Current Opinion in Biotechnology

Article	Citations
Microbial electrosynthesis from CO2: forever a promise?	234
Renewable methanol and formate as microbial feedstocks	201
The potential of remote sensing and artificial intelligence as tools to improve the resilience of agriculture production systems	187
Enhancing phytoremediation of soils polluted with heavy metals	123
Biotechnological upcycling of plastic waste and other non-conventional feedstocks in a circular economy	122
Pollution to products: recycling of ‘above ground’ carbon by gas fermentation	119
Machine learning applications in systems metabolic engineering	116
Principles for designing an optimal mRNA lipid nanoparticle vaccine	102
Recent advances in modular co-culture engineering for synthesis of natural products	96
Design and application of oncolytic viruses for cancer immunotherapy	85
T-cell repertoire analysis and metrics of diversity and clonality	80
The future of phage biocontrol in integrated plant protection for sustainable crop production	79
Metagenomic tools in microbial ecology research	78
Unlocking the next generation of phage therapy: the key is in the receptors	76
Engineering probiotics for therapeutic applications: recent examples and translational outlook	75
Large-scale production of enzymes for biotechnology uses	75
Enhancing phage therapy through synthetic biology and genome engineering	72
Microfluidic systems for cancer diagnostics	71
Biotechnological perspectives on algae: a viable option for next generation biofuels	69
Marine collagen scaffolds in tissue engineering	69
Microbial production of polyunsaturated fatty acids — high-value ingredients for aquafeed, superfoods, and pharmaceuticals	64
Cross-linking/mass spectrometry to get a closer view on protein interaction networks	64
Harnessing atmospheric nitrogen for cereal crop production	63
Mixed carbon substrates: a necessary nuisance or a missed opportunity?	62
Sensing the unreachable: challenges and opportunities in biofilm detection	62

Advances in microphysiological blood-brain barrier (BBB) models towards drug delivery	62
Microbial production of extremolytes — high-value active ingredients for nutrition, health care, and well-being	61
Caenorhabditis elegans as a model system for human diseases	61
Microfluidic cultivation and analysis tools for interaction studies of microbial co-cultures	60
Network inference in systems biology: recent developments, challenges, and applications	60
Technoeconomic analysis for biofuels and bioproducts	59
Glutathione and its precursors in cancer	59
Metabolic specialization in itaconic acid production: a tale of two fungi	59
Implantable brain machine interfaces: first-in-human studies, technology challenges and trends	59
Formate dehydrogenases for CO2 utilization	58
Advances in single cell Raman spectroscopy technologies for biological and environmental applications	58
New-to-nature chemistry from old protein machinery: carbene and nitrene transferases	57
Trade-offs between gene expression, growth and phenotypic diversity in microbial populations	57
Recent advances in understanding the ecophysiology of enhanced biological phosphorus removal	56
Transcription factor-based biosensors: a molecular-guided approach for natural product engineering	56
Synthetic microbial consortia for small molecule production	55
Unraveling the mechanobiology of immune cells	55
Bispecific antibodies in cancer immunotherapy	55
RNA-based fluorescent biosensors for live cell imaging of small molecules and RNAs	53
Chain elongators, friends, and foes	53
Single-cell metabolomics: where are we and where are we going?	53
Synthetic co-cultures: novel avenues for bio-based processes	52
The plant microbiota: composition, functions, and engineering	52
Microalgae based production of single-cell protein	51
Current status of pan-genome analysis for pathogenic bacteria	51
Overview of dietary assessment methods for measuring intakes of foods, beverages, and dietary supplements in research studies	51
Fermented food products in the era of globalization: tradition meets biotechnology innovations	51
The role of single cell protein in cellular agriculture	49
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	49
Biobanks for life sciences and personalized medicine: importance of standardization, biosafety, biosecurity, and data management	48
Tracing metabolic flux through time and space with isotope labeling experiments	47
Engineering cyanobacteria chassis cells toward more efficient photosynthesis	47
Circadian rhythms and meal timing: impact on energy balance and body weight	47
Vascularization in skin wound healing: where do we stand and where do we go?	47
Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding	46
Toward sustainable, cell-free biomanufacturing	46
High-throughput screening for improved microbial cell factories, perspective and promise	46
