Current Opinion in Biotechnology

Article	Citations
Editorial Board	169
Editorial Board	149
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	134
Anaerobic microbial methanol utilization as a one-carbon feedstock	115
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	111
Bacterial degradation of emerging aromatic pollutants and integrated strategies for sustainable healthy agro-ecosystem	105
Recent progress in the synthesis of advanced biofuel and bioproducts	100
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	97
Creating value from purple phototrophic bacteria via single-cell protein production	95
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	91
Spatial enzyme assembly for microbial pharmaceutical synthesis	89
Cytochrome P450-mediated structural diversification of diketopiperazine alkaloids	83
Advances in synthetic microbial consortia for improved production of biochemicals and biofuels	80
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	79
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	72
Cell-free technologies for biopharmaceutical research and production	72
A molecular assessment of the practical potential of DNA-based computation	69
Systems biology-based analysis of cell-free systems	69
Editorial Board	68
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	67
Innovations and trends in antibody repertoire analysis	65
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	65
Deep learning in regulatory genomics: from identification to design	65
Editorial Board	61
Engineered T cells and macrophages: two arms to seize solid tumors	60

Expanding the catalytic repertoire of C–C bond–forming pyridoxal 5′-phosphate-dependent enzymes for noncanonical amino acid formation	60
Coupling chemistry and biology for the synthesis of advanced bioproducts	59
Single-cell analysis opens a goldmine for plant functional studies	58
Lighting the way: recent developments and applications in molecular optogenetics	58
Metabolic engineering of microorganisms for the production of fatty acid-derived biofuels	57
Molecular methods for high-throughput, multiplexed, and automated genome editing in prokaryotes and eukaryotes	57
Strategies and challenges for synthetic apomixis	57
Emerging technologies for genetic control systems in cellular therapies	57
Engineered living bioassemblies for biomedical and functional material applications	57
Microfluidics meets cell-free systems: from molecular engineering to synthetic cells	56
Multifaceted metabolic role of infections in the tumor microenvironment	55
Engineering of the genetic code	54
Dietary microalgae on poultry meat and eggs: explained versus unexplained effects	54
Computational tools and resources for designing new pathways to small molecules	53
2-hydroxyglutarate rides the cancer-immunity cycle	52
Mass spectrometry imaging methods for visualizing tumor heterogeneity	51
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	51
Photoprotective-based strategies to enhance crop yield under fluctuating light conditions	50
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	50
Deciphering the guanidine code: from core metabolism to biosynthesis of its derivatives	50
Machine learning to navigate fitness landscapes for protein engineering	49
Using genome and transcriptome analysis to elucidate biosynthetic pathways	49
Strategies for engineering domesticated and undomesticated human microbes	48
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	48
Perspectives for self-driving labs in synthetic biology	48
Editorial Board	47
Toolboxes for plant systems biology research	47
Matching diverse feedstocks to conversion processes for the future bioeconomy	46
Editorial Board	44
Advancing in vivo reprogramming with synthetic biology	43
Plant synthetic biology as a tool to help eliminate hidden hunger	43
Reducing crop losses by gene-editing control of organ developmental physiology	42
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	41
Building trust in automated experimentation: uncertainty quantification in the era of high-throughput biolabs	41
Editorial overview: Pioneering innovations in food biotechnology	41
Advances in multiplex precision genome editing in eukaryotic and prokaryotic systems	41
Understanding atmospheric intercontinental dispersal of harmful microorganisms	41
Harnessing the potential of microbial methane utilization for chasing sustainability	41
Pathway engineering for the biosynthesis of psychedelics	40
Single-cell omics analysis with genome-scale metabolic modeling	40
Metabolic and cellular engineering for the production of natural products	40
Impact of anthropogenic contamination on glacier surface biota	39
Sourcing DNA parts for synthetic biology applications in plants	38
Plant microbiome regulation for sustainable agriculture	38
The potential of amoeba-based processes for natural product syntheses	38
Synthetic apomixis: the beginning of a new era	38
Biochemical production with microbial bioelectrochemical systems	37
Molecules to medicine: advances in metabolomics for natural product drug discovery	37
Synthetic biology approaches for dynamic CHO cell engineering	37
Low-cost, point-of-care biomarker quantification	37

PRRs and NLRs sans frontières: advances and challenges in transfer of immune receptors between plant species	37
Editorial overview: Nanobiotechnology for immunoengineering	36
Extracellular vesicles as therapeutics for inflammation and infection	35
Editorial Board	35
Matchmaking at the cell surface using bispecifics to put cells on their best behavior	34
Potential use of microbial engineering in single-cell protein production	34
Understanding and harnessing the complexity of interspecies interactions in acetogenic mixotrophic co-cultures	34
Targeting ripening regulators to develop fruit with high quality and extended shelf life	33
Complexity of subcellular metabolism: strategies for compartment-specific profiling	33
Programmable synthetic biology tools for developing microbial cell factories	33
Engineered methane biocatalysis: strategies to assimilate methane for chemical production	33
Next-generation biological vector platforms for in vivo delivery of genome editing agents	32
Mind the gap: the challenges and opportunities for genomics-driven harnessing of plant metabolic diversity for therapeutic applications	32
The aging tumor metabolic microenvironment	32
Synthetic fuels: what are they and where do they come from?	32
What’s new in single-cell proteomics	32
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	32
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	31
Editorial Board	31
Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes	31
Engineering whole-cell catalysts to use plastic waste as a feedstock	31
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	31
Editorial Board	31
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	31
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	31
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	30
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	30
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	30
Can digital twin efforts shape microorganism-based alternative food?	29
Strategies for uncovering stable isotope tracing patterns between cell populations	29
Machine learning for predicting phenotype from genotype and environment	29
Recent advances in microbial lignin degradation and upcycling of lignin-derived aromatic compounds	29
Non-agricultural feedstocