Engineering in Life Sciences

Article	Citations
	858927920
Preparation of a pH‐responsive chitosan‐montmorillonite‐nitrogen‐doped carbon quantum dots nanocarrier for attenuating doxorubicin limitations in cancer therapy	50
Engineering and evaluation of thermostable IsPETase variants for PET degradation	50
Aptasensors versus immunosensors—Which will prevail?	49
Recent applications of plant cell culture technology in cosmetics and foods	47
Biocatalytic membrane: Go far beyond enzyme immobilization	40
Purification and characterization of the receptor‐binding domain of SARS‐CoV‐2 spike protein from Escherichia coli	38
Enzyme immobilization in hydrogels: A perfect liaison for efficient and sustainable biocatalysis	37
Ultra pH‐sensitive detection of total and free prostate‐specific antigen using electrochemical aptasensor based on reduced graphene oxide/gold nanoparticles emphasis on TiO2/carbon quantum	28
Investigation of a two‐step device implementing magnetophoresis and dielectrophoresis for separation of circulating tumor cells from blood cells	27
A review of sustainable biodiesel production using biomass derived heterogeneous catalysts	24
Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems—A critical review	23
Continuous capture of recombinant antibodies by ZnCl2 precipitation without polyethylene glycol	23
The pro‐inflammatory response of macrophages regulated by acid degradation products of poly(lactide‐co‐glycolide) nanoparticles	22
Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends	22
Study on dynamic changes of microbial community and lignocellulose transformation mechanism during green waste composting	21
Production of β‐carotene with Dunaliella salina CCAP19/18 at physically simulated outdoor conditions	21
Nanofiltration membrane for bio‐separation: Process‐oriented materials innovation	20
Anti‐EGFR antibody‐drug conjugate for triple‐negative breast cancer therapy	20
One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles	20
Fabrication of Au/Fe3O4/RGO based aptasensor for measurement of miRNA‐128, a biomarker for acute lymphoblastic leukemia (ALL)	18
3D printing in biotechnology—An insight into miniaturized and microfluidic systems for applications from cell culture to bioanalytics	17
bletl ‐ A Python package for integrating BioLector microcultivation devices in the Design‐Build‐Test‐Learn cycle	16
Photobioreactors for cultivation and synthesis: Specifications, challenges, and perspectives	16
Tissue engineering in female pelvic floor reconstruction	16

Efficient phenol degradation by laccase immobilized on functional magnetic nanoparticles in fixed bed reactor under high‐gradient magnetic field	15
pyFOOMB: Python framework for object oriented modeling of bioprocesses	15
Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains	14
Artificial microbial consortia for bioproduction processes	14
Kinetic characteristics of long‐term repeated fed‐batch (LtRFb) l‐lactic acid fermentation by a Bacillus coagulans strain	13
An analysis of organism lifelines in an industrial bioreactor using Lattice‐Boltzmann CFD	12
Removal of sugars in wastewater from food production through heterotrophic growth of Galdieria sulphuraria	12
Assessment of extraction options for a next‐generation biofuel: Recovery of bio‐isobutanol from aqueous solutions	12
Recent advances in microbial capture of hydrogen sulfide from sour gas via sulfur‐oxidizing bacteria	12
Interrogation of the perturbed gut microbiota in gouty arthritis patients through in silico metabolic modeling	12
A novel downstream process for highly pure 1,3‐propanediol from an efficient fed‐batch fermentation of raw glycerol by Clostridium pasteurianum	11
Collective cell migration of fibroblasts is affected by horizontal vibration of the cell culture dish	11
Characterization of an aerated submerged hollow fiber ultrafiltration device for efficient microalgae harvesting	11
Vascular implants – new aspects for in situ tissue engineering	11
Scaling‐down biopharmaceutical production processes via a single multi‐compartment bioreactor (SMCB)	11
New developments in biological phosphorus accessibility and recovery approaches from soil and waste streams	11
Improvement of glycine biosynthesis from one‐carbon compounds and ammonia catalyzed by the glycine cleavage system in vitro	11
Advances in automated real‐time flow cytometry for monitoring of bioreactor processes	11
Analysis of microbial diversity and succession during Xiaoqu Baijiu fermentation using high‐throughput sequencing technology	11
Effects and interactions of metal oxides in microparticle‐enhanced cultivation of filamentous microorganisms	11
Characterization and stabilization in process development and product formulation for super large proteinaceous particles	10
A novel twin‐column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants	10
Separation and purification of plant terpenoids from biotransformation	10
Manipulation of pore structure during manufacture of agarose microspheres for bioseparation	10
