Protein Expression and Purification

Article	Citations
A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies	78
Highly efficient overexpression and purification of multisubunit tethering complexes in Saccharomyces cerevisiae	22
The heat-labile toxin B subunit of E. coli fused with VP6 from GCRV (Grass carp reovirus) was expressed and folded into an active protein in rice calli	21
Screening, expression and anti-tumor functional identification of anti-LAG-3 nanobodies	19
Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells	19
Optimization of the expression of the main protease from SARS-CoV-2	17
SEC-HPLC analysis of column load and flow-through provides critical understanding of low Protein A step yield	16
Efficient expression and purification of tag-free recombinant human procalcitonin (hPCT) with precise sequence in E. coli	15
Protein inclusion into ice can dissociate subunits	15
Removal of polysorbates degradation-related impurities by depth filter screening: A case study	15
Protein expression and purification, molecular interaction, and X-ray crystallographic analysis of baculovirus protein PK2	14
Alocasia macrorrhiza rhizome lectin inhibits growth of pathogenic bacteria and human lung cancer cell in vitro and Ehrlich ascites carcinoma cell in vivo in mice	14
Editorial Board	14
Evaluation of the paired-Cas9 nickase and RNA-guided FokI genome editing tools in precise integration of an anti-CD52 bicistronic monoclonal antibody expression construct at Chinese hamster ovary cell	13
Establishment of purification method for prokaryotic expression of Serpin gene for Dermatophagoides farinae	13
Optimized vector for functional expression of the human bitter taste receptor TAS2R14 in HEK293 cells	13
Intracytoplasmic sperm injection-mediated strategy for the production of transgenic poultry as a bioreactor	13
Isolation, expression, and characterization of potato (Solanum tuberosum) GH family 17 β-1,3-glucanase (Stglu) for exploring its potential as an antifungal agent	13
Editorial Board	12
Preparation and characterization of LGR5 LOOP region-specific nanobodies	11
Simplified purification process of AsnB3 deamidated insulin variant and its comparison with human insulin	11
High-yield and cost-effective biosynthesis process for producing antimicrobial peptide AA139	11
Assessing four subdomain-specific affinity resins’ capability to separate half-antibody from intact bispecific antibody	10
Editorial Board	10
Purification of the HBV middle protein by two simple steps, application of ion exchange chromatography to improve protein homogeneity	10

Production and compositional analysis of full-length influenza virus hemagglutinin in Nanodiscs: Insights from multi-angle light scattering	10
Optimization of genetic code expansion in the baculovirus expression vector system (BEVS)	10
The N-terminal polypeptide of a new shell matrix protein hicraqin accelerates the rate of calcium carbonate deposition	10
High performance production process development and scale-up of an anti-TSLP nanobody	10
Expression and characterization of a lipase EstA from Bacillus subtilis KM-BS for application in bio-hydrolysis of waste cooking oil	9
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis	9
The long chain fatty acid-CoA ligase VraA plays a regulatory role in vancomycin resistance in Staphylococcus aureus	9
Purification and characterization of the intrinsically disordered Arabidopsis thaliana protein SOG1	9
Expression, purification, and microscopic characterization of the tumor suppressor KLHL6	9
Production of VP3-only virus-like particles of Adeno-associated virus 2 in E. coli cells	8
Editorial Board	8
One-step metal affinity purification of recombinant hFGF19 without using tags	8
Novel (R)-Hydroxynitrile lyase enzyme of Pyrus communis: Purification and characterization of its physicochemical and kinetic properties	8
Strategies for rapid production of crystallization quality coatomer WD40 domains	8
Modulation of N-glycosylation in bispecific antibody biosimilars through combined modulators	8
L-arginine interferes with functional studies of amyloid proteins	8
Novel anti-NS1 human single domain antibody from dengue infected NS1 positive patients	8
Development and expression of a factor X-derived recombinant protein for ELISA-based detection of Emicizumab	7
Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli	7
Multiple optimizations of recombinant plasmid for improving expression of Hepatitis B core antigen in Escherichia coli	7
Validation of cold shock-based technique for purification of recombinant C. elegans Phosphodiesterase 3 protein expressed in E. coli	7
Development of an effective method for purifying trypsin using a recombinant inhibitor	7
Scalable production and characterization of recombinant pneumococcal histidine triad protein D in Escherichia coli for vaccine development	7
Overproduction, purification, and transcriptional activity of recombinant Acinetobacter baylyi ADP1 RNA polymerase holoenzyme	7
Purification and characterization of recombinant human superoxide dismutase integrated with resilin-like polypeptide	7
An important consideration when expressing mAbs in Escherichia coli	7
Molecular chaperone GroEL-GroES enhances the soluble expression of biologically active ovine growth hormone in the prokaryotic system	7
Efficient heterologous expression of cellobiose 2-epimerase gene in Escherichia coli under the control of T7 lac promoter without addition of IPTG and lactose	7
Expression, characterization and anti-colon cancer activity of recombinant ginseng peptides with amino acid tandem repeats	7
Solvent extraction of recombinant interferon alpha-2b from inclusion bodies and efficient refolding at high protein concentrations	7
Bacterial expression, purification and characterization of the human Dectin-1 lectin domain for structural and functional studies	7
Molecular cloning and functional analysis of a destabilase from Hirudinaria manillensis	7
Production of recombinant human ameloblastin by a fully native purification pathway	7
Enhanced performance of Thermococcus kodakarensis KOD1 polymerase in PCR via fusion to Sulfolobus tokodaii Sto7d	6
A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing	6
Editorial Board	6
Synthesis of BLF1-containing trimethyl chitosan nanoparticles and evaluation of its immunogenicity and protection in syrian mice by oral and subcutaneous injections	6
Corrigendum to “High-affinity nanobodies targeting IL-12B for the detection of fluorescence resonance energy transfer” [Protein Express. Purificat. 229 (2025) 106681]	6
Soluble expression of hMYDGF was improved by strain engineering and optimizations of fermentation strategies in Escherichia coli	6
Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli	6
Expression and purification of Drosophila OBP44a with the aids of LC-MS and NMR	6
Biochemical characterization of a novel bifunctional alginate lyase from Microbulbifer arenaceous	6
Analysis of the in vitro function and internalization ability of a humanized EGFR antibody AE01 expressed by Chinese hamster ovary cells	6
Editorial Board	6
Deepening in the understanding of the role of collagen subunits on the differential molecular arrangement of P. glauca and M. merluccius marine collagens	6
Advantage of leaky expression, acid solubilization and CHAPS in the production of cost-effective bone morphogenetic Protein-2	6
Identification of chitinase from Bacillus velezensis strain S161 and its antifungal activity against Penicillium digitatum	6
Dual display hemagglutinin 1 and 5 on the surface of enveloped virus-like particles in silkworm expression system	6
Recombinant human FOXJ1 protein binds DNA, forms higher-order oligomers, has gel-shifting domains and contains intrinsically disordered regions	6
Zymography assisted quick purification, characterization and inhibition analysis of K. pneumoniae alkaline phosphatase by mercury and thiohydroxyal compounds	6

Unlocking the potential of Extensin Signal peptide and Elastin-like polypeptide tag fused to Shigella dysenteriae's IpaDSTxB to improve protein expression and purification in Nicotiana tabacum and Med	6
A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative	5
Navigating the Frontier: Advances in monoclonal antibody purity control	5
Preparation and characterization of a novel humanized collagen III with repeated fragments of Gly300-Asp329	5
A multidomain PARP14 construct suitable for bacterial expression	5
Strategies for manufacturing soluble human alphainterferons in Escherichia coli: expression, purification, and testing	5
Editorial Board	5
