Plant Methods

Papers
(The median citation count of Plant Methods is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-02-01 to 2025-02-01.)
ArticleCitations
VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells496
Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge85
Protocol: a simple method for biosensor visualization of bacterial quorum sensing and quorum quenching interaction on Medicago roots79
Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum77
The influence of water content on the longitudinal modulus of elasticity of maize stalk pith and rind tissues59
Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying57
A device for the controlled cooling and freezing of excised plant specimens during magnetic resonance imaging55
Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves54
A robust and efficient automatic method to segment maize FASGA stained stem cross section images to accurately quantify histological profile51
Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR50
Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors48
Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development43
Stability investigation of air-dried olive ribo nucleic acids for metavirome studies37
Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two37
Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis36
Electrical impedance spectroscopy (EIS) in plant roots research: a review36
Profiling of 1-aminocyclopropane-1-carboxylic acid and selected phytohormones in Arabidopsis using liquid chromatography-tandem mass spectrometry36
The semi-automated development of plant cell wall finite element models35
Semi-supervised few-shot learning approach for plant diseases recognition35
Efficient transformation of the isolated microspores of Chinese cabbage (Brassica rapa L. ssp. pekinensis) by particle bombardment35
Micro computed tomography analysis of barley during the first 24 hours of germination34
A novel selection model based on multivariate methods and arbitrary genetic parameters: a case study on tomato families33
Optimizing PGRs for in vitro shoot proliferation of pomegranate with bayesian-tuned ensemble stacking regression and NSGA-II: a comparative evaluation of machine learning models32
A quantitative method to measure geranylgeranyl diphosphate (GGPP) and geranylgeranyl monophosphate (GGP) in tomato (Solanum lycopersicum) fruit32
Rapid identification of mutations caused by fast neutron bombardment in Medicago truncatula32
An automated method for stem diameter measurement based on laser module and deep learning31
Methods to optimize optical sensing of biotic plant stress – combined effects of hyperspectral imaging at night and spatial binning31
Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken30
Karst vegetation coverage detection using UAV multispectral vegetation indices and machine learning algorithm30
Identifying and mapping individual medicinal plant Lamiophlomis rotata at high elevations by using unmanned aerial vehicles and deep learning28
Improved estimation of aboveground biomass of regional coniferous forests integrating UAV-LiDAR strip data, Sentinel-1 and Sentinel-2 imageries27
Optimized synthesis of layered double hydroxide lactate nanosheets and their biological effects on Arabidopsis seedlings26
AprGPD: the apricot genomic and phenotypic database26
The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma26
Characterizing the effects of different chemicals on stem bending of cut snapdragon flower25
High-throughput phenotyping allows the selection of soybean genotypes for earliness and high grain yield25
A novel visual marker to distinguish haploids from doubled haploids in rice (Oryza sativa, L) at early growth stages24
Improved genomic prediction using machine learning with Variational Bayesian sparsity23
Orchid2024: A cultivar-level dataset and methodology for fine-grained classification of Chinese Cymbidium Orchids23
One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells23
TreeJ: an ImageJ plugin for interactive cell lineage reconstruction from static images23
Combining Fourier-transform infrared spectroscopy and multivariate analysis for chemotyping of cell wall composition in Mungbean (Vigna radiata (L.) Wizcek)23
A systematic high-throughput phenotyping assay for sugarcane stalk quality characterization by near-infrared spectroscopy23
Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology22
Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS22
Advanced screening methods for assessing motility and hatching in plant-parasitic nematodes22
Soybean leaf estimation based on RGB images and machine learning methods22
An efficient screening system of disease-resistant genes from wild apple, Malus sieversii in response to Valsa mali pathogenic fungus22
De novo assembly provides new insights into the evolution of Elaeagnus angustifolia L.21
Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids21
Application of machine learning algorithms and feature selection in rapeseed (Brassica napus L.) breeding for seed yield19
Hyperspectral imaging-based classification of rice leaf blast severity over multiple growth stages19
