Plant Methods

Article	Citations
Plant diseases and pests detection based on deep learning: a review	349
Early recognition of tomato gray leaf spot disease based on MobileNetv2-YOLOv3 model	121
Semi-supervised few-shot learning approach for plant diseases recognition	74
Leaf area index estimation model for UAV image hyperspectral data based on wavelength variable selection and machine learning methods	69
Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome	69
A survey of few-shot learning in smart agriculture: developments, applications, and challenges	69
High-throughput three-dimensional visualization of root system architecture of rice using X-ray computed tomography	67
Development of support vector machine-based model and comparative analysis with artificial neural network for modeling the plant tissue culture procedures: effect of plant growth regulators on somatic	58
A hybrid model based on general regression neural network and fruit fly optimization algorithm for forecasting and optimizing paclitaxel biosynthesis in Corylus avellana cell culture	53
Wheat ear counting using K-means clustering segmentation and convolutional neural network	44
Accurate machine learning-based germination detection, prediction and quality assessment of three grain crops	44
Remote estimation of leaf area index (LAI) with unmanned aerial vehicle (UAV) imaging for different rice cultivars throughout the entire growing season	43
Yield prediction by machine learning from UAS-based multi-sensor data fusion in soybean	43
High throughput analysis of leaf chlorophyll content in sorghum using RGB, hyperspectral, and fluorescence imaging and sensor fusion	42
Machine learning for high-throughput field phenotyping and image processing provides insight into the association of above and below-ground traits in cassava (Manihot esculenta Crantz)	42
Few-shot cotton leaf spots disease classification based on metric learning	40
Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays	38
Early real-time detection algorithm of tomato diseases and pests in the natural environment	37
Computer vision-based wood identification and its expansion and contribution potentials in wood science: A review	37
Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer	36
Phenotypic techniques and applications in fruit trees: a review	36
Deep learning-based detection of seedling development	35
Evaluation of novel precision viticulture tool for canopy biomass estimation and missing plant detection based on 2.5D and 3D approaches using RGB images acquired by UAV platform	35
Heritable gene editing using FT mobile guide RNAs and DNA viruses	35
Recent developments and emerging trends of mass spectrometric methods in plant hormone analysis: a review	35

DeepFlower: a deep learning-based approach to characterize flowering patterns of cotton plants in the field	35
Isolation of antimicrobial peptides from different plant sources: Does a general extraction method exist?	34
Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry	33
Method for accurate multi-growth-stage estimation of fractional vegetation cover using unmanned aerial vehicle remote sensing	33
Non-destructive estimation of field maize biomass using terrestrial lidar: an evaluation from plot level to individual leaf level	32
Estimation of plant height and yield based on UAV imagery in faba bean (Vicia faba L.)	31
An improved strategy to analyse strigolactones in complex sample matrices using UHPLC–MS/MS	31
Identification of stable reference genes for qPCR studies in common wheat (Triticum aestivum L.) seedlings under short-term drought stress	31
SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species	31
Raman imaging reveals in-situ microchemistry of cuticle and epidermis of spruce needles	30
A robust spectral angle index for remotely assessing soybean canopy chlorophyll content in different growing stages	30
CRISPR-Cas9 enrichment and long read sequencing for fine mapping in plants	30
Optimization of protoplast regeneration in the model plant Arabidopsis thaliana	30
Efficient chromatin profiling of H3K4me3 modification in cotton using CUT&Tag	30
Maize tassels detection: a benchmark of the state of the art	30
Analysis of trace metal distribution in plants with lab-based microscopic X-ray fluorescence imaging	29
A detached leaf assay for testing transient gene expression and gene editing in cowpea (Vigna unguiculata [L.] Walp.)	29
Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants	29
DiCoExpress: a tool to process multifactorial RNAseq experiments from quality controls to co-expression analysis through differential analysis based on contrasts inside GLM models	28
High-throughput soybean seeds phenotyping with convolutional neural networks and transfer learning	28
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean	28
Wheat physiology predictor: predicting physiological traits in wheat from hyperspectral reflectance measurements using deep learning	28
