Biotechnology Journal

Papers
(The TQCC of Biotechnology Journal is 5. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-11-01 to 2024-11-01.)
ArticleCitations
Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (stinging nettle) leaf extract: Antimicrobial and anticancer activity100
Biohydrogen production from microalgae—Major bottlenecks and future research perspectives88
Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment78
Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates74
Surface engineering of biomaterials in orthopedic and dental implants: Strategies to improve osteointegration, bacteriostatic and bactericidal activities73
Advances in promoter engineering: Novel applications and predefined transcriptional control64
Quality Control Testing, Characterization and Critical Quality Attributes of Adeno‐Associated Virus Vectors Used for Human Gene Therapy55
Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine49
Engineering Tobacco Mosaic Virus and Its Virus‐Like‐Particles for Synthesis of Biotemplated Nanomaterials41
Advances in Lentivirus Purification40
Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions38
Engineering the oleaginous yeast Yarrowia lipolytica for β‐farnesene overproduction37
Advancements in molecular design and bioprocessing of recombinant adeno‐associated virus gene delivery vectors using the insect‐cell baculovirus expression platform34
Detection of the SARS‐CoV‐2 D614G mutation using engineered Cas12a guide RNA31
Generation of enzymatically competent SARS‐CoV‐2 decoy receptor ACE2‐Fc in glycoengineered Nicotiana benthamiana29
Production of MSTN‐mutated cattle without exogenous gene integration using CRISPR‐Cas929
Xylose Assimilation for the Efficient Production of Biofuels and Chemicals by Engineered Saccharomyces cerevisiae28
Dissolvable Gelatin‐Based Microcarriers Generated through Droplet Microfluidics for Expansion and Culture of Mesenchymal Stromal Cells26
Influence of biosynthesized magnesium oxide nanoparticles on growth and physiological aspects of cowpea (Vigna unguiculata L.) plant, cowpea beetle, and cytotoxicity25
Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads25
Hydroxypropyl methylcellulose/graphene oxide composite as drug carrier system for 5‐fluorouracil23
Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges22
Synergisms of machine learning and constraint‐based modeling of metabolism for analysis and optimization of fermentation parameters22
Real‐time amino acid and glucose monitoring system for the automatic control of nutrient feeding in CHO cell culture using Raman spectroscopy22
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS‐1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle22
Engineering Acetobacterium woodii for the production of isopropanol and acetone from carbon dioxide and hydrogen21
Three‐dimensional‐poly(lactic acid) scaffolds coated with gelatin/magnesium‐doped nano‐hydroxyapatite for bone tissue engineering20
Identification and analysis of sugar transporters capable of co‐transporting glucose and xylose simultaneously20
Naming CHO cells for bio‐manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names19
Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles19
Lyophilization stabilizes clinical‐stage core‐crosslinked polymeric micelles to overcome cold chain supply challenges19
Dimethyloxalylglycine, a small molecule, synergistically increases the homing and angiogenic properties of human mesenchymal stromal cells when cultured as 3D spheroids19
L‐malic acid production from xylose by engineered Saccharomyces cerevisiae19
Chitosan and silver nanoparticles are attractive auxin carriers: A comparative study on the adventitious rooting of microcuttings in apple rootstocks18
Factors and methods to modulate DNA hybridization kinetics18
Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV‐1 Infection18
A deep learning approach to evaluate the feasibility of enzymatic reactions generated by retrobiosynthesis18
Optimization of muscle cell culture media using nonlinear design of experiments18
Short‐chain alcohols inactivate an immobilized industrial lipase through two different mechanisms17
Comparison of mechanistic and hybrid modeling approaches for characterization of a CHO cultivation process: Requirements, pitfalls and solution paths16
Data integrity within the biopharmaceutical sector in the era of Industry 4.016
An integrative multiomics approach to characterize anti‐adipogenic and anti‐lipogenic effects of Akkermansia muciniphila in adipocytes16
Complete or periodic continuity in continuous manufacturing platforms for production of monoclonal antibodies?15
Expanding the upper‐temperature boundary for the microalga Picochlorum sp. (BPE23) by adaptive laboratory evolution15
