OOIR: Observatory of International Research

Papers

(The median citation count of BMC Biotechnology is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-11-01 to 2024-11-01.)

Article	Citations
Exosomes for mRNA delivery: a novel biotherapeutic strategy with hurdles and hope	66
Optimization and scale-up of α-amylase production by Aspergillus oryzae using solid-state fermentation of edible oil cakes	59
The osteogenic differentiation of human dental pulp stem cells in alginate-gelatin/Nano-hydroxyapatite microcapsules	50
Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa	41
Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity	34
Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures	31
Repurposing novel therapeutic candidate drugs for coronavirus disease-19 based on protein-protein interaction network analysis	30
Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro	29
An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes	28
Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production	24
The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar	23
A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)	22
A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana	22
Lyso-phosphatidylethanolamine primes the plant immune system and promotes basal resistance against hemibiotrophic pathogens	21
Bacillus sp. CSK2 produced thermostable alkaline keratinase using agro-wastes: keratinolytic enzyme characterization	21
CRISPR/Cas9-mediated knockout of clinically relevant alloantigenes in human primary T cells	21
Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics	20
Production of surfactant-stable keratinase from Bacillus cereus YQ15 and its application as detergent additive	19
Levan from Leuconostoc citreum BD1707: production optimization and changes in molecular weight distribution during cultivation	19
Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability	19
Design of a new lyoprotectant increasing freeze-dried Lactobacillus strain survival to long-term storage	19
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity	18
Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis	17
Tenebrio molitor: possible source of polystyrene-degrading bacteria	17
Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids	16

Insights from molecular docking and molecular dynamics on the potential of vitexin as an antagonist candidate against lipopolysaccharide (LPS) for microglial activation in neuroinflammation	16
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)	15
Self-limiting fall armyworm: a new approach in development for sustainable crop protection and resistance management	14
Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate	14
Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium	14
Evaluating the biocontrol potential of Canadian strain Bacillus velezensis 1B-23 via its surfactin production at various pHs and temperatures	14
The mechanism of degradation of alizarin red by a white-rot fungus Trametes gibbosa	14
Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri	13
Optimization of lipase production using fungal isolates from oily residues	12
Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency	11
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase	11
Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures	11
Target identification of small molecules: an overview of the current applications in drug discovery	11
High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate	11
Efficient extraction and antioxidant activity of polyphenols from Antrodia cinnamomea	11
Mining of two novel aldehyde dehydrogenases (DHY-SC-VUT5 and DHY-G-VUT7) from metagenome of hydrocarbon contaminated soils	11
Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)	11
Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)	11
Recombinant laccase rPOXA 1B real-time, accelerated and molecular dynamics stability study	10
Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize	10
Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277	10
Antifungal screening of selenium nanoparticles biosynthesized by microcystin-producing Desmonostoc alborizicum	10
Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating	10
Generation of genome-edited dogs by somatic cell nuclear transfer	10
Effectiveness of recombinant Escherichia coli on the production of (R)-(+)-perillyl alcohol	9
Development of a human phage display-derived anti-PD-1 scFv antibody: an attractive tool for immune checkpoint therapy	9
Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide	9
Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification	9
Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis	9
Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design	9
Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids	9
Green and environmentally friendly synthesis of silver nanoparticles with antibacterial properties from some medicinal plants	9
Genome-scale model reconstruction of the methylotrophic yeast Ogataea polymorpha	8
Transient light-activated gene expression in Chinese hamster ovary cells	8
Modification of improved-genome editing via oviductal nucleic acids delivery (i-GONAD)-mediated knock-in in rats	8
Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass	8
Production of rhamnolipid biosurfactants in solid-state fermentation: process optimization and characterization studies	8
Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA	8
Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle	8
Optimizations for identifying reference genes in bone and cartilage bioengineering	8
Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses	8
In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract	7
Development of a microbial protease for composting swine carcasses, optimization of its production and elucidation of its catalytic hydrolysis mechanism	7
Computational modeling of PET tracer distribution in solid tumors integrating microvasculature	7
Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda)	7
Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system	7
Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms	7
Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid	6
Chloroplast genome sequencing based on genome skimming for identification of Eriobotryae Folium	6
Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia	6

Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation	6
Automatic identification of benign pigmented skin lesions from clinical images using deep convolutional neural network	6
Optimization of growth regulators on in vitro propagation of Moringa stenopetala from shoot explants	6
Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris	6
Establishment of a genetically engineered chicken DF-1 cell line for efficient amplification of influenza viruses in the absence of trypsin	6
Characterization of a highly xylose tolerant β-xylosidase isolated from high temperature horse manure compost	6
Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function	6
Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol	5
Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins	5
Integration of ZnO nanorods with silver ions by a facile co-precipitation for antimicrobial, larvicidal, and ovicidal activity	5
Amino terminal recognition by a CCR6 chemokine receptor antibody blocks CCL20 signaling and IL-17 expression via β-arrestin	5
A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers	5
Development of a CHO cell line for stable production of recombinant antibodies against human MMP9	5
Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds	5
Improving the catalytic characteristics of phenolic acid decarboxylase from Bacillus amyloliquefaciens by the engineering of N-terminus and C-terminus	5
Engineered FGF19ΔKLB protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model	5
Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences	5
Aspergillus terreus camptothecin-sodium alginate/titanium dioxide nanoparticles as a novel nanocomposite with enhanced compatibility and anticancer efficiency in vivo	5
Production of IgY polyclonal antibody against diphtheria toxin and evaluation of its neutralization effect by Vero cell assay	5
Creaming behavior prediction of argan oil in water emulsion stabilized by lacto-fermentation: creaming index	5
Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa	4
A fully human connective tissue growth factor blocking monoclonal antibody ameliorates experimental rheumatoid arthritis through inhibiting angiogenesis	4
Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes	4
Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus	4
Comparison of the effects of nitrogen-, sulfur- and combined nitrogen- and sulfur-deprivations on cell growth, lipid bodies and gene expressions in Chlamydomonas reinhardtii cc5373-sta6	4
Study on corrosion behavior of X80 steel under stripping coating by sulfate reducing bacteria	4
Identification and characterization of a new soybean promoter induced by Phakopsora pachyrhizi, the causal agent of Asian soybean rust	4
Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model	4
Development of an itaconic acid production process with Ustilaginaceae on alternative feedstocks	4
Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori	4
Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential proteomic analysis	4
rocF affects the production of tetramethylpyrazine in fermented soybeans with Bacillus subtilis BJ3-2	4
A computational approach to optimising laccase-mediated polyethylene oxidation through carbohydrate-binding module fusion	4
Development of an efficient veterinary rabies vaccine production process in the avian suspension cell line AGE1.CR.pIX	4
Preparation, purification, and biochemical of fat-degrading bacterial enzymes from pig carcass compost and its application	4
αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8	4
An effective device to enable consistent scratches for in vitro scratch assays	4
Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter	4
Effect of poly (lactic-co-glycolic acid) polymer nanoparticles loaded with vancomycin against Staphylococcus aureus biofilm	4
Preparation and characterization of propolis reinforced eggshell membrane/ GelMA composite hydrogel for biomedical applications	3
Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis, infesting hot peppers	3
A study on the processing technology for Rhizoma Coptidis	3
Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach	3
Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci	3
The impact of applying various de novo assembly and correction tools on the identification of genome characterization, drug resistance, and virulence factors of clinical isolates using ONT sequencing	3
Highly multiplexed targeted sequencing strategy for infectious disease surveillance	3
A nano-Liposomal formulation potentiates antioxidant, anti-inflammatory, and fibrinolytic activities of Allolobophora caliginosa coelomic fluid: formulation and characterization	3
The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa	3
Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology	3
In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization	3
Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion	3
Coupling fermentation of glutamic acid and γ-polyglutamic acid and preparation of poly(amino acid) superabsorbent polymers	2
Determination of serum CA724 levels using fluorescence immunochromatography	2
Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter pla	2
Whole-cell catalyze L-dopa to dopamine via co-expression of transport protein AroP in Escherichia coli	2
Preparation of an anti-NEK2 monoclonal antibody and its application in liver cancer	2
A solution for highly efficient electroporation of primary cytotoxic T lymphocytes	2
Use of immunoinformatics and the simulation approach to identify Helicobacter pylori epitopes to design a multi-epitope subunit vaccine for B- and T-cells	2
Biological modification of pentosans in wheat B starch wastewater and preparation of a composite film	2
The feasibility of sodium hydroxide pretreatment of rice straw for solid substrate preparation to enhance laccase production by solid state fermentation	2
Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene	2
Characterization, modeling, and anticancer activity of L.arginase production from marine Bacillus licheniformis OF2	2
Polyphyllin B inhibited STAT3/NCOA4 pathway and restored gut microbiota to ameliorate lung tissue injury in cigarette smoke-induced mice	2
Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21	2
Simplified plasmid cloning with a universal MCS design and bacterial in vivo assembly	2
Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody	2
Fabrication and characterization of metformin-loaded PLGA/Collagen nanofibers for modulation of macrophage polarization for tissue engineering and regenerative medicine	2
In vitro assessment of the effect of magnetic fields on efficacy of biosynthesized selenium nanoparticles by Alborzia kermanshahica	2
Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation	2
Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts	2
Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics	2
Oil–water partition coefficient preparation and detection in the dihydroartemisinin self-emulsifying drug delivery system	2
A novel allogeneic acellular matrix scaffold for porcine cartilage regeneration	2
Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt	2
Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein	2