Biotechnology Journal

Article	Citations
Green and ecofriendly biosynthesis of selenium nanoparticles using Urtica dioica (stinging nettle) leaf extract: Antimicrobial and anticancer activity	100
Biohydrogen production from microalgae—Major bottlenecks and future research perspectives	88
Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment	78
Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates	74
Surface engineering of biomaterials in orthopedic and dental implants: Strategies to improve osteointegration, bacteriostatic and bactericidal activities	73
Advances in promoter engineering: Novel applications and predefined transcriptional control	64
Quality Control Testing, Characterization and Critical Quality Attributes of Adeno‐Associated Virus Vectors Used for Human Gene Therapy	55
Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine	49
Engineering Tobacco Mosaic Virus and Its Virus‐Like‐Particles for Synthesis of Biotemplated Nanomaterials	41
Advances in Lentivirus Purification	40
Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions	38
Engineering the oleaginous yeast Yarrowia lipolytica for β‐farnesene overproduction	37
Advancements in molecular design and bioprocessing of recombinant adeno‐associated virus gene delivery vectors using the insect‐cell baculovirus expression platform	34
Detection of the SARS‐CoV‐2 D614G mutation using engineered Cas12a guide RNA	31
Production of MSTN‐mutated cattle without exogenous gene integration using CRISPR‐Cas9	29
Generation of enzymatically competent SARS‐CoV‐2 decoy receptor ACE2‐Fc in glycoengineered Nicotiana benthamiana	29
Xylose Assimilation for the Efficient Production of Biofuels and Chemicals by Engineered Saccharomyces cerevisiae	28
Dissolvable Gelatin‐Based Microcarriers Generated through Droplet Microfluidics for Expansion and Culture of Mesenchymal Stromal Cells	26
Influence of biosynthesized magnesium oxide nanoparticles on growth and physiological aspects of cowpea (Vigna unguiculata L.) plant, cowpea beetle, and cytotoxicity	25
Antimicrobial and antiviral activities with molecular docking study of chitosan/carrageenan@clove oil beads	25
Hydroxypropyl methylcellulose/graphene oxide composite as drug carrier system for 5‐fluorouracil	23
Synergisms of machine learning and constraint‐based modeling of metabolism for analysis and optimization of fermentation parameters	22
Real‐time amino acid and glucose monitoring system for the automatic control of nutrient feeding in CHO cell culture using Raman spectroscopy	22
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS‐1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle	22
Unraveling the promise and limitations of CRISPR/Cas system in natural product research: Approaches and challenges	22

Engineering Acetobacterium woodii for the production of isopropanol and acetone from carbon dioxide and hydrogen	21
Identification and analysis of sugar transporters capable of co‐transporting glucose and xylose simultaneously	20
Three‐dimensional‐poly(lactic acid) scaffolds coated with gelatin/magnesium‐doped nano‐hydroxyapatite for bone tissue engineering	20
Lyophilization stabilizes clinical‐stage core‐crosslinked polymeric micelles to overcome cold chain supply challenges	19
Dimethyloxalylglycine, a small molecule, synergistically increases the homing and angiogenic properties of human mesenchymal stromal cells when cultured as 3D spheroids	19
L‐malic acid production from xylose by engineered Saccharomyces cerevisiae	19
Naming CHO cells for bio‐manufacturing: Genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names	19
Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles	19
Chitosan and silver nanoparticles are attractive auxin carriers: A comparative study on the adventitious rooting of microcuttings in apple rootstocks	18
Factors and methods to modulate DNA hybridization kinetics	18
Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV‐1 Infection	18
A deep learning approach to evaluate the feasibility of enzymatic reactions generated by retrobiosynthesis	18
Optimization of muscle cell culture media using nonlinear design of experiments	18
Short‐chain alcohols inactivate an immobilized industrial lipase through two different mechanisms	17
Comparison of mechanistic and hybrid modeling approaches for characterization of a CHO cultivation process: Requirements, pitfalls and solution paths	16
Data integrity within the biopharmaceutical sector in the era of Industry 4.0	16
An integrative multiomics approach to characterize anti‐adipogenic and anti‐lipogenic effects of Akkermansia muciniphila in adipocytes	16
