Current Opinion in Biotechnology

Article	Citations
Systems biology-based analysis of cell-free systems	268
Editorial Board	217
Editorial Board	132
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	125
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	119
Bioelectrosynthesis systems	117
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	116
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	107
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	102
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	98
Anaerobic microbial methanol utilization as a one-carbon feedstock	97
Cell-free technologies for biopharmaceutical research and production	96
Recent progress in the synthesis of advanced biofuel and bioproducts	95
Creating value from purple phototrophic bacteria via single-cell protein production	92
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	90
Engineered T cells and macrophages: two arms to seize solid tumors	88
Innovations and trends in antibody repertoire analysis	74
Editorial Board	72
Leveraging rAAV bioprocess understanding and next generation bioanalytics development	71
Single-cell analysis opens a goldmine for plant functional studies	68
Coupling chemistry and biology for the synthesis of advanced bioproducts	67
Editorial Board	63
Bridging the gap — biomimetic design of bioelectronic interfaces	62
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	60
A molecular assessment of the practical potential of DNA-based computation	56

Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	55
Emerging technologies for genetic control systems in cellular therapies	55
Deep learning in regulatory genomics: from identification to design	55
The plant microbiota: composition, functions, and engineering	54
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	54
Lighting the way: recent developments and applications in molecular optogenetics	54
Engineered living bioassemblies for biomedical and functional material applications	53
2-hydroxyglutarate rides the cancer-immunity cycle	53
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	52
Engineering of the genetic code	51
Using genome and transcriptome analysis to elucidate biosynthetic pathways	50
Multifaceted metabolic role of infections in the tumor microenvironment	50
Computational tools and resources for designing new pathways to small molecules	48
Machine learning to navigate fitness landscapes for protein engineering	48
Mass spectrometry imaging methods for visualizing tumor heterogeneity	47
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	46
Perspectives for self-driving labs in synthetic biology	46
Dietary microalgae on poultry meat and eggs: explained versus unexplained effects	46
Harnessing the potential of microbial methane utilization for chasing sustainability	45
Editorial overview: Biotechnological insights of marine and terrestrial microbiome	45
Reducing crop losses by gene-editing control of organ developmental physiology	45
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	45
Toolboxes for plant systems biology research	44
Editorial Board	44
Matching diverse feedstocks to conversion processes for the future bioeconomy	44
Advancing in vivo reprogramming with synthetic biology	43
Low-cost, point-of-care biomarker quantification	42
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	42
Metabolic and cellular engineering for the production of natural products	42
Biochemical production with microbial bioelectrochemical systems	42
Plant synthetic biology as a tool to help eliminate hidden hunger	42
Editorial overview: Pioneering innovations in food biotechnology	41
Understanding atmospheric intercontinental dispersal of harmful microorganisms	41
Sourcing DNA parts for synthetic biology applications in plants	41
Pathway engineering for the biosynthesis of psychedelics	41
Editorial overview: Emerging routes to sustainable energy capture and conversion into value-added products	40
The potential of amoeba-based processes for natural product syntheses	40
Synthetic biology approaches for dynamic CHO cell engineering	40
Synthetic apomixis: the beginning of a new era	40
Impact of anthropogenic contamination on glacier surface biota	40
Single-cell omics analysis with genome-scale metabolic modeling	40
Synthetic fuels: what are they and where do they come from?	39
Editorial overview: Nanobiotechnology for immunoengineering	39
Editorial Board	39
Targeting ripening regulators to develop fruit with high quality and extended shelf life	39
Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes	39
Understanding and harnessing the complexity of interspecies interactions in acetogenic mixotrophic co-cultures	38
Antigen-specific nanomedicines for the treatment of autoimmune disease: target cell types, mechanisms and outcomes	38
Editorial Board	38
Matchmaking at the cell surface using bispecifics to put cells on their best behavior	37

Engineered methane biocatalysis: strategies to assimilate methane for chemical production	37
Complexity of subcellular metabolism: strategies for compartment-specific profiling	37
What’s new in single-cell proteomics	37
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	37
Synthetic mRNA – emerging new class of drug for tissue regeneration	37
The aging tumor metabolic microenvironment	37
Programmable synthetic biology tools for developing microbial cell factories	36
Extracellular vesicles as therapeutics for inflammation and infection	36
Next-generation biological vector platforms for in vivo delivery of genome editing agents	36
Potential use of microbial engineering in single-cell protein production	35
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	35
