Protein Expression and Purification

Article	Citations
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays	78
Recombinant expression of nanobodies and nanobody-derived immunoreagents	65
Expression and purification of recombinant G protein-coupled receptors: A review	38
Cloning, expression and characterization of a thermo-alkali-stable xylanase from Aspergillus oryzae LC1 in Escherichia coli BL21(DE3)	27
A new insight into purification of polyphenol oxidase and inhibition effect of curcumin and quercetin on potato polyphenol oxidase	25
Expression of SARS-CoV-2 surface glycoprotein fragment 319–640 in E. coli, and its refolding and purification	24
Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry	23
Removal of half antibody, hole-hole homodimer and aggregates during bispecific antibody purification using MMC ImpRes mixed-mode chromatography	23
Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays	22
Refolding, characterization, and dye decolorization ability of a highly thermostable laccase from Geobacillus sp. JS12	20
Expression, purification and characterization of a cold-adapted dextranase from marine bacteria and its ability to remove dental plaque	20
Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate	19
Production of membrane proteins in industry: The example of GPCRs	18
Enhancing soluble expression of sucrose phosphorylase in Escherichia coli by molecular chaperones	18
Stability and cytotoxicity of DPPH inhibitory peptides derived from biodegradation of chicken feather	17
Purification, biochemical characterization and DNA protection against oxidative damage of a novel recombinant superoxide dismutase from psychrophilic bacterium Halomonas sp. ANT108	16
Expression and purification of recombinant SARS-CoV-2 nucleocapsid protein in inclusion bodies and its application in serological detection	16
Skimmed milk as an alternative for IPTG in induction of recombinant protein expression	15
Purification and biochemical characteristics of a novel fructosyltransferase with a high FOS transfructosylation activity from Aspergillus oryzae S719	15
A potential downstream platform approach for WuXiBody-based IgG-like bispecific antibodies	15
Purification and characterization of a cold-adapted κ-carrageenase from Pseudoalteromonas sp. ZDY3	14
Linkers: A synergistic way for the synthesis of chimeric proteins	14
A review of alternative promoters for optimal recombinant protein expression in baculovirus-infected insect cells	13
The impact of a His-tag on DNA binding by RNA polymerase alpha-C-terminal domain from Helicobacter pylori	13
Enhancing the yield of human erythropoietin in Aspergillus niger by introns and CRISPR-Cas9	13

Soluble expression, purification, and secondary structure determination of human PDX1 transcription factor	13
Medium optimization for high yield production of human serum albumin in Pichia pastoris and its efficient purification	13
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli	13
Heterologous production of porcine derived antimicrobial peptide PR-39 in Escherichia coli using SUMO and intein fusion systems	13
Reconstitution and functional characterization of SARS-CoV-2 proofreading complex	12
Surface display of classical swine fever virus E2 glycoprotein on gram-positive enhancer matrix (GEM) particles via the SpyTag/SpyCatcher system	12
Affimers as an alternative to antibodies for protein biomarker enrichment	12
High-level expression and characterization of the thermostable leucine aminopeptidase Thelap from the thermophilic fungus Thermomyces lanuginosus in Aspergillus niger and its application in soy protei	12
Production of an invertebrate lysozyme of Scylla paramamosain in E.coli and evaluation of its antibacterial, antioxidant and anti-inflammatory effects	12
Expression and purification of the antimicrobial peptide Bin1b in Escherichia coli tagged with the fusion proteins CusF3H+ and SmbP	12
Bacterial overexpression and purification of soluble recombinant human serum albumin using maltose-binding protein and protein disulphide isomerase	12
Optimal secretion of thermostable Beta-glucosidase in Bacillus subtilis by signal peptide optimization	12
Purification and characterization of an inulinase produced by a Kluyveromyces marxianus strain isolated from blue agave bagasse	11
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	11
Recombinant production of active microbial transglutaminase in E. coli by using self-cleavable zymogen with mutated propeptide	11
Expression and purification of a novel single-chain diabody (scDb-hERG1/β1) from Pichia pastoris transformants	10
A lectin with anti-microbial and anti proliferative activities from Lantana camara, a medicinal plant	10
