Nature Biotechnology

Article	Citations
Single-cell RNA-seq keeps cells alive	4570
Synthetic biologics provide a path to target bHLH transcription factors	2222
AI’s plastic recycling	1220
Denmark invests in mucosal vaccines	1039
Wireless microelectronic fibers to discover gut and brain pathways	968
Overcoming unpredictability in biotech patents by tailored claiming aligned with genuine enablement	785
Framework for predicting alloreactivity in hematopoietic cell transplants	732
Bite-sized solutions	539
An optimized toolkit for prime editing	537
Orchestration of secondary metabolite production in Streptomyces species	479
Author Correction: Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion	477
Human periportal liver assembloids	444
People	403
Cancer Moonshot aims at liquid biopsies	391
A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites	386
Cellular shuttles put mislocalized proteins in their right place	384
Diffusion model expands RoseTTAFold’s power	383
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope	362
Droplet-based transcriptome profiling of individual synapses	344
Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding	338
Author Correction: Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew	313
Fresh from the biotech pipeline: FDA turmoil overshadows 2025 approvals	309
Chem-map profiles drug binding to chromatin in cells	294
Engineered serum markers for non-invasive monitoring of gene expression in the brain	269
Fluorescence microscopy resolution increases to near-atomic level	261

Integrating medicine, engineering and business to educate early-stage researchers in cardiovascular device development	259
TRGT-ing the dark genome to accurately characterize tandem repeats at scale	254
Engineering innate immune cells for cancer immunotherapy	253
Biopharma dives into tumor-seeking radioactive drugs	240
Behind the graduate mental health crisis in science	238
Generative models for protein structures and sequences	238
Synthetic biology’s uncertain regulatory future in the wake of Loper Bright Enterprises v. Raimondo	237
Democratizing knowledge representation with BioCypher	234
Design of optimized epigenetic regulators for durable gene silencing with application to PCSK9 in nonhuman primates	234
Establishing a commercial solution for extremely rare genetic diseases	230
The lysosomal degraders	227
Programming biology: next-gen AI firms raise billions to design better medicines	225
Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism	224
High-efficiency transgene integration by homology-directed repair in human primary cells using DNA-PKcs inhibition	221
Programmable promoter editing for precise control of transgene expression	221
The world’s first CRISPR therapy is approved: who will receive it?	214
Automated reconstruction of whole-embryo cell lineages by learning from sparse annotations	213
Variant to function mapping at single-cell resolution through network propagation	208
Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome	202
Dual SORT LNPs for multi-organ base editing	202
Artificial intelligence-guided design of lipid nanoparticles for pulmonary gene therapy	201
Agentic AI and the rise of in silico team science in biomedical research	196
In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice	195
Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids	194
Fungal architecture is a thing, and it’s eye-catching	194
An oncolytic virus–T cell chimera for cancer immunotherapy	194
Single-cell nanobody-based profiles of multiple epigenetic modalities and chromatin velocity	192
Predicting transcriptional outcomes of novel multigene perturbations with GEARS	192
AI predicts cancer relapse from slides	191
Biotech news from around the world	191
3Q22 — more malaise in the markets	190
The need for need-finding in medical education	189
Biotech news from around the world	189
HSV-1’s contribution as a vector for gene therapy	188
People	188
Precise DNA cleavage using CRISPR-SpRYgests	183
Decoding gene regulation with CRISPR perturbations	183
Delix Therapeutics : psychedelics without the trip	178
Towards the non-invasive imaging of brain networks and functions at high resolution	176
Considerations for the future of in vitro gametogenesis in fertility care	167
Gains in early-onset dementia with progranulin open new paths for drug discovery	164
Protection of cell therapeutics from antibody-mediated killing by CD64 overexpression	163
Grand challenges in bioinformatics education and training	162
Sequence Display enables large-scale sequence–activity datasets for rapid protein evolution	160
Orna Therapeutics: circular logic	160
Computationally designed enzymes show potent catalytic activity	160
Adrestia Therapeutics — Gene networks to the rescue	156
FDA approves first MASH drug	155
Artificial miRNA slows Huntington’s	154
Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity	150

Improving metagenome binning by integrating intrinsic features and taxonomy	149
Connecting research and clinical practice: training the next generation of translational scientists	148
Live cell imaging of signaling networks using a conventional microscope	146
Mapping the patent landscape of cancer diagnostics	144
Prediction of peptide mass spectral libraries with machine learning	143
GLP-1s surprise failure in Alzheimer’s	142
Building synthetic chromosomes one yeast at a time: insights from Sc2.0	141
