CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome	9
Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris	9
Engineering Saccharomyces boulardii for Probiotic Supplementation of l‐Ergothioneine	9
Integrated process development: The key to improve Fab production in E. coli	9
Short‐chain alcohols inactivate an immobilized industrial lipase through two different mechanisms	9
Generation of stable suspension producer cell lines for serum‐free lentivirus production	9
Dimethyloxalylglycine, a small molecule, synergistically increases the homing and angiogenic properties of human mesenchymal stromal cells when cultured as 3D spheroids	9
Parallel and large‐scale antitumor investigation using stable chemical gradient and heterotypic three‐dimensional tumor coculture in a multi‐layered microfluidic device	9
Enhanced production of difficult‐to‐express proteins through knocking down rnpA gene expression	8
Studying the impact of cell age on the yeast growth behaviour ofSaccharomyces pastorianusvar.carlsbergensisby magnetic separation	8
Biomedical application of microalgal‐biomaterials hybrid system	8
Liamocin biosynthesis is induced by an autogenous host acid activation in Aureobasidium melanogenum	8
Scaling strategy for cell and gene therapy bioreactors based on turbulent parameters	8
Experimental study of the topological flow transformations in an aerial vortex bioreactor with a floating washer	8
Issue Information: Biotechnology Journal 6/2024	8
Expanding the RNA polymerase biocatalyst solution space for mRNA manufacture	8
Development of an in‐line Raman analytical method for commercial‐scale CHO cell culture process monitoring: Influence of measurement channels and batch number on model performance	8
F‐actin microfilaments affect the LIPUS‐promoted osteogenic differentiation of BMSCs through TRPM7	8
A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single‐cell suspension	8
Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d‐amino acid oxidase	8
Engineering of the start condensation domain with improved N‐decanoyl catalytic activity for daptomycin biosynthesis	8
Establishment of a cell culture platform for human liver organoids and its application for lipid metabolism research	8
Biological potential of polysaccharides extracted from Nostoc colonies for film production –  Physical and biological properties	8
Molecular design of controllable recombinant adeno‐associated virus (AAV) expression systems for enhanced vector production	8
A 3D bioprinted antibacterial hydrogel dressing of gelatin/sodium alginate loaded with ciprofloxacin hydrochloride	8
Truncation of a novel C‐terminal domain of a β‐glucanase improves its thermal stability and specific activity	8
Engineering a 3‐dimensional tissue construct with adipose‐derived stem cells for healing bone defect: An ex vivo study with femur head	8
Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells	8
A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption	8
Live‐cell imaging of genomic loci with Cas9 variants	7
Biotransformation of ethylene glycol to glycolic acid by Yarrowia lipolytica: A route for poly(ethylene terephthalate) (PET) upcycling	7
Depolymerization of the polyester–polyurethane by amidase GatA250 and enhancing the production of 4,4′‐methylenedianiline with cutinase LCC	7
Dialysis rolled scaffold bioreactor allows extended production of monoclonal antibody with reduced media use	7
Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads	7
Novel erythropoietin‐based therapeutic candidates with extra N‐glycan sites that block hematopoiesis but preserve neuroplasticity	7
An engineered microfluidic blood‐brain barrier model to evaluate the anti‐metastatic activity of β‐boswellic acid	7
The role of intermolecular interactions on monoclonal antibody filtration through virus removal membranes	7
Adapting microalgae‐based strategies for sustainable green cities	7
Visualize PIM‐1 Protein Function and Its Interaction With PI3K/Akt/mTOR Pathway Regulated by Its Active Sites Through FRET Biosensors	7
Precision genome editing in plants using gene targeting and prime editing: existing and emerging strategies	7
Customizing amino acid metabolism of Pichia pastoris for recombinant protein production	7
Highly efficient CRISPR‐mediated base editing for the gut Bacteroides spp. with pnCasBS‐CBE	7
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS‐1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle	7
State of the art of bone biomaterials and their interactions with stem cells: Current state and future directions	7
Fluid shear force and hydrostatic pressure jointly promote osteogenic differentiation of BMSCs by activating YAP1 and NFAT2	7
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae	7
Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles	7
Fluorescence‐based screening for engineered aldo‐keto reductase KmAKR with improved catalytic performance and extended substrate scope	7
Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (stinging nettle) leaf extract: Antimicrobial and anticancer activity	7
