Nature Biotechnology

Article	Citations
Forum: Alexandrov and Le	1450
Federation Bio: from single strains to a purely synthetic microbiome	1024
People	959
Forum: Optogenetics roundtable with Deisseroth and Gradinaru	800
Multiplexed mapping of chromatin features at single-cell resolution	623
First Rounders: Jay Keasling	558
Synthetic GPCRs for controlling cellular responses	498
Base editors	483
Forum: CRISPR screening roundtable with Stegmaier and Doench	424
People	419
Biotech news from around the world	415
Bilateral China–Brazil ties over agbiotech	406
Bluebird’s CALD gene therapy poised for approval	404
Biotech news from around the world	377
Bat biotech takes flight	371
Biotech news from around the world	346
Diffusion model expands RoseTTAFold’s power	344
Fungal architecture is a thing, and it’s eye-catching	336
Engineered Cas13 variants with minimal collateral RNA targeting	326
People	320
Linking genetic variants to cellular function and disease	299
Ultra-precision medicine	295
Photys Therapeutics: customizing phosphorylation via molecular matchmaking	281
First Rounders: Lita Nelsen	281
The champions of drug development	275

Putting CRISPR into African hands to future-proof crops	265
Women build strength in numbers	261
Synthetic biologics provide a path to target bHLH transcription factors	260
Unlocking drug modes of action with multi-dimensional high-throughput metabolic profiling	257
A neural network for long-term super-resolution imaging of live cells with reliable confidence quantification	256
Increase vigilance against cyberattacks	254
Patents benefit patients and patent reform would spur diagnostic and therapeutic development	253
Biotech news from around the world	232
Vector BioPharma: resurrecting adenoviral gene delivery	230
Multiplex-GAM detects complex chromatin interactions	229
Fluorescence microscopy resolution increases to near-atomic level	229
Eight diseases for gene therapy accelerator	228
People	225
Wearable devices	224
Decoding the building blocks of cellular processes from single-cell transcriptomics data	217
Editor’s pick: Tenpoint Therapeutics	217
Spanish tortillas go vegan	216
HIV drug 100% protective	214
A base editor modifies bacterial genes in the guts of living mice	214
Personalized medicine is having its day	212
Biotech news from around the world	211
Protein design: the experts speak	207
Radical solutions	205
Big pharma craves slice of AI-based RNA drug discovery	201
Monitoring protein proximity with fluorogens	196
Nanopore sequencing	190
Base editing marches on the clinic	189
Detecting somatic mutations in single-cell data sets	177
Precise intraoperative brain tumor classification	176
Startups probe hidden viruses in the ‘dark genome’ to treat disease	170
An improved organoid model of the human cerebellum	170
Merck enlists trispecific killers	168
Cancer Moonshot aims at liquid biopsies	167
Single-cell multi-omics allows functional characterization of structural variants	164
AI’s plastic recycling	162
FDA okays first cardiac myosin inhibitor	158
Biotech news from around the world	157
Citizen chickpeas	154
100,000 genomes — in Africa, for Africa	152
Single-cell RNA-seq keeps cells alive	152
Vision–language AI assistance in human pathology	152
Biotech news from around the world	151
People	149
Has the PTAB made a difference in drug settlements and generic entry?	148
In vivo CRISPR agent cuts HAE attacks 95%	147
Supercharging T cell therapy with cancer mutations	145
Single-molecule peptide sequencing on semiconductor chips	144
Exploring tRNAs and their modifications and crosstalk using Nano-tRNAseq	142
Around the world in a month	141
Lentiviral vector cleared of causing blood cancer	137

Five questions with Weixin Tang	136
Editor’s pick: Switch Therapeutics	136
Cellular shuttles put mislocalized proteins in their right place	135
T cells	135
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	133
An antigen discovery pipeline integrates multi-omics data and informs immunotherapy	133
Uncovering the global RNA virome	131
Government targets, end-of-year patenting rush and innovative performance in China	130
Industry moves on 3Rs	129
People	128
Machine learning in drug discovery	127
Organoids and organs on a chip	127
Spatial characterization of single tumor cells by proteomics	127
Denmark invests in mucosal vaccines	126
Mining extinct proteomes for antimicrobial peptides	125
Toward a safer and more secure US bioeconomy	125
Glia-enriched cortical organoids implanted in mice capture astrocyte diversity	124
Making space for spatial biology in the clinic	122
Capturing and modeling cellular niches from dissociated single-cell and spatial data	121
Video game unleashes millions of citizen scientists on microbiome research	121
Sound healing and beyond	119
CRISPR-free, strand-selective mitochondrial DNA base editing using a nickase	115
AI for drug discovery is booming, but who owns the patents?	114
Wireless microelectronic fibers to discover gut and brain pathways	113
Chroma Medicine and Tune Therapeutics: Two companies take up epigenome editing	112
Identification of antimicrobial peptides from the human gut microbiome using deep learning	112
CRISPR therapies march into clinic, but genotoxicity concerns linger	111
Club-goers take first bites of lab-made chicken	111
Startup tackles oncogene-rich ecDNA	111
The lysosomal degraders	110
