Engineering in Life Sciences

Article	Citations
Engineering and evaluation of thermostable IsPETase variants for PET degradation	62
Recent applications of plant cell culture technology in cosmetics and foods	61
Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy	61
Aptasensors versus immunosensors—Which will prevail?	59
Enzyme immobilization in hydrogels: A perfect liaison for efficient and sustainable biocatalysis	48
Purification and characterization of the receptor‐binding domain of SARS‐CoV‐2 spike protein from Escherichia coli	42
A review of sustainable biodiesel production using biomass derived heterogeneous catalysts	38
Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum	34
Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends	32
The pro‐inflammatory response of macrophages regulated by acid degradation products of poly(lactide‐co‐glycolide) nanoparticles	30
Study on dynamic changes of microbial community and lignocellulose transformation mechanism during green waste composting	28
Nanofiltration membrane for bio‐separation: Process‐oriented materials innovation	28
Artificial microbial consortia for bioproduction processes	27
Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems—A critical review	27
Photobioreactors for cultivation and synthesis: Specifications, challenges, and perspectives	25
3D printing in biotechnology—An insight into miniaturized and microfluidic systems for applications from cell culture to bioanalytics	24
Fabrication of Au/Fe3O4/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)	24
One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles	24
Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field	19
bletl ‐ A Python package for integrating BioLector microcultivation devices in the Design‐Build‐Test‐Learn cycle	18
pyFOOMB: Python framework for object oriented modeling of bioprocesses	18
Analysis of microbial diversity and succession during Xiaoqu Baijiu fermentation using high‐throughput sequencing technology	18
Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur‐oxidizing bacteria	17
Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms	17
Assessment of extraction options for a next‐generation biofuel: Recovery of bio‐isobutanol from aqueous solutions	16

An analysis of organism lifelines in an industrial bioreactor using Lattice‐Boltzmann CFD	15
A novel downstream process for highly pure 1,3‐propanediol from an efficient fed‐batch fermentation of raw glycerol by Clostridium pasteurianum	15
New developments in biological phosphorus accessibility and recovery approaches from soil and waste streams	15
Retracted: Comparative molecular docking and molecular‐dynamic simulation of wild‐type‐ and mutant carboxylesterase with BTA‐hydrolase for enhanced binding to plastic	15
Separation and purification of plant terpenoids from biotransformation	14
Removal of sugars in wastewater from food production through heterotrophic growth of Galdieria sulphuraria	14
Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains	14
Scaling‐down biopharmaceutical production processes via a single multi‐compartment bioreactor (SMCB)	14
Vascular implants – new aspects for in situ tissue engineering	14
Interrogation of the perturbed gut microbiota in gouty arthritis patients through in silico metabolic modeling	14
A novel twin‐column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants	13
Advances in automated real‐time flow cytometry for monitoring of bioreactor processes	13
Characterization of an aerated submerged hollow fiber ultrafiltration device for efficient microalgae harvesting	12
Improvement of glycine biosynthesis from one‐carbon compounds and ammonia catalyzed by the glycine cleavage system in vitro	11
Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis	11
Micro‐aerobic production of isobutanol with engineered Pseudomonas putida	11
Optimization of a mAb production process with regard to robustness and product quality using quality by design principles	10
Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell‐based therapy products	10
Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles	10
Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring	9
Novel synthetic co‐culture of Acetobacterium woodii and Clostridium drakei using CO2 and in situ generated H2 for the production of caproic acid via lactic acid	9
Production of natural colorants by liquid fermentation with Chlorociboria aeruginascens and Laetiporus sulphureus and prospective applications	9
A worldwide survey on the use of animal‐derived materials and reagents in scientific experimentation	9
Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO2	9
Monitoring cell productivity for the production of recombinant proteins by flow cytometry: An effective application using the cold capture assay	9
Assessment of microbial activity by CO2 production during heating oil storage	9
