BMC Biotechnology

Article	Citations
Optimization of chemical transfection in airway epithelial cell lines	66
MiR-125b-5p ameliorates ox-LDL-induced vascular endothelial cell dysfunction by negatively regulating TNFSF4/TLR4/NF-κB signaling	61
Early osteogenic differentiation of human dental stem cells by gelatin/calcium phosphate- Punica granatum nanocomposite scaffold	43
HPV16 mutant E6/E7 construct is protective in mouse model	34
Enhancing the biotransformation of progesterone to the anticancer compound testololactone by Penicillium chrysogenum Ras3009: kinetic modelling and efficiency maximization	30
In vitro evaluation of PLGA loaded hesperidin on colorectal cancer cell lines: an insight into nano delivery system	28
High-performance internal circulation anaerobic granular sludge reactor for cattle slaughterhouse wastewater treatment and simultaneous biogas production	26
Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities	25
Toxic effects of trace phenol/guanidine isothiocyanate (P/GI) on cells cultured nearby in covered 96-well plates	25
Validation of a rapid collagenase activity detection technique based on fluorescent quenched gelatin with synovial fluid samples	22
Development of a CHO cell line for stable production of recombinant antibodies against human MMP9	21
Aspergillus terreus camptothecin-sodium alginate/titanium dioxide nanoparticles as a novel nanocomposite with enhanced compatibility and anticancer efficiency in vivo	21
Development of a chemiluminescent immunoassay based on magnetic solid phase for quantification of homocysteine in human serum	19
Establishment of a novel cell line for producing replication-competent adenovirus-free adenoviruses	19
Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass	19
Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair	18
An efficient and specific CRISPR-Cas9 genome editing system targeting soybean phytoene desaturase genes	17
Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata	17
Extraction and analysis of high-quality chloroplast DNA with reduced nuclear DNA for medicinal plants	16
Expression and characterization of recombinant IL-1Ra in Aspergillus oryzae as a system	16
Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori	15
Screening of Candida spp. in wastewater in Brazil during COVID-19 pandemic: workflow for monitoring fungal pathogens	14
Simplified cloning and isolation of peptides from “sandwiched” SUMO-peptide-intein fusion proteins	14
Development of a thrombin-antithrombin complex detection kit and study in venous thromboembolism complicated by cervical cancer	13
Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation	12

Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology	11
Antimicrobial and cytotoxic activities of flavonoid and phenolics extracted from Sepia pharaonis ink (Mollusca: Cephalopoda)	11
Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle	11
A nano-Liposomal formulation potentiates antioxidant, anti-inflammatory, and fibrinolytic activities of Allolobophora caliginosa coelomic fluid: formulation and characterization	11
Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide	11
Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion	11
Genome-scale model reconstruction of the methylotrophic yeast Ogataea polymorpha	11
Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts	11
Fabrication of apigenin and adenosine-loaded nanoparticles against doxorubicin-induced myocardial infarction by reducing inflammation and oxidative stress	10
Screening approaches and potential of isolated lactic acid bacteria for improving fermentation of Saccharina latissima	10
Comparison of lipidome profiles in serum from lactating dairy cows supplemented with Acremonium terrestris culture based on UPLC-QTRAP-MS/MS	10
Fertility protective effects of Brillantaisia patula leaf extract against cyclophosphamide-induced ovarian damage in Wistar rats	10
Levan from Leuconostoc citreum BD1707: production optimization and changes in molecular weight distribution during cultivation	10
Characterization, modeling, and anticancer activity of L.arginase production from marine Bacillus licheniformis OF2	10
Evaluating the impact of Cold plasma on Seedling Growth properties, seed germination, and soybean antioxidant enzyme activity	10
Engineered FGF19ΔKLB protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model	10
Green and environmentally friendly synthesis of silver nanoparticles with antibacterial properties from some medicinal plants	10
A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing	9
Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides	9
Synergistic effect of zinc oxide-cinnamic acid nanoparticles for wound healing management: in vitro and zebrafish model studies	9
Chemical and Heat Treatment for Viral Inactivation in Porcine-Derived Gelatin	9
Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter pla	9
A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers	8
Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene	8
Insights from molecular docking and molecular dynamics on the potential of vitexin as an antagonist candidate against lipopolysaccharide (LPS) for microglial activation in neuroinflammation	8
Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes	8
Modification of improved-genome editing via oviductal nucleic acids delivery (i-GONAD)-mediated knock-in in rats	8
Development of a microbial protease for composting swine carcasses, optimization of its production and elucidation of its catalytic hydrolysis mechanism	8
Improving the regeneration rate of deep lethal mutant protoplasts by fusion to promote efficient L-lysine fermentation	8
Efficient gene delivery into the embryonic chicken brain using neuron-specific promoters and in ovo electroporation	7
Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function	7
Determination of serum CA724 levels using fluorescence immunochromatography	7