Implementation of QbD strategies in the inoculum expansion of a mAb production process	9
Development of a 3D‐printed single‐use separation chamber for use in mRNA‐based vaccine production with magnetic microparticles	9
Preparation and evaluation of amphipathic lipopeptide‐loaded PLGA microspheres as sustained‐release system for AIDS prevention	9
A worldwide survey on the use of animal‐derived materials and reagents in scientific experimentation	9
Elaboration of an algae‐to‐energy system and recovery of water and nutrients from municipal sewage	9
Micro‐aerobic production of isobutanol with engineered Pseudomonas putida	9
Production of natural colorants by liquid fermentation with Chlorociboria aeruginascens and Laetiporus sulphureus and prospective applications	8
Effect of mesoporous silica nanoparticles‐based nano‐fragrance on the central nervous system	8
Optimization of a mAb production process with regard to robustness and product quality using quality by design principles	8
Retracted: Comparative molecular docking and molecular‐dynamic simulation of wild‐type‐ and mutant carboxylesterase with BTA‐hydrolase for enhanced binding to plastic	8
Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring	8
Immobilization of an amino acid racemase for application in crystallization‐based chiral resolutions of asparagine monohydrate	7
A fluorescence‐based yeast sensor for monitoring acetic acid	7
Characterization of a novel microfluidic platform for the isolation of rare single cells to enable CTC analysis from head and neck squamous cell carcinoma patients	7
Purification of a peptide tagged protein via an affinity chromatographic process with underivatized silica	7
Monitoring cell productivity for the production of recombinant proteins by flow cytometry: An effective application using the cold capture assay	7
Novel synthetic co‐culture of Acetobacterium woodii and Clostridium drakei using CO2 and in situ generated H2 for the production of caproic acid via lactic acid	7
Development and characterization of a fiber optical fluorescence sensor for the online monitoring of biofilms and their microenvironment	7
Influence of pH value and salts on the adsorption of lysozyme in mixed‐mode chromatography	6
Physiologic isolation and expansion of human mesenchymal stem/stromal cells for manufacturing of cell‐based therapy products	6
Assessing the growth kinetics and stoichiometry of Escherichia coli at the single‐cell level	6
Pilot scale isolation of exopolysaccharides from Streptococcus thermophilus DGCC7710: Impact of methodical details on macromolecular properties and technofunctionality	6
CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy	6
Parameter and state estimation of backers yeast cultivation with a gas sensor array and unscented Kalman filter	6
Investigation of cell line specific responses to pH inhomogeneity and consequences for process design	6
Removal of heavy metals from industrial wastewater using microbial fuel cell	6

Streamlining of a synthetic co‐culture towards an individually controllable one‐pot process for polyhydroxyalkanoate production from light and CO2	6
Synthesis of ethyl acetate from glucose by Kluyveromyces marxianus, Cyberlindnera jadinii and Wickerhamomyces anomalus depending on the induction mode	6
Acceleration of antibacterial activity of curcumin loaded biopolymers against methicillin‐resistant Staphylococcus aureus: Synthesis, optimization, and evaluation	6
High bioreactor production and emulsifying activity of an unusual exopolymer by Chromohalobacter canadensis 28	5
Evaluation of SDS‐coated iron nanostructure on the gene expression of bio surfactant‐producing genes by Pseudomonas aeruginosa	5
Improving carotenoid production in recombinant yeast, Saccharomyces cerevisiae, using ultrasound‐irradiated two‐phase extractive fermentation	5
A vegetable oil–based biopesticide with ovicidal activity against the two‐spotted spider mite, Tetranychus urticae Koch	5
Upgrading of bio‐separation and bioanalysis using synthetic polymers: Molecularly imprinted polymers (MIPs), cryogels, stimuli‐responsive polymers	5
Effect of metals on the regulation of acidogenic metabolism enhancing biohydrogen and carboxylic acids production from brewery spent grains: Microbial dynamics and biochemical analysis	5
Comparison of l‐tyrosine containing dipeptides reveals maximum ATP availability for l‐prolyl‐l‐tyrosine in CHO cells	5
Process adapted calibration improves fluorometric pH sensor precision in sophisticated fermentation processes	5
Machine learning‐based automated fungal cell counting under a complicated background with ilastik and ImageJ	5
Evaluation of the mechanical properties and clinical application of nickel–titanium shape memory alloy scaphoid arc nail	5
Surfactin production in the bioreactor: Emphasis on magnetic nanoparticles application	5
Pseudomonas taiwanensis biofilms for continuous conversion of cyclohexanone in drip flow and rotating bed reactors	5
