Metabolic Engineering

Papers
(The TQCC of Metabolic Engineering is 22. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-03-01 to 2024-03-01.)
ArticleCitations
Metabolic engineering advances and prospects for amino acid production174
Towards bio-upcycling of polyethylene terephthalate147
Metabolic engineering for the synthesis of polyesters: A 100-year journey from polyhydroxyalkanoates to non-natural microbial polyesters136
Machine learning for metabolic engineering: A review132
Engineering biosynthesis of polyhydroxyalkanoates (PHA) for diversity and cost reduction132
CRISPR/Cas9 directed editing of lycopene epsilon-cyclase modulates metabolic flux for β-carotene biosynthesis in banana fruit131
Metabolic engineering for the production of dicarboxylic acids and diamines101
Replacing the Calvin cycle with the reductive glycine pathway in Cupriavidus necator92
Dynamic control in metabolic engineering: Theories, tools, and applications90
Building microbial factories for the production of aromatic amino acid pathway derivatives: From commodity chemicals to plant-sourced natural products78
In vitro prototyping of limonene biosynthesis using cell-free protein synthesis77
Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals75
Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity74
Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT244074
Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT244072
Coupling metabolic addiction with negative autoregulation to improve strain stability and pathway yield67
Engineering the oleaginous yeast Yarrowia lipolytica for high-level resveratrol production67
A guide to metabolic flux analysis in metabolic engineering: Methods, tools and applications65
Engineering chimeric diterpene synthases and isoprenoid biosynthetic pathways enables high-level production of miltiradiene in yeast63
Engineered Pseudomonas putida simultaneously catabolizes five major components of corn stover lignocellulose: Glucose, xylose, arabinose, p-coumaric acid, and acetic acid62
An optimized methanol assimilation pathway relying on promiscuous formaldehyde-condensing aldolases in E. coli62
Metabolic engineering of Saccharomyces cerevisiae for the de novo production of psilocybin and related tryptamine derivatives60
Targeting pathway expression to subcellular organelles improves astaxanthin synthesis in Yarrowia lipolytica59
Valorization of CO2 through lithoautotrophic production of sustainable chemicals in Cupriavidus necator59
Stress-driven dynamic regulation of multiple tolerance genes improves robustness and productive capacity of Saccharomyces cerevisiae in industrial lignocellulose fermentation57
Development of a growth coupled and multi-layered dynamic regulation network balancing malonyl-CoA node to enhance (2S)-naringenin biosynthesis in Escherichia coli56
Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin56
Combining genetically-encoded biosensors with high throughput strain screening to maximize erythritol production in Yarrowia lipolytica55
Elucidation of the complete biosynthetic pathway of the main triterpene glycosylation products of Panax notoginseng using a synthetic biology platform54
Engineering yeast subcellular compartments for increased production of the lipophilic natural products ginsenosides53
A single-host fermentation process for the production of flavor lactones from non-hydroxylated fatty acids52
Direct production of commodity chemicals from lignocellulose using Myceliophthora thermophila52
The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future51
Enzyme capacity-based genome scale modelling of CHO cells50
High-yield production of L-valine in engineered Escherichia coli by a novel two-stage fermentation48
Metabolism of syringyl lignin-derived compounds in Pseudomonas putida enables convergent production of 2-pyrone-4,6-dicarboxylic acid47
Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction47
Rebalancing microbial carbon distribution for L-threonine maximization using a thermal switch system47
Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system46
What CHO is made of: Variations in the biomass composition of Chinese hamster ovary cell lines46
Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers46
Metabolic design for selective production of nicotinamide mononucleotide from glucose and nicotinamide46
Engineering Saccharomyces cerevisiae for high yield production of α-amyrin via synergistic remodeling of α-amyrin synthase and expanding the storage pool46
Revealing metabolic mechanisms of interaction in the anaerobic digestion microbiome by flux balance analysis45
Biosynthesis of functional polyhydroxyalkanoates by engineered Halomonas bluephagenesis45
Direct biosynthesis of adipic acid from lignin-derived aromatics using engineered Pseudomonas putida KT244043
Green production of silybin and isosilybin by merging metabolic engineering approaches and enzymatic catalysis43
Systems metabolic engineering of Vibrio natriegens for the production of 1,3-propanediol42
Simultaneous secretion of seven lignocellulolytic enzymes by an industrial second-generation yeast strain enables efficient ethanol production from multiple polymeric substrates41
Cell-free styrene biosynthesis at high titers41