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae15
Simultaneous production of 2′‐fucosyllactose and difucosyllactose by engineered Escherichia coli with high secretion efficiency15
Application of microfluidic technology in antibody screening15
Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis15
Epitope screening using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX‐MS): An accelerated workflow for evaluation of lead monoclonal antibodies15
Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies15
Selective ene‐reductase immobilization to magnetic nanoparticles through a novel affinity tag15
Exosomes: Biogenesis, targeting, characterization and their potential as "Plug & Play" vaccine platforms14
Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells14
Intravenous injection of mesenchymal stem cell spheroids improves the pulmonary delivery and prolongs in vivo survival14
Insight into the bioabsorption of Fe‐based materials and their current developments in bone applications14
Simplifying plant gene silencing and genome editing logistics by a one‐Agrobacterium system for simultaneous delivery of multipartite virus vectors14
Process economics evaluation of cell‐free synthesis for the commercial manufacture of antibody drug conjugates14
Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review14
Targeting metabolic driving and minimization of by‐products synthesis for high‐yield production of D‐pantothenate in Escherichia coli14
Highly efficient expression and secretion of human lysozyme using multiple strategies in Pichia pastoris14
Microfluidic fabrication of tunable alginate‐based microfibers for the stable immobilization of enzymes14
Novel anion exchange membrane chromatography method for the separation of empty and full adeno‐associated virus14
Definitive screening accelerates Taxol biosynthetic pathway optimization and scale up in Saccharomyces cerevisiae cell factories14
Error‐free recombination in sugarcane mediated by only 30 nucleotides of homology and CRISPR/Cas9 induced DNA breaks or Cre‐recombinase13
Key advances in biocatalytic phosphorylations in the last two decades: Biocatalytic syntheses in vitro and biotransformations in vivo (in humans)13
Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance13
Biotechnological production of the European corn borer sex pheromone in the yeast Yarrowia lipolytica12
Assessing the Combinatorial Chemo‐Photothermal Therapy Mediated by Sulfobetaine Methacrylate‐Functionalized Nanoparticles in 2D and 3D In Vitro Cancer Models12
Rational engineering and applications of functional bioadhesives in biomedical engineering12
A combined pH and temperature precipitation step facilitates the purification of tobacco‐derived recombinant proteins that are sensitive to extremes of either parameter12
Improvement of acetyl‐CoA supply and glucose utilization increases l‐leucine production in Corynebacterium glutamicum12
Nitrilase immobilization and transposition from a micro‐scale batch to a continuous process increase the nicotinic acid productivity12
Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining12
Advances in production and structural derivatization of the promising molecule ursolic acid12
High cysteine concentrations in cell culture media lead to oxidative stress and reduced bioprocess performance of recombinant CHO cells12
An efficient cell‐free protein synthesis platform for producing proteins with pyrrolysine‐based noncanonical amino acids12
Donepezil hydrochloride as a novel inducer for osteogenic differentiation of mesenchymal stem cells on PLLA scaffolds in vitro12
Optimization of CRISPR‐Cas9 through promoter replacement and efficient production of L‐homoserine in Corynebacterium glutamicum11
Microaerobic growth‐decoupled production of α‐ketoglutarate and succinate from xylose in a one‐pot process using Corynebacterium glutamicum11
Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae11
Longitudinal monitoring of cell metabolism in biopharmaceutical production using label‐free fluorescence lifetime imaging microscopy11
Auxotrophs compromise cell growth and fatty acid production in Saccharomyces cerevisiae11
Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells11
Boosting production of cembratriene‐ol in Saccharomyces cerevisiae via systematic optimization11
Single‐strand annealing: Molecular mechanisms and potential applications in CRISPR‐Cas‐based precision genome editing11
CRISPR/Cas9‐mediated knockout of a prolyl‐4‐hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection11
Building a machine‐learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast11
Enzyme selection, optimization, and production toward biodegradation of post‐consumer poly(ethylene terephthalate) at scale11
