Metabolic Engineering

Article	Citations
Editorial Board	86
Genome-wide CRISPR screening identifies genes in recombinant human embryonic kidney 293 cells for increased ammonia resistance	78
Introduction of acetyl-phosphate bypass and increased culture temperatures enhanced growth-coupled poly-hydroxybutyrate production in the marine cyanobacterium Synechococcus sp. PCC7002	76
Butyrate as a growth factor of Clostridium acetobutylicum	73
Synthetic metabolic pathways for conversion of CO2 into secreted short-to medium-chain hydrocarbons using cyanobacteria	70
Engineering Halomonas bluephagenesis for high-efficiency biosynthesis of pyruvate	69
Development of a thermophilic l-arabinose-inducible system in Acetivibrio thermocellus (Clostridium thermocellum)	67
Engineering Halomonas bluephagenesis for pilot production of terpolymers containing 3-hydroxybutyrate, 4-hydroxybutyrate and 3-hydroxyvalerate from glucose	63
Elucidation and engineering of Sphingolipid biosynthesis pathway in Yarrowia lipolytica for enhanced production of human-type sphingoid bases and glucosylceramides	62
Engineering sub-organelles of a diploid Saccharomyces cerevisiae to enhance the production of 7-dehydrocholesterol	59
Efficient production of (S)-limonene and geraniol in Saccharomyces cerevisiae through the utilization of an Erg20 mutant with enhanced GPP accumulation capability	59
Biosensor-informed engineering of Cupriavidus necator H16 for autotrophic D-mannitol production	59
Improved arginine production in Escherichia coli by harnessing the intracellular citrulline	58
Alternative routes for production of the drug candidate d-chiro-inositol with Corynebacterium glutamicum using endogenous or promiscuous plant enzymes	58
Heterologous biosynthesis of betanin triggers metabolic reprogramming in tobacco	57
Pan-reactome analysis of Streptomyces strains reveals association and disconnection between primary and secondary metabolism	56
Metabolic engineering of Escherichia coli for the production of two-carbon primary amines	55
Engineering a biosensor based high-throughput screening platform for high-yield caffeic acid production in Escherichia coli	54
Engineering Yarrowia lipolytica for sustainable ricinoleic acid production: A pathway to free fatty acid synthesis	53
Engineering carbon source division of labor for efficient α-carotene production in Corynebacterium glutamicum	53
Quantifying supply and demand in the pea aphid-Buchnera symbiosis reveals the metabolic Achilles’ heels of this interaction	52
Identification of a specific exporter that enables high production of aconitic acid in Aspergillus pseudoterreus	51
De novo 2′-fucosyllactose biosynthesis using glucose as the sole carbon source by multiple engineered Bacillus subtilis	51
Integrating metabolome dynamics and process data to guide cell line selection in biopharmaceutical process development	51
Inferring secretory and metabolic pathway activity from omic data with secCellFie	49

Biosynthesis of 12-aminododecanoic acid from biomass sugars	49
Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera)	48
13C-Metabolic flux analysis of 3T3-L1 adipocytes illuminates its core metabolism under hypoxia	48
Versatile xylose and arabinose genetic switches development for yeasts	47
Optimized production of concanamycins using a rational metabolic engineering strategy	46
The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity	44
Development of a Streptomyces-based system for facile thioholgamide library generation and analysis	43
Machine-learning guided elucidation of contribution of individual steps in the mevalonate pathway and construction of a yeast platform strain for terpenoid production	43
Engineering an SspB-mediated degron for novel controllable protein degradation	43
Coordinating caffeic acid and salvianic acid A pathways for efficient production of rosmarinic acid in Escherichia coli	42
Disruption of acyl-acyl carrier protein (acyl-ACP) synthetase in cyanobacteria impairs lipid remodeling as revealed by acyl-ACP measurements	42
Consolidated bioprocessing for bioethanol production by metabolically engineered cellulolytic fungus Myceliophthora thermophila	41
Consistent biosynthesis of D-glycerate from variable mixed substrates	41
Engineering diverse fatty acid compositions of phospholipids in Escherichia coli	40
Rethinking 13C-metabolic flux analysis – The Bayesian way of flux inference	40
Silk fibroin production in Escherichia coli is limited by a positive feedback loop between metabolic burden and toxicity stress	40
