OOIR: Observatory of International Research

Papers

(The median citation count of BMC Biotechnology is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-03-01 to 2024-03-01.)

Article	Citations
Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1	57
Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR	54
Exosomes for mRNA delivery: a novel biotherapeutic strategy with hurdles and hope	52
Optimization and scale-up of α-amylase production by Aspergillus oryzae using solid-state fermentation of edible oil cakes	47
Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation	43
The osteogenic differentiation of human dental pulp stem cells in alginate-gelatin/Nano-hydroxyapatite microcapsules	40
A novel high performance in-silico screened metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions	35
Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system	35
Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa	30
Repurposing novel therapeutic candidate drugs for coronavirus disease-19 based on protein-protein interaction network analysis	29
Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes	26
Phytoremediation effect of Medicago sativa colonized by Piriformospora indica in the phenanthrene and cadmium co-contaminated soil	24
GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system	23
An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes	22
Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro	22
Bacillus sp. CSK2 produced thermostable alkaline keratinase using agro-wastes: keratinolytic enzyme characterization	21
Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures	21
The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar	20
Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil	20
Lyso-phosphatidylethanolamine primes the plant immune system and promotes basal resistance against hemibiotrophic pathogens	19
Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications	19
Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity	19
Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production	19
A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)	18
Process optimization and effect of thermal, alkaline, H2O2 oxidation and combination pretreatment of sewage sludge on solubilization and anaerobic digestion	18

Levan from Leuconostoc citreum BD1707: production optimization and changes in molecular weight distribution during cultivation	17
Attenuation of intestinal inflammation in IL-10 deficient mice by a plasmid carrying Lactococcus lactis strain	17
Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability	16
CRISPR/Cas9-mediated knockout of clinically relevant alloantigenes in human primary T cells	16
Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation	16
A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana	16
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity	15
Tofu processing wastewater as a low-cost substrate for high activity nattokinase production using Bacillus subtilis	15
Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids	14
Design of a new lyoprotectant increasing freeze-dried Lactobacillus strain survival to long-term storage	14
Insights from molecular docking and molecular dynamics on the potential of vitexin as an antagonist candidate against lipopolysaccharide (LPS) for microglial activation in neuroinflammation	13
Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents	12
Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate	12
Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium	12
Evaluating the biocontrol potential of Canadian strain Bacillus velezensis 1B-23 via its surfactin production at various pHs and temperatures	11
Kojic acid repurposing as a pancreatic lipase inhibitor and the optimization of its production from a local Aspergillus oryzae soil isolate	11
Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics	11
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)	11
An improved quantitative real-time polymerase chain reaction technology for Helicobacter pylori detection in stomach tissue and its application value in clinical precision testing	11
Production of surfactant-stable keratinase from Bacillus cereus YQ15 and its application as detergent additive	11
Optimization of lipase production using fungal isolates from oily residues	11
An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea	11
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase	11
Mining of two novel aldehyde dehydrogenases (DHY-SC-VUT5 and DHY-G-VUT7) from metagenome of hydrocarbon contaminated soils	10
Gelatin nanoparticles enable water dispersibility and potentialize the antimicrobial activity of Buriti (Mauritia flexuosa) oil	10
Recombinant laccase rPOXA 1B real-time, accelerated and molecular dynamics stability study	10
High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate	10
Optimised production of an anti-fungal antibody in Solanaceae hairy roots to develop new formulations against Candida albicans	9
Development and biological activity of long-acting recombinant human interferon-α2b	9
Self-limiting fall armyworm: a new approach in development for sustainable crop protection and resistance management	9
Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277	9
Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures	9
Screening of miRNAs in plasma as a diagnostic biomarker for cardiac disease based on optimization of extraction and qRT-PCR condition assay through amplification efficiency	9
Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)	9
Synergistic interaction of hTGF-β3 with hBMP-6 promotes articular cartilage formation in chitosan scaffolds with hADSCs: implications for regenerative medicine	9
Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids	8
The mechanism of degradation of alizarin red by a white-rot fungus Trametes gibbosa	8
Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide	8
One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies	8
Tenebrio molitor: possible source of polystyrene-degrading bacteria	8
Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri	8
Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating	8
Development of a human phage display-derived anti-PD-1 scFv antibody: an attractive tool for immune checkpoint therapy	8
Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome	8
Generation of genome-edited dogs by somatic cell nuclear transfer	7
Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize	7
Optimizations for identifying reference genes in bone and cartilage bioengineering	7
Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system	7
Highly enhanced ELISA sensitivity using acetylated chitosan surfaces	7
Interference chromatography: a novel approach to optimizing chromatographic selectivity and separation performance for virus purification	7

Transient light-activated gene expression in Chinese hamster ovary cells	7
Creation of Golden Gate constructs for gene doctoring	7
Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination	7
Modification of improved-genome editing via oviductal nucleic acids delivery (i-GONAD)-mediated knock-in in rats	7
Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system	7
Preliminary study of the production of metabolites from in vitro cultures of C. ensiformis	7
Recombinant PAL/PilE/FlaA DNA vaccine provides protective immunity against Legionella pneumophila in BALB/c mice	7
Effectiveness of recombinant Escherichia coli on the production of (R)-(+)-perillyl alcohol	6
Characterization of a highly xylose tolerant β-xylosidase isolated from high temperature horse manure compost	6
Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle	6
Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function	6
Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass	6
Utilization of egg-laying hens (Gallus Gallus domesticus) for production of ingredients for human consumption and animal feed	6
In vitro biosynthesis of Ag, Au and Te-containing nanostructures by Exiguobacterium cell-free extracts	6
Genome-scale model reconstruction of the methylotrophic yeast Ogataea polymorpha	6
Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design	5
Evaluation of Hydra HALT-1 as a toxin moiety for recombinant immunotoxin	5
Efficient extraction and antioxidant activity of polyphenols from Antrodia cinnamomea	5
Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia	5
Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)	5
Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid	5
Chloroplast genome sequencing based on genome skimming for identification of Eriobotryae Folium	5
Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA	5
Improving the catalytic characteristics of phenolic acid decarboxylase from Bacillus amyloliquefaciens by the engineering of N-terminus and C-terminus	5
Antifungal screening of selenium nanoparticles biosynthesized by microcystin-producing Desmonostoc alborizicum	5
Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli	5
Establishment of a genetically engineered chicken DF-1 cell line for efficient amplification of influenza viruses in the absence of trypsin	5
Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification	5
Amino terminal recognition by a CCR6 chemokine receptor antibody blocks CCL20 signaling and IL-17 expression via β-arrestin	5
Production of IgY polyclonal antibody against diphtheria toxin and evaluation of its neutralization effect by Vero cell assay	5
Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential proteomic analysis	4
Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms	4
In vitro propagation of three mosaic disease resistant cassava cultivars	4
Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology	4
Development of a CHO cell line for stable production of recombinant antibodies against human MMP9	4
Computational modeling of PET tracer distribution in solid tumors integrating microvasculature	4
Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model	4
Creaming behavior prediction of argan oil in water emulsion stabilized by lacto-fermentation: creaming index	4
Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes	4
Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays	4
Study on corrosion behavior of X80 steel under stripping coating by sulfate reducing bacteria	4
Lentivirus expressing shRNAs inhibit the replication of contagious ecthyma virus by targeting DNA polymerase gene	4
Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris	4
Aspergillus terreus camptothecin-sodium alginate/titanium dioxide nanoparticles as a novel nanocomposite with enhanced compatibility and anticancer efficiency in vivo	4