An in situ approach to characterizing photosynthetic gas exchange of rice panicle	27
A hybrid CNN–SVM classifier for weed recognition in winter rape field	26
Segmentation of structural parts of rosebush plants with 3D point-based deep learning methods	26
Raman spectroscopy enables phenotyping and assessment of nutrition values of plants: a review	25
Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton	25
Nutrient solutions for Arabidopsis thaliana: a study on nutrient solution composition in hydroponics systems	25
Prediction of hypericin content in Hypericum perforatum L. in different ecological habitat using artificial neural networks	25
Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization	24
DNA–protein interaction studies: a historical and comparative analysis	24
Nondestructive estimation of potato yield using relative variables derived from multi-period LAI and hyperspectral data based on weighted growth stage	24
An efficient in vitro regeneration system from different wild apple (Malus sieversii) explants	24
Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing	24
The effect of plant weight on estimations of stalk lodging resistance	24
Multispectral and X-ray images for characterization of Jatropha curcas L. seed quality	23
Optimization of isolation and transfection conditions of maize endosperm protoplasts	23
Leaf infiltration in plant science: old method, new possibilities	23
A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants	23
Chlorophyll fluorescence imaging captures photochemical efficiency of grain sorghum (Sorghum bicolor) in a field setting	23
Genomic prediction of yield and root development in wheat under changing water availability	23
An improved method for the segmentation of roots from X-ray computed tomography 3D images: Rootine v.2	22
Determination of wheat spike and spikelet architecture and grain traits using X-ray Computed Tomography imaging	22
Evaluation of maize lodging resistance based on the critical wind speed of stalk breaking during the late growth stage	22
A cassava common mosaic virus vector for virus-induced gene silencing in cassava	22
Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato	21
The BELT and phenoSEED platforms: shape and colour phenotyping of seed samples	21
Extraction of tree crowns damaged by Dendrolimus tabulaeformis Tsai et Liu via spectral-spatial classification using UAV-based hyperspectral images	21
A CRISPR/dCas9 toolkit for functional analysis of maize genes	21
An efficient system for Agrobacterium-mediated transient transformation in Pinus tabuliformis	20

Deep learning for detecting herbicide weed control spectrum in turfgrass	20
A glass bead semi-hydroponic system for intact maize root exudate analysis and phenotyping	20
Comparison of new hyperspectral index and machine learning models for prediction of winter wheat leaf water content	20
Leaf to panicle ratio (LPR): a new physiological trait indicative of source and sink relation in japonica rice based on deep learning	20
Agrobacterium rhizogenes-mediated hairy root transformation as an efficient system for gene function analysis in Litchi chinensis	20
Diverse maize hybrids are structurally inefficient at resisting wind induced bending forces that cause stalk lodging	20
Predictive modeling of Persian walnut (Juglans regia L.) in vitro proliferation media using machine learning approaches: a comparative study of ANN, KNN and GEP models	19
Wheat leaf area index prediction using data fusion based on high-resolution unmanned aerial vehicle imagery	19
Prediction of plant-level tomato biomass and yield using machine learning with unmanned aerial vehicle imagery	19
Maize-IAS: a maize image analysis software using deep learning for high-throughput plant phenotyping	19
Gene Ontology Meta Annotator for Plants (GOMAP)	19
A rapid and sensitive method to assess seed longevity through accelerated aging in an invasive plant species	18
A model for genuineness detection in genetically and phenotypically similar maize variety seeds based on hyperspectral imaging and machine learning	18
A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.	17
Non-destructive identification of single hard seed via multispectral imaging analysis in six legume species	17
Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.)	17
A generalised approach for high-throughput instance segmentation of stomata in microscope images	17
Analytical methods in strigolactone research	17
Predicting yellow rust in wheat breeding trials by proximal phenotyping and machine learning	16
Electrical impedance spectroscopy (EIS) in plant roots research: a review	16
L2MXception: an improved Xception network for classification of peach diseases	16
Evaluating the suitability of hyper- and multispectral imaging to detect foliar symptoms of the grapevine trunk disease Esca in vineyards	16
Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation of Agapanthus praecox embryogenic callus	15
A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues	15