Enrichment of the rebaudioside A concentration in Stevia rebaudiana extract with cyclodextrin glycosyltransferase from Bacillus licheniformis DSM 13	5
Preparation of lignosulfonate‐based nanofiltration membranes with improved water desalination performance	5
Development, characterization, and application of a 2‐Compartment system to investigate the impact of pH inhomogeneities in large‐scale CHO‐based processes	5
Regulation of pyruvate dehydrogenase complex related to lactate switch in CHO cells	5
Impact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells	4
Expression of recombinant classical swine fever virus E2 glycoprotein by endogenous Txnip promoter in stable transgenic CHO cells	4
Implementing a digital infrastructure for the lab using a central laboratory server and the SiLA2 communication standard	4
Bcl‐xL expression following articular cartilage injury and its effects on the biological function of chondrocytes	4
Efficient co‐production of propionic acid and succinic acid by Propionibacterium acidipropionici using membrane separation coupled technology	4
Study on the development and integration of 3D‐printed optics in small‐scale productions of single‐use cultivation vessels	4
Application of an Escherichia coli triple reporter strain for at‐line monitoring of single‐cell physiology during L‐phenylalanine production	4
Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system	4
Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form	4
Patterned vascularization in a directional ice‐templated scaffold of decellularized matrix	4
Enhanced sequestration of carbon dioxide into calcium carbonate using pressure and a carbonic anhydrase from alkaliphilic Coleofasciculus chthonoplastes	4
Applying intensified design of experiments to mammalian cell culture processes	3
FAIR research data management as community approach in bioengineering	3
Stress‐induced increase of monoclonal antibody production in CHO cells	3
Predicting the potential of capacitive deionization for the separation of pH‐dependent organic molecules	3
Three‐liquid‐phase salting‐out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)‐rich oils from Euphausia superba	3
Comparison of various classification techniques for supervision of milk processing	3
In‐line monitoring of protein concentration with MIR spectroscopy during UFDF	3
R‐based method for quantitative analysis of biofilm thickness by using confocal laser scanning microscopy	3
Assessment of microbial activity by CO2 production during heating oil storage	3
Co‐cultivation of diazotrophic terrestrial cyanobacteria and Arabidopsis thaliana	3
Coupling the fermentation and membrane separation process for polyamides monomer cadaverine production from feedstock lysine	3
Development of purification process for dual‐function recombinant human heavy‐chain ferritin by the investigation of genetic modification impact on conformation	3
Facilitated endospore detection for Bacillus spp. through automated algorithm‐based image processing	3
Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda	3
Ensemble‐based adaptive soft sensor for fault‐tolerant biomass monitoring	3
A novel LysR‐type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin	3
Invitro biocompatibility evaluation of a heat‐resistant 3D printing material for use in customized cell culture devices	3
Cost‐effective purification process development for chimeric hepatitis B core (HBc) virus‐like particles assisted by molecular dynamic simulation	3
Increasing immunoglobulin G adsorption in dextran‐grafted protein A gels	3
Microflow system promotes acetaminophen crystal nucleation	3
Green preparation of hydrogel particles‐in‐emulsions for simultaneous enhancement of humoral and cell‐mediated immunity	3
Online monitoring applying the anaerobic respiratory monitoring system reveals iron(II) limitation in YTF medium for Clostridium ljungdahlii	3
Chitosan‐based nanofragrance with antibacterial function applied to wallpaper	3
Modeling multiphase fluid flow, mass transfer, and chemical reactions in bioreactors using large‐eddy simulation	3
Agglomeration behaviour of magnetic microparticles during separation and recycling processes in mRNA purification	3
Sporosarcina pasteurii can be used to print a layer of calcium carbonate	3
A versatile ceramic capillary membrane reactor system for continuous enzyme‐catalyzed hydrolysis	3
Performance evaluation of a high‐throughput separation system for circulating tumor cells based on microcavity array	2
Assessing the combined effect of surface topography and substrate rigidity in human bone marrow stem cell cultures	2
Enhancement of extracellular bispecific anti‐MUC1 nanobody expression in E. coli BL21 (DE3) by optimization of temperature and carbon sources through an autoinduction condition	2
Scalable downstream method for the cyclic lipopetide jagaricin	2
Large‐scale production, purification, and function of a tumor multi‐epitope vaccine: Peptibody with bFGF/VEGFA	2