Implementation of QbD strategies in the inoculum expansion of a mAb production process	9
Acceleration of antibacterial activity of curcumin loaded biopolymers against methicillin‐resistant Staphylococcus aureus: Synthesis, optimization, and evaluation	9
A fluorescence‐based yeast sensor for monitoring acetic acid	8
Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica	8
CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy	8
Characterization of a novel microfluidic platform for the isolation of rare single cells to enable CTC analysis from head and neck squamous cell carcinoma patients	8
Upgrading of bio‐separation and bioanalysis using synthetic polymers: Molecularly imprinted polymers (MIPs), cryogels, stimuli‐responsive polymers	7
Pilot scale isolation of exopolysaccharides from Streptococcus thermophilus DGCC7710: Impact of methodical details on macromolecular properties and technofunctionality	7
Improving carotenoid production in recombinant yeast, Saccharomyces cerevisiae, using ultrasound‐irradiated two‐phase extractive fermentation	7
Cultivated meat manufacturing: Technology, trends, and challenges	7
Preparation of lignosulfonate‐based nanofiltration membranes with improved water desalination performance	7
Parameter and state estimation of backers yeast cultivation with a gas sensor array and unscented Kalman filter	7
Assessing the growth kinetics and stoichiometry of Escherichia coli at the single‐cell level	7
Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography	6
Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form	6
Enhanced sequestration of carbon dioxide into calcium carbonate using pressure and a carbonic anhydrase from alkaliphilic Coleofasciculus chthonoplastes	6
Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system	6
Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13	6
Synthesis of ethyl acetate from glucose by Kluyveromyces marxianus, Cyberlindnera jadinii and Wickerhamomyces anomalus depending on the induction mode	6
Machine learning‐based automated fungal cell counting under a complicated background with ilastik and ImageJ	6
Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology	6
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors	6
Removal of heavy metals from industrial wastewater using microbial fuel cell	6
Implementing a digital infrastructure for the lab using a central laboratory server and the SiLA2 communication standard	5

FAIR research data management as community approach in bioengineering	5
Evaluation of the mechanical properties and clinical application of nickel–titanium shape memory alloy scaphoid arc nail	5
Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda	5
Evaluation of SDS‐coated iron nanostructure on the gene expression of bio surfactant‐producing genes by Pseudomonas aeruginosa	5
Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine	5
Stress‐induced increase of monoclonal antibody production in CHO cells	5
Facilitated endospore detection for Bacillus spp. through automated algorithm‐based image processing	5
Patterned vascularization in a directional ice‐templated scaffold of decellularized matrix	5
Synthetic mutualism in engineered E. coli mutant strains as functional basis for microbial production consortia	5
Impact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells	5
Three‐liquid‐phase salting‐out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)‐rich oils from Euphausia superba	5
Modeling multiphase fluid flow, mass transfer, and chemical reactions in bioreactors using large‐eddy simulation	5
Multi‐objective Bayesian algorithm automatically discovers low‐cost high‐growth serum‐free media for cellular agriculture application	4
In‐line monitoring of protein concentration with MIR spectroscopy during UFDF	4
Study on the development and integration of 3D‐printed optics in small‐scale productions of single‐use cultivation vessels	4
Invitro biocompatibility evaluation of a heat‐resistant 3D printing material for use in customized cell culture devices	4
Cost‐effective purification process development for chimeric hepatitis B core (HBc) virus‐like particles assisted by molecular dynamic simulation	4
A versatile ceramic capillary membrane reactor system for continuous enzyme‐catalyzed hydrolysis	4
Co‐cultivation of diazotrophic terrestrial cyanobacteria and Arabidopsis thaliana	4
Development of purification process for dual‐function recombinant human heavy‐chain ferritin by the investigation of genetic modification impact on conformation	4
A novel LysR‐type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin	4
Rapid in‐situ aerobic biodegradation of high salt and oily food waste employing constructed synthetic microbiome	4
R‐based method for quantitative analysis of biofilm thickness by using confocal laser scanning microscopy	4
Increasing immunoglobulin G adsorption in dextran‐grafted protein A gels	4