In vitro preparation of uniform and nucleic acid free hepatitis B core particles through an optimized disassembly-purification-reassembly process	10
Expression and purification of S5196-272 and S6200-317 proteins from Tilapia Lake Virus (TiLV) and their potential use as vaccines	10
Expression and characterization of l-arabinose isomerase from Geobacillus stearothermophilus for improved activity under acidic condition	10
Removing half antibody byproduct by Protein A chromatography during the purification of a bispecific antibody	10
Expression and characterization of a chitinase from Serratia marcescens	10
Large scale production of synthetic spider silk proteins in Escherichia coli	10
Non-chromatographic purification of thermostable endoglucanase from Thermotoga maritima by fusion with a hydrophobic elastin-like polypeptide	10
Cloning, expression and purification of the low-complexity region of RanBP9 protein	10
Enzymatic properties of an efficient glucan branching enzyme and its potential application in starch modification	10
Purification and anticancer activity of glutaminase and urease free intracellular l-asparaginase from Chaetomium sp.	9
Characterization of a bifunctional and endolytic alginate lyase from Microbulbifer sp. ALW1 and its application in alginate oligosaccharides production from Laminaria japonica	9
Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody	9
Cloning and characterization of the Bambusa oldhamii BoMDH-encoded malate dehydrogenase	9
Development of a monoclonal antibody against canine parvovirus NS1 protein and investigation of NS1 dynamics and localization in CPV-infected cells	9
Refolded recombinant major capsid protein (MCP) from Infectious Spleen and Kidney Necrosis Virus (ISKNV) effectively stimulates serum specific antibody and immune related genes response in Nile tilapi	9
Structural and functional characterizations and heterogenous expression of the antimicrobial peptides, Hidefensins, from black soldier fly, Hermetia illucens (L.)	9
An improved method for the expression and purification of porcine dihydropyrimidine dehydrogenase	8
Improving the yield of recalcitrant Nanobodies® by simple modifications to the standard protocol	8
The structure and properties of MFG-E8 and the In vitro assessment of its toxic effects on myoblast cells	8
Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	8
Separating antibody species containing one and two kappa light chain constant region by KappaSelect affinity chromatography	8
A novel recombinant expression and purification approach for the full-length anti-apoptotic membrane protein Bcl-2	8
Simultaneously targeting nitrocellulose and antibody by a dual-headed protein	8
Expression, purification, and characterization of recombinant apoPholasin	8
Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch	8
Protein L chromatography: A useful tool for monitoring/separating homodimers during the purification of IgG-like asymmetric bispecific antibodies	8
Nanobody production can be simplified by direct secretion from Escherichia coli	8
Starch-degrading enzymes from the brown-rot fungus Fomitopsis palustris	8
Characterization of an acidic pectin methylesterase from Paenibacillus xylanexedens and its application in fruit processing	8
Modified chemical method for efficient transformation and diagnosis in Pichia pastoris	8
Preparation of rabbit polyclonal antibody against porcine gasdermin D protein and determination of the expression of gasdermin D in cultured cells and porcine tissues	7
Recombinant production of Trx-Ib-AMP4 and Trx-E50-52 antimicrobial peptides and antimicrobial synergistic assessment on the treatment of methicillin-resistant Staphylococcus aureus under in vitro and	7
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	7

Novel strategy for expression and characterization of rabies virus glycoprotein	7
Removing light chain-missing byproducts and aggregates by Capto MMC ImpRes mixed-mode chromatography during the purification of two WuXiBody-based bispecific antibodies	7
Improving the heterologous expression of human β-defensin 2 (HBD2) using an experimental design	7
Expression and purification of codon-optimized cre recombinase in E. coli	7
Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	7
Characterization of a single chain variable fragment of nivolumab that targets PD-1 and blocks PD-L1 binding	7
Optimization strategies for expression of a novel bifunctional anti-PD-L1/TGFBR2-ECD fusion protein	7
Molecular cloning, characterization, and heterologous expression of an acetyl-CoA acetyl transferase gene from Sanghuangporus baumii	7
