Protein Expression and Purification

Article	Citations
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	38
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	37
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	32
Generation and characterization of a humanized anti-IL-17A rabbit monoclonal antibody	26
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	23
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	21
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	21
Protein inclusion into ice can dissociate subunits	20
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	20
Optimization of the expression of the main protease from SARS-CoV-2	18
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	18
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	16
Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method	16
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	16
High performance production process development and scale-up of an anti-TSLP nanobody	16
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	16
Editorial Board	15
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	15
Editorial Board	15
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	14
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	14
Purification and characterization of the intrinsically disordered Arabidopsis thaliana protein SOG1	13
Polycistronic baculovirus expression of SUGT1 enables high-yield production of recombinant leucine-rich repeat proteins and protein complexes	13
Preparation and characterization of LGR5 LOOP region-specific nanobodies	13
Simplified purification process of AsnB3 deamidated insulin variant and its comparison with human insulin	12

Separation and identification of ACE inhibitory peptides from lizard fish proteins hydrolysates by metal affinity-immobilized magnetic liposome	11
Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	11
The periplasmic expression and purification of AA15 lytic polysaccharide monooxygenases from insect species in Escherichia coli	10
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	10
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	10
Purification and characterization of an FeII- and α-ketoglutarate-dependent xanthine hydroxylase from Aspergillus oryzae	10
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	10
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	10
Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2	10
Improvement of thermostability of cholesterol oxidase from streptomyces Sp. SA-COO by random mutagenesis	10
Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	9
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	9
Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata	8
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose	8
High-level extracellular production and immobilisation of methyl parathion hydrolase from Plesiomonas sp. M6 expressed in Pichia pastoris	8
Editorial Board	8
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	8
Molecular chaperone GroEL-GroES enhances the soluble expression of biologically active ovine growth hormone in the prokaryotic system	8
Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli	8
One-step metal affinity purification of recombinant hFGF19 without using tags	8
Production of recombinant human ameloblastin by a fully native purification pathway	8
Strategies for rapid production of crystallization quality coatomer WD40 domains	8
Development of an effective method for purifying trypsin using a recombinant inhibitor	8
Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations	7
A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing	7
Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system	7
Expression, characterization and anti-colon cancer activity of recombinant ginseng peptides with amino acid tandem repeats	7
Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant	7
Advantage of leaky expression, acid solubilization and CHAPS in the production of cost-effective bone morphogenetic Protein-2	7
Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli	7
An important consideration when expressing mAbs in Escherichia coli	7
Biochemical characterization of a novel bifunctional alginate lyase from Microbulbifer arenaceous	7
Bacterial expression, purification and characterization of the human Dectin-1 lectin domain for structural and functional studies	7
Synthesis of BLF1-containing trimethyl chitosan nanoparticles and evaluation of its immunogenicity and protection in syrian mice by oral and subcutaneous injections	7
Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli	7
Overproduction, purification, and transcriptional activity of recombinant Acinetobacter baylyi ADP1 RNA polymerase holoenzyme	7
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum	7
Purification and characterization of recombinant human superoxide dismutase integrated with resilin-like polypeptide	7
Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR	7
Editorial Board	7
Editorial Board	6
Deepening in the understanding of the role of collagen subunits on the differential molecular arrangement of P. glauca and M. merluccius marine collagens	6
A protocol for production of perdeuterated OmpF porin for neutron crystallography	6
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli	6
Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	6
The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice	6
Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds	6