Auto-inducible synthetic pathway in E. coli enhanced sustainable indigo production from glucose	39
Metabolic engineering of Escherichia coli for N-methylserotonin biosynthesis	39
Unlocking lager's flavour palette by metabolic engineering of Saccharomyces pastorianus for enhanced ethyl ester production	39
COSMIC-dFBA: A novel multi-scale hybrid framework for bioprocess modeling	39
De novo production of 1,3-olein-2-palmitin (OPO) and 1-olein-2-palmitin-3-linolein (OPL) by multiplexed reconstruction of lipid metabolism in yeasts	38
Coupling cell growth and biochemical pathway induction in Saccharomyces cerevisiae for production of (+)-valencene and its chemical conversion to (+)-nootkatone	38
A novel step towards the heterologous biosynthesis of paclitaxel: Characterization of T1βOH taxane hydroxylase	38
Insights into the methanol utilization capacity of Y. lipolytica and improvements through metabolic engineering	38
Membrane transporter identification and modulation via adaptive laboratory evolution	38
Time-resolved, deuterium-based fluxomics uncovers the hierarchy and dynamics of sugar processing by Pseudomonas putida	37
Deep learning for NAD/NADP cofactor prediction and engineering using transformer attention analysis in enzymes	36
Functional genomic screening in Komagataella phaffii enabled by high-activity CRISPR-Cas9 library	35
A Pseudomonas taiwanensis malonyl-CoA platform strain for polyketide synthesis	35
A reactive species reactions module for integration into genome-scale metabolic models for improved insights: Application to cancer	35
Boosting titers of engineered triketide and tetraketide synthases to record levels through T7 promoter tuning	35
Metabolic engineering of commensal bacteria for gut butyrate delivery and dissection of host-microbe interaction	34
Reconstruction of arginine deiminase pathway sustains a higher-energy state in mammalian cells	34
Engineering and evolution of the complete Reductive Glycine Pathway in Saccharomyces cerevisiae for formate and CO2 assimilation	34
Engineering Rhodosporidium toruloides for production of 3-hydroxypropionic acid from lignocellulosic hydrolysate	34
Biosynthesis of diverse α,ω-diol-derived polyhydroxyalkanoates by engineered Halomonas bluephagenesis	34
Editorial Board	33
Selective microbial production of lacto-N-fucopentaose I in Escherichia coli using engineered α-1,2-fucosyltransferases	33
Self-controlled in silico gene knockdown strategies to enhance the sustainable production of heterologous terpenoid by Saccharomyces cerevisiae	33
Endogenous BiP reporter system for simultaneous identification of ER stress and antibody production in Chinese hamster ovary cells	33
Corrigendum to “Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440” (Metab. Eng. 67 (2021) 250–261)	33
Metabolic engineering of artificially modified transcription factor SmMYB36-VP16 for high-level production of tanshinones and phenolic acids	32
Systems metabolic engineering upgrades Corynebacterium glutamicum for selective high-level production of the chiral drug precursor and cell-protective extremolyte L-pipecolic acid	32
Reusable and modular combinatorial libraries for iterative metabolic engineering of Saccharomyces cerevisiae	32
Editorial Board	32
Targeting cofactors regeneration in methylation and hydroxylation for high level production of Ferulic acid	32
Hybrid biological-chemical strategy for converting polyethylene into a recyclable plastic monomer using engineered Corynebacterium glutamicum	32
Repurposing plant hormone receptors as chemically-inducible genetic switches for dynamic regulation in yeast	31
Systems metabolic engineering of Corynebacterium glutamicum eliminates all by-products for selective and high-yield production of the platform chemical 5-aminovalerate	30
Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae	30

Flux optimization using multiple promoters in Halomonas bluephagenesis as a model chassis of the next generation industrial biotechnology	30
Manufacturing specialized wax esters in plants	30
Stress-driven dynamic regulation of multiple genes to reduce accumulation of toxic aldehydes	30
Co-cultivation of Saccharomyces cerevisiae strains combines advantages of different metabolic engineering strategies for improved ethanol yield	30
Engineering of insect juvenile hormone III biosynthesis in the plant Nicotiana benthamiana	29
Efficient production of natural sunscreens shinorine, porphyra-334, and mycosporine-2-glycine in Saccharomyces cerevisiae	29
