Current Opinion in Biotechnology

Article	Citations
Editorial Board	259
Editorial Board	201
Stem cells characterization: OMICS reinforcing analytics	120
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	120
Systems biology-based analysis of cell-free systems	110
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	108
Anaerobic microbial methanol utilization as a one-carbon feedstock	102
Recent progress in the synthesis of advanced biofuel and bioproducts	97
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	97
Bioelectrosynthesis systems	93
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	90
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	90
Cell-free technologies for biopharmaceutical research and production	87
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	86
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	83
Creating value from purple phototrophic bacteria via single-cell protein production	78
Editorial Board	66
A molecular assessment of the practical potential of DNA-based computation	65
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	65
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	63
Engineered T cells and macrophages: two arms to seize solid tumors	63
The plant microbiota: composition, functions, and engineering	60
Bridging the gap — biomimetic design of bioelectronic interfaces	58
Coupling chemistry and biology for the synthesis of advanced bioproducts	55
Lighting the way: recent developments and applications in molecular optogenetics	54

Deep learning in regulatory genomics: from identification to design	53
Leveraging rAAV bioprocess understanding and next generation bioanalytics development	52
Emerging technologies for genetic control systems in cellular therapies	51
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	51
Innovations and trends in antibody repertoire analysis	51
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	50
Engineered living bioassemblies for biomedical and functional material applications	50
2-hydroxyglutarate rides the cancer-immunity cycle	50
Single-cell analysis opens a goldmine for plant functional studies	50
Engineering of the genetic code	48
Computational tools and resources for designing new pathways to small molecules	47
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	47
Dietary microalgae on poultry meat and eggs: explained versus unexplained effects	47
Using genome and transcriptome analysis to elucidate biosynthetic pathways	46
Mass spectrometry imaging methods for visualizing tumor heterogeneity	45
Multifaceted metabolic role of infections in the tumor microenvironment	45
Perspectives for self-driving labs in synthetic biology	44
Machine learning to navigate fitness landscapes for protein engineering	44
Editorial Board	42
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	42
Editorial overview: Biotechnological insights of marine and terrestrial microbiome	42
Toolboxes for plant systems biology research	42
Advancing in vivo reprogramming with synthetic biology	41
Matching diverse feedstocks to conversion processes for the future bioeconomy	41
Plant synthetic biology as a tool to help eliminate hidden hunger	41
Reducing crop losses by gene-editing control of organ developmental physiology	41
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	40
Anaerobic fluorescent reporters for cell identification, microbial cell biology and high-throughput screening of microbiota and genomic libraries	40
The potential of amoeba-based processes for natural product syntheses	40
Harnessing the potential of microbial methane utilization for chasing sustainability	40
Analytics of host cell proteins (HCPs): lessons from biopharmaceutical mAb analysis for Gene therapy products	40
Sourcing DNA parts for synthetic biology applications in plants	39
Editorial overview: Emerging routes to sustainable energy capture and conversion into value-added products	39
Redox as a bioprocess parameter: analytical redox quantification in biological therapeutic production	39
Single-cell omics analysis with genome-scale metabolic modeling	38
Synthetic biology approaches for dynamic CHO cell engineering	38
Metabolic and cellular engineering for the production of natural products	38
Synthetic apomixis: the beginning of a new era	37
Low-cost, point-of-care biomarker quantification	37
Pathway engineering for the biosynthesis of psychedelics	37
Understanding atmospheric intercontinental dispersal of harmful microorganisms	37
Editorial overview: Pioneering innovations in food biotechnology	37
Editorial overview: Nanobiotechnology for immunoengineering	36
Impact of anthropogenic contamination on glacier surface biota	36
Editorial Board	36
Biochemical production with microbial bioelectrochemical systems	36
The aging tumor metabolic microenvironment	35
Editorial Board	35
Matchmaking at the cell surface using bispecifics to put cells on their best behavior	35
Complexity of subcellular metabolism: strategies for compartment-specific profiling	35

Antigen-specific nanomedicines for the treatment of autoimmune disease: target cell types, mechanisms and outcomes	35
Synthetic fuels: what are they and where do they come from?	35
Targeting ripening regulators to develop fruit with high quality and extended shelf life	35
Engineered methane biocatalysis: strategies to assimilate methane for chemical production	35
Synthetic mRNA – emerging new class of drug for tissue regeneration	35
What’s new in single-cell proteomics	35
Extracellular vesicles as therapeutics for inflammation and infection	34
Understanding and harnessing the complexity of interspecies interactions in acetogenic mixotrophic co-cultures	34
Next-generation biological vector platforms for in vivo delivery of genome editing agents	33
Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes	33
Potential use of microbial engineering in single-cell protein production	33
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	33
Programmable synthetic biology tools for developing microbial cell factories	33
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	32
Editorial Board	32
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	32
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	32
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	32
Strategies for uncovering stable isotope tracing patterns between cell populations	32
De novo domestication: retrace the history of agriculture to design future crops	31
Biohybrid approaches to interface with the nervous system: the best of both worlds	31
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	31
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	31
Can digital twin efforts shape microorganism-based alternative food?	31
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	31
Editorial overview: Tissue, cell, and pathway engineering	30
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	30
Inhalable mRNA vaccines for respiratory diseases: a roadmap	30
Editorial Board	30
Machine learning for predicting phenotype from genotype and environment	30
Practical monitoring technologies for cells and substrates in biomanufacturing	30
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	29
Corrigendum to “Unraveling the mechanobiology of immune cells” [Curr Opin Biotechnol 66 (2020) 236-245]	29
Senescent cells in tissue engineering	29
Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks	29
Editorial Board	29
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	28
In situ characterization of the tumor microenvironment	28
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	28
Progress and challenges in the translation of cancer nanomedicines	28
Mycotoxin risks are lower in biotech corn	28
Sustainability in food-waste reduction biotechnology: a critical review	28
Two in one: the emerging concept of bifunctional antibodies	28
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	27
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	27
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	27
Advances in engineering substrate scope of Pseudomonas cell factories	27
General framework for enzyme-photo-coupled catalytic system toward carbon dioxide conversion	27
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	26
Engineering plant–microbe communication for plant nutrient use efficiency	26
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	26
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	26
Division of labor for substrate utilization in natural and synthetic microbial communities	25
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	25
Force in numbers: high-throughput screening approaches to unlock microbial transport	25
Recent advances in microbial production of rare sugars and steviols	25
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	25
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	25
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	25
Recent advances in microbial synthesis of polyphenols	24
Chemical speciation of trace metals in mammalian cell culture media: looking under the hood to boost cellular performance and product quality	24
Carbon recycling with synthetic CO2 fixation pathways	24
Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	24
Advances in engineering algae for biofuel production	24
Computational models in systems biology: standards, dissemination, and best practices	24
Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics	24
Engineering bacteria for cancer immunotherapy	24
Drugging the microbiome and bacterial live biotherapeutic consortium production	23
Anaerobic microbial core for municipal wastewater treatment — the sustainable platform for resource recovery	23
Cytochromes P450 in the biocatalytic valorization of lignin	23
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	23
Cellobiose dehydrogenase in biofuel cells	23
Quantifying nanoparticle delivery: challenges, tools, and advances	23
In vivo 2H/13C flux analysis in metabolism research	23
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	23
Engineering organoids as cerebral disease models	23
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	23
Approaches for a more microbiologically and chemically safe dried fruit supply chain	22
Cell-free enzyme cascades — application and transition from development to industrial implementation	22
Building mutational bridges between carbohydrate-active enzymes	22

Engineering microbial consortia with rationally designed cellular interactions	22
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	22
Editorial Board	22
Continuous biomanufacturing with microbes — upstream progresses and challenges	22
Biomolecular feedback controllers: from theory to applications	22
Window of sustainable bioprocess operation: towards merging environmental sustainability assessment and process operation at early-stage bioprocess development	21
Filamentous fungi for future functional food and feed	21
Machine learning in wastewater: opportunities and challenges — “not everything is a nail!”	21
Technologies for the discovery of G protein–coupled receptor–targeting biologics	21
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	21
Editorial overview: Tissue, cell and pathway engineering: bioinspired, biomimetic and hybrid neural interfaces	21
Editorial overview: Phosphate Special Issue	21
The double life of CRISPR–Cas13	21
Optogenetics in medicine: innovations and therapeutic applications	21
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	20
