OOIR: Observatory of International Research

Papers

(The TQCC of Protein Expression and Purification is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-12-01 to 2025-12-01.)

Article	Citations
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	48
Protein inclusion into ice can dissociate subunits	47
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	42
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	28
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	23
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	19
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	19
Optimization of the expression of the main protease from SARS-CoV-2	18
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	18
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	17
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	17
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	17
Editorial Board	15
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	14
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	14
Editorial Board	13
Purification and characterization of the intrinsically disordered Arabidopsis thaliana protein SOG1	12
Simplified purification process of AsnB3 deamidated insulin variant and its comparison with human insulin	12
High performance production process development and scale-up of an anti-TSLP nanobody	12
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	11
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	11
Preparation and characterization of LGR5 LOOP region-specific nanobodies	11
Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	11
Purification of the HBV middle protein by two simple steps, application of ion exchange chromatography to improve protein homogeneity	11
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	11

Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	10
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	10
Editorial Board	10
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	10
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	9
Intracytoplasmic sperm injection-mediated strategy for the production of transgenic poultry as a bioreactor	9
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	9
Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	9
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	9
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	8
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose	8
Molecular chaperone GroEL-GroES enhances the soluble expression of biologically active ovine growth hormone in the prokaryotic system	8
Production of recombinant human ameloblastin by a fully native purification pathway	8
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	8
Novel (R)-Hydroxynitrile lyase enzyme of Pyrus communis: Purification and characterization of its physicochemical and kinetic properties	8
Strategies for rapid production of crystallization quality coatomer WD40 domains	8
Development of an effective method for purifying trypsin using a recombinant inhibitor	8
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	8
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	8
Editorial Board	8
Molecular cloning and functional analysis of a destabilase from Hirudinaria manillensis	8
One-step metal affinity purification of recombinant hFGF19 without using tags	8
An important consideration when expressing mAbs in Escherichia coli	7
Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli	7
Overproduction, purification, and transcriptional activity of recombinant Acinetobacter baylyi ADP1 RNA polymerase holoenzyme	7
Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR	7
Bacterial expression, purification and characterization of the human Dectin-1 lectin domain for structural and functional studies	7
Biochemical characterization of a novel bifunctional alginate lyase from Microbulbifer arenaceous	7
Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system	7
Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli	7
Expression, characterization and anti-colon cancer activity of recombinant ginseng peptides with amino acid tandem repeats	7
Enhanced performance of Thermococcus kodakarensis KOD1 polymerase in PCR via fusion to Sulfolobus tokodaii Sto7d	7
Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli	7
Development of a bispecific Nanobody anti-F17 fimbria as a potential therapeutic tool	6
Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions	6
Editorial Board	6
Purification and characterization of recombinant human superoxide dismutase integrated with resilin-like polypeptide	6
Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells	6
The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice	6
Editorial Board	6
Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds	6
Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations	6
Advantage of leaky expression, acid solubilization and CHAPS in the production of cost-effective bone morphogenetic Protein-2	6
Synthesis of BLF1-containing trimethyl chitosan nanoparticles and evaluation of its immunogenicity and protection in syrian mice by oral and subcutaneous injections	6
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli	6
Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant	6
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm	6
Soluble expression and purification of recombinant bovine ferritin H-chain	6
A multidomain PARP14 construct suitable for bacterial expression	6
A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing	6

Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum	6
Deepening in the understanding of the role of collagen subunits on the differential molecular arrangement of P. glauca and M. merluccius marine collagens	6
A protocol for production of perdeuterated OmpF porin for neutron crystallography	6
Purification and characterization of an asialofetuin specific lectin from the rhizome of Xanthosoma violaceum Schott	5
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	5
Affinity gel synthesis from the p-aminobenzoic acid derivative 4-amino-2-methylbenzoic acid and purification of polyphenol oxidase from various plant sources	5
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	5
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	5
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids	5
Efficient and economical purification platform for production of therapeutic nanobodies	5
Construction of a novel TROP2/CD3 bispecific antibody with potent antitumor activity and reduced induction of Th1 cytokines	5
Differentially labeled flaviviral protease-cofactor complex for NMR spectroscopic applications	5
High yield expression and purification of full-length Neurotensin with pyroglutamate modification	5
Expression of recombinant swine ferritin heavy chain with enhanced solubility in Escherichia coli and simplified purification of ferritin nanoparticles	5
Mini review for niche downstream processes	5
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	5
A procedure for producing an anti-AXL nanobody in E. coli	5
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	5
Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors	5
A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro	5
Enhanced production of stem bromelain in Pichia pastoris by coexpression of unfolded protein response activator gene HAC1P	5
High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system	5
Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	5
Navigating the Frontier: Advances in monoclonal antibody purity control	5
Editorial Board	5
Unlocking the potential of Extensin Signal peptide and Elastin-like polypeptide tag fused to Shigella dysenteriae's IpaDSTxB to improve protein expression and purification in Nicotiana tabacum and Med	5
CUREs for high-level Galectin-3 expression	5
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	5
Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli	5
A new combination approach to extracellular production of 5-aminolevulinic acid for purification and application in alleviating cadmium-induced oxidative stress in maize	5
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	5
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
Editorial Board	5
Expression, purification, characterization and crystallization of Panax quinquefolius ginsenoside glycosyltransferase Pq3-O-UGT2	5
Uncovering the role of leucine 59 in Renilla luciferase stability and activity with error-prone PCR: Implications for protein engineering	5
Preparation of recombinant neuritin protein	5
Corrigendum to “High-affinity nanobodies targeting IL-12B for the detection of fluorescence resonance energy transfer” [Protein Express. Purificat. 229 (2025) 106681]	5
Development of magnetic particle-based chemiluminescence immunoassay for quantitative determination of hepatitis C virus core antigen	5
Preparation and characterization of a novel humanized collagen III with repeated fragments of Gly300-Asp329	5
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease	4
High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP	4
Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells	4
Editorial Board	4
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography	4
Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli	4
Identification of host cell proteins that interact with the M protein of Pigeon paramyxovirus type 1	4
Bioinformatics analysis and polyclonal antibody preparation of Mesocricetus auratus DHX30	4
Time- and cost-efficient bacterial expression and purification of potato apyrase	4
A novel method for synthesizing authentic SARS-CoV-2 main protease	4
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Editorial Board	4
Fast IMAC purification of non-tagged S100A8/A9 (calprotectin) from Homo sapiens and Sus scrofa	4
Soluble expression of recombinant human interleukin-2 in Escherichia coli and its facile production	4
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	4
E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins	4
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense	4
A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration	4
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	4
Unravelling the reason for different binding behaviors exhibited by antibody aggregates towards preparative and analytical Protein A columns	4
Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens	4
Fusion with CTP increases the stability of recombinant neuritin	4
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	4
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination	4
Editorial Board	4
Expression, purification, and activity of sika deer prothymosin α protein	4
Functional expression of the chimera proteins of Nav1.7 and NavAb in Escherichia coli	4
Expression of a human Gb3/CD77 synthase in insect and human cells: comparison of activity and glycosylation	4
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4