Nature Biotechnology

Article	Citations
Single-cell RNA-seq keeps cells alive	3001
Fungal architecture is a thing, and it’s eye-catching	2148
Fluorescence microscopy resolution increases to near-atomic level	1652
Synthetic biologics provide a path to target bHLH transcription factors	913
AI’s plastic recycling	890
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	775
People	761
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	644
Denmark invests in mucosal vaccines	625
Wireless microelectronic fibers to discover gut and brain pathways	599
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	568
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	509
Framework for predicting alloreactivity in hematopoietic cell transplants	448
Bite-sized solutions	429
An optimized toolkit for prime editing	419
Design of optimized epigenetic regulators for durable gene silencing with application to PCSK9 in nonhuman primates	400
Programmable promoter editing for precise control of transgene expression	394
Author Correction: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew	389
People	385
Orchestration of secondary metabolite production in Streptomyces species	383
Cellular shuttles put mislocalized proteins in their right place	372
Diffusion model expands RoseTTAFold’s power	365
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	352
Democratizing knowledge representation with BioCypher	347
Dreaming ideal protein structures	327

Variant to function mapping at single-cell resolution through network propagation	312
Engineered serum markers for non-invasive monitoring of gene expression in the brain	308
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	303
Cancer Moonshot aims at liquid biopsies	293
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	291
Generative models for protein structures and sequences	277
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	266
Droplet-based transcriptome profiling of individual synapses	261
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	259
An oncolytic virus–T cell chimera for cancer immunotherapy	257
Chem-map profiles drug binding to chromatin in cells	246
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	228
The lysosomal degraders	224
Biopharma dives into tumor-seeking radioactive drugs	222
Dual SORT LNPs for multi-organ base editing	221
The world’s first CRISPR therapy is approved: who will receive it?	211
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	208
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	207
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	207
Behind the graduate mental health crisis in science	206
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	199
Engineering innate immune cells for cancer immunotherapy	193
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	191
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	190
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	188
Live cell imaging of signaling networks using a conventional microscope	186
AI predicts cancer relapse from slides	186
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	186
Biotech news from around the world	185
The need for need-finding in medical education	183
3Q22 — more malaise in the markets	183
Author Correction: The cost of drug patent expiration date errors	181
Biotech news from around the world	178
People	176
Gains in early-onset dementia with progranulin open new paths for drug discovery	175
HSV-1’s contribution as a vector for gene therapy	174
Who will oversee the ethical limits of human embryo research?	174
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	172
Slaughter-free meat hits the grocery shelf	171
Cancer diagnostics	171
Delix Therapeutics : psychedelics without the trip	170
Towards the non-invasive imaging of brain networks and functions at high resolution	169
Precise DNA cleavage using CRISPR-SpRYgests	169
Orna Therapeutics: circular logic	168
Computationally designed enzymes show potent catalytic activity	167
Adrestia Therapeutics — Gene networks to the rescue	167
Decoding gene regulation with CRISPR perturbations	166
Hallucinating functional protein sequences	162
Next-generation psychedelics: should new agents skip the trip?	161
FDA approves first MASH drug	159

Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	158
Connecting research and clinical practice: training the next generation of translational scientists	158
Artificial miRNA slows Huntington’s	157
Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity	157
Assessing workforce needs for the emerging CAR-T cell therapy industry	156
Grand challenges in bioinformatics education and training	155
A language model beats alphafold2 on orphans	155
Delivering 3 billion doses of Comirnaty in 2021	155
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	154
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	152
Prediction of peptide mass spectral libraries with machine learning	152
Detection of mosaic and population-level structural variants with Sniffles2	151
A strategy to load, rethread and read protein sequences through a nanopore	150
2022 – toughing out the trough	149
Drug pipeline 4Q22 — sticking around	148
A circularly permuted CasRx platform for efficient, site-specific RNA editing	146
Chemigenetic kinase biosensors illuminate cell signaling networks	146
Biotech news from around the world	144
100,000 whole-genome sequences’ diagnostic bonus	144
Forum: Boyden and Danzl	141
Lab-made antibody stops malaria	140
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	139
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	138
Genome-edited farm animals from haploid stem cells	137
Moderna feud with NIH over COVID vaccine	135
COVID vaccine IP waiver agreed	135
Consternation follows Marks’ FDA departure	130
Engineering pattern recognition receptors facilitates plant resistance breeding	128
Enzymatic synthesis of RNA oligonucleotides	127
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	127
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	125
Microbiome-friendly phages join the campaign for better antimicrobials	125
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	124
Precision RNA base editing with engineered and endogenous effectors	121
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	117
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	117
Baby’s first genome	115
RNA stability enhancers for durable base-modified mRNA therapeutics	114
Cell type inference in cell-free nucleic acid liquid biopsy	114
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	113
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	113
Spatial host–microbiome sequencing reveals niches in the mouse gut	112
Chronic nasal congestion gets new treatment	111
Data sharing in the age of deep learning	111
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	109
The devolution of biosimilars regulations	109
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	108
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	107
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	106
Editor’s pick: Red Queen Therapeutics	106
How will generative AI disrupt data science in drug discovery?	106
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	106
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	105
Antivirals	103
Publisher Correction: Treg engineers take aim at autoimmunity	102
Base editors	102
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	102
Forum: Drug delivery roundtable with Langer and Whitehead	100
2024: research in review	100
Sensitive detection of gene transfer in a microbial community	100
An organoid model with all three pancreatic lineages resembles fetal pancreas	99
Stem cells	99
Ionis wins FDA nod for triglyceride-lowering rare disease drug	99
People	99
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	98
A genome-wide view of disordered proteins	97
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	97
CRISPR beef cattle get FDA green light	95
Thinking about a move from academia to industry? Look before you leap	94
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	94
Spotlight on cancer immunotherapies	94
Senolytics under scrutiny in the quest to slow aging	94
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	93
Next-generation patient models for colorectal cancer research	93
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	93
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	93
AI-enhanced protein design makes proteins that have never existed	92
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	92
The strength and importance of government-funded patents for approved drugs	92
The GA4GH Phenopacket schema defines a computable representation of clinical data	92

Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	91
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	90
Generation of accurate, expandable phylogenomic trees with uDance	89
Children with rare genetic diseases get CRISPR Cures center	88
Five questions with César de la Fuente	88
Septerna: making another run on GPCRs	88
Accurate somatic small variant discovery for multiple sequencing technologies with DeepSomatic	87
Author Correction: Engineering circular RNA for enhanced protein production	87
TCR cell therapies vanquish solid tumors — finally	86
Genotoxins from gut bacteria	86
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	85
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	83
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	83
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	83
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin	83
A community effort to optimize sequence-based deep learning models of gene regulation	83
How will AI affect patent disclosures?	83
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	83
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	82
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	82
Tuning plant phenotypes by precise, graded downregulation of gene expression	82
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	81
Efficient prime editing in mouse brain, liver and heart with dual AAVs	81
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	81
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	81
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	80
Starving tumors with fat	80
Fashion’s microbial dyeing machines	80
Measuring the impact of chromatin context on transcription factor binding affinities	80
People	80
2H23 biotech job picture	79
Biotech news from around the world	79
Clarifying US regulations on xenotransplantation	78
Microfluidics	78
Base editor restores CD3δ-dependent SCID mutation	78
Microbiomics	77
Discovering the crucial function of long noncoding RNAs	77
Publisher Correction: Top 20 translational researchers of 2020	76
The instant spray-on dress	76
Bridge RNAs direct programmable DNA rearrangements	76
Biotech patenting 2021	75
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	75
Startup grows egg proteins in potato fields	75
Big pharma seduced by transcription factors again. What has changed?	74
Embryonic stem cells across bird species	74
Using artificial intelligence to develop gene therapy for the lungs	73
Chikungunya vaccine approved	73
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	73
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	72
Make science disruptive again	72
Combination therapy patents: a new front in evergreening	72
Eli Lilly in $650M pact to boost muscle	70
The DxConnect Virtual Biobank connects diagnostic researchers to clinical samples	70
Capturing the diversity of protein modifications on presented tumor antigens	69
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	69
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	68
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	68
A shifting market environment and strategies fueling innovation in prostate cancer treatment	68
How to grow cement	67
Prime editing	67
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	67
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	67
Bio-leather gears up to wow fashion industry	66
Fighting fibrosis	65
Wearable ultrasound for continuous deep-tissue monitoring	65
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	65
Curated variation benchmarks for challenging medically relevant autosomal genes	65
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	64
Redefining antibody patent protection using paratope mapping and CDR-scanning	63
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	63
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	62
Challenges for developing broad-based mucosal vaccines for respiratory viruses	62
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	62
RNA interference in the era of nucleic acid therapeutics	62
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	62
Single-molecule live-cell RNA imaging with CRISPR–Csm	62
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	61
A highly photostable and bright green fluorescent protein	61
Generation of a live attenuated influenza A vaccine by proteolysis targeting	61
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	60
The challenges and promise of sweat sensing	60
Second-quarter biotech job picture	59
Public biotech in 2021 — the numbers	59
Biotech news from around the world	58
Biomedical imaging	57
Visualizing protein nanostructures in intact brain	57
Forum: Locke and Sykes	57
First in vivo base editing lowers cholesterol	57
Biotech news from around the world	57
Radiopharmaceuticals	56
The long game of tenure	56
Durable epigenetic silencing with a TALE-based editor	56
A retrotransposon for site-specific gene transfer	56
Ocean-grown bioplastics	56
Wasp gut microbes yield beer with extra tang	56