Enzyme evolution for industrial biocatalytic cascades	46
Recent advances on constraint-based models by integrating machine learning	46
New frontiers: harnessing pivotal advances in microbial engineering for the biosynthesis of plant-derived terpenoids	46
Bacteriophage endolysins — extending their application to tissues and the bloodstream	45
Innate immune responses triggered by nucleic acids inspire the design of immunomodulatory nucleic acid nanoparticles (NANPs)	45
Conquering CRISPR: how phages overcome bacterial adaptive immunity	45
RNAs — a new frontier in crop protection	45
Machine learning to navigate fitness landscapes for protein engineering	44
Recent advances in heterologous expression of natural product biosynthetic gene clusters in Streptomyces hosts	44
Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins	44
High-throughput screening for efficient microbial biotechnology	43
Carbonic anhydrase for CO2 capture, conversion and utilization	43
Beyond the semi-synthetic artemisinin: metabolic engineering of plant-derived anti-cancer drugs	43

Optogenetics and biosensors set the stage for metabolic cybergenetics	43
Engineering nanoparticulate vaccines for enhancing antigen cross-presentation	43
Environmental drivers of metabolic heterogeneity in clonal microbial populations	43
Lysins breaking down the walls of Gram-negative bacteria, no longer a no-go	43
The limitless applications of single-cell metabolomics	42
Contemporary aquaculture: implications for human nutrition	42
Modular Analysis and Design of Biological Circuits	40
Patient-derived xenografts (PDXs) as model systems for human cancer	40
A guide to deciphering microbial interactions and metabolic fluxes in microbiome communities	40
Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products	39
Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens	39
Requirements for fungal uptake of dsRNA and gene silencing in RNAi-based crop protection strategies	39
Systems and synthetic biology tools for advanced bioproduction hosts	38
Systems metabolomics: from metabolomic snapshots to design principles	38
Stop helping pathogens: engineering plant susceptibility genes for durable resistance	38
Challenges and opportunities in biological funneling of heterogeneous and toxic substrates beyond lignin	38
Accelerating strain engineering in biofuel research via build and test automation of synthetic biology	38
Multi-chassis engineering for heterologous production of microbial natural products	38
Transcription factor-based biosensors and inducible systems in non-model bacteria: current progress and future directions	37
Targeting biofilms using phages and their enzymes	37
Microbes trading electricity in consortia of environmental and biotechnological significance	37
Complexity of macrophage metabolism in infection	37
Engineering xylose metabolism in yeasts to produce biofuels and chemicals	36
Discovery of new enzymatic functions and metabolic pathways using genomic enzymology web tools	36
Cryoablation and immunotherapy of cancer	36
Biosynthesis of fatty acid-derived hydrocarbons: perspectives on enzymology and enzyme engineering	36
Towards superior plant-based foods using metabolomics	35
Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces	35
Preclinical data and safety assessment of phage therapy in humans	35
Current state of aromatics production using yeast: achievements and challenges	35
Microbial production of fatty acids and derivative chemicals	35
Synthetic biology for protein-based materials	34
The emergence and promise of single-cell temporal-omics approaches	34
Phages to shape the gut microbiota?	34
Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control	34
Enzymes in biotechnology: Critical platform technologies for bioprocess development	34
Metabolic model guided strain design of cyanobacteria	34
Antifungal natural products	33
The engineering of spatially linked microbial consortia – potential and perspectives	33
Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production	33
Application of nanotechnology in medical diagnosis and imaging	33
The ecology and evolution of microbial metabolic strategies	32
Organs-on-a-chip as model systems for multifactorial musculoskeletal diseases	32
Advances in electrochemical cofactor regeneration: enzymatic and non-enzymatic approaches	32
Harnessing ecological and evolutionary principles to guide the design of microbial production consortia	32
Recent progress and advanced technology in carbohydrate-based drug development	32
Biosynthesis of terpene compounds using the non-model yeast Yarrowia lipolytica: grand challenges and a few perspectives	32
TCA cycle signalling and the evolution of eukaryotes	32
Insect sex pheromone production in yeasts and plants	31
Building kinetic models for metabolic engineering	31
Nature’s recyclers: anaerobic microbial communities drive crude biomass deconstruction	31
