Nature Biotechnology

Article	Citations
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	2244
The champions of drug development	1901
People	1352
Cancer Moonshot aims at liquid biopsies	1260
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	820
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	725
AI’s plastic recycling	674
Fluorescence microscopy resolution increases to near-atomic level	653
Single-cell RNA-seq keeps cells alive	640
Fungal architecture is a thing, and it’s eye-catching	570
Dreaming ideal protein structures	562
Synthetic biologics provide a path to target bHLH transcription factors	525
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	521
An optimized toolkit for prime editing	512
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	503
Drug pipeline 1Q22 — a raft of new modalities … and clinical blowups	403
Framework for predicting alloreactivity in hematopoietic cell transplants	387
Denmark invests in mucosal vaccines	379
Wireless microelectronic fibers to discover gut and brain pathways	364
People	356
Bite-sized solutions	354
Diffusion model expands RoseTTAFold’s power	348
Engineered serum markers for non-invasive monitoring of gene expression in the brain	340
Dual SORT LNPs for multi-organ base editing	337
Cellular shuttles put mislocalized proteins in their right place	321

Biopharma dives into tumor-seeking radioactive drugs	318
The lysosomal degraders	316
Variant to function mapping at single-cell resolution through network propagation	310
The world’s first CRISPR therapy is approved: who will receive it?	289
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	287
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	286
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	271
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	264
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	263
Generative models for protein structures and sequences	263
Behind the graduate mental health crisis in science	262
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	258
Democratizing knowledge representation with BioCypher	255
A small and highly sensitive red/far-red optogenetic switch for applications in mammals	251
An oncolytic virus–T cell chimera for cancer immunotherapy	248
Engineering innate immune cells for cancer immunotherapy	234
Droplet-based transcriptome profiling of individual synapses	220
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	218
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	208
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	208
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	198
Chem-map profiles drug binding to chromatin in cells	198
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	197
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	193
3Q22 — more malaise in the markets	191
Biotech news from around the world	189
Light-sheet microscopy at high resolution	188
The need for need-finding in medical education	187
Author Correction: The cost of drug patent expiration date errors	182
Live cell imaging of signaling networks using a conventional microscope	180
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	180
Delix Therapeutics : psychedelics without the trip	178
AI predicts cancer relapse from slides	177
Biotech news from around the world	176
Towards the non-invasive imaging of brain networks and functions at high resolution	176
People	171
Next-generation psychedelics: should new agents skip the trip?	170
Cancer diagnostics	170
Who will oversee the ethical limits of human embryo research?	168
Hallucinating functional protein sequences	167
Nanotechnology offers alternative ways to fight COVID-19 pandemic with antivirals	165
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	164
Precise DNA cleavage using CRISPR-SpRYgests	164
Gains in early-onset dementia with progranulin open new paths for drug discovery	164
Adrestia Therapeutics — Gene networks to the rescue	163
Decoding gene regulation with CRISPR perturbations	163
Delivering 3 billion doses of Comirnaty in 2021	162
Slaughter-free meat hits the grocery shelf	160
A language model beats alphafold2 on orphans	159
Computationally designed enzymes show potent catalytic activity	156

FDA approves first MASH drug	155
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	153
HSV-1’s contribution as a vector for gene therapy	151
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	151
Orna Therapeutics: circular logic	150
Prediction of peptide mass spectral libraries with machine learning	149
Assessing workforce needs for the emerging CAR-T cell therapy industry	148
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	148
Grand challenges in bioinformatics education and training	146
Detection of mosaic and population-level structural variants with Sniffles2	146
Engineered pegRNAs improve prime editing efficiency	145
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	145
Biotech news from around the world	144
2022 – toughing out the trough	144
Drug pipeline 4Q22 — sticking around	144
A strategy to load, rethread and read protein sequences through a nanopore	143
Top 20 translational researchers of 2020	143
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	142
Chemigenetic kinase biosensors illuminate cell signaling networks	142
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	141
100,000 whole-genome sequences’ diagnostic bonus	141
Data sharing in the age of deep learning	141
Biotech news from around the world	139
Forum: Boyden and Danzl	136
Lab-made antibody stops malaria	136
Synthetic virology: the experts speak	135
COVID vaccine IP waiver agreed	133
Moderna feud with NIH over COVID vaccine	133
The devolution of biosimilars regulations	132
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	132
Microbiome-friendly phages join the campaign for better antimicrobials	129
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	127
Consternation follows Marks’ FDA departure	127
A circularly permuted CasRx platform for efficient, site-specific RNA editing	126
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	126
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	125
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	124
Precision RNA base editing with engineered and endogenous effectors	123
How will generative AI disrupt data science in drug discovery?	121
Enzymatic synthesis of RNA oligonucleotides	121
Spatial host–microbiome sequencing reveals niches in the mouse gut	120
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	118
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	118
Baby’s first genome	116
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	115
Chromatin Velocity reveals epigenetic dynamics by single-cell profiling of heterochromatin and euchromatin	114
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	114
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	111
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	111
CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information	110
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	109
Antivirals	108
Base editors	108
Publisher Correction: Treg engineers take aim at autoimmunity	107
Relieving patent-eligibility barriers in biotech with a preparation or treatment method	106
Forum: Drug delivery roundtable with Langer and Whitehead	105
How will AI affect patent disclosures?	105
Ionis wins FDA nod for triglyceride-lowering rare disease drug	105
An organoid model with all three pancreatic lineages resembles fetal pancreas	103
2024: research in review	103
Five questions with César de la Fuente	103
Stem cells	102
People	102
Sensitive detection of gene transfer in a microbial community	101
Publisher Correction: Send in the senolytics	101
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	99
Author Correction: Engineering circular RNA for enhanced protein production	99
A genome-wide view of disordered proteins	97
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	95
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	94
Next-generation patient models for colorectal cancer research	94
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	93
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	93
Genotoxins from gut bacteria	93
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	93
Thinking about a move from academia to industry? Look before you leap	93
Septerna: making another run on GPCRs	92
CRISPR beef cattle get FDA green light	91
Spotlight on cancer immunotherapies	91
Senolytics under scrutiny in the quest to slow aging	90

