OOIR: Observatory of International Research

Papers

(The median citation count of Journal of Biological Engineering is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-06-01 to 2025-06-01.)

Article	Citations
Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast	228
Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity	102
Gene syntaxes modulate gene expression and circuit behavior on plasmids	80
Cerium-doped calcium carbonate microparticles combined with low-intensity ultrasound for efficient sonodynamic therapy in body sculpting	69
Promoting osteogenesis and bone regeneration employing icariin-loaded nanoplatforms	61
Osteogenic differentiation of adipose-derived stem cells on dihydroartemisinin electrospun nanofibers	53
3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects	49
Tissue engineering of the gastrointestinal tract: the historic path to translation	38
A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy	35
Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo	34
3D-printed nanohydroxyapatite/methylacrylylated silk fibroin scaffold for repairing rat skull defects	31
Cell-cultivated aquatic food products: emerging production systems for seafood	27
Core-shell nanofiber dressings with zinc oxide nanoparticles and cell-free fat extract: boosting fibroblast activity and antibacterial efficacy	27
Research progress of biomimetic materials in oral medicine	26
Role of mesenchymal stem cell-derived exosomes in the regeneration of different tissues	25
A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines	25
Tissue engineering in age-related macular degeneration: a mini-review	24
Codeine 3-O-demethylase catalyzed biotransformation of morphinan alkaloids in Escherichia coli: site directed mutagenesis of terminal residues improves enzyme expression, stability and biotransformati	23
RN-Autoencoder: Reduced Noise Autoencoder for classifying imbalanced cancer genomic data	23
Quantitative imaging of magnetic nanoparticles in an unshielded environment using a large AC susceptibility array	23
Bio-engineered thermo-sensitive alginate/PNIA-g-CS co-polymeric injectable hydrogel laden with GDF-5 to stimulate nucleus pulposus for IVD regeneration	22
Influence of shear force on ex vivo expansion of hematopoietic model cells in a stirred tank bioreactor	21
Enhancing chemical and physical stability of pharmaceuticals using freeze-thaw method: challenges and opportunities for process optimization through quality by design approach	18
DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines	17
Automated classification of urine biomarkers to diagnose pancreatic cancer using 1-D convolutional neural networks	17