Efficient isolation and purification of tissue-specific protoplasts from tea plants (Camellia sinensis (L.) O. Kuntze)19
Temporal resolution trumps spectral resolution in UAV-based monitoring of cereal senescence dynamics18
A novel method for extraction of high purity and high production Phytophthora sojae oospores18
Metric learning for image-based flower cultivars identification18
Systematic investigation and validation of peanut genetic transformation via the pollen tube injection method18
Improving the prediction performance of leaf water content by coupling multi-source data with machine learning in rice (Oryza sativa L.)18
Computer vision-based wood identification and its expansion and contribution potentials in wood science: A review17
A deep learning approach to track Arabidopsis seedlings’ circumnutation from time-lapse videos17
A handheld device for measuring the diameter at breast height of individual trees using laser ranging and deep-learning based image recognition17
A label-free, fast and high-specificity technique for plant cell wall imaging and composition analysis17
Non-destructive real-time monitoring of underground root development with distributed fiber optic sensing17
Corn360: a method for quantification of corn kernels17
Segmentation and counting of wheat spike grains based on deep learning and textural feature17
A method to experimentally clamp leaf water content to defined values to assess its effects on apoplastic pH16
Versatile DNA extraction from diverse plant taxa using ionic liquids and magnetic ionic liquids: a methodological breakthrough for enhanced sample utility16
Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study15
Phenotyping seedlings for selection of root system architecture in alfalfa (Medicago sativa L.)15
Estimation of cotton canopy parameters based on unmanned aerial vehicle (UAV) oblique photography15
A CNN model for early detection of pepper Phytophthora blight using multispectral imaging, integrating spectral and textural information15
A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction15
Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE15
In vivo characterisation of field pea stem wall thickness using optical coherence tomography15
Fast estimation of plant growth dynamics using deep neural networks15
Roughness measurement of leaf surface based on shape from focus15
An efficient grafting method for phytoplasma transmission in Catharanthus roseus15
Early detection of verticillium wilt in eggplant leaves by fusing five image channels: a deep learning approach14
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean14
Applying spectral fractal dimension index to predict the SPAD value of rice leaves under bacterial blight disease stress14
HairNet2: deep learning to quantify cotton leaf hairiness, a complex genetic and environmental trait14
Prediction accuracy and repeatability of UAV based biomass estimation in wheat variety trials as affected by variable type, modelling strategy and sampling location14
Ripening dynamics revisited: an automated method to track the development of asynchronous berries on time-lapse images14
A random-sampling approach to track cell divisions in time-lapse fluorescence microscopy14
Study on canopy extraction method for narrowband spectral images based on superpixel color gradation skewness distribution features13
Apnet: Lightweight network for apricot tree disease and pest detection in real-world complex backgrounds13
Classification of multi-year and multi-variety pumpkin seeds using hyperspectral imaging technology and three-dimensional convolutional neural network13
Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts13
Automatic freezing-tolerant rapeseed material recognition using UAV images and deep learning13
A cassava common mosaic virus vector for virus-induced gene silencing in cassava13
Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry13
Can allele-specific loop-mediated isothermal amplification be used for rapid detection of target-site herbicide resistance in Lolium spp.?13
Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants12
Identification of the initial water-site and movement in Gleditsia sinensis seeds and its relation to seed coat structure12
Combining novel feature selection strategy and hyperspectral vegetation indices to predict crop yield12
Tensor methods in data analysis of chromatography/mass spectroscopy-based plant metabolomics12
A streamlined guide RNA screening system for genome editing in Sorghum bicolor12
Correction: Maximizing efficiency in sunflower breeding through historical data optimization11
Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry11
Axial variation in flexural stiffness of plant stem segments: measurement methods and the influence of measurement uncertainty11
Soybean cyst nematode detection and management: a review11
Species-specific basecallers improve actual accuracy of nanopore sequencing in plants11
A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze]11
High-throughput phenotyping for non-destructive estimation of soybean fresh biomass using a machine learning model and temporal UAV data11