Advances in G protein-coupled receptor high-throughput screening	31
All systems go: converging synthetic biology and combinatorial treatment for CAR-T cell therapy	31
Recent trends in nanopore polymer functionalization	31
Evolutionary causes and consequences of metabolic division of labour: why anaerobes do and aerobes don’t	31
Phage engineering and the evolutionary arms race	30
Industrial production of microbial protein products	30
Bioconversion of renewable feedstocks by Rhodococcus opacus	30
Time to integrate biotechnological approaches into fish gut microbiome research	30
Status and challenges of electrical stimulation use in chronic wound healing	30
The human gut microbiota during the initial stages of life: insights from bifidobacteria	29
Chitosan and other edible coatings to extend shelf life, manage postharvest decay, and reduce loss and waste of fresh fruits and vegetables	29
Precision medicine — networks to the rescue	29
Omics-based strategies to discover novel classes of RiPP natural products	29
New chemistries and enzymes for synthetic genetics	28
Filamentous fungi for future functional food and feed	28
Recent advances in improving metabolic robustness of microbial cell factories	28
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	28
Challenges and opportunities with CRISPR activation in bacteria for data-driven metabolic engineering	28
Challenges and advantages of cell therapy manufacturing under Good Manufacturing Practices within the hospital setting	28
Metabolic engineering for production of functional polysaccharides	28
RNA nanotechnology in synthetic biology	28
Engineering of ecological niches to create stable artificial consortia for complex biotransformations	27
Diversity: current and prospective secondary metabolites for nutrition and medicine	27
Refactoring biosynthetic gene clusters for heterologous production of microbial natural products	27
Cascaded valorization of seaweed using microbial cell factories	27
Engineering triacylglycerol production from sugars in oleaginous yeasts	26
Engineering of natural product biosynthesis in Pseudomonas putida	26
Engineering microbial cell morphology and membrane homeostasis toward industrial applications	26
Incorporating biological structure into machine learning models in biomedicine	26

Beyond antibacterials – exploring bacteriophages as antivirulence agents	26
Phage-specific antibodies	26
Engineering commensal bacteria to rewire host–microbiome interactions	26
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	26
Synthetic metabolism for biohalogenation	26
Resolving complex phenotypes with Raman spectroscopy and chemometrics	26
Bacillus spore awakening: recent discoveries and technological developments	25
Metabolic interactions in beneficial microbe recruitment by plants	25
Quantitative analysis of glycine related metabolic pathways for one-carbon synthetic biology	25
The sum is greater than the parts: exploiting microbial communities to achieve complex functions	25
Assessing the potential to harness the microbiome through plant genetics	25
Biotechnologies to tackle the challenge of neoantigen identification	25
Aptamers in RNA-based switches of gene expression	25
Combining protein and metabolic engineering to construct efficient microbial cell factories	25
Biosensor-enabled pathway optimization in metabolic engineering	25
The role of cuticle in fruit shelf-life	25
Enzymatic control of product distribution in terpene synthases: insights from multiscale simulations	25
Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness	25
Cyanobacteria as cell factories: the roles of host and pathway engineering and translational research	25
Receptor kinases in plant responses to herbivory	24
Gene delivery for immunoengineering	24
Engineering long-circulating nanomaterial delivery systems	24
Unlocking nature’s biosynthetic potential by directed genome evolution	24
The role of systems biology in developing non-model cyanobacteria as hosts for chemical production	24
Chassis engineering for microbial production of chemicals: from natural microbes to synthetic organisms	24
Native LC–MS for capturing quality attributes of biopharmaceuticals on the intact protein level	24
Siderophore natural products as pharmaceutical agents	23
Recent advances in bivalve-microbiota interactions for disease prevention in aquaculture	23
Biotechnology for secure biocontainment designs in an emerging bioeconomy	23
CO2-converting enzymes for sustainable biotechnology: from mechanisms to application	23
Delivery of CRISPR-Cas systems using phage-based vectors	23
Aspergillus terreus as an industrial filamentous fungus for pharmaceutical biotechnology	23
Virus-induced metabolic reprogramming and innate sensing hereof by the infected host	23
Genetically-encoded biosensors for analyzing and controlling cellular process in yeast	23
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	23