Ectoine hyperproduction by engineered Halomonas bluephagenesis	29
Electrical-biological hybrid system for carbon efficient isobutanol production	29
High-yield production of FK228 and new derivatives in a Burkholderia chassis	29
Evaluation of strategies to narrow the product chain-length distribution of microbially synthesized free fatty acids	29
Developing a Bacillus licheniformis platform for de novo production of γ-aminobutyric acid and other glutamate-derived chemicals	29
Systems metabolic engineering of Klebsiella pneumoniae for high-level 1,3-propanediol production	28
Biosynthesis of β-lactam nuclei in yeast	28
Sustainable production through spatial niche partitioning in engineered light-driven microbial community	28
Minimized antibiotic-free plasmid vector for gene therapy utilizing a new toxin-antitoxin system	28
KETCHUP: Parameterizing of large-scale kinetic models using multiple datasets with different reference states	28
Generation of a Vibrio-based platform for efficient conversion of raffinose through Adaptive Laboratory Evolution on a solid medium	28
Metabolic engineering of Acinetobacter baylyi ADP1 for efficient utilization of pentose sugars and production of glutamic acid	28
Editorial Board	28
Establishing heterologous betaxanthin pigment biosynthesis in cyanobacteria	27
Overexpression of peroxisome proliferator-activated receptor γ co-activator-1⍺ (PGC-1⍺) in Chinese hamster ovary cells increases oxidative metabolism and IgG productivity	27
Fluctuating pH for efficient photomixotrophic succinate production	27
Omics analyses decoding mechanisms underlying the self-flocculating phenotype of yeast cells and stress tolerance for robust production	27
Metabolic engineering of Escherichia coli for high production of 1,5-pentanediol via a cadaverine-derived pathway	26
Sustainable production of genistin from glycerol by constructing and optimizing Escherichia coli	26
Biomass accumulation in chondrocyte metabolic modelling: Incorporating extracellular matrix proxies to predict tissue engineering outcomes	26
Genetic and biocatalytic basis of formate dependent growth of Escherichia coli strains evolved in continuous culture	26
CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion	26
High-level and -yield production of L-leucine in engineered Escherichia coli by multistep metabolic engineering	26
Editorial Board	25
A tRNA Modification-based strategy for Identifying amiNo acid Overproducers (AMINO)	25
Engineering 5-hydroxymethylfurfural (HMF) oxidation in Pseudomonas boosts tolerance and accelerates 2,5-furandicarboxylic acid (FDCA) production	25
Engineering of Escherichia coli cytoplasm and periplasm for efficient synthesis of salvianic acid A	25
Genome mining of tailoring enzymes from biosynthetic gene clusters for synthetic biology: A case study with fungal methyltransferases	25
Metabolic engineering of Corynebacterium glutamicum for increased cis, cis-muconate production from plant-derived p-hydroxycinnamates via deregulated pathway flux and increased CoA intermediate availa	25
Laboratory evolution of Synechocystis sp. PCC 6803 for phenylpropanoid production	25
Evolution-assisted engineering of E. coli enables growth on formic acid at ambient CO2 via the Serine Threonine Cycle	25
A dynamic kinetic model captures cell-free metabolism for improved butanol production	24
Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system	24
Demonstration and technoeconomic analysis of dodecanol production from acetate using metabolically engineered Escherichia coli	24
Rational search of genetic design space for a heterologous terpene metabolic pathway in Streptomyces	24
Proteome trade-off between primary and secondary metabolism shapes acid stress induced bacterial exopolysaccharide production	24
Coordinated reprogramming of ATP metabolism strongly enhances adipic acid production in Escherichia coli	23
A tunable metabolic valve for precise growth control and increased product formation in Pseudomonas putida	23
Laboratory evolution reveals general and specific tolerance mechanisms for commodity chemicals	23
Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction	23
Engineered non-canonical reductive TCA pathway drives high-yield succinic acid biosynthesis in Yarrowia lipolytica	23
Thermodynamic limitations of PHB production from formate and fructose in Cupriavidus necator	23
Encapsulation of select violacein pathway enzymes in the 1,2-propanediol utilization bacterial microcompartment to divert pathway flux	22
