Nature Biotechnology

Article	Citations
Framework for predicting alloreactivity in hematopoietic cell transplants	1641
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	1476
The champions of drug development	1100
People	1001
Cancer Moonshot aims at liquid biopsies	704
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	624
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	557
AI’s plastic recycling	555
Fluorescence microscopy resolution increases to near-atomic level	530
Single-cell RNA-seq keeps cells alive	504
Ultra-precision medicine	471
Ebola as a case study for the patent landscape of medical countermeasures for emerging infectious diseases	469
Diffusion model expands RoseTTAFold’s power	465
Fungal architecture is a thing, and it’s eye-catching	439
Behind the graduate mental health crisis in science	415
Dreaming ideal protein structures	388
Synthetic biologics provide a path to target bHLH transcription factors	386
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	361
An optimized toolkit for prime editing	357
Engineered serum markers for non-invasive monitoring of gene expression in the brain	355
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	326
Negative innovation: when patents are bad for patients	323
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	313
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	303
Generative models for protein structures and sequences	299

Denmark invests in mucosal vaccines	296
Wireless microelectronic fibers to discover gut and brain pathways	292
People	281
Bite-sized solutions	280
Engineering innate immune cells for cancer immunotherapy	274
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	274
Cellular shuttles put mislocalized proteins in their right place	266
Variant to function mapping at single-cell resolution through network propagation	263
Droplet-based transcriptome profiling of individual synapses	262
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	258
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	251
Chem-map profiles drug binding to chromatin in cells	250
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	249
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	249
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	247
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	242
An oncolytic virus–T cell chimera for cancer immunotherapy	237
Democratizing knowledge representation with BioCypher	233
The lysosomal degraders	230
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	223
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	218
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	216
A small and highly sensitive red/far-red optogenetic switch for applications in mammals	210
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	208
The world’s first CRISPR therapy is approved: who will receive it?	205
3Q22 — more malaise in the markets	203
Biotech news from around the world	195
Light-sheet microscopy at high resolution	190
HSV-1’s contribution as a vector for gene therapy	188
Hallucinating functional protein sequences	185
The need for need-finding in medical education	184
Author Correction: The cost of drug patent expiration date errors	183
Nanotechnology offers alternative ways to fight COVID-19 pandemic with antivirals	178
Live cell imaging of signaling networks using a conventional microscope	171
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	169
Delix Therapeutics : psychedelics without the trip	169
COVID-19 testing turns to T cells	167
AI predicts cancer relapse from slides	165
Towards the non-invasive imaging of brain networks and functions at high resolution	161
Biotech news from around the world	160
People	159
Adrestia Therapeutics — Gene networks to the rescue	158
Slaughter-free meat hits the grocery shelf	158
Cancer diagnostics	157
Assessing workforce needs for the emerging CAR-T cell therapy industry	153
Next-generation psychedelics: should new agents skip the trip?	152
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	151
Extended-representation bisulfite sequencing of gene regulatory elements in multiplexed samples and single cells	151
FDA approves first MASH drug	150
Grand challenges in bioinformatics education and training	148

A language model beats alphafold2 on orphans	148
Prediction of peptide mass spectral libraries with machine learning	146
Delivering 3 billion doses of Comirnaty in 2021	145
Who will oversee the ethical limits of human embryo research?	145
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	144
Decoding gene regulation with CRISPR perturbations	142
Gains in early-onset dementia with progranulin open new paths for drug discovery	142
Orna Therapeutics: circular logic	141
Precise DNA cleavage using CRISPR-SpRYgests	141
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	137
Detection of mosaic and population-level structural variants with Sniffles2	135
Mapping single-cell data to reference atlases by transfer learning	134
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	134
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	134
Engineered pegRNAs improve prime editing efficiency	134
2022 – toughing out the trough	133
Drug pipeline 4Q22 — sticking around	130
Top 20 translational researchers of 2020	129
Biotech news from around the world	129
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	128
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	127
Microbiome-friendly phages join the campaign for better antimicrobials	127
How will generative AI disrupt data science in drug discovery?	127
Precision RNA base editing with engineered and endogenous effectors	126
Spatial host–microbiome sequencing reveals niches in the mouse gut	125
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	122
Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin	122
Data sharing in the age of deep learning	122
Microbiomes	121
A carte blanche approval in Alzheimer’s	121
100,000 whole-genome sequences’ diagnostic bonus	121
Forum: Boyden and Danzl	120
Biotech news from around the world	120
Lab-made antibody stops malaria	120
Synthetic virology: the experts speak	117
Moderna feud with NIH over COVID vaccine	117
COVID vaccine IP waiver agreed	117
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	115
Enzymatic synthesis of RNA oligonucleotides	115
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	113
Baby’s first genome	113
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	112
The devolution of biosimilars regulations	110
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	110
A circularly permuted CasRx platform for efficient, site-specific RNA editing	110
Chemigenetic kinase biosensors illuminate cell signaling networks	109
A strategy to load, rethread and read protein sequences through a nanopore	109
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	108
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	108
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	106
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	105
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	105
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	104
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	104
Base editors	103
Next-generation patient models for colorectal cancer research	102
Antivirals	102
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	101
Publisher Correction: Treg engineers take aim at autoimmunity	101
People	99
Forum: Drug delivery roundtable with Langer and Whitehead	96
CRISPR beef cattle get FDA green light	96
An organoid model with all three pancreatic lineages resembles fetal pancreas	96
How will AI affect patent disclosures?	96
Ionis wins FDA nod for triglyceride-lowering rare disease drug	96
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	95
Five questions with César de la Fuente	95
Publisher Correction: Questions swirl around failures of disease-modifying Huntington’s drugs	93
2024: research in review	93
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	93
Stem cells	93
Publisher Correction: Send in the senolytics	91
People	91
Sensitive detection of gene transfer in a microbial community	90
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	90
Author Correction: Engineering circular RNA for enhanced protein production	90
A genome-wide view of disordered proteins	90
Generation of accurate, expandable phylogenomic trees with uDance	90
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	89
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	88

