Current Opinion in Biotechnology

Article	Citations
Editorial Board	277
Editorial Board	219
Systems biology-based analysis of cell-free systems	175
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	166
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	115
Recent progress in the synthesis of advanced biofuel and bioproducts	104
Stem cells characterization: OMICS reinforcing analytics	100
Bioelectrosynthesis systems	96
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	95
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	94
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	91
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	90
Cell-free technologies for biopharmaceutical research and production	86
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	83
Creating value from purple phototrophic bacteria via single-cell protein production	83
Circadian rhythms and meal timing: impact on energy balance and body weight	82
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	79
Editorial Board	77
Leveraging rAAV bioprocess understanding and next generation bioanalytics development	75
Innovations and trends in antibody repertoire analysis	75
Engineered living bioassemblies for biomedical and functional material applications	75
Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products	73
Single-cell analysis opens a goldmine for plant functional studies	72
Engineering the plant microbiota in the context of the theory of ecological communities	71
Deep learning in regulatory genomics: from identification to design	69

The plant microbiota: composition, functions, and engineering	68
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	66
Lighting the way: recent developments and applications in molecular optogenetics	61
Bridging the gap — biomimetic design of bioelectronic interfaces	61
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	59
Emerging technologies for genetic control systems in cellular therapies	59
Coupling chemistry and biology for the synthesis of advanced bioproducts	59
Fermented food products in the era of globalization: tradition meets biotechnology innovations	58
A molecular assessment of the practical potential of DNA-based computation	58
Multifaceted metabolic role of infections in the tumor microenvironment	55
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	55
Computational tools and resources for designing new pathways to small molecules	55
Engineering of the genetic code	53
Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control	51
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	51
Dietary microalgae on poultry meat and eggs: explained versus unexplained effects	51
Mass spectrometry imaging methods for visualizing tumor heterogeneity	51
2-hydroxyglutarate rides the cancer-immunity cycle	50
Contemporary aquaculture: implications for human nutrition	49
Machine learning to navigate fitness landscapes for protein engineering	48
Perspectives for self-driving labs in synthetic biology	48
Using genome and transcriptome analysis to elucidate biosynthetic pathways	46
Editorial Board	45
Editorial overview: Biotechnological insights of marine and terrestrial microbiome	45
Editorial Board	44
Plant synthetic biology as a tool to help eliminate hidden hunger	44
Computational approaches to understanding nutrient metabolism and metabolic disorders	44
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	43
Harnessing in vitro platforms for natural product research: in vitro driven rational engineering and mining (iDREAM)	43
Toolboxes for plant systems biology research	43
Advancing in vivo reprogramming with synthetic biology	42
Integration of omics data to unravel root microbiome recruitment	42
Reducing crop losses by gene-editing control of organ developmental physiology	42
Analytics of host cell proteins (HCPs): lessons from biopharmaceutical mAb analysis for Gene therapy products	41
Anaerobic fluorescent reporters for cell identification, microbial cell biology and high-throughput screening of microbiota and genomic libraries	39
Sourcing DNA parts for synthetic biology applications in plants	39
Matching diverse feedstocks to conversion processes for the future bioeconomy	39
Recent advances in biotechnology for marine enzymes and molecules	39
The potential of amoeba-based processes for natural product syntheses	39
Impact of anthropogenic contamination on glacier surface biota	38
Redox as a bioprocess parameter: analytical redox quantification in biological therapeutic production	38
Editorial overview: Emerging routes to sustainable energy capture and conversion into value-added products	38
Synthetic biology approaches for dynamic CHO cell engineering	38
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	38
Understanding atmospheric intercontinental dispersal of harmful microorganisms	37
Metabolic and cellular engineering for the production of natural products	37
Synthetic apomixis: the beginning of a new era	37
Single-cell omics analysis with genome-scale metabolic modeling	37
Low-cost, point-of-care biomarker quantification	37
Overview of dietary assessment methods for measuring intakes of foods, beverages, and dietary supplements in research studies	37

Matchmaking at the cell surface using bispecifics to put cells on their best behavior	36
Programmable synthetic biology tools for developing microbial cell factories	36
Ketogenic regimens for acute neurotraumatic events	36
Complexity of subcellular metabolism: strategies for compartment-specific profiling	35
Editorial Board	35
The aging tumor metabolic microenvironment	34
Editorial overview: Nanobiotechnology for immunoengineering	34
Synthetic fuels: what are they and where do they come from?	34
