Nature Biotechnology

Article	Citations
Single-cell RNA-seq keeps cells alive	5238
Synthetic biologics provide a path to target bHLH transcription factors	2462
Denmark invests in mucosal vaccines	1367
Wireless microelectronic fibers to discover gut and brain pathways	1140
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	1043
Framework for predicting alloreactivity in hematopoietic cell transplants	918
Bite-sized solutions	800
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	633
Human periportal liver assembloids	566
Cancer Moonshot aims at liquid biopsies	514
People	514
Cellular shuttles put mislocalized proteins in their right place	477
Diffusion model expands RoseTTAFold’s power	440
Author Correction: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew	428
Establishing a commercial solution for extremely rare genetic diseases	411
The lysosomal degraders	399
Fluorescence microscopy resolution increases to near-atomic level	383
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	374
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	369
Biopharma dives into tumor-seeking radioactive drugs	356
Synthetic biology’s uncertain regulatory future in the wake of Loper Bright Enterprises v. Raimondo	344
Fungal architecture is a thing, and it’s eye-catching	319
Single-cell spatial pharmacobiology for imaging antibody-based therapies in solid tumors	313
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	285
Fresh from the biotech pipeline: FDA turmoil overshadows 2025 approvals	284

Programming biology: next-gen AI firms raise billions to design better medicines	275
Spatial CRISPR screens map total RNA in tissue	269
Biotech news from around the world	267
Orchestration of secondary metabolite production in Streptomyces species	266
An optimized toolkit for prime editing	258
Chem-map profiles drug binding to chromatin in cells	251
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	250
Democratizing knowledge representation with BioCypher	245
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	245
Generative models for protein structures and sequences	241
An oncolytic virus–T cell chimera for cancer immunotherapy	239
Dual SORT LNPs for multi-organ base editing	236
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	232
The world’s first CRISPR therapy is approved: who will receive it?	219
Agentic AI and the rise of in silico team science in biomedical research	214
Engineered serum markers for non-invasive monitoring of gene expression in the brain	212
Droplet-based transcriptome profiling of individual synapses	211
Behind the graduate mental health crisis in science	210
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	209
Programmable promoter editing for precise control of transgene expression	208
Design of optimized epigenetic regulators for durable gene silencing with application to PCSK9 in nonhuman primates	207
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	205
A spatial view of tumor neoantigen recognition	204
Engineering innate immune cells for cancer immunotherapy	203
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	201
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	199
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	198
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	197
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	194
AI predicts cancer relapse from slides	193
Biotech news from around the world	189
3Q22 — more malaise in the markets	188
The need for need-finding in medical education	188
Biotech news from around the world	183
People	180
Decoding gene regulation with CRISPR perturbations	178
Delix Therapeutics : psychedelics without the trip	177
Sequence Display enables large-scale sequence–activity datasets for rapid protein evolution	174
Computationally designed enzymes show potent catalytic activity	173
Adrestia Therapeutics — Gene networks to the rescue	170
Connecting research and clinical practice: training the next generation of translational scientists	168
Mapping the patent landscape of cancer diagnostics	164
GLP-1s surprise failure in Alzheimer’s	163
Next-generation psychedelics: should new agents skip the trip?	163
In vivo base editing reverses a neurodevelopmental disorder	156
Lessons from biotech’s unscientific evolution	156
Author Correction: The cost of drug patent expiration date errors	155
David Baltimore (1938–2025)	155
Hallucinating functional protein sequences	154
Escaping analysis paralysis in medical training: from FRAZZLE to CALM	148

Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	147
Precise DNA cleavage using CRISPR-SpRYgests	146
Artificial miRNA slows Huntington’s	144
Gains in early-onset dementia with progranulin open new paths for drug discovery	142
HSV-1’s contribution as a vector for gene therapy	141
Slaughter-free meat hits the grocery shelf	140
Live cell imaging of signaling networks using a conventional microscope	137
A retargeted recombinase for precise insertion of large DNA	135
Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity	134
FDA approves first MASH drug	133
Orna Therapeutics: circular logic	131
Reply to ‘Methodological concerns and a lack of evidence for reforming regulatory exclusivities for pharmaceuticals’	128
Improving metagenome binning by integrating intrinsic features and taxonomy	127
Grand challenges in bioinformatics education and training	127
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	126
Considerations for the future of in vitro gametogenesis in fertility care	125
Prediction of peptide mass spectral libraries with machine learning	125
Editor’s pick: Liberate Bio	125
A language model beats alphafold2 on orphans	124
Delivering 3 billion doses of Comirnaty in 2021	121
The promises and challenges of neoantigen cancer vaccines	121
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	121
Detection of mosaic and population-level structural variants with Sniffles2	119
Building synthetic chromosomes one yeast at a time: insights from Sc2.0	119
2022 – toughing out the trough	119
A strategy to load, rethread and read protein sequences through a nanopore	119
Drug pipeline 4Q22 — sticking around	118
Biotech news from around the world	118
Lab-made antibody stops malaria	117
Forum: Boyden and Danzl	117
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	117
Unpaired data as a first-order challenge in single-cell and spatial proteomics	115
The global patent landscape of optogenetics: current advances, challenges and opportunities	114
The devolution of biosimilars regulations	114
Enzymatic synthesis of RNA oligonucleotides	113
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	112
An update on patent issues regarding antibody–drug conjugates	111
Genome-edited farm animals from haploid stem cells	111
How will generative AI disrupt data science in drug discovery?	111
Engineering pattern recognition receptors facilitates plant resistance breeding	109
Editor’s pick: Red Queen Therapeutics	109
Chronic nasal congestion gets new treatment	109
Consternation follows Marks’ FDA departure	107
Cell type inference in cell-free nucleic acid liquid biopsy	106
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	105
Chemigenetic kinase biosensors illuminate cell signaling networks	105
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	104
Microbiome-friendly phages join the campaign for better antimicrobials	104
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	103
Quantifying endosomal escape in vivo to guide lipid nanoparticle design	102
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	102
A circularly permuted CasRx platform for efficient, site-specific RNA editing	100
Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments	100
Data sharing in the age of deep learning	100
RNA stability enhancers for durable base-modified mRNA therapeutics	100
Precision RNA base editing with engineered and endogenous effectors	100
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	99
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	99
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	98
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	98
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	97
Spatial host–microbiome sequencing reveals niches in the mouse gut	97
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	97
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	96
Stem cells	95
Base editors	95
People	93
Ionis wins FDA nod for triglyceride-lowering rare disease drug	92
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	91
An organoid model with all three pancreatic lineages resembles fetal pancreas	91
Cross-species snakebite antivenom	90
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	89
Thinking about a move from academia to industry? Look before you leap	89
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	88
Children with rare genetic diseases get CRISPR Cures center	88
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	88
Five questions with César de la Fuente	88
Next-generation patient models for colorectal cancer research	87
Septerna: making another run on GPCRs	87
Gene drives tested against real-world malaria diversity	87