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	20
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	20
Time to integrate biotechnological approaches into fish gut microbiome research	20
Microbial production of chemicals driven by CRISPR-Cas systems	20
SgRNA engineering for improved genome editing and expanded functional assays	20
Editorial overview: 21st Century solutions for crop improvement	20
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	19
Sharp solutions to cleave plant fibers	19
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	19
Advancing fungal biodegradation of nonsteroidal anti-inflammatory drugs — challenges and future perspectives	19
Cutting-edge plant natural product pathway elucidation	19
Surveilling cellular vital signs: toward label-free biosensors and real-time viability assays for bioprocessing	19
Engineered microbial living matter for diagnostics, prevention, and therapy	19
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	19
Cell-free protein synthesis systems for vaccine design and production	19
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	19
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	19
Technoeconomic analysis for near-term scale-up of bioprocesses	19
Spatial omics techniques and data analysis for cancer immunotherapy applications	19
Recent developments of oleaginous yeasts toward sustainable biomanufacturing	19
Petrosia ficiformis (Poiret, 1789): an excellent model for holobiont and biotechnological studies	18
Cell–nano interactions of polydopamine nanoparticles	18
Cell-free chemoenzymatic cascades with bio-based molecules	18
Dissecting postharvest chilling injury through biotechnology	18
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	18
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	18
Artificial intelligence: a solution to involution of design–build–test–learn cycle	18
Identification of novel membrane proteins for improved lignocellulose conversion	18
Bio-integrative design of the neural tissue-device interface	18
Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia	18
Rational design of peptide-based implants for corneal bioengineering	18
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	18
Coupling and uncoupling growth and product formation for producing chemicals	17
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design	17
Adaptive biomimicry: design of neural interfaces with enhanced biointegration	17
The dawn of interoperating spatial models in cell biology	17
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	17
Editorial Board	17
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	17
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	17
Constructing next-generation CRISPR–Cas tools from structural blueprints	17
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	17
Biotechnological interventions in reducing losses of tropical fruits and vegetables	17
Transdermal sensing: in-situ non-invasive techniques for monitoring of human biochemical status	17
Disturbance and stability dynamics in microbial communities for environmental biotechnology applications	16
Recent advances in synthetic glycoengineering for biological applications	16
AuxSynBio: synthetic biology tools to understand and engineer auxin	16
Cyanobacteria as cell factories: the roles of host and pathway engineering and translational research	16
Mannosylerythritol lipids: production, downstream processing, and potential applications	16
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	16
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	16
Transient electronics: new opportunities for implantable neurotechnology	16
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	16
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	16
Harnessing the omics revolution to address the global biodiversity crisis	16
Coral holobionts and biotechnology: from Blue Economy to coral reef conservation	16
The tier system: a host development framework for bioengineering	15
Editorial Board	15
Corrigendum to “Advances in engineering substrate scope of Pseudomonas cell factories” [Curr Opin Biotechnol 92 (2025) 103270]	15
Advances in vascularization and innervation of constructs for neural tissue engineering	15
Carbonic anhydrase for CO2 capture, conversion and utilization	15
Personalized gut microbial community modeling by leveraging genome-scale metabolic models and metagenomics	15
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	15
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	15
Editorial Board	15
Advances in mass spectrometry-enabled multiomics at single-cell resolution	15
Machine learning for synthetic gene circuit engineering	15
Different avenues for progress apply to drought tolerance, water use efficiency and yield in dry areas	15
Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness	15
Biosensor-enabled pathway optimization in metabolic engineering	15
Advances in genome-scale metabolic models of industrially important fungi	15
Transforming stressed plants into healthy foods	15
Anti-inflammatory and immunomodulatory properties of polysaccharides in mushrooms	15
Beyond co-expression: pathway discovery for plant pharmaceuticals	15
Diversity: current and prospective secondary metabolites for nutrition and medicine	15
Effects of sewage sludge ash as a recycled phosphorus source on the soil microbiome	14
Publisher's Note	14
Corrigendum to: “Advances in consolidated bioprocessing using synthetic cellulosomes” [Curr Opin Biotechnol 2022, 78:102840]	14
Green horizons: how plant synthetic biology can enable space exploration and drive on Earth sustainability	14
Multifunctionality of alkaline phosphatase in ecology and biotechnology	14