Biochemical characterization and detection of antitumor activity of l-asparaginase from thermophilic Geobacillus kaustophilus DSM 7263T	7
Application of pH-salt dual gradient elution in purifying a WuXiBody-based bispecific antibody by MMC ImpRes mixed-mode chromatography	7
Development of a biochemical and biophysical suite for integral membrane protein targets: A review	7
Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets	7
Design, expression, purification and crystallization of human 14-3-3ζ protein chimera with phosphopeptide from proapoptotic protein BAD	6
Characterization of the Fc–III–4C-based recombinant protein expression system by using carbonic anhydrase as the model protein	6
Purification of native CCL7 and its functional interaction with selected chemokine receptors	6
Recombinant protein production and purification of SiiD, SiiE and SiiF - Components of the SPI4-encoded type I secretion system from Salmonella Typhimurium	6
Cell-free expression of natively folded hydrophobins	6
Effect of Clp protease from Corynebacterium glutamicum on heterologous protein expression	6
Method for efficient soluble expression and purification of recombinant human interleukin-15	6
Highly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro encapsulation of ruthenium nanoparticles for catalysis	6
PiP2 favors an α-helical structure of non-recombinant Hsp12 of Saccharomyces cerevisiae	6
The effects of L-arginine on protein stability and DNA binding ability of SaeR, a transcription factor in Staphylococcus aureus	6
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	6
Optimized expression of classical swine fever virus E2 protein via combined strategy in Pichia pastoris	6
Affinity purification of Car9-tagged proteins on silica-derivatized spin columns and 96-well plates	6
Expression, purification and crystallization of CLK1 kinase – A potential target for antiviral therapy	6
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	6
Production of bioactive human IFN-γ protein by agroinfiltration in tobacco	6
Heterologous expression of cobalamin dependent class-III enzymes	6
Improvements of a high-throughput protein purification process using a calcium-dependent setup	6
Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata	6
Expression and functional identification of two homologous nicotine dehydrogenases, NicA2 and Nox, from Pseudomonas sp. JY-Q	6
A thermostable glucose oxidase from Aspergillus heteromophus CBS 117.55 with broad pH stability and digestive enzyme resistance	6
Expression, purification and characterization of the RhoA-binding domain of human SHIP2 in E.coli	5
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	5
Optimizing purification of the peripheral membrane protein FAM92A1 fused to a modified spidroin tag	5
cDNA cloning, prokaryotic expression, and functional analysis of squalene synthase (SQS) in Camellia vietnamensis Huang	5
Bioconversion of recombinantly produced precursor peptide pqqA into pyrroloquinoline quinone (PQQ) using a cell-free in vitro system	5
Enhanced expression and purification of nucleotide-specific ribonucleases MC1 and Cusativin	5
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	5
Characterization of a hyperphosphorylated variant of G protein-coupled receptor kinase 5 expressed in E. coli	5
Removing a light-chain missing byproduct by MMC ImpRes mixed-mode chromatography under weak partitioning mode in purifying a WuXiBody-based bispecific antibody	5
A generalizable protocol for expression and purification of membrane-bound bacterial phosphoglycosyl transferases in liponanoparticles	5
Removing a difficult-to-separate byproduct by Capto L affinity chromatography during the purification of a WuXiBody-based bispecific antibody	5
N-terminal fusion of the N-terminal domain of bacterial enzyme I facilitates recombinant expression and purification of the human RNA demethylases FTO and Alkbh5	5
Expression, purification, and structural analysis of the full-length human integral membrane protein γ-sarcoglycan	5
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation	5
Recombinant expression of the precursor of rat lung surfactant protein B in Escherichia coli and its antibacterial mechanism	5
Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation	5
Affinity tag effect on the salt rejection potential of Halomonas elongata aquaporin incorporated in thin film nanocomposite membrane	5
An efficient system to generate truncated human angiotensin converting enzyme 2 (hACE2) capable of binding RBD and spike protein of SARS-CoV2	5
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	5
