Biotechnology Journal

Papers
(The median citation count of Biotechnology Journal is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)
ArticleCitations
Advancing a rapid, high throughput screening platform for optimization of lentivirus production119
Potential of butanol production from Thailand marine macroalgae using Clostridium beijerinckii ATCC 10132‐based ABE fermentation91
Toehold switch plus signal amplification enables rapid detection79
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Cell fate‐inducing CARs orthogonally control multiple signaling pathways38
Site specific insertion of a transgene into the murine α‐casein (CSN1S1) gene results in the predictable expression of a recombinant protein in milk37
A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption30
Live‐cell imaging of genomic loci with Cas9 variants29
Osteochondral Tissue Engineering: Scaffold Materials, Fabrication Techniques and Applications29
Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d‐amino acid oxidase29
Engineering a 3‐dimensional tissue construct with adipose‐derived stem cells for healing bone defect: An ex vivo study with femur head29
Dialysis rolled scaffold bioreactor allows extended production of monoclonal antibody with reduced media use28
Biomedical application of microalgal‐biomaterials hybrid system28
Expression and application of aflatoxin degrading enzyme gene in Pichia pastoris27
Multifunctional PAMAM Dendrimers Carrying SAHA, 5‐FU, and a Therapeutic Gene for Targeted Co‐Delivery Toward Colorectal Cancer Cells27
Feeding strategies for Sporosarcina pasteurii cultivation unlock more efficient production of ureolytic biomass for MICP27
CRISPR/Cas9 mediated targeted mutagenesis of LIGULELESS‐1 in sorghum provides a rapidly scorable phenotype by altering leaf inclination angle26
Engineering of the start condensation domain with improved N‐decanoyl catalytic activity for daptomycin biosynthesis25
Overexpression of Geranylgeranyl Diphosphate Synthase and Cyclase Enhances Pleuromutilin Production in Clitopilus Passeckerianus T625
Intermediate product control in cascade reaction for one‐pot production of ε‐caprolactone by Escherichia coli24
Redesigning glycolaldehyde synthase for the synthesis of biorefinery feedstock D‐xylulose from C1 compounds24
Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae23
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications23
Scaling strategy for cell and gene therapy bioreactors based on turbulent parameters22
Improved synthesis of dietary triglycerides by using lipase supported on clay carriers22
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Bio‐based polymers containing traditional medicinal fillers for wound healing applications – An evaluation of neoteric development and future perspectives21
Outside Front Cover: (Biotechnology Journal 10/2022)21
Editorial Board: Biotechnology Journal 12/202121
Massively parallel DNA target capture using long adapter single stranded oligonucleotide (LASSO) probes assembled through a novel DNA recombinase mediated methodology20
Direct capture and selective elution of a secreted polyglutamate‐tagged nanobody using bare magnetic nanoparticles20
Conversion of five proluciferin esters by human cytochrome P450 enzymes20
In vitro acne disease model from inertial focusing effect for studying the interactions between sebocyte glands and macrophages20
A Competition‐Based Strategy for the Isolation of an Anti‐Idiotypic Blocking Module and Fine‐Tuning for Conditional Activation of a Therapeutic Antibody20
Enhancing production and purity of 9‐OH‐AD from phytosterols by balancing metabolic flux of the side‐chain degradation and 9‐position hydroxylation in Mycobacterium neoaurum20
Systematizing the different notions of growth‐coupled product synthesis and a single framework for computing corresponding strain designs19
Comparison of protein quantification methods for protein encapsulation with ZIF‐8 metal‐organic frameworks19
The evaluation of biotechnological potential of Gp144, the key molecule of natural predator bacteriophage K in Staphylococcus aureus hunting mechanism19
Combination of MAR and intron increase transgene expression of episomal vectors in CHO cells18
