Current Opinion in Biotechnology

Article	Citations
Editorial Board	159
Editorial Board	148
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	134
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	110
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	109
Creating value from purple phototrophic bacteria via single-cell protein production	105
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	96
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	93
Systems biology-based analysis of cell-free systems	91
Recent progress in the synthesis of advanced biofuel and bioproducts	91
Anaerobic microbial methanol utilization as a one-carbon feedstock	87
Bacterial degradation of emerging aromatic pollutants and integrated strategies for sustainable healthy agro-ecosystem	78
Cell-free technologies for biopharmaceutical research and production	75
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	75
Deep learning in regulatory genomics: from identification to design	69
A molecular assessment of the practical potential of DNA-based computation	68
Engineered T cells and macrophages: two arms to seize solid tumors	67
Coupling chemistry and biology for the synthesis of advanced bioproducts	67
Emerging technologies for genetic control systems in cellular therapies	66
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	65
Editorial Board	65
Editorial Board	63
Engineered living bioassemblies for biomedical and functional material applications	62
Lighting the way: recent developments and applications in molecular optogenetics	59
Innovations and trends in antibody repertoire analysis	59

Microfluidics meets cell-free systems: from molecular engineering to synthetic cells	57
Single-cell analysis opens a goldmine for plant functional studies	57
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	57
Expanding the catalytic repertoire of C–C bond–forming pyridoxal 5′-phosphate-dependent enzymes for noncanonical amino acid formation	57
Molecular methods for high-throughput, multiplexed, and automated genome editing in prokaryotes and eukaryotes	56
Strategies and challenges for synthetic apomixis	56
Multifaceted metabolic role of infections in the tumor microenvironment	56
Dietary microalgae on poultry meat and eggs: explained versus unexplained effects	55
Computational tools and resources for designing new pathways to small molecules	55
Photoprotective-based strategies to enhance crop yield under fluctuating light conditions	54
Strategies for engineering domesticated and undomesticated human microbes	53
Deciphering the guanidine code: from core metabolism to biosynthesis of its derivatives	53
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	51
Using genome and transcriptome analysis to elucidate biosynthetic pathways	51
Engineering of the genetic code	51
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	50
Mass spectrometry imaging methods for visualizing tumor heterogeneity	49
2-hydroxyglutarate rides the cancer-immunity cycle	49
Machine learning to navigate fitness landscapes for protein engineering	48
Perspectives for self-driving labs in synthetic biology	48
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	48
Editorial Board	47
Advancing in vivo reprogramming with synthetic biology	45
Harnessing the potential of microbial methane utilization for chasing sustainability	45
Editorial Board	45
Toolboxes for plant systems biology research	45
Matching diverse feedstocks to conversion processes for the future bioeconomy	45
Plant synthetic biology as a tool to help eliminate hidden hunger	44
Reducing crop losses by gene-editing control of organ developmental physiology	43
Building trust in automated experimentation: uncertainty quantification in the era of high-throughput biolabs	43
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	42
Synthetic biology approaches for dynamic CHO cell engineering	42
Low-cost, point-of-care biomarker quantification	41
Understanding atmospheric intercontinental dispersal of harmful microorganisms	40
Editorial overview: Pioneering innovations in food biotechnology	40
Impact of anthropogenic contamination on glacier surface biota	40
The potential of amoeba-based processes for natural product syntheses	39
Sourcing DNA parts for synthetic biology applications in plants	38
Plant microbiome regulation for sustainable agriculture	38
Metabolic and cellular engineering for the production of natural products	38
Pathway engineering for the biosynthesis of psychedelics	38
Molecules to medicine: advances in metabolomics for natural product drug discovery	37
Synthetic apomixis: the beginning of a new era	37
Single-cell omics analysis with genome-scale metabolic modeling	37
Biochemical production with microbial bioelectrochemical systems	37
Editorial overview: Nanobiotechnology for immunoengineering	36
PRRs and NLRs sans frontières: advances and challenges in transfer of immune receptors between plant species	36
Editorial Board	36
Complexity of subcellular metabolism: strategies for compartment-specific profiling	35
What’s new in single-cell proteomics	35

Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes	35
Extracellular vesicles as therapeutics for inflammation and infection	35
Matchmaking at the cell surface using bispecifics to put cells on their best behavior	34
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	34
Potential use of microbial engineering in single-cell protein production	33
Synthetic fuels: what are they and where do they come from?	33
Next-generation biological vector platforms for in vivo delivery of genome editing agents	33
Understanding and harnessing the complexity of interspecies interactions in acetogenic mixotrophic co-cultures	32
The aging tumor metabolic microenvironment	32