Simultaneous production of 2′‐fucosyllactose and difucosyllactose by engineered Escherichia coli with high secretion efficiency	15
Application of microfluidic technology in antibody screening	15
Detection of four foodborne pathogens based on magnetic separation multiplex PCR and capillary electrophoresis	15
Epitope screening using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX‐MS): An accelerated workflow for evaluation of lead monoclonal antibodies	15
Subcloning induces changes in the DNA‐methylation pattern of outgrowing Chinese hamster ovary cell colonies	15
Selective ene‐reductase immobilization to magnetic nanoparticles through a novel affinity tag	15
Complete or periodic continuity in continuous manufacturing platforms for production of monoclonal antibodies?	15
Expanding the upper‐temperature boundary for the microalga Picochlorum sp. (BPE23) by adaptive laboratory evolution	15
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae	15
Simplifying plant gene silencing and genome editing logistics by a one‐Agrobacterium system for simultaneous delivery of multipartite virus vectors	14
Process economics evaluation of cell‐free synthesis for the commercial manufacture of antibody drug conjugates	14
Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review	14
Targeting metabolic driving and minimization of by‐products synthesis for high‐yield production of D‐pantothenate in Escherichia coli	14
Highly efficient expression and secretion of human lysozyme using multiple strategies in Pichia pastoris	14
Microfluidic fabrication of tunable alginate‐based microfibers for the stable immobilization of enzymes	14
Novel anion exchange membrane chromatography method for the separation of empty and full adeno‐associated virus	14
Definitive screening accelerates Taxol biosynthetic pathway optimization and scale up in Saccharomyces cerevisiae cell factories	14
Exosomes: Biogenesis, targeting, characterization and their potential as "Plug & Play" vaccine platforms	14
Hydrostatic pressure promotes chondrogenic differentiation and microvesicle release from human embryonic and bone marrow stem cells	14
Intravenous injection of mesenchymal stem cell spheroids improves the pulmonary delivery and prolongs in vivo survival	14
Insight into the bioabsorption of Fe‐based materials and their current developments in bone applications	14
Key advances in biocatalytic phosphorylations in the last two decades: Biocatalytic syntheses in vitro and biotransformations in vivo (in humans)	13
Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance	13
Error‐free recombination in sugarcane mediated by only 30 nucleotides of homology and CRISPR/Cas9 induced DNA breaks or Cre‐recombinase	13
Assessing the Combinatorial Chemo‐Photothermal Therapy Mediated by Sulfobetaine Methacrylate‐Functionalized Nanoparticles in 2D and 3D In Vitro Cancer Models	12
Rational engineering and applications of functional bioadhesives in biomedical engineering	12
A combined pH and temperature precipitation step facilitates the purification of tobacco‐derived recombinant proteins that are sensitive to extremes of either parameter	12
Improvement of acetyl‐CoA supply and glucose utilization increases l‐leucine production in Corynebacterium glutamicum	12
Nitrilase immobilization and transposition from a micro‐scale batch to a continuous process increase the nicotinic acid productivity	12
Humanization of Chicken‐Derived scFv Using Yeast Surface Display and NGS Data Mining	12
Advances in production and structural derivatization of the promising molecule ursolic acid	12
High cysteine concentrations in cell culture media lead to oxidative stress and reduced bioprocess performance of recombinant CHO cells	12
An efficient cell‐free protein synthesis platform for producing proteins with pyrrolysine‐based noncanonical amino acids	12

Donepezil hydrochloride as a novel inducer for osteogenic differentiation of mesenchymal stem cells on PLLA scaffolds in vitro	12
Biotechnological production of the European corn borer sex pheromone in the yeast Yarrowia lipolytica	12
Effect of vitamins and metal ions on productivity and charge heterogeneity of IgG1 expressed in CHO cells	11
Boosting production of cembratriene‐ol in Saccharomyces cerevisiae via systematic optimization	11
Single‐strand annealing: Molecular mechanisms and potential applications in CRISPR‐Cas‐based precision genome editing	11
CRISPR/Cas9‐mediated knockout of a prolyl‐4‐hydroxylase subfamily in Nicotiana benthamiana using DsRed2 for plant selection	11
Building a machine‐learning model to predict optimal mevalonate pathway gene expression levels for efficient production of a carotenoid in yeast	11