Septerna: making another run on GPCRs	88
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	88
Purification of multiplex oligonucleotide libraries by synthesis and selection	88
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	88
TCR cell therapies vanquish solid tumors — finally	86
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	86
Spotlight on cancer immunotherapies	85
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	84
A community effort to optimize sequence-based deep learning models of gene regulation	83
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	82
Tuning plant phenotypes by precise, graded downregulation of gene expression	81
AI-enhanced protein design makes proteins that have never existed	81
Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis	81
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	80
Efficient prime editing in mouse brain, liver and heart with dual AAVs	80
Genotoxins from gut bacteria	80
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	79
Fully implantable and bioresorbable cardiac pacemakers without leads or batteries	79
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	78
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	77
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	77
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	77
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	77
The GA4GH Phenopacket schema defines a computable representation of clinical data	77
2H23 biotech job picture	76
Clarifying US regulations on xenotransplantation	76
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	75
Starving tumors with fat	75
Measuring the impact of chromatin context on transcription factor binding affinities	74
Fashion’s microbial dyeing machines	74
People	74
Biotech news from around the world	74
Base editor restores CD3δ-dependent SCID mutation	73
Microfluidics	73
Microbiomics	72
The instant spray-on dress	71
Bridge RNAs direct programmable DNA rearrangements	71
The viral arms race	69
Publisher Correction: Top 20 translational researchers of 2020	69
Redefining antibody patent protection using paratope mapping and CDR-scanning	69
Startup grows egg proteins in potato fields	69
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	69
Biotech patenting 2021	69
Challenges for developing broad-based mucosal vaccines for respiratory viruses	68
Fighting fibrosis	68
Discovering the crucial function of long noncoding RNAs	68
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	67
Circular RNAs sequenced at last	67
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	67
Prime editing	67
How to grow cement	67
A shifting market environment and strategies fueling innovation in prostate cancer treatment	67
Using artificial intelligence to develop gene therapy for the lungs	67
The DxConnect Virtual Biobank connects diagnostic researchers to clinical samples	67
Curated variation benchmarks for challenging medically relevant autosomal genes	66
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	66
Make science disruptive again	66
Generation of a live attenuated influenza A vaccine by proteolysis targeting	66
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	65
Capturing the diversity of protein modifications on presented tumor antigens	65
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	65
Chikungunya vaccine approved	65
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	64
Combination therapy patents: a new front in evergreening	64
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	64
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	64
Single-molecule live-cell RNA imaging with CRISPR–Csm	64
A highly photostable and bright green fluorescent protein	64
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	63
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	63
Bioorthogonal information storage in l-DNA with a high-fidelity mirror-image Pfu DNA polymerase	63
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	63
The challenges and promise of sweat sensing	62
Wearable ultrasound for continuous deep-tissue monitoring	62
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	61
Visualizing protein nanostructures in intact brain	61
Bio-leather gears up to wow fashion industry	61
RNA interference in the era of nucleic acid therapeutics	61
Biotech news from around the world	60
Baby genomics: newborn sequencing starts to fulfill its promise	59
Biotech news from around the world	59
People	59
Fluorescence imaging of RNA and protein in deep tissue	59
Public biotech in 2021 — the numbers	59
People	58
Increasing the success potential of promising biotech companies	58
Second-quarter biotech job picture	58
Innovators want pills to treat sickle cell disease. Can they match gene therapy?	57
Forum: Locke and Sykes	57
Biomedical imaging	57
Italy tests first gene-edited vines for winemaking	57
For hemophilia and thalassemia, a new era of ‘one-and-done’ gene therapies has arrived	57
First in vivo base editing lowers cholesterol	56
Wireless multisite physiological sensor with clinical-grade accuracy	56
Insulin — the new battleground for drug pricing	55