Current Opinion in Biotechnology

Article	Citations
Deep learning for optimization of protein expression	243
Virome studies of food production systems: time for ‘farm to fork’ analyses	191
Strategies for uncovering stable isotope tracing patterns between cell populations	154
Stop helping pathogens: engineering plant susceptibility genes for durable resistance	103
Recent progress in the synthesis of advanced biofuel and bioproducts	100
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	99
Assessing the potential to harness the microbiome through plant genetics	95
Biofabrication strategies for cardiac tissue engineering	90
Recent advances in bivalve-microbiota interactions for disease prevention in aquaculture	90
The limitless applications of single-cell metabolomics	90
Avoidance of detrimental defense responses in beneficial plant–microbe interactions	83
Editorial overview: Biotechnology to help understand and harness biotic interactions in plants	82
Novel transducers for high-channel-count neuroelectronic recording interfaces	80
Bioelectrosynthesis systems	79
Editorial Board	76
Editorial Board	73
Editorial overview: Tissue, cell, and pathway engineering: Recent innovations in biomedical technologies	72
Autophagy as a critical driver of metabolic adaptation, therapeutic resistance, and immune evasion of cancer	71
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	69
Pathway elucidation and engineering of plant-derived diterpenoids	68
Constructing next-generation CRISPR–Cas tools from structural blueprints	66
Recent advances of machine learning applications in human gut microbiota study: from observational analysis toward causal inference and clinical intervention	66
Editorial overview: Analytical biotechnology for healthcare, strain engineering, biosensing and synthetic biology	65
Biohybrid approaches to interface with the nervous system: the best of both worlds	65
Systems biology-based analysis of cell-free systems	65

Editorial Board	65
Recent advances in microbial community analysis from machine learning of multiparametric flow cytometry data	64
The dawn of interoperating spatial models in cell biology	63
High-Throughput Functional Genomics for Energy Production	60
Editorial Board	60
Engineering healthy crops: molecular strategies for enhancing the plant immune system	60
Inhalable mRNA vaccines for respiratory diseases: a roadmap	60
Role of biotechnology in the advancement of biodegradable polymers and functionalized additives for food packaging systems	58
Progress toward rapid, at-line N-glycosylation detection and control for recombinant protein expression	56
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	56
Stem cells characterization: OMICS reinforcing analytics	56
Engineering protein nanoparticles for drug delivery	54
Biotechnological interventions in reducing losses of tropical fruits and vegetables	54
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	53
Isotope tracing in health and disease	52
Adaptive biomimicry: design of neural interfaces with enhanced biointegration	51
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	49
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	48
Jump-starting chimeric antigen receptor-T cells to go the extra mile with nanotechnology	47
De novo domestication: retrace the history of agriculture to design future crops	46
The yin and yang of itaconate metabolism and its impact on the tumor microenvironment	46
Bioengineering applications for hearing restoration: emerging biologically inspired and biointegrated designs	46
NLRP3 as a sensor of metabolism gone awry	45
Transdermal sensing: in-situ non-invasive techniques for monitoring of human biochemical status	45
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	44
Bio-based antimicrobial compositions and sensing technologies to improve food safety	43
Mediated electrochemistry for redox-based biological targeting: entangling sensing and actuation for maximizing information transfer	42
Barriers to microalgal mass cultivation	42
Microbial single-cell mass spectrometry: status, challenges, and prospects	42
Practical monitoring technologies for cells and substrates in biomanufacturing	42
Aspergillus terreus as an industrial filamentous fungus for pharmaceutical biotechnology	41
Coral holobionts and biotechnology: from Blue Economy to coral reef conservation	41
Biotechnology for secure biocontainment designs in an emerging bioeconomy	41
Cell-free technologies for biopharmaceutical research and production	41
Recent advances in heterologous expression of natural product biosynthetic gene clusters in Streptomyces hosts	41
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	40
Synthetic molecular evolution of antimicrobial peptides	40
Application of bacteriophage-derived endolysins to combat streptococcal disease: current state and perspectives	40
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	39
Recent advances of surface acoustic wave-based sensors for noninvasive cell analysis	39
Yeast based biorefineries for oleochemical production	39
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	39
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	37
Chitosan and other edible coatings to extend shelf life, manage postharvest decay, and reduce loss and waste of fresh fruits and vegetables	37
Microbial inefficient substrate use through the perspective of resource allocation models	37
Metabolic engineering and fermentation of microorganisms for carotenoids production	36
Using plant chemistry to improve interactions between plants, herbivores and their natural enemies: challenges and opportunities	36
Advances in automated and reactive flow cytometry for synthetic biotechnology	36
Coupling and uncoupling growth and product formation for producing chemicals	35
Stem cell engineering approaches for investigating glial cues in central nervous system disorders	35