Enzyme selection, optimization, and production toward biodegradation of post‐consumer poly(ethylene terephthalate) at scale	11
Systematizing the different notions of growth‐coupled product synthesis and a single framework for computing corresponding strain designs	11
Auxotrophs compromise cell growth and fatty acid production in Saccharomyces cerevisiae	11
Optimization of CRISPR‐Cas9 through promoter replacement and efficient production of L‐homoserine in Corynebacterium glutamicum	11
Microaerobic growth‐decoupled production of α‐ketoglutarate and succinate from xylose in a one‐pot process using Corynebacterium glutamicum	11
Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae	11
Longitudinal monitoring of cell metabolism in biopharmaceutical production using label‐free fluorescence lifetime imaging microscopy	11
Comprehensive characterization of dihydrofolate reductase‐mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies	10
Cell‐free in vitro reduction of carboxylates to aldehydes: With crude enzyme preparations to a key pharmaceutical building block	10
Accelerating HIV‐1 VLP production using stable High Five insect cell pools	10
Assessing the potential of Schizochytrium sp. HX‐308 for microbial lipids production from corn stover hydrolysate	10
Establishment of a pig CRISPR/Cas9 knockout library for functional gene screening in pig cells	10
IPTG‐induced high protein expression for whole‐cell biosynthesis of L‐phosphinothricin	10
High fidelity one‐pot DNA assembly using orthogonal serine integrases	10
Designed SARS‐CoV‐2 receptor binding domain variants form stable monomers	10
Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks	10
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production	10
Increased recombinant adeno‐associated virus production by HEK293 cells using small molecule chemical additives	10
Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1	9
Engineering an efficient whole‐cell catalyst for d‐allulose production from glycerol	9
Degenerative disease‐on‐a‐chip: Developing microfluidic models for rapid availability of newer therapies	9
Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria	9
Quercetin‐loaded platelets as a potential targeted therapy for glioblastoma multiforme cell line U373‐MG	9
Comprehensive mRNA‐sequencing‐based characterization of three HEK‐293 cell lines during an rAAV production process for gene therapy applications	9
Carotenoid fortification of zucchini fruits using a viral RNA vector	9
Methods for addressing host cell protein impurities in biopharmaceutical product development	9
Key criteria for engineering mycotoxin binding aptamers via computational simulations: Aflatoxin B1 as a case study	9
Biotransformation of ethylene glycol to glycolic acid by Yarrowia lipolytica: A route for poly(ethylene terephthalate) (PET) upcycling	9
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli	9
Near‐infrared light responsive dendrimers facilitate the extraction of bicalutamide from human plasma and urine	9
Cell‐surface binding domains from Clostridium cellulovorans can be used for surface display of cellulosomal scaffoldins in Lactococcus lactis	9
Chemical modification of lipases: A powerful tool for activity improvement	9
An engineered microfluidic blood‐brain barrier model to evaluate the anti‐metastatic activity of β‐boswellic acid	9
Integrated process development: The key to improve Fab production in E. coli	9
Biofabrication of a three dimensional human‐based personalized neurofibroma model	8
Vaccine and vaccination as a part of human life: In view of COVID‐19	8
Engineering a thermostable chondroitinase for production of specifically distributed low‐molecular‐weight chondroitin sulfate	8
Fluorescence‐based screening for engineered aldo‐keto reductase KmAKR with improved catalytic performance and extended substrate scope	8
Umbilical cord derived mesenchymal stromal cells in microcarrier based industrial scale culture sustain the immune regulatory functions	8
Advances in three dimensional metal enhanced fluorescence based biosensors using metal nanomaterial and nano‐patterned surfaces	8
Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation	8
Rational hinge engineering of carboxylic acid reductase from Mycobacterium smegmatis enhances its catalytic efficiency in biocatalysis	8
Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity	8
Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality	8
Genome Editing‐accelerated Re‐Domestication (GEaReD) – A new major direction in plant breeding	8