Engineered methane biocatalysis: strategies to assimilate methane for chemical production	33
Synthetic mRNA – emerging new class of drug for tissue regeneration	33
Extracellular vesicles as therapeutics for inflammation and infection	33
What’s new in single-cell proteomics	33
Engineering insect resistance using plant specialized metabolites	32
Editorial Board	32
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	32
Potential use of microbial engineering in single-cell protein production	32
Avoidance of detrimental defense responses in beneficial plant–microbe interactions	32
Targeting ripening regulators to develop fruit with high quality and extended shelf life	32
Next-generation biological vector platforms for in vivo delivery of genome editing agents	32
Editorial overview: Biotechnology to help understand and harness biotic interactions in plants	32
Antigen-specific nanomedicines for the treatment of autoimmune disease: target cell types, mechanisms and outcomes	32
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	31
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	31
Strategies for uncovering stable isotope tracing patterns between cell populations	31
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	31
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	30
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	30
Inhalable mRNA vaccines for respiratory diseases: a roadmap	30
Practical monitoring technologies for cells and substrates in biomanufacturing	30
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	30
Biohybrid approaches to interface with the nervous system: the best of both worlds	30
Machine learning for predicting phenotype from genotype and environment	30
Can digital twin efforts shape microorganism-based alternative food?	29
Large-scale production of enzymes for biotechnology uses	29
De novo domestication: retrace the history of agriculture to design future crops	29
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	29
Advances in engineering substrate scope of Pseudomonas cell factories	29
Editorial overview: Tissue, cell, and pathway engineering	29
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	29
Editorial Board	29
Engineering plant–microbe communication for plant nutrient use efficiency	28
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	28
Two in one: the emerging concept of bifunctional antibodies	28
Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks	28
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	28
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	28
In situ characterization of the tumor microenvironment	28
Sustainability in food-waste reduction biotechnology: a critical review	28
Senescent cells in tissue engineering	28
Life stage-dependent genetic traits as drivers of plant–herbivore interactions	28
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	27
Corrigendum to “Unraveling the mechanobiology of immune cells” [Curr Opin Biotechnol 66 (2020) 236-245]	27
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	27
General framework for enzyme-photo-coupled catalytic system toward carbon dioxide conversion	27
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	27
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	26
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	26
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	26
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	25
Advances in engineering algae for biofuel production	25
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	25
Division of labor for substrate utilization in natural and synthetic microbial communities	25
Computational models in systems biology: standards, dissemination, and best practices	25
Progress and challenges in the translation of cancer nanomedicines	25
Mycotoxin risks are lower in biotech corn	25
Force in numbers: high-throughput screening approaches to unlock microbial transport	25
Engineering bacteria for cancer immunotherapy	24
Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics	24
Building mutational bridges between carbohydrate-active enzymes	24
In vivo 2H/13C flux analysis in metabolism research	24
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	24
Chemical speciation of trace metals in mammalian cell culture media: looking under the hood to boost cellular performance and product quality	24
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	24
Approaches for a more microbiologically and chemically safe dried fruit supply chain	24
Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	24
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	24
Quantifying nanoparticle delivery: challenges, tools, and advances	24
Carbon recycling with synthetic CO2 fixation pathways	24
RNAs — a new frontier in crop protection	23
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	23

Drugging the microbiome and bacterial live biotherapeutic consortium production	23
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	23
Cell-free enzyme cascades — application and transition from development to industrial implementation	23
Enzymes in biotechnology: Critical platform technologies for bioprocess development	23
Cellobiose dehydrogenase in biofuel cells	23
Engineering microbial consortia with rationally designed cellular interactions	23
Technologies for the discovery of G protein–coupled receptor–targeting biologics	22
Transcription factor-based biosensors: a molecular-guided approach for natural product engineering	22
Engineering organoids as cerebral disease models	22
Cytochromes P450 in the biocatalytic valorization of lignin	22
Continuous biomanufacturing with microbes — upstream progresses and challenges	22
Editorial Board	22
Biomolecular feedback controllers: from theory to applications	22