Nonthermal biocompatible plasma in stimulating osteogenic differentiation by targeting p38/ FOXO1 and PI3K/AKT pathways in hBMSCs	17
Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow	17
Fabricating a robust POSS-PCL nanofiber scaffold for nesting of mesenchymal stem cells: potential application in bone tissue regeneration	16
Chitosan modified with PAP as a promising delivery system for melatonin in the treatment of osteoporosis: targeting the divalent metal transporter 1	16
An in vitro culture platform for studying the effect of collective cell migration on spatial self-organization within induced pluripotent stem cell colonies	15
The use of a novel deer antler decellularized cartilage-derived matrix scaffold for repair of osteochondral defects	15
The lyophilized chloroplasts store synthetic DARPin G3 as bioactive encapsulated organelles	14
High-throughput proliferation and activation of NK-92MI cell spheroids via a homemade one-step closed bioreactor in pseudostatic cultures for immunocellular therapy	14
Rapid remodeling observed at mid-term in-vivo study of a smart reinforced acellular vascular graft implanted on a rat model	13
Identification and verification of diagnostic biomarkers for deep infiltrating endometriosis based on machine learning algorithms	13
Role of viscoelasticity in the appearance of low-Reynolds turbulence: considerations for modelling	13
Unraveling pathogenesis, biomarkers and potential therapeutic agents for endometriosis associated with disulfidptosis based on bioinformatics analysis, machine learning and experiment validation	13
Microfluidic chip and isothermal amplification technologies for the detection of pathogenic nucleic acid	12
Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox	12
Application of decellularized bone matrix as a bioscaffold in bone tissue engineering	12
Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology	12
Structural insights into inhibition of PRRSV Nsp4 revealed by structure-based virtual screening, molecular dynamics, and MM-PBSA studies	11
Fetal ischemia monitoring with in vivo implanted electrochemical multiparametric microsensors	11
Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management	11
CRISPRi-assisted metabolic engineering of cyanobacteria for photosynthetic hyaluronic acid from CO2	11
Clinical utility of mesenchymal stem/stromal cells in regenerative medicine and cellular therapy	11
Customized biofilm device for antibiofilm and antibacterial screening of newly developed nanostructured silver and zinc coatings	11
Dual-responsive synthetic gene circuit for dynamic biologic drug delivery via inflammatory and circadian signaling pathways	11
Biomaterial-based chitosan nanohydrogel films: combination of Bistorta officinalis and Ca-doped carbon dots for improved blood clotting	11
Early introduction of 3D modeling modules promotes the development of simulation skills in downstream biomedical engineering curricula	11
Eggshell waste bioprocessing for sustainable acid phosphatase production and minimizing environmental hazards	11
Potential application of hydrogel to the diagnosis and treatment of multiple sclerosis	11
NIR-activated catechol-functionalized nanodiamond nanofibers for accelerating on-demand MRSA and E. coli biofilm eradication	10
Correction: Engineered hypoxia-responding Escherichia coli carrying cardiac peptide genes, suppresses tumor growth, angiogenesis and metastasis in vivo	10
Codon usage modulates the relationship between the burden and yield of protein overexpression	10
Fluorescent tools for the standardized work in Gram-negative bacteria	10
Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases	10
Engineering probiotic Escherichia coli for inflammation-responsive indoleacetic acid production using RiboJ-enhanced genetic circuits	10
Review: biological engineering for nature-based climate solutions	10
Microfluidic device for enhancement and analysis of osteoblast differentiation in three-dimensional cell cultures	10
Applications of carbon dots and its modified carbon dots in bone defect repair	9
Seaweed-based alginate/hydroxyapatite composite for the effective removal of bacteria, cyanobacteria, algae, and crystal violet from water	9
Enhancing bioprocessing of red pigment from immobilized culture of gamma rays mutant of the endophytic fungus Monascus ruber SRZ112	9
Phytoconstituents of Withania somnifera (L.) Dunal (Ashwagandha) unveiled potential cerebroside sulfotransferase inhibitors: insight through virtual screening, molecular dynamics, toxicity, and revers	9
A novel inductively coupled capacitor wireless sensor system for rapid antibiotic susceptibility testing	9
Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel	8
3D printed zirconia used as dental materials: a critical review	8
Anti-inflammatory activity of magnetic fields emitted by graphene devices on cultured human cells	8
A dual-inducible control system for multistep biosynthetic pathways	8
Correction: Follicular reconstruction and neo-oogenesis in xenotransplantation of human ovarian isolated cells derived from chemotherapy-induced POF patients	8
Enhancing osteogenic differentiation of dental pulp stem cells through rosuvastatin loaded niosomes optimized by Box-Behnken design and modified by hyaluronan: a novel strategy for improved efficiency	8
Light regulation of secondary metabolism in fungi	7
Enhancing in vitro osteogenic differentiation of mesenchymal stem cells via sustained dexamethasone delivery in 3D-Printed hybrid scaffolds based on polycaprolactone-nanohydroxyapatite/alginate-gelati	7
Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors	7
Chitosan as a promising materials for the construction of nanocarriers for diabetic retinopathy: an updated review	7

