Nature Biotechnology

Article	Citations
Writing cellular history in protein chains	1365
Forum: Alexandrov and Le	879
Federation Bio: from single strains to a purely synthetic microbiome	801
The prevalence of drug patent term extensions in the United States, 2000–2018	653
People	580
Forum: Optogenetics roundtable with Deisseroth and Gradinaru	572
Multiplexed mapping of chromatin features at single-cell resolution	518
First Rounders: Jay Keasling	438
Synthetic GPCRs for controlling cellular responses	434
Base editors	403
Forum: CRISPR screening roundtable with Stegmaier and Doench	400
People	374
Biotech news from around the world	360
Bilateral China–Brazil ties over agbiotech	349
Protein structure prediction with in-cell photo-crosslinking mass spectrometry and deep learning	333
Droplet-based transcriptome profiling of individual synapses	328
Expanding access to CAR T cell therapies through local manufacturing	308
Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro	307
Transplantation of a human liver following 3 days of ex situ normothermic preservation	306
Bluebird’s CALD gene therapy poised for approval	293
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	292
Biotech news from around the world	290
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	275
Bat biotech takes flight	271
Biotech news from around the world	256

Diffusion model expands RoseTTAFold’s power	248
Fungal architecture is a thing, and it’s eye-catching	244
Engineered Cas13 variants with minimal collateral RNA targeting	243
People	238
Ultra-precision medicine	236
Linking genetic variants to cellular function and disease	236
Predicting disease variants using biodiversity and machine learning	234
First Rounders: Lita Nelsen	225
Photys Therapeutics: customizing phosphorylation via molecular matchmaking	225
The champions of drug development	222
Putting CRISPR into African hands to future-proof crops	221
Women build strength in numbers	219
Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice	215
Dreaming ideal protein structures	215
Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH	214
Synthetic biologics provide a path to target bHLH transcription factors	212
Patents benefit patients and patent reform would spur diagnostic and therapeutic development	209
Increase vigilance against cyberattacks	209
Biotech news from around the world	205
Vector BioPharma: resurrecting adenoviral gene delivery	200
Multiplex-GAM detects complex chromatin interactions	192
Fluorescence microscopy resolution increases to near-atomic level	185
Eight diseases for gene therapy accelerator	181
People	179
Do patent applications and Cooperative Research and Development Agreements between the National Cancer Institute and industry serve the public interest?	178
Editor’s pick: Tenpoint Therapeutics	176
Wearable devices	176
Decoding the building blocks of cellular processes from single-cell transcriptomics data	174
Supervised discovery of interpretable gene programs from single-cell data	168
Biotech news from around the world	166
Single-cell m6A mapping in vivo using picoMeRIP–seq	165
Protein design: the experts speak	158
Survival of the fittest glia	158
Radical solutions	157
Big pharma craves slice of AI-based RNA drug discovery	151
Monitoring protein proximity with fluorogens	151
Novel recombinases for large DNA insertions	150
Nanopore sequencing	148
Sparks of function by de novo protein design	147
Base editing marches on the clinic	147
Chemical and topological design of multicapped mRNA and capped circular RNA to augment translation	145
Merck enlists trispecific killers	142
An improved organoid model of the human cerebellum	142
The digital and analog worlds of protein engineering	142
Mapping mRNA modifications for functional studies	140
Dendritic cell revival	139
Cancer Moonshot aims at liquid biopsies	137
Single-cell multi-omics allows functional characterization of structural variants	136
AI’s plastic recycling	134
Evolutionary gambit to defeat drug resistance in cancer	133

FDA okays first cardiac myosin inhibitor	133
Biotech news from around the world	132
Citizen chickpeas	131
Vision–language AI assistance in human pathology	130
Single-cell RNA-seq keeps cells alive	125
100,000 genomes — in Africa, for Africa	124
Biotech news from around the world	124
People	120
Has the PTAB made a difference in drug settlements and generic entry?	119
In vivo CRISPR agent cuts HAE attacks 95%	119
Single-molecule peptide sequencing on semiconductor chips	118
Supercharging T cell therapy with cancer mutations	118
Exploring tRNAs and their modifications and crosstalk using Nano-tRNAseq	117
Around the world in a month	117
Improving combination drug trials using ‘definitive screening designs’	117
Lentiviral vector cleared of causing blood cancer	116
High-throughput retrieval of target sequences from complex clone libraries using CRISPRi	116
Editor’s pick: Switch Therapeutics	113
Five questions with Weixin Tang	113
T cells	112
Cellular shuttles put mislocalized proteins in their right place	110
Accessible training for academic clinicians and researchers to address the need for academic entrepreneurs	109
An antigen discovery pipeline integrates multi-omics data and informs immunotherapy	108
Uncovering the global RNA virome	108
One-step nanoscale expansion microscopy reveals individual protein shapes	108
