Protein Expression and Purification

Article	Citations
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays	78
Recombinant expression of nanobodies and nanobody-derived immunoreagents	66
Cloning, expression and characterization of a thermo-alkali-stable xylanase from Aspergillus oryzae LC1 in Escherichia coli BL21(DE3)	27
A new insight into purification of polyphenol oxidase and inhibition effect of curcumin and quercetin on potato polyphenol oxidase	27
Expression of SARS-CoV-2 surface glycoprotein fragment 319–640 in E. coli, and its refolding and purification	24
Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry	24
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays	22
Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate	20
Refolding, characterization, and dye decolorization ability of a highly thermostable laccase from Geobacillus sp. JS12	20
Expression, purification and characterization of a cold-adapted dextranase from marine bacteria and its ability to remove dental plaque	20
Production of membrane proteins in industry: The example of GPCRs	18
Linkers: A synergistic way for the synthesis of chimeric proteins	18
Enhancing soluble expression of sucrose phosphorylase in Escherichia coli by molecular chaperones	18
Stability and cytotoxicity of DPPH inhibitory peptides derived from biodegradation of chicken feather	17
Purification, biochemical characterization and DNA protection against oxidative damage of a novel recombinant superoxide dismutase from psychrophilic bacterium Halomonas sp. ANT108	16
Expression and purification of recombinant SARS-CoV-2 nucleocapsid protein in inclusion bodies and its application in serological detection	16
A potential downstream platform approach for WuXiBody-based IgG-like bispecific antibodies	15
Skimmed milk as an alternative for IPTG in induction of recombinant protein expression	15
Purification and characterization of a cold-adapted κ-carrageenase from Pseudoalteromonas sp. ZDY3	14
Medium optimization for high yield production of human serum albumin in Pichia pastoris and its efficient purification	14
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli	13
Heterologous production of porcine derived antimicrobial peptide PR-39 in Escherichia coli using SUMO and intein fusion systems	13
Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide	13
Optimal secretion of thermostable Beta-glucosidase in Bacillus subtilis by signal peptide optimization	13
Soluble expression, purification, and secondary structure determination of human PDX1 transcription factor	13

Affimers as an alternative to antibodies for protein biomarker enrichment	13
Enhancing the yield of human erythropoietin in Aspergillus niger by introns and CRISPR-Cas9	13
Reconstitution and functional characterization of SARS-CoV-2 proofreading complex	13
Production of an invertebrate lysozyme of Scylla paramamosain in E.coli and evaluation of its antibacterial, antioxidant and anti-inflammatory effects	13
A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells	13
Expression and purification of the antimicrobial peptide Bin1b in Escherichia coli tagged with the fusion proteins CusF3H+ and SmbP	12
Purification and characterization of an inulinase produced by a Kluyveromyces marxianus strain isolated from blue agave bagasse	12
Non-chromatographic purification of thermostable endoglucanase from Thermotoga maritima by fusion with a hydrophobic elastin-like polypeptide	11
Expression and purification of S5196-272 and S6200-317 proteins from Tilapia Lake Virus (TiLV) and their potential use as vaccines	11
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	11
Expression and purification of a novel single-chain diabody (scDb-hERG1/β1) from Pichia pastoris transformants	10
A lectin with anti-microbial and anti proliferative activities from Lantana camara, a medicinal plant	10
Expression and characterization of a chitinase from Serratia marcescens	10
Cloning, expression and purification of the low-complexity region of RanBP9 protein	10
Removing half antibody byproduct by Protein A chromatography during the purification of a bispecific antibody	10
Enzymatic properties of an efficient glucan branching enzyme and its potential application in starch modification	10
Purification and anticancer activity of glutaminase and urease free intracellular l-asparaginase from Chaetomium sp.	10
Expression and characterization of l-arabinose isomerase from Geobacillus stearothermophilus for improved activity under acidic condition	10
In vitro preparation of uniform and nucleic acid free hepatitis B core particles through an optimized disassembly-purification-reassembly process	10
Large scale production of synthetic spider silk proteins in Escherichia coli	10
Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody	9
Characterization of a bifunctional and endolytic alginate lyase from Microbulbifer sp. ALW1 and its application in alginate oligosaccharides production from Laminaria japonica	9
Structural and functional characterizations and heterogenous expression of the antimicrobial peptides, Hidefensins, from black soldier fly, Hermetia illucens (L.)	9
Development of a monoclonal antibody against canine parvovirus NS1 protein and investigation of NS1 dynamics and localization in CPV-infected cells	9
Starch-degrading enzymes from the brown-rot fungus Fomitopsis palustris	9
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	9
Refolded recombinant major capsid protein (MCP) from Infectious Spleen and Kidney Necrosis Virus (ISKNV) effectively stimulates serum specific antibody and immune related genes response in Nile tilapi	9
Cloning and characterization of the Bambusa oldhamii BoMDH-encoded malate dehydrogenase	9