TCR cell therapies vanquish solid tumors — finally	89
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	88
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	88
Generation of accurate, expandable phylogenomic trees with uDance	87
A community effort to optimize sequence-based deep learning models of gene regulation	87
The strength and importance of government-funded patents for approved drugs	87
Children with rare genetic diseases get CRISPR Cures center	87
Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors	86
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	86
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	85
Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis	85
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	85
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	85
Accelerated identification of disease-causing variants with ultra-rapid nanopore genome sequencing	84
The GA4GH Phenopacket schema defines a computable representation of clinical data	84
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	83
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	83
Tuning plant phenotypes by precise, graded downregulation of gene expression	83
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	82
Efficient prime editing in mouse brain, liver and heart with dual AAVs	82
AI-enhanced protein design makes proteins that have never existed	82
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	81
Clarifying US regulations on xenotransplantation	80
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	80
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	80
2H23 biotech job picture	80
Starving tumors with fat	79
People	79
Microfluidics	78
Biotech news from around the world	78
Base editor restores CD3δ-dependent SCID mutation	78
Measuring the impact of chromatin context on transcription factor binding affinities	78
Microbiomics	78
Fashion’s microbial dyeing machines	78
Bridge RNAs direct programmable DNA rearrangements	77
The instant spray-on dress	77
Redefining antibody patent protection using paratope mapping and CDR-scanning	76
Biotech patenting 2021	76
Startup grows egg proteins in potato fields	75
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	74
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	74
Fighting fibrosis	74
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	74
Discovering the crucial function of long noncoding RNAs	74
Chikungunya vaccine approved	74
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	74
Publisher Correction: Top 20 translational researchers of 2020	74
A shifting market environment and strategies fueling innovation in prostate cancer treatment	73
Make science disruptive again	73
Bio-leather gears up to wow fashion industry	72
Prime editing	72
How to grow cement	71
Wearable ultrasound for continuous deep-tissue monitoring	71
Capturing the diversity of protein modifications on presented tumor antigens	71
Single-molecule live-cell RNA imaging with CRISPR–Csm	71
Using artificial intelligence to develop gene therapy for the lungs	71
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	71
Eli Lilly in $650M pact to boost muscle	70
Combination therapy patents: a new front in evergreening	70
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	70
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	70
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	69
Challenges for developing broad-based mucosal vaccines for respiratory viruses	69
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	68
The DxConnect Virtual Biobank connects diagnostic researchers to clinical samples	68
Scalable single-cell RNA sequencing from full transcripts with Smart-seq3xpress	68
A highly photostable and bright green fluorescent protein	67
The challenges and promise of sweat sensing	66
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	66
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	66
RNA interference in the era of nucleic acid therapeutics	66
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	65
Curated variation benchmarks for challenging medically relevant autosomal genes	64
Generation of a live attenuated influenza A vaccine by proteolysis targeting	64
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	64
Biotech news from around the world	63
Visualizing protein nanostructures in intact brain	63
People	63
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	63
Biotech news from around the world	63
Public biotech in 2021 — the numbers	62
Second-quarter biotech job picture	62
A retrotransposon for site-specific gene transfer	61
Forum: Locke and Sykes	61
Biomedical imaging	61
Insulin — the new battleground for drug pricing	61
The long game of tenure	61
First in vivo base editing lowers cholesterol	61
Sizing up beta cells made from stem cells	59
Author Correction: m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome	58
A multi-kingdom genetic barcoding system for precise clone isolation	58
Multistate and functional protein design using RoseTTAFold sequence space diffusion	58
Wasp gut microbes yield beer with extra tang	58
Simultaneous single-cell analysis of 5mC and 5hmC with SIMPLE-seq	58
The emerging landscape of engineered bacteria cancer therapies	57