A model for genuineness detection in genetically and phenotypically similar maize variety seeds based on hyperspectral imaging and machine learning11
Correction: Revisiting superiority and stability metrics of cultivar performances using genomic data: derivations of new estimators11
A comprehensive review of in planta stable transformation strategies11
Determination of wheat spike and spikelet architecture and grain traits using X-ray Computed Tomography imaging11
Small-scale sequencing enables quality assessment of Ribo-Seq data: an example from Arabidopsis cell culture11
Leaf area index estimation model for UAV image hyperspectral data based on wavelength variable selection and machine learning methods11
Correction: YOLO POD: a fast and accurate multi-task model for dense Soybean Pod counting10
Atmospheric correction of vegetation reflectance with simulation-trained deep learning for ground-based hyperspectral remote sensing10
DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular application10
Terrestrial laser scanning and low magnetic field digitization yield similar architectural coarse root traits for 32-year-old Pinus ponderosa trees10
Optimization of ribosome profiling in plants including structural analysis of rRNA fragments10
Cucumber pathogenic spores’ detection using the GCS-YOLOv8 network with microscopic images in natural scenes10
Applying quantitative spatial phenotypes analysis to the investigation of peltate glandular trichomes development pattern in Perilla frutescens10
Publisher Correction: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i10
Hyperspectral imaging for pest symptom detection in bell pepper10
PhenoTrack3D: an automatic high-throughput phenotyping pipeline to track maize organs over time10
ShinyCore: An R/Shiny program for establishing core collection based on single nucleotide polymorphism data9
Optimization of preparation and transformation of protoplasts from Populus simonii × P. nigra leaves and subcellular localization of the major latex protein 328 (MLP328)9
Cryopreservation of Abies alba × A. numidica and Pinus nigra embryogenic tissues by stepwise dehydration method9
Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture9
Combination of long-term 13CO2 labeling and isotopolog profiling allows turnover analysis of photosynthetic pigments in Arabidopsis leaves9
X-ray driven peanut trait estimation: computer vision aided agri-system transformation9
Gray mold and anthracnose disease detection on strawberry leaves using hyperspectral imaging9
A toolkit to rapidly modify root systems through single plant selection9
Early real-time detection algorithm of tomato diseases and pests in the natural environment9
Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol9
Rice bacterial blight resistant cultivar selection based on visible/near-infrared spectrum and deep learning9
Evaporative flux method of leaf hydraulic conductance estimation: sources of uncertainty and reporting format recommendation9
Exploring genetic variability under extended photoperiod in lentil (Lens Culinaris Medik): vegetative and phenological differentiation according to genetic material’s origins9
Image analysis and polyphenol profiling unveil red-flesh apple phenotype complexity9
Plant diseases and pests detection based on deep learning: a review9
A procedure for Dex-induced gene transactivation in Arabidopsis ovules9
Innovation of strip fertilization planting for rice straw crushing with back-throwing and interrow-laying9
Effects of cushion box and closed let-down ladder usage on damage to corn during handling: physiological deterioration8
Maize-IAS: a maize image analysis software using deep learning for high-throughput plant phenotyping8
Texture feature extraction from microscope images enables a robust estimation of ER body phenotype in Arabidopsis8
A novel in-situ-process technique constructs whole circular cpDNA library8
Feature enhancement guided network for yield estimation of high-density jujube8
K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology8
A technical guide to TRITEX, a computational pipeline for chromosome-scale sequence assembly of plant genomes8
An exploration of the influence of ZnO NPs treatment on germination of radish seeds under salt stress based on the YOLOv8-R lightweight model8
Clarification of the infection pattern of Xanthomonas citri subsp. citri on citrus fruit by artificial inoculation8
Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC)8
A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance8
Meta-analysis of the role of zinc in coordinating absorption of mineral elements in wheat seedlings8
A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum8
Four-dimensional measurement of root system development using time-series three-dimensional volumetric data analysis by backward prediction8
Semantic segmentation of plant roots from RGB (mini-) rhizotron images—generalisation potential and false positives of established methods and advanced deep-learning models8
Improved wood species identification based on multi-view imagery of the three anatomical planes7