Genotoxins from gut bacteria	86
Sensitive detection of gene transfer in a microbial community	85
Spotlight on cancer immunotherapies	85
How will AI affect patent disclosures?	84
The strength and importance of government-funded patents for approved drugs	84
A genome-wide view of disordered proteins	84
Author Correction: Engineering circular RNA for enhanced protein production	82
Generation of accurate, expandable phylogenomic trees with uDance	82
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	80
Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates	80
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin	80
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	80
AI-enhanced protein design makes proteins that have never existed	79
TCR cell therapies vanquish solid tumors — finally	79
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	79
Senolytics under scrutiny in the quest to slow aging	78
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	78
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	78
Tuning plant phenotypes by precise, graded downregulation of gene expression	78
2024: research in review	77
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	77
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	76
Efficient prime editing in mouse brain, liver and heart with dual AAVs	76
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	75
A community effort to optimize sequence-based deep learning models of gene regulation	75
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	74
Accurate somatic small variant discovery for multiple sequencing technologies with DeepSomatic	73
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	72
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	71
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	71
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	71
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	71
Measuring the impact of chromatin context on transcription factor binding affinities	70
People	70
2H23 biotech job picture	69
Base editor restores CD3δ-dependent SCID mutation	68
Microfluidics	67
Discovering the crucial function of long noncoding RNAs	67
Microbiomics	67
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	65
Bridge RNAs direct programmable DNA rearrangements	65
Startup grows egg proteins in potato fields	65
Big pharma seduced by transcription factors again. What has changed?	64
Embryonic stem cells across bird species	64
Using artificial intelligence to develop gene therapy for the lungs	64
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	63
Capturing the diversity of protein modifications on presented tumor antigens	63
Make science disruptive again	63
Newcomer anti-IL-33 makes strides in COPD	63
Culturing microbiome therapeutics with big data	63
Eli Lilly in $650M pact to boost muscle	62
A shifting market environment and strategies fueling innovation in prostate cancer treatment	62
Gabriele et al. reply	62
AAV-delivered UGA suppressor tRNA for disease-agnostic in vivo gene therapy	62
Starving tumors with fat	62
Fighting fibrosis	61
Prime editing	61
The instant spray-on dress	60
Programmable control of bacterial operons with a single Cas13 RNA effector	60
Chikungunya vaccine approved	59
Artificial intelligence in drug discovery	59
Redefining antibody patent protection using paratope mapping and CDR-scanning	59
Light-powered metabolism in the mammalian eye	59
Mapping the spatial landscape of extracellular vesicles in tissues with Spatial-EV-seq	58
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	58
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	58
Precise, minimally evolved adenine base editors generated through mutation reversion analysis	58
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	57
Wearable ultrasound for continuous deep-tissue monitoring	57
RNA interference in the era of nucleic acid therapeutics	57
Predicting small molecule–RNA interactions without RNA tertiary structures	56
The global patent landscape of bispecific antibodies: technological innovations and therapeutic applications	56
Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq	56
Challenges for developing broad-based mucosal vaccines for respiratory viruses	56
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	55
Single-molecule live-cell RNA imaging with CRISPR–Csm	55
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	55
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	55
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	54
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	54
The challenges and promise of sweat sensing	54
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	54
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	54
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	54
Second-quarter biotech job picture	53
Public biotech in 2021 — the numbers	53
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	53
Visualizing protein nanostructures in intact brain	52
Biotech news from around the world	52
Biotech news from around the world	51
A retrotransposon for site-specific gene transfer	50
Wasp gut microbes yield beer with extra tang	50
Biomedical imaging	50
The long game of tenure	50
Forum: Locke and Sykes	50