Plant Methods

Article	Citations
VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells	496
Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge	85
Protocol: a simple method for biosensor visualization of bacterial quorum sensing and quorum quenching interaction on Medicago roots	79
Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum	77
The influence of water content on the longitudinal modulus of elasticity of maize stalk pith and rind tissues	59
Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying	57
A device for the controlled cooling and freezing of excised plant specimens during magnetic resonance imaging	55
Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves	54
A robust and efficient automatic method to segment maize FASGA stained stem cross section images to accurately quantify histological profile	51
Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR	50
Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors	48
Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development	43
Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two	37
Stability investigation of air-dried olive ribo nucleic acids for metavirome studies	37
Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry	36
Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis	36
Electrical impedance spectroscopy (EIS) in plant roots research: a review	36
Efficient transformation of the isolated microspores of Chinese cabbage (Brassica rapa L. ssp. pekinensis) by particle bombardment	35
The semi-automated development of plant cell wall finite element models	35
Semi-supervised few-shot learning approach for plant diseases recognition	35
Micro computed tomography analysis of barley during the first 24 hours of germination	34
A novel selection model based on multivariate methods and arbitrary genetic parameters: a case study on tomato families	33
Rapid identification of mutations caused by fast neutron bombardment in Medicago truncatula	32
Optimizing PGRs for in vitro shoot proliferation of pomegranate with bayesian-tuned ensemble stacking regression and NSGA-II: a comparative evaluation of machine learning models	32
A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit	32

An automated method for stem diameter measurement based on laser module and deep learning	31
Methods to optimize optical sensing of biotic plant stress – combined effects of hyperspectral imaging at night and spatial binning	31
Karst vegetation coverage detection using UAV multispectral vegetation indices and machine learning algorithm	30
Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken	30
Identifying and mapping individual medicinal plant Lamiophlomis rotata at high elevations by using unmanned aerial vehicles and deep learning	28
Improved estimation of aboveground biomass of regional coniferous forests integrating UAV-LiDAR strip data, Sentinel-1 and Sentinel-2 imageries	27
The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma	26
Optimized synthesis of layered double hydroxide lactate nanosheets and their biological effects on Arabidopsis seedlings	26
AprGPD: the apricot genomic and phenotypic database	26
High-throughput phenotyping allows the selection of soybean genotypes for earliness and high grain yield	25
Characterizing the effects of different chemicals on stem bending of cut snapdragon flower	25
A novel visual marker to distinguish haploids from doubled haploids in rice (Oryza sativa, L) at early growth stages	24
Combining Fourier-transform infrared spectroscopy and multivariate analysis for chemotyping of cell wall composition in Mungbean (Vigna radiata (L.) Wizcek)	23
A systematic high-throughput phenotyping assay for sugarcane stalk quality characterization by near-infrared spectroscopy	23
Improved genomic prediction using machine learning with Variational Bayesian sparsity	23
Orchid2024: A cultivar-level dataset and methodology for fine-grained classification of Chinese Cymbidium Orchids	23
One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells	23
TreeJ: an ImageJ plugin for interactive cell lineage reconstruction from static images	23
Soybean leaf estimation based on RGB images and machine learning methods	22
An efficient screening system of disease-resistant genes from wild apple, Malus sieversii in response to Valsa mali pathogenic fungus	22
Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology	22
Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS	22
Advanced screening methods for assessing motility and hatching in plant-parasitic nematodes	22
De novo assembly provides new insights into the evolution of Elaeagnus angustifolia L.	21
Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids	21
Efficient isolation and purification of tissue-specific protoplasts from tea plants (Camellia sinensis (L.) O. Kuntze)	19
Application of machine learning algorithms and feature selection in rapeseed (Brassica napus L.) breeding for seed yield	19
Hyperspectral imaging-based classification of rice leaf blast severity over multiple growth stages	19
Systematic investigation and validation of peanut genetic transformation via the pollen tube injection method	18
Improving the prediction performance of leaf water content by coupling multi-source data with machine learning in rice (Oryza sativa L.)	18
Temporal resolution trumps spectral resolution in UAV-based monitoring of cereal senescence dynamics	18
A novel method for extraction of high purity and high production Phytophthora sojae oospores	18
Metric learning for image-based flower cultivars identification	18
Corn360: a method for quantification of corn kernels	17
Segmentation and counting of wheat spike grains based on deep learning and textural feature	17
Computer vision-based wood identification and its expansion and contribution potentials in wood science: A review	17
A deep learning approach to track Arabidopsis seedlings’ circumnutation from time-lapse videos	17
