OOIR: Observatory of International Research

Papers

(The TQCC of Protein Expression and Purification is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-11-01 to 2024-11-01.)

Article	Citations
Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry	31
Expression of SARS-CoV-2 surface glycoprotein fragment 319–640 in E. coli, and its refolding and purification	30
Linkers: A synergistic way for the synthesis of chimeric proteins	27
Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate	26
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays	24
Expression and purification of recombinant SARS-CoV-2 nucleocapsid protein in inclusion bodies and its application in serological detection	20
Stability and cytotoxicity of DPPH inhibitory peptides derived from biodegradation of chicken feather	18
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli	18
A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells	17
Medium optimization for high yield production of human serum albumin in Pichia pastoris and its efficient purification	16
Large scale production of synthetic spider silk proteins in Escherichia coli	16
Optimal secretion of thermostable Beta-glucosidase in Bacillus subtilis by signal peptide optimization	15
Purification and characterization of a cold-adapted κ-carrageenase from Pseudoalteromonas sp. ZDY3	15
Production of an invertebrate lysozyme of Scylla paramamosain in E.coli and evaluation of its antibacterial, antioxidant and anti-inflammatory effects	14
Expression and purification of S5196-272 and S6200-317 proteins from Tilapia Lake Virus (TiLV) and their potential use as vaccines	14
Reconstitution and functional characterization of SARS-CoV-2 proofreading complex	14
Soluble expression, purification, and secondary structure determination of human PDX1 transcription factor	13
Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide	13
Purification and anticancer activity of glutaminase and urease free intracellular l-asparaginase from Chaetomium sp.	12
Structural and functional characterizations and heterogenous expression of the antimicrobial peptides, Hidefensins, from black soldier fly, Hermetia illucens (L.)	12
Enzymatic properties of an efficient glucan branching enzyme and its potential application in starch modification	12
Refolded recombinant major capsid protein (MCP) from Infectious Spleen and Kidney Necrosis Virus (ISKNV) effectively stimulates serum specific antibody and immune related genes response in Nile tilapi	12
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	12
Purification and characterization of an inulinase produced by a Kluyveromyces marxianus strain isolated from blue agave bagasse	12
Improving the yield of recalcitrant Nanobodies® by simple modifications to the standard protocol	12

Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	12
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	12
Expression and purification of the antimicrobial peptide Bin1b in Escherichia coli tagged with the fusion proteins CusF3H+ and SmbP	11
Recombinant production of Trx-Ib-AMP4 and Trx-E50-52 antimicrobial peptides and antimicrobial synergistic assessment on the treatment of methicillin-resistant Staphylococcus aureus under in vitro and	11
Expression and purification of a novel single-chain diabody (scDb-hERG1/β1) from Pichia pastoris transformants	11
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	11
Characterization of a bifunctional and endolytic alginate lyase from Microbulbifer sp. ALW1 and its application in alginate oligosaccharides production from Laminaria japonica	11
In vitro preparation of uniform and nucleic acid free hepatitis B core particles through an optimized disassembly-purification-reassembly process	10
Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody	10
Improved thermostability of D-allulose 3-epimerase from Clostridium bolteae ATCC BAA-613 by proline residue substitution	10
Expression and characterization of l-arabinose isomerase from Geobacillus stearothermophilus for improved activity under acidic condition	10
Characterization of an acidic pectin methylesterase from Paenibacillus xylanexedens and its application in fruit processing	10
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	9
Characterization of a single chain variable fragment of nivolumab that targets PD-1 and blocks PD-L1 binding	9
The structure and properties of MFG-E8 and the In vitro assessment of its toxic effects on myoblast cells	9
Simultaneously targeting nitrocellulose and antibody by a dual-headed protein	9
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	9
Expression and characterization of a thermotolerant and pH-stable hyaluronate lyase from Thermasporomyces composti DSM22891	8
Effect of Clp protease from Corynebacterium glutamicum on heterologous protein expression	8
Removing light chain-missing byproducts and aggregates by Capto MMC ImpRes mixed-mode chromatography during the purification of two WuXiBody-based bispecific antibodies	8
Protein L chromatography: A useful tool for monitoring/separating homodimers during the purification of IgG-like asymmetric bispecific antibodies	8
Method for efficient soluble expression and purification of recombinant human interleukin-15	8
Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets	8
Pseudomonas aeruginosa recombinant L-asparaginase: Large scale production, purification, and cytotoxicity on THP-1, MDA-MB-231, A549, Caco2 and HCT-116 cell lines	8
Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch	8
Improvements of a high-throughput protein purification process using a calcium-dependent setup	7
Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography	7
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	7
Characterization of the Fc–III–4C-based recombinant protein expression system by using carbonic anhydrase as the model protein	7
Expression, purification and crystallization of TGW6, which limits grain weight in rice	7
Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibody	7
Expression, purification and crystallization of CLK1 kinase – A potential target for antiviral therapy	7
A generalizable protocol for expression and purification of membrane-bound bacterial phosphoglycosyl transferases in liponanoparticles	7
Improving SDS-PAGE method for monoclonal antibodies: The advantages of Tris-Acetate over Tris-Glycine SDS-PAGE system and comparison with CE-SDS method	7
Cell free protein synthesis versus yeast expression – A comparison using insulin as a model protein	7
Facile expression and purification of active human growth hormone in E. coli by a cleavable self-aggregating tag scheme	7
Biochemical characterization and detection of antitumor activity of l-asparaginase from thermophilic Geobacillus kaustophilus DSM 7263T	7
Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q	7
Optimization strategies for expression of a novel bifunctional anti-PD-L1/TGFBR2-ECD fusion protein	7
Design, expression, purification and crystallization of human 14-3-3ζ protein chimera with phosphopeptide from proapoptotic protein BAD	7
Preparation of rabbit polyclonal antibody against porcine gasdermin D protein and determination of the expression of gasdermin D in cultured cells and porcine tissues	7
Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	6
The effects of L-arginine on protein stability and DNA binding ability of SaeR, a transcription factor in Staphylococcus aureus	6
Expression and characterization of catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3	6
Characterisation and comparative analysis of hydrophobin isolated from Pleurotus floridanus (PfH)	6
Identification and characterization of a hydrophobin Vmh3 from Pleurotus ostreatus	6
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	6
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles	6
Reconstitution of the full transmembrane cadherin-catenin complex	6
Optimization of culture conditions for high-level expression of soluble and active tumor necrosis factor-α in E. coli	6

Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase	6
Heterologous expression of cobalamin dependent class-III enzymes	6
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	6
Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata	6
Heterologous expression and characterisation of a keratinase produced by Chryseobacterium carnipullorum	6
Efficient improvement of surface displayed lipase from Rhizomucor miehei in PichiaPink™ protease-deficient system	6
A simple method to purify recombinant HCV core protein expressed in Pichia pastoris for obtaining virus-like particles and producing monoclonal antibodies	6
PiP2 favors an α-helical structure of non-recombinant Hsp12 of Saccharomyces cerevisiae	6
O-acetylhomoserine sulfhydrylase from Clostridium novyi. Cloning, expression of the gene and characterization of the enzyme	6
A custom ÄKTA avant configuration enabling automated parallel protein purification over a range of process scales	6
A comparison between MBP- and NT* as N-terminal fusion partner for recombinant protein production in E. coli	6
Functional production, characterization, and immobilization of a cold-adapted cutinase from Antarctic Rhodococcus sp.	6
A thermostable glucose oxidase from Aspergillus heteromophus CBS 117.55 with broad pH stability and digestive enzyme resistance	6
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites	6
Optimization of the expression of the main protease from SARS-CoV-2	6
Development of a robust crystallization platform for immune receptor TREM2 using a crystallization chaperone strategy	5
Expression of a β-glucosidase from Trichoderma reesei in Escherichia coli using a synthetic optimized gene and stability improvements by immobilization using magnetite nano-support	5
Effect of cell-permeable grouper Manganese Superoxide Dismutase on environmental stress in fish	5
Comparison of three palm tree peroxidases expressed by Escherichia coli: Uniqueness of African oil palm peroxidase	5
Viral removal by column chromatography in downstream processing of monoclonal antibodies	5
MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs	5
Removing a light-chain missing byproduct by MMC ImpRes mixed-mode chromatography under weak partitioning mode in purifying a WuXiBody-based bispecific antibody	5
Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system	5
High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system	5
Removing a difficult-to-separate byproduct by Capto L affinity chromatography during the purification of a WuXiBody-based bispecific antibody	5
Expression and purification of a recombinant ELRL-MAP30 with dual-targeting anti-tumor bioactivity	5
Production of active recombinant human aldehyde oxidase (AOX) in the baculovirus expression vector system (BEVS) and deployment in a pre-clinical fraction-of-control AOX compound exposure assay	5
Recombinant expression of the precursor of rat lung surfactant protein B in Escherichia coli and its antibacterial mechanism	5
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation	5
Biochemical characterization of LysVpKK5 endolysin from a marine vibriophage	5
Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation	5
An efficient system to generate truncated human angiotensin converting enzyme 2 (hACE2) capable of binding RBD and spike protein of SARS-CoV2	5
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	5
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	5
Biochemical characterization and redesign of the coenzyme specificity of a novel monofunctional NAD+-dependent homoserine dehydrogenase from the human pathogen Neisseria gonorrhoeae	5
Optimizing purification of the peripheral membrane protein FAM92A1 fused to a modified spidroin tag	5
Expression in Escherichia coli, purification and kinetic characterization of LAPLm, a Leishmania major M17-aminopeptidase	5
The choice of chromatographic resin for the purification of recombinant lysostaphin affects its activity	5
Enhancing the secretion of a feruloyl esterase in Bacillus subtilis by signal peptide screening and rational design	5
Bioconversion of recombinantly produced precursor peptide pqqA into pyrroloquinoline quinone (PQQ) using a cell-free in vitro system	5
