Plant Methods

Article	Citations
Plant diseases and pests detection based on deep learning: a review	483
Leaf area index estimation model for UAV image hyperspectral data based on wavelength variable selection and machine learning methods	85
Semi-supervised few-shot learning approach for plant diseases recognition	79
A survey of few-shot learning in smart agriculture: developments, applications, and challenges	77
A hybrid model based on general regression neural network and fruit fly optimization algorithm for forecasting and optimizing paclitaxel biosynthesis in Corylus avellana cell culture	59
Remote estimation of leaf area index (LAI) with unmanned aerial vehicle (UAV) imaging for different rice cultivars throughout the entire growing season	57
High throughput analysis of leaf chlorophyll content in sorghum using RGB, hyperspectral, and fluorescence imaging and sensor fusion	54
Screening natural product extracts for potential enzyme inhibitors: protocols, and the standardisation of the usage of blanks in α-amylase, α-glucosidase and lipase assays	53
Accurate machine learning-based germination detection, prediction and quality assessment of three grain crops	52
Estimation of plant height and yield based on UAV imagery in faba bean (Vicia faba L.)	51
Few-shot cotton leaf spots disease classification based on metric learning	50
Early real-time detection algorithm of tomato diseases and pests in the natural environment	47
Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry	45
Computer vision-based wood identification and its expansion and contribution potentials in wood science: A review	43
Quantitative visualization of photosynthetic pigments in tea leaves based on Raman spectroscopy and calibration model transfer	42
DeepFlower: a deep learning-based approach to characterize flowering patterns of cotton plants in the field	38
Heritable gene editing using FT mobile guide RNAs and DNA viruses	37
Method for accurate multi-growth-stage estimation of fractional vegetation cover using unmanned aerial vehicle remote sensing	36
Leaf infiltration in plant science: old method, new possibilities	36
Optimization of protoplast regeneration in the model plant Arabidopsis thaliana	36
High-throughput soybean seeds phenotyping with convolutional neural networks and transfer learning	35
Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants	35
A hybrid CNN–SVM classifier for weed recognition in winter rape field	35
Raman imaging reveals in-situ microchemistry of cuticle and epidermis of spruce needles	34
Wheat physiology predictor: predicting physiological traits in wheat from hyperspectral reflectance measurements using deep learning	34

Soybean cyst nematode detection and management: a review	33
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean	32
DNA–protein interaction studies: a historical and comparative analysis	32
Raman spectroscopy enables phenotyping and assessment of nutrition values of plants: a review	31
Segmentation of structural parts of rosebush plants with 3D point-based deep learning methods	31
Nondestructive estimation of potato yield using relative variables derived from multi-period LAI and hyperspectral data based on weighted growth stage	31
Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton	30
A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants	30
Development of an accurate low cost NDVI imaging system for assessing plant health	30
Prediction of hypericin content in Hypericum perforatum L. in different ecological habitat using artificial neural networks	28
Determination of wheat spike and spikelet architecture and grain traits using X-ray Computed Tomography imaging	28
A glass bead semi-hydroponic system for intact maize root exudate analysis and phenotyping	26
Agrobacterium rhizogenes-mediated hairy root transformation as an efficient system for gene function analysis in Litchi chinensis	26
A cassava common mosaic virus vector for virus-induced gene silencing in cassava	26
Improving the estimation of alpine grassland fractional vegetation cover using optimized algorithms and multi-dimensional features	25
Prediction of plant-level tomato biomass and yield using machine learning with unmanned aerial vehicle imagery	25
Multispectral and X-ray images for characterization of Jatropha curcas L. seed quality	25
Evaluation of maize lodging resistance based on the critical wind speed of stalk breaking during the late growth stage	25
Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing	25
Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato	24
Gene Ontology Meta Annotator for Plants (GOMAP)	24
An improved method for the segmentation of roots from X-ray computed tomography 3D images: Rootine v.2	23
Maize-IAS: a maize image analysis software using deep learning for high-throughput plant phenotyping	23
Comparison of new hyperspectral index and machine learning models for prediction of winter wheat leaf water content	23
A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.	23