Differentially labeled flaviviral protease-cofactor complex for NMR spectroscopic applications	5
Expression of recombinant swine ferritin heavy chain with enhanced solubility in Escherichia coli and simplified purification of ferritin nanoparticles	5
Affinity gel synthesis from the p-aminobenzoic acid derivative 4-amino-2-methylbenzoic acid and purification of polyphenol oxidase from various plant sources	5
Editorial Board	5
Corrigendum to “Molecular chaperones and solubility tags: A 44-fold increase in recombinant fibEase yield for therapeutic applications” [Protein Expr. Purif. 241 (2026) 106929]	5
Efficient and economical purification platform for production of therapeutic nanobodies	5
Development of magnetic particle-based chemiluminescence immunoassay for quantitative determination of hepatitis C virus core antigen	5
Enhanced production of stem bromelain in Pichia pastoris by coexpression of unfolded protein response activator gene HAC1P	5
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm	5
High yield expression and purification of full-length Neurotensin with pyroglutamate modification	5
Soluble expression and purification of recombinant bovine ferritin H-chain	5
Construction of a novel TROP2/CD3 bispecific antibody with potent antitumor activity and reduced induction of Th1 cytokines	5
Preparation of recombinant neuritin protein	5
Improved production of class I phosphatidylinositol 4,5-bisphosphate 3-kinase	5
Purification and characterization of an asialofetuin specific lectin from the rhizome of Xanthosoma violaceum Schott	5
The construction of long-acting exendin-4 analog and its hypoglycemic effect in diabetic mice	5
Uncovering the role of leucine 59 in Renilla luciferase stability and activity with error-prone PCR: Implications for protein engineering	5
Development of a bispecific Nanobody anti-F17 fimbria as a potential therapeutic tool	5
Purification of recombinantly produced somatostatin-28 comparing hydrochloric acid and polyethyleneimine as E. coli extraction aids	5
Effective and robust separation of half-antibody byproduct in bispecific antibody purification by Sartobind Rapid A Protein A membrane chromatography	5
Expression, purification, characterization and crystallization of Panax quinquefolius ginsenoside glycosyltransferase Pq3-O-UGT2	5
Escherichia coli as a versatile cell factory: Advances and challenges in recombinant protein production	5
Optimized expression and purification of a human adenosine deaminase in E. coli and characterization of its Asp8Asn variant	5
CUREs for high-level Galectin-3 expression	5
The superiority of MabSelect VL, Cytiva's second-generation Protein L resin, over three other affinity resins in purifying a VHH-containing trispecific antibody	4
A recombinant human SLPI variant suppresses the formation of neutrophil extracellular traps at low concentrations in vitro	4
A procedure for producing an anti-AXL nanobody in E. coli	4
Fine-tuning of leaky expression delivers soluble and functional recombinant alphainterferon in the cytoplasm of Escherichia coli	4
Bioinformatics analysis and polyclonal antibody preparation of Mesocricetus auratus DHX30	4
Time- and cost-efficient bacterial expression and purification of potato apyrase	4
Characterization of a halotolerant GH2 family β-galactosidase GalM from Microvirga sp. strain MC18	4
Separation of native and C106-oxidized DJ-1 proteins by using column chromatography	4
Editorial Board	4
Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors	4
Purification and comparison of soluble and membrane-bound polyphenol oxidase from potato (Solanum tuberosum) tubers	4
Antibody-mimetic drug conjugate with efficient internalization activity using anti-HER2 VHH and duocarmycin	4
Bench-top NMR of water signals: A non-destructive tool for biomacromolecule characterization	4
Expression, purification and characterization of the transcription termination factor Rho from Azospirillum brasilense	4
Recombinant expression of antimicrobial peptide AMP-PD as tandem octamer and its antibacterial activity in vitro	4
Enhanced production of functional CRISPR-AsCas12a protein in Escherichia coli	4
A streamlined, automated workflow to screen and triage large numbers of baculoviruses for protein expression	4
