Biotechnology and Bioprocess Engineering

Article	Citations
Development of a glucose enzyme fuel cell based on thin film electrode using biocatalysts	37
Inhibition of Cyclopropane Fatty Acid Synthesis in the Membrane of Halophilic Halomonas socia CKY01 by Kanamycin	33
Advanced Whole-cell Conversion for D-allulose Production Using an Engineered Corynebacterium glutamicum	31
Homotaurine exhibits contrasting effects of DRD1-mediated thermogenesis-related regulators in C2C12 myoblasts and 3T3−L1 white adipocytes	26
Improvement of menopausal symptoms by oat hull extracts in ovariectomized animal model	25
The latest techniques for inducing macrophage differentiation	24
Improved enzyme-linked immunosorbent assay using surface-adhesive antibody-oriented immobilizing biolinker: a proof-of-concept study	24
Elucidation of the Mechanism and Kinetics of Ultrasonic Extraction of Paclitaxel from Plant Cell Cultures of Taxus chinensis	24
Bioprocess Challenges in Purification of Therapeutic Protein Charge Variants	22
Convolution Neural Network Approaches for Cancer Cell Image Classification	20
MiR-1297 and MiR-26a-5p Inhibit Cell Progression of Keratinocytes in Cholesteatoma Depending on the Regulation of BMI1	20
A comparison of metabolic engineering strategies applied in Yarrowia lipolytica for β-carotene production	18
CircBRMS1L Participates in Lipopolysaccharide-induced Chondrocyte Injury via the TLR4/NF-κB Pathway through Serving as a miR-142-5p Decoy	17
Recent Progress in Microalgal Squalene Production and Its Cosmetic Application	16
Regulated in Development and DNA Damage Response 1 Protects Hepatocytes Against Palmitate-induced Lipotoxicity	16
Multi-bioinspired Sprayable Nanotherapeutics for Tumor-Specific Focal Cancer Therapy	15
Impact of the Synthetic Scaffold Strategy on the Metabolic Pathway Engineering	14
Effect of reduced atmospheric pressures on the morphology and astaxanthin biosynthesis of microalga Haematococcus lacustris	14
Novel Synthesis of Ca2+-Doped MgAl2O3-G-SiO2 Mesoporous Nanospheres toward Sensing Effects for Selective Electrochemical Performance of Azithromycin	14
Albumin: A Multi-talented Clinical and Pharmaceutical Player	13
C13-apocarotenoids biosynthesis with engineered microbes	13
The Enzymatic Process of Macroalgae for Conversion into High-tech Bioproducts	13
Mechanistic Insight into Phenolic Compounds Toxicity and State-of-the-art Strategies for Enhancing the Tolerance of Escherichia coli to Phenolic Compounds	13
Assessment of Damnacanthus major Siebold & Zucc callus for antioxidative and moisturizing capacities using an artificial skin alternative	12
Cyclic AMP Responsive Element Binding Protein 3-like 4/AarF Domain Containing Kinase 5 Axis Facilitates Proliferation, Migration and Invasion of Lung Adenocarcinoma Cells by Modulating the TGFβ Pathwa	12

L-Dihydroxyphenylalanine (L-Dopa) Induces Brown-like Phenotype in 3T3-L1 White Adipocytes via Activation of Dopaminergic and β3-adrenergic Receptors	12
Trans-anethole Induces Thermogenesis via Activating SERCA/SLN Axis in C2C12 Muscle Cells	12
Efficient production of 1,3-propanediol from glycerol by a newly isolated soil bacterium using fed-batch fermentation	12
3D microcapsules for human bone marrow-derived mesenchymal stem cell biomanufacturing in a vertical-wheel bioreactor	12
Erratum to: Optimization of Vacuum Frying Process for Sweet Potato Chip Manufacturing Using Response Surface Methodology and Artificial Neural Network Model	12
Improving the Thermostability of α-Glucosidase from Xanthomonas campestris through Proline Substitutions Guided by Semi-rational Design	12
A Review of Microbial Molecular Profiling during Biomass Valorization	11
DNA Microarray-based Detection of Bacteria in Samples Containing Antibiotics: Effect of Antibiotics on the Performance of Pathogen Detection Assays	11
Recent Advances in the Utilization of Brown Macroalgae as Feedstock for Microbial Biorefinery	11