Molecular cloning, characterization, and heterologous expression of an acetyl-CoA acetyl transferase gene from Sanghuangporus baumii	8
Nanobody production can be simplified by direct secretion from Escherichia coli	8
Simultaneously targeting nitrocellulose and antibody by a dual-headed protein	8
Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets	8
Modified chemical method for efficient transformation and diagnosis in Pichia pastoris	8
An improved method for the expression and purification of porcine dihydropyrimidine dehydrogenase	8
Improving the yield of recalcitrant Nanobodies® by simple modifications to the standard protocol	8
Expression, purification, and characterization of recombinant apoPholasin	8
Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch	8
Separating antibody species containing one and two kappa light chain constant region by KappaSelect affinity chromatography	8
Protein L chromatography: A useful tool for monitoring/separating homodimers during the purification of IgG-like asymmetric bispecific antibodies	8
A novel recombinant expression and purification approach for the full-length anti-apoptotic membrane protein Bcl-2	8
The structure and properties of MFG-E8 and the In vitro assessment of its toxic effects on myoblast cells	8
Characterization of an acidic pectin methylesterase from Paenibacillus xylanexedens and its application in fruit processing	8
Removing light chain-missing byproducts and aggregates by Capto MMC ImpRes mixed-mode chromatography during the purification of two WuXiBody-based bispecific antibodies	8
Recombinant production of Trx-Ib-AMP4 and Trx-E50-52 antimicrobial peptides and antimicrobial synergistic assessment on the treatment of methicillin-resistant Staphylococcus aureus under in vitro and	7
Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	7
Characterization of a single chain variable fragment of nivolumab that targets PD-1 and blocks PD-L1 binding	7
Biochemical characterization and detection of antitumor activity of l-asparaginase from thermophilic Geobacillus kaustophilus DSM 7263T	7
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	7
Optimization strategies for expression of a novel bifunctional anti-PD-L1/TGFBR2-ECD fusion protein	7
Preparation of rabbit polyclonal antibody against porcine gasdermin D protein and determination of the expression of gasdermin D in cultured cells and porcine tissues	7

Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography	7
Novel strategy for expression and characterization of rabies virus glycoprotein	7
Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates	6
Expression, purification and crystallization of CLK1 kinase – A potential target for antiviral therapy	6
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	6
Design, expression, purification and crystallization of human 14-3-3ζ protein chimera with phosphopeptide from proapoptotic protein BAD	6
Characterization of the Fc–III–4C-based recombinant protein expression system by using carbonic anhydrase as the model protein	6
Purification of native CCL7 and its functional interaction with selected chemokine receptors	6
Improvements of a high-throughput protein purification process using a calcium-dependent setup	6
Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata	6
Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q	6
A thermostable glucose oxidase from Aspergillus heteromophus CBS 117.55 with broad pH stability and digestive enzyme resistance	6
Highly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro encapsulation of ruthenium nanoparticles for catalysis	6
PiP2 favors an α-helical structure of non-recombinant Hsp12 of Saccharomyces cerevisiae	6
The effects of L-arginine on protein stability and DNA binding ability of SaeR, a transcription factor in Staphylococcus aureus	6
Expression, purification and crystallization of TGW6, which limits grain weight in rice	6
Cell-free expression of natively folded hydrophobins	6
Effect of Clp protease from Corynebacterium glutamicum on heterologous protein expression	6
Method for efficient soluble expression and purification of recombinant human interleukin-15	6
Production of bioactive human IFN-γ protein by agroinfiltration in tobacco	6
Heterologous expression of cobalamin dependent class-III enzymes	6
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	6
Recombinant protein production and purification of SiiD, SiiE and SiiF - Components of the SPI4-encoded type I secretion system from Salmonella Typhimurium	6
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	5
Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact	5
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	5
Removing a difficult-to-separate byproduct by Capto L affinity chromatography during the purification of a WuXiBody-based bispecific antibody	5
Expression, purification, and characterization of human mannose-6-phosphate receptor – Extra cellular domain from a stable cell line utilizing a small molecule biomimetic of the mannose-6-phosphate mo	5
cDNA cloning, prokaryotic expression, and functional analysis of squalene synthase (SQS) in Camellia vietnamensis Huang	5
Bioconversion of recombinantly produced precursor peptide pqqA into pyrroloquinoline quinone (PQQ) using a cell-free in vitro system	5
Comparison of three palm tree peroxidases expressed by Escherichia coli: Uniqueness of African oil palm peroxidase	5
Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation	5
Viral removal by column chromatography in downstream processing of monoclonal antibodies	5
Identification and characterization of a hydrophobin Vmh3 from Pleurotus ostreatus	5
Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibody	5
Efficient improvement of surface displayed lipase from Rhizomucor miehei in PichiaPink™ protease-deficient system	5