Wheat leaf area index prediction using data fusion based on high-resolution unmanned aerial vehicle imagery	23
Predicting yellow rust in wheat breeding trials by proximal phenotyping and machine learning	22
A model for genuineness detection in genetically and phenotypically similar maize variety seeds based on hyperspectral imaging and machine learning	22
Attention-optimized DeepLab V3 + for automatic estimation of cucumber disease severity	22
Electrical impedance spectroscopy (EIS) in plant roots research: a review	22
Sensor-based phenotyping of above-ground plant-pathogen interactions	22
Predictive modeling of Persian walnut (Juglans regia L.) in vitro proliferation media using machine learning approaches: a comparative study of ANN, KNN and GEP models	22
Rice bacterial blight resistant cultivar selection based on visible/near-infrared spectrum and deep learning	21
Deep learning for detecting herbicide weed control spectrum in turfgrass	21
A generalised approach for high-throughput instance segmentation of stomata in microscope images	19
TopoRoot: a method for computing hierarchy and fine-grained traits of maize roots from 3D imaging	19
L2MXception: an improved Xception network for classification of peach diseases	18
Complementarity of Raman and Infrared spectroscopy for rapid characterization of fucoidan extracts	18
High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing	18
Sorting biotic and abiotic stresses on wild rocket by leaf-image hyperspectral data mining with an artificial intelligence model	18
Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays	18
Rapid non-destructive method to phenotype stomatal traits	18
Biomass estimation of cultivated red algae Pyropia using unmanned aerial platform based multispectral imaging	17
Combining novel feature selection strategy and hyperspectral vegetation indices to predict crop yield	17
X-ray fluorescence spectroscopy (XRF) for metallome analysis of herbarium specimens	17
YOLO POD: a fast and accurate multi-task model for dense Soybean Pod counting	17
Meta-learning prediction of physical and chemical properties of magnetized water and fertilizer based on LSTM	17
Deep learning based high-throughput phenotyping of chalkiness in rice exposed to high night temperature	17
A comprehensive comparison of four methods for extracting lipids from Arabidopsis tissues	17
MGIDI: a powerful tool to analyze plant multivariate data	17

Interest of phenomic prediction as an alternative to genomic prediction in grapevine	17
New field wind manipulation methodology reveals adaptive responses of steppe plants to increased and reduced wind speed	16
MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactions	16
Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level	16
Species-specific basecallers improve actual accuracy of nanopore sequencing in plants	16
A method for experimental warming of developing tree seeds with a common garden demonstration of seedling responses	15
Fast reconstruction method of three-dimension model based on dual RGB-D cameras for peanut plant	15
Standardization of electrolyte leakage data and a novel liquid nitrogen control improve measurements of cold hardiness in woody tissue	15
4D Structural root architecture modeling from digital twins by X-Ray Computed Tomography	15
WheatLFANet: in-field detection and counting of wheat heads with high-real-time global regression network	15
Yield estimation of high-density cotton fields using low-altitude UAV imaging and deep learning	15
Efficient isolation and purification of tissue-specific protoplasts from tea plants (Camellia sinensis (L.) O. Kuntze)	15
How to make sense of 3D representations for plant phenotyping: a compendium of processing and analysis techniques	15
High throughput phenotyping of cross-sectional morphology to assess stalk lodging resistance	15
A fast method to evaluate in a combinatorial manner the synergistic effect of different biostimulants for promoting growth or tolerance against abiotic stress	15
A modified aeroponic system for growing small-seeded legumes and other plants to study root systems	14
A comparison of ImageJ and machine learning based image analysis methods to measure cassava bacterial blight disease severity	14
High-throughput phenotyping allows the selection of soybean genotypes for earliness and high grain yield	14
MultipleXLab: A high-throughput portable live-imaging root phenotyping platform using deep learning and computer vision	14
A handheld device for measuring the diameter at breast height of individual trees using laser ranging and deep-learning based image recognition	14
Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion bioreactor system and MeJA elicitation	14
Automated, image-based disease measurement for phenotyping resistance to soybean frogeye leaf spot	14
Real-time monitoring of Ralstonia solanacearum infection progress in tomato and Arabidopsis using bioluminescence imaging technology	14
A deep learning model for rapid classification of tea coal disease	13
Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants	13