‘Single-Seed-SpeedBulks:’ a protocol that combines ‘speed breeding’ with a cost-efficient modified single-seed descent method for rapid-generation-advancement in oat (Avena sativa L.)7
Chemoinformatics-driven classification of Angiosperms using sulfur-containing compounds and machine learning algorithm7
A comprehensive tool in recycling plant-waste of Gossypium barbadense L agricultural and industrial waste extracts containing gossypin and gossypol: hepatoprotective, anti-inflammatory and antioxidant7
Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants7
Deep learning for detecting herbicide weed control spectrum in turfgrass7
Non-wounding contact-based Inoculation of fruits with fungal pathogens in postharvest7
A global non-invasive methodology for the phenotyping of potato under water deficit conditions using imaging, physiological and molecular tools7
Characterization of leaf surface phenotypes based on light interaction7
High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing7
Whole mount multiplexed visualization of DNA, mRNA, and protein in plant-parasitic nematodes7
Computational fluid dynamics simulation analysis of the effect of curved rice leaves on the deposition behaviour of droplets7
Wheat leaf area index prediction using data fusion based on high-resolution unmanned aerial vehicle imagery7
An improved bacterial mRNA enrichment strategy in dual RNA sequencing to unveil the dynamics of plant-bacterial interactions7
Novel in silico screening system for plant defense activators using deep learning-based prediction of reactive oxygen species accumulation7
RhizoMAP: a comprehensive, nondestructive, and sensitive platform for metabolic imaging of the rhizosphere7
MGIDI: a powerful tool to analyze plant multivariate data7
A pin-based probe for electronic moisture meters to determine moisture content in a single wheat kernel7
Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques7
Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis7
Mechanism of self-recovery of hydrophobicity after surface damage of lotus leaf7
Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH7
Comparison of new hyperspectral index and machine learning models for prediction of winter wheat leaf water content7
Correction: Soybean seed pest damage detection method based on spatial frequency domain imaging combined with RL-SVM7
Segmentation and characterization of macerated fibers and vessels using deep learning7
Synchrotron tomography applications in agriculture and food sciences research: a review6
Automated image registration of RGB, hyperspectral and chlorophyll fluorescence imaging data6
A fast method to evaluate in a combinatorial manner the synergistic effect of different biostimulants for promoting growth or tolerance against abiotic stress6
A simple and efficient method to quantify the cell parameters of the seed coat, embryo and silique wall in rapeseed6
Revolutionizing automated pear picking using Mamba architecture6
RiboPlotR: a visualization tool for periodic Ribo-seq reads6
GRABSEEDS: extraction of plant organ traits through image analysis6
Local mapping of root orientation traits by X-ray micro-CT and 3d image analysis: A study case on carrot seedlings grown in simulated vs real weightlessness6
Yield estimation of high-density cotton fields using low-altitude UAV imaging and deep learning6
Targeted mRNA demethylation in Arabidopsis using plant m6A editor6
Optimization of the selection of suitable harvesting periods for medicinal plants: taking Dendrobium officinale as an example6
A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves6
Untapped potential of non-conventional rubus species: bioactivity, nutrition, and livelihood opportunities6
Two Arabidopsis promoters drive seed-coat specific gene expression in pennycress and camelina6
Rapid non-destructive method to phenotype stomatal traits6
An open-source controller to build a dynamic light intensity setup6
Accelerating leaf area measurement using a volumetric approach6
Identifying rice field weeds from unmanned aerial vehicle remote sensing imagery using deep learning6
Crossing multiple life stages: fine-grained classification of agricultural pests6
Computational fluid dynamics simulation experimental verification and analysis of droplets deposition behaviour on vibrating pear leaves6
Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue6
Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement6
New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i5
Field cabbage detection and positioning system based on improved YOLOv8n5
Non-destructive pre-symptomatic detection of gray mold infection in kiwifruit using hyperspectral data and chemometrics5
Phyllotaxis transition over the lifespan of a palm tree using Magnetic Resonance Imaging (MRI) and Terrestrial Laser Scanning (TLS): the case of Jubaea chilensis5
High-throughput phenotyping in maize and soybean genotypes using vegetation indices and computational intelligence5
Raman imaging reveals in-situ microchemistry of cuticle and epidermis of spruce needles5