Heterologous expression of cyanobacterial gas vesicle proteins in Saccharomyces cerevisiae	8
Reactor control system in bacterial co‐culture based on fluorescent proteins using an Arduino‐based home‐made device	8
Parallel and large‐scale antitumor investigation using stable chemical gradient and heterotypic three‐dimensional tumor coculture in a multi‐layered microfluidic device	8
GFP‐tagging of extracellular vesicles for rapid process development	8
The SSV‐Seq 2.0 PCR‐Free Method Improves the Sequencing of Adeno‐Associated Viral Vector Genomes Containing GC‐Rich Regions and Homopolymers	8
Supplementation of critical amino acids improves glycerol and lactose uptake and enhances recombinant protein production in Escherichia coli	8
Neuronal circuits on a chip for biological network monitoring	8
Converting a mAb downstream process from batch to continuous using process modeling and process analytical technology	8
Biocatalytic synthesis of 2‐O‐α‐D‐glucopyranosyl‐L‐ascorbic acid using an extracellular expressed α‐glucosidase from Oryza sativa	8
The role of the CRISPR‐Cas system in cancer drug development: Mechanisms of action and therapy	8
Physiochemical properties of enveloped viruses and arginine dictate inactivation	8
Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: Implications for baculovirus expression	8
Recent advances in the microbial production of C4 alcohols by metabolically engineered microorganisms	8
Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi‐step assurance of monoclonality	8
A BioBricks toolbox for metabolic engineering of the tetracenomycin pathway	8
Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production	8
Optimization of a transient antibody expression platform towards high titer and efficiency	8
Rapid regulations of metabolic reactions in Escherichia coli via light‐responsive enzyme redistribution	7
Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells	7
Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters	7
Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva	7
CpPosNeg: A positive‐negative selection strategy allowing multiple cycles of marker‐free engineering of the Chlamydomonas plastome	7
Medium optimization and subsequent fermentative regulation enabled the scaled‐up production of anti‐tuberculosis drug leads ilamycin‐E1/E2	7
Computational fluid dynamics simulation improves the design and characterization of a plug‐flow‐type scale‐down reactor for microbial cultivation processes	7
Enhancing cereal productivity by genetic modification of root architecture	7
A highly sensitive PNA‐microarray system for miRNA122 recognition	7
Mechanistic discoveries and simulation‐guided assay optimization of portable hormone biosensors with cell‐free protein synthesis	7
Cross‐neutralization of RBD mutant strains of SARS‐CoV‐2 by convalescent patient derived antibodies	7

Novel erythropoietin‐based therapeutic candidates with extra N‐glycan sites that block hematopoiesis but preserve neuroplasticity	7
Capillary and microchip electrophoresis method development for amino acid monitoring during biopharmaceutical cultivation	7
Rsn‐2‐mediated directed foam enrichment of β‐lactamase	7
Prevention of nosocomial transmission and biofilm formation on novel biocompatible antimicrobial gloves impregnated with biosynthesized silver nanoparticles synthesized using Eucalyptus citriodora<	7
Enhanced production of nonanedioic acid from nonanoic acid by engineered Escherichia coli	7
An integrated modular framework for modeling the effect of ammonium on the sialylation process of monoclonal antibodies produced by CHO cells	7
Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line	7
Lyophilization of premixed COVID‐19 diagnostic RT‐qPCR reactions enables stable long‐term storage at elevated temperature	7
Reducing water uptake into BY‐2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs	7
Spinal cord regeneration: A brief overview of the present scenario and a sneak peek into the future	7
Advancing Raman model calibration for perfusion bioprocesses using spiked harvest libraries	7
Integrating spheroid‐on‐a‐chip with tubeless rocker platform: A high‐throughput biological screening platform	6
Enhancing the organic solvent resistance of ω‐amine transaminase for enantioselective synthesis of (R)‐(+)‐1(1‐naphthyl)‐ethylamine	6
l‐Lactate oxidase‐mediated removal of l‐lactic acid derived from fermentation medium for the production of optically pure D‐lactic acid	6
Microwell‐based flow culture increases viability and restores drug response in prostate cancer spheroids	6
Magnetic resonance imaging as a tool for quality control in extrusion‐based bioprinting	6