A handheld device for measuring the diameter at breast height of individual trees using laser ranging and deep-learning based image recognition	17
A label-free, fast and high-specificity technique for plant cell wall imaging and composition analysis	17
Non-destructive real-time monitoring of underground root development with distributed fiber optic sensing	17
Versatile DNA extraction from diverse plant taxa using ionic liquids and magnetic ionic liquids: a methodological breakthrough for enhanced sample utility	16
A method to experimentally clamp leaf water content to defined values to assess its effects on apoplastic pH	16
Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE	15
In vivo characterisation of field pea stem wall thickness using optical coherence tomography	15
Fast estimation of plant growth dynamics using deep neural networks	15
Roughness measurement of leaf surface based on shape from focus	15
An efficient grafting method for phytoplasma transmission in Catharanthus roseus	15
Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study	15
Phenotyping seedlings for selection of root system architecture in alfalfa (Medicago sativa L.)	15
Estimation of cotton canopy parameters based on unmanned aerial vehicle (UAV) oblique photography	15

A CNN model for early detection of pepper Phytophthora blight using multispectral imaging, integrating spectral and textural information	15
A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction	15
Ripening dynamics revisited: an automated method to track the development of asynchronous berries on time-lapse images	14
A random-sampling approach to track cell divisions in time-lapse fluorescence microscopy	14
Early detection of verticillium wilt in eggplant leaves by fusing five image channels: a deep learning approach	14
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean	14
Applying spectral fractal dimension index to predict the SPAD value of rice leaves under bacterial blight disease stress	14
HairNet2: deep learning to quantify cotton leaf hairiness, a complex genetic and environmental trait	14
Prediction accuracy and repeatability of UAV based biomass estimation in wheat variety trials as affected by variable type, modelling strategy and sampling location	14
Can allele-specific loop-mediated isothermal amplification be used for rapid detection of target-site herbicide resistance in Lolium spp.?	13
Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry	13
Apnet: Lightweight network for apricot tree disease and pest detection in real-world complex backgrounds	13
Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts	13
Study on canopy extraction method for narrowband spectral images based on superpixel color gradation skewness distribution features	13
Classification of multi-year and multi-variety pumpkin seeds using hyperspectral imaging technology and three-dimensional convolutional neural network	13
A cassava common mosaic virus vector for virus-induced gene silencing in cassava	13
Automatic freezing-tolerant rapeseed material recognition using UAV images and deep learning	13
Tensor methods in data analysis of chromatography/mass spectroscopy-based plant metabolomics	12
A streamlined guide RNA screening system for genome editing in Sorghum bicolor	12
Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants	12
Identification of the initial water-site and movement in Gleditsia sinensis seeds and its relation to seed coat structure	12
Combining novel feature selection strategy and hyperspectral vegetation indices to predict crop yield	12
High-throughput phenotyping for non-destructive estimation of soybean fresh biomass using a machine learning model and temporal UAV data	11
A model for genuineness detection in genetically and phenotypically similar maize variety seeds based on hyperspectral imaging and machine learning	11
Correction: Revisiting superiority and stability metrics of cultivar performances using genomic data: derivations of new estimators	11
A comprehensive review of in planta stable transformation strategies	11
Determination of wheat spike and spikelet architecture and grain traits using X-ray Computed Tomography imaging	11
Small-scale sequencing enables quality assessment of Ribo-Seq data: an example from Arabidopsis cell culture	11
Leaf area index estimation model for UAV image hyperspectral data based on wavelength variable selection and machine learning methods	11
Correction: Maximizing efficiency in sunflower breeding through historical data optimization	11
Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry	11
Axial variation in flexural stiffness of plant stem segments: measurement methods and the influence of measurement uncertainty	11
Soybean cyst nematode detection and management: a review	11
Species-specific basecallers improve actual accuracy of nanopore sequencing in plants	11
A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]	11
Applying quantitative spatial phenotypes analysis to the investigation of peltate glandular trichomes development pattern in Perilla frutescens	10
Publisher Correction: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i	10
Hyperspectral imaging for pest symptom detection in bell pepper	10
PhenoTrack3D: an automatic high-throughput phenotyping pipeline to track maize organs over time	10
Correction: YOLO POD: a fast and accurate multi-task model for dense Soybean Pod counting	10
Atmospheric correction of vegetation reflectance with simulation-trained deep learning for ground-based hyperspectral remote sensing	10
DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular application	10
Terrestrial laser scanning and low magnetic field digitization yield similar architectural coarse root traits for 32-year-old Pinus ponderosa trees	10
Optimization of ribosome profiling in plants including structural analysis of rRNA fragments	10