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Fabrication of a decellularized liver matrix–based hepatic patch for the repair of CCl4‐induced liver injury17
Microalgae‐made human vaccines and therapeutics: A decade of advances17
Metabolic engineering of Aspergillus niger for accelerated malic acid biosynthesis by improving NADPH availability16
Effect of High‐Speed Shaking on Oxygen Transfer in Shake Flasks16
A high‐fidelity, dual site‐specific integration system in CHO cells by a Bxb1 recombinase16
A new look at the fluorescent protein‐based approach for identifying optimal coding sequence for recombinant protein expression in E. coli16
Characterization and discrimination of spike protein in SARS‐CoV‐2 virus‐like particles via surface‐enhanced Raman spectroscopy16
Optimization of Isoamylase Production in Escherichia coli Under Different Nutrient Limitation Conditions16
Rapid At‐Line AAVX Affinity HPLC: Enabling Process Analytical Technology for Bioprocess Development of Adeno‐Associated Virus Vectors16
Synthetic Osmolytes for Enhanced RNA Thermotolerance and Extended Plasmid Storage at Room Temperature16
Synergisms of machine learning and constraint‐based modeling of metabolism for analysis and optimization of fermentation parameters16
Enhanced synthesis of S‐adenosyl‐L‐methionine through combinatorial metabolic engineering and Bayesian optimization in Saccharomyces cerevisiae15
Donepezil hydrochloride as a novel inducer for osteogenic differentiation of mesenchymal stem cells on PLLA scaffolds in vitro15
Dopaminergic signaling regulates zebrafish larvae's response to electricity15
Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks15
Highly efficient identification of nucleocytoplasmic O‐glycosylation by the TurboID‐based proximity labeling method in living cells15
Multiomics analysis reveals the biological effects of live Roseburia intestinalis as a high‐butyrate‐producing bacterium in human intestinal epithelial cells15
A single‐cell genomic strategy for alternative transcript start sites identification15
Yield improvement of enediyne yangpumicins in Micromonospora yangpuensis through ribosome engineering and fermentation optimization15
Single‐particle fluorescence tracking combined with TrackMate assay reveals highly heterogeneous and discontinuous lysosomal transport in freely orientated axons14
Editorial Board: Biotechnology Journal 3/202214
Improving bioluminescence of a minimal luciferase by adding a charged oligopeptide: picALuc2.014
Cas9‐orthologue‐mediated cytosine and adenine base editors recognizing NNAAAA PAM sequences14
Surrogate model to screen for inactivation‐based clearance of enveloped viruses during biotherapeutics process development14
Mimicking continuous capture chromatography for virus clearance using a single chromatography column model14
Promoter screening and identification for metabolic regulation in Acremonium chrysogenum14
Oriented in situ immobilization of a functional tyrosinase on microcrystalline cellulose effectively incorporates DOPA residues in bioengineered mussel adhesive protein14
Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line14
Single‐cell genotyping of phytoplankton from ocean water by gel‐based cell manipulation14
Outside Front Cover: (Biotechnology Journal 6/2021)13
DNA origami scaffold promoting nerve guidance and regeneration13
Investigation of the Effects of Applying Bio‐Magnesium Oxide Nanoparticle Fertilizer to Moringa Oleifera Plants on the Chemical and Vegetative Properties of the Plants’ leaves13
Expanding the targeting scope of FokI‐dCas nuclease systems with SpRY and Mb2Cas12a13
Biomass specific perfusion rate as a control lever for the continuous manufacturing of biosimilar monoclonal antibodies from CHO cell cultures13
Lipofection with Lipofectamine™ 2000 in a heparin‐free growth medium results in high transfection efficiency in chicken primordial germ cells13
Magnetic resonance imaging as a tool for quality control in extrusion‐based bioprinting13
Dinucleotide tag‐based parallel reporter gene assay method enables efficient identification of regulatory mutations12
Development of a biotechnology process for the production of a novel hyperglycosylated long‐acting recombinant bovine follicle‐stimulating hormone12
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Outside Front Cover: (Biotechnology Journal 6/2023)12