Development of Virus-Like Particles (VLPs) for Hepatitis C Virus genotype 4: a novel approach for vaccine development in Egypt	7
Repurposing novel therapeutic candidate drugs for coronavirus disease-19 based on protein-protein interaction network analysis	7
αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8	7
Biocompatibility and potential anticancer activity of gadolinium oxide (Gd2O3) nanoparticles against nasal squamous cell carcinoma	7
In vitro assessment of the effect of magnetic fields on efficacy of biosynthesized selenium nanoparticles by Alborzia kermanshahica	6
Generation of genome-edited dogs by somatic cell nuclear transfer	6
Reductive enzymatic dynamic kinetic resolution affording 115 g/L (S)-2-phenylpropanol	6
Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize	6
Solubility of lamotrigine in age-specific biorelevant media that simulated the fasted- and fed-conditions of the gastric and intestinal environments in pediatrics and adults: implications for traditio	6
Evaluating the biocontrol potential of Canadian strain Bacillus velezensis 1B-23 via its surfactin production at various pHs and temperatures	6
3D printing of Ceffe-infused scaffolds for tailored nipple-like cartilage development	5
The feasibility of sodium hydroxide pretreatment of rice straw for solid substrate preparation to enhance laccase production by solid state fermentation	5
Correction to: Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses	5
Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach	5
Systematic design and evaluation of aptamers for VEGF and PlGF biomarkers of Preeclampsia	5
Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)	5
The mechanism of degradation of alizarin red by a white-rot fungus Trametes gibbosa	5
Coupling fermentation of glutamic acid and γ-polyglutamic acid and preparation of poly(amino acid) superabsorbent polymers	5

Synthesis and evaluation of nanosized aluminum MOF encapsulating Umbelliferon: assessing antioxidant, anti-inflammatory, and wound healing potential in an earthworm model	5
Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris	5
An improved HPAEC-PAD method for the determination of D-glucuronic acid and 4-O-methyl-D-glucuronic acid from polymeric and oligomeric xylan	5
Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis	5
Antibacterial and antibiofilm potentials of vancomycin-loaded niosomal drug delivery system against methicillin-resistant Staphylococcus aureus (MRSA) infections	4
Effect of different decellularization protocols on reendothelialization with human cells for a perfused renal bioscaffold of the rat	4
Improving the catalytic characteristics of phenolic acid decarboxylase from Bacillus amyloliquefaciens by the engineering of N-terminus and C-terminus	4
Optimization of lipase production using fungal isolates from oily residues	4
Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species	4
Potential protective efficacy of biogenic silver nanoparticles synthesised from earthworm extract in a septic mice model	4
Biosafety and pharmacokinetic characteristics of polyethylene pyrrolidone modified nano selenium in rats	4
Genome-scale insights into the metabolic versatility of Limosilactobacillus reuteri	4
Creaming behavior prediction of argan oil in water emulsion stabilized by lacto-fermentation: creaming index	4
Fusarium verticillioides pigment: production, response surface optimization, gamma irradiation and encapsulation studies	4
Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia	4
A model approach to show that monocytes can enter microporous β-TCP ceramics	4
Organosolv-derived lipids from hemicellulose and cellulose, and pre-extracted tannins as additives upon hydrothermal liquefaction (HTL) of spruce bark lignins to bio-oil	4
The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar	4
Characterization of a highly xylose tolerant β-xylosidase isolated from high temperature horse manure compost	4
An online soft sensor method for biochemical reaction process based on JS-ISSA-XGBoost	4
Limonene encapsulated alginate/collagen as antibiofilm drug against Acinetobacter baumannii	4
Efficient extraction and antioxidant activity of polyphenols from Antrodia cinnamomea	4
In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization	3
Biological modification of pentosans in wheat B starch wastewater and preparation of a composite film	3
Development of a human phage display-derived anti-PD-1 scFv antibody: an attractive tool for immune checkpoint therapy	3
Design of a novel multi-epitope vaccine against Marburg virus using immunoinformatics studies	3
Biological activities of Hypericum spectabile extract optimized using artificial neural network combined with genetic algorithm application	3
Highly multiplexed targeted sequencing strategy for infectious disease surveillance	3
Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity	3
Lentiviral CRISPR-guided RNA library screening identified Adam17 as an upstream negative regulator of Procr in mammary epithelium	3
Development of an itaconic acid production process with Ustilaginaceae on alternative feedstocks	3
Rice yellow mottle virus is a suitable amplicon vector for an efficient production of an anti-leishmianiasis vaccine in Nicotiana benthamiana leaves	3
Minimizing IP issues associated with gene constructs encoding the Bt toxin - a case study	3
Fabrication and characterization of metformin-loaded PLGA/Collagen nanofibers for modulation of macrophage polarization for tissue engineering and regenerative medicine	3
Antifungal screening of selenium nanoparticles biosynthesized by microcystin-producing Desmonostoc alborizicum	3
Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein	3
A study on the processing technology for Rhizoma Coptidis	3
Amino terminal recognition by a CCR6 chemokine receptor antibody blocks CCL20 signaling and IL-17 expression via β-arrestin	3