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	108
Government targets, end-of-year patenting rush and innovative performance in China	107
Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	106
Industry moves on 3Rs	105
People	104
Organoids and organs on a chip	104
Maintaining ‘standards’ for biosimilar monoclonal antibodies	103
Biological activity-based modeling identifies antiviral leads against SARS-CoV-2	103
Spatial characterization of single tumor cells by proteomics	103
Denmark invests in mucosal vaccines	102
Mining extinct proteomes for antimicrobial peptides	101
Contracts and intellectual property rights in translational R&D: furthering safeguards in the public interest	101
Precise intraoperative brain tumor classification	100
Detecting somatic mutations in single-cell data sets	100
Startups probe hidden viruses in the ‘dark genome’ to treat disease	99
A bacterial protease depletes c-MYC and increases survival in mouse models of bladder and colon cancer	97
CRISPR-free, strand-selective mitochondrial DNA base editing using a nickase	95
Capturing and modeling cellular niches from dissociated single-cell and spatial data	95
Wireless microelectronic fibers to discover gut and brain pathways	94
AI for drug discovery is booming, but who owns the patents?	94
Identification of antimicrobial peptides from the human gut microbiome using deep learning	94
Chroma Medicine and Tune Therapeutics: Two companies take up epigenome editing	93
CRISPR-edited plants by grafting	93
Fludarabine increases nuclease-free AAV- and CRISPR/Cas9-mediated homologous recombination in mice	93
CRISPR therapies march into clinic, but genotoxicity concerns linger	92
Club-goers take first bites of lab-made chicken	91
Startup tackles oncogene-rich ecDNA	90
RNA drugs lower lipoprotein(a) and genetically driven cholesterol	89
NIS-Seq enables cell-type-agnostic optical perturbation screening	89
Inferring cell–cell communication at single-cell resolution	89
CRISPR-StAR enables high-resolution genetic screening in complex in vivo models	88
Deletion and replacement of long genomic sequences using prime editing	87
Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics	86
The lysosomal degraders	86
Variant to function mapping at single-cell resolution through network propagation	86
Robust decomposition of cell type mixtures in spatial transcriptomics	86
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	85
Real-time monitoring of tumor-homing bacteria and tumor cells in vivo using ultrasound	85
Imaging glycosylated RNAs at the subcellular scale	85
The community of the DAO	84
After obesity drugs’ success, companies rush to preserve skeletal muscle	83
First herpesvirus gene therapy	82
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	82
The next giant step for microbes	82
Drug startups coalesce around condensates	81
Whole-body cellular mapping in mouse using standard IgG antibodies	81
CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA	80
Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells	80
Evaluating the analytical validity of circulating tumor DNA sequencing assays for precision oncology	78
Fast and accurate metagenotyping of the human gut microbiome with GT-Pro	77
A developmental route to hematopoietic stem cells	76
A proteome-wide atlas of drug mechanism of action	74
Improving the predictive power of mouse models	73
Trans-nuclease activity of Cas9 activated by DNA or RNA target binding	73
Spanish tortillas go vegan	72

HIV drug 100% protective	72
A base editor modifies bacterial genes in the guts of living mice	72
Engineered CRISPR-Cas12a for higher-order combinatorial chromatin perturbations	70
Personalized medicine is having its day	70
Mapping the patent landscape of medical machine learning	70
The rise of patient avatars in precision oncology	70
Framework for predicting alloreactivity in hematopoietic cell transplants	70
DNA writing technologies moving toward synthetic genomes	69
People	69
Virus-like elements	69
Behind the graduate mental health crisis in science	68
A multiplex implantable microdevice assay identifies synergistic combinations of cancer immunotherapies and conventional drugs	68
Recoded gene circuits for multiplexed genetic code expansion	67
Strand-selective base editing of human mitochondrial DNA using mitoBEs	67
European patent protection for medical uses of known products and drug repurposing	66
Generation of programmable splicing factors using RNA-binding proteins that activate exon inclusion	66
Rapid-response manufacturing of adenovirus-vectored vaccines	66
Long-term intravital subcellular imaging with confocal scanning light-field microscopy	65
Genentech, Sangamo ink Alzheimer’s deal	65
High-efficiency prime editing with optimized, paired pegRNAs in plants	65
People	65
Solid-phase capture and profiling of open chromatin by spatial ATAC	65
Engineered serum markers for non-invasive monitoring of gene expression in the brain	64
Chem-map profiles drug binding to chromatin in cells	64
Large language models generate functional protein sequences across diverse families	64
Wireless, closed-loop, smart bandage with integrated sensors and stimulators for advanced wound care and accelerated healing	63
High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs	63