Unlocking the potential of Extensin Signal peptide and Elastin-like polypeptide tag fused to Shigella dysenteriae's IpaDSTxB to improve protein expression and purification in Nicotiana tabacum and Med	6
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm	6
Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells	6

High yield expression and purification of full-length Neurotensin with pyroglutamate modification	6
Expression and purification of a novel single-chain diabody (scDb-hERG1/β1) from Pichia pastoris transformants	6
Development of a bispecific Nanobody anti-F17 fimbria as a potential therapeutic tool	6
A multidomain PARP14 construct suitable for bacterial expression	6
Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions	6
High level expression and purification of recombinant 3ABC non-structural protein of foot-and-mouth disease virus using SUMO fusion system	5
Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids	5
Combining the advantages of prokaryotic expression and T7 phage display systems to obtain antigens for antibody preparation	5
Design, synthesis and application of near-infrared fluorescence probe IR-780-Crizotinib in detection of ALK positive tumors	5
Preparation of recombinant neuritin protein	5
Characterization of two 3-deoxy-d-Arabino-Heptulosonate 7-phosphate synthases from Bacillus methanolicus	5
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	5
Editorial Board	5
Expression, purification and microscopic characterization of human ATP-binding cassette sub-family B member 7 protein	5
Editorial Board	5
Differentially labeled flaviviral protease-cofactor complex for NMR spectroscopic applications	5
Expression, purification, characterization and crystallization of Panax quinquefolius ginsenoside glycosyltransferase Pq3-O-UGT2	5
Preparation and characterization of a novel humanized collagen III with repeated fragments of Gly300-Asp329	5
Affinity gel synthesis from the p-aminobenzoic acid derivative 4-amino-2-methylbenzoic acid and purification of polyphenol oxidase from various plant sources	5
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	5
Uncovering the role of leucine 59 in Renilla luciferase stability and activity with error-prone PCR: Implications for protein engineering	5
Construction of a novel TROP2/CD3 bispecific antibody with potent antitumor activity and reduced induction of Th1 cytokines	5
Soluble expression and purification of recombinant bovine ferritin H-chain	5
Purification and characterization of an asialofetuin specific lectin from the rhizome of Xanthosoma violaceum Schott	5
Corrigendum to “High-affinity nanobodies targeting IL-12B for the detection of fluorescence resonance energy transfer” [Protein Express. Purificat. 229 (2025) 106681]	5
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
Recombinant organophosphorus hydrolase (OPH) expression in E. coli for the effective detection of organophosphate pesticides	5
CUREs for high-level Galectin-3 expression	5
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	5
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination	4
Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2	4
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	4
IgG-like bispecific antibody platforms with built-in purification-facilitating elements	4
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	4
A procedure for producing an anti-AXL nanobody in E. coli	4
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	4
Functional expression of the chimera proteins of Nav1.7 and NavAb in Escherichia coli	4
Optimization strategies for expression and purification of soluble N-terminal domain of human centriolar protein SAS-6 in Escherichia coli	4
Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens	4
Soluble expression of recombinant human interleukin-2 in Escherichia coli and its facile production	4
A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration	4
E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)	4
Time- and cost-efficient bacterial expression and purification of potato apyrase	4
Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system	4
A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro	4
Unravelling the reason for different binding behaviors exhibited by antibody aggregates towards preparative and analytical Protein A columns	4
Purification and characterization of an amyloidogenic repeat domain from the functional amyloid Pmel17	4
Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation	4
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	4
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease	4
Identification of host cell proteins that interact with the M protein of Pigeon paramyxovirus type 1	4
A novel method for synthesizing authentic SARS-CoV-2 main protease	4
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	4
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	4
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense	4
Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli	4
Fusion with CTP increases the stability of recombinant neuritin	4
High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP	4
Use of the redox-dependent intein system for enhancing production of the cyclic green fluorescent protein	3
Column-free purification of an artificial protein nanocage, TIP60	3
Expression and characterization of a thermotolerant and pH-stable hyaluronate lyase from Thermasporomyces composti DSM22891	3
Method for efficient soluble expression and purification of recombinant hyperactive Tn5 transposase	3