Expression of a human Gb3/CD77 synthase in insect and human cells: comparison of activity and glycosylation	4
Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease	4
Expression, purification, and activity of sika deer prothymosin α protein	4
Unravelling the reason for different binding behaviors exhibited by antibody aggregates towards preparative and analytical Protein A columns	4
Development and optimization of the hypoglycemic activity of an α-glucosidase inhibitory protein from a UV-induced mutant strain of Streptomyces griseorubens	4
Functional expression of the chimera proteins of Nav1.7 and NavAb in Escherichia coli	4
Expression and functional characterization of recombinant soluble CD40 Ligand in prokaryotic systems	4
Heterologous expression, purification, and partial characterisation of the apicoplast protein 3-oxoacyl-[acyl-carrier-protein] reductase from Toxoplasma gondii	4
Sterile chicken hosts for germline transmission: A review of recipient preparation strategies in avian biotechnology	4
Mini review for niche downstream processes	4
A new combination approach to extracellular production of 5-aminolevulinic acid for purification and application in alleviating cadmium-induced oxidative stress in maize	4
Metal binding feature of copper‒induced metallothionein from freshwater crab Sinopotamon henanense reveals its Cu‒thionein character	4
Development of efficient modules for recombinant protein expression and periplasmic localisation in Pseudomonas bharatica CSV86T	4
Hyper-expression of GFP-fused active hFGF21 in tobacco chloroplasts	4
Bioinformatics analysis and preparation of polyclonal antibodies against the extracellular region of the Langya Virus attachment protein	4
A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration	4
A novel method for synthesizing authentic SARS-CoV-2 main protease	4
Identification of host cell proteins that interact with the M protein of Pigeon paramyxovirus type 1	4
A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures	4
Partition coefficient screening – An effective approach for finding the best conditions for byproduct removal as demonstrated by a bispecific antibody purification case	4
Mass production and characterization of an endoglucanase from Coleoptera insect (Monochamus saltuarius) in yeast Kluyveromyces lactis	4
Robust aggregate separation by Sartobind Rapid A Protein A membrane	4
Study on the hole and knob related impurities in the purification of bispecific antibodies	4
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica	3
Editorial Board	3
Editorial Board	3
Editorial Board	3
Cloning and characterization of a hyaluronate lyase EsHyl8 from Escherichia sp. A99	3
Heterologous expression of a novel galactose-1-phosphate uridylyltransferase from Thermodesulfatator indicus and its application for bioproduction of Gal-β-1,4-GlcNAc-X	3
Biochemical characterization of histidinol dehydrogenase from the human pathogen Neisseria gonorrhoeae	3
Optimized production and coagulation activity of rhFVII in HEK293 cells for bleeding disorders	3
Recombinant expression and purification of Plasmodium heme detoxification protein in E. coli: Challenges and discoveries	3

Column-free purification of an artificial protein nanocage, TIP60	3
A kaleidoscope of hosts: Expression systems of recombinant antibody reagents for immunological assays	3
Editorial Board	3
Molecular cloning, expression, and functional analysis of copper amine oxidase gene from mulberry (Morus alba L.)	3
Engineering an optimized expression operating unit for improved recombinant protein production in Escherichia coli	3
Engineering and characterization of GFP-targeting nanobody: Expression, purification, and post-translational modification analysis	3
Fusion with CTP increases the stability of recombinant neuritin	3
Production of enzymes for the removal of odorous substances in plant biomass	3
Evaluation of BVDV E2 proteins based on recombinant baculovirus expression system production as diagnostic antigens and immunogens	3
Design and construction of a phage-displayed Camelid nanobody library using a simple bioinformatics method	3
Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33	3
Editorial Board	3
Bioindustrial applications of thermostable Endoglucanase purified from Trichoderma viride towards the conversion of agrowastes to value-added products	3