Engineered methane biocatalysis: strategies to assimilate methane for chemical production	32
Targeting ripening regulators to develop fruit with high quality and extended shelf life	32
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	31
Programmable synthetic biology tools for developing microbial cell factories	31
Editorial Board	31
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	31
Recent innovations in cannabinoid chemistry, biology, and biosynthesis	30
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	30
Editorial Board	30
Engineering whole-cell catalysts to use plastic waste as a feedstock	30
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	30
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	30
De novo domestication: retrace the history of agriculture to design future crops	30
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	30
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	29
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	29
Can digital twin efforts shape microorganism-based alternative food?	29
Recent advances in microbial lignin degradation and upcycling of lignin-derived aromatic compounds	29
Strategies for uncovering stable isotope tracing patterns between cell populations	28
Progress and challenges in the translation of cancer nanomedicines	28
Machine learning for predicting phenotype from genotype and environment	28
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	28
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	28
In situ characterization of the tumor microenvironment	27
Editorial overview: Tissue, cell, and pathway engineering	27
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	27
Division of labor for substrate utilization in natural and synthetic microbial communities	27
Mycotoxin risks are lower in biotech corn	27
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	27
Senescent cells in tissue engineering	27
Design of DNA strand displacement reactions	27
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	27
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	27
Editorial Board	27
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	26
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	26
Engineering plant–microbe communication for plant nutrient use efficiency	26
Sustainability in food-waste reduction biotechnology: a critical review	26
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	26
Advances in engineering substrate scope of Pseudomonas cell factories	26
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	25
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	25
Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	25
Recent advances in microbial production of rare sugars and steviols	25
Eubacterium limosum as a biocatalyst for sustainable methanol bioconversion	25
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	25
Two in one: the emerging concept of bifunctional antibodies	25
Engineering bacteria for cancer immunotherapy	24
Advances in artificial biosynthesis of bioactive plant natural products	24
Advances in engineering algae for biofuel production	24
Computational models in systems biology: standards, dissemination, and best practices	24
Recent advances in microbial synthesis of polyphenols	24
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	24
Designed dietary fibers: engineering carbohydrate structure for precision modulation of the human gut microbiome	23
Drugging the microbiome and bacterial live biotherapeutic consortium production	23
Carbon recycling with synthetic CO2 fixation pathways	23
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	23
Building mutational bridges between carbohydrate-active enzymes	23
Understanding methanol metabolism through systems biology: advances and future perspectives	23
Reprogramming immunity: TAL effector-informed genome editing in rice and other crops	23
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	22
Shaping Aspergillus niger into a next-generation cell factory for L-malic acid	22
Quantifying nanoparticle delivery: challenges, tools, and advances	22
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	22
Biomolecular feedback controllers: from theory to applications	22
Engineering organoids as cerebral disease models	22
Cell-free biosensors: where have we been and where do we need to go?	22
Deep learning and generative artificial intelligence methods in enzyme and cell engineering	22
Engineering microbial consortia with rationally designed cellular interactions	22
Architectures of emerging biofoundry platforms for synthetic biology	22
Cell-free enzyme cascades — application and transition from development to industrial implementation	22

Approaches for a more microbiologically and chemically safe dried fruit supply chain	22
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	22
Anaerobic microbial core for municipal wastewater treatment — the sustainable platform for resource recovery	21
Editorial overview: Phosphate Special Issue	21
Genetically pliable green algae for bioproduction of modified fatty acids, nutritional therapeutic oils, and biopharmaceuticals	21
Machine learning in wastewater: opportunities and challenges — “not everything is a nail!”	21
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	21
Filamentous fungi for future functional food and feed	21
Unlocking therapeutic impacts of the gut microbiota with computational tools	21
Technologies for the discovery of G protein–coupled receptor–targeting biologics	21
Resolving misconceptions and constraints in growth-coupled bioproduction	21
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	21
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	21
The double life of CRISPR–Cas13	21
Optogenetics in medicine: innovations and therapeutic applications	21