Engineering bacterial warriors: harnessing microbes to modulate animal physiology	35
Can digital twin efforts shape microorganism-based alternative food?	34
Machine learning for predicting phenotype from genotype and environment	34
Engineering highly productive cyanobacteria towards carbon negative emissions technologies	34
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	34
Mining natural products for advanced biofuels and sustainable bioproducts	33
An ossifying landscape: materials and growth factor strategies for osteogenic signalling and bone regeneration	33
Roots of synthetic ecology: microbes that foster plant resilience in the changing climate	33
Recent advances in synthetic glycoengineering for biological applications	33
Recent progress and advanced technology in carbohydrate-based drug development	32
Plant virus-interactions: unraveling novel defense mechanisms under immune-suppressing pressure	32
Harnessing the omics revolution to address the global biodiversity crisis	32
Editorial overview: The future of food biotechnology is microbial	32
Continuous Plankton Recorder in the omics era: from marine microbiome to global ocean observations	31
Synthetic or natural? Metabolic engineering for assimilation and valorization of methanol	31
Advances in quantifying circulatory microRNA for early disease detection	30
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	30
Chain elongators, friends, and foes	29
Biotic–abiotic hybrids for bioanalytics and biocatalysis	29
Industrializing methanotrophs and other methylotrophic bacteria: from bioengineering to product recovery	29
Creating an atlas to visualize the biodiversity of the mammalian gut microbiota	29
Mineralized collagen scaffolds for regenerative engineering applications	29
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	28
Strategies for engineering improved nitrogen use efficiency in crop plants via redistribution and recycling of organic nitrogen	28
Large-scale production of enzymes for biotechnology uses	28
Enhancing phage therapy through synthetic biology and genome engineering	28
Native LC–MS for capturing quality attributes of biopharmaceuticals on the intact protein level	28
Biosynthesis of human milk oligosaccharides via metabolic engineering approaches: current advances and challenges	28
Creating value from purple phototrophic bacteria via single-cell protein production	28
Toward sustainable, cell-free biomanufacturing	28
Experimental infrastructure requirements for quantitative research on microbial communities	28
Toward optimal use of biomass as carbon source for chemical bioproduction	28
Circadian rhythms and meal timing: impact on energy balance and body weight	28
Phage-based target discovery and its exploitation towards novel antibacterial molecules	28
The prospect of superior yeast for winemaking: recent successes through bioprospecting	28
Illuminating the cross-talk between tumor metabolism and immunity in IDH-mutated cancers	28
Methane to bioproducts: unraveling the potential of methanotrophs for biomanufacturing	27
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	27
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	27
The phosphorus challenge: biotechnology approaches for a sustainable phosphorus system	27
Advances in consolidated bioprocessing using synthetic cellulosomes	27
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	27
Tumor interstitial fluid analysis enables the study of microenvironment–cell interactions in cancers	27
Transient electronics: new opportunities for implantable neurotechnology	26
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	26
Engineering plant–microbe communication for plant nutrient use efficiency	26
Biosensor-enabled pathway optimization in metabolic engineering	26
Bacterial degradation of perfluoroalkyl acids	26
Upstream continuous processing: recent advances in production of biopharmaceuticals and challenges in manufacturing	26
Manufactured extracellular vesicles as human therapeutics: challenges, advances, and opportunities	26
Enhancing phytoremediation of soils polluted with heavy metals	26
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	25
Protein engineering for electrochemical biosensors	25
Corrigendum to “Unraveling the mechanobiology of immune cells” [Curr Opin Biotechnol 66 (2020) 236-245]	25
Synthetic co-cultures: novel avenues for bio-based processes	25
Engineered diets to improve cancer outcomes	25
Design and engineering of artificial microbial consortia for biohydrogen production	25
Two in one: the emerging concept of bifunctional antibodies	24
Single-cell analysis opens a goldmine for plant functional studies	24
Editorial Board	24
Discovery of new enzymatic functions and metabolic pathways using genomic enzymology web tools	24
Designer protein compartments for microbial metabolic engineering	24
Biotechnological approaches for predicting and controlling apple storage disorders	24
General framework for enzyme-photo-coupled catalytic system toward carbon dioxide conversion	24
Editorial overview: Energy biotechnology as an integral solution to global challenges	23
Engineering of citrus to obtain huanglongbing resistance	23
Bridging the gap — biomimetic design of bioelectronic interfaces	23
Interactions between nanoparticle corona proteins and the immune system	23
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	23
Life stage-dependent genetic traits as drivers of plant–herbivore interactions	23
Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks	23
Putting the pieces together: mapping the O-glycoproteome	23
Editorial overview: Pharmaceutical biotechnology	23
Editorial Board	23
Mycotoxin risks are lower in biotech corn	23
Solar salterns as model systems to study the units of bacterial diversity that matter for ecosystem functioning	23
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	22
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	22
Pulses-derived proteins for the plant-based market: opportunities to reduce postharvest loss and waste	22
Applications of targeted proteomics in metabolic engineering: advances and opportunities	22

Editorial overview: Pharmaceutical biotechnology: the pandemic years — new modality development and biomanufacturing innovations in a maturing field	22
Editorial overview: Tissue, cell, and pathway engineering	22
Editorial Board	21
Editorial Board	21
Editorial Board	21
Innovations and trends in antibody repertoire analysis	21
Beyond calorie restriction: aging as a biological target for nutrient therapies	21
Advances in generative modeling methods and datasets to design novel enzymes for renewable chemicals and fuels	21
Editorial overview: Chemical Biotechnology	21
Deep learning in regulatory genomics: from identification to design	21
Food value chain interventions and nutritional outcomes: a review of evidence and recommendations for future assessments	21
Editorial Board	21
Discovery and application of dietary compounds to optimize human health, a focus on skeletal muscle regeneration	21
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	21
Coupling chemistry and biology for the synthesis of advanced bioproducts	20
Senescent cells in tissue engineering	20
Nanoencapsulation application to prolong postharvest shelf life	20
Status and challenges of electrical stimulation use in chronic wound healing	20
In vivo gene delivery to immune cells	20
The plant microbiota: composition, functions, and engineering	20
Future directions in microbial nitrogen cycling in wastewater treatment	20
Antifungal natural products	20
Glutathione and its precursors in cancer	20
Advances in genome-scale metabolic models of industrially important fungi	19
Recent advances in prime editing technologies and their promises for therapeutic applications	19
Enhancing enzymatic performance with nanoparticle immobilization: improved analytical and control capability for synthetic biochemistry	19
Editorial Board	19
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	19
Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products	19
A molecular assessment of the practical potential of DNA-based computation	19
In situ characterization of the tumor microenvironment	19
Engineering the supernatural: monoclonal antibodies for challenging infectious diseases	19