OOIR: Observatory of International Research

Papers

(The median citation count of BMC Biotechnology is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)

Article	Citations
Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology	72
Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion	54
Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide	42
3D printing of Ceffe-infused scaffolds for tailored nipple-like cartilage development	33
Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins	33
Parabacteroides distasonis promotes CXCL9 secretion of tumor-associated macrophages and enhances CD8+T cell activity to trigger anti-tumor immunity against anti-PD-1 treatment in non-small cell lung c	33
Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri	31
Optimized zymogram protocol from 3D spheroid cultures to study MMP-2 and -9 activities in tumor cells	28
Synergistic effect of zinc oxide-cinnamic acid nanoparticles for wound healing management: in vitro and zebrafish model studies	28
Biosafety and pharmacokinetic characteristics of polyethylene pyrrolidone modified nano selenium in rats	27
Maximizing lipid accumulation in Tetradesmus obliquus under heavy metal stress for sustainable biodiesel innovation	27
The mechanism of degradation of alizarin red by a white-rot fungus Trametes gibbosa	23
Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize	23
Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol	23
Biocompatibility and potential anticancer activity of gadolinium oxide (Gd2O3) nanoparticles against nasal squamous cell carcinoma	22
Lentiviral CRISPR-guided RNA library screening identified Adam17 as an upstream negative regulator of Procr in mammary epithelium	21
Highly multiplexed targeted sequencing strategy for infectious disease surveillance	21
Electrospun PCL membranes for localized drug delivery and bone regeneration	18
Transgenic Arabidopsis thaliana plants expressing bacterial γ-hexachlorocyclohexane dehydrochlorinase LinA	17
Optimization of lipase production using fungal isolates from oily residues	17
Fabrication and characterization of metformin-loaded PLGA/Collagen nanofibers for modulation of macrophage polarization for tissue engineering and regenerative medicine	17
Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies	16
Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves	16
Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein	16
Recombinant laccase rPOXA 1B real-time, accelerated and molecular dynamics stability study	15

Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa	15
Chitosan nano-formulation enhances stability and bactericidal activity of the lytic phage HK6	15
A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2	14
Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt	14
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase	13
Purification and characterization of soluble recombinant Crimean-Congo hemorrhagic fever virus glycoprotein Gc expressed in mammalian 293F cells	13
Soft sensor modeling method for Pichia pastoris fermentation process based on substructure domain transfer learning	12
A novel allogeneic acellular matrix scaffold for porcine cartilage regeneration	12
Molecular and agro-morphological characterization of new barley genotypes in arid environments	12
Anti-inflammatory potential of aspergillus unguis SP51-EGY: TLR4-dependent effects & chemical diversity via Q-TOF LC-HRMS	12
Isolation and identification of antifungal, antibacterial and nematocide agents from marine bacillus gottheilii MSB1	11
Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24	11
A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility	11
Validation and calibration of a novel GEM biosensor for specific detection of Cd2+, Zn2+, and Pb2+	10
Aripiprazole-loaded niosome/chitosan-gold nanoparticles for breast cancer chemo-photo therapy	10
A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum	10
A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana	10
Derivation of a novel antimicrobial peptide from the Red Sea Brine Pools modified to enhance its anticancer activity against U2OS cells	9
ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment	9
Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle	9
Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system	9
Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress	9
Ferula latisecta gels for synthesis of zinc/silver binary nanoparticles: antibacterial effects against gram-negative and gram-positive bacteria and physicochemical characteristics	9
Saccharification and co-fermentation of lignocellulosic biomass by a cockroach-gut bacterial symbiont and yeast cocktail for bioethanol production	9
Enhancing the biotransformation of progesterone to the anticancer compound testololactone by Penicillium chrysogenum Ras3009: kinetic modelling and efficiency maximization	9
Synthesis and evaluation of nanosized aluminum MOF encapsulating Umbelliferon: assessing antioxidant, anti-inflammatory, and wound healing potential in an earthworm model	8
Aspergillus terreus camptothecin-sodium alginate/titanium dioxide nanoparticles as a novel nanocomposite with enhanced compatibility and anticancer efficiency in vivo	8
Green and environmentally friendly synthesis of silver nanoparticles with antibacterial properties from some medicinal plants	8
Organosolv-derived lipids from hemicellulose and cellulose, and pre-extracted tannins as additives upon hydrothermal liquefaction (HTL) of spruce bark lignins to bio-oil	8
Antifungal efficacy of Citrusfusion mediated silver nanoparticles in Candida species	8
An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes	8
High-performance internal circulation anaerobic granular sludge reactor for cattle slaughterhouse wastewater treatment and simultaneous biogas production	8
A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing	8
Coupling fermentation of glutamic acid and γ-polyglutamic acid and preparation of poly(amino acid) superabsorbent polymers	8
Development of a CHO cell line for stable production of recombinant antibodies against human MMP9	8
Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene	8
Insights from molecular docking and molecular dynamics on the potential of vitexin as an antagonist candidate against lipopolysaccharide (LPS) for microglial activation in neuroinflammation	7
Dual release of daptomycin and BMP-2 from a composite of β-TCP ceramic and ADA gelatin	7
Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates	7
Efficient extraction and antioxidant activity of polyphenols from Antrodia cinnamomea	7
The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar	7
Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms	7
Effects of solid lipid nanocarrier containing methyl urolithin A by coating folate-bound chitosan and evaluation of its anti-cancer activity	7
Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach	7
Orange peel-mediated synthesis of silver nanoparticles with antioxidant and antitumor activities	7
Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization	7
Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients	7
Integration of ZnO nanorods with silver ions by a facile co-precipitation for antimicrobial, larvicidal, and ovicidal activity	6
Development of a chemiluminescence assay for tissue plasminogen activator inhibitor complex and its applicability to gastric cancer	6
Choosing an appropriate somatic embryogenesis medium of carrot (Daucus carota L.) by data mining technology	6

Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21	6
Influence of low-intensity artificial light on the fatty acid profile of the biotechnologically important culinary mushroom Pleurotus eryngii in vitro	6
The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa	6
Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences	6
rocF affects the production of tetramethylpyrazine in fermented soybeans with Bacillus subtilis BJ3-2	6
Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications	6
Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant	6
Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci	6
Research on the targeted improvement of the yield of a new VB12-producing strain, Ensifer adhaerens S305, based on genomic and transcriptomic analysis	6
Microarray-based evaluation of selected recombinant timothy grass allergens expressed in E. Coli and N. Benthamiana	6
Toxic effects of trace phenol/guanidine isothiocyanate (P/GI) on cells cultured nearby in covered 96-well plates	5
Early osteogenic differentiation of human dental stem cells by gelatin/calcium phosphate- Punica granatum nanocomposite scaffold	5
Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes	5
Efficient gene delivery into the embryonic chicken brain using neuron-specific promoters and in ovo electroporation	5
Effect of different decellularization protocols on reendothelialization with human cells for a perfused renal bioscaffold of the rat	5
In vitro evaluation of PLGA loaded hesperidin on colorectal cancer cell lines: an insight into nano delivery system	5
Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori	5
Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation	5
Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities	5
Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis	5
MiR-125b-5p ameliorates ox-LDL-induced vascular endothelial cell dysfunction by negatively regulating TNFSF4/TLR4/NF-κB signaling	5
Comparison of lipidome profiles in serum from lactating dairy cows supplemented with Acremonium terrestris culture based on UPLC-QTRAP-MS/MS	5
Determination of serum CA724 levels using fluorescence immunochromatography	5
Antibacterial and antibiofilm potentials of vancomycin-loaded niosomal drug delivery system against methicillin-resistant Staphylococcus aureus (MRSA) infections	5
Antifungal, toxicological, and colorimetric properties of Origanum vulgare, Moringa oleifera, and Cinnamomum verum essential oils mixture against Egyptian Prince Yusuf Palace deteriorative fungi	4
Adsorption of Hg2+/Cr6+ by metal-binding proteins heterologously expressed in Escherichia coli	4
Statistical optimization of pectinases from thermophilic Aspergillus fumigatus BT-4 employing response surface methodology through submerged fermentation using agricultural wastes	4
An online soft sensor method for biochemical reaction process based on JS-ISSA-XGBoost	4
Impacts of loading thymoquinone to gold or silver nanoparticles on the efficacy of anti-tumor treatments in breast cancer with or without chemotherapeutic cisplatin	4
Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability	4
Extract optimization and biological activities of Otidea onotica using Artificial Neural Network-Genetic Algorithm and response surface methodology techniques	4
Enhanced vitamin B12 production by isolated Bacillus strains with the application of response surface methodology	4
Amino terminal recognition by a CCR6 chemokine receptor antibody blocks CCL20 signaling and IL-17 expression via β-arrestin	4
Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function	4
Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa	4
Development of Virus-Like Particles (VLPs) for Hepatitis C Virus genotype 4: a novel approach for vaccine development in Egypt	4