Cucumber pathogenic spores’ detection using the GCS-YOLOv8 network with microscopic images in natural scenes	10
Rice bacterial blight resistant cultivar selection based on visible/near-infrared spectrum and deep learning	9
Evaporative flux method of leaf hydraulic conductance estimation: sources of uncertainty and reporting format recommendation	9
Exploring genetic variability under extended photoperiod in lentil (Lens Culinaris Medik): vegetative and phenological differentiation according to genetic material’s origins	9
Image analysis and polyphenol profiling unveil red-flesh apple phenotype complexity	9
Plant diseases and pests detection based on deep learning: a review	9
A procedure for Dex-induced gene transactivation in Arabidopsis ovules	9
Innovation of strip fertilization planting for rice straw crushing with back-throwing and interrow-laying	9
ShinyCore: An R/Shiny program for establishing core collection based on single nucleotide polymorphism data	9
Optimization of preparation and transformation of protoplasts from Populus simonii × P. nigra leaves and subcellular localization of the major latex protein 328 (MLP328)	9
Cryopreservation of Abies alba × A. numidica and Pinus nigra embryogenic tissues by stepwise dehydration method	9
Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture	9
Combination of long-term 13CO2 labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves	9
X-ray driven peanut trait estimation: computer vision aided agri-system transformation	9
Gray mold and anthracnose disease detection on strawberry leaves using hyperspectral imaging	9
A toolkit to rapidly modify root systems through single plant selection	9
Early real-time detection algorithm of tomato diseases and pests in the natural environment	9
Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol	9
Clarification of the infection pattern of Xanthomonas citri subsp. citri on citrus fruit by artificial inoculation	8
Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)	8
A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance	8
Meta-analysis of the role of zinc in coordinating absorption of mineral elements in wheat seedlings	8
A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum	8
Four-dimensional measurement of root system development using time-series three-dimensional volumetric data analysis by backward prediction	8
Semantic segmentation of plant roots from RGB (mini-) rhizotron images—generalisation potential and false positives of established methods and advanced deep-learning models	8
Effects of cushion box and closed let-down ladder usage on damage to corn during handling: physiological deterioration	8
Maize-IAS: a maize image analysis software using deep learning for high-throughput plant phenotyping	8
Texture feature extraction from microscope images enables a robust estimation of ER body phenotype in Arabidopsis	8
A novel in-situ-process technique constructs whole circular cpDNA library	8
Feature enhancement guided network for yield estimation of high-density jujube	8
K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology	8
A technical guide to TRITEX, a computational pipeline for chromosome-scale sequence assembly of plant genomes	8
An exploration of the influence of ZnO NPs treatment on germination of radish seeds under salt stress based on the YOLOv8-R lightweight model	8
Novel in silico screening system for plant defense activators using deep learning-based prediction of reactive oxygen species accumulation	7
RhizoMAP: a comprehensive, nondestructive, and sensitive platform for metabolic imaging of the rhizosphere	7
MGIDI: a powerful tool to analyze plant multivariate data	7

A pin-based probe for electronic moisture meters to determine moisture content in a single wheat kernel	7
Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants	7
Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques	7
Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis	7
Mechanism of self-recovery of hydrophobicity after surface damage of lotus leaf	7
Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH	7
Comparison of new hyperspectral index and machine learning models for prediction of winter wheat leaf water content	7
Correction: Soybean seed pest damage detection method based on spatial frequency domain imaging combined with RL-SVM	7
Segmentation and characterization of macerated fibers and vessels using deep learning	7
Improved wood species identification based on multi-view imagery of the three anatomical planes	7
‘Single-Seed-SpeedBulks:’ a protocol that combines ‘speed breeding’ with a cost-efficient modified single-seed descent method for rapid-generation-advancement in oat (Avena sativa L.)	7
Chemoinformatics-driven classification of Angiosperms using sulfur-containing compounds and machine learning algorithm	7
A comprehensive tool in recycling plant-waste of Gossypium barbadense L agricultural and industrial waste extracts containing gossypin and gossypol: hepatoprotective, anti-inflammatory and antioxidant	7
Deep learning for detecting herbicide weed control spectrum in turfgrass	7
Non-wounding contact-based Inoculation of fruits with fungal pathogens in postharvest	7
A global non-invasive methodology for the phenotyping of potato under water deficit conditions using imaging, physiological and molecular tools	7
Characterization of leaf surface phenotypes based on light interaction	7
High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing	7
Whole mount multiplexed visualization of DNA, mRNA, and protein in plant-parasitic nematodes	7
Computational fluid dynamics simulation analysis of the effect of curved rice leaves on the deposition behaviour of droplets	7
Wheat leaf area index prediction using data fusion based on high-resolution unmanned aerial vehicle imagery	7
An improved bacterial mRNA enrichment strategy in dual RNA sequencing to unveil the dynamics of plant-bacterial interactions	7