Sporosarcina pasteuriican be used to print a layer of calcium carbonate	4
Ensemble‐based adaptive soft sensor for fault‐tolerant biomass monitoring	4
Comparison of various classification techniques for supervision of milk processing	4
Application of an Escherichia coli triple reporter strain for at‐line monitoring of single‐cell physiology during L‐phenylalanine production	4
Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form	3
Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase	3
Escherichia coli aceE variants coding pyruvate dehydrogenase improve the generation of pyruvate‐derived acetoin	3
Influence of oxygen concentration on the metabolism of Penicillium chrysogenum	3
Online monitoring applying the anaerobic respiratory monitoring system reveals iron(II) limitation in YTF medium for Clostridium ljungdahlii	3
Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production	3
Gut microbiota induced epigenetic modifications in the non‐alcoholic fatty liver disease pathogenesis	3
An optimized live bacterial delivery vehicle safely and efficaciously delivers bacterially transcribed therapeutic nucleic acids	3
Applying intensified design of experiments to mammalian cell culture processes	3
Predicting the potential of capacitive deionization for the separation of pH‐dependent organic molecules	3
Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification	3
Reaction kinetics of anodic biofilms under changing substrate concentrations: Uncovering shifts in Nernst‐Monod curves via substrate pulses	3
Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression	3
Integration of a perfusion reactor and continuous precipitation in an entirely membrane‐based process for antibody capture	2
Compartment‐specific 13C metabolic flux analysis reveals boosted NADPH availability coinciding with increased cell‐specific productivity for IgG1 producing CHO cells after MTA treatment	2
A new approach for balancing the microbial synthesis of ethyl acetate and other volatile metabolites during aerobic bioreactor cultivations	2
Evaluation of restricted access media for the purification of cell culture‐derived Orf viruses	2
Monitoring the apical growth characteristics of hairy roots using non‐invasive laser speckle contrast imaging	2
Flavonoids, gut microbiota, and host lipid metabolism	2
From single‐cell cloning to high‐yield influenza virus production – implementing advanced technologies in vaccine process development	2
Robot‐based 6D bioprinting for soft tissue biomedical applications	2
Scalable downstream method for the cyclic lipopetide jagaricin	2
Biocompatible scaffolds based on collagen and oxidized dextran for endothelial cell survival and function in tissue engineering	2
Diffusion profiles in L. lactis biofilms under different conditions	2
Flexible biofilm monitoring device	2
Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures	2
Identification and characterization of inulinases by bioinformatics analysis of bacterial glycoside hydrolases family 32 (GH32)	2
Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate	2
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold	2
Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system	2
Realization of user‐friendly bioanalytical tools to quantify and monitor critical components in bio‐industrial processes through conceptual design	2
Cell‐free synthesis of silver nanoparticles in spent media of different Aspergillus species	2
Yeast autonomously replicating sequence (ARS): Identification, function, and modification	2
Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed‐batch (CFB) mode	1
Inside Back Cover: Co‐cultivation of diazotrophic terrestrial cyanobacteria and Arabidopsis thaliana	1
Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions	1
Back Cover: Recent applications of plant cell culture technology in cosmetics and foods	1
Design of cell expansion processes for adherent‐growing cells with mDoE‐workflow	1
Salting‐out extraction of recombinant κ‐carrageenase and phage T7 released fromEscherichia colicells	1
Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells	1
Micro flow photochemical synthesis of Ca‐sensitive fluorescent sensor particles	1
Application of Artificial Neural Network for predicting biomass growth during domestic wastewater treatment through a biological process	1
Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody	1
Current trends in medium‐chain‐length polyhydroxyalkanoates: Microbial production, purification, and characterization	1
A novel approach for enumeration of extracellular vesicles from crude and purified cell culture samples	1
Meta‐omics assisted microbial gene and strain resources mining in contaminant environment	1
Investigation and evaluation of a 3D‐printed optical modified cultivation vessel for improved scattered light measurement of biotechnologically relevant organisms	1
