Protein Expression and Purification

Article	Citations
Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli	31
Optimization of the expression of the main protease from SARS-CoV-2	31
Protein inclusion into ice can dissociate subunits	27
α-Conotoxin recombinant protein ImI-AFP3 efficiently inhibits the growth and migration of lung cancer cells	26
Cloning and expression of Neurospora crassa cellobiohydrolase II in Pichia pastoris	24
Plant cellulose synthase membrane protein isolation directly from Pichia pastoris protoplasts, liposome reconstitution, and its enzymatic characterization	20
Expression of blue pigment synthetase a from Streptomyces lavenduale reveals insights on the effects of refactoring biosynthetic megasynthases for heterologous expression in Escherichia coli.	18
Process development and characterization of recombinant nucleocapsid protein for its application on COVID-19 diagnosis	17
Editorial Board	16
A procedure for producing an anti-AXL nanobody in E. coli	16
Purification, crystallization and preliminary crystallographic analysis of Chlamydophila pneumoniae AP endonuclease IV	15
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	15
Genetically encoded site-specific 19F unnatural amino acid incorporation in V. natriegens for in-cell NMR analysis	15
Purification and characterization of tomato arginine decarboxylase and its inhibition by the bacterial small molecule phevamine A	15
Identification and characterization of a novel sativene synthase from Fischerella thermalis	14
Cytoplasmic production of Fabs in chemically defined media in fed-batch fermentation	14
Editorial Board	13
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	13
Editorial Board	12
Chemometric modeling of the electrochemical data to investigate proline cis/trans isomeration effect on aggregation of Tau protein	12
Expression in Escherichia coli, purification and kinetic characterization of LAPLm, a Leishmania major M17-aminopeptidase	12
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	12
Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch	12
Generation and characterization of a humanized anti-IL-17A rabbit monoclonal antibody	11
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	11

Evaluating a new mixed-mode resin Diamond MMC Mustang using Capto MMC ImpRes as a benchmark	11
Expression of HCV genotype-4 core antigen in prokaryotic E. coli system for diagnosis of HCV infection in Egypt	11
Molecular cloning and biochemical characterization of a trehalose synthase from Myxococcus sp. strain V11	11
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	10
Expression, purification, and study on the efficiency of a new potent recombinant scFv antibody against the SARS-CoV-2 spike RBD in E. coli BL21	10
Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies	10
Recombinant expression and molecular characterization of buffalo sperm lysozyme-like protein 1	10
Preparation and characterization of a high-affinity monoclonal antibody against nerve growth factor	10
Effects of removing a highly conserved disulfide bond in ubiquitin-associated domain of human HOIP on biochemical characteristics	10
Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles	9
One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system	9
Purification, crystallization, and X-ray diffraction analysis of myocyte enhancer factor 2D and DNA complex	8
Design and construction of a phage-displayed Camelid nanobody library using a simple bioinformatics method	8
Recombinant expression, purification and characterization of an active bacterial feruloyl-CoA synthase with potential for application in vanillin production	8
NADP+-dependent l-arginine dehydrogenase from Pseudomonas velonii: Purification, characterization and application to an l-arginine assay	8
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	7
Enhanced expression and solubility of main protease (Mpro) of SARS-CoV-2 from E. coli	7
Biochemical characterization and redesign of the coenzyme specificity of a novel monofunctional NAD+-dependent homoserine dehydrogenase from the human pathogen Neisseria gonorrhoeae	7
Isolation of recombinant apolipoprotein E4 N-terminal domain by foam fractionation	7
Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets	7
Heterologous expression of a novel galactose-1-phosphate uridylyltransferase from Thermodesulfatator indicus and its application for bioproduction of Gal-β-1,4-GlcNAc-X	7
Purification and HDL-like particle formation of apolipoprotein A-I after co-expression with the EDDIE mutant of Npro autoprotease	7
The calcium-binding photoprotein clytin II: Expression of the preferred human codon-optimized clytin II gene in Chinese hamster ovary-K1 cells and its use in the G-protein-coupled receptor assays	7
Optimization strategies for expression of a novel bifunctional anti-PD-L1/TGFBR2-ECD fusion protein	7
