Engineering in Life Sciences

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Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations	59
Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field	51
Application of Artificial Neural Network for predicting biomass growth during domestic wastewater treatment through a biological process	46
Flavonoids, gut microbiota, and host lipid metabolism	42
Application of an Escherichia coli triple reporter strain for at‐line monitoring of single‐cell physiology during L‐phenylalanine production	41
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An optimized live bacterial delivery vehicle safely and efficaciously delivers bacterially transcribed therapeutic nucleic acids	26
Salting‐out extraction of recombinant κ‐carrageenase and phage T7 released fromEscherichia colicells	26
Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms	24
Magnetizing Biotech–Advances in (In Vivo) Magnetic Enzyme Immobilization	23
Automation of a Capillary‐Wave Microbioreactor Platform to Enhance Phage Sensitivity Screen Efficiency	21
Comparison of various classification techniques for supervision of milk processing	20
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Downstream bioprocessing of human pluripotent stem cell‐derived therapeutics	18
Cover Picture: Engineering in Life Sciences 3'23	17
RETRACTION: Evaluation of SDS‐Coated Iron Nanostructure on the Gene Expression of Bio Surfactant‐Producing Genes by Pseudomonas Aeruginosa	17

Ensemble‐based adaptive soft sensor for fault‐tolerant biomass monitoring	17
Modeling multiphase fluid flow, mass transfer, and chemical reactions in bioreactors using large‐eddy simulation	16
Reaction kinetics of anodic biofilms under changing substrate concentrations: Uncovering shifts in Nernst‐Monod curves via substrate pulses	16
Evaluating microbial contaminations of alternative heating oils	15
In‐line monitoring of protein concentration with MIR spectroscopy during UFDF	15
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Efficacy of minute virus of mice (MVM) inactivation utilizing high temperature short time (HTST) pasteurization and suitability assessment of pasteurized, concentrated glucose feeds in Chinese hamster	14
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Cover Picture: Engineering in Life Sciences 4'24	13
Genetic modules for α‐factor pheromone controlled growth regulation of Saccharomyces cerevisiae	13
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Reaction Engineering for Asymmetric R‐/S‐PAC Synthesis With Ephedrine or Pseudoephedrine Dehydrogenase in Pickering Emulsion	13
Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase	12
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bletl ‐ A Python package for integrating BioLector microcultivation devices in the Design‐Build‐Test‐Learn cycle	12
Biological recovery of phosphorus (BioP‐Rec) from wastewater streams using brewer's yeast on pilot‐scale	12
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Identification and characterization of inulinases by bioinformatics analysis of bacterial glycoside hydrolases family 32 (GH32)	10
Real‐Time VCC Monitoring and Forecasting in HEK‐Cell‐Based rAAV Vector Production Using Capacitance Spectroscopy	10
Corrigendum	10
Advances in automated real‐time flow cytometry for monitoring of bioreactor processes	10
Impact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells	10
Editing Streptomyces genome using target AID system fused with UGI‐degradation tag	10
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Evaluating scaling of capillary photo‐biofilm reactors for high cell density cultivation of mixed trophies artificial microbial consortia	9
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Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum	8
Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form	8
Influenza A Virus Production Following Quality by Design Principles	8
Novel synthetic co‐culture of Acetobacterium woodii and Clostridium drakei using CO2 and in situ generated H2 for the production of caproic acid via lactic acid	8
Meet Our Editorial Board—Engineering in Life Sciences. An Interview with Antonina “Tonya” Lavrentieva, Leibniz Universität Hannover, Institut für Technische Chemie, Hannover, Germany	8
Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody	8
Regulation on C2‐C8 carboxylic acid biosynthesis from anaerobic CO2 fermentation	8
Cover Picture: Engineering in Life Sciences 6'21	7
Micro flow photochemical synthesis of Ca‐sensitive fluorescent sensor particles	7
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Aptasensors versus immunosensors—Which will prevail?	7
3D‐printed autoclavable plant holders to facilitate large‐scale protein production in plants	7
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Secretory Production of Heterologous Antimicrobial Peptides in Corynebacterium glutamicum	7
Generation and evaluation of input values for computational analysis of transport processes within tissue cultures	6
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Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures	6
Gas supply apparatus using rotational motion of shaking incubator for flask culture of aerobic microorganisms	6
Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring	6
Online deployment of an O‐PLS model for dielectric spectroscopy‐based inline monitoring of viable cell concentrations in Chinese hamster ovary cell perfusion cultivations	6
Mechanical Microvibration Device Enhancing Immunohistochemistry Efficiency	6
Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy	5
Patterned vascularization in a directional ice‐templated scaffold of decellularized matrix	5
Machine learning‐based automated fungal cell counting under a complicated background with ilastik and ImageJ	5

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Flexible biofilm monitoring device	5
Study on the development and integration of 3D‐printed optics in small‐scale productions of single‐use cultivation vessels	5
Cell‐free synthesis of silver nanoparticles in spent media of different Aspergillus species	5
Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing	5