The cultivation conditions affect the aggregation and functionality of β‐cell lines alone and in coculture with mesenchymal stromal/stem cells	1
Front Cover: Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems—A critical review	1
Design of a synthesis‐friendly hypoxia‐responsive promoter for cell‐based therapeutics	1
Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement	1
Dynamic energy correlation analysis of E. coli aspartokinase III and alteration of allosteric regulation by manipulating energy transduction pathways	1

Site‐directed mutagenesis improves the practical application of L‐glutamic acid decarboxylase inEscherichia coli	1
Endospore production of Bacillus spp. for industrial use	1
Membrane inlet—ion mobility spectrometry with automatic spectra evaluation as online monitoring tool for the process control of microalgae cultivation	1
Real‐time droplet size analysis using laser micrometer as a process analytical technology tool for continuous dripping process	1
Efficacy of minute virus of mice (MVM) inactivation utilizing high temperature short time (HTST) pasteurization and suitability assessment of pasteurized, concentrated glucose feeds in Chinese hamster	1
Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives’ detection capabilities	1
Mobile SARS‑CoV‑2 screening facilities for rapid deployment and university‐based diagnostic laboratory	1
Genomic markers on synthetic genomes	1
Gas supply apparatus using rotational motion of shaking incubator for flask culture of aerobic microorganisms	1
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Downstream bioprocessing of human pluripotent stem cell‐derived therapeutics	0
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Cover Feature: Parameter and state estimation of backers yeast cultivation with a gas sensor array and unscented Kalman filter	0
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Evaluation of prokaryotic and eukaryotic microbial communities on microplastic‐associated biofilms in marine and freshwater environments	0
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Identification of Plant Peroxidases Catalyzing the Degradation of Fluorinated Aromatics Using a Peroxidase Library Approach	0
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Generation and evaluation of input values for computational analysis of transport processes within tissue cultures	0
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Downstream processing of bioproducts	0
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Inside Front Cover: pyFOOMB: Python framework for object oriented modeling of bioprocesses	0
Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry – A Review	0
Cover Feature: Regulation of pyruvate dehydrogenase complex related to lactate switch in CHO cells	0
Exploitation of the ribosomal protein L10 R98S mutation to enhance recombinant protein production in mammalian cells	0
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In situ Product Recovery of Microbially Synthesized Ethyl Acetate from the Exhaust Gas of a Bioreactor by Membrane Technology	0
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SIGHT—A System for Solvent‐Tight Incubation and Growth Monitoring in High Throughput	0
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An account on the innovative path of cooperation with Prof. Zhiguo Su in bioseparation	0
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Phenotype analysis of cultivation processes via unsupervised machine learning: Demonstration for Clostridium pasteurianum	0
Developing a surface acoustic wave‐induced microfluidic cell lysis device for point‐of‐care DNA amplification	0
A perspective‐driven and technical evaluation of machine learning in bioreactor scale‐up: A case‐study for potential model developments	0
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3D‐printed autoclavable plant holders to facilitate large‐scale protein production in plants	0
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Rapid exometabolome footprinting combined with multivariate statistics: A powerful tool for bioprocess optimization	0
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Stable overexpression of native and artificial miRNAs for the production of differentially fucosylated antibodies in CHO cells	0
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Online deployment of an O‐PLS model for dielectric spectroscopy‐based inline monitoring of viable cell concentrations in Chinese hamster ovary cell perfusion cultivations	0
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Automized inline monitoring in perfused mammalian cell culture by MIR spectroscopy without calibration model building	0
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Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium	0
Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency	0
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