A novel rind puncture technique to measure rind thickness and diameter in plant stalks	15
Improving the estimation of alpine grassland fractional vegetation cover using optimized algorithms and multi-dimensional features	15
A simple and fast Agrobacterium-mediated transformation system for passion fruit KPF4 (Passiflora edulis f. edulis × Passiflora edulis f. flavicarpa)	15
Meta-learning prediction of physical and chemical properties of magnetized water and fertilizer based on LSTM	15
Rice bacterial blight resistant cultivar selection based on visible/near-infrared spectrum and deep learning	15
Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)	15
Anthocyanin, a novel and user-friendly reporter for convenient, non-destructive, low cost, directly visual selection of transgenic hairy roots in the study of rhizobia-legume symbiosis	15
Automated discretization of ‘transpiration restriction to increasing VPD’ features from outdoors high-throughput phenotyping data	15
High-throughput method for detection and quantification of lesions on leaf scale based on trypan blue staining and digital image analysis	15
Efficient isolation and purification of tissue-specific protoplasts from tea plants (Camellia sinensis (L.) O. Kuntze)	14
Sensor-based phenotyping of above-ground plant-pathogen interactions	14
A modified method for enzymatic isolation of and subsequent wax extraction from Arabidopsis thaliana leaf cuticle	14
Attention-optimized DeepLab V3 + for automatic estimation of cucumber disease severity	14
Microscale thermophoresis as a powerful tool for screening glycosyltransferases involved in cell wall biosynthesis	14
Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays	14
TopoRoot: a method for computing hierarchy and fine-grained traits of maize roots from 3D imaging	14
X-ray fluorescence spectroscopy (XRF) for metallome analysis of herbarium specimens	13
Development of a universal endogenous qPCR control for eukaryotic DNA samples	13
Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology	13
A method for experimental warming of developing tree seeds with a common garden demonstration of seedling responses	13
An automatic method for counting wheat tiller number in the field with terrestrial LiDAR	13
Sweet cherry fruit cracking: follow-up testing methods and cultivar-metabolic screening	13
Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion bioreactor system and MeJA elicitation	13
Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts	13
Development of an accurate low cost NDVI imaging system for assessing plant health	12
Interest of phenomic prediction as an alternative to genomic prediction in grapevine	12
YOLO POD: a fast and accurate multi-task model for dense Soybean Pod counting	12
MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactions	12
Integrated Puncture Score: force–displacement weighted rind penetration tests improve stalk lodging resistance estimations in maize	12
Soybean cyst nematode detection and management: a review	12
Agrobacterium tumefaciens mediated transformation of the aquatic carnivorous plant Utricularia gibba	12
Deep learning based high-throughput phenotyping of chalkiness in rice exposed to high night temperature	12
Sorting biotic and abiotic stresses on wild rocket by leaf-image hyperspectral data mining with an artificial intelligence model	12
Biomass estimation of cultivated red algae Pyropia using unmanned aerial platform based multispectral imaging	12
The platform GrowScreen-Agar enables identification of phenotypic diversity in root and shoot growth traits of agar grown plants	12
Combining novel feature selection strategy and hyperspectral vegetation indices to predict crop yield	12
A practical guide to estimating the light extinction coefficient with nonlinear models—a case study on maize	12
Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level	12
Non-destructive phenotyping for early seedling vigor in direct-seeded rice	12
Experimental comparison of two methods to study barley responses to partial submergence	12
Reconstruction method and optimum range of camera-shooting angle for 3D plant modeling using a multi-camera photography system	12
Preparation of uniformly labelled 13C- and 15N-plants using customised growth chambers	12
Estimation of cotton canopy parameters based on unmanned aerial vehicle (UAV) oblique photography	12
High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing	11
An optimized histological proceeding to study the female gametophyte development in grapevine	11
Core-labelling technique (CLT): a novel combination of the ingrowth-core method and tracer technique for deep root study	11
Ice accommodation in plant tissues pinpointed by cryo-microscopy in reflected-polarised-light	11
Continuous monitoring of plant sodium transport dynamics using clinical PET	11
MultipleXLab: A high-throughput portable live-imaging root phenotyping platform using deep learning and computer vision	11
Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue	11