Discovery and development of bispecific antibodies	3
Generation and characterization of a monoclonal antibody against FGFR3 that protects mice from BoNT/A	3
Enhancing the expression of multi-antigen chimeric TGAGS/BST protein from Toxoplasma gondii in Escherichia coli BL 21 Star during batch cultivation	3
Biochemical characterization and antifungal activity of a recombinant β-1,3-glucanase FlGluA from Flavobacterium sp. NAU1659	3
High soluble expression and characterization of human GalNAc transferase T2 and T11 in Escherichia coli	3
Expression and characterization of a novel β-1,4-endoglucanase from Bacillus subtilis strain isolated from a pulp and paper mill wastewater	3
Efficient development of nanobody-based affinity chromatography for AAV8 purification	3
Characterisation of ZPH13 phosphotransferase from Bacillus subtilis in zearalenone transformation	3
Fast IMAC purification of non-tagged S100A8/A9 (calprotectin) from Homo sapiens and Sus scrofa	3
Expression of recombinant preenzyme Blo t 1 allergen in Escherichia coli and its IgE reactivity	3
Prokaryotic expression of soluble IFN-λ1 recombinant protein with cold-shock system	3
Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells	3
High-throughput strategy for identification of Mycobacterium tuberculosis membrane protein expression conditions using folding reporter GFP	3
Bacterially expressed full length Hemagglutinin of Avian Influenza Virus H5N1 forms oligomers and exhibits hemagglutination	3
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites	3
Enzymatic characterization and thermostability improvement of an acidophilic endoxylanase PphXyn11 from Paenibacillus physcomitrellae XB	3
Efficient production of recombinant human FVII in CHO cells using the piggyBac transposon system	3
Biochemical and structural characterization of a novel L-isoleucine-4-dioxygenase (RaIDO) from Rahnella aquatilis	3
Optimization of the heterologous expression and purification of Plasmodium falciparum generative cell specific 1 in Escherichia coli	3
The combined effect of the gene copy number and chaperone overexpression on the recombinant bovine chymosin production in Pichia pastoris, with mutant ADH2 promoter	3
Production of recombinant human proliferating cellular nuclear antigen (PCNA) for structural and biophysical characterization	3
StxB fusion strategy for immunogenic enhancement: Recombinant expression of AcAMP antimicrobial peptide	3
Editorial Board	3
Expression, purification and preliminary crystallographic analysis of bacterial transmembrane protein EfeU for iron import	3
Editorial Board	3
Soluble expression of recombinant human interleukin-2 in Escherichia coli and its facile production	3
Deciphering the inconsistent performance of Protein A membrane on host cell protein clearance	3
Editorial Board	3
Optimized isolation of enzymatically active ubiquitin E3 ligase E6AP/UBE3A from mammalian cells	3
E. coli chromosomal-driven expression of NADK2 from A. thaliana: A preferable alternative to plasmid-driven expression for challenging proteins	3
Use of the redox-dependent intein system for enhancing production of the cyclic green fluorescent protein	3
Kinetic modeling: A tool for temperature shift and feeding optimization in cell culture process development	3
Recombinant highly antigenic truncated fusion-based protein as a diagnostic antigen for anti-SARS-CoV-2 nucleocapsid antibody ELISA	2
Advancing recombinant antibody production in E. coli: Optimization of expression and purification via dual GFP promoter and imaging technology	2
Enhanced expression and solubility of main protease (Mpro) of SARS-CoV-2 from E. coli	2
Thermostable phenylacetic acid degradation protein TtPaaI from Thermus thermophilus as a scaffold for tetravalent display of proteins	2
Tetrahymena thermophila granule lattice protein 3 improves solubility of sexual stage malaria antigens expressed in Escherichia coli	2
A two-step method preparation of semaglutide through solid-phase synthesis and inclusion body expression	2
Optimization of expression and renaturation methods for the production of a recombinant fibrinolytic protease showing in vivo antithrombotic activity	2
Solubilization and refolding of inclusion bodies by detergents	2
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Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM	2