Suppressive Activities of Fisetin on Particulate Matter-induced Oxidative Stress	11
Drug-eluting Microneedles Embedded with Nanoparticles for Anti-inflammatory Purposes	11
Current Updates on COVID-19 Vaccines and Therapeutics: As of June 2022	11
Production of Tagatose by Whole-cell Bioconversion from Fructose Using Corynebacterium glutamicum	11
Fabrication of folic acid–cysteamine-modified silver nanoparticles as promising contrast agent for computed tomography imaging	10
Automatic cancer nuclei segmentation on histological images: comparison study of deep learning methods	10
Nanostructure Materials: Efficient Strategies for Circulating Tumor Cells Capture, Release, and Detection	10
Metallic Vessel with Mesh Culture Surface Fabricated Using Three-dimensional Printing Engineers Tissue Culture Environment	10
Agarwood Pill Enhances Immune Function in Cyclophosphamide-induced Immunosuppressed Mice	10
Regulatory Role of SFN Gene in Hepatocellular Carcinoma and Its Mechanism	10
Structural Study on the Impact of S239D/I332E Mutations in the Binding of Fc and FcγRIIIa	10
An Aligned Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Scaffold Fixed with Fibronectin to Enhance the Attachment and Growth of Human Endothelial Progenitor Cells	10
Ceramide’s Role and Biosynthesis: A Brief Review	9
Plant Platforms for Efficient Heterologous Protein Production	9
Bioimprinted Aspergillus niger Lipase Combined with Adsorption Immobilization by Resin and Its Experiment in Catalytic Synthesis of Sucrose-6-acetate	9
Renal Protective Effects of Sparstolonin B in a Mouse Model of Sepsis	9
Improvement of Tol2 Transposon System Enabling Efficient Protein Production in CHO Cells	9
Sodium-potassium Adenosine Triphosphatase α2 Subunit (ATP1A2) Negatively Regulates UCP1-dependent and UCP1-independent Thermogenesis in 3T3-L1 Adipocytes	9
Zero-valent Iron Enhances Acetate and Butyrate Production from Carbon Monoxide by Fonticella tunisiensis HN43	9
Enhancing the Thermostability of Cellulase from Clostridium thermocellum via Salt Bridge Interactions	9
Sensitive detection of SARS-CoV2 spike antibodies by a paper-based polypyrrole/reduced graphene oxide sensor	9
Inhibiting angiogenesis and osteogenesis by targeted regulation of KLK4 via the exosomal MiR-422a derived from bone mesenchymal stem cells	9
Continuous cell recycling in methylotrophic yeast Pichia pastoris to enhance product yields: a case study with Yarrowia lipolytica lipase Lip2	9
Cell-free Biosynthesis of Peptidomimetics	9
Interaction Between Hepatocytes and Proximal Tubular Epithelial Cells in Hypoxia-induced Lipotoxicity	9
Erratum to: Impact of the Synthetic Scaffold Strategy on the Metabolic Pathway Engineering	8
Candida rugosa Lipase Immobilization on Fe3O4 Coated Carboxyl Functionalised Multiwalled Carbon Nanotubes for Production of Food Flavour Esters	8
Green synthesis of gold nanoparticles using Acorus calamus leaf extract and study on their anti-alzheimer potential	8
Publisher Erratum to: Effects of gltA and arcA Mutations on Biomass and 1,3-Propanediol Production in Klebsiella pneumoniae	8
Selective Extraction of Chlorophyll a/Photosystem Polypeptides from Spirulina maxima Using Aqueous Two Phase Extraction	8
A study on the relationship between MDR1 mutation and ex vivo drug sensitivities of canine lymphomas	8
The Reductive Amination of Carbonyl Compounds Using Native Amine Dehydrogenase from Laribacter hongkongensis	8
Development of Origanum vulgare Cell Suspension Culture to Produce Polyphenols and the Stimulation Effect of Salicylic Acid Elicitation and Phenylalanine Feeding	8
The Inhibitory Effect of Corni Fructus against Oxidative Stress-induced Cellular Damage in C2C12 Murine Myoblasts	8
Investigation of β-phenylethanol production by a wild-type Pichia kudriavzevii isolated from jiuqu of rice-flavor baijiu	8
Ultrasound- and Negative Pressure-assisted Fractional Precipitation of Paclitaxel from Taxus chinensis	8
