Preparative Biochemistry & Biotechnology

Papers
(The TQCC of Preparative Biochemistry & Biotechnology is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)
ArticleCitations
Direct and cost-effective method for histone isolation from cultured mammalian cells23
A novel strategy to construct multi-strain starter cultures: an insight to evolve from natural to directed fermentation22
Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment22
GFP fusion promotes the soluble and active expression of a pea actin isoform (PEAc1) in Escherichia coli22
Synergistic phytohormonal stimulation for enhanced carotenogenesis in Dunaliella salina20
Choice of gDNA isolation method has a significant impact on average murine Telomere Length estimates20
Purification of O-antigen for polysaccharide vaccine development against Salmonella paratyphi19
New wheat straw fermentation feed: recombinant Schizosaccharomyces pombe efficient degradation of lignocellulose and increase feed protein17
Purification and biochemical characterization of lactoperoxidase from Jersey bovine milk and kefir: inhibition and molecular modeling17
Valorization of low-grade Gracilaria verrucosa biomass via optimized acid hydrolysis: a sustainable route to high-value sugars for bioproduct development17
Production of milk-coagulating protease by fungus Pleurotus djamor through solid state fermentation using wheat bran as the low-cost substrate16
Engineered Saccharomyces cerevisiae harbors xylose isomerase and xylose transporter improves co-fermentation of xylose and glucose for ethanol production15
Biosynthesis and characterization of terpolymer P(3HB- co -3HV- co -4HB) produced by <15
UV mutagenesis for lipase overproduction from Bacillus cereus ATA179, nutritional optimization, characterization and its usability in the detergent industry15
Enzymatic modification of millet prolamin by alcalase: structural characterization and functional enhancements15
Xylanase of Bacillus sp. NIORKP76 grown under solid state fermentation: production, purification, characterization and its saccharification potential14
The effects of different extraction methods on the structure and antioxidant properties of Bletilla striata polysaccharide14
Antimicrobial peptides (AMPs) from microalgae as an alternative to conventional antibiotics in aquaculture14
Industrial-grade α-amylase production, purification, and functional characterization via co-culturing of Aspergillus niger 13
Microbial fermentation technology for degradation of saponins from peony seed meal13
Gene expression, enzyme purification and physicochemical characterization of Weissella confusa P2 glucansucrase13
Immobilization of Coprinus comatus with magnetic alginate hydrogel microsphere for improving the antioxidant activity of fermentation products12
A secretory system for extracellular production of spider neurotoxin huwentoxin-I in Escherichia coli12
Impacts of conventional and industrial wastewaters-based media on biomass production, nutrient dynamics, and fatty acid profile in a thraustochytrid culture12
Electrospun PVA based scaffolds engineered with bimetallic Ag-ZnO nanoparticles fabricated using Chromolaena odorata for wound healing applications11
Biological synthesis of Glycyrrhiza glabra -based silver nanoparticles and determination of their bioactivities11
High purity of human secreted phospholipase A2 group IIE in Pichia pastoris using basal salts medium comparison with YPD medium11
A Box-Behnken design-optimized preparative strategy for anti-hyperuricemic flavonoids from Humulus lupulus L.: from extraction to bioactivity evaluation11
Preparation of esculin acetates through transesterification reaction catalyzed by Novozyme 435 ® and their Purification followed by NMR characterization11
Enhancement in T1 lipase purification recovery using the novel construct pGEX4T1/His-T110
Optimization and purification of laccase activity from Mammaliicoccus sciuri isolated from the soils of Similipal, Odisha, India: a kinetics study of crystal violet dye 10
Optimization of chitosan-PEG-geranium oil nanoparticles and in-vitro studies on pancreatic ductal adenocarcinoma10
High-throughput clone screening and upstream process optimization for recombinant therapeutic peptide expression: biosimilar teriparatide (PTH-34): a case study10
PEGylation versus glycosylation: effect on the thermodynamics and thermostability of crisantaspase10
In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae10
Detoxification of areca nut acid hydrolysate and production of xylitol by Candida tropicalis (MTCC 6192)9
In vitro antioxidant activity evaluation of pine nut peptides ( Pinus koraiensis ) fermented by Bacillus subtilis LS-459
Response surface methodology optimization extraction of polysaccharide from Lilium brownii F.E. Brown var. viridulum 9
Enhancing methane yield and shifting microbial communities in anaerobic reactors treating lipid-rich dairy wastewater through exogenous lipase addition9