CRISPR‐Cas enzymes: The toolkit revolutionizing diagnostics	6
Improving Antibody Production in Stably Transfected CHO Cells by CRISPR‐Cas9‐Mediated Inactivation of Genes Identified in a Large‐Scale Screen with Chinese Hamster‐Specific siRNAs	6
A tailored LNA clamping design principle: Efficient, economized, specific and ultrasensitive for the detection of point mutations	6
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications	6
Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production	6
Using lysozyme amyloid fibrils as a means of scavenging aggregation‐inhibiting compounds	6
Designing microfluidic devices for behavioral screening of multiple zebrafish larvae	6
Breakthrough in CRISPR/Cas system: Current and future directions and challenges	6
Surrogate model to screen for inactivation‐based clearance of enveloped viruses during biotherapeutics process development	6
Immobilized‐laccase bioreactors for wastewater treatment	6
Virus inactivation at moderately low pH varies with virus and buffer properties	6
Highly efficient CRISPR‐mediated base editing for the gut Bacteroides spp. with pnCasBS‐CBE	6
Immobilization of transgenic plant cells towards bioprinting for production of a recombinant biodefense agent	6
Climate‐Simulated culturing suggests high microalgal biomass and oil productivities in most of the South American continent	6
Transporter mining and metabolic engineering of Escherichia coli for high‐level D‐allulose production from D‐fructose by thermo‐swing fermentation	6
A seasonal simulation approach for culture depth influence on the temperature for different characterized microalgae strains	6
High production of jasmonic acid by Lasiodiplodia iranensis using solid‐state fermentation: Optimization and understanding	6
A biodegradable, mechanically tunable micro‐arc oxidation AZ91D‐based composite implant with calcium phosphate/chitosan coating promotes long‐term bone tissue regeneration	6
Comparison of protein quantification methods for protein encapsulation with ZIF‐8 metal‐organic frameworks	6
Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology	6
Direct capture and selective elution of a secreted polyglutamate‐tagged nanobody using bare magnetic nanoparticles	6
The prediction model of nitrogen nutrition in cotton canopy leaves based on hyperspectral visible‐near infrared band feature fusion	6
Metabolic regulation and optimization of oxygen supply enhance the 2,3‐butanediol yield of the novel Klebsiella sp. isolate FSoil 024	6
Secretory production of 7‐dehydrocholesterol by engineered Saccharomyces cerevisiae	5
Emerging Strategies in Anticancer Combination Therapy Employing Silica‐Based Nanosystems	5
The impact of expression vector position on transgene transcription allows for rational expression vector design in a targeted integration system	5
Single in‐line biomass probe detects CHO cell growth by capacitance and bacterial contamination by conductivity in bioreactor	5
Compartmentalized microfluidic device for in vitro co‐culture of retinal cells	5
Improving ribonucleic acid production in Saccharomyces pastorianus via in silico genome‐scale metabolic network model	5
Media engineering in marine diatom Phaeodactylum tricornutum employing cost‐effective substrates for sustainable production of high‐value renewables	5
Assessing the predictive capabilities of design heuristics and coarse‐grain simulation toward understanding and optimizing site‐specific covalent immobilization of β‐lactamase	5
Conversion of five proluciferin esters by human cytochrome P450 enzymes	5
Human umbilical cord‐derived mesenchymal stem cells affect urea synthesis and the cell apoptosis of human induced hepatocytes by secreting IL‐6 in a serum‐free co‐culture system	5
Enhancement of rAAV titers via inhibition of the interferon signaling cascade in transfected HEK293 suspension cultures	5
Adapting microalgae‐based strategies for sustainable green cities	5
A bioengineered pseudovirus nanoparticle displaying SARS‐CoV 2 RBD fully protects mice from mortality and weight loss caused by SARS‐CoV 2 challenge	5
Improved synthesis of dietary triglycerides by using lipase supported on clay carriers	5
Mixing and oxygen transfer characteristics of a microplate bioreactor with surface‐attached microposts	5
Nanomaterials for Biomedical Applications	5
Cellulase mediated stress triggers the mutations of oleaginous yeast Trichosporon cutaneum with super‐large spindle morphology and high lipid accumulation	5
Aptamer‐modified tetrahedral DNA nanostructure‐immobilized liposome for specific gene delivery and potential cancer theragnostic	5