Tailoring cyanobacteria as a new platform for highly efficient synthesis of astaxanthin41
Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy41
Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida40
Engineering Escherichia coli for production of geraniol by systematic synthetic biology approaches and laboratory-evolved fusion tags39
Metabolic engineering of Cupriavidus necator H16 for improved chemoautotrophic growth and PHB production under oxygen-limiting conditions39
Synergistic improvement of N-acetylglucosamine production by engineering transcription factors and balancing redox cofactors39
Production of moth sex pheromones for pest control by yeast fermentation38
Engineering carotenoid production in mammalian cells for nutritionally enhanced cell-cultured foods37
Automated engineering of synthetic metabolic pathways for efficient biomanufacturing37
Tailored biosynthesis of gibberellin plant hormones in yeast36
High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica36
Metabolic engineering of type II methanotroph, Methylosinus trichosporium OB3b, for production of 3-hydroxypropionic acid from methane via a malonyl-CoA reductase-dependent pathway35
Guiding stars to the field of dreams: Metabolically engineered pathways and microbial platforms for a sustainable lignin-based industry35
Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 794235
Enhancement of the flavone contents of Scutellaria baicalensis hairy roots via metabolic engineering using maize Lc and Arabidopsis PAP1 transcription factors35
Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP135
Engineering of the 2,3-butanediol pathway of Paenibacillus polymyxa DSM 36534
Engineering Saccharomyces cerevisiae for isoprenol production34
Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol34
Development of Klebsiella pneumoniae J2B as microbial cell factory for the production of 1,3-propanediol from glucose34
Engineering of Corynebacterium glutamicum for high-level γ-aminobutyric acid production from glycerol by dynamic metabolic control33
Integrated knowledge mining, genome-scale modeling, and machine learning for predicting Yarrowia lipolytica bioproduction33
K-FIT: An accelerated kinetic parameterization algorithm using steady-state fluxomic data33
Independent component analysis of E. coli's transcriptome reveals the cellular processes that respond to heterologous gene expression33
Combinatorial metabolic pathway assembly approaches and toolkits for modular assembly33
High-level sustainable production of the characteristic protopanaxatriol-type saponins from Panax species in engineered Saccharomyces cerevisiae33
Highly efficient production of L-homoserine in Escherichia coli by engineering a redox balance route33
Rewiring carbon flux in Escherichia coli using a bifunctional molecular switch33
Efficient production of oxidized terpenoids via engineering fusion proteins of terpene synthase and cytochrome P45032
In vivo evolutionary engineering of riboswitch with high-threshold for N-acetylneuraminic acid production32
INCA 2.0: A tool for integrated, dynamic modeling of NMR- and MS-based isotopomer measurements and rigorous metabolic flux analysis32
A hybrid mechanistic-empirical model for in silico mammalian cell bioprocess simulation31
Unlocking the biosynthesis of sesquiterpenoids from methane via the methylerythritol phosphate pathway in methanotrophic bacteria, using α-humulene as a model compound31
Comprehensive study on Escherichia coli genomic expression: Does position really matter?31
High efficient production of plant flavonoids by microbial cell factories: Challenges and opportunities31
Engineering Escherichia coli for methanol-dependent growth on glucose for metabolite production31
Constructing an ethanol utilization pathway in Escherichia coli to produce acetyl-CoA derived compounds30
Engineering Halomonas bluephagenesis for L-Threonine production30
Structure-guided reshaping of the acyl binding pocket of ‘TesA thioesterase enhances octanoic acid production in E. coli30
Promiscuous phosphoketolase and metabolic rewiring enables novel non-oxidative glycolysis in yeast for high-yield production of acetyl-CoA derived products30
Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida30
Heterologous production of the epoxycarotenoid violaxanthin in Saccharomyces cerevisiae30
Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae30
Enhanced 2′-Fucosyllactose production by engineered Saccharomyces cerevisiae using xylose as a co-substrate29
Cascaded valorization of brown seaweed to produce l-lysine and value-added products using Corynebacterium glutamicum streamlined by systems metabolic engineering29
A genetic toolbox for metabolic engineering of Issatchenkia orientalis29
Overexpression of native ORANGE (OR) and OR mutant protein in Chlamydomonas reinhardtii enhances carotenoid and ABA accumulation and increases resistance to abiotic stress29
Animal-free heme production for artificial meat in Corynebacterium glutamicum via systems metabolic and membrane engineering29
Improving CRISPR/Cas9-mediated genome editing efficiency in Yarrowia lipolytica using direct tRNA-sgRNA fusions28
Engineering adipic acid metabolism in Pseudomonas putida27