Generation and characterization of a monoclonal antibody against FGFR3 that protects mice from BoNT/A	3
Identification of chicken-derived scFv against N-glycolylneuraminic acid retrieved from an immune library by phage display	3
Fast IMAC purification of non-tagged S100A8/A9 (calprotectin) from Homo sapiens and Sus scrofa	3
Recombinant expression, purification, and characterization of full-length human BST-2 from Escherichia coli	3
Production of enzymes for the removal of odorous substances in plant biomass	3
Biochemical and structural characterization of a novel L-isoleucine-4-dioxygenase (RaIDO) from Rahnella aquatilis	3
Heterologous expression of a novel galactose-1-phosphate uridylyltransferase from Thermodesulfatator indicus and its application for bioproduction of Gal-β-1,4-GlcNAc-X	3
Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody	3
StxB fusion strategy for immunogenic enhancement: Recombinant expression of AcAMP antimicrobial peptide	3
Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis	3
Enhancing the expression of multi-antigen chimeric TGAGS/BST protein from Toxoplasma gondii in Escherichia coli BL 21 Star during batch cultivation	3
Purification and anticancer activity of glutaminase and urease free intracellular l-asparaginase from Chaetomium sp.	3

Prokaryotic expression of soluble IFN-λ1 recombinant protein with cold-shock system	3
Isolation, purification and characterization of a novel esterase from camel rumen metagenome	3
Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells	3
Optimization of the heterologous expression and purification of Plasmodium falciparum generative cell specific 1 in Escherichia coli	3
Editorial Board	3
Expression, purification and structural characterization of resveratrol synthase from Polygonum cuspidatum	3
Editorial Board	3
Design and construction of a phage-displayed Camelid nanobody library using a simple bioinformatics method	3
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica	3
Enhanced expression and solubility of main protease (Mpro) of SARS-CoV-2 from E. coli	3
Enzymatic characterization and thermostability improvement of an acidophilic endoxylanase PphXyn11 from Paenibacillus physcomitrellae XB	3
Editorial Board	3
Expression and Characterization of the Staphylococcus aureus RecA protein: A mapping of canonical functions	3
The combined effect of the gene copy number and chaperone overexpression on the recombinant bovine chymosin production in Pichia pastoris, with mutant ADH2 promoter	3
Biochemical characterization and antifungal activity of a recombinant β-1,3-glucanase FlGluA from Flavobacterium sp. NAU1659	3
Bioindustrial applications of thermostable Endoglucanase purified from Trichoderma viride towards the conversion of agrowastes to value-added products	3
Characterization of recombinant E. coli expressing a novel fucosidase from Bacillus cereus 2–8 belonging to GH95 family	3
Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells	3
Production of recombinant human proliferating cellular nuclear antigen (PCNA) for structural and biophysical characterization	3
Cloning and characterization of a hyaluronate lyase EsHyl8 from Escherichia sp. A99	3
Efficient development of nanobody-based affinity chromatography for AAV8 purification	3
High level stable expression of recombinant HIV gp120 in glutamine synthetase gene deficient HEK293T cells	3
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites	3
Editorial Board	3
Expression and characterization of a novel β-1,4-endoglucanase from Bacillus subtilis strain isolated from a pulp and paper mill wastewater	3
Molecular cloning, expression, and functional analysis of copper amine oxidase gene from mulberry (Morus alba L.)	3
Expression, purification and preliminary crystallographic analysis of bacterial transmembrane protein EfeU for iron import	3
Generation of a blockage monoclonal antibody of LILRB1 against HLA-G	2
Improved self-cleaving precipitation tags for efficient column free bioseparations	2
An improved production and purification protocol for recombinant soluble human fibroblast activation protein alpha	2
Optimized extraction, identification and characterization of the mosquitocidal surface layer protein from a local bacterial isolate Lysinibacillus sphaericus Q001	2
Overcoming Fluorescence Loss in mEOS-based AAA+ Unfoldase Reporters Through Covalent Linkage	2
A two-step method preparation of semaglutide through solid-phase synthesis and inclusion body expression	2
Generation and characterization of a monoclonal antibody that binds to Galectin-1	2
Functional expression and purification of recombinant full-length human ATG7 protein with HIV-1 Tat peptide in Escherichia coli	2
Editorial Board	2
Thermostable phenylacetic acid degradation protein TtPaaI from Thermus thermophilus as a scaffold for tetravalent display of proteins	2
Effects of removing a highly conserved disulfide bond in ubiquitin-associated domain of human HOIP on biochemical characteristics	2
Recombinant production of Trx-Ib-AMP4 and Trx-E50-52 antimicrobial peptides and antimicrobial synergistic assessment on the treatment of methicillin-resistant Staphylococcus aureus under in vitro and	2
Editorial Board	2
Evaluate the potential use of TonB-dependent receptor protein as a subunit vaccine against Aeromonas veronii infection in Nile tilapia (Oreochromis niloticus)	2
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles	2
Editorial Board	2
Editorial Board	2