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	7
CHO cathepsin B identified as the protease responsible for a target bispecific antibody fragmentation	7
Characterization of a highly thermostable recombinant xylanase from Anoxybacillus ayderensis	7
Large scale production of synthetic spider silk proteins in Escherichia coli	6
Optimal secretion of thermostable Beta-glucosidase in Bacillus subtilis by signal peptide optimization	6
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	6
Reconstitution of the full transmembrane cadherin-catenin complex	6
Rational design of a Nivolumab-based ANTI-PD-1 single chain variable fragment that blocks the interaction between PD-1 expressed on T-CELLS and PD-L1 ON CHO cells	6
Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein	6
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	6
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli	6
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	6
Sampling the cultivation parameter space for the bacterial production of TLR1 intracellular domain reveals the multiple optima	6
Enzymatic characterization and thermostability improvement of an acidophilic endoxylanase PphXyn11 from Paenibacillus physcomitrellae XB	6
Screening of six cation exchange resins for high binding capacity, monomer purity and step yield: A case study	6
Expression and purification of an NP-hoc fusion protein: Utilizing influenza a nucleoprotein and phage T4 hoc protein	6
Tick-borne encephalitis nonstructural protein NS1 expressed in E. coli retains immunological properties of the native protein	6
A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells	6
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles	6
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica	6
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	6
CUREs for high-level Galectin-3 expression	6
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	6
Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact	6
A generalizable protocol for expression and purification of membrane-bound bacterial phosphoglycosyl transferases in liponanoparticles	5
Characterization of an acidic pectin methylesterase from Paenibacillus xylanexedens and its application in fruit processing	5

Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2	5
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites	5
Efficient production of recombinant human FVII in CHO cells using the piggyBac transposon system	5
Enzymatic and antibacterial activity of the recombinant endolysin PVP-SE1gp146 expressed in Hansenula polymorpha	5
Editorial Board	5
A simple method to purify recombinant HCV core protein expressed in Pichia pastoris for obtaining virus-like particles and producing monoclonal antibodies	5
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
Use of waste frying oil and coconut pulp for the production, isolation, and characterization of a new lipase from Moesziomyces aphidis	5
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays	5
Editorial Board	5
Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q	5
Linkers: A synergistic way for the synthesis of chimeric proteins	5
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	5
Removal and monitoring of residual nucleic acids from core streptavidin inclusion bodies for increased refolding yield	5
Biochemical and functional characterization of the N-terminal ubiquitin-like domain of human SHARPIN	5
Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33	5
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	5
Improved thermostability of D-allulose 3-epimerase from Clostridium bolteae ATCC BAA-613 by proline residue substitution	5
Efficient periplasmic expression of active lysyl endopeptidase and optimizing the purification methods	5
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	5
Tandem fusion of albumin-binding domains promoted soluble expression and stability of recombinant trichosanthin in vitro and in vivo	5
Enhancing the thermostability of transglutaminase from Streptomyces mobaraensis based on the rational design of a disulfide bond	5
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	5
Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry	5
Expression, purification, and refolding of the recombinant extracellular domain β1-subunit of the dog Na+/K+-ATPase of the epithelial cells	5
Biochemical and structural characterization of a novel L-isoleucine-4-dioxygenase (RaIDO) from Rahnella aquatilis	5
Expression and purification of S5196-272 and S6200-317 proteins from Tilapia Lake Virus (TiLV) and their potential use as vaccines	5
Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system	5
Enhancement of recombinant human interleukin-22 production by fusing with human serum albumin and supplementing N-acetylcysteine in Pichia Pastoris	4
Chagasin from Trypanosoma cruzi as a molecular scaffold to express epitopes of TSA-1 as soluble recombinant chimeras	4