Kinetics of dye decolorization using heterogeneous catalytic system with immobilized Achromobacter xylosoxidans DDB69
Optimization of solid-state fermentation parameters and post-harvest stabilization for the mass production of Trichoderma citrinoviride EGE-K-128 micropr9
Production of reverse transcriptase from Moloney murine Leukemia virus in Escherichia coli expression system9
Optimization of medium composition of Lactobacillus plantarum Y44 using Plackett Burman and Box–Behnken designs9
Extracellular collagenase isolated from Streptomyces antibioticus UFPEDA 3421: purification and biochemical characterization9
Methane bioconversion to methanol by Methylotuvimicrobium buryatense 5GB1C: mechanistic insights using molecular simulations9
Selenium nanoparticle production using Azadirachta indica extract: biosynthesis, characterization, and cytotoxic effects in fibroblast cells9
Bidirectional fermentation of Monascus and Mulberry leaves enhances GABA and pigment contents: establishment of strategy, studies of bioactivity and mechanistic9
Studies on a new antimicrobial peptide from Vibrio proteolyticus MT1109
Insulin-transferrin-selenium promote formation of tissue-engineered vascular grafts in early stage of culture9
A systematic study on the characteristics and applications of laccases produced by fungi: insights on their potential for biotechnologies9
Selenopolysaccharides: advances in synthesis, structural characterization and biological applications9
Microencapsulation of astaxanthin-rich lipid extract from L. vannamei cephalothorax in calcium alginate matrix9
High-level production of poly-γ-glutamic acid by a newly isolated Bacillus sp. YJY-8 and potential use in increasing the production of tomato9
Aqueous two-phase extraction and characterization of thermotolerant alkaliphilic Cladophora hutchinsiae xylanase: biochemical properties and potential applications in fr8
Morphological and genomic change in lipase-producing Bacillus licheniformis NCU CS-5 induced by atmospheric room temperature plasma mutagenesis8
Biosurfactant production by Pseudomonas aeruginosa WARP_W1: kinetics and structural elucidation of glycolipids8
Extraction optimization of Phellinus baumii residue polysaccharides and investigation of their anti-aging effects via 8
Optimization, purification, and biochemical characterization of protease enzymes from Penicillium roqueforti using sunflower meal as substrate8
A green approach to natural products mediated synthesis of nanomaterial using Astragalus pennatulus , characterization, biological effect, and quantifica8
Electrochemical system for detection of fermentation-derived bioethanol via cyclic voltammetry using nickel oxide modified glassy carbon electrode8
A comprehensive study of glucose and oxygen gradients in a scaled-down model of recombinant HuGM-CSF production in thermoinduced Escherichia coli fed-batch cultures8
Peptidomic profiling of Kaempferia parviflora and Zingiber officinale and their antioxidant potentials8
Multi-functional xylanase from Aspergillus sydowii : biosynthesis of nanoconjugates, optimization by Taguchi approach and biodeinking potential8
Enhanced phytoremediation and biosorption of cationic methylene blue dye by Acidomyces acidophilus ATCC 267747
Efficient whole-cell biosynthesis of S-adenosyl-L-methionine by the engineered Escherichia coli with high ATP regenerating system7
Enhanced production of yellow fever virus through tailored culture media optimization7
Synthesis of new bio-activated carbon filled epoxy composites7
Cellulase production by Aspergillus fumigatus A4112 and the potential use of the enzyme in cooperation with surfactant to enhance floating oil recovery and methane produ7
Development of an efficient extraction and enrichment method for total flavonoids compounds from Erigeron breviscapus using ultrasound-assisted extraction and macroporous resin adsorption7
Development of a real-time PCR protocol for the detection of chicken DNA in meat products7
Measurement of yield and quality of DNA in human buffy coat is extraction method dependent7
One-pot green and sustainable process for the biotransformation of sophoricoside to genistein from Fructus Sophorae with magnetic cellulose spheres immobilized 7
Isolation, optimization, and bioactivity evaluation of Streptomyces qinglanensis VITABS23 from mangrove sediments: an in vitro and in silico study7
Multi-omics analyses reveal the regulation mechanisms of organic acid biosynthesis in newly isolated lactic acid bacteria with application in corn biomass fermentation7
Antimicrobial and antioxidant activity of green synthesized copper nanoparticles7
Optimization of lyoprotectant for recombinant human acidic fibroblast growth factor by response surface methodology7
Experimental validation off-line a nonlinear controller for removal of chromium using non-living cells of Yarrowia lipolytica7
Agro-industrial wastes revalorization as feedstock: production of lignin-modifying enzymes extracts by solid-state fermentation using white rot fungi7