Retinol binding protein IV purified from Escherichia coli using intein-mediated cleavage as a suitable replacement for serum sources	5
Peptidyl-prolyl isomerase and the biological activities of recombinant protein cyclophilin from Pyropia yezoensis (PyCyp)	5
Expression, purification, and characterization of human mannose-6-phosphate receptor – Extra cellular domain from a stable cell line utilizing a small molecule biomimetic of the mannose-6-phosphate mo	5
Expression and characterization of a thermotolerant and pH-stable hyaluronate lyase from Thermasporomyces composti DSM22891	5
Cell free protein synthesis versus yeast expression – A comparison using insulin as a model protein	5
Pseudomonas aeruginosa recombinant L-asparaginase: Large scale production, purification, and cytotoxicity on THP-1, MDA-MB-231, A549, Caco2 and HCT-116 cell lines	5
Facile expression and purification of active human growth hormone in E. coli by a cleavable self-aggregating tag scheme	5
Comparison of three palm tree peroxidases expressed by Escherichia coli: Uniqueness of African oil palm peroxidase	5
Identification and characterization of a hydrophobin Vmh3 from Pleurotus ostreatus	5
Addition of arginine hydrochloride and proline to the culture medium enhances recombinant protein expression in Brevibacillus choshinensis: The case of RBD of SARS-CoV-2 spike protein and its antibody	5
Efficient improvement of surface displayed lipase from Rhizomucor miehei in PichiaPink™ protease-deficient system	5
Expression and purification of a recombinant ELRL-MAP30 with dual-targeting anti-tumor bioactivity	4
Reconstitution of the full transmembrane cadherin-catenin complex	4
Improving SDS-PAGE method for monoclonal antibodies: The advantages of Tris-Acetate over Tris-Glycine SDS-PAGE system and comparison with CE-SDS method	4
A scalable and reproducible manufacturing process for Phlebotomus papatasi salivary protein PpSP15, a vaccine candidate for leishmaniasis	4
Optimization of culture conditions for high-level expression of soluble and active tumor necrosis factor-α in E. coli	4
Cloning, characterization and expression analysis of glutathione S-transferase from the Antarctic yeast Rhodotorula mucilaginosa AN5	4
Effect of cell-permeable grouper Manganese Superoxide Dismutase on environmental stress in fish	4
Development of a robust crystallization platform for immune receptor TREM2 using a crystallization chaperone strategy	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Expression, purification and characterization of the IcmG and IcmK proteins of the type IVB secretion system from Coxiella burnetii	4
Functional production, characterization, and immobilization of a cold-adapted cutinase from Antarctic Rhodococcus sp.	4
High-level extracellular production and immobilisation of methyl parathion hydrolase from Plesiomonas sp. M6 expressed in Pichia pastoris	4
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles	4
Comparison of physicochemical properties of recombinant buckwheat trypsin inhibitor (rBTI) and soybean trypsin inhibitor (SBTI)	4
A simple method to purify recombinant HCV core protein expressed in Pichia pastoris for obtaining virus-like particles and producing monoclonal antibodies	4
Two-step purification of tag-free norovirus-like particles from silkworm larvae (Bombyx mori)	4
Immobilization of fibrinolytic protease from Mucor subtilissimus UCP 1262 in magnetic nanoparticles	4

O-acetylhomoserine sulfhydrylase from Clostridium novyi. Cloning, expression of the gene and characterization of the enzyme	4
Expression of a β-glucosidase from Trichoderma reesei in Escherichia coli using a synthetic optimized gene and stability improvements by immobilization using magnetite nano-support	4
Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase	4
Expression, purification and crystallization of TGW6, which limits grain weight in rice	4
Bacterial expression and characterization of an anti-CD22 single-chain antibody fragment	4
Bacterial sugar-binding protein as a one-step affinity purification tag on dextran-containing resins	4
Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2	4
Metalation of a rice type 1 metallothionein isoform (OsMTI-1b)	4
Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative impact	4
Prediction of improved therapeutics for fabry disease patients generated by mutagenesis of the α-galactosidase A active site, dimer interface, and glycosylation region	4
Biochemical characterization and redesign of the coenzyme specificity of a novel monofunctional NAD+-dependent homoserine dehydrogenase from the human pathogen Neisseria gonorrhoeae	4