cDNA cloning, prokaryotic expression, and functional analysis of squalene synthase (SQS) in Camellia vietnamensis Huang	5
Expression of rice β-exoglucanase II (OsExoII) in Escherichia coli, purification, and characterization	5
Metalation of a rice type 1 metallothionein isoform (OsMTI-1b)	5
Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2	5
Co-expression of recombinant human collagen α1(III) chain with viral prolyl 4-hydroxylase in Pichia pastoris GS115	5
Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli	5
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	5
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	5
Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact	5
In vitro translation of virally-encoded replication polyproteins to recapitulate polyprotein maturation processes	5
Optimization of the heterologous expression and purification of Plasmodium falciparum generative cell specific 1 in Escherichia coli	4
Adapting recombinant bacterial alkaline phosphatase for nucleotide exchange of small GTPases	4
Co-expression of self-cleaved multiple proteins derived from Porcine Reproductive and Respiratory Syndrome Virus by bi-cistronic and tri-cistronic DNA vaccines	4
How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger	4
One-step metal affinity purification of recombinant hFGF19 without using tags	4
Cell-free protein synthesis of CD1E and B2M protein and in vitro interaction	4
A comparative study and evaluation of anti-EGFR nanobodies expressed in Pichia pastoris and Escherichia coli as antitumor moieties	4
Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles	4
Generation of a soluble and stable apoptin-EGF fusion protein, a targeted viral protein applicable for tumor therapy	4
Identification of chicken-derived scFv against N-glycolylneuraminic acid retrieved from an immune library by phage display	4
Recombinant expression and characterization of Oryctolagus cuniculus chymosin in Komagataella phaffii (Pichia pastoris)	4
Enhanced expression and purification of nucleotide-specific ribonucleases MC1 and Cusativin	4
Expression, purification and characterization of the IcmG and IcmK proteins of the type IVB secretion system from Coxiella burnetii	4
Capture and purification of an untagged nanobody by mixed weak cation chromatography and cation exchange chromatography	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4
High-level extracellular production and immobilisation of methyl parathion hydrolase from Plesiomonas sp. M6 expressed in Pichia pastoris	4
Rational design of a Nivolumab-based ANTI-PD-1 single chain variable fragment that blocks the interaction between PD-1 expressed on T-CELLS and PD-L1 ON CHO cells	4
Expression of HCV genotype-4 core antigen in prokaryotic E. coli system for diagnosis of HCV infection in Egypt	4
Prediction of improved therapeutics for fabry disease patients generated by mutagenesis of the α-galactosidase A active site, dimer interface, and glycosylation region	4
Light-induced expression of a novel marine laccase in Escherichia coli from Marinomonas profundimaris and its application in synthetic dye decolorization	4
Production of a human mitochondrial ABC transporter in E. coli	4
Large scale purification and characterization of A21 deamidated variant-most prominent post translational modification (PTM) for insulins which is also widely observed in insulins pharmaceutical manuf	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Antibody aggregation can lead to reduced Protein A binding capacity and low step yield	4
Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2	4
Production of functional recombinant prolyl hydroxylase-2 enzyme in insect cells for small molecule inhibitor screening studies	4
Effects of removing a highly conserved disulfide bond in ubiquitin-associated domain of human HOIP on biochemical characteristics	4
A scalable and reproducible manufacturing process for Phlebotomus papatasi salivary protein PpSP15, a vaccine candidate for leishmaniasis	4
Two-step purification of tag-free norovirus-like particles from silkworm larvae (Bombyx mori)	4