Plant Methods

Article	Citations
The influence of water content on the longitudinal modulus of elasticity of maize stalk pith and rind tissues	105
Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two	103
Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology	99
TreeJ: an ImageJ plugin for interactive cell lineage reconstruction from static images	74
Orchid2024: A cultivar-level dataset and methodology for fine-grained classification of Chinese Cymbidium Orchids	67
Stability investigation of air-dried olive ribo nucleic acids for metavirome studies	62
The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma	62
A novel method for extraction of high purity and high production Phytophthora sojae oospores	57
A deep learning approach to track Arabidopsis seedlings’ circumnutation from time-lapse videos	53
Classification of multi-year and multi-variety pumpkin seeds using hyperspectral imaging technology and three-dimensional convolutional neural network	52
Small-scale sequencing enables quality assessment of Ribo-Seq data: an example from Arabidopsis cell culture	50
An automated method for stem diameter measurement based on laser module and deep learning	47
The semi-automated development of plant cell wall finite element models	46
Optimized synthesis of layered double hydroxide lactate nanosheets and their biological effects on Arabidopsis seedlings	45
Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry	43
Non-destructive real-time monitoring of underground root development with distributed fiber optic sensing	43
Fast estimation of plant growth dynamics using deep neural networks	42
Estimation of cotton canopy parameters based on unmanned aerial vehicle (UAV) oblique photography	42
Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts	41
Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors	40
A simple and efficient method to quantify the cell parameters of the seed coat, embryo and silique wall in rapeseed	37
Electrical impedance spectroscopy (EIS) in plant roots research: a review	37
Meta-analysis of the role of zinc in coordinating absorption of mineral elements in wheat seedlings	36
Improved wood species identification based on multi-view imagery of the three anatomical planes	34
An improved bacterial mRNA enrichment strategy in dual RNA sequencing to unveil the dynamics of plant-bacterial interactions	33

RiboPlotR: a visualization tool for periodic Ribo-seq reads	32
Combination of long-term 13CO2 labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves	32
Innovation of strip fertilization planting for rice straw crushing with back-throwing and interrow-laying	32
High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing	30
Optimization of ribosome profiling in plants including structural analysis of rRNA fragments	29
Novel in silico screening system for plant defense activators using deep learning-based prediction of reactive oxygen species accumulation	29
Local mapping of root orientation traits by X-ray micro-CT and 3d image analysis: A study case on carrot seedlings grown in simulated vs real weightlessness	29
Synchrotron tomography applications in agriculture and food sciences research: a review	29
A statistical method for high-throughput emergence rate calculation for soybean breeding plots based on field phenotypic characteristics	28
A global non-invasive methodology for the phenotyping of potato under water deficit conditions using imaging, physiological and molecular tools	28
A method of two-dimensional correlation spectroscopy combined with residual neural network for comparison and differentiation of medicinal plants raw materials superior to traditional machine learning	28
ScAnalyzer: an image processing tool to monitor plant disease symptoms and pathogen spread in Arabidopsis thaliana leaves	27
Automated, image-based disease measurement for phenotyping resistance to soybean frogeye leaf spot	27
An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome	27
Machine learning provides specific detection of salt and drought stresses in cucumber based on miRNA characteristics	27
Inter-row navigation line detection for cotton with broken rows	27
Using near-infrared spectroscopy to predict nitrogen and phosphorus concentrations of herbarium specimens under different storage conditions	27
RGB image-based method for phenotyping rust disease progress in pea leaves using R	26
Automatic rape flower cluster counting method based on low-cost labelling and UAV-RGB images	26
GrainNet: efficient detection and counting of wheat grains based on an improved YOLOv7 modeling	24
Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin	24
Evaluation of deep learning for predicting rice traits using structural and single-nucleotide genomic variants	24
DWTFormer: a frequency-spatial features fusion model for tomato leaf disease identification	24
Assembly and operation of an imaging system for long-term monitoring of bioluminescent and fluorescent reporters in plants	23
A new and effective method to induce infection of Phyllachora maydis into corn for tar spot studies in controlled environments	23
A rapid and non-destructive method for spatial–temporal quantification of colonization by Pseudomonas syringae pv. tomato DC3000 in Arabidopsis and tomato	23
A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy	23
Improvement of germination rate and hybridization to facilitate breeding of an industrial oil crop, Euphorbia lagascae Spreng	22