High cell-density fermentation, expression and purification of bacteriophage lysin TSPphg, a thermostable antimicrobial protein from extremophilic Thermus bacteriophage TSP4	4
Expression and purification of recombinant mouse CRISP4 using a baculovirus system	4
Production of a human mitochondrial ABC transporter in E. coli	4
A custom ÄKTA avant configuration enabling automated parallel protein purification over a range of process scales	4
Production and characterization of recombinant human leukemia inhibitory factor and evaluation of anti-fertility effects of rabbit anti-rhLIF in Balb/c mice	4
A comparison between MBP- and NT* as N-terminal fusion partner for recombinant protein production in E. coli	4
Characterisation and comparative analysis of hydrophobin isolated from Pleurotus floridanus (PfH)	4
Viral removal by column chromatography in downstream processing of monoclonal antibodies	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
Expression and characterization of catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3	3
One-step metal affinity purification of recombinant hFGF19 without using tags	3
Adaptation process of engineered cell line FCHO/IL-24 stably secreted rhIL-24 in serum-free suspension culture	3
Non-structural protein 1 from Japanese encephalitis virus expressed in E. coli retains its molecular weight and immunogenicity	3
Affinity purification of human alpha galactosidase utilizing a novel small molecule biomimetic of alpha-D-galactose	3
Production of functional recombinant prolyl hydroxylase-2 enzyme in insect cells for small molecule inhibitor screening studies	3
MmpL3, the trehalose monomycolate transporter, is stable in solution in several detergents and can be reconstituted into peptidiscs	3
Selection, preparation and characterization of scFv against human lipocalin 6 by phage display technology	3
Optimized production strategy of the major capsid protein HPV 16L1 non-assembly variant in E. coli	3
Immunization of rabbits with recombinant Clostridium perfringens alpha toxins CPA-C and CTB-CPA-C in a bicistronic design expression system confers strong protection against challenge	3
Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies	3
The expression, purification, and substrate analysis of matrix metalloproteinases in Drosophila melanogaster	3
Purification and preliminary characterization of four Rel homologues from pathogenic bacteria: Implications for species-specific inhibitor design	3
Production and characterization of polyclonal and monoclonal antibodies of lamprey pore-forming protein	3
Effects of removing a highly conserved disulfide bond in ubiquitin-associated domain of human HOIP on biochemical characteristics	3
Expression of rice β-exoglucanase II (OsExoII) in Escherichia coli, purification, and characterization	3
Msx1 cooperates with Runx1 for inhibiting myoblast differentiation	3
Capture and purification of an untagged nanobody by mixed weak cation chromatography and cation exchange chromatography	3
Comparing the relative robustness of byproduct removal by wash and by elution in column chromatography for the purification of a bispecific antibody	3
Antibody aggregation can lead to reduced Protein A binding capacity and low step yield	3
Co-expression of Pseudomonas alcaligenes lipase and its specific foldase in Pichia pastoris by a dual expression cassette strategy	3
Expression and purification of soluble recombinant SapM from Mycobacterium tuberculosis	3
High-yield production in E. coli and characterization of full-length functional p13II protein from human T-cell leukemia virus type 1	3
Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2	3
Production of active recombinant human aldehyde oxidase (AOX) in the baculovirus expression vector system (BEVS) and deployment in a pre-clinical fraction-of-control AOX compound exposure assay	3
Improved thermostability of D-allulose 3-epimerase from Clostridium bolteae ATCC BAA-613 by proline residue substitution	3
Production of selenomethionine labeled polyglycine hydrolases in Pichia pastoris	3
Antibody mimetic drug conjugate manufactured by high-yield Escherichia coli expression and non-covalent binding system	3
Identification of catalytically distinct arylalkylamine N-acetyltransferase splicoforms from Tribolium castaneum	3
Chemometric modeling of the electrochemical data to investigate proline cis/trans isomeration effect on aggregation of Tau protein	3
Cloning, expression, and characterization of a novel plant type cryptochrome gene from the green alga Haematococcus pluvialis	3
Large scale purification and characterization of A21 deamidated variant-most prominent post translational modification (PTM) for insulins which is also widely observed in insulins pharmaceutical manuf	3