Systematizing the different notions of growth‐coupled product synthesis and a single framework for computing corresponding strain designs11
Designed SARS‐CoV‐2 receptor binding domain variants form stable monomers10
Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks10
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production10
Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives10
Comprehensive characterization of dihydrofolate reductase‐mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies10
Cell‐free in vitro reduction of carboxylates to aldehydes: With crude enzyme preparations to a key pharmaceutical building block10
Accelerating HIV‐1 VLP production using stable High Five insect cell pools10
Assessing the potential of Schizochytrium sp. HX‐308 for microbial lipids production from corn stover hydrolysate10
Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells10
IPTG‐induced high protein expression for whole‐cell biosynthesis of L‐phosphinothricin10
High fidelity one‐pot DNA assembly using orthogonal serine integrases10
Carotenoid fortification of zucchini fruits using a viral RNA vector9
Methods for addressing host cell protein impurities in biopharmaceutical product development9
Key criteria for engineering mycotoxin binding aptamers via computational simulations: Aflatoxin B1 as a case study9
Biotransformation of ethylene glycol to glycolic acid by Yarrowia lipolytica: A route for poly(ethylene terephthalate) (PET) upcycling9
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli9
Near‐infrared light responsive dendrimers facilitate the extraction of bicalutamide from human plasma and urine9
Cell‐surface binding domains from Clostridium cellulovorans can be used for surface display of cellulosomal scaffoldins in Lactococcus lactis9
Chemical modification of lipases: A powerful tool for activity improvement9
An engineered microfluidic blood‐brain barrier model to evaluate the anti‐metastatic activity of β‐boswellic acid9
Integrated process development: The key to improve Fab production in E. coli9
Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM19
Engineering an efficient whole‐cell catalyst for d‐allulose production from glycerol9
Degenerative disease‐on‐a‐chip: Developing microfluidic models for rapid availability of newer therapies9
Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria9
Quercetin‐loaded platelets as a potential targeted therapy for glioblastoma multiforme cell line U373‐MG9
Comprehensive mRNA‐sequencing‐based characterization of three HEK‐293 cell lines during an rAAV production process for gene therapy applications9
GFP‐tagging of extracellular vesicles for rapid process development8
The SSV‐Seq 2.0 PCR‐Free Method Improves the Sequencing of Adeno‐Associated Viral Vector Genomes Containing GC‐Rich Regions and Homopolymers8
Supplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli8
Neuronal circuits on a chip for biological network monitoring8
Converting a mAb downstream process from batch to continuous using process modeling and process analytical technology8
Biocatalytic synthesis of 2‐Oα‐D‐glucopyranosyl‐L‐ascorbic acid using an extracellular expressed α‐glucosidase from Oryza sativa8
The role of the CRISPR‐Cas system in cancer drug development: Mechanisms of action and therapy8
Physiochemical properties of enveloped viruses and arginine dictate inactivation8
Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: Implications for baculovirus expression8
Recent advances in the microbial production of C4 alcohols by metabolically engineered microorganisms8
Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi‐step assurance of monoclonality8
A BioBricks toolbox for metabolic engineering of the tetracenomycin pathway8
Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production8
Optimization of a transient antibody expression platform towards high titer and efficiency8
Biofabrication of a three dimensional human‐based personalized neurofibroma model8
Vaccine and vaccination as a part of human life: In view of COVID‐198
Engineering a thermostable chondroitinase for production of specifically distributed low‐molecular‐weight chondroitin sulfate8
Fluorescence‐based screening for engineered aldo‐keto reductase KmAKR with improved catalytic performance and extended substrate scope8
Umbilical cord derived mesenchymal stromal cells in microcarrier based industrial scale culture sustain the immune regulatory functions8
Advances in three dimensional metal enhanced fluorescence based biosensors using metal nanomaterial and nano‐patterned surfaces8
Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation8
Rational hinge engineering of carboxylic acid reductase from Mycobacterium smegmatis enhances its catalytic efficiency in biocatalysis8
Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity8
Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality8
Genome Editing‐accelerated Re‐Domestication (GEaReD) – A new major direction in plant breeding8
Heterologous expression of cyanobacterial gas vesicle proteins in Saccharomyces cerevisiae8
Reactor control system in bacterial co‐culture based on fluorescent proteins using an Arduino‐based home‐made device8
Parallel and large‐scale antitumor investigation using stable chemical gradient and heterotypic three‐dimensional tumor coculture in a multi‐layered microfluidic device8
Rsn‐2‐mediated directed foam enrichment of β‐lactamase7
Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora<7
Enhanced production of nonanedioic acid from nonanoic acid by engineered Escherichia coli7
An integrated modular framework for modeling the effect of ammonium on the sialylation process of monoclonal antibodies produced by CHO cells7
Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line7
Lyophilization of premixed COVID‐19 diagnostic RT‐qPCR reactions enables stable long‐term storage at elevated temperature7
Reducing water uptake into BY‐2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs7
Spinal cord regeneration: A brief overview of the present scenario and a sneak peek into the future7
Advancing Raman model calibration for perfusion bioprocesses using spiked harvest libraries7
Rapid regulations of metabolic reactions in Escherichia coli via light‐responsive enzyme redistribution7
Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells7
Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters7
Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva7
CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome7
Medium optimization and subsequent fermentative regulation enabled the scaled‐up production of anti‐tuberculosis drug leads ilamycin‐E1/E27
Computational fluid dynamics simulation improves the design and characterization of a plug‐flow‐type scale‐down reactor for microbial cultivation processes7
Enhancing cereal productivity by genetic modification of root architecture7
A highly sensitive PNA‐microarray system for miRNA122 recognition7
Mechanistic discoveries and simulation‐guided assay optimization of portable hormone biosensors with cell‐free protein synthesis7
Cross‐neutralization of RBD mutant strains of SARS‐CoV‐2 by convalescent patient derived antibodies7
Novel erythropoietin‐based therapeutic candidates with extra N‐glycan sites that block hematopoiesis but preserve neuroplasticity7
Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation7
Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology6
Virus inactivation at moderately low pH varies with virus and buffer properties6
Highly efficient CRISPR‐mediated base editing for the gut Bacteroides spp. with pnCasBS‐CBE6
Immobilization of transgenic plant cells towards bioprinting for production of a recombinant biodefense agent6
Integrating spheroid‐on‐a‐chip with tubeless rocker platform: A high‐throughput biological screening platform6
Enhancing the organic solvent resistance of ω‐amine transaminase for enantioselective synthesis of (R)‐(+)‐1(1‐naphthyl)‐ethylamine6
l‐Lactate oxidase‐mediated removal of l‐lactic acid derived from fermentation medium for the production of optically pure D‐lactic acid6
Microwell‐based flow culture increases viability and restores drug response in prostate cancer spheroids6
Magnetic resonance imaging as a tool for quality control in extrusion‐based bioprinting6
CRISPR‐Cas enzymes: The toolkit revolutionizing diagnostics6
Comparison of protein quantification methods for protein encapsulation with ZIF‐8 metal‐organic frameworks6
Direct capture and selective elution of a secreted polyglutamate‐tagged nanobody using bare magnetic nanoparticles6
The prediction model of nitrogen nutrition in cotton canopy leaves based on hyperspectral visible‐near infrared band feature fusion6
Metabolic regulation and optimization of oxygen supply enhance the 2,3‐butanediol yield of the novel Klebsiella sp. isolate FSoil 0246
Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production6
Using lysozyme amyloid fibrils as a means of scavenging aggregation‐inhibiting compounds6
Designing microfluidic devices for behavioral screening of multiple zebrafish larvae6
Breakthrough in CRISPR/Cas system: Current and future directions and challenges6
Surrogate model to screen for inactivation‐based clearance of enveloped viruses during biotherapeutics process development6
Immobilized‐laccase bioreactors for wastewater treatment6
Improving Antibody Production in Stably Transfected CHO Cells by CRISPR‐Cas9‐Mediated Inactivation of Genes Identified in a Large‐Scale Screen with Chinese Hamster‐Specific siRNAs6