Development of an infiltration-based RNA preservation method for cryogen-free storage of leaves for gene expression analyses in field-grown plants5
An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster5
Quantitative MRI imaging of parenchyma and venation networks in Brassica napus leaves: effects of development and dehydration5
Overexpressing Arabidopsis thaliana ACBP6 in transgenic rapid-cycling Brassica napus confers cold tolerance5
LysipheN: a gravimetric IoT device for near real-time high-frequency crop phenotyping: a case study on common beans5
Correction: Rapid and cost-effective molecular karyotyping in wheat, barley, and their crossprogeny by chromosome-specific multiplex PCR5
Water and nitrogen in-situ imaging detection in live corn leaves using near-infrared camera and interference filter5
Photolysis of caged cytokinin in single cells of Arabidopsis thaliana5
Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa5
Optimization of a rapid, sensitive, and high throughput molecular sensor to measure canola protoplast respiratory metabolism as a means of screening nanomaterial cytotoxicity5
Propagation of desert moss Syntrichia caninervis in peat pellet: a method for rapidly obtaining large numbers of cloned gametophytes5
Quality assurance of hyperspectral imaging systems for neural network supported plant phenotyping5
Improvement of germination rate and hybridization to facilitate breeding of an industrial oil crop, Euphorbia lagascae Spreng5
Automatic rape flower cluster counting method based on low-cost labelling and UAV-RGB images5
Inter-row navigation line detection for cotton with broken rows5
Correction: A deep learning model for predicting risks of crop pests and diseases from sequential environmental data5
A rapid and non-destructive method for spatial–temporal quantification of colonization by Pseudomonas syringae pv. tomato DC3000 in Arabidopsis and tomato5
Developing non-invasive 3D quantificational imaging for intelligent coconut analysis system with X-ray5
Evaluation of a low-cost staining method for improved visualization of sweet potato whitefly (Bemisia tabaci) eggs on multiple crop plant species5
Fast-track transformation and genome editing in Brachypodium distachyon5
Assembly and operation of an imaging system for long-term monitoring of bioluminescent and fluorescent reporters in plants5
In-vivo Raman microspectroscopy reveals differential nitrate concentration in different developmental zones in Arabidopsis roots5
A new and effective method to induce infection of Phyllachora maydis into corn for tar spot studies in controlled environments5
Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin5
PlantNh-Kcr: a deep learning model for predicting non-histone crotonylation sites in plants5
A high-throughput phenotyping method for sugarcane rind penetrometer resistance and breaking force characterization by near-infrared spectroscopy4
Quantification of spatial metal accumulation patterns in Noccaea caerulescens by X-ray fluorescence image processing for genetic studies4
High-throughput and automatic structural and developmental root phenotyping on Arabidopsis seedlings4
Analysis of canopy phenology in man-made forests using near-earth remote sensing4
Combining the fractional order derivative and machine learning for leaf water content estimation of spring wheat using hyper-spectral indices4
Development of a semi-open chamber system for the gas exchange measurement of whole-canopy under steady and unsteady states in cucumber seedlings4
Automated quantification of fluorescence and morphological changes in pretreated wood cells by fluorescence macroscopy4
Open-source workflow design and management software to interrogate duckweed growth conditions and stress responses4
Establishment of an NPK nutrient monitor system in yield-graded cotton petioles under drip irrigation4
Installation and imaging of thousands of minirhizotrons to phenotype root systems of field-grown plants4
High-resolution spectral information enables phenotyping of leaf epicuticular wax in wheat4
NRTPredictor: identifying rice root cell state in single-cell RNA-seq via ensemble learning4
Comparing DNA isolation methods for forest trees: quality, plastic footprint, and time-efficiency4
WeCoNET: a host–pathogen interactome database for deciphering crucial molecular networks of wheat-common bunt cross-talk mechanisms4
Layering contrasting photoselective filters improves the simulation of foliar shade4
High-throughput color determination of red raspberry puree and correlation of color parameters with total anthocyanins4
The mechanisms and prediction of non-structural carbohydrates accretion and depletion after mechanical wounding in slash pine (Pinus elliottii) using near-infrared reflectance spectroscopy4
MultipleXLab: A high-throughput portable live-imaging root phenotyping platform using deep learning and computer vision4
Fast reconstruction method of three-dimension model based on dual RGB-D cameras for peanut plant4
Using near-infrared spectroscopy to predict nitrogen and phosphorus concentrations of herbarium specimens under different storage conditions4
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