Chitinase induction in Trichoderma harzianum : a solid-state fermentation approach using shrimp waste and wheat bran/commercial chitin for chitooligosaccharides synthesi6
Cost-effective, high-yield production of Pyrobaculum calidifontis DNA polymerase for PCR application6
High-productive recovery and purification of superoxide dismutase from Tetraselmis chuii using integrated ultrasonication–aqueous two-phase system6
Enhancement of bacterial cellulose production by ethanol and lactic acid by using Gluconacetobacter kombuchae6
Statistical optimization of process variables for agarase production using Microbacterium sp. SS5 strain from non-marine sources6
Preparation of magnetic polyacrylamide hydrogel with chitosan for immobilization of glutamate decarboxylase to produce γ-aminobutyric acid6
Optimization and extraction kinetic modeling of antioxidants, polyphenols, and flavonoids from Garcinia cambogia and Myristica fra6
Production of tannase from a newly isolated yeast, Geotrichum cucujoidarum using agro-residues6
Promising approaches in the extraction, characterization, and biotechnological applications of ursolic acid: a review6
Synergistic bioactivity and application of polysaccharide-based prebiotics and probiotics on animal feed, foods and medicines: a review6
Deep eutectic solvent assisted recovery of high molecular weight levan from an isolated Neobacillus pocheonensis BPSCM46
CgMRP1 improves ethanol fermentation performance of Candida glycerinogenes in undetoxified lignocellulosic hydrolysate6
Chitinase: purification and characterization from Alternaria brassicicola and its antibacterial activity6
Bioactive metabolites from edible fungi: evaluation of antioxidant potential and cytotoxicity on human cell lines6
Microbased biorefinery for gold nanoparticle production: recent advancements, applications and future aspects6
Rapid alcoholic fermentation of Candida glycerinogenes under aerobic conditions6
The scarlet metabolite: recent insights into prodigiosin biosynthesis, biological activities and potential applications – a review6
Immobilization, optimization, characterization and kinetic properties of polyphenol oxidase to multi-walled carbon nanotube6
Bio-fabrication of polymeric silver nanoparticles (Ag-NPs) using polysaccharide extracted from Ulva reticulata : structural and functional characterizati6
Characterization of a novel marine microbial uricase from Priestia flexa and evaluation of the effects of CMCS conjugation on its enzymatic properties6
Bioconversion of mustard oil cake for production of lipase, optimization and direct immobilization from solid-state fermentation extract6
Green synthesized silver nanoparticles and elicitors enhance stevioside glycoside production in the endophyte Acremonium sclerotigenum from 6
Expression of Vitreoscilla hemoglobin gene in different organisms: a new approach to increase the efficiency of various biological processes6
Optimizing the extraction of active components from Salvia miltiorrhiza by combination of machine learning models and intelligent optimization algorithms and its correla6
Biosynthesis of zinc oxide nanoparticles using Tamarix chinensis extract: evaluation of antioxidant, antimicrobial, and anti-MCF-7 cancer activities6
Enzyme inhibitory and bioactive potential of ultrasound-assisted water extract from Sideritis montana subsp. montana 6
Optimization of asymmetric bioreduction conditions of 1-(thiophen-2-yl)ethanone by Weissella cibaria N9 using a desirability function-embedded face-centered optimization5
Synergistic utilization of glucose and xylose for the myo-inositol biosynthesis in recombinant Escherichia coli BL215
Antioxidant and immunomodulatory activities of Rehmannia glutinosa extracts obtained with complex enzymes and ultrasonic wave5
The evaluation of the performance of rice husk and rice straw as potential matrix to obtain the best lipase immobilized system: creating wealth from wastes5
Antibacterial and antibiofilm activity of extracts from sponge-associated bacterial endophytes5
Harnessing algal biomass for sustainable energy: cultivation, strain improvement, and biofuel production5
A new hydrophobin candidate from Cladosporium macrocarpum with super-hydrophobic surface5
Production of Rhizopus oryzae by solid state fermentation and evaluation of its biocomposite characteristics5
Optimization of ultrasound- and enzymatic-assisted extractions of polyphenols from dried red onion peels and evaluation of their antioxidant activities5
Immobilization of Aspergillus terreus URM4658 inulinase in calcium alginate beads, evaluation of their biochemical characteristics and kinetic/thermodynamic parameters, 5
Food-grade production of recombinant human serum albumin in Kluyveromyces lactis and evaluation of its biological activity5
Evaluation of different nutrient limitation strategies for the efficient production of poly(hydroxybutyrate‐co‐hydroxyvalerate) from waste frying oil and propionic acid in high cell density fermentati5