A tailored LNA clamping design principle: Efficient, economized, specific and ultrasensitive for the detection of point mutations6
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications6
Climate‐Simulated culturing suggests high microalgal biomass and oil productivities in most of the South American continent6
Transporter mining and metabolic engineering of Escherichia coli for high‐level D‐allulose production from D‐fructose by thermo‐swing fermentation6
A seasonal simulation approach for culture depth influence on the temperature for different characterized microalgae strains6
High production of jasmonic acid by Lasiodiplodia iranensis using solid‐state fermentation: Optimization and understanding6
A biodegradable, mechanically tunable micro‐arc oxidation AZ91D‐based composite implant with calcium phosphate/chitosan coating promotes long‐term bone tissue regeneration6
Improved extraction and purification of the hydrophobin HFBI5
Meta‐fermentation system with a mixed culture for the production of optically pure l‐lactic acid can be reconstructed using the minimum isolates with a simplified pH control strategy5
Protoplast transformation of Kluyveromyces marxianus5
State of the art of bone biomaterials and their interactions with stem cells: Current state and future directions5
Secretory production of 7‐dehydrocholesterol by engineered Saccharomyces cerevisiae5
Emerging Strategies in Anticancer Combination Therapy Employing Silica‐Based Nanosystems5
The impact of expression vector position on transgene transcription allows for rational expression vector design in a targeted integration system5
Single in‐line biomass probe detects CHO cell growth by capacitance and bacterial contamination by conductivity in bioreactor5
Compartmentalized microfluidic device for in vitro co‐culture of retinal cells5
Improving ribonucleic acid production in Saccharomyces pastorianus via in silico genome‐scale metabolic network model5
Media engineering in marine diatom Phaeodactylum tricornutum employing cost‐effective substrates for sustainable production of high‐value renewables5
Assessing the predictive capabilities of design heuristics and coarse‐grain simulation toward understanding and optimizing site‐specific covalent immobilization of β‐lactamase5
Conversion of five proluciferin esters by human cytochrome P450 enzymes5
Human umbilical cord‐derived mesenchymal stem cells affect urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL‐6 in a serum‐free co‐culture system5
Enhancement of rAAV titers via inhibition of the interferon signaling cascade in transfected HEK293 suspension cultures5
Adapting microalgae‐based strategies for sustainable green cities5
A bioengineered pseudovirus nanoparticle displaying SARS‐CoV 2 RBD fully protects mice from mortality and weight loss caused by SARS‐CoV 2 challenge5
Improved synthesis of dietary triglycerides by using lipase supported on clay carriers5
Mixing and oxygen transfer characteristics of a microplate bioreactor with surface‐attached microposts5
Nanomaterials for Biomedical Applications5
Cellulase mediated stress triggers the mutations of oleaginous yeast Trichosporon cutaneum with super‐large spindle morphology and high lipid accumulation5
Aptamer‐modified tetrahedral DNA nanostructure‐immobilized liposome for specific gene delivery and potential cancer theragnostic5
Synthetic logic circuits using RNA aptamer against T7 RNA polymerase5
Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis5
Multiomics analysis reveals the biological effects of live Roseburia intestinalis as a high‐butyrate‐producing bacterium in human intestinal epithelial cells5
BMP‐2/TGF‐β1 gene insertion into ligament‐derived stem cells sheet promotes tendon–bone healing in a mouse5
Indigenous microalgae strains characterization for a sustainable biodiesel production5
Enhanced production of difficult‐to‐express proteins through knocking down rnpA gene expression5
Production of adeno‐associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell densities5
Characterization of essential elements for improved episomal expressions in Kluyveromyces marxianus5
Bimetallic hydrogels based on chitosan and carrageenan as promising materials for biological applications5
Enhanced single‐base mutation diversity by the combination of cytidine deaminase with DNA‐repairing enzymes in yeast5
A novel therapeutic phosphate‐based glass improves full‐thickness wound healing in a rat model5
Isolation of extracellular vesicles from human plasma samples: The importance of controls5
Shell engineering in soft alginate‐based capsules for culturing liver spheroids5
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