New methods for confocal imaging of infection threads in crop and model legumes	11
4D Structural root architecture modeling from digital twins by X-Ray Computed Tomography	11
Automated, image-based disease measurement for phenotyping resistance to soybean frogeye leaf spot	11
A systematic high-throughput phenotyping assay for sugarcane stalk quality characterization by near-infrared spectroscopy	11
DiSCount: computer vision for automated quantification of Striga seed germination	10
A comparison of ImageJ and machine learning based image analysis methods to measure cassava bacterial blight disease severity	10

A fast method to evaluate in a combinatorial manner the synergistic effect of different biostimulants for promoting growth or tolerance against abiotic stress	10
High-throughput phenotyping allows the selection of soybean genotypes for earliness and high grain yield	10
All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing	10
New field wind manipulation methodology reveals adaptive responses of steppe plants to increased and reduced wind speed	10
Development of a fuzzy model for differentiating peanut plant from broadleaf weeds using image features	10
3D characterization of walnut morphological traits using X-ray computed tomography	10
Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids	10
A handheld device for measuring the diameter at breast height of individual trees using laser ranging and deep-learning based image recognition	10
Leaf reflectance can surrogate foliar economics better than physiological traits across macrophyte species	10
High throughput phenotyping of cross-sectional morphology to assess stalk lodging resistance	10
Rapid non-destructive method to phenotype stomatal traits	10
Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors	10
A toolkit to rapidly modify root systems through single plant selection	9
Automatic freezing-tolerant rapeseed material recognition using UAV images and deep learning	9
Phylogenomic synteny network analyses reveal ancestral transpositions of auxin response factor genes in plants	9
HairNet: a deep learning model to score leaf hairiness, a key phenotype for cotton fibre yield, value and insect resistance	9
Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants	9
Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE	9
Methodology: an optimized, high-yield tomato leaf chloroplast isolation and stroma extraction protocol for proteomics analyses and identification of chloroplast co-localizing proteins	9
Quantitative phenotyping and evaluation for lettuce leaves of multiple semantic components	9
On the applicability of the Tubulin-Based Polymorphism (TBP) genotyping method: a comprehensive guide illustrated through the application on different genetic resources in the legume family	9
Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis	9
A label-free, fast and high-specificity technique for plant cell wall imaging and composition analysis	9
An efficient overexpression method for studying genes in Ricinus that transport vectorized agrochemicals	9
Automatic kernel counting on maize ear using RGB images	9
Rapid metabolite response in leaf blade and petiole as a marker for shade avoidance syndrome	9
Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa	8
High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing	8
Roughness measurement of leaf surface based on shape from focus	8
High-efficiency procedure to characterize, segment, and quantify complex multicellularity in raw micrographs in plants	8
Fast reconstruction method of three-dimension model based on dual RGB-D cameras for peanut plant	8
An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster	8
Arabidopsis guard cell CO2/HCO3− response mutant screening by an aequorin-based calcium imaging system	8
A simple, cost-effective high-throughput image analysis pipeline improves genomic prediction accuracy for days to maturity in wheat	8
Inter-row navigation line detection for cotton with broken rows	8
Yield estimation of high-density cotton fields using low-altitude UAV imaging and deep learning	8
THEMS: an automated thermal and hyperspectral proximal sensing system for canopy reflectance, radiance and temperature	8
Metric learning for image-based flower cultivars identification	8
A modified aeroponic system for growing small-seeded legumes and other plants to study root systems	8
DeepCob: precise and high-throughput analysis of maize cob geometry using deep learning with an application in genebank phenomics	7
Field-measured canopy height may not be as accurate and heritable as believed: evidence from advanced 3D sensing	7
A low-cost automated growth chamber system for continuous measurements of gas exchange at canopy scale in dynamic conditions	7
Cross-sectional geometry predicts failure location in maize stalks	7
Using Near-infrared reflectance spectroscopy (NIRS) to predict glucobrassicin concentrations in cabbage and brussels sprout leaf tissue	7
Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis	7