Targeting metabolic driving and minimization of by‐products synthesis for high‐yield production of D‐pantothenate in Escherichia coli	7
Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria	7
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Subtractive Inhibition Assay Based on PagN‐Specific Monoclonal Antibody for the Detection of Salmonella Using Surface Plasmon Resonance	6
High‐efficiency bioconversion of phytosterol to bisnoralcohol by metabolically engineered Mycobacterium neoaurum in a micro‐emulsion system	6
A high‐fidelity, dual site‐specific integration system in CHO cells by a Bxb1 recombinase	6
Extraction, separation and purification of fatty acid ethyl esters for biodiesel and DHA from Thraustochytrid biomass	6
Aptabinding of tumor necrosis factor‐α (TNFα) inhibits its proinflammatory effects and alleviates islet inflammation	6
Bio‐based polymers containing traditional medicinal fillers for wound healing applications – An evaluation of neoteric development and future perspectives	6
Fabrication of a decellularized liver matrix–based hepatic patch for the repair of CCl4‐induced liver injury	6
Integrated nucleic acid purification technology based on amino‐modified centrifugal microfluidic chip	6
Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology	6
Proteomics Reveals Distinctive Host Cell Protein Expression Patterns in Fed‐Batch and Perfusion Cell Culture Processes	6

Indigenous microalgae strains characterization for a sustainable biodiesel production	6
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An ultrasonic biosample disruptor with two triangular teeth on its radiation face	6
The choice of ultra‐low attachment plates impacts primary human and primary canine hepatocyte spheroid formation, phenotypes, and function	6
Correction to “3D artificial sebocyte glands from inertial focusing effect for facile and flexible analysis of light damage and drug screening”	6
Editorial Board: Biotechnology Journal 12/2021	6
Osteochondral Tissue Engineering: Scaffold Materials, Fabrication Techniques and Applications	6
Enhancement of rAAV titers via inhibition of the interferon signaling cascade in transfected HEK293 suspension cultures	6
Engineering cannabinoid production in Saccharomyces cerevisiae	6
Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered Yarrowia lipolytica	6
Infectious clone of a contemporary Tembusu virus and replicons expressing reporter genes or heterologous antigens from poultry viruses	6
Prediction of CHO cell line stability using expression of DNA repair genes	6
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Development of the IMP biosensor for rapid and stable analysis of IMP concentrations in fermentation broth	6
3D artificial sebocyte glands from inertial focusing effect for facile and flexible analysis of light damage and drug screening	5
Enhancing the accumulation of lipid and docosahexaenoic acid in Schizochytrium sp. by co‐overexpression of phosphopantetheinyl transferase and ω‐3 fatty acid desaturase	5
Microalgae‐made human vaccines and therapeutics: A decade of advances	5
High‐activity recombinant human carboxypeptidase B expression in Pichia pastoris through rational protein engineering and enhancing secretion from the Golgi apparatus to the plasma membrane	5
The role of the CRISPR‐Cas system in cancer drug development: Mechanisms of action and therapy	5
Nanomaterials for Biomedical Applications	5
Feasibility and performance of cross‐clone Raman calibration models in CHO cultivation	5
The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism	5
A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli	5
Degenerative disease‐on‐a‐chip: Developing microfluidic models for rapid availability of newer therapies	5
A Novel and Simplified Anion Exchange Flow‐Through Polishing Approach for the Separation of Full From Empty Adeno‐Associated Virus Capsids	5
Sodium formate redirects carbon flux and enhances heterologous mevalonate production in Methylobacterium extorquens AM1	5
Tuning mobile phase properties to improve empty and full particle separation in adeno‐associated virus productions by anion exchange chromatography	5
L‐malic acid production from xylose by engineered Saccharomyces cerevisiae	5
Prime editing‐mediated correction of the leptin receptor in muscle cells of db/db mice	5
Accelerated generation of gene‐engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9	5
Outside Front Cover: (Biotechnology Journal 9/2023)	5
Enhanced L‐serine production by Corynebacterium glutamicum based on novel insights into L‐serine exporters	5
Direct capture and selective elution of a secreted polyglutamate‐tagged nanobody using bare magnetic nanoparticles	5
Metabolic engineering of Aspergillus niger for accelerated malic acid biosynthesis by improving NADPH availability	5
Lyophilization of premixed COVID‐19 diagnostic RT‐qPCR reactions enables stable long‐term storage at elevated temperature	5