Estimation of cotton canopy parameters based on unmanned aerial vehicle (UAV) oblique photography	13
Experimental comparison of two methods to study barley responses to partial submergence	13
Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis	13
A practical guide to estimating the light extinction coefficient with nonlinear models—a case study on maize	13
Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 genome-editing system to Brassica rapa var. rapa	13
A systematic high-throughput phenotyping assay for sugarcane stalk quality characterization by near-infrared spectroscopy	13
New methods for confocal imaging of infection threads in crop and model legumes	12
Synchrotron tomography applications in agriculture and food sciences research: a review	12
Efficient strategies for controlled release of nanoencapsulated phytohormones to improve plant stress tolerance	12
Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids	12
Continuous monitoring of plant sodium transport dynamics using clinical PET	12
Computational fluid dynamics simulation experimental verification and analysis of droplets deposition behaviour on vibrating pear leaves	12
Leaf reflectance can surrogate foliar economics better than physiological traits across macrophyte species	11
Development of a fuzzy model for differentiating peanut plant from broadleaf weeds using image features	11
Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques	11
Phenotyping seedlings for selection of root system architecture in alfalfa (Medicago sativa L.)	11
Inter-row navigation line detection for cotton with broken rows	11
Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa	11
Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE	11
A label-free, fast and high-specificity technique for plant cell wall imaging and composition analysis	11
Cowpea speed breeding using regulated growth chamber conditions and seeds of oven-dried immature pods potentially accommodates eight generations per year	11
A method of two-dimensional correlation spectroscopy combined with residual neural network for comparison and differentiation of medicinal plants raw materials superior to traditional machine learning	11
A toolkit to rapidly modify root systems through single plant selection	11
Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors	11
Automatic rape flower cluster counting method based on low-cost labelling and UAV-RGB images	10
Roughness measurement of leaf surface based on shape from focus	10
An efficient overexpression method for studying genes in Ricinus that transport vectorized agrochemicals	10
Quantitative phenotyping and evaluation for lettuce leaves of multiple semantic components	10
HairNet: a deep learning model to score leaf hairiness, a key phenotype for cotton fibre yield, value and insect resistance	10
Applying spectral fractal dimension index to predict the SPAD value of rice leaves under bacterial blight disease stress	10
Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries	10
Banana plant counting and morphological parameters measurement based on terrestrial laser scanning	10
Improving the sorting efficiency of maize haploid kernels using an NMR-based method with oil content double thresholds	10
Mapping the forage nitrogen, phosphorus, and potassium contents of alpine grasslands by integrating Sentinel-2 and Tiangong-2 data	10
Improved weed segmentation in UAV imagery of sorghum fields with a combined deblurring segmentation model	10
An optimized pipeline for live imaging whole Arabidopsis leaves at cellular resolution	9
Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter	9
3D-GBS: a universal genotyping-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes	9
A global non-invasive methodology for the phenotyping of potato under water deficit conditions using imaging, physiological and molecular tools	9
Automatic freezing-tolerant rapeseed material recognition using UAV images and deep learning	9
Identifying and mapping individual medicinal plant Lamiophlomis rotata at high elevations by using unmanned aerial vehicles and deep learning	9
Field-measured canopy height may not be as accurate and heritable as believed: evidence from advanced 3D sensing	9
Peat-based hairy root transformation using Rhizobium rhizogenes as a rapid and efficient tool for easily exploring potential genes related to root-knot nematode parasitism and host response	9
Effect of a suitable treatment period on the genetic transformation efficiency of the plant leaf disc method	9
The impact of variable illumination on vegetation indices and evaluation of illumination correction methods on chlorophyll content estimation using UAV imagery	9
High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing	9
Metric learning for image-based flower cultivars identification	9
A simple, cost-effective high-throughput image analysis pipeline improves genomic prediction accuracy for days to maturity in wheat	9
DeepCob: precise and high-throughput analysis of maize cob geometry using deep learning with an application in genebank phenomics	9