Adsorption of Hg2+/Cr6+ by metal-binding proteins heterologously expressed in Escherichia coli	2
Iron/Copper/Phosphate nanocomposite as antimicrobial, antisnail, and wheat growth-promoting agent	2
Design of a new lyoprotectant increasing freeze-dried Lactobacillus strain survival to long-term storage	2
A fully human connective tissue growth factor blocking monoclonal antibody ameliorates experimental rheumatoid arthritis through inhibiting angiogenesis	2
Suppression of mycotoxins production and efficient chelation of heavy metals using natural melanin originated from Aspergillus flavus and Aspergillus carbonarius	2
940 nm diode laser induced differentiation of human adipose derived stem cells to temporomandibular joint disc cells	2
A cleavable peptide adapter augments the activity of targeted toxins in combination with the glycosidic endosomal escape enhancer SO1861	2
Preparation, purification, and biochemical of fat-degrading bacterial enzymes from pig carcass compost and its application	2
Optimization and scale-up of α-amylase production by Aspergillus oryzae using solid-state fermentation of edible oil cakes	2
Antibacterial effect of cerium oxide nanoparticle against Pseudomonas aeruginosa	2
Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability	2
Orange peel-mediated synthesis of silver nanoparticles with antioxidant and antitumor activities	2
Antifungal, toxicological, and colorimetric properties of Origanum vulgare, Moringa oleifera, and Cinnamomum verum essential oils mixture against Egyptian Prince Yusuf Palace deteriorative fungi	2
Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen	2
Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates	2
Production of surfactant-stable keratinase from Bacillus cereus YQ15 and its application as detergent additive	2
Purification and characterization of soluble recombinant Crimean-Congo hemorrhagic fever virus glycoprotein Gc expressed in mammalian 293F cells	2
Transgenic Arabidopsis thaliana plants expressing bacterial γ-hexachlorocyclohexane dehydrochlorinase LinA	2
Enhanced vitamin B12 production by isolated Bacillus strains with the application of response surface methodology	2
Method for quantification of porcine type I interferon activity using luminescence, by direct and indirect means	2
Production of IgY polyclonal antibody against diphtheria toxin and evaluation of its neutralization effect by Vero cell assay	2
Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant	2
Statistical optimization of pectinases from thermophilic Aspergillus fumigatus BT-4 employing response surface methodology through submerged fermentation using agricultural wastes	2
Recombinant human enamelin produced in Escherichia coli promotes mineralization in vitro	2
Effect of poly (lactic-co-glycolic acid) polymer nanoparticles loaded with vancomycin against Staphylococcus aureus biofilm	2
Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA	1
Recombinant laccase rPOXA 1B real-time, accelerated and molecular dynamics stability study	1
Antioxidant, neuroprotective, and neuroblastoma cells (SH-SY5Y) differentiation effects of melanins and arginine-modified melanins from Daedaleopsis tricolor and Fomes fomentarius	1
Staphopain mediated virulence and antibiotic resistance alteration in co-infection of Staphylococcus aureus and Pseudomonas aeruginosa: an animal model	1
The impact of applying various de novo assembly and correction tools on the identification of genome characterization, drug resistance, and virulence factors of clinical isolates using ONT sequencing	1
Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology	1
Evaluation of RNA isolation methods for microRNA quantification in a range of clinical biofluids	1
Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids	1
Expanding the genetic toolbox for Cutaneotrichosporon oleaginosus employing newly identified promoters and a novel antibiotic resistance marker	1
Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms	1
Optimisation of indole acetic acid production by Neopestalotiopsis aotearoa endophyte isolated from Thymus vulgaris and its impact on seed germination of Ocimum basilicum	1
Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa	1
High level expression and biochemical characterization of an alkaline serine protease from Geobacillus stearothermophilus to prepare antihypertensive whey protein hydrolysate	1
Enhanced bone regeneration by low-intensity pulsed ultrasound and lipid microbubbles on PLGA/TCP 3D-printed scaffolds	1
Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model	1
Nutritional composition, lipid profile and stability, antioxidant activities and sensory evaluation of pasta enriched by linseed flour and linseed oil	1
One-step fermentation for producing xylo-oligosaccharides from wheat bran by recombinant Escherichia coli containing an alkaline xylanase	1

Integration of ZnO nanorods with silver ions by a facile co-precipitation for antimicrobial, larvicidal, and ovicidal activity	1
Microvesicles-delivering Smad7 have advantages over microvesicles in suppressing fibroblast differentiation in a model of Peyronie’s disease	1
A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2	1
Analysis of hsa_circ_0136256 as a biomarker for fibrosis in systemic sclerosis	1
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity	1
A new method for screening acute/chronic lymphocytic leukemia: dual-label time-resolved fluorescence immunoassay	1
Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody	1
Dual release of daptomycin and BMP-2 from a composite of β-TCP ceramic and ADA gelatin	1
Use of immunoinformatics and the simulation approach to identify Helicobacter pylori epitopes to design a multi-epitope subunit vaccine for B- and T-cells	1
Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt	1
Greenhouse test of spraying dsRNA to control the western flower thrips, Frankliniella occidentalis, infesting hot peppers	1