dCas9-mediated dysregulation of gene expression in human induced pluripotent stem cells during primitive streak differentiation	22
Cyanobacterial phycobilisomes as a platform for the stable production of heterologous enzymes and other proteins	22
Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida	22
AI-based automated construction of high-precision Geobacillus thermoglucosidasius enzyme constraint model	22
Corrigendum to “Microbial production of propionic acid through a novel β-alanine route” [Metabol. Eng. (2026) 219–231 93]	22
Development of an efficient yeast platform for cannabigerolic acid biosynthesis	22
Ribo-seq guided design of enhanced protein secretion in Komagataella phaffii	21
Engineering xylose catabolism in the yeast Komagataella phaffii	21
Multi-dimensional experimental and computational exploration of metabolism pinpoints complex probiotic interactions	21
Engineering Escherichia coli for the production of saturated archaeal lipids	21
PET-FBA: A lightweight enzyme allocation and thermodynamics-constrained flux analysis approach to explore Escherichia coli metabolic adaptation to intracellular acidification	21
Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation	21
Enabling anaerobic growth of Escherichia coli on glycerol in defined minimal medium using acetate as redox sink	21
Engineered plants for the production of the antioxidants arbutin and gallate	21
Engineering ethanologenicity into the extremely thermophilic bacterium Anaerocellum (f. Caldicellulosiriuptor) bescii	21
Multi-omic based production strain improvement (MOBpsi) for bio-manufacturing of toxic chemicals	21
Modulating fatty acid metabolism and composition of CHO cells by feeding high levels of fatty acids complexed using methyl-β-cyclodextrin	21
Metabolic engineering of Corynebacterium glutamicum for vitamin B12-independent production of 3-hydroxypropionic acid	20
Engineering nucleotide sugar synthesis pathways for independent and simultaneous modulation of N-glycan galactosylation and fucosylation in CHO cells	20
A nutrition algorithm to optimize feed and medium composition using genome-scale metabolic models	20
Population genomics-guided engineering of phenazine biosynthesis in Pseudomonas chlororaphis	20
Metabolic engineering of human gut microbiome: Recent developments and future perspectives	20
Multi-omics view of recombinant Yarrowia lipolytica: Enhanced ketogenic amino acid catabolism increases polyketide-synthase-driven docosahexaenoic production to high selectivity at the gram scale	20
Establishing a coumarin production platform by protein and metabolic engineering	20
Engineering metabolic flux for the microbial synthesis of aromatic compounds	20
Editorial Board	20
Enhancing oil feedstock utilization for high-yield low-carbon polyhydroxyalkanoates industrial bioproduction	20
Corrigendum to “Metabolic and enzyme rewiring enables high-production of vanillin in unconventional yeast” [Metabol. Eng. 93 (2026) 158–167]	20
Advances in metabolic engineering of Vibrio natriegens as an unconventional host for biotechnology	20
Big data and machine learning: Metabolic engineering special issue	20
Accurate prediction of in vivo protein abundances by coupling constraint-based modelling and machine learning	20

Metabolic impact of heterologous protein production in Pseudomonas putida: Insights into carbon and energy flux control	20
Combinatorial iterative method for metabolic engineering of Yarrowia lipolytica: Application for betanin biosynthesis	20
Metabolic engineering improves transduction efficiency and downstream vector isolation by altering the lipid composition of extracellular vesicle-enclosed AAV	19
Engineering Escherichia coli for efficient glutathione production	19
Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol	19
Metabolic reprogramming and biosensor-assisted mutagenesis screening for high-level production of L-arginine in Escherichia coli	19
Production of species-specific anthocyanins through an inducible system in plant hairy roots	19
Evaluating proteome allocation of Saccharomyces cerevisiae phenotypes with resource balance analysis	19
Dynamic regulation combined with systematic metabolic engineering for high-level palmitoleic acid accumulation in oleaginous yeast	19
Beyond CEN.PK - parallel engineering of selected S. cerevisiae strains reveals that superior chassis strains require different engineering approaches for limonene production	18
Deciphering molecular drivers of lactate metabolic shift in mammalian cell cultures	18