Redox switch protein Hsp33 has a novel zinc-dependent DNA binding function under cold stress in Escherichia coli	8
Schisandrae Fructus Ameliorates Topical Particulate Matter 2.5-induced Keratoconjunctivitis Sicca That Are Accompanied by Retinal and Lipid Metabolism Disorders	7
Metabolic modeling of microorganisms involved in anaerobic digestion	7
Development of Orthogonal Aminoacyl tRNA Synthetase Mutant with Enhanced Incorporation Ability with Para-azido-L-phenylalanine	7
Anti-obesity Potential of Lactobacillus spp. Isolated from Infant Feces	7

Identification Process and Physiological Properties of Transporters of Carboxylic Acids in Escherichia coli	7
Regulatory Effect on Skin Differentiation by Mevastatin in Psoriasis Model Using TNF-α and IL-17 Induced HaCaT Cells	7
Hyaluronic Acid-based Biomimetic Hydrogels for Tissue Engineering and Medical Applications	7
Tumor Homing Peptides as Fusion Partners of Therapeutic Proteins for Efficient Delivery to Cancer Cells	7
Photothermal heating of cell-free reactions for on-site production of recombinant proteins	7
Osteostimulating Ability of β-tricalcium Phosphate/collagen Composite as a Practical Bone-grafting Substitute: In vitro and in vivo Comparison Study with Commercial One	7
Biotechnological Advances and Trends in Engineering Trichoderma reesei towards Cellulase Hyperproducer	7
Adsorption of Lithium on Cell Surface as Nanoparticles through Lithium Binding Peptide Display in Recombinant Escherichia coli	7
Efficient biodegradation of low-density polyethylene by Pseudomonas plecoglossicida SYp2123 was observed through FT-IR and FE-SEM analysis	7
Efficient Production of Phenyllactic Acid by Whole-cell Biocatalysis with Cofactor Regeneration System	7
Recycle of sugarcane molasses by Aurantiochytrium sp. for high-value docosahexaenoic acid	7
Anti-cancer Effect of Hyoscyamus muticus Extract via Its Activation of Fas/FasL-ASK1-p38 Pathway	6
Evaluation of Pretreatment and GABA Production Using Levilactobacillus brevis Fermentation of the Seaweed Saccharina japonica	6
Isolation and characterization of two halophilic bacteria producing polyhydroxybutyrate from high-salt environment	6
An ultrasound–negative pressure cavitation adsorption method for the recovery of paclitaxel from cell-free culture supernatants of Taxus chinensis	6
Alpha-tocopherol-incorporating liposomes as efficient protectors from skin cell damages by single infrared-A irradiation with cold stress	6
Methane Based Continuous Culture of Methylosinus trichosporium for Production of Poly-3-hydroxybutyrate Using Membrane Recycle System	6
Improvements in Conventional Modeling Practices for Effective Simulation and Understanding of Microalgal Growth in Photobioreactors: an Experimental Study	6
Tuning the Assembly of Bispecific Antibodies by Playing on Differential Polypeptide Chain Molar Ratios	6
Analysis and Prediction of Surface Condition of Artificial Skin Based on CNN and ConvLSTM	6
Metabolic Engineering of Bacillus megaterium for the Production of β-alanine	6
Introduction of an AU-rich Element into the 5’ UTR of mRNAs Enhances Protein Expression in Escherichia coli by S1 Protein and Hfq Protein	6
Cre/Lox-based RMCE for Site-specific Integration in CHO Cells	6
Engineering of the Substrate Pocket of α-ketoglutaric Semialdehyde Dehydrogenase for Improving the Activity toward 3-hydroxypropanal	6
Enhanced 2,3-butanediol recovery from fermentation broth: a study on isobutanol/sodium chloride aqueous two-phase systems	6
Development of Auxotrophic Agrobacterium tumefaciens AGL1 by Tn5 Transposon for Rice (Oryza sativa L.) Transformation	6
Effects of Acid-fermented Food Wastewater in Microwave-based Direct Lipid Extraction from Wet Microalgae	6
Co-utilization of Maltose and Sodium Acetate via Engineered Corynebacterium glutamicum for Improved Itaconic Acid Production	6
An Overview on Wound Dressings and Sutures Fabricated by Electrospinning	6