Expression in Escherichia coli, purification and kinetic characterization of LAPLm, a Leishmania major M17-aminopeptidase	3
Expression of HCV genotype-4 core antigen in prokaryotic E. coli system for diagnosis of HCV infection in Egypt	3
Heterologous gene expression and characterization of recombinant aspartate aminotransferase from Geobacillus thermopakistaniensis	3
A high yielding IFNAR1 ECD mammalian expression process for use in autoimmune disease drug development	3
Robust and facile purification of full-length, untagged human Nedd4 as a recombinant protein from Escherichia coli	3
Expression, purification and identification of isotope-labeled recombinant cystatin C protein in Escheichia coli intended for absolute quantification using isotope dilution mass spectrometry	3
Co-expression of recombinant human collagen α1(III) chain with viral prolyl 4-hydroxylase in Pichia pastoris GS115	3
Heterologous expression and characterisation of a keratinase produced by Chryseobacterium carnipullorum	3
Structural exploration of Y-domain reveals its essentiality in HEV pathogenesis	3
Heteroexpression and biochemical characterization of thermostable citrate synthase from the cyanobacteria Anabaena sp. PCC7120	3
Identification of chicken-derived scFv against N-glycolylneuraminic acid retrieved from an immune library by phage display	3
Enhancing the thermostability of transglutaminase from Streptomyces mobaraensis based on the rational design of a disulfide bond	3
Expression, purification and preliminary characterisation of the choline transporter LicB from opportunistic bacterial pathogens	3
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	3
Recombinant expression and characterization of Oryctolagus cuniculus chymosin in Komagataella phaffii (Pichia pastoris)	3
A simplified method for producing laboratory grade recombinant TEV protease from E. coli	3
Expression, purification, and refolding of diverse class IB hydrophobins	3
How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger	3
Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein	2
Refolding and purification of cGMP-grade recombinant human neurturin from Escherichia coli inclusion bodies	2
Purification, crystallization, and X-ray diffraction analysis of myocyte enhancer factor 2D and DNA complex	2
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites	2
Recombinant highly antigenic truncated fusion-based protein as a diagnostic antigen for anti-SARS-CoV-2 nucleocapsid antibody ELISA	2
A comparative study and evaluation of anti-EGFR nanobodies expressed in Pichia pastoris and Escherichia coli as antitumor moieties	2
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	2
Expression, purification, and study on the efficiency of a new potent recombinant scFv antibody against the SARS-CoV-2 spike RBD in E. coli BL21	2
Optimization of the expression of the main protease from SARS-CoV-2	2
Purification and HDL-like particle formation of apolipoprotein A-I after co-expression with the EDDIE mutant of Npro autoprotease	2
New method for immobilising diverse proteins onto cubic micro-protein polyhedrin crystals	2
Molecular cloning and biochemical characterization of a trehalose synthase from Myxococcus sp. strain V11	2
Identification and characterization of Poly(ADP-ribose) polymerase-1 interacting proteins during development of Dictyostelium discoideum	2
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	2
Expression and purification of the human tumor suppressor protein RNASET2 in CHO–S cells	2
Tick-borne encephalitis nonstructural protein NS1 expressed in E. coli retains immunological properties of the native protein	2
NADP+-dependent l-arginine dehydrogenase from Pseudomonas velonii: Purification, characterization and application to an l-arginine assay	2
Screening of six cation exchange resins for high binding capacity, monomer purity and step yield: A case study	2
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	2
Efficient matrix-assisted refolding of the recombinant anti-staphylococcal truncated endolysin LysKCA and its structural and enzymatic description	2
Characterization of guanine nucleotide exchange activity of DH domain of human FGD2	2
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	2
Tandem fusion of albumin-binding domains promoted soluble expression and stability of recombinant trichosanthin in vitro and in vivo	2
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)	2
Rational design of a Nivolumab-based ANTI-PD-1 single chain variable fragment that blocks the interaction between PD-1 expressed on T-CELLS and PD-L1 ON CHO cells	2