Bioconversion of CO2 into valuable bioproducts via synthetic modular co-culture of engineered Chlamydomonas reinhardtii and Escherichia coli	18
Strategic engineering for overproduction of oviedomycin, a Type II polyketide, in Escherichia coli	18
Editorial Board	18
A contribution of metabolic engineering to addressing medical problems: Metabolic flux analysis	18
Improving the soluble expression of difficult-to-express proteins in prokaryotic expression system via protein engineering and synthetic biology strategies	18
Metabolic engineering of Escherichia coli for uridine-5′-diphosphate N-acetylglucosamine production through salvage pathway	18
Suppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism	18
High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space	18
Lipid accumulation in nitrogen and phosphorus-limited yeast is caused by less growth-related dilution	18
One-pot selective biosynthesis of Tyrian purple in Escherichia coli	17
Microbial production of creatine using growth-coupled selection systems	17
Efforts to install a heterologous Wood-Ljungdahl pathway in Clostridium acetobutylicum enable the identification of the native tetrahydrofolate (THF) cycle and result in early induction of solvents	17
Editorial Board	17
Optogenetic control of Cdc48 for dynamic metabolic engineering in yeast	17
High glutamate demand enables simultaneous consumption of glycerol and citrate despite carbon catabolite repression in engineered Bacillus subtilis strains	17
Engineering oleaginous yeast Rhodotorula toruloides for production of alkanes and alkenes	16
Tailoring Escherichia coli for high-yield production of O-acetyl-L-homoserine through multi-node metabolic regulation	16
Adaptive laboratory evolution and genetic engineering improved terephthalate utilization in Pseudomonas putida KT2440	16
Metabolic engineering of Escherichia coli for the high-level production of putrescine	16
Establishing an Autonomous Cascaded Artificial Dynamic (AutoCAD) regulation system for improved pathway performance	16
Development of Corynebacterium glutamicum as a monoterpene production platform	16
Edible fungus Fusarium venenatum: advances, challenges, and engineering strategies for future food production	16
Improved biosynthesis of C4 derivatives by engineered thiolase	16
Modular, synthetic chromosomes as new tools for large scale engineering of metabolism	16
Multi-omics driven genome-scale metabolic modeling improves viral vector yield in HEK293	16
Designing glucose utilization "highway" for recombinant biosynthesis	15
Construction of a synthetic metabolic pathway for biosynthesis of threonine from ethylene glycol	15
Genome-scale models of metabolism and expression predict the metabolic burden of recombinant protein expression	15
Efficient production of an antitumor precursor actinocin and other medicinal molecules from kynurenine pathway in Escherichia coli	15
Initiation of fatty acid biosynthesis in Pseudomonas putida KT2440	15
Evolution-guided tolerance engineering of Pseudomonas putida KT2440 for production of the aviation fuel precursor isoprenol	15
A multilayered biocontainment system for laboratory and probiotic yeast	15
Metabolic division engineering of Escherichia coli consortia for de novo biosynthesis of flavonoids and flavonoid glycosides	15
Precise biosynthesis of β-1,2-glucan from cellulosic materials by in vitro metabolic engineering	15
Metabolic engineering of Halomonas bluephagenesis for production of five carbon molecular chemicals derived from L-lysine	15
Cross-regulation between proteome reallocation and metabolic flux redistribution governs bacterial growth transition kinetics	15
De novo biosynthesis of 3-hydroxy-3-methylbutyrate as anti-catabolic supplement by metabolically engineered Escherichia coli	15
Engineering yeast for high-level production of diterpenoid sclareol	15
Metabolic engineering in Hot Acid: Strategies enabling chemolithotrophy in thermoacidophilic archaea	15
A genome-scale dynamic constraint-based modelling (gDCBM) framework predicts growth dynamics, medium composition and intracellular flux distributions in CHO clonal variations	15
Development of a salt-enhanced promoter strategy for activating silent biosynthetic gene clusters from streptomycetes	14
Redefining HexR regulatory landscape in Pseudomonas putida KT2440 through integrative systems biology	14
Rank-ordering of known enzymes as starting points for re-engineering novel substrate activity using a convolutional neural network	14