Enzymatic properties of an efficient glucan branching enzyme and its potential application in starch modification	4
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	4
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	4
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)	4
Identification and characterization of Poly(ADP-ribose) polymerase-1 interacting proteins during development of Dictyostelium discoideum	4
Editorial Board	4
Robust production of active Ulp1 (SUMO protease) from inclusion bodies	4
Overexpression and truncation of a novel cold-adapted lipase with improved enzymatic characteristics	4
Cloning and characterization of a novel DNase gene from Trichogramma pretiosum	4
Discovery and characterization of hydroxylysine O-glycosylation in an engineered IL-2 fusion protein	4
Editorial Board	4
Effect of Clp protease from Corynebacterium glutamicum on heterologous protein expression	4
Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	4
Editorial Board	4
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	4
Expression and purification of human interferon alpha 2a (IFNα2a) in the methylotrophic yeast Pichia pastoris	4
Effect of tandem repeats of antimicrobial peptide CC34 on production of target proteins and activity of Pichia pastoris	4
Complementary methods for monitoring hole-hole homodimer associated with a WuXiBody-based asymmetric bispecific antibody	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	4
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	4
Removing a light-chain missing byproduct by MMC ImpRes mixed-mode chromatography under weak partitioning mode in purifying a WuXiBody-based bispecific antibody	4
An improved production and purification protocol for recombinant soluble human fibroblast activation protein alpha	4
Developing a secretory AcGFP1-based IRES expression system for efficient production of mammalian recombinant proteins	4
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	3
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	3
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	3
Synergetic interaction of capsid proteins for virus-like particles assembly of foot-and-mouth disease virus (serotype O) from the inclusion bodies	3
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	3
Expression, purification and characterization of TMCO1 for structural studies	3
Recombinant highly antigenic truncated fusion-based protein as a diagnostic antigen for anti-SARS-CoV-2 nucleocapsid antibody ELISA	3
Editorial Board	3
Editorial Board	3
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	3
Alternative strategies for the recombinant synthesis, DOPA modification and analysis of mussel foot proteins – A case study for Mefp-3 from Mytilus edulis	3
A comparative study and evaluation of anti-EGFR nanobodies expressed in Pichia pastoris and Escherichia coli as antitumor moieties	3
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	3
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	3
Editorial Board	3
Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy	3
Co-expression of recombinant human collagen α1(III) chain with viral prolyl 4-hydroxylase in Pichia pastoris GS115	3
Expression, purification, characterization, and patient IgE reactivity of new macadamia nut iso-allergen	3
Recombinant expression of Yersinia ruckeri outer membrane proteins in Escherichia coli extracellular vesicles	3
Efficient improvement of surface displayed lipase from Rhizomucor miehei in PichiaPink™ protease-deficient system	3
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	3
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	3
Purification and characterization of an FeII- and α-ketoglutarate-dependent xanthine hydroxylase from Aspergillus oryzae	3
Expression of soluble recombinant human matrix metalloproteinase 9 and generation of its monoclonal antibody	3
Expression, purification and refolding of pro-MMP-2 from inclusion bodies of E. coli	3

High performance production process development and scale-up of an anti-TSLP nanobody	3
Time- and cost-efficient bacterial expression and purification of potato apyrase	3
Glutathione synthetase from Hydra vulgaris: Molecular cloning, overexpression, purification and partial characterization	3
Recombinant production of antibody antigen-binding fragments with an N-terminal human growth hormone tag in mammalian cells	3
High yield expression in Pichia pastoris of human neutrophil elastase fused to cytochrome B5	3
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	3
Expression, purification, and characterization of pneumococcal PsaA-PspA fusion protein	3
Editorial Board	3
Rapid purification of rabbit immunoglobulins using a single-step, negative-selection chromatography	3
Production of functional recombinant prolyl hydroxylase-2 enzyme in insect cells for small molecule inhibitor screening studies	3