Development of a clostridia-based cell-free system for prototyping genetic parts and metabolic pathways27
Organic waste conversion through anaerobic digestion: A critical insight into the metabolic pathways and microbial interactions27
A modular autoinduction device for control of gene expression in Bacillus subtilis27
Optimization of heterologous Darobactin A expression and identification of the minimal biosynthetic gene cluster27
Engineering Pseudomonas putida for efficient aromatic conversion to bioproduct using high throughput screening in a bioreactor27
Engineering cyanobacteria as cell factories for direct trehalose production from CO227
Isobutanol production in Corynebacterium glutamicum: Suppressed succinate by-production by pckA inactivation and enhanced productivity via the Entner–Doudoroff pathway26
Transportome-wide engineering of Saccharomyces cerevisiae26
Recent advances in constraint and machine learning-based metabolic modeling by leveraging stoichiometric balances, thermodynamic feasibility and kinetic law formalisms26
Engineering promiscuity of chloramphenicol acetyltransferase for microbial designer ester biosynthesis26
Metabolic engineering for valorization of macroalgae biomass26
Engineering the permeability of Halomonas bluephagenesis enhanced its chassis properties26
Metabolic engineering of E. coli for pyocyanin production26
Rational engineering strategies for achieving high-yield, high-quality and high-stability of natural product production in actinomycetes25
Development of bifunctional biosensors for sensing and dynamic control of glycolysis flux in metabolic engineering25
Machine-learning from Pseudomonas putida KT2440 transcriptomes reveals its transcriptional regulatory network25
Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves25
Metabolic engineering of Escherichia coli W3110 for efficient production of homoserine from glucose25
Secretory production of spider silk proteins in metabolically engineered Corynebacterium glutamicum for spinning into tough fibers25
The yeast platform engineered for synthetic gRNA-landing pads enables multiple gene integrations by a single gRNA/Cas9 system25
Heterologous biosynthesis of lutein in S. cerevisiae enabled by temporospatial pathway control25
Regulated redirection of central carbon flux enhances anaerobic production of bioproducts in Zymomonas mobilis25
Characterization of aromatic acid/proton symporters in Pseudomonas putida KT2440 toward efficient microbial conversion of lignin-related aromatics24
Simultaneous tracers and a unified model of positional and mass isotopomers for quantification of metabolic flux in liver24
Engineering Halomonas bluephagenesis as a chassis for bioproduction from starch24
Control of IgG glycosylation in CHO cell perfusion cultures by GReBA mathematical model supported by a novel targeted feed, TAFE24
Biobased PET from lignin using an engineered cis, cis-muconate-producing Pseudomonas putida strain with superior robustness, energy and redox properties24
Recent advances toward the bioconversion of methane and methanol in synthetic methylotrophs24
Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate24
Hydrogen utilization by Methylocystis sp. strain SC2 expands the known metabolic versatility of type IIa methanotrophs24
Adaptive laboratory evolution of Escherichia coli lacking cellular byproduct formation for enhanced acetate utilization through compensatory ATP consumption24
Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production24
Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens23
Reprint of Organic waste conversion through anaerobic digestion: A critical insight into the metabolic pathways and microbial interactions23
Dynamic control over feedback regulatory mechanisms improves NADPH flux and xylitol biosynthesis in engineered E. coli23
A “push-pull-restrain” strategy to improve citronellol production in Saccharomyces cerevisiae23
CRISPR-derived genome editing technologies for metabolic engineering23
CRISPR-based metabolic pathway engineering23
Systems metabolic engineering of Corynebacterium glutamicum for high-level production of 1,3-propanediol from glucose and xylose23
Integrating thermodynamic and enzymatic constraints into genome-scale metabolic models22
Systematic identification of Ocimum sanctum sesquiterpenoid synthases and (−)-eremophilene overproduction in engineered yeast22
Four-carbon dicarboxylic acid production through the reductive branch of the open cyanobacterial tricarboxylic acid cycle in Synechocystis sp. PCC 680322
Single-gene regulated non-spore-forming Bacillus subtilis: Construction, transcriptome responses, and applications for producing enzymes and surfactin22
Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound22
Crabtree/Warburg-like aerobic xylose fermentation by engineered Saccharomyces cerevisiae22
High-throughput screening for high-efficiency small-molecule biosynthesis22
Metabolic engineering of β-oxidation to leverage thioesterases for production of 2-heptanone, 2-nonanone and 2-undecanone22
Protocatechuate overproduction by Corynebacterium glutamicum via simultaneous engineering of native and heterologous biosynthetic pathways22
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