Stable overexpression of native and artificial miRNAs for the production of differentially fucosylated antibodies in CHO cells	5
Evaluation of SDS‐coated iron nanostructure on the gene expression of bio surfactant‐producing genes by Pseudomonas aeruginosa	5
Production of an Innovative, Surface Area‐Enhanced and Biodegradable Biofilm‐Generating Device by 3D Printing	5
Optimization of a mAb production process with regard to robustness and product quality using quality by design principles	5
Evaluation of Yeast Alcohol Acetyltransferases for Ethyl Acetate Production in Clostridium ljungdahlii	5
Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium	4
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Engineering Caffeic Acid O‐Methyltransferase for Efficient De Novo Ferulic Acid Synthesis	4
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Three‐liquid‐phase salting‐out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)‐rich oils from Euphausia superba	4
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Cultivated meat manufacturing: Technology, trends, and challenges	4
Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production	4
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Cracks Repairing and Resistance to Water Penetration Properties of Microbial Self‐Healing Cement	4
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Meta‐omics assisted microbial gene and strain resources mining in contaminant environment	3
Artificial microbial consortia for bioproduction processes	3
Cover Picture: Engineering in Life Sciences 1'23	3
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Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions	3
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Integration of a perfusion reactor and continuous precipitation in an entirely membrane‐based process for antibody capture	3
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Site‐directed mutagenesis improves the practical application of L‐glutamic acid decarboxylase inEscherichia coli	2
Synthetic mutualism in engineered E. coli mutant strains as functional basis for microbial production consortia	2
Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement	2
Meet Our Editorial Board—Engineering in Life Sciences. An Interview With Michael Zavrel	2
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Employing QbD strategies to assess the impact of cell viability and density on the primary recovery of monoclonal antibodies	2
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Overproduction and Characterization of Recombinant Soluble Trypanosoma brucei Phospholipase A2	2
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Acceleration of antibacterial activity of curcumin loaded biopolymers against methicillin‐resistant Staphylococcus aureus: Synthesis, optimization, and evaluation	2
From single‐cell cloning to high‐yield influenza virus production – implementing advanced technologies in vaccine process development	2
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Removal of heavy metals from industrial wastewater using microbial fuel cell	2
Cover Picture: Engineering in Life Sciences 7'24	1
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold	1
3D printing in biotechnology—An insight into miniaturized and microfluidic systems for applications from cell culture to bioanalytics	1
Automized inline monitoring in perfused mammalian cell culture by MIR spectroscopy without calibration model building	1
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Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis	1
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Cover Picture: Engineering in Life Sciences 9'24	1
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Influence of oxygen concentration on the metabolism of Penicillium chrysogenum	1
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Design of cell expansion processes for adherent‐growing cells with mDoE‐workflow	1
Designing Smartly: Understanding the Crystallinity of Melt Electrowritten Scaffolds	1
Cover Picture: Engineering in Life Sciences 8–9'21	1
Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification	1
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Upgrading of bio‐separation and bioanalysis using synthetic polymers: Molecularly imprinted polymers (MIPs), cryogels, stimuli‐responsive polymers	1
Rapid exometabolome footprinting combined with multivariate statistics: A powerful tool for bioprocess optimization	1
Characterization of an aerated submerged hollow fiber ultrafiltration device for efficient microalgae harvesting	1
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Evaluation of restricted access media for the purification of cell culture‐derived Orf viruses	0
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Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry – A Review	0
Applying intensified design of experiments to mammalian cell culture processes	0

Separation and purification of plant terpenoids from biotransformation	0
Implementation of ubiquitous chromatin opening elements as artificial integration sites for CRISPR/Cas9‐mediated knock‐in in mammalian cells	0
Optimizations of Placenta Extracellular Matrix‐Loaded Silk Fibroin/Alginate 3D‐Printed Scaffolds Structurally and Functionally for Bone Tissue Engineering	0
Biocompatible scaffolds based on collagen and oxidized dextran for endothelial cell survival and function in tissue engineering	0
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Microbiome dynamics and products profiles of biowaste fermentation under different organic loads and additives	0
The pro‐inflammatory response of macrophages regulated by acid degradation products of poly(lactide‐co‐glycolide) nanoparticles	0
FAIR research data management as community approach in bioengineering	0
Choline‐Based Deep Eutectic Solvents for Enzymatic Preparation of Epoxy Linseed Oil	0
SIGHT—A System for Solvent‐Tight Incubation and Growth Monitoring in High Throughput	0
A novel downstream process for highly pure 1,3‐propanediol from an efficient fed‐batch fermentation of raw glycerol by Clostridium pasteurianum	0
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Scalable downstream method for the cyclic lipopetide jagaricin	0