Inferring cell–cell communication at single-cell resolution	110
RNA drugs lower lipoprotein(a) and genetically driven cholesterol	110
Prime editing using CRISPR-Cas12a and circular RNAs in human cells	108
Genotoxic effects of base and prime editing in human hematopoietic stem cells	108
A spatial genome aligner for resolving chromatin architectures from multiplexed DNA FISH	108
Unidirectional single-file transport of full-length proteins through a nanopore	108
Real-time monitoring of tumor-homing bacteria and tumor cells in vivo using ultrasound	108
Massively parallel phenotyping of coding variants in cancer with Perturb-seq	107
Quantifying bias introduced by sample collection in relative and absolute microbiome measurements	107
Single-cell m6A mapping in vivo using picoMeRIP–seq	106
Survival of the fittest glia	105
The digital and analog worlds of protein engineering	105
Expanding access to CAR T cell therapies through local manufacturing	104
Integration of spatial and single-cell data across modalities with weakly linked features	104
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	104
Imaging glycosylated RNAs at the subcellular scale	102
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	102
Author Correction: Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning	102
KARR-seq reveals cellular higher-order RNA structures and RNA–RNA interactions	102
Identification of mobile genetic elements with geNomad	102
The global patent landscape of artificial intelligence applications for cancer	101
Do patent applications and Cooperative Research and Development Agreements between the National Cancer Institute and industry serve the public interest?	101
Soil microbiome engineering for sustainability in a changing environment	101
Supervised discovery of interpretable gene programs from single-cell data	100
Activation of recombinases at specific DNA loci by zinc-finger domain insertions	99
An optimized toolkit for prime editing	98
Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells	97
Whole-body cellular mapping in mouse using standard IgG antibodies	96
A new mass analyzer shakes up the proteomics field	94
An oncolytic virus–T cell chimera for cancer immunotherapy	94
The prevalence of drug patent term extensions in the United States, 2000–2018	94
Improving microbial phylogeny with citizen science within a mass-market video game	93
Contracts and intellectual property rights in translational R&D: furthering safeguards in the public interest	92
Designing proteins with language models	92
Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data	91
Evolutionary gambit to defeat drug resistance in cancer	90
One-step nanoscale expansion microscopy reveals individual protein shapes	89
Engineered serum markers for non-invasive monitoring of gene expression in the brain	88
Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap	88
Near-cognate tRNAs increase the efficiency and precision of pseudouridine-mediated readthrough of premature termination codons	87
Author Correction: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion	86
Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations	85
The rise of patient avatars in precision oncology	84
Generative models for protein structures and sequences	83
Publisher Correction: Multistate and functional protein design using RoseTTAFold sequence space diffusion	82
Sparks of function by de novo protein design	82
Author Correction: Quantifying bias introduced by sample collection in relative and absolute microbiome measurements	82
Trans-nuclease activity of Cas9 activated by DNA or RNA target binding	81
Long-term intravital subcellular imaging with confocal scanning light-field microscopy	81
Improving the predictive power of mouse models	81

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	80
Chemical and topological design of multicapped mRNA and capped circular RNA to augment translation	79
Rapid generation of long, chemically modified pegRNAs for prime editing	79
A developmental route to hematopoietic stem cells	79
Accessible training for academic clinicians and researchers to address the need for academic entrepreneurs	79
After obesity drugs’ success, companies rush to preserve skeletal muscle	78
The community of the DAO	78
The next giant step for microbes	77
First herpesvirus gene therapy	77
Predicting disease variants using biodiversity and machine learning	76
Dreaming ideal protein structures	76
Multiplex de Bruijn graphs enable genome assembly from long, high-fidelity reads	74
CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA	74
Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro	74
Personalized phosphoproteomics identifies functional signaling	74
Integrating innovation as a core objective in medical training	73
A knowledge graph to interpret clinical proteomics data	73
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	72
A Python library for probabilistic analysis of single-cell omics data	71
RNA delivery with a human virus-like particle	71
Estimation of tumor cell total mRNA expression in 15 cancer types predicts disease progression	71
European patent protection for medical uses of known products and drug repurposing	71
Publisher Correction: Integrative spatial analysis of cell morphologies and transcriptional states with MUSE	71