Next-generation psychedelics: should new agents skip the trip?	140
In vivo base editing reverses a neurodevelopmental disorder	136
Lessons from biotech’s unscientific evolution	135
Delivering 3 billion doses of Comirnaty in 2021	130
David Baltimore (1938–2025)	129
Author Correction: The cost of drug patent expiration date errors	127
Hallucinating functional protein sequences	126
A language model beats alphafold2 on orphans	126
Escaping analysis paralysis in medical training: from FRAZZLE to CALM	124
Retraction Note: Rescue of the spinal muscular atrophy phenotype in a mouse model by early postnatal delivery of SMN	122
The promises and challenges of neoantigen cancer vaccines	121
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes	121
Engineered phage with antibacterial CRISPR–Cas selectively reduce E. coli burden in mice	120
Slaughter-free meat hits the grocery shelf	119
Detection of mosaic and population-level structural variants with Sniffles2	118
A strategy to load, rethread and read protein sequences through a nanopore	118
Biotech news from around the world	117
Drug pipeline 4Q22 — sticking around	117
2022 – toughing out the trough	117
Forum: Boyden and Danzl	116
Lab-made antibody stops malaria	114
Genome-edited farm animals from haploid stem cells	113
The devolution of biosimilars regulations	112
Microbiome-friendly phages join the campaign for better antimicrobials	111
Platform solutions for commercial challenges to expanding patient access and making gene editing sustainable	111
Engineering pattern recognition receptors facilitates plant resistance breeding	110
Growth of biotech clusters over several decades through pioneering, variety and entrepreneurial science	110
Consternation follows Marks’ FDA departure	110
Editor’s pick: Red Queen Therapeutics	109
Chemigenetic kinase biosensors illuminate cell signaling networks	109
Chronic nasal congestion gets new treatment	109
Cell type inference in cell-free nucleic acid liquid biopsy	107
Quantifying endosomal escape in vivo to guide lipid nanoparticle design	107
Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments	106
The global patent landscape of optogenetics: current advances, challenges and opportunities	105
Rapid, scalable assessment of SARS-CoV-2 cellular immunity by whole-blood PCR	104
How will generative AI disrupt data science in drug discovery?	103
Improving non-viral knock-in with modified single-stranded DNAs and small molecules	103
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants	102
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors	102
RNA stability enhancers for durable base-modified mRNA therapeutics	101
Spatial host–microbiome sequencing reveals niches in the mouse gut	100
Modular cytokine receptor-targeting chimeras for targeted degradation of cell surface and extracellular proteins	100
Data sharing in the age of deep learning	99
A circularly permuted CasRx platform for efficient, site-specific RNA editing	99
Precision RNA base editing with engineered and endogenous effectors	99
Enzymatic synthesis of RNA oligonucleotides	98
Unpaired data as a first-order challenge in single-cell and spatial proteomics	98
Contamination source modeling with SCRuB improves cancer phenotype prediction from microbiome data	98
Are ecological processes that select beneficial traits in agricultural microbes nature’s intellectual property rights?	97
Epicardioid single-cell genomics uncovers principles of human epicardium biology in heart development and disease	97
An update on patent issues regarding antibody–drug conjugates	97
Large-scale discovery of chromatin dysregulation induced by oncofusions and other protein-coding variants	96
Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection	95
A fully integrated wearable ultrasound system to monitor deep tissues in moving subjects	95
2024: research in review	94
Control of mammalian gene expression by modulation of polyA signal cleavage at 5′ UTR	94
Base editors	94
Stem cells	93
An organoid model with all three pancreatic lineages resembles fetal pancreas	92
Ionis wins FDA nod for triglyceride-lowering rare disease drug	92
Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq	92
People	92
Thinking about a move from academia to industry? Look before you leap	91
Cross-species snakebite antivenom	91
Tracking gene order provides a new perspective on intraspecific evolution in microbiotas	91
Children with rare genetic diseases get CRISPR Cures center	90
Five questions with César de la Fuente	90
Author Correction: Engineering circular RNA for enhanced protein production	88
Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells	87
Gene drives tested against real-world malaria diversity	86
AI-enhanced protein design makes proteins that have never existed	86
Author Correction: Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9	86

Spotlight on cancer immunotherapies	85
Next-generation patient models for colorectal cancer research	85
Genotoxins from gut bacteria	84
Septerna: making another run on GPCRs	84
Sensitive detection of gene transfer in a microbial community	84
Generation of accurate, expandable phylogenomic trees with uDance	83
The strength and importance of government-funded patents for approved drugs	83
Discovery of drug–omics associations in type 2 diabetes with generative deep-learning models	82
Human respiratory airway progenitors derived from pluripotent cells generate alveolar epithelial cells and model pulmonary fibrosis	82
