OOIR: Observatory of International Research

Papers

(The TQCC of Journal of Biological Engineering is 10. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)

Article	Citations
Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity	124
Gene syntaxes modulate gene expression and circuit behavior on plasmids	108
Self-healing PVA/PVP micelle–hydrogel composite system for potential phenytoin repositioning in wound healing	98
Systems-level analysis identifies IRF6 as an inhibitor of epithelial-mesenchymal transition	85
Nano-size tantalum particles suppress the osteogenic activity of osteoblasts and stimulate osteoclasts	79
A detoxified exosome–ECM hydrogel depot for localized and sustained chemotherapy in prostate cancer	69
Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast	64
Cerium-doped calcium carbonate microparticles combined with low-intensity ultrasound for efficient sonodynamic therapy in body sculpting	64
New approach methodologies for in vitro toxicity screening of nanomaterial using a pulmonary three-dimensional floating extracellular matrix model	56
Promoting osteogenesis and bone regeneration employing icariin-loaded nanoplatforms	56
Combinatorial engineering pinpoints shikimate pathway bottlenecks in para-aminobenzoic acid production in Pseudomonas putida	53
Integrated genomic and transcriptomic Insights into methanol tolerance mechanisms in Methylobacterium extorquens AM1, identifying key targets for strain engineering	50
Waste-to-sensor upcycling of polyethylene terephthalate over Ag/Zr-MOF photocatalyst for microplastic degradation and AI-assisted heavy metal detection	49
Cell-cultivated aquatic food products: emerging production systems for seafood	48
3D-printed nanohydroxyapatite/methylacrylylated silk fibroin scaffold for repairing rat skull defects	43
Mechanical isolation of stromal vascular fraction using a spiral-based device: cellular outcomes and point-of-care workflow considerations	43
Comparison of different decellularization protocols for porcine centrum tendineum diaphragmatis and diaphragmatic muscle – a base for effective recellularization	42
Research progress of biomimetic materials in oral medicine	41
Verification and application of an automated real-time antimicrobial susceptibility testing system: for accelerated antibiotic susceptibility testing and high-throughput screening of antibiotic synerg	41
A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy	36
Core-shell nanofiber dressings with zinc oxide nanoparticles and cell-free fat extract: boosting fibroblast activity and antibacterial efficacy	34
A stealth-body Fc engineering platform enables complete effector silencing in antibody and ADC therapeutics	33
Role of mesenchymal stem cell-derived exosomes in the regeneration of different tissues	32
Integration of TLR7/8 agonists into lipid nanoparticles enhances antigen-specific immune responses to N1-methyl-Ψ-modified mRNA-LNP vaccines	29
3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects	29