Utilizing cross-species co-cultures for discovery of novel natural products	22
Organoid technology and applications in cancer immunotherapy and precision medicine	22
A roadmap to engineering antiviral natural products synthesis in microbes	22
Engineering microbial consortia with rationally designed cellular interactions	22
Neural interface systems with on-device computing: machine learning and neuromorphic architectures	22
Molecular characterization of HEK293 cells as emerging versatile cell factories	22
Strategies for genotype-flexible plant transformation	21
Engineering of citrus to obtain huanglongbing resistance	21
Synthetic molecular evolution of antimicrobial peptides	21
Can recombinant milk proteins replace those produced by animals?	21
Probiotics for coral aquaculture: challenges and considerations	21
New synthetic biology tools for metabolic control	21
Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development	21
CRISPR-based metabolic engineering in non-model microorganisms	21
Phage Biobank: Present Challenges and Future Perspectives	21
Design and engineering of artificial microbial consortia for biohydrogen production	21
Dissecting postharvest chilling injury through biotechnology	21
Engineering biology approaches for food and nutrient production by cyanobacteria	21
Understanding the impact of age-related changes in the gut microbiome on chronic diseases and the prospect of elderly-specific dietary interventions	21
Polyketide pesticides from actinomycetes	21
Modeling of interspecies electron transfer in anaerobic microbial communities	21
Engineering the plant microbiota in the context of the theory of ecological communities	21
Enhancing enzymatic performance with nanoparticle immobilization: improved analytical and control capability for synthetic biochemistry	21
SpaceHort: redesigning plants to support space exploration and on-earth sustainability	21
Calling for a systems approach in microbiome research and innovation	21
Metabolic regulation of epigenetic remodeling in immune cells	21
Engineering microbial diagnostics and therapeutics with smart control	21
Engineered biomaterials for heart disease	20
Engineered bacterial microcompartments: apps for programming metabolism	20
Biosynthesis of human milk oligosaccharides via metabolic engineering approaches: current advances and challenges	20
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	20
T cell metabolism in homeostasis and cancer immunity	20
Transient electronics: new opportunities for implantable neurotechnology	20
Phage-based target discovery and its exploitation towards novel antibacterial molecules	20
Metabolic engineering strategies to overcome precursor limitations in isoprenoid biosynthesis	20
Heterologous expression and metabolic engineering tools for improving terpenoids production	20
Division of labor for substrate utilization in natural and synthetic microbial communities	20
Strategies for engineering improved nitrogen use efficiency in crop plants via redistribution and recycling of organic nitrogen	20
Looking into the future of phage-based control of zoonotic pathogens in food and animal production	19
Synthetic or natural? Metabolic engineering for assimilation and valorization of methanol	19
Bacterial metabolic heterogeneity: origins and applications in engineering and infectious disease	19
Innovative approaches for the processes involved in microbial biodeterioration of cultural heritage materials	19
Integration of omics data to unravel root microbiome recruitment	19
Yeast based biorefineries for oleochemical production	19
Enzymatic upgrading of heteroxylans for added-value chemicals and polymers	19
Emerging concepts in bone repair and the premise of soft materials	19
Compartmentalized biosynthesis of fungal natural products	19
Mediated electrochemistry for redox-based biological targeting: entangling sensing and actuation for maximizing information transfer	19
Synthetic mRNA – emerging new class of drug for tissue regeneration	19
Heterologous biosynthesis as a platform for producing new generation natural products	18
Phenotypic heterogeneity of microbial populations under nutrient limitation	18
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	18
Recent progress in the synthesis of advanced biofuel and bioproducts	18
Successful delivery of nutrition programs and the sustainable development goals	18
Manufactured extracellular vesicles as human therapeutics: challenges, advances, and opportunities	18
Barriers to microalgal mass cultivation	18
Metabolic flux analysis and fluxomics-driven determination of reaction free energy using multiple isotopes	18
Engineering immunogenic cell death with nanosized drug delivery systems improving cancer immunotherapy	18
Beyond calorie restriction: aging as a biological target for nutrient therapies	18
Advances in quantifying circulatory microRNA for early disease detection	18