Outside Front Cover: (Biotechnology Journal 5/2023)12
Editorial Board: Biotechnology Journal 1/202312
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Dissecting cell death pathways in fed‐batch bioreactors11
Enhanced targeting: Production of filamentous bacteriophage monoclonal antibodies using the major coat protein pVIII as anchor11
A machine learning‐based approach for improving plasmid DNA production in Escherichia coli fed‐batch fermentations11
Preparation of Thermosensitive Lignocellulose Hollow Fiber Membrane Grafted With PNIPAAm and Its Application as a Cell Culture Carrier in a RSOC Dynamic Culture11
Improved adeno‐associated virus empty and full capsid separation using weak partitioning multi‐column AEX chromatography11
Lipidomic and transcriptomic analysis of the increase in eicosapentaenoic acid under cobalamin deficiency of Schizochytrium sp11
Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions11
Issue Information: Biotechnology Journal 12/202411
Exosomal lncRNA USP30‐AS1 activates the Wnt/β‐catenin signaling pathway to promote cervical cancer progression via stabilization of β‐catenin by USP3011
Continuous phase separation of stable emulsions from biphasic whole‐cell biocatalysis by catastrophic phase inversion11
Nitrilase immobilization and transposition from a micro‐scale batch to a continuous process increase the nicotinic acid productivity10
Enhancing the expression of terminal deoxynucleotidyl transferases by N‐terminal truncation10
Development and optimization of Lysis gene E as a counter‐selection marker with high stringency10
Autofluorescence prediction model for fluorescence unmixing and age determination10
Successful reversal of transgene silencing in Chlamydomonas reinhardtii10
Benchmarking and optimization of a high‐throughput sequencing based method for transgene sequence variant analysis in biotherapeutic cell line development10
The economic value of genetically engineered mosquitoes as a malaria control strategy depends on local transmission rates10
Proteomic workflows for deep phenotypic profiling of 3D organotypic liver models10
Editorial Board: Biotechnology Journal 1/202410
YBac: A novel and simple baculovirus system for heterologous protein production in Spodoptera frugiperda 9 (Sf9) cells10
Chromatin Accessibility Plays an Important Epigenetic Role on Antibody Expression From CMV Promoter and DNA Elements Flanking the CHO TI Host Landing‐Pad10
Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production9
Bioinspired 3D microprinted cell scaffolds: Integration of graph theory to recapitulate complex network wiring in lymph nodes9
Development, optimization, and scale‐up of suspension Vero cell culture process for high titer production of oncolytic herpes simplex virus‐19
mRNA Vaccines: Design Principles, Mechanisms, and Manufacturing—Insights From COVID‐19 as a Model for Combating Infectious Diseases9
Recombinant human fibroblast growth factor 7 obtained from stable Chinese hamster ovary cells enhances wound healing9
Semi‐rational engineering of D‐allulose 3‐epimerase for simultaneously improving the catalytic activity and thermostability based on D‐allulose biosensor9
Design of a microfluidic mixer channel: First steps into creating a fluorescent dye‐based biosensor for mAb aggregate detection9
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3D‐QSAR pharmacophore modeling, virtual screening, molecular docking, MD simulations, in vitro and in vivo studies to identify potential anti‐hyperplasia drugs9
Toward standardized solid medium cultivations: Online microbial monitoring based on respiration activity9
Hierarchical time‐series analysis of dynamic bioprocess systems9
Simultaneous production of 2′‐fucosyllactose and difucosyllactose by engineered Escherichia coli with high secretion efficiency9
Editorial Board: Biotechnology Journal 6/20229
Clioquinol rescues yeast cells from Aβ42 toxicity via the inhibition of oxidative damage9
Editorial Board: Biotechnology Journal 10/20219
Development of an efficient liposomal DOX delivery formulation for HCC therapy by targeting CK2α9
A 3D paper microfluidic device for enzyme‐linked assays: Application to DNA analysis9
Exploring nutrient supplementation and bioprocess optimization to improve the production of lentiviral vectors in serum‐free medium suspension cultures9