Improving thermostability of Acinetobacter baumannii phages during cold storage through infecting naïve bacterial hosts5
Zinc oxide nanoparticle mediated modulation of antimicrobial and physico-chemical properties of essential oil containing PVA nanocomposites5
Production of a genetically encoded biosensor for evaluating enterovirus 71 3C protease inhibitors5
Exploring the antioxidant potential of fermented turmeric pulp: effect of extraction methods and microencapsulation5
Development of a novel method for recovery of intracellular biopolymer polyhydroxyalkanoate from Bacillus flexus by regulating a lambda phage lytic syste5
Enhanced production of lactosucrose using an integrated levansucrase–yeast system with an invertase-deficient Saccharomyces cerevisiae strain5
Sustainable biotransformation of acanthopanax senticosus to isofraxidin using deep eutectic solvents: decoding metabolic crosstalk through transcriptomic and metabolomic Integration5
Evaluation of the effect of different organic solvents in the efficiency of the recovery of pullulan synthesized by Aureobasidium pullulans5
Removal of orange G dye by Aspergillus niger and its effect on organic acid production5
Glassy carbon electrode modified with rice husk biochar for sodium diclofenac determination using square-wave voltammetry5
Black soybean waste as a sustainable substrate for γ-PGA production: comparative fermentation strategy and statistical process optimization5
Optimization and purification of a novel bacteriocin-like substance from Tarhana-originated Enterococcus faecium5
Enhancing the production of L-proline in recombinant Escherichia coli BL21 by metabolic engineering5
Ethanol and 2-phenylethanol production by bee-isolated Meyerozyma caribbica strains4
Ultrasonic/microwave-assisted extraction and properties of polysaccharides from Elaeagnus angustifolia4
Biopolymer curdlan production from rhizospheric bacteria Priestia aryabhattai BMS3 from Vigna radiata (mun4
Engineering Escherichia coli to metabolize sorbitol as the sole carbon source for synthesis of recombinant L-Asparaginase-II4
Isolation and characterization of heterocytes cyanobacteria and investigating their role in enhancing seed germination4
Rapid quantification of immunoglobulin G from plasma using affinity chromatography: partial validation of an efficient analytical method4
Efficient RNA extraction method for acquiring high-quality RNA from various tissues of the fiber crop abaca, Musa textilis Née4
A review on pretreatment methods for lipid extraction from microalgae biomass4
Egg shell powder: an inexpensive material for detoxification of sugarcane bagasse acid hydrolysate, cells immobilization agent and nutritional supplement for ethanol and xylitol production4
Preparation of 5-hydroxymethylfurfural from cellulose catalyzed by silicon-based composite solid acids4
Profiling functional metabolites and minerals of fortified food black gram Vigna mungo (L.) hepper4
Correction4
Immunoglobulin depletion-based affinity purification of human serum haptoglobin without mannosylated IgM co-elution4
Advancing the microalgal blue bioeconomy through technological and analytical integration4
Optimization of the extraction process of total steroids from Phillinus gilvus (Schwein.) Pat. by artificial neural network (ANN)-response surface methodology and identi4
Folic acid-conjugated biopolymeric nanoparticles for targeted delivery of ixabepilone in breast cancer4
Optimization of enzymatic production of anti-skin aging biopeptides from white sorghum [ Sorghum bicolor (L) Moench] grain4
Characterization of a novel Glutaminase-free L- asparaginase from Bifidobacterium thermophilum4
Aligned with sustainable development goals: microwave extraction of astaxanthin from wet algae and selective cytotoxic effect of the extract on lung cancer cells4
Molecular dynamics simulation and validation of spiramycin extraction using the thermosensitive polymer NPE-108/water aqueous two-phase system4
Optimizing the extraction of high-potency antioxidants from Solanum lycopersicum L. fruit: a comparative study of microwave-assisted, ultrasound-assisted4
Purification and characterization of Stenotrophomonas maltophilia chitinase with antifungal and insecticidal properties4
Role of key enzymes in the production of docosahexaenoic acid (DHA) by Thraustochytrium sp. T014
Biodegradation of polyhydroxyalkanoate film in soil: a sustainable approach for bioplastic management4
Integrated statistical and machine-learning optimization for enhanced heparosan production by Lactococcus lactis4
Oxidation and acetylation of corn cob lignin and its effects on the stabilization of Pickering emulsions4
Enhanced identification of glycoproteins and allergens in Hevea brasiliensis latex using innovative enrichment techniques4
Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus4
Preparation and optimal encapsulation of citral in proteins: effect of protein type, pH, and ratio4
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