Single Cell Analysis of Microalgae and Associated Bacteria Flora by Using Flow Cytometry	6
Development, physico-chemical characterization, and in vivo stability of a novel aglycosylated monoclonal antibody targeting FAM19A5	6
In vitro and in silico study on glucosylation of caprylyl glycol	6
Effect of Epidermal Growth Factor on the Colony-formation Ability of Porcine Spermatogonial Germ Cells	6
Lessons from Clostridial Genetics: Toward Engineering Acetogenic Bacteria	6
High-Throughput Production of Microcapsules for Human Bone Marrow Derived Mesenchymal Stem Cell Biomanufacturing in a Vertical-Wheel Bioreactor	5
Strategies to Enhance the Biosynthesis of Monounsaturated Fatty Acids in Escherichia coli	5
Ginkgolide B Suppresses TPA-induced Metastatic Potential in MCF-7 Human Breast Cancer Cells by Inhibiting MAPK/AP-1 Signaling	5
Extraction of the outer membrane protein pertactin from Bordetella pertussis with urea for the production of acellular pertussis vaccine	5
CRISPR/Cas12a-mediated electrochemical biosensor for the sensitive detection of Vibrio parahaemolyticus	5
Theabrownin in Black Tea Suppresses UVB-induced Matrix Metalloproteinase-1 Expression in HaCaT Keratinocytes	5
Oligopeptides derived from rice protein hydrolysates: absorption, bioactivity, preparation, debittering, and application	5
Enhanced production of scopolamine and atropine alkaloids in in vitro cell suspension cultures of Atropa belladonna L. under 2-iP, 2,4-D, and ornithine	5
Curcumin Stimulates UCP1-independent Thermogenesis in 3T3-L1 White Adipocytes but Suppresses in C2C12 Muscle Cells	5
An Algicidal Agent against Harmful Algae Using Novel N1-benzyl-N3, N3-diethylpropane-1,3-diamine Derivatives	5
In silico screening and validation of different dehydrogenases to produce 2,3-butanediol in Bacillus subtilis	5
Antioxidant and Anticancer Effects of Epsilon-Poly-L-lysine Produced by Two Novel Strains of Paenibacillus polymyxa HS6 and Stenotrophomonas maltophilia YS8	5
Transcriptional Characterization of the Effects of Macrophages on Inflammation-Induced Reconstructed Human Skin Equivalents	5
An integrated in silico reverse vaccinology approach for multi-epitope vaccine designing against Shigellosis caused by Shigella flexneri serotype X	5
ATP1A2 negatively regulates myogenic differentiation and thermogenesis in C2C12 myoblasts	5
Advancing biofoundry development: strategies and challenges	5
Increasing Demeclocycline Production in Streptomyces aureofaciens by Manipulating the Expression of a Novel SARP Family Regulator and Its Genes	5
Event-specific loop-mediated isothermal amplification for living modified cotton MON88701, MON531, MON15985, MON88913, and COT102	5
Stability Enhancement of Target Enzymes via Tyrosinase-Mediated Site-Specific Polysaccharide Coating	5
Circ_0005280 Protects Human Lens Epithelial Cells against H2O2-induced Apoptosis and Oxidative Stress though miR-326/PLCD3 Axis	5
Colorimetric detection of milk spoilage at low temperatures: a novel PDA/ZnO@NC membrane for pH-based freshness monitoring	5
Hyaluronic Acid Stimulated Enterocytic Differentiation of Intestinal Stem Cells and Enhanced Enteroid Grafting on Scaffolds	5
Improving the Microbial Production of Amino Acids: From Conventional Approaches to Recent Trends	5
Engineering potyvirus-like particles to display multiple copies of tuberculosis antigens	5
Bioactive Peptides and Its Alternative Processes: A Review	5
Enhanced cytotoxicity of natural killer cells with Zn-alginate hydrogel microspheres	5
Intracellular cholesterol efflux effects of mannose-beta cyclodextrin conjugates on cholesterol-laden foam cells	5
Omics and CRISPR in CHO bioproduction: a state-of-the-art review	5
Development of a Spacer-optimized Quenchbody against Tumor Necrosis Factor Alpha	5
Hydroxyapatite Derived from Marine Resources and their Potential Biomedical Applications	5
Quantitative Methods for Metabolite Analysis in Metabolic Engineering	5