Heterologous expression and biochemical comparison of two homologous SoxX proteins of endosymbiotic Candidatus Vesicomyosocius okutanii and free-living Hydrogenovibrio crunogenus from deep-sea environ	2
Trehalose-6-phosphate phosphatase expression and enzymatic properties of Fusarium graminearum	2
Enhanced expression and purification of nucleotide-specific ribonucleases MC1 and Cusativin	2
Expression and purification of human interferon alpha 2a (IFNα2a) in the methylotrophic yeast Pichia pastoris	2
Monitoring of the disulfide scrambled species by mixed-mode SEC-HPLC during the purification of a bispecific antibody	2
Overexpression and truncation of a novel cold-adapted lipase with improved enzymatic characteristics	2
Characterization of a bifunctional and endolytic alginate lyase from Microbulbifer sp. ALW1 and its application in alginate oligosaccharides production from Laminaria japonica	2
Recombinant expression, purification, and structural analysis of two ectodomains of Syndecan-1	2
Functional characterization and structural insights of three cutinases from the ascomycete Fusarium verticillioides	2
Characterisation and comparative analysis of hydrophobin isolated from Pleurotus floridanus (PfH)	2
Comparing the relative robustness of anion exchange and the corresponding mixed-mode chromatography on removing a weakly-bound byproduct: A case study of bispecific antibody purification	2
Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	2
Resin and loading condition screening for effective and robust byproducts removal by anion exchange chromatography: A case study	2
Preparation and characterization of a high-affinity monoclonal antibody against nerve growth factor	2
Immunoglobulin-binding protein-based affinity chromatography in bispecific antibody purification: Functions beyond product capture	2
Efficient production of recombinant human FVII in CHO cells using the piggyBac transposon system	2
Recombinant expression and functional characterization of FadD2 protein in Mycobacterium tuberculosis	2
Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33	2
Editorial Board	2
Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies	2
Cell-free protein production of a gamma secretase homolog	2
Recombinant production of antibody antigen-binding fragments with an N-terminal human growth hormone tag in mammalian cells	2
Cloning and characterization of a novel nitric oxide synthase gene from Exiguobacterium profundum and its expression in Escherichia coli	2
Recombinant highly antigenic truncated fusion-based protein as a diagnostic antigen for anti-SARS-CoV-2 nucleocapsid antibody ELISA	2
Expression, purification and crystallization of TGW6, which limits grain weight in rice	2
Cloning and characterization of a novel DNase gene from Trichogramma pretiosum	2
Biochemical characterization of LysVpKK5 endolysin from a marine vibriophage	2
Corrigendum to “Expression and characterization of a novel β-1,4-endoglucanase from Bacillus subtilis strain isolated from a pulp and paper mill wastewater” [J. Protein Expr. Purif., 220 (2024) 106490	2
Heterologous expression, purification and single step efficient refolding of recombinant tissue plasminogen activator (Reteplase) from E. coli	2
Tetrahymena thermophila granule lattice protein 3 improves solubility of sexual stage malaria antigens expressed in Escherichia coli	2
STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins	2
Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy	2
Cell-free protein synthesis of CD1E and B2M protein and in vitro interaction	2
CHO cathepsin B identified as the protease responsible for a target bispecific antibody fragmentation	2
Viral removal by column chromatography in downstream processing of monoclonal antibodies	2
Recombinant expression and surface display of a zearalenone lactonohydrolase from Trichoderma aggressivum in Escherichia coli	2
Editorial Board	2
Proteomic analysis reveals a role of ADP-glucose pyrophosphorylase in the asynchronous filling of rice superior and inferior spikelets	2
Identification and characterization of a hydrophobin Vmh3 from Pleurotus ostreatus	2
Expression in Escherichia coli, purification and kinetic characterization of LAPLm, a Leishmania major M17-aminopeptidase	2
Expression and purification of the NG domain from human SRα, a key component of the Signal Recognition Particle (SRP) receptor	1
MAM7 from Vibrio parahaemolyticus: Expression, purification and effects on RAW264.7 cells	1
Improved yield of recombinant human IFN-α2b from mammalian cells using heterologous signal peptide approach	1
An efficient system to generate truncated human angiotensin converting enzyme 2 (hACE2) capable of binding RBD and spike protein of SARS-CoV2	1
Expression, solubility monitoring, and purification of the co-folded LUBAC LTM domain by structure-guided tandem folding in autoinducing cultures	1
Recombinant expression of Yersinia ruckeri outer membrane proteins in Escherichia coli extracellular vesicles	1
Capture and purification of an untagged nanobody by mixed weak cation chromatography and cation exchange chromatography	1
Functional production, characterization, and immobilization of a cold-adapted cutinase from Antarctic Rhodococcus sp.	1
Biochemical characterization and detection of antitumor activity of l-asparaginase from thermophilic Geobacillus kaustophilus DSM 7263T	1
The effect of the bgs13 mutation on the structure of the reporter protein beta-lactoglobulin: Influence on folding and aggregation in Pichia pastoris	1
Heterologous expression, purification and generation of specific antibodies for Annexin A8	1