Soil microbiome characterization and its future directions with biosensing	7
Optimizing the fabrication of a 3D high-resolution implant for neural stimulation	7
Challenges of aortic valve tissue culture – maintenance of viability and extracellular matrix in the pulsatile dynamic microphysiological system	6
Marimo actuated rover systems	6
A novel computationally engineered collagenase reduces the force required for tooth extraction in an ex-situ porcine jaw model	6
Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units	6
Unravelling the mechanotransduction pathways in Alzheimer’s disease	6
Non-spherical gold nanoparticles enhanced fluorescence of carbon dots for norovirus-like particles detection	6
An engineered ligand-responsive Csy4 endoribonuclease controls transgene expression from Sendai virus vectors	6
Knockdown of NCK1-AS1 inhibits the development of atherosclerosis by targeting miR-1197/COX10 axis	6
Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology	5
High throughput mutagenesis and screening for yeast engineering	5
An electrochemical biosensor for the detection of microRNA-31 as a potential oral cancer biomarker	5
Correction to: Exosomes derived from microRNA-138-5poverexpressing bone marrow-derived mesenchymal stem cells confer neuroprotection to astrocytes following ischemic stroke via inhibition of LCN2	5
Mixed culture biocatalytic production of the high-value biochemical 7-methylxanthine	5
Advances in the application of gas vesicles in medical imaging and disease treatment	5
Metabolic engineering of Escherichia coli BW25113 for the production of 5-Aminolevulinic Acid based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification	5
Multilayered blow-spun vascular prostheses with luminal surfaces in Nano/Micro range: the influence on endothelial cell and platelet adhesion	5
Gentamicin-loaded chitosan/folic acid-based carbon quantum dots nanocomposite hydrogel films as potential antimicrobial wound dressing	5
Assessment of the efficiency of synergistic photocatalysis on penicillin G biodegradation by whole cell Paracoccus sp	5
Label-free multimodal electro-thermo-mechanical (ETM) phenotyping as a novel biomarker to differentiate between normal, benign, and cancerous breast biopsy tissues	5
Hypoxia and re-oxygenation effects on human cardiomyocytes cultured on polycaprolactone and polyurethane nanofibrous mats	5
Development of a novel defined minimal medium for Gluconobacter oxydans 621H by systematic investigation of metabolic demands	5
Breast cancer brain metastasis: from etiology to state-of-the-art modeling	5
The progressive trend of modeling and drug screening systems of breast cancer bone metastasis	5
High-throughput strategies for monoclonal antibody screening: advances and challenges	5
Surface modification of decellularized bovine carotid arteries with human vascular cells significantly reduces their thrombogenicity	5
Epithelial cell-cell interactions in an overcrowded environment: jamming or live cell extrusion	5
Exosomes derived from heat shock preconditioned bone marrow mesenchymal stem cells alleviate cisplatin-induced ototoxicity in mice	5
Metabolic engineering of Saccharomyces cerevisiae for the biosynthesis of a fungal pigment from the phytopathogenic fungus Cladosporium phlei	4
Novel organoid construction strategy for non-involuting congenital hemangioma for drug validation	4
Advanced biomanufacturing and evaluation of adeno-associated virus	4
Serum-free endothelial cell culture medium for vascular smooth muscle cells sheet formation	4
Biomolecular and cellular effects in skin wound healing: the association between ascorbic acid and hypoxia-induced factor	4
Reversible fluorescent solid porous films for detection of zinc ions in biological media	4
Targeting the PEDV 3CL protease for identification of small molecule inhibitors: an insight from virtual screening, ADMET prediction, molecular dynamics, free energy landscape, and binding energy calc	4
From microbial proteins to cultivated meat for alternative meat-like products: a review on sustainable fermentation approaches	4
Exosome-mediated crosstalk between epithelial cells amplifies the cell injury cascade in CaOx stone formation	4
Scale-up of CHO cell cultures: from 96-well-microtiter plates to stirred tank reactors across three orders of magnitude	4
Exploring the impact of taurine on the biochemical properties of urate oxidase: response surface methodology and molecular dynamics simulation	4
Proteomic analysis of decellularized mice liver and kidney extracellular matrices	4
Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions	4
Hyperactivation of crosslinked lipases in elastic hydroxyapatite microgel and their properties	4
Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol	3
Optimize the parameters for the synthesis by the ionic gelation technique, purification, and freeze-drying of chitosan-sodium tripolyphosphate nanoparticles for biomedical purposes	3
PLGA/BGP/Nef porous composite restrains osteoclasts by inhibiting the NF-κB pathway, enhances IGF-1-mediated osteogenic differentiation and promotes bone regeneration	3
Treatment of effluents from Food Services Establishment (FSEs) by Physico-chemical processes: a case study for Trinidad & Tobago	3
Dual-drug (Curcumin/Ciprofloxacin) loading and release from chitosan-based hydrogels embedded with magnetic Montmorillonite/Hyaluronic acid for enhancing wound healing	3
CRISPR/Cas12a-mediated gene silencing across diverse functional genes demonstrates single gene-specific spacer efficacy in Mycobacterium smegmatis	3
Mussel-inspired electroactive, antibacterial and antioxidative composite membranes with incorporation of gold nanoparticles and antibacterial peptides for enhancing skin wound healing	3
Investigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis	3
Evaluating ATP testing for distribution system monitoring: comparison to HPC, impact of chlorine quenching, and hold time dependency	3
Nucleic acid direct delivery to fibroblasts: a review of nucleofection and applications	3
Termination sequence between an inducible promoter and ubiquitous chromatin opening element (UCOE) reduces gene expression leakage and silencing	3
Synthesizing hybrid copper phosphate (Cu3(PO4)2) nanoflowers using Cu+2 and shed snakeskin: antioxidant, antibacterial, anticancer, guaiacol, anionic, and cationic dye degradation properties	3
Preparation of bilayer tissue-engineered polyurethane/poly-L-lactic acid nerve conduits and their in vitro characterization for use in peripheral nerve regeneration	3