Production of chitosan from Aspergillus niger and quantitative evaluation of the process using adapted analytical tools	5
Investigating the Performance of Machine Learning Methods in Predicting Functional Properties of the Hydrogenase Variants	4
Memristor device based on bioengineered elastin-like polypeptide and its bionanohybrid	4
Production of the Minor Ginsenoside F2 from the PPD-mix-type Major Ginsenosides Using a Novel Recombinant Glycoside Hydrolase from Novosphingobium aromaticivorans	4
Evaluation of the Effects, Causes, and Risks of Gold Nanorods Promoting Cell Proliferation	4
Elucidation of the Role of Gas Bubbles in the Fractional Precipitation of Paclitaxel	4
One-step spheroid formation via double-emulsion drops generated by a glass capillary-based microfluidic device for circadian rhythm investigation	4
Physicochemical Characteristics and Biogas Production Potentials of Olive Flounder and Starry Flounder Wastes	4
Rapid and Efficient BAC Recombineering: Gain & Loss Screening System	4
Hydrolyzed oyster extracts suppress lipopolysaccharide-mediated inflammation and oxidative stress in RAW264.7 murine macrophages	4
Chrysin stimulates UCP1-independent thermogenesis in 3T3-L1 adipocytes and mouse model	4
Formulation of Glycyrrhizic Acid-based Nanocomplexes for Enhanced Anti-cancer and Anti-inflammatory Effects of Curcumin	4
1-Deoxynojirimycin-producing bacteria: production, optimization, biosynthesis, biological activities	4
Development of Tat-fused drug binding protein to improve anti-cancer effect of mammalian target of rapamycin inhibitors	4
Study on a Low-temperature Cellulose-degrading Strain: Fermentation Optimization, Straw Degradation, and the Effect of Fermentation Broth on Seed Growth	4

Optimization of Vacuum Frying Process for Sweet Potato Chip Manufacturing Using Response Surface Methodology and Artificial Neural Network Model	4
Inhibitory Activities of GDX-365 on HMGB1-mediated Septic Responses	4
Improved Extracellular Enzyme-mediated Production of 7,10-dihydroxy-8(E)-octadecenoic Acid by Pseudomonas aeruginosa	4
Nanoemulsion as an Effective Inhibitor of Biofilm-forming Bacterial Associated Drug Resistance: An Insight into COVID Based Nosocomial Infections	4
Immobilization of Candida rugosa Lipase on Magnetic Biosilica Particles: Hydrolysis and Transesterification Studies	4
Safe and efficient treatment of drug-resistant colorectal cancer cells using kaempferol-loaded exosomes	4
Synthesis and Structure-activity Relationship Study of 2,4-dioxothiazolidin-5-ylidene Derivatives for 15-hydroxyprostaglandin Dehydrogenase Inhibitory Activity, Prostaglandin E2 Release, and Wound Hea	4
Self-assembled Nanocomplex Using Cellulose Nanocrystal Based on Zinc/DNA Nanocluster for Gene Delivery	4
ROSA26 BAC-based system enables continuous high-yield protein production	4
MiR-466d Targeting MMP13 Promotes the Differentiation of Osteoblasts Exposed to a Static Magnetic Field	4
Near-infrared and Thermo-sensitive Liposomes Incorporating Thiolated-carboxymethyl Cellulose-capped Gold Nanoparticles and Poly(N-isopropylacrylamide)	4
Tn5 transposase: a key tool to decrypt random transposition	4
Recent advancements in flavonoid production through engineering microbial systems	4
Reconstruction of genome-scale metabolic models of non-conventional yeasts: current state, challenges, and perspectives	4
Study for the Bio-CNG Recovery of Methane Gas in the Anaerobic Co-digestion Using Malaysian POME (Palm Oil Mill Effluent)	4
miR-182-5p Inhibits NKAPL Expression and Promotes the Proliferation of Osteosarcoma	4
The Role of Andrographolide on Skin Inflammations and Modulation of Skin Barrier Functions in Human Keratinocyte	4
Improvement of Lipid and Terpenoid Yield in Thraustochytrids Using Chemical Regulators: A Review	4
Poly(ε-caprolactone), zinc oxide nanoparticles, and chitosan scaffold as skin substitutes: mechanical strength and cell viability analysis	4