25SrRNA Methyltransferase CgBMT5 From Candida glycerinogenes Improves Tolerance and Fermentation Performance of Saccharomyces cerevisiae and Yarrowia lipolytica From Undetoxified	5
Genome‐Scale Metabolic Modeling of Halomonas elongata 153B Explains Polyhydroxyalkanoate and Ectoine Biosynthesis in Hypersaline Environments	5
Editorial Board: Biotechnology Journal 5/2022	5
Assessing the predictive capabilities of design heuristics and coarse‐grain simulation toward understanding and optimizing site‐specific covalent immobilization of β‐lactamase	5
Protein loss during membrane processes in biopharmaceutical manufacturing	5
Innovative translational platforms for rapid developing clinical vaccines against COVID‐19 and other infectious disease	5
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Inducible transgene expression in CHO cells using an artificial transcriptional activator with estrogen‐binding domain	5
Development of an engineered Bacillus subtilis strain for antibiotic‐free sucrose isomerase production	5
In vitro acne disease model from inertial focusing effect for studying the interactions between sebocyte glands and macrophages	5
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Metabolic Engineering of Saccharomyces cerevisiae for Fermentative Production of Heme	5
Concentration‐Dependent Effects of MXene Nanocomposite‐Loaded Carboxymethyl Cellulose on Wound Healing	5
Encapsulation of Pseudomonas aeruginosa elastase inside the P22 virus‐like particle for controlling enzyme–substrate interactions	4
Virus inactivation at moderately low pH varies with virus and buffer properties	4
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Studying the impact of cell age on the yeast growth behavior of Saccharomyces pastorianus var. carlsbergensis by magnetic separation	4
Combination of MAR and intron increase transgene expression of episomal vectors in CHO cells	4
Outside Front Cover: (Biotechnology Journal 2/2021)	4
Outside Front Cover: (Biotechnology Journal 8/2022)	4
Standardized approach for accurate and reliable model development of ion‐exchange chromatography based on parameter‐by‐parameter method and consideration of extra‐column effects	4
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production	4
Regeneration of Capto Core 700 resin through high throughput and laboratory scale studies and impact on production of a SARS‐CoV‐2 vaccine candidate	4
A refined Uni‐vector prime editing system improves genome editing outcomes in mammalian cells	4
Editorial Board: Biotechnology Journal 5/2023	4
A Competition‐Based Strategy for the Isolation of an Anti‐Idiotypic Blocking Module and Fine‐Tuning for Conditional Activation of a Therapeutic Antibody	4
Editorial: CRISPR and alternative approaches	4
Dual‐layered hydrogels allow complete genome recovery with nucleic acid cytometry	4
Outside Back Cover: (Biotechnology Journal 6/2023)	4
Outside Front Cover: (Biotechnology Journal 10/2023)	4
Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis	4
Outside Front Cover: (Biotechnology Journal 3/2023)	4
Meta‐fermentation system with a mixed culture for the production of optically pure l‐lactic acid can be reconstructed using the minimum isolates with a simplified pH control strategy	4
Comparison of protein quantification methods for protein encapsulation with ZIF‐8 metal‐organic frameworks	4
Fabrication and integration of a low‐cost 3D printing‐based glucose biosensor for bioprinted liver‐on‐a‐chip	4
Human umbilical cord‐derived mesenchymal stem cells affect urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL‐6 in a serum‐free co‐culture system	4
Outside Front Cover: (Biotechnology Journal 10/2022)	4
Considerations Regarding High Oil Density Bioreactor‐Scale Fermentations of Yarrowia lipolytica Using CFD Modeling and Experimental Validation	4
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Synergisms of machine learning and constraint‐based modeling of metabolism for analysis and optimization of fermentation parameters	4
Editorial Board: Biotechnology Journal 9/2022	4
Cross‐neutralization of RBD mutant strains of SARS‐CoV‐2 by convalescent patient derived antibodies	4
Outside Back Cover: (Biotechnology Journal 11/2023)	4
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Improvement of the performance of anticancer peptides using a drug repositioning pipeline	4
Editorial Board: Biotechnology Journal 2/2021	4
Physiochemical properties of enveloped viruses and arginine dictate inactivation	4
Key criteria for engineering mycotoxin binding aptamers via computational simulations: Aflatoxin B1 as a case study	4
Engineering of TEV protease variants with redesigned substrate specificity	4
Three‐dimensional spheroid formation of adipose‐derived stem cells improves the survival of fat transplantation by enhance their therapeutic effect	4
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Lactobacillus reuteri Assists Engineered Bacteria That Target Tumors to Release PD‐L1nb to Mitigate the Adverse Effects of Breast Cancer Immunotherapy	4