Efficient production of 22(R)‐hydroxycholesterol via combination optimization of Saccharomyces cerevisiae9
Liamocins overproduction via the two‐pH stage fermentation and anti‐Aspergillus flavus activity of Massoia lactone9
Gulo gene locus, a new gene editing locus for mammalian cells9
Generation of reproductive transgenic pigs of a CRISPR‐Cas9‐based oncogene‐inducible system by somatic cell nuclear transfer9
Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva8
Liamocin biosynthesis is induced by an autogenous host acid activation in Aureobasidium melanogenum8
Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells8
Promoting cell growth and characterizing partial symbiotic relationships in the co‐cultivation of green alga Chlamydomonas reinhardtii and Escherichia coli8
A single‐cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing8
Transcriptional Downregulation of Methanol Metabolism Key Genes During Yeast Death in Engineered Pichia pastoris8
In vitro evolution of diagnostic antibodies targeting native antigens in plasma by sandwich flow cytometry8
Initial cyclic‐di‐GMP upregulation triggers sporadic cellular expansion leading to improved cellular survival8
Integrating Biofilm‐Based Fermentation With Continuous Processes for Improved L‐Valine Production8
Recent advances and fundamentals of microalgae cultivation technology8
Comprehensive mRNA‐sequencing‐based characterization of three HEK‐293 cell lines during an rAAV production process for gene therapy applications8
Characterization of the Ubiquitin‐Modified Proteome of Recombinant Chinese Hamster Ovary Cells in Response to Endoplasmic Reticulum Stress8
Impact of Eco‐Friendly Surfactant Structure and Class on Enveloped Virus Inactivation8
A 3D bioprinted antibacterial hydrogel dressing of gelatin/sodium alginate loaded with ciprofloxacin hydrochloride8
Model‐driven design of synthetic N‐terminal coding sequences for regulating gene expression in yeast and bacteria8
Metabolic regulation and optimization of oxygen supply enhance the 2,3‐butanediol yield of the novel Klebsiella sp. isolate FSoil 0248
Editorial Board: Biotechnology Journal 4/20228
In vitro and in silico analyses reveal the interaction between LysM receptor‐like kinase3 of Solanum tuberosum and the carbohydrate elicitor Riclin octaose8
Identification of critical process parameters for expansion of clinical grade human Wharton's jelly‐derived mesenchymal stromal cells in stirred‐tank bioreactors8
Secretory production of 7‐dehydrocholesterol by engineered Saccharomyces cerevisiae8
Electrochemical Glucose Sensors: Classification, Catalyst Innovation, and Sampling Mode Evolution8
Octadecyl and sulfonyl modification of diatomite synergistically improved the immobilization efficiency of lipase and its application in the synthesis of pine sterol esters8
Editorial Board: Biotechnology Journal 8/20218
Efficient production of glutathione in Saccharomyces cerevisiae via a synthetic isozyme system8
Molecular design of controllable recombinant adeno‐associated virus (AAV) expression systems for enhanced vector production8
1H NMR‐based process understanding and biochemical marker identification methodology for monitoring CHO cell culture process during commercial‐scale manufacturing8
Evaluation of expression systems for recombinant protein production in chicken egg bioreactors8
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The Mechanism Underlying Enhanced Coal‐to‐Methane Conversion in Anaerobic Digestion With Betaine Supplementation8
Proteomic analysis reveals that the co‐ordination of cytosolic and mitochondrial pathways is beneficial for sabinene biosynthesis in engineered Saccharomyces cerevisiae8
Construction of a Cell Factory for the Targeted and Efficient Production of Phytosterol to Boldenone in Mycobacterium neoaurum8
Chicken‐Derived Single‐Chain Variable Fragments Targeting Monkeypox Virus A29L Protein8
Antibacterial functionalization of calfskin bioremediation film8
Single‐strand annealing: Molecular mechanisms and potential applications in CRISPR‐Cas‐based precision genome editing7
Phage display selection of a Pb(II) specific peptide and its application as a biorecognition unit for colorimetric detection of Pb(II) ions7
Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology7
Dual‐layered hydrogels allow complete genome recovery with nucleic acid cytometry7
Outside Front Cover: (Biotechnology Journal 12/2022)7
Outside Front Cover: (Biotechnology Journal 10/2023)7
Towards a Platform Chromatography Purification Process for Adeno‐Associated Virus (AAV)7
Biosynthesis of the antioxidant γ‐glutamyl‐cysteine with engineered Yarrowia lipolytica7
The Experimental Study of Double‐Layer Heterogeneous CA Scaffold in Promoting the Surface Shape Recovery and Internal Osteogenesis of Alveolar Bone7
Advances in promoter engineering: Novel applications and predefined transcriptional control7
Tuning mobile phase properties to improve empty and full particle separation in adeno‐associated virus productions by anion exchange chromatography7
Resolving the Trade‐Off Between Toxicity and Efficiency of CRISPR‐Cas9 System for Genome Editing Within Escherichia coli7
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Vaccine and vaccination as a part of human life: In view of COVID‐197
Concentration‐Dependent Effects of MXene Nanocomposite‐Loaded Carboxymethyl Cellulose on Wound Healing7
Development of a novel microplate for high‐throughput screening and optimization of DHA producing strains based on CFD technology7
Microfabricated dynamic brain organoid cocultures to assess the effects of surface geometry on assembloid formation7
Aptabinding of tumor necrosis factor‐α (TNFα) inhibits its proinflammatory effects and alleviates islet inflammation7
CRISPRa/i with Adaptive Single Guide Assisted Regulation DNA (ASGARD) mediated control of Chlorella sorokiniana to enhance lipid and protein production7
Rational Design of a Novel DNA Polymerase From Clostridium thermocellum to Improve LAMP Detection Performance7
Editorial Board: Biotechnology Journal 9/20217
Deletion analysis of Pichia pastoris alcohol dehydrogenase 2 (ADH2) promoter and development of synthetic promoters7
Use of tRNA gene barriers improves stability of transgene expression in CHO cells7
Cross‐neutralization of RBD mutant strains of SARS‐CoV‐2 by convalescent patient derived antibodies7
L‐malic acid production from xylose by engineered Saccharomyces cerevisiae7
The effects of length and sequence of gRNA on Cas13b and Cas13d activity in vitro and in vivo6
Ultrasound assisted in situ separation of sophorolipids in multi‐phase fermentation system to achieve efficient production by Candida bombicola6
Computational evaluation of light propagation in cylindrical bioreactors for optogenetic mammalian cell cultures6
CRISPR‐Cas enzymes: The toolkit revolutionizing diagnostics6
Implementation of RAGATH RNA‐associated DNA Endonucleases as Genome Editing Tool in Escherichia coli6
Drug‐loaded lipid nanoparticles improve the removal rates of the Staphylococcus aureus biofilm6
Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency6
Characterisation of the E. coli HMS174 and BLR host cell proteome to guide purification process development6
Lung‐specific exosomes for doxorubicin delivery in lung adenocarcinoma therapy6
Suspension culture promoted the expansion of NK‐92 cells ex vivo by enhancing the expression of IL‐2 receptor6
Rapid construction of Escherichia coli chassis with genome multi‐position integration of isopentenol utilization pathway for efficient and stable terpenoid accumulation6
In situ formation of alginic acid‐gold nanohybrids for application in cancer photothermal therapy6
A biodegradable, mechanically tunable micro‐arc oxidation AZ91D‐based composite implant with calcium phosphate/chitosan coating promotes long‐term bone tissue regeneration6
Uncertainty quantification for gene delivery methods: A roadmap for pDNA manufacturing from phase I clinical trials to commercialization6
Expanding the upper‐temperature boundary for the microalga Picochlorum sp. (BPE23) by adaptive laboratory evolution6
Study and Application of Microbial Community in Biodegradation of Kitchen Waste Using High‐Throughput Sequencing and Strain Isolation Techniques6
The immune system of prokaryotes: potential applications and implications for gene editing6
Microwell‐based flow culture increases viability and restores drug response in prostate cancer spheroids6
Recent advances in the synthesis of single‐stranded DNA in vitro6