Spatial genomics of AAV vectors reveals mechanism of transcriptional crosstalk that enables targeted delivery of large genetic cargo	80
How will AI affect patent disclosures?	80
Growth-coupled microbial biosynthesis of the animal pigment xanthommatin	80
A genome-wide view of disordered proteins	80
Senolytics under scrutiny in the quest to slow aging	80
Accurate somatic small variant discovery for multiple sequencing technologies with DeepSomatic	79
Intestinal mucosal barrier repair and immune regulation with an AI-developed gut-restricted PHD inhibitor	79
Mapping medically relevant RNA isoform diversity in the aged human frontal cortex with deep long-read RNA-seq	79
Tuning plant phenotypes by precise, graded downregulation of gene expression	78
Efficient prime editing in mouse brain, liver and heart with dual AAVs	78
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase	77
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy	76
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques	76
Systema: a framework for evaluating genetic perturbation response prediction beyond systematic variation	75
The GA4GH Phenopacket schema defines a computable representation of clinical data	75
TCR cell therapies vanquish solid tumors — finally	75
High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits	75
A community effort to optimize sequence-based deep learning models of gene regulation	74
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors	74
Multifunctional microelectronic fibers enable wireless modulation of gut and brain neural circuits	74
Post-translational modifications reshape the antigenic landscape of the MHC I immunopeptidome in tumors	74
3Q23 — VCs feed ‘older children’ as IPO markets stagnate	73
People	73
2H23 biotech job picture	72
Measuring the impact of chromatin context on transcription factor binding affinities	72
Base editor restores CD3δ-dependent SCID mutation	72
Microfluidics	71
Startup grows egg proteins in potato fields	71
Microbiomics	71
Bridge RNAs direct programmable DNA rearrangements	71
Discovering the crucial function of long noncoding RNAs	71
Embryonic stem cells across bird species	70
Combining reference genomes into a pangenome graph improves accuracy and reduces bias	70
Big pharma seduced by transcription factors again. What has changed?	69
Using artificial intelligence to develop gene therapy for the lungs	69
Capturing the diversity of protein modifications on presented tumor antigens	68
Starving tumors with fat	67
Culturing microbiome therapeutics with big data	67
Gabriele et al. reply	67
Eli Lilly in $650M pact to boost muscle	67
Wearable ultrasound for continuous deep-tissue monitoring	66
AAV-delivered UGA suppressor tRNA for disease-agnostic in vivo gene therapy	65
Fresh from the biotech pipeline: FDA approvals settle in 2024, but what next?	64
Prime editing	64
A shifting market environment and strategies fueling innovation in prostate cancer treatment	64
The instant spray-on dress	64
Precise, minimally evolved adenine base editors generated through mutation reversion analysis	63
Biotech patenting 2021	63
Challenges for developing broad-based mucosal vaccines for respiratory viruses	63
Fighting fibrosis	63
Deep tissue multi-photon imaging using adaptive optics with direct focus sensing and shaping	62
Lipid nanoparticle-mediated mRNA delivery to CD34+ cells in rhesus monkeys	61
The reverse transcriptase domain of prime editors contributes to DNA repair in mammalian cells	61
Make science disruptive again	60
Resurrecting a miniature Cas9 ancestor for genome and epigenome editing	60
Generation of a live attenuated influenza A vaccine by proteolysis targeting	60
Newcomer anti-IL-33 makes strides in COPD	59
Redefining antibody patent protection using paratope mapping and CDR-scanning	58
Linking CRISPR–Cas9 double-strand break profiles to gene editing precision with BreakTag	57
Chikungunya vaccine approved	57
A germ-free humanized mouse model shows the contribution of resident microbiota to human-specific pathogen infection	57
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity	57
High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails	56
Predicting small molecule–RNA interactions without RNA tertiary structures	56
De novo detection of somatic mutations in high-throughput single-cell profiling data sets	56
A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function	56
The challenges and promise of sweat sensing	56
Uniform volumetric single-cell processing for organ-scale molecular phenotyping	55
Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases	55
Single-molecule live-cell RNA imaging with CRISPR–Csm	55
RNA interference in the era of nucleic acid therapeutics	54
Visualizing protein nanostructures in intact brain	54
Public biotech in 2021 — the numbers	54
Second-quarter biotech job picture	54
Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition	54
Biotech news from around the world	54
Forum: Locke and Sykes	53
Biotech news from around the world	53
The long game of tenure	52
A retrotransposon for site-specific gene transfer	52
Biomedical imaging	52
CRISPR comes a-knock-in to reprogram antibodies in vivo	51
Durable epigenetic silencing with a TALE-based editor	51
Fluorescence imaging of RNA and protein in deep tissue	51
Wasp gut microbes yield beer with extra tang	51
Radiopharmaceuticals	51