Computational fluid dynamics simulation experimental verification and analysis of droplets deposition behaviour on vibrating pear leaves	7
Methods of analysis of chloroplast genomes of C3, Kranz type C4 and Single Cell C4 photosynthetic members of Chenopodiaceae	7
Improving the sorting efficiency of maize haploid kernels using an NMR-based method with oil content double thresholds	7
Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries	7
Floral infrared emissivity estimates using simple tools	7
Peat-based hairy root transformation using Rhizobium rhizogenes as a rapid and efficient tool for easily exploring potential genes related to root-knot nematode parasitism and host response	7
Complete root specimen of plants grown in soil-filled root box: sampling, measuring, and staining method	7
Water and nitrogen in-situ imaging detection in live corn leaves using near-infrared camera and interference filter	7
Automatic rape flower cluster counting method based on low-cost labelling and UAV-RGB images	7
A global non-invasive methodology for the phenotyping of potato under water deficit conditions using imaging, physiological and molecular tools	7
A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum	7
Applying spectral fractal dimension index to predict the SPAD value of rice leaves under bacterial blight disease stress	7
Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)	7
Open source 3D phenotyping of chickpea plant architecture across plant development	7
Efficient strategies for controlled release of nanoencapsulated phytohormones to improve plant stress tolerance	7
Cowpea speed breeding using regulated growth chamber conditions and seeds of oven-dried immature pods potentially accommodates eight generations per year	7
Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa	7
Big data from small tissues: extraction of high-quality RNA for RNA-sequencing from different oilseed Brassica seed tissues during seed development	7
The effect of probe geometry on rind puncture resistance testing of maize stalks	7
Synchrotron tomography applications in agriculture and food sciences research: a review	7
Species-specific basecallers improve actual accuracy of nanopore sequencing in plants	6
A heat-shock inducible system for flexible gene expression in cereals	6
Image analysis for the automatic phenotyping of Orobanche cumana tubercles on sunflower roots	6
An improved pollen number counting method using a cell counter and mesh columns	6
Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter	6
High throughput profiling of tocochromanols in leaves and seeds of Arabidopsis and Maize	6
A method of two-dimensional correlation spectroscopy combined with residual neural network for comparison and differentiation of medicinal plants raw materials superior to traditional machine learning	6
Installation and imaging of thousands of minirhizotrons to phenotype root systems of field-grown plants	6
Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques	6
A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in Arabidopsis	6
Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge	6
Wheat grain width: a clue for re-exploring visual indicators of grain weight	6
Real-time monitoring of rhizosphere nitrate fluctuations under crops following defoliation	6
Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method	6
Cotton stubble detection based on wavelet decomposition and texture features	6
A cell suspension based uptake method to study high affinity glucosinolate transporters	6
Simultaneous silencing of two different Arabidopsis genes with a novel virus-induced gene silencing vector	6
A method for obtaining field wheat freezing injury phenotype based on RGB camera and software control	6
Texture feature extraction from microscope images enables a robust estimation of ER body phenotype in Arabidopsis	6
Rapid, high efficiency virus-mediated mutant complementation and gene silencing in Antirrhinum	6
Phenotyping seedlings for selection of root system architecture in alfalfa (Medicago sativa L.)	6
HANDY: a device for assessing resistance to mechanical crushing of maize kernel	6
A two-step registration-classification approach to automated segmentation of multimodal images for high-throughput greenhouse plant phenotyping	6
Fast and easy bioassay for the necrotizing fungus Botrytis cinerea on poplar leaves	6
Depth image conversion model based on CycleGAN for growing tomato truss identification	6
Banana plant counting and morphological parameters measurement based on terrestrial laser scanning	6
WeCoNET: a host–pathogen interactome database for deciphering crucial molecular networks of wheat-common bunt cross-talk mechanisms	6
Quantification of spatial metal accumulation patterns in Noccaea caerulescens by X-ray fluorescence image processing for genetic studies	6
Characterizing the effects of different chemicals on stem bending of cut snapdragon flower	6
A new technical approach for preparing frozen biological samples for electron microscopy	5