A comparative study on point cloud down-sampling strategies for deep learning-based crop organ segmentation	22
Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling	21
Installation and imaging of thousands of minirhizotrons to phenotype root systems of field-grown plants	21
Evaluation of an intelligent artificial climate chamber for high-throughput crop phenotyping in wheat	21
Quantitative phenotyping of crop roots with spectral electrical impedance tomography: a rhizotron study with optimized measurement design	21
An electrophoretic mobility shift assay using the protein isolated from host plants	21
SYMPATHIQUE: image-based tracking of symptoms and monitoring of pathogenesis to decompose quantitative disease resistance in the field	21
BerryPortraits: Phenotyping Of Ripening Traits in cranberry (Vaccinium macrocarpon Ait.) with YOLOv8	20
In vitro micropropagation protocols for two endangered Dianthus species - via in vitro culture for conservation and recultivation purposes	20
Scoring the number of B chromosomes in Zea mays L. using droplet digital PCR assay	20
Spectral estimation of the aboveground biomass of cotton under water–nitrogen coupling conditions	20
Sorting biotic and abiotic stresses on wild rocket by leaf-image hyperspectral data mining with an artificial intelligence model	20
A compact deep learning approach integrating depthwise convolutions and spatial attention for plant disease classification	19
Statistical analysis of organelle movement using state-space models	19
WheatLFANet: in-field detection and counting of wheat heads with high-real-time global regression network	19
Estimation of plant height and yield based on UAV imagery in faba bean (Vicia faba L.)	18
Wheat physiology predictor: predicting physiological traits in wheat from hyperspectral reflectance measurements using deep learning	18
Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter	18
Refining bulk segregant analyses: ontology-mediated discovery of flowering time genes in Brassica oleracea	18
Detection and characterization of spike architecture based on deep learning and X-ray computed tomography in barley	18
High-throughput soybean seeds phenotyping with convolutional neural networks and transfer learning	18
Insights into lncRNA-mediated regulatory networks in Hevea brasiliensis under anthracnose stress	17

A hybrid CNN–SVM classifier for weed recognition in winter rape field	17
A method to determine antifungal activity in seed exudates by nephelometry	17
Assessing data analysis techniques in a high-throughput meiosis-like induction detection system	17
Optimizing cannabis cultivation: an efficient in vitro system for flowering induction	16
A custom library construction method for super-resolution ribosome profiling in Arabidopsis	16
Acceleration of wheat breeding: enhancing efficiency and practical application of the speed breeding system	16
Spatial and Texture Analysis of Root System distribution with Earth mover’s Distance (STARSEED)	16
Automating high-throughput screening for anthracnose resistance in common bean using allele specific PCR	16
Pre-trained protein language model sheds new light on the prediction of Arabidopsis protein–protein interactions	16
Transmembrane potential, an indicator in situ reporting cellular senescence and stress response in plant tissues	16
Intelligent identification on cotton verticillium wilt based on spectral and image feature fusion	16
Mitochondrial DNA editing in potato through mitoTALEN and mitoTALECD: molecular characterization and stability of editing events	16
A high-throughput approach for quantifying turgor loss point in grapevine	15
Efficient mutagenesis and genotyping of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR	15
A deep learning approach for deriving wheat phenology from near-surface RGB image series using spatiotemporal fusion	15
A vector-free gene interference system using delaminated Mg–Al-lactate layered double hydroxide nanosheets as molecular carriers to intact plant cells	15
Monitoring of plant water uptake by measuring root dielectric properties on a fine timescale: diurnal changes and response to leaf excision	15
An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues	15
Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy	15
Genotyping of polyploid plants using quantitative PCR: application in the breeding of white-fleshed triploid loquats (Eriobotrya japonica)	15
DeepCob: precise and high-throughput analysis of maize cob geometry using deep learning with an application in genebank phenomics	15
A practical guide to estimating the light extinction coefficient with nonlinear models—a case study on maize	15
Mapping and quantifying unique branching structures in lentil (Lens culinaris Medik.)	14
Characterizing the effects of different chemicals on stem bending of cut snapdragon flower	14
Improved genomic prediction using machine learning with Variational Bayesian sparsity	14
A CNN model for early detection of pepper Phytophthora blight using multispectral imaging, integrating spectral and textural information	14
AraDQ: an automated digital phenotyping software for quantifying disease symptoms of flood-inoculated Arabidopsis seedlings	14
Sensor-based phenotyping of above-ground plant-pathogen interactions	14
Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken	14
Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants	14
Accelerating eucalypt clone selection pipeline via cloned progeny trials and molecular data	14