UGT708S6 from Dendrobium catenatum, catalyzes the formation of flavonoid C-glycosides	3
Optimization, characterization and biosafety of carotenoids produced from whey using Micrococcus luteus	3
A solution for highly efficient electroporation of primary cytotoxic T lymphocytes	3
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity	3
Use of immunoinformatics and the simulation approach to identify Helicobacter pylori epitopes to design a multi-epitope subunit vaccine for B- and T-cells	3
Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids	3
Correction: Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides	3
Kinetics of cellulase-free endo xylanase hyper-synthesis by Aspergillus Niger using wheat bran as a potential solid substrate	3
A cleavable peptide adapter augments the activity of targeted toxins in combination with the glycosidic endosomal escape enhancer SO1861	3
Analysis of hsa_circ_0136256 as a biomarker for fibrosis in systemic sclerosis	3
Nitric oxide mediates positive regulation of Nostoc flagelliforme polysaccharide yield via potential S-nitrosylation of G6PDH and UGDH	3
Lipid extraction and analysis of microalgae strain pectinodesmus PHM3 for biodiesel production	3
Extract optimization of Ulva lactuca L. and biological activities of optimized extracts	2
Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides	2
Suppression of mycotoxins production and efficient chelation of heavy metals using natural melanin originated from Aspergillus flavus and Aspergillus carbonarius	2
Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis	2
A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers	2
In vitro assessment of the effect of magnetic fields on efficacy of biosynthesized selenium nanoparticles by Alborzia kermanshahica	2
Multiprotein collagen/keratin hydrogel promoted myogenesis and angiogenesis of injured skeletal muscles in a mouse model	2
Artificial Neural Network - Multi-Objective Genetic Algorithm based optimization for the enhanced pigment accumulation in Synechocystis sp. PCC 6803	2
Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer	2
Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages	2
Screening of Candida spp. in wastewater in Brazil during COVID-19 pandemic: workflow for monitoring fungal pathogens	2
Potential protective efficacy of biogenic silver nanoparticles synthesised from earthworm extract in a septic mice model	2
Limonene encapsulated alginate/collagen as antibiofilm drug against Acinetobacter baumannii	2
Modification of improved-genome editing via oviductal nucleic acids delivery (i-GONAD)-mediated knock-in in rats	2
Antifungal activity and biocompatibility assessment with molecular docking and dynamic simulations of new pyrazole derivatives	2
Self-limiting fall armyworm: a new approach in development for sustainable crop protection and resistance management	2
Metabolic studies of Ogataea polymorpha using nine different corn steep liquors	2
Production of rhamnolipid biosurfactants in solid-state fermentation: process optimization and characterization studies	2
Chemical and Heat Treatment for Viral Inactivation in Porcine-Derived Gelatin	2
A study on the processing technology for Rhizoma Coptidis	2
Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species	2
αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8	2
Solubility of lamotrigine in age-specific biorelevant media that simulated the fasted- and fed-conditions of the gastric and intestinal environments in pediatrics and adults: implications for traditio	2
Development of a chemiluminescent immunoassay based on magnetic solid phase for quantification of homocysteine in human serum	2
An effective device to enable consistent scratches for in vitro scratch assays	2
Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum	2