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	22
Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces	21
Editorial overview: Tissue, cell and pathway engineering: bioinspired, biomimetic and hybrid neural interfaces	21
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	21
Microbial production of chemicals driven by CRISPR-Cas systems	21
Utilizing cross-species co-cultures for discovery of novel natural products	21
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	21
Time to integrate biotechnological approaches into fish gut microbiome research	21
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	21
Optogenetics in medicine: innovations and therapeutic applications	21
SgRNA engineering for improved genome editing and expanded functional assays	20
Technoeconomic analysis for near-term scale-up of bioprocesses	20
Sharp solutions to cleave plant fibers	20
The double life of CRISPR–Cas13	20
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	20
Engineered microbial living matter for diagnostics, prevention, and therapy	20
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	20
Filamentous fungi for future functional food and feed	20
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	20
Artificial intelligence: a solution to involution of design–build–test–learn cycle	19
Surveilling cellular vital signs: toward label-free biosensors and real-time viability assays for bioprocessing	19
Petrosia ficiformis (Poiret, 1789): an excellent model for holobiont and biotechnological studies	19
Editorial overview: 21st Century solutions for crop improvement	19
Bio-integrative design of the neural tissue-device interface	19
Cell–nano interactions of polydopamine nanoparticles	19
Identification of novel membrane proteins for improved lignocellulose conversion	19
Cutting-edge plant natural product pathway elucidation	19
Ultrasonographic features of ovarian morphology capture nutritional and metabolic influences on the reproductive axis: implications for biomarker development in ovulatory disorders	19
Rational design of peptide-based implants for corneal bioengineering	19
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	18
Recent advances in synthetic glycoengineering for biological applications	18
Cell-free chemoenzymatic cascades with bio-based molecules	18
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	18
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	18
Dissecting postharvest chilling injury through biotechnology	18
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	18
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	18
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	18
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	18
Spatial omics techniques and data analysis for cancer immunotherapy applications	18
Cell-free protein synthesis systems for vaccine design and production	18
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	17
Transdermal sensing: in-situ non-invasive techniques for monitoring of human biochemical status	17
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	17
Coral holobionts and biotechnology: from Blue Economy to coral reef conservation	17
Adaptive biomimicry: design of neural interfaces with enhanced biointegration	17
Constructing next-generation CRISPR–Cas tools from structural blueprints	17
Coupling and uncoupling growth and product formation for producing chemicals	17
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	17
Harnessing the omics revolution to address the global biodiversity crisis	17
Editorial Board	17
The dawn of interoperating spatial models in cell biology	17
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	17
Biotechnological interventions in reducing losses of tropical fruits and vegetables	17
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	17
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	16
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	16
Advances in mass spectrometry-enabled multiomics at single-cell resolution	16
Transient electronics: new opportunities for implantable neurotechnology	16
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	16
AuxSynBio: synthetic biology tools to understand and engineer auxin	16
Different avenues for progress apply to drought tolerance, water use efficiency and yield in dry areas	16
Biosensor-enabled pathway optimization in metabolic engineering	16
Editorial overview: Pharmaceutical biotechnology	16
The tier system: a host development framework for bioengineering	16
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	16
Antifungal natural products	16
Advances in genome-scale metabolic models of industrially important fungi	16
Engineering of citrus to obtain huanglongbing resistance	16
Beyond co-expression: pathway discovery for plant pharmaceuticals	15
Design and fabrication of field-deployable microbial biosensing devices	15
Using systems metabolic engineering strategies for high-oil maize breeding	15
Advances in vascularization and innervation of constructs for neural tissue engineering	15
Does the surface charge of the nanoparticles drive nanoparticle–cell membrane interactions?	15
Editorial Board	15
Diversity: current and prospective secondary metabolites for nutrition and medicine	15
Machine learning for synthetic gene circuit engineering	15
Carbonic anhydrase for CO2 capture, conversion and utilization	15
Personalized gut microbial community modeling by leveraging genome-scale metabolic models and metagenomics	15
Mannosylerythritol lipids: production, downstream processing, and potential applications	15
Principles for designing an optimal mRNA lipid nanoparticle vaccine	15
Molecular characterization of HEK293 cells as emerging versatile cell factories	15