RN-Autoencoder: Reduced Noise Autoencoder for classifying imbalanced cancer genomic data	28
Quantitative imaging of magnetic nanoparticles in an unshielded environment using a large AC susceptibility array	28
Codeine 3-O-demethylase catalyzed biotransformation of morphinan alkaloids in Escherichia coli: site directed mutagenesis of terminal residues improves enzyme expression, stability and biotransformati	27
Bio-engineered thermo-sensitive alginate/PNIA-g-CS co-polymeric injectable hydrogel laden with GDF-5 to stimulate nucleus pulposus for IVD regeneration	26
A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines	25
Automated classification of urine biomarkers to diagnose pancreatic cancer using 1-D convolutional neural networks	24
3D bioprinting of microfluidic systems for cardiac regenerative medicine: from biofabrication to organ-on-a-chip	24
Tunable PEGylation platform for efficient siRNA delivery using mesoporous silica nanoparticles	24
Enhancing chemical and physical stability of pharmaceuticals using freeze-thaw method: challenges and opportunities for process optimization through quality by design approach	23
Harnessing plant chloroplasts for oral delivery of a multi-epitope HPV vaccine: toward cost-effective systemic and mucosal immunization	23
Influence of shear force on ex vivo expansion of hematopoietic model cells in a stirred tank bioreactor	23
Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli	23
Chitosan modified with PAP as a promising delivery system for melatonin in the treatment of osteoporosis: targeting the divalent metal transporter 1	22
UniSePT: an engineered broadly applicable Unique Secretory signal PepTide for high-yield biotherapeutic production in mammalian cell-based expression systems	22
Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow	22
Nonthermal biocompatible plasma in stimulating osteogenic differentiation by targeting p38/ FOXO1 and PI3K/AKT pathways in hBMSCs	22
Exploring of therapeutic potential of indirubin-loaded nanofibrous scaffolds for localized melanoma treatment	21
Microfluidics-based cell recognition through optimizing suspended cell staining techniques and artificial intelligence	21
Fabricating a robust POSS-PCL nanofiber scaffold for nesting of mesenchymal stem cells: potential application in bone tissue regeneration	21
DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines	21
Early bioprocess development using Komagataella phaffii: application scenarios for the microTOM mini bioreactor system	20
Engineered Metal-Organic Frameworks (MOFs)-electrospun nanofibers for wound healing: a review	20
Thiol release improvement in Saccharomyces cerevisiae oenological strains by CRISPR/Cas9-based IRC7 molecular cisgenesis	20
Host cell protein impurities in therapeutic proteins: overview of advances in detection, nonconventional removal technologies and immunogenicity assessment	20
The lyophilized chloroplasts store synthetic DARPin G3 as bioactive encapsulated organelles	19
Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model	19
Biology-inspired engineering for circular bioeconomy systems	19
A pH-responsive ZnO2@ZIF-8/TCPP nanoplatform with self-supplied oxygen for hypoxia-relieved photodynamic therapy	19
Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms	18
Role of viscoelasticity in the appearance of low-Reynolds turbulence: considerations for modelling	18
High-throughput proliferation and activation of NK-92MI cell spheroids via a homemade one-step closed bioreactor in pseudostatic cultures for immunocellular therapy	18
An in vitro culture platform for studying the effect of collective cell migration on spatial self-organization within induced pluripotent stem cell colonies	18
Smartphone-based multispectral autofluorescence analysis of bacteria mixtures of staphylococci using convolutional neural network	18
Engineered chitosan/HP-β-CD hydrogel for targeted co-delivery of curcumin and berberine for the treatment of ulcerative colitis	18
Design and performance analysis of bionic trabecular bone scaffold structure based on Voronoi	18
Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid	17
Unraveling pathogenesis, biomarkers and potential therapeutic agents for endometriosis associated with disulfidptosis based on bioinformatics analysis, machine learning and experiment validation	17
Biomaterial-based chitosan nanohydrogel films: combination of Bistorta officinalis and Ca-doped carbon dots for improved blood clotting	16
Beyond linearity: reimagining AI as a participant in circular bioeconomies	16
Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology	16
Biomaterials for fetal membrane repair in preterm premature rupture of membranes: advances in tissue engineering strategies	16
Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox	16
CRISPRi-assisted metabolic engineering of cyanobacteria for photosynthetic hyaluronic acid from CO2	15
Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management	15
Advances in biotechnological methods for genetic and metabolic engineering in Methylomonas sp. DH-1	15
Clinical utility of mesenchymal stem/stromal cells in regenerative medicine and cellular therapy	15
Modular plug-and-play engineering of Klebsiella phages with dual receptor-binding proteins for efficient host range design	15
Effect of recombinant protein production and release on microalgal fitness and the impact of environmental conditions for localized therapeutic delivery	15
Therapeutic efficacy of mesenchymal stem cell-derived extracellular vesicles combined with injectable hydrogels in bone defect repair: a systematic review and meta-analysis of preclinical studies	14
Comparative analysis of heterologous lactate dehydrogenase expression in Ogataea polymorpha for enhanced L-Lactic acid production	14