Floral infrared emissivity estimates using simple tools	9
Cross-sectional geometry predicts failure location in maize stalks	9
An efficient CRISPR-Cas9 enrichment sequencing strategy for characterizing complex and highly duplicated genomic regions. A case study in the Prunus salicina LG3-MYB10 genes cluster	9
Segmentation and counting of wheat spike grains based on deep learning and textural feature	9
Fast estimation of plant growth dynamics using deep neural networks	9
Simultaneous silencing of two different Arabidopsis genes with a novel virus-induced gene silencing vector	8

Remotely sensed estimation of total iron content in soil with harmonic analysis and BP neural network	8
Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis	8
Image analysis for the automatic phenotyping of Orobanche cumana tubercles on sunflower roots	8
Super-resolution imaging of Douglas fir xylem cell wall nanostructure using SRRF microscopy	8
Fast and easy bioassay for the necrotizing fungus Botrytis cinerea on poplar leaves	8
A method for obtaining field wheat freezing injury phenotype based on RGB camera and software control	8
Propagation of desert moss Syntrichia caninervis in peat pellet: a method for rapidly obtaining large numbers of cloned gametophytes	8
Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera	8
A low-cost automated growth chamber system for continuous measurements of gas exchange at canopy scale in dynamic conditions	8
A hyperspectral deep learning attention model for predicting lettuce chlorophyll content	8
Water and nitrogen in-situ imaging detection in live corn leaves using near-infrared camera and interference filter	8
Installation and imaging of thousands of minirhizotrons to phenotype root systems of field-grown plants	8
Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)	8
Carbon dioxide expanded liquid: an effective solvent for the extraction of quercetin from South African medicinal plants	8
Complete root specimen of plants grown in soil-filled root box: sampling, measuring, and staining method	8
Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis	7
Texture feature extraction from microscope images enables a robust estimation of ER body phenotype in Arabidopsis	7
Real-time monitoring of rhizosphere nitrate fluctuations under crops following defoliation	7
Characterizing the effects of different chemicals on stem bending of cut snapdragon flower	7
Pseudo high-frequency boosts the generalization of a convolutional neural network for cassava disease detection	7
Rapid monitoring of flavonoid content in sweet tea (Lithocarpus litseifolius (Hance) Chun) leaves using NIR spectroscopy	7
Improving multi-scale detection layers in the deep learning network for wheat spike detection based on interpretive analysis	7
Quantification of the three-dimensional root system architecture using an automated rotating imaging system	7
Untapped potential of non-conventional rubus species: bioactivity, nutrition, and livelihood opportunities	7
Wheat grain width: a clue for re-exploring visual indicators of grain weight	7
Classification of multi-year and multi-variety pumpkin seeds using hyperspectral imaging technology and three-dimensional convolutional neural network	7
Plant trait measurement in 3D for growth monitoring	7
Identification of the initial water-site and movement in Gleditsia sinensis seeds and its relation to seed coat structure	7
Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology	7
Laser-based molecular delivery and its applications in plant science	7
WeCoNET: a host–pathogen interactome database for deciphering crucial molecular networks of wheat-common bunt cross-talk mechanisms	7
An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome	7
A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves	7
A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum	7
TSWIFT: Tower Spectrometer on Wheels for Investigating Frequent Timeseries for high-throughput phenotyping of vegetation physiology	7
Hyperspectral imaging-based classification of rice leaf blast severity over multiple growth stages	7
Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples	7
Open source 3D phenotyping of chickpea plant architecture across plant development	7
A deep learning model for predicting risks of crop pests and diseases from sequential environmental data	7
A rapid and non-destructive method for spatial–temporal quantification of colonization by Pseudomonas syringae pv. tomato DC3000 in Arabidopsis and tomato	7
Depth image conversion model based on CycleGAN for growing tomato truss identification	6
High-resolution spectral information enables phenotyping of leaf epicuticular wax in wheat	6
Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells	6
Characterization of leaf surface phenotypes based on light interaction	6
Application of machine learning algorithms and feature selection in rapeseed (Brassica napus L.) breeding for seed yield	6
A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in Arabidopsis	6