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	35
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	35
Editorial Board	35
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	34
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	34
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	33
Biohybrid approaches to interface with the nervous system: the best of both worlds	33
Can digital twin efforts shape microorganism-based alternative food?	33
Machine learning for predicting phenotype from genotype and environment	33
Inhalable mRNA vaccines for respiratory diseases: a roadmap	33
In situ characterization of the tumor microenvironment	32
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	32
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	32
Strategies for uncovering stable isotope tracing patterns between cell populations	32
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	32
De novo domestication: retrace the history of agriculture to design future crops	32
Editorial Board	32
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	31
Senescent cells in tissue engineering	30
Editorial overview: Tissue, cell, and pathway engineering	30
Editorial Board	30
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	30
Corrigendum to “Unraveling the mechanobiology of immune cells” [Curr Opin Biotechnol 66 (2020) 236-245]	29
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	29
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	29
Progress and challenges in the translation of cancer nanomedicines	29
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	29
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	29
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	29
Mycotoxin risks are lower in biotech corn	28
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	28
Division of labor for substrate utilization in natural and synthetic microbial communities	28
General framework for enzyme-photo-coupled catalytic system toward carbon dioxide conversion	28
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	28
Two in one: the emerging concept of bifunctional antibodies	28
Advances in engineering substrate scope of Pseudomonas cell factories	28
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	28
Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks	28
Recent advances in microbial production of rare sugars and steviols	27
Sustainability in food-waste reduction biotechnology: a critical review	27
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	27
Engineering plant–microbe communication for plant nutrient use efficiency	27
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	26
Advances in engineering algae for biofuel production	26
Carbon recycling with synthetic CO2 fixation pathways	26
Computational models in systems biology: standards, dissemination, and best practices	26
Force in numbers: high-throughput screening approaches to unlock microbial transport	25
Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	25
Quantifying nanoparticle delivery: challenges, tools, and advances	25
Biomolecular feedback controllers: from theory to applications	25
Engineering bacteria for cancer immunotherapy	25
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	25
Engineering microbial consortia with rationally designed cellular interactions	25
Recent advances in microbial synthesis of polyphenols	25
Anaerobic microbial core for municipal wastewater treatment — the sustainable platform for resource recovery	25
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	25
Building mutational bridges between carbohydrate-active enzymes	24
Drugging the microbiome and bacterial live biotherapeutic consortium production	24
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	24
Cellobiose dehydrogenase in biofuel cells	24
Approaches for a more microbiologically and chemically safe dried fruit supply chain	24
Cytochromes P450 in the biocatalytic valorization of lignin	24
Technologies for the discovery of G protein–coupled receptor–targeting biologics	23
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	23
Filamentous fungi for future functional food and feed	23
Editorial overview: Phosphate Special Issue	23
Cell-free enzyme cascades — application and transition from development to industrial implementation	23
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	23
Continuous biomanufacturing with microbes — upstream progresses and challenges	23

Engineering organoids as cerebral disease models	23
Editorial overview: Tissue, cell and pathway engineering: bioinspired, biomimetic and hybrid neural interfaces	22
Technoeconomic analysis for near-term scale-up of bioprocesses	22
Machine learning in wastewater: opportunities and challenges — “not everything is a nail!”	22
The double life of CRISPR–Cas13	22
Time to integrate biotechnological approaches into fish gut microbiome research	22
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	22
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	22
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	22
Microbial production of chemicals driven by CRISPR-Cas systems	22
Engineered microbial living matter for diagnostics, prevention, and therapy	22
SgRNA engineering for improved genome editing and expanded functional assays	22
Optogenetics in medicine: innovations and therapeutic applications	22
Window of sustainable bioprocess operation: towards merging environmental sustainability assessment and process operation at early-stage bioprocess development	22
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	21
Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia	21