Identifying synergistic high-order 3D chromatin conformations from genome-scale nanopore concatemer sequencing	70
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	70
Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH	70
Transplantation of a human liver following 3 days of ex situ normothermic preservation	70
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	69
Helping others enhances graduate student wellness and mental health	69
Bioengineered corneal tissue for minimally invasive vision restoration in advanced keratoconus in two clinical cohorts	69
Fludarabine increases nuclease-free AAV- and CRISPR/Cas9-mediated homologous recombination in mice	69
Author Correction: Multiscale and integrative single-cell Hi-C analysis with Higashi	69
Enhanced cancer detection from cell-free DNA	69
Variant to function mapping at single-cell resolution through network propagation	68
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	68
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	68
A multiplex implantable microdevice assay identifies synergistic combinations of cancer immunotherapies and conventional drugs	68
Mapping mRNA modifications for functional studies	68
High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs	67
What the COVID-19 pandemic revealed about intellectual property	66
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	66
Solid-phase capture and profiling of open chromatin by spatial ATAC	65
Wireless, closed-loop, smart bandage with integrated sensors and stimulators for advanced wound care and accelerated healing	65
Dynamic, adaptive sampling during nanopore sequencing using Bayesian experimental design	65
A proteome-wide atlas of drug mechanism of action	64
Novel recombinases for large DNA insertions	64
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	63
Large language models generate functional protein sequences across diverse families	63
scChIX-seq infers dynamic relationships between histone modifications in single cells	63
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	62
Drug licensing as evidence of evolution, diffusion and catch-up in East Asia	62
Protein structure prediction with in-cell photo-crosslinking mass spectrometry and deep learning	62
Spatial mapping of the total transcriptome by in situ polyadenylation	62
gutSMASH predicts specialized primary metabolic pathways from the human gut microbiota	62
Writing cellular history in protein chains	62
Chem-map profiles drug binding to chromatin in cells	61
Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms	61
High-throughput retrieval of target sequences from complex clone libraries using CRISPRi	60
Rapid-response manufacturing of adenovirus-vectored vaccines	60
Efficient evolution of human antibodies from general protein language models	59
Mapping the patent landscape of medical machine learning	59
CRISPR-edited plants by grafting	59
Efficient engineering of human and mouse primary cells using peptide-assisted genome editing	59
Pangenome graph construction from genome alignments with Minigraph-Cactus	59
A microneedle vaccine printer for thermostable COVID-19 mRNA vaccines	59
Evaluating the analytical validity of circulating tumor DNA sequencing assays for precision oncology	58
Democratizing knowledge representation with BioCypher	58
Strand-selective base editing of human mitochondrial DNA using mitoBEs	58
A technical approach to global plant genome editing regulation	57
High-efficiency prime editing with optimized, paired pegRNAs in plants	57
A unified haplotype-based method for accurate and comprehensive variant calling	57
Ovarian tumor cells gain competitive advantage by actively reducing the cellular fitness of microenvironment cells	57
Prediction of drug efficacy from transcriptional profiles with deep learning	56
Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing	56
More than just content: building community in the graduate classroom	56
Monitoring deep-tissue oxygenation with a millimeter-scale ultrasonic implant	56
Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice	55
Deletion and replacement of long genomic sequences using prime editing	55
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	55
Negative innovation: when patents are bad for patients	55
A small and highly sensitive red/far-red optogenetic switch for applications in mammals	55
Ebola as a case study for the patent landscape of medical countermeasures for emerging infectious diseases	55
Author Correction: Engineered pegRNAs improve prime editing efficiency	54
Fast and accurate metagenotyping of the human gut microbiome with GT-Pro	54
Droplet-based transcriptome profiling of individual synapses	54
NINDS launches network to develop treatments for ultra-rare neurological diseases	54
Single-cell immunology of SARS-CoV-2 infection	54
Robust integration of multiple single-cell RNA sequencing datasets using a single reference space	54
Improving combination drug trials using ‘definitive screening designs’	53
DNA writing technologies moving toward synthetic genomes	53
How to grow (almost) anything: a hybrid distance learning model for global laboratory-based synthetic biology education	53
Transcriptome-wide profiling and quantification of N6-methyladenosine by enzyme-assisted adenosine deamination	53
Framework for predicting alloreactivity in hematopoietic cell transplants	53
People	52
Virus-like elements	52