Compartment‐specific 13C metabolic flux analysis reveals boosted NADPH availability coinciding with increased cell‐specific productivity for IgG1 producing CHO cells after MTA treatment	2
Reaction kinetics of anodic biofilms under changing substrate concentrations: Uncovering shifts in Nernst‐Monod curves via substrate pulses	2
Monitoring the apical growth characteristics of hairy roots using non‐invasive laser speckle contrast imaging	2
An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system	2
Multi‐objective Bayesian algorithm automatically discovers low‐cost high‐growth serum‐free media for cellular agriculture application	2
A novel model to culture cells from giant cell tumor of bone using three‐dimensional (3D) polycaprolactone scaffold	2
Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase	2
Influence of oxygen concentration on the metabolism of Penicillium chrysogenum	2
Cell‐free synthesis of silver nanoparticles in spent media of different Aspergillus species	2
Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression	2
Novel bioreactor internals for the cultivation of spore‐forming fungi in pellet form	2
Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production	2
Issue Information	2
A new approach for balancing the microbial synthesis of ethyl acetate and other volatile metabolites during aerobic bioreactor cultivations	2
Diffusion profiles in L. lactis biofilms under different conditions	2
Gut microbiota induced epigenetic modifications in the non‐alcoholic fatty liver disease pathogenesis	1
Yeast autonomously replicating sequence (ARS): Identification, function, and modification	1
Evaluation of restricted access media for the purification of cell culture‐derived Orf viruses	1

Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate	1
Flexible biofilm monitoring device	1
Membrane inlet—ion mobility spectrometry with automatic spectra evaluation as online monitoring tool for the process control of microalgae cultivation	1
Front Cover: Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems—A critical review	1
Investigation and evaluation of a 3D‐printed optical modified cultivation vessel for improved scattered light measurement of biotechnologically relevant organisms	1
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Application of Artificial Neural Network for predicting biomass growth during domestic wastewater treatment through a biological process	1
Design of cell expansion processes for adherent‐growing cells with mDoE‐workflow	1
Simultaneous solid–liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting‐out extraction system	1
The cultivation conditions affect the aggregation and functionality of β‐cell lines alone and in coculture with mesenchymal stromal/stem cells	1
Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed‐batch (CFB) mode	1
Escherichia coli aceE variants coding pyruvate dehydrogenase improve the generation of pyruvate‐derived acetoin	1
Novel method enabling a rapid vitality determination of cyanobacteria	1
Biospeckle‐characterization of hairy root cultures using laser speckle photometry	1
Integration of a perfusion reactor and continuous precipitation in an entirely membrane‐based process for antibody capture	1
Design of a synthesis‐friendly hypoxia‐responsive promoter for cell‐based therapeutics	1
Using the inner membrane of Escherichia coli as a scaffold to anchor enzymes for metabolic flux enhancement	1
Micro flow photochemical synthesis of Ca‐sensitive fluorescent sensor particles	1
Rapid and quantitative detection of Shiga toxin1 and Shiga toxin2 based on multiple targets UPT‐LF assay	1
Rapid in‐situ aerobic biodegradation of high salt and oily food waste employing constructed synthetic microbiome	1
Realization of user‐friendly bioanalytical tools to quantify and monitor critical components in bio‐industrial processes through conceptual design	1
Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives’ detection capabilities	1
Inside Back Cover: Co‐cultivation of diazotrophic terrestrial cyanobacteria and Arabidopsis thaliana	1
An optimized live bacterial delivery vehicle safely and efficaciously delivers bacterially transcribed therapeutic nucleic acids	1
A novel approach for enumeration of extracellular vesicles from crude and purified cell culture samples	1
Mobile SARS‑CoV‑2 screening facilities for rapid deployment and university‐based diagnostic laboratory	1
Real‐time droplet size analysis using laser micrometer as a process analytical technology tool for continuous dripping process	1
Synthetic mutualism in engineered E. coli mutant strains as functional basis for microbial production consortia	1
Dynamic energy correlation analysis of E. coli aspartokinase III and alteration of allosteric regulation by manipulating energy transduction pathways	1
Efficacy of minute virus of mice (MVM) inactivation utilizing high temperature short time (HTST) pasteurization and suitability assessment of pasteurized, concentrated glucose feeds in Chinese hamster	1
Cultivated meat manufacturing: Technology, trends, and challenges	1