Spatial omics techniques and data analysis for cancer immunotherapy applications	21
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	21
Recent developments of oleaginous yeasts toward sustainable biomanufacturing	21
Cell–nano interactions of polydopamine nanoparticles	20
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	20
Cell-free protein synthesis systems for vaccine design and production	20
Sharp solutions to cleave plant fibers	20
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	20
Cutting-edge plant natural product pathway elucidation	20
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	20
Editorial overview: 21st Century solutions for crop improvement	19
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	19
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	19
Advancing fungal biodegradation of nonsteroidal anti-inflammatory drugs — challenges and future perspectives	19
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	19
Artificial intelligence: a solution to involution of design–build–test–learn cycle	19
Bio-integrative design of the neural tissue-device interface	19
Dissecting postharvest chilling injury through biotechnology	19
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	19
Cell-free chemoenzymatic cascades with bio-based molecules	19
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	19
Identification of novel membrane proteins for improved lignocellulose conversion	19
Rational design of peptide-based implants for corneal bioengineering	19
Petrosia ficiformis (Poiret, 1789): an excellent model for holobiont and biotechnological studies	19
The dawn of interoperating spatial models in cell biology	18
Coupling and uncoupling growth and product formation for producing chemicals	18
Editorial Board	18
Biotechnological interventions in reducing losses of tropical fruits and vegetables	18
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	18
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design	18
Harnessing the omics revolution to address the global biodiversity crisis	18
Adaptive biomimicry: design of neural interfaces with enhanced biointegration	18
Coral holobionts and biotechnology: from Blue Economy to coral reef conservation	18
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	18
Recent advances in synthetic glycoengineering for biological applications	18
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	18
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	18
Constructing next-generation CRISPR–Cas tools from structural blueprints	18
AuxSynBio: synthetic biology tools to understand and engineer auxin	17
Disturbance and stability dynamics in microbial communities for environmental biotechnology applications	17
The tier system: a host development framework for bioengineering	17
Advances in mass spectrometry-enabled multiomics at single-cell resolution	17
Advances in genome-scale metabolic models of industrially important fungi	17
Different avenues for progress apply to drought tolerance, water use efficiency and yield in dry areas	17
Transient electronics: new opportunities for implantable neurotechnology	17
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	17
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	17
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	17
Beyond co-expression: pathway discovery for plant pharmaceuticals	16
Mannosylerythritol lipids: production, downstream processing, and potential applications	16
Editorial Board	16
Editorial Board	16
Personalized gut microbial community modeling by leveraging genome-scale metabolic models and metagenomics	16
Biosensor-enabled pathway optimization in metabolic engineering	16
Cyanobacteria as cell factories: the roles of host and pathway engineering and translational research	16
Advancing plant science through precision 3D bioprinting: new tools for research and biotech applications	16
Advances in vascularization and innervation of constructs for neural tissue engineering	16
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	16
Corrigendum to “Advances in engineering substrate scope of Pseudomonas cell factories” [Curr Opin Biotechnol 92 (2025) 103270]	16
Transforming stressed plants into healthy foods	16
Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness	15
Machine learning for synthetic gene circuit engineering	15
Design and fabrication of field-deployable microbial biosensing devices	15
Principles for designing an optimal mRNA lipid nanoparticle vaccine	15
Publisher's Note	15
Multifunctionality of alkaline phosphatase in ecology and biotechnology	15
Diversity: current and prospective secondary metabolites for nutrition and medicine	15
Using systems metabolic engineering strategies for high-oil maize breeding	15
Anti-inflammatory and immunomodulatory properties of polysaccharides in mushrooms	15
Guide RNA library-based CRISPR screens in plants: opportunities and challenges	15
Precision fermentation for improving the quality, flavor, safety, and sustainability of foods	15
Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy	15
CRISPR-based metabolic engineering in non-model microorganisms	15
Carbonic anhydrase for CO2 capture, conversion and utilization	15
Green horizons: how plant synthetic biology can enable space exploration and drive on Earth sustainability	15
Does the surface charge of the nanoparticles drive nanoparticle–cell membrane interactions?	15
Natural products in antibiotic development: is the success story over?	14
Medical properties, market potential, and microbial production of golden polyketide curcumin for food, biomedical, and cosmetic applications	14
Capturing patient anatomy for designing and manufacturing personalized prostheses	14