Dual-responsive synthetic gene circuit for dynamic biologic drug delivery via inflammatory and circadian signaling pathways	14
Eggshell waste bioprocessing for sustainable acid phosphatase production and minimizing environmental hazards	14
Early introduction of 3D modeling modules promotes the development of simulation skills in downstream biomedical engineering curricula	14
Codon usage modulates the relationship between the burden and yield of protein overexpression	14
Quantitative impedance-based characterization of breast cancer cell migration and metastatic potential	14
Customized biofilm device for antibiofilm and antibacterial screening of newly developed nanostructured silver and zinc coatings	13
Enhanced methanol tolerance and utilization in Pichia pastoris revealed by adaptive laboratory evolution	13
Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures	13
Engineering probiotic Escherichia coli for inflammation-responsive indoleacetic acid production using RiboJ-enhanced genetic circuits	13
Efflux pump knockout minimizes crosstalk and boosts response in transcription factor-based biosensors	13
NIR-activated catechol-functionalized nanodiamond nanofibers for accelerating on-demand MRSA and E. coli biofilm eradication	13
Two-color spheroid model for determining the O2-induced radiosensitivity of HNSCC	12
Bakuchiol-loaded amphiphilic polymeric nanoparticles: enhancing stability and multifunctionality through encapsulation	12
Correction to: Challenges and progress of neurodrug: bioactivities, production and delivery strategies of nerve growth factor protein	12
From polyclonal to monoclonal: de novo sequencing of goat antibodies for a standardized ApoA-I immunoturbidimetric assay	12
A dual-inducible control system for multistep biosynthetic pathways	12
Engineering a conditionally active cetuximab prodrug via affibody-based paratope masking	12
High-titer 2-phenylethanol production in Escherichia coli via decarboxylation-step optimization and a vegetable-oil overlay for biocompatible in situ recovery	12
Fluorescent tools for the standardized work in Gram-negative bacteria	12
Combined drugs-loaded nano-lipodisk carrier for pH-responsive delivery for improved therapeutic potential on lung cancer model	12
Applications of carbon dots and its modified carbon dots in bone defect repair	12
Chitin nanowhiskers/octacalcium phosphate synergistically reinforced chitosan hydrogel for enhanced bone regeneration	11
Residual-hybrid dynamic response-surface/machine-learning framework for predicting malachite green removal and biodegradation by a bacterial consortium	11
Enhancing osteogenic differentiation of dental pulp stem cells through rosuvastatin loaded niosomes optimized by Box-Behnken design and modified by hyaluronan: a novel strategy for improved efficiency	11
Seaweed-based alginate/hydroxyapatite composite for the effective removal of bacteria, cyanobacteria, algae, and crystal violet from water	11
Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel	11
A novel inductively coupled capacitor wireless sensor system for rapid antibiotic susceptibility testing	11
Application of extracellular vesicles in the CRISPR-based diagnosis and treatment: possibilities and challenges	11
Anti-inflammatory activity of magnetic fields emitted by graphene devices on cultured human cells	10
Exosome-functionalized collagen-coated 3D-printed PCL scaffold for enhanced osteogenic differentiation and bone regeneration: an in vitro and in vivo study	10
Elucidating the metabolic landscape of Bacillus velezensis WY-65 in basic and cocktail nutrient media: a biocontrol approach against peanut web blotch	10
Insect cell expression system: advances in applications, engineering strategies, and bioprocess development	10
Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging	10
3D printed zirconia used as dental materials: a critical review	10
Phytoconstituents of Withania somnifera (L.) Dunal (Ashwagandha) unveiled potential cerebroside sulfotransferase inhibitors: insight through virtual screening, molecular dynamics, toxicity, and revers	10
Enhancing bioprocessing of red pigment from immobilized culture of gamma rays mutant of the endophytic fungus Monascus ruber SRZ112	10
Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units	10
Exosome-loaded hydrogels for bone regeneration: a cell-free therapeutic strategy	10
Engineering compact bacterial combinatorial promoters for two-input chemical AND switching	10
Correction: Follicular reconstruction and neo-oogenesis in xenotransplantation of human ovarian isolated cells derived from chemotherapy-induced POF patients	10
Smart colorimetric wound dressing for real-time infection detection and monitoring	10
Chitosan as a promising materials for the construction of nanocarriers for diabetic retinopathy: an updated review	10
Enhancing in vitro osteogenic differentiation of mesenchymal stem cells via sustained dexamethasone delivery in 3D-Printed hybrid scaffolds based on polycaprolactone-nanohydroxyapatite/alginate-gelati	10