Synthetic logic circuits using RNA aptamer against T7 RNA polymerase	5
Application of adaptive laboratory evolution to improve the tolerance of Rhodotorula strain to methanol in crude glycerol and development of an effective method for cell lysis	5
Multiomics analysis reveals the biological effects of live Roseburia intestinalis as a high‐butyrate‐producing bacterium in human intestinal epithelial cells	5
BMP‐2/TGF‐β1 gene insertion into ligament‐derived stem cells sheet promotes tendon–bone healing in a mouse	5
Indigenous microalgae strains characterization for a sustainable biodiesel production	5
Enhanced production of difficult‐to‐express proteins through knocking down rnpA gene expression	5
Production of adeno‐associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell densities	5
Characterization of essential elements for improved episomal expressions in Kluyveromyces marxianus	5
Bimetallic hydrogels based on chitosan and carrageenan as promising materials for biological applications	5
Enhanced single‐base mutation diversity by the combination of cytidine deaminase with DNA‐repairing enzymes in yeast	5
A novel therapeutic phosphate‐based glass improves full‐thickness wound healing in a rat model	5
Isolation of extracellular vesicles from human plasma samples: The importance of controls	5
Shell engineering in soft alginate‐based capsules for culturing liver spheroids	5
Improved extraction and purification of the hydrophobin HFBI	5
Meta‐fermentation system with a mixed culture for the production of optically pure l‐lactic acid can be reconstructed using the minimum isolates with a simplified pH control strategy	5
Protoplast transformation of Kluyveromyces marxianus	5
State of the art of bone biomaterials and their interactions with stem cells: Current state and future directions	5
Transforming native exosomes to engineered drug vehicles: A smart solution to modern cancer theranostics	4
Improving bioluminescence of a minimal luciferase by adding a charged oligopeptide: picALuc2.0	4
Light‐induced fermenter production of derivatives of the sweet protein monellin is maximized in prestationary Saccharomyces cerevisiae cultures	4
Optimizing VLP production in gene therapy: Opportunities and challenges for in silico modeling	4
Sequence mining yields 18 phloretin C‐glycosyltransferases from plants for the efficient biocatalytic synthesis of nothofagin and phloretin‐di‐C‐glycoside	4
Regeneration of Capto Core 700 resin through high throughput and laboratory scale studies and impact on production of a SARS‐CoV‐2 vaccine candidate	4
1H NMR‐based process understanding and biochemical marker identification methodology for monitoring CHO cell culture process during commercial‐scale manufacturing	4
Accelerating wound healing: Unveiling synergistic effects of P25/SWCNT/Ag and P25/rGO/Ag nanocomposites within PRP‐gelatin scaffold, highlighting the synergistic antimicrobial activity	4
Recombinant protein purification and immobilization strategies based on peptides with dual affinity to iron oxide and silica	4
Error propagation in constraint‐based modeling of Chinese hamster ovary cells	4
Characterization and discrimination of spike protein in SARS‐CoV‐2 virus‐like particles via surface‐enhanced Raman spectroscopy	4
Enhancing the accumulation of lipid and docosahexaenoic acid in Schizochytrium sp. by co‐overexpression of phosphopantetheinyl transferase and ω‐3 fatty acid desaturase	4
Standardized approach for accurate and reliable model development of ion‐exchange chromatography based on parameter‐by‐parameter method and consideration of extra‐column effects	4
Rational design and experimental evaluation of peptide ligands for the purification of adeno‐associated viruses via affinity chromatography	4
Yeast‐derived vacuoles as a novel carrier with enhanced hCMEC/D3 cell monolayer penetration	4
Ultrasound assisted in situ separation of sophorolipids in multi‐phase fermentation system to achieve efficient production by Candida bombicola	4
Advances in biosynthesis of peptide drugs: Technology and industrialization	4
Rapid construction of Escherichia coli chassis with genome multi‐position integration of isopentenol utilization pathway for efficient and stable terpenoid accumulation	4
In‐silico selection employing rigid docking and molecular dynamic simulation in selecting DNA aptamers against androgen receptor	4
Microbioreactors for nutrient‐controlled microbial cultures: Bridging the gap between bioprocess development and industrial use	4
Customizing amino acid metabolism of Pichia pastoris for recombinant protein production	4