Enhancing the thermostability of transglutaminase from Streptomyces mobaraensis based on the rational design of a disulfide bond	5
Expression and purification of a recombinant ELRL-MAP30 with dual-targeting anti-tumor bioactivity	5
Peptidyl-prolyl isomerase and the biological activities of recombinant protein cyclophilin from Pyropia yezoensis (PyCyp)	5
A custom ÄKTA avant configuration enabling automated parallel protein purification over a range of process scales	5
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation	5
Recombinant expression of the precursor of rat lung surfactant protein B in Escherichia coli and its antibacterial mechanism	5
Facile expression and purification of active human growth hormone in E. coli by a cleavable self-aggregating tag scheme	5
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	5
Functional production, characterization, and immobilization of a cold-adapted cutinase from Antarctic Rhodococcus sp.	5
Characterization of a hyperphosphorylated variant of G protein-coupled receptor kinase 5 expressed in E. coli	5
Removing a light-chain missing byproduct by MMC ImpRes mixed-mode chromatography under weak partitioning mode in purifying a WuXiBody-based bispecific antibody	5
A generalizable protocol for expression and purification of membrane-bound bacterial phosphoglycosyl transferases in liponanoparticles	5
Expression and characterization of a thermotolerant and pH-stable hyaluronate lyase from Thermasporomyces composti DSM22891	5
Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli	5
Optimizing purification of the peripheral membrane protein FAM92A1 fused to a modified spidroin tag	5
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles	5
Cell free protein synthesis versus yeast expression – A comparison using insulin as a model protein	5
Pseudomonas aeruginosa recombinant L-asparaginase: Large scale production, purification, and cytotoxicity on THP-1, MDA-MB-231, A549, Caco2 and HCT-116 cell lines	5
Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase	5
Enhanced expression and purification of nucleotide-specific ribonucleases MC1 and Cusativin	5
A comparison between MBP- and NT* as N-terminal fusion partner for recombinant protein production in E. coli	5
Affinity tag effect on the salt rejection potential of Halomonas elongata aquaporin incorporated in thin film nanocomposite membrane	5
An efficient system to generate truncated human angiotensin converting enzyme 2 (hACE2) capable of binding RBD and spike protein of SARS-CoV2	5
Reconstitution of the full transmembrane cadherin-catenin complex	4
Biochemical characterization and redesign of the coenzyme specificity of a novel monofunctional NAD+-dependent homoserine dehydrogenase from the human pathogen Neisseria gonorrhoeae	4
Two-step purification of tag-free norovirus-like particles from silkworm larvae (Bombyx mori)	4
Improving SDS-PAGE method for monoclonal antibodies: The advantages of Tris-Acetate over Tris-Glycine SDS-PAGE system and comparison with CE-SDS method	4
O-acetylhomoserine sulfhydrylase from Clostridium novyi. Cloning, expression of the gene and characterization of the enzyme	4
Effect of cell-permeable grouper Manganese Superoxide Dismutase on environmental stress in fish	4
Production and characterization of recombinant human leukemia inhibitory factor and evaluation of anti-fertility effects of rabbit anti-rhLIF in Balb/c mice	4
Expression, purification and characterization of the IcmG and IcmK proteins of the type IVB secretion system from Coxiella burnetii	4
Characterisation and comparative analysis of hydrophobin isolated from Pleurotus floridanus (PfH)	4
Metalation of a rice type 1 metallothionein isoform (OsMTI-1b)	4
A simple method to purify recombinant HCV core protein expressed in Pichia pastoris for obtaining virus-like particles and producing monoclonal antibodies	4
Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles	4
Comparison of physicochemical properties of recombinant buckwheat trypsin inhibitor (rBTI) and soybean trypsin inhibitor (SBTI)	4
Optimization of culture conditions for high-level expression of soluble and active tumor necrosis factor-α in E. coli	4
Expression of a β-glucosidase from Trichoderma reesei in Escherichia coli using a synthetic optimized gene and stability improvements by immobilization using magnetite nano-support	4
Development of a robust crystallization platform for immune receptor TREM2 using a crystallization chaperone strategy	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Bacterial sugar-binding protein as a one-step affinity purification tag on dextran-containing resins	4
Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
A scalable and reproducible manufacturing process for Phlebotomus papatasi salivary protein PpSP15, a vaccine candidate for leishmaniasis	4

Prediction of improved therapeutics for fabry disease patients generated by mutagenesis of the α-galactosidase A active site, dimer interface, and glycosylation region	4
High cell-density fermentation, expression and purification of bacteriophage lysin TSPphg, a thermostable antimicrobial protein from extremophilic Thermus bacteriophage TSP4	4
The expression, purification, and substrate analysis of matrix metalloproteinases in Drosophila melanogaster	4
Production of a human mitochondrial ABC transporter in E. coli	4
Expression and characterization of catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3	4
Bacterial expression and characterization of an anti-CD22 single-chain antibody fragment	4
One-step metal affinity purification of recombinant hFGF19 without using tags	4
High-level extracellular production and immobilisation of methyl parathion hydrolase from Plesiomonas sp. M6 expressed in Pichia pastoris	4