Assessment of microbial activity by CO2 production during heating oil storage	0
Digitalization concepts in academic bioprocess development	0
Assessment of extraction options for a next‐generation biofuel: Recovery of bio‐isobutanol from aqueous solutions	0
Phenotype analysis of cultivation processes via unsupervised machine learning: Demonstration for Clostridium pasteurianum	0
Microbiome research for advancing engineering in life science	0
Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology	0
Predicting the potential of capacitive deionization for the separation of pH‐dependent organic molecules	0
Special issue for Thomas Scheper's retirement	0
Realization of user‐friendly bioanalytical tools to quantify and monitor critical components in bio‐industrial processes through conceptual design	0
Cover Picture: Engineering in Life Sciences 2'24	0
Endospore production of Bacillus spp. for industrial use	0
Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives’ detection capabilities	0
A novel twin‐column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants	0
Cover Picture: Engineering in Life Sciences 7'23	0
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Fabrication of Au/Fe3O4/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)	0
Analysis of microbial diversity and succession during Xiaoqu Baijiu fermentation using high‐throughput sequencing technology	0
Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles	0
Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13	0
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Cover Picture: Engineering in Life Sciences 6'22	0
Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur‐oxidizing bacteria	0
Multi‐objective Bayesian algorithm automatically discovers low‐cost high‐growth serum‐free media for cellular agriculture application	0
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A worldwide survey on the use of animal‐derived materials and reagents in scientific experimentation	0
Current trends in medium‐chain‐length polyhydroxyalkanoates: Microbial production, purification, and characterization	0
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Genomic markers on synthetic genomes	0
Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica	0
Real‐time droplet size analysis using laser micrometer as a process analytical technology tool for continuous dripping process	0
Cover Picture: Engineering in Life Sciences 10'22	0
Identification of Plant Peroxidases Catalyzing the Degradation of Fluorinated Aromatics Using a Peroxidase Library Approach	0
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Yeast autonomously replicating sequence (ARS): Identification, function, and modification	0
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Assessing the growth kinetics and stoichiometry of Escherichia coli at the single‐cell level	0
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Rheology and Culture Reproducibility of Filamentous Microorganisms: Impact of Flow Behavior and Oxygen Transfer During Salt‐Enhanced Cultivation of the Actinomycete Actinomadura namibiensis	0
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Cover Picture: Engineering in Life Sciences 7'22	0
Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda	0
Photobioreactors for cultivation and synthesis: Specifications, challenges, and perspectives	0
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Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system	0
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Enzyme immobilization in hydrogels: A perfect liaison for efficient and sustainable biocatalysis	0
Mobile SARS‑CoV‑2 screening facilities for rapid deployment and university‐based diagnostic laboratory	0
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Cover Picture: Engineering in Life Sciences 12'22	0
Mini Bubble Columns for Miniaturizing Scale‐Down	0
An analysis of organism lifelines in an industrial bioreactor using Lattice‐Boltzmann CFD	0
An account on the innovative path of cooperation with Prof. Zhiguo Su in bioseparation	0
A review of sustainable biodiesel production using biomass derived heterogeneous catalysts	0
Raman‐Enabled Predictions of Protein Content and Metabolites in Biopharmaceutical Saccharomyces cerevisiae Fermentations	0
Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system	0
Robot‐based 6D bioprinting for soft tissue biomedical applications	0
Compartment‐specific 13C metabolic flux analysis reveals boosted NADPH availability coinciding with increased cell‐specific productivity for IgG1 producing CHO cells after MTA treatment	0
Investigation and evaluation of a 3D‐printed optical modified cultivation vessel for improved scattered light measurement of biotechnologically relevant organisms	0
Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate	0
Gut microbiota induced epigenetic modifications in the non‐alcoholic fatty liver disease pathogenesis	0
One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles	0
Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed‐batch (CFB) mode	0
Developing a surface acoustic wave‐induced microfluidic cell lysis device for point‐of‐care DNA amplification	0
Interrogation of the perturbed gut microbiota in gouty arthritis patients through in silico metabolic modeling	0
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Membrane inlet—ion mobility spectrometry with automatic spectra evaluation as online monitoring tool for the process control of microalgae cultivation	0
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Combination of Two‐Stage Continuous Feeding and Optimized Synthetic Medium Increases Lipid Production in Lipomyces starkeyi	0
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Improving carotenoid production in recombinant yeast, Saccharomyces cerevisiae, using ultrasound‐irradiated two‐phase extractive fermentation	0
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Engineering and evaluation of thermostable IsPETase variants for PET degradation	0
Rapid in‐situ aerobic biodegradation of high salt and oily food waste employing constructed synthetic microbiome	0
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