OOIR: Observatory of International Research

Papers

(The TQCC of Protein Expression and Purification is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)

Article	Citations
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	38
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	37
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	32
Generation and characterization of a humanized anti-IL-17A rabbit monoclonal antibody	26
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	23
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	21
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	21
Protein inclusion into ice can dissociate subunits	20
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	20
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	18
Optimization of the expression of the main protease from SARS-CoV-2	18
High performance production process development and scale-up of an anti-TSLP nanobody	16
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	16
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	16
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	16
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	16
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	15
Editorial Board	15
Editorial Board	15
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	14
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	14
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	13
Preparation and characterization of LGR5 LOOP region-specific nanobodies	13
Purification and characterization of the intrinsically disordered Arabidopsis thaliana protein SOG1	13
Simplified purification process of AsnB3 deamidated insulin variant and its comparison with human insulin	12

Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	11
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	11
Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2	10
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	10
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	10
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	10
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	10
Purification and characterization of an FeII- and α-ketoglutarate-dependent xanthine hydroxylase from Aspergillus oryzae	10
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	10
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	10
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	9
Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	9
One-step metal affinity purification of recombinant hFGF19 without using tags	8
Production of recombinant human ameloblastin by a fully native purification pathway	8
Strategies for rapid production of crystallization quality coatomer WD40 domains	8
Development of an effective method for purifying trypsin using a recombinant inhibitor	8
Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata	8
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose	8
High-level extracellular production and immobilisation of methyl parathion hydrolase from Plesiomonas sp. M6 expressed in Pichia pastoris	8
Editorial Board	8
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	8
Molecular chaperone GroEL-GroES enhances the soluble expression of biologically active ovine growth hormone in the prokaryotic system	8
Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli	8
Biochemical characterization of a novel bifunctional alginate lyase from Microbulbifer arenaceous	7
Bacterial expression, purification and characterization of the human Dectin-1 lectin domain for structural and functional studies	7
Synthesis of BLF1-containing trimethyl chitosan nanoparticles and evaluation of its immunogenicity and protection in syrian mice by oral and subcutaneous injections	7
Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli	7
Overproduction, purification, and transcriptional activity of recombinant Acinetobacter baylyi ADP1 RNA polymerase holoenzyme	7
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum	7
Purification and characterization of recombinant human superoxide dismutase integrated with resilin-like polypeptide	7
Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR	7
Editorial Board	7
Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations	7
A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing	7
Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system	7
Expression, characterization and anti-colon cancer activity of recombinant ginseng peptides with amino acid tandem repeats	7
Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant	7
Advantage of leaky expression, acid solubilization and CHAPS in the production of cost-effective bone morphogenetic Protein-2	7
Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli	7
An important consideration when expressing mAbs in Escherichia coli	7
High yield expression and purification of full-length Neurotensin with pyroglutamate modification	6
Expression and purification of a novel single-chain diabody (scDb-hERG1/β1) from Pichia pastoris transformants	6
Development of a bispecific Nanobody anti-F17 fimbria as a potential therapeutic tool	6
A multidomain PARP14 construct suitable for bacterial expression	6
Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions	6
Editorial Board	6
Deepening in the understanding of the role of collagen subunits on the differential molecular arrangement of P. glauca and M. merluccius marine collagens	6
A protocol for production of perdeuterated OmpF porin for neutron crystallography	6
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli	6
Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	6

The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice	6
Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds	6
Unlocking the potential of Extensin Signal peptide and Elastin-like polypeptide tag fused to Shigella dysenteriae's IpaDSTxB to improve protein expression and purification in Nicotiana tabacum and Med	6
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm	6
Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells	6
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	5
Editorial Board	5
Differentially labeled flaviviral protease-cofactor complex for NMR spectroscopic applications	5
Expression, purification, characterization and crystallization of Panax quinquefolius ginsenoside glycosyltransferase Pq3-O-UGT2	5
Preparation and characterization of a novel humanized collagen III with repeated fragments of Gly300-Asp329	5
Affinity gel synthesis from the p-aminobenzoic acid derivative 4-amino-2-methylbenzoic acid and purification of polyphenol oxidase from various plant sources	5
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
CUREs for high-level Galectin-3 expression	5
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	5
Uncovering the role of leucine 59 in Renilla luciferase stability and activity with error-prone PCR: Implications for protein engineering	5
Construction of a novel TROP2/CD3 bispecific antibody with potent antitumor activity and reduced induction of Th1 cytokines	5
Soluble expression and purification of recombinant bovine ferritin H-chain	5
Purification and characterization of an asialofetuin specific lectin from the rhizome of Xanthosoma violaceum Schott	5
Corrigendum to “High-affinity nanobodies targeting IL-12B for the detection of fluorescence resonance energy transfer” [Protein Express. Purificat. 229 (2025) 106681]	5
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	5
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	5
Editorial Board	5
Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein	5
High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system	5
Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids	5
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation	5
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	5
Preparation of recombinant neuritin protein	5
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	5
Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens	4
Soluble expression of recombinant human interleukin-2 in Escherichia coli and its facile production	4
A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins	4
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)	4
Time- and cost-efficient bacterial expression and purification of potato apyrase	4
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	4
A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro	4
Unravelling the reason for different binding behaviors exhibited by antibody aggregates towards preparative and analytical Protein A columns	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation	4
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	4
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease	4
Identification of host cell proteins that interact with the M protein of Pigeon paramyxovirus type 1	4
A novel method for synthesizing authentic SARS-CoV-2 main protease	4
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	4
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	4
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense	4
Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli	4
Fusion with CTP increases the stability of recombinant neuritin	4
High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP	4
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination	4
Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2	4
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	4
A procedure for producing an anti-AXL nanobody in E. coli	4
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	4
Functional expression of the chimera proteins of Nav1.7 and NavAb in Escherichia coli	4
Optimization strategies for expression and purification of soluble N-terminal domain of human centriolar protein SAS-6 in Escherichia coli	4