Bioengineered corneal tissue for minimally invasive vision restoration in advanced keratoconus in two clinical cohorts	63
A microneedle vaccine printer for thermostable COVID-19 mRNA vaccines	62
Unprecedented blood biomarker enables ALS drug approval	61
A knowledge graph to interpret clinical proteomics data	61
Genotoxic effects of base and prime editing in human hematopoietic stem cells	61
The global patent landscape of artificial intelligence applications for cancer	60
gutSMASH predicts specialized primary metabolic pathways from the human gut microbiota	60
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	60
The world’s first CRISPR therapy is approved: who will receive it?	60
Enhanced cancer detection from cell-free DNA	60
A unified haplotype-based method for accurate and comprehensive variant calling	59
From air to your plate: tech startups making food from atmospheric CO2	59
Transcriptome-wide profiling and quantification of N6-methyladenosine by enzyme-assisted adenosine deamination	59
Spatial mapping of the total transcriptome by in situ polyadenylation	59
scChIX-seq infers dynamic relationships between histone modifications in single cells	58
Designing proteins with language models	58
Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing	58
NINDS launches network to develop treatments for ultra-rare neurological diseases	58
Machine learning in drug discovery	57
Dynamic, adaptive sampling during nanopore sequencing using Bayesian experimental design	57
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	57
Efficient engineering of human and mouse primary cells using peptide-assisted genome editing	57
Multiplex de Bruijn graphs enable genome assembly from long, high-fidelity reads	56
What the COVID-19 pandemic revealed about intellectual property	56
African coronavirus surveillance network provides early warning for world	56
Democratizing knowledge representation with BioCypher	56
Efficient evolution of human antibodies from general protein language models	56
Single-cell immunology of SARS-CoV-2 infection	56
Multi-omics single-cell data integration and regulatory inference with graph-linked embedding	55
Personalized phosphoproteomics identifies functional signaling	55
Engineered live bacteria suppress Pseudomonas aeruginosa infection in mouse lung and dissolve endotracheal-tube biofilms	55
Pangenome graph construction from genome alignments with Minigraph-Cactus	55
Identifying synergistic high-order 3D chromatin conformations from genome-scale nanopore concatemer sequencing	55
A small and highly sensitive red/far-red optogenetic switch for applications in mammals	55
Robust integration of multiple single-cell RNA sequencing datasets using a single reference space	54
Estimation of tumor cell total mRNA expression in 15 cancer types predicts disease progression	54
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts	53
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	53
Inferring gene regulatory networks from single-cell multiome data using atlas-scale external data	53
Author Correction: Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning	52
Identification of mobile genetic elements with geNomad	52
Publisher Correction: Multistate and functional protein design using RoseTTAFold sequence space diffusion	52
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	52
An optimized toolkit for prime editing	52
Author Correction: Large-scale evaluation of the ability of RNA-binding proteins to activate exon inclusion	52
Soil microbiome engineering for sustainability in a changing environment	52
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	51
Publisher Correction: Drug hunters uncloak the non-coding ‘hidden’ genome	51
Publisher Correction: Light-activated genetic therapy to treat blindness enters clinic	50
Quantifying bias introduced by sample collection in relative and absolute microbiome measurements	50
An update on Cuban biotech	49
Publisher Correction: Integrative spatial analysis of cell morphologies and transcriptional states with MUSE	49
Ovarian tumor cells gain competitive advantage by actively reducing the cellular fitness of microenvironment cells	49
Author Correction: Engineered pegRNAs improve prime editing efficiency	49
A technical approach to global plant genome editing regulation	49
Author Correction: Multiscale and integrative single-cell Hi-C analysis with Higashi	49
Rapid generation of long, chemically modified pegRNAs for prime editing	48
Massively parallel phenotyping of coding variants in cancer with Perturb-seq	47
Light-activated genetic therapy to treat blindness enters clinic	47
Prediction of drug efficacy from transcriptional profiles with deep learning	47
Author Correction: An injectable bone marrow–like scaffold enhances T cell immunity after hematopoietic stem cell transplantation	47
Generative models for protein structures and sequences	47
Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap	47
Author Correction: Quantifying bias introduced by sample collection in relative and absolute microbiome measurements	47
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	47
Unidirectional single-file transport of full-length proteins through a nanopore	46
COVID-19 long haulers	46
A spatial genome aligner for resolving chromatin architectures from multiplexed DNA FISH	46