A guide to designing cell-surface receptors in plants	21
SgRNA engineering for improved genome editing and expanded functional assays	21
Continuous biomanufacturing with microbes — upstream progresses and challenges	21
Molecular biotechnology for the biodegradation of organofluorine compounds	21
Window of sustainable bioprocess operation: towards merging environmental sustainability assessment and process operation at early-stage bioprocess development	21
Editorial overview: 21st Century solutions for crop improvement	20
Recent developments of oleaginous yeasts toward sustainable biomanufacturing	20
Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia	20
Sharp solutions to cleave plant fibers	20
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	20
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	20
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	20
Cell-free protein synthesis systems for vaccine design and production	20
Cutting-edge plant natural product pathway elucidation	20
Rational design of peptide-based implants for corneal bioengineering	20
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	20
Controlled protein synthesis and spatial organisation in microfluidic environments	19
Advancing fungal biodegradation of nonsteroidal anti-inflammatory drugs — challenges and future perspectives	19
Cell-free chemoenzymatic cascades with bio-based molecules	19
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	19
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	19
Engineered microbial living matter for diagnostics, prevention, and therapy	19
Technoeconomic analysis for near-term scale-up of bioprocesses	19
The dawn of interoperating spatial models in cell biology	18
Dissecting postharvest chilling injury through biotechnology	18
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	18
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	18
Cell–nano interactions of polydopamine nanoparticles	18
Artificial intelligence: a solution to involution of design–build–test–learn cycle	18
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	18
Biotechnological interventions in reducing losses of tropical fruits and vegetables	18
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	18
Spatial omics techniques and data analysis for cancer immunotherapy applications	18
Constructing next-generation CRISPR–Cas tools from structural blueprints	17
Coupling and uncoupling growth and product formation for producing chemicals	17
Editorial Board	17
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	17
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design	17
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	16
The tier system: a host development framework for bioengineering	16
Mannosylerythritol lipids: production, downstream processing, and potential applications	16
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	16
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	16
Harnessing the omics revolution to address the global biodiversity crisis	16
AuxSynBio: synthetic biology tools to understand and engineer auxin	16
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	16
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	16
Advances in mass spectrometry-enabled multiomics at single-cell resolution	15
Advances in genome-scale metabolic models of industrially important fungi	15
Transforming stressed plants into healthy foods	15
Beyond co-expression: pathway discovery for plant pharmaceuticals	15
Large language model agents as experimental orchestrators in data-driven enzyme engineering	15
Editorial Board	15
Corrigendum to “Advances in engineering substrate scope of Pseudomonas cell factories” [Curr Opin Biotechnol 92 (2025) 103270]	15
Editorial Board	15
Emerging strategies to enhance microbial natural product–based drug discovery	15
Biosensor-enabled pathway optimization in metabolic engineering	15
Disturbance and stability dynamics in microbial communities for environmental biotechnology applications	15
Using systems metabolic engineering strategies for high-oil maize breeding	15
CRISPR-based metabolic engineering in non-model microorganisms	14
Precision fermentation for improving the quality, flavor, safety, and sustainability of foods	14
Personalized gut microbial community modeling by leveraging genome-scale metabolic models and metagenomics	14
Anti-inflammatory and immunomodulatory properties of polysaccharides in mushrooms	14
Design and fabrication of field-deployable microbial biosensing devices	14
Advancing plant science through precision 3D bioprinting: new tools for research and biotech applications	14
Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy	14
Ionizable lipids empowered with artificial intelligence for mRNA delivery	14
Publisher's Note	13
Multifunctionality of alkaline phosphatase in ecology and biotechnology	13
Starch and sugars as determinants of postharvest shelf life and quality: some new and surprising roles	13
Effects of sewage sludge ash as a recycled phosphorus source on the soil microbiome	13
From toxin to biofuel: engineering microbes for methanol biomanufacturing	13
Guide RNA library-based CRISPR screens in plants: opportunities and challenges	13
Overcoming barriers to medium-chain fatty alcohol production	13
Tools for automated genome editing of stem cells	13
Machine learning for synthetic gene circuit engineering	13
Discovery and development of penicillin-binding protein-type thioesterases as biocatalysts	13
Corrigendum to: “Advances in consolidated bioprocessing using synthetic cellulosomes” [Curr Opin Biotechnol 2022, 78:102840]	13
Genome mining strategies for metallophore discovery	13
Green horizons: how plant synthetic biology can enable space exploration and drive on Earth sustainability	13