An efficient method for isolation and expansion of hematopoietic stem cells derived from human umbilical cord blood cells	4
Immobilization study of a monomeric oleate hydratase from Rhodococcus erythropolis	4
Virus-like Particles as Antiviral Vaccine: Mechanism, Design, and Application	4
A Cell-free Protein Synthesis Method for the Detection of Heavy Metal Ions Using a Personal Glucose Meter	4
d-Allulose Production from d-fructose by Putative Dolichol Phosphate Mannose Synthase from Bacillus sp. with Potential d-allulose 3-epimrase Activity	4
Y-shaped oligonucleotides: a promising platform for enhanced therapy with siRNA and CpG Oligodeoxyribonucleotides	4
Pan-genome Analysis Reveals Comparative Genomic Features of Central Metabolic Pathways in Methylorubrum extorquens	3
Commercial-scale Economic Comparison of Different Batch Modes for Upstream and Downstream Processing of Monoclonal Antibody	3
Development of funnel-type fluidized bed reactor system using microcarriers for cultures of adherent cells	3
Evaluation of Lipid-polyethylenimine Conjugates as Biocompatible Carriers of CpG Oligodeoxynucleotides to Macrophages	3
Enzymatic synthesis of nylon precursors by 4-aminobutyrate aminotransferase and 6-oxohexanoate dehydrogenase	3
Optimized Sugar Extraction and Bioethanol Production from Lipid-extracted Sewage Sludge	3
Tremella fuciformis TFCUV5 Mycelial Culture-derived Exopolysaccharide Production and Its Anti-aging Effects on Skin Cells	3
Whole Cell-mediated Biocatalytic Synthesis of Helicid Cinnamylate and Its Biological Evaluation as a Novel Tyrosinase Inhibitor	3
Real-time, Economical Identification of Microplastics Using Impedance-based Interdigital Array Microelectrodes and k-Nearest Neighbor Model	3
Calculation of All Possible Stoichiometric Coefficients and Theoretical Yields of Microbial Global Reactions	3
Degradation of 1,4-dioxane by Newly Isolated Acinetobacter sp. M21 with Molasses as the Auxiliary Substrate	3
Erratum to: Utilization of Glucocorticoids as Additives for Enhanced Sialylation of Fc-fusion Protein in CHO Cell Cultures	3
Natural products ameliorating the adverse health effects by air particulate matter	3
Natural/Synthetic Polymer Materials for Bioink Development	3
Kinetic and Thermodynamic Study of the Ultrasonic Acetone-pentane Fractional Precipitation of Paclitaxel from the Plant Cell Cultures of Taxus chinensis	3
Highly Porous and Rigid, Full-thickness Human Skin Model from the Slime-webbed Fiber Scaffold	3
A deep learning framework for HbA1c levels assessment using short-term continuous glucose monitoring data	3
Growth-promoting Effect of Alginate Oligosaccharides on Rhodobacter sphaeroides	3
Harnessing balloon flower root-derived nanovesicles to mitigate hyperglycemia-induced cellular senescence	3
Xylose Fermentation Was Improved by Kluyveromyces marxianus KHM89 through Up-regulation of Nicotinamide Adenine Dinucleotide (NAD+) Salvage Pathway	3
Hsa_circ_0001165 Regulates the Malignant Phenotype and Angiogenesis of Prostate Cancer Cells through miR-654-3p/DDAH1	3
A neural ordinary differential equation model for predicting the growth of Chinese Hamster Ovary cell in a bioreactor system	3
Optimization of Process Parameters for Enhanced Production of Ranibizumab in Escherichia coli	3
Systems Biology Engineering of the Pantothenate Pathway to Enhance 3HB Productivity in Escherichia coli	3
Improvement of Sleeping Beauty Transposon System Enabling Efficient and Stable Protein Production	3
High Yield Fermentation of L-serine in Recombinant Escherichia coli via Co-localization of SerB and EamA through Protein Scaffold	3
Metal-polyphenol Complexes as Versatile Building Blocks for Functional Biomaterials	3
Terminalia chebula Medicinal Uses: A Review of in vitro and in vivo Studies	3
Use of endogenous acetate kinase for ATP regeneration system of whole-cell L-theanine production catalyzed by γ-glutamylmethylamide synthetase	3