Editorial Board	2
Extraction and characterization of functional duckweed proteins using alkaline, enzymatic, and ultrasound-assisted techniques	2
Improved self-cleaving precipitation tags for efficient column free bioseparations	2
Hyper-expression of functional human basic fibroblast growth factor 2 in Escherichia coli	2
Viral removal by column chromatography in downstream processing of monoclonal antibodies	2
Protein production in transgenic chickens mediated through primordial germ cells and embryonic stem cells	2
Strategic truncation improves recombinant expression of buffalo Fibulin-5 (FBLN5) in Escherichia coli	2
CHO cathepsin B identified as the protease responsible for a target bispecific antibody fragmentation	2
Efficient production of human mitochondrial chaperonin (Hsp60/Hsp10) in Escherichia coli using a polyprotein strategy	2
Screening and functional characterization of nanobodies targeting the transferrin receptor	2
Functional characterization and structural insights of three cutinases from the ascomycete Fusarium verticillioides	2
Resin and loading condition screening for effective and robust byproducts removal by anion exchange chromatography: A case study	2
Light-induced expression of a novel marine laccase in Escherichia coli from Marinomonas profundimaris and its application in synthetic dye decolorization	2
Discovery and biochemical characterization of two hexokinases from Crassostrea gigas	2
Electroporation of SUMO-His-Cre protein triggers a specific recombinase-mediated cassette exchange in HEK 293T cells	2
Purification and characterization of full-length monomeric TEC family kinase, ITK	2
Purification and characterization of eukaryotic ATP-dependent transporters homologously expressed in Pichia pastoris for structural studies by cryo-electron microscopy	2
Scalable approach for coronavirus-like particles making based on the spike protein using Sf9 cells	2
Overexpression and truncation of a novel cold-adapted lipase with improved enzymatic characteristics	2
Adaptation of induced pluripotent stem cell technology for avian species	2
Synergistic effect of LTB and Mn2+ adjuvants on immunogenicity of multi-epitope peptides from Staphylococcus aureus	2
Heterologous expression and biochemical comparison of two homologous SoxX proteins of endosymbiotic Candidatus Vesicomyosocius okutanii and free-living Hydrogenovibrio crunogenus from deep-sea environ	2
Editorial Board	2
Expression, purification and functional study of mycobacteriophage D29 histidine-asparagine-histidine endonuclease	2
Characterization of a novel GH5 β-mannanase from Hahella sp. CR1 and its synergistic role in degradation of spent coffee grounds	2
Cell-free protein production of a gamma secretase homolog	2
Overcoming fluorescence loss in mEOS-based AAA+ unfoldase reporters through covalent linkage	2
Heterologous expression, purification and single step efficient refolding of recombinant tissue plasminogen activator (Reteplase) from E. coli	2
Comparative analysis of SUMO and his-tag fusion for soluble expression of the SARS-CoV-2 omicron RBD in E. coli	2
Purification of antifreeze proteins from tussah silkworm (Antheraea pernyi) via polyethylene glycol precipitation coupled with aqueous two-phase extraction	2
Production and functional analysis of a phage displayed scFv recombinant antibody targeting EGFR/HER2 dimerization domain	2
Investigation and development of transient production process for porcine circovirus Type-2 (PCV2) capsid protein in HEK293F cells	2
Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana: Characterization of the enzyme's in vitro truncation by thiol endopeptidase activity	2
A self-cleavable ULP1-SUMO fusion strategy for enhanced soluble protein expression and one-step purification in Escherichia coli	2
Characterization of a bifunctional and endolytic alginate lyase from Microbulbifer sp. ALW1 and its application in alginate oligosaccharides production from Laminaria japonica	2
Additive-based protein stabilization for stress-prone and unstable proteins	2
Comparative biophysical and functional analysis of TCZ-UFRJ, a potential biosimilar to Actemra	2
Editorial Board	2
Avian primordial germ cell migration: History, mechanisms, applications, and unanswered questions	2