Low-cost and reliable substrate-based phenotyping platform for screening salt tolerance of cutting propagation-dependent grass, paspalum vaginatum	14
BioCurve Analyzer: a web-based shiny app for analyzing biological response curves	14
De novo assembly provides new insights into the evolution of Elaeagnus angustifolia L.	14
A novel selection model based on multivariate methods and arbitrary genetic parameters: a case study on tomato families	14
Determining water status of walnut orchards using the crop water stress index and canopy temperature measurements	14
Eggsplorer: a rapid plant–insect resistance determination tool using an automated whitefly egg quantification algorithm	14
One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells	13
Apnet: Lightweight network for apricot tree disease and pest detection in real-world complex backgrounds	13
A cassava common mosaic virus vector for virus-induced gene silencing in cassava	13
Advancing radiation-induced mutant screening through high-throughput technology: a preliminary evaluation of mutant screening in Arabidopsis thaliana	13
Publisher Correction: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i	13
Identifying and mapping individual medicinal plant Lamiophlomis rotata at high elevations by using unmanned aerial vehicles and deep learning	13
Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids	12
Metric learning for image-based flower cultivars identification	12
Clarification of the infection pattern of Xanthomonas citri subsp. citri on citrus fruit by artificial inoculation	12
RhizoMAP: a comprehensive, nondestructive, and sensitive platform for metabolic imaging of the rhizosphere	12
A cell P system with membrane division and dissolution rules for soybean leaf disease recognition	12
Combining novel feature selection strategy and hyperspectral vegetation indices to predict crop yield	12
A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction	12
A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: suitable for large-scale assessment of resistance in poplar breeding progeny	12
Correction: Rapid and cost-effective molecular karyotyping in wheat, barley, and their crossprogeny by chromosome-specific multiplex PCR	12
ShinyCore: An R/Shiny program for establishing core collection based on single nucleotide polymorphism data	12
Phenotyping seedlings for selection of root system architecture in alfalfa (Medicago sativa L.)	12
Segmentation and counting of wheat spike grains based on deep learning and textural feature	12
Non-wounding contact-based Inoculation of fruits with fungal pathogens in postharvest	12
Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue	12
Deep learning for detecting herbicide weed control spectrum in turfgrass	11
Establishment of an NPK nutrient monitor system in yield-graded cotton petioles under drip irrigation	11
Quality assurance of hyperspectral imaging systems for neural network supported plant phenotyping	11
Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture	11
A novel approach integrating multispectral imaging and machine learning to identify seed maturity and vigor in smooth bromegrass	11
Gray mold and anthracnose disease detection on strawberry leaves using hyperspectral imaging	11
In-depth evaluation of root infection systems using the vascular fungus Verticillium longisporum as soil-borne model pathogen	11
Rapid real-time quantitative colorimetric LAMP methodology for field detection of Verticillium dahliae in crude olive-plant samples	11
Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings	11
PhenoTrack3D: an automatic high-throughput phenotyping pipeline to track maize organs over time	11
Dual discriminator GAN-based synthetic crop disease image generation for precise crop disease identification	11
Characterization of leaf surface phenotypes based on light interaction	11
Accelerating leaf area measurement using a volumetric approach	11
Overexpressing Arabidopsis thaliana ACBP6 in transgenic rapid-cycling Brassica napus confers cold tolerance	11
Yield estimation of high-density cotton fields using low-altitude UAV imaging and deep learning	11
DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular application	11
Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants	11
The mechanisms and prediction of non-structural carbohydrates accretion and depletion after mechanical wounding in slash pine (Pinus elliottii) using near-infrared reflectance spectroscopy	10
Soybean seed pest damage detection method based on spatial frequency domain imaging combined with RL-SVM	10
Swin-Unet++: a study on phenotypic parameter analysis of cabbage seedling roots	10
A CNN-LSTM-att hybrid model for classification and evaluation of growth status under drought and heat stress in chinese fir (Cunninghamia lanceolata)	10
High-resolution spectral information enables phenotyping of leaf epicuticular wax in wheat	10
In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots	10

Opening the black box: explainable deep-learning classification of wood microscopic image of endangered tree species	10
An automated phenotyping method for Chinese Cymbidium seedlings based on 3D point cloud	10
Photolysis of caged cytokinin in single cells of Arabidopsis thaliana	10
Developing non-invasive 3D quantificational imaging for intelligent coconut analysis system with X-ray	10
Exploring the impact of light intensity under speed breeding conditions on the development and growth of lentil and chickpea	10
Segmentation of structural parts of rosebush plants with 3D point-based deep learning methods	10