Directed evolution of biomass intensive CHO cells by adaptation to sub-physiological temperature	14
BGC heteroexpression strategy for production of novel microbial secondary metabolites	14
Corrigendum to “Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440” [Metab. Eng. 59 (2020) 64–75]	14
Switching the yeast metabolism via manipulation of sugar phosphorylation	14
Improved terephthalic acid production from p-xylene using metabolically engineered Pseudomonas putida	14
Reconstructing curcumin biosynthesis in yeast reveals the implication of caffeoyl-shikimate esterase in phenylpropanoid metabolic flux	14
Engineering low-salt growth Halomonas Bluephagenesis for cost-effective bioproduction combined with adaptive evolution	14
Rational design of a bacterial import system for new-to-nature molecules	14
Enhancing the production of isopropanol with reduced CO2 emission via protein and metabolic engineering using Corynebacterium glutamicum	14
Engineering the cellulolytic bacterium, Clostridium thermocellum, to co-utilize hemicellulose	14
Exploring the versatility of fatty acid biosynthesis in Escherichia coli: Production of random methyl branched fatty acids	14
Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme	13
Plastidial engineering with coupled farnesyl diphosphate pool reconstitution and enhancement for sesquiterpene biosynthesis in tomato fruit	13
Systematic rewiring of Bacillus subtilis for efficient de novo biosynthesis of the neuroprotectant cytidine-5′-diphosphocholine	13
A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways	13
Deciphering the network of cholesterol biosynthesis in Paris polyphylla laid a base for efficient diosgenin production in plant chassis	13
Enzyme activity engineering based on sequence co-evolution analysis	13
Production of cholesterol-like molecules impacts Escherichia coli robustness, production capacity, and vesicle trafficking	13
Modularized Engineering of Shewanella oneidensis MR-1 for Efficient and Directional Synthesis of 5-Aminolevulinic Acid	13
Riboswitch-guided chalcone synthase engineering and metabolic flux optimization for enhanced production of flavonoids	13
Live isolation of naïve ESCs via distinct glucose metabolism and stored glycogen	13
Evaluation of enzyme-constrained genome-scale model through metabolic engineering of anaerobic co-production of 2,3-butanediol and glycerol by Saccharomyces cerevisiae	13
The halo of future bio-industry based on engineering Halomonas	13
Design and application of a kinetic model of lipid metabolism in Saccharomyces cerevisiae	13
Evolution and engineering of pathways for aromatic O-demethylation in Pseudomonas putida KT2440	13
Engineering phenylpyruvate decarboxylase for controlled biosynthesis of aromatic amino acid derivatives	13
A long-term growth stable Halomonas sp. deleted with multiple transposases guided by its metabolic network model Halo-ecGEM	12
Expression in CHO cells of a bacterial biosynthetic pathway producing a small non-ribosomal peptide aldehyde prevents proteolysis of recombinant proteins	12
Reconstruction of a resource balance analysis model of Clostridium thermocellum examines the metabolic cost of glycolytic and cellulosome enzymes	12
Engineering the biological conversion of formate into crotonate in Cupriavidus necator	12
Plasmid-based electroporation for efficient genetic engineering in immortalized T lymphocytes	12
Guidelines for extracting biologically relevant context-specific metabolic models using gene expression data	12
Development of a nonauxotrophic L-homoserine hyperproducer in Escherichia coli by systems metabolic engineering	12
De novo biosynthesis of D-panthenol in engineered E. coli with rationally designed L-homoserine decarboxylase	12
Unleashing the innate ability of Escherichia coli to produce D-Allose	12
Data-driven and model-guided systematic framework for media development in CHO cell culture	12
Mechanism and engineering of endoplasmic reticulum-localized membrane protein folding in Saccharomyces cerevisiae	12
Combinatorial gene inactivation of aldehyde dehydrogenases mitigates aldehyde oxidation catalyzed by E. coli resting cells	12
Metabolic engineering of the acid-tolerant yeast Pichia kudriavzevii for efficient L-malic acid production at low pH	12
Serial adaptive laboratory evolution enhances mixed carbon metabolic capacity of Escherichia coli	12
The 6-phosphofructokinase reaction in Acetivibrio thermocellus is both ATP- and pyrophosphate-dependent	12