Factors and methods to modulate DNA hybridization kinetics6
Medical Ultrasound Application Beyond Diagnosis: Insights From Ultrasound Sensing and Biological Response6
Continuous optical in‐line glucose monitoring and control in CHO cultures contributes to enhanced metabolic efficiency while maintaining darbepoetin alfa product quality6
Advances in biosynthesis of peptide drugs: Technology and industrialization6
An improved lentiviral system for efficient expression and purification of β‐defensins in mammalian cells6
Knowledge extraction from literature and enzyme sequences complements FBA analysis in metabolic engineering6
Issue Information: Biotechnology Journal 7/20246
Light‐induced fermenter production of derivatives of the sweet protein monellin is maximized in prestationary Saccharomyces cerevisiae cultures6
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Quantitative human physiology: An introduction guide for advanced tissue engineering6
Engineering phenylalanine ammonia lyase to limit feedback inhibition by cinnamate and enhance biotransformation6
Recombinant production of a highly efficient photolyase from Thermus thermophilus6
High cysteine concentrations in cell culture media lead to oxidative stress and reduced bioprocess performance of recombinant CHO cells5
Issue Information: Biotechnology Journal 4/20255
Mitochondrial Oxidative Stress Related Diagnostic Model Accurately Assesses Rheumatoid Arthritis Risk Stratification and Immune Infiltration Characterization5
Designed SARS‐CoV‐2 receptor binding domain variants form stable monomers5
A genome‐edited N. benthamiana line for industrial‐scale production of recombinant glycoproteins with targeted N‐glycosylation5
An image‐based Abplex method for high‐throughput GPCRs antibody discovery5
Establishment and application of quantitative detection of Bacillus velezensis HMB26553, a biocontrol agent against cotton damping‐off caused by Rhizoctonia5
Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family5
Development of disposable electrode for the detection of mosquito‐borne viruses5
Engineering a versatile yeast platform for sesquiterpene production from glucose or methanol5
Combination of direct boiling and glass beads increases the purity and accuracy of bacterial DNA extraction5
Editorial Board: Biotechnology Journal 2/20235
Enhancing the organic solvent resistance of ω‐amine transaminase for enantioselective synthesis of (R)‐(+)‐1(1‐naphthyl)‐ethylamine5
Tuning CpG motif position in nanostructured DNA for efficient immune stimulation5
Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi‐step assurance of monoclonality5
Tracing endogenous proteins in living cells through electrotransfer of mRNA encoding chromobodies5
Overexpression of SLC2A1, ALDOC, and PFKFB4 in the glycolysis pathway drives strong drug resistance in 3D HeLa tumor cell spheroids5
Climate‐Simulated culturing suggests high microalgal biomass and oil productivities in most of the South American continent5
A systematic evaluation of quenching and extraction procedures for quantitative metabolome profiling of HeLa carcinoma cell under 2D and 3D cell culture conditions5
Engineering an efficient whole‐cell catalyst for d‐allulose production from glycerol5
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Unlocking the formate utilization of wild‐type Yarrowia lipolytica through adaptive laboratory evolution5
A sustainable strategy for biosynthesis of Rebaudioside D using a novel glycosyltransferase of Solanum tuberosum5
Immobilization of Arg‐Gly‐Asp peptides on silk fibroin via Gly‐Ala‐Gly‐Ala‐Gly‐Ser sequences5
Exploring metabolic responses and pathway changes in CHO‐K1 cells under varied aeration conditions and copper supplementations using 1H NMR‐based metabolomics5
Designing gene manipulation schedules for high throughput parallel construction of objective strains5
Near‐infrared light responsive dendrimers facilitate the extraction of bicalutamide from human plasma and urine5
φC31 ‐Mediated cassette exchange in Sf9 insect cells for stable expression5
Outside Front Cover: (Biotechnology Journal 11/2021)5
Adaptable Manufacturing and Biofabrication of Milliscale Organ Chips With Perfusable Vascular Beds5
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