Experimental data manipulations to assess performance of hyperspectral classification models of crop seeds and other objects	6
HANDY: a device for assessing resistance to mechanical crushing of maize kernel	6
Cotton stubble detection based on wavelet decomposition and texture features	6
A low-cost and open-source solution to automate imaging and analysis of cyst nematode infection assays for Arabidopsis thaliana	6
A foliar pigment-based bioassay for interrogating chloroplast signalling revealed that carotenoid isomerisation regulates chlorophyll abundance	6
Cotton plant part 3D segmentation and architectural trait extraction using point voxel convolutional neural networks	6
Targeted mRNA demethylation in Arabidopsis using plant m6A editor	6
Optimized synthesis of layered double hydroxide lactate nanosheets and their biological effects on Arabidopsis seedlings	6
A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol	6
Optimization of immune receptor-related hypersensitive cell death response assay using agrobacterium-mediated transient expression in tobacco plants	6
DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate	6
Application of X-ray computed tomography to analyze the structure of sorghum grain	6
Evaluating potential of leaf reflectance spectra to monitor plant genetic variation	6
Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement	6
Atmospheric correction of vegetation reflectance with simulation-trained deep learning for ground-based hyperspectral remote sensing	6
Full-length transcriptome sequences by a combination of sequencing platforms applied to isoflavonoid and triterpenoid saponin biosynthesis of Astragalus mongholicus Bunge	6
NADPH containing superoxide-producing thermostable complex from raspberry, apricot, grape, and grape seeds: isolation, purification, and properties	6
Genotyping of polyploid plants using quantitative PCR: application in the breeding of white-fleshed triploid loquats (Eriobotrya japonica)	6
SeptoSympto: a precise image analysis of Septoria tritici blotch disease symptoms using deep learning methods on scanned images	5
Evaluation of an intelligent artificial climate chamber for high-throughput crop phenotyping in wheat	5
A protocol for Chenopodium quinoa pollen germination	5
Non-destructive pre-symptomatic detection of gray mold infection in kiwifruit using hyperspectral data and chemometrics	5
PhenoTrack3D: an automatic high-throughput phenotyping pipeline to track maize organs over time	5
Meta-analysis of the role of zinc in coordinating absorption of mineral elements in wheat seedlings	5
Karst vegetation coverage detection using UAV multispectral vegetation indices and machine learning algorithm	5
Soybean leaf estimation based on RGB images and machine learning methods	5
An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues	5
Research on anthracnose grade of Camellia oleifera based on the combined LIBS and THz technology	5
Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter	5
Crop insect pest detection based on dilated multi-scale attention U-Net	5
Development and application of a virus-induced gene silencing protocol for the study of gene function in narrow-leafed lupin	5
Quantification of spatial metal accumulation patterns in Noccaea caerulescens by X-ray fluorescence image processing for genetic studies	5
Plant cell wall hydrolysis process reveals structure–activity relationships	5
Effects of cushion box and closed let-down ladder usage on damage to corn during handling: physiological deterioration	5
Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants	5
A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction	5
Three-dimensional branch segmentation and phenotype extraction of maize tassel based on deep learning	5
Comparison of non-subjective relative fungal biomass measurements to quantify the Leptosphaeria maculans—Brassica napus interaction	5
A custom library construction method for super-resolution ribosome profiling in Arabidopsis	5
A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants	5
Monitoring of drought stress and transpiration rate using proximal thermal and hyperspectral imaging in an indoor automated plant phenotyping platform	5
RGB image-based method for phenotyping rust disease progress in pea leaves using R	5
Field cabbage detection and positioning system based on improved YOLOv8n	5
Improved wood species identification based on multi-view imagery of the three anatomical planes	5
Axial variation in flexural stiffness of plant stem segments: measurement methods and the influence of measurement uncertainty	5
Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study	5
A device for the controlled cooling and freezing of excised plant specimens during magnetic resonance imaging	5
Detection of reed using cnn method and analysis of the dry reed (phragmites australis) for a sustainable lake area	5
PredCRG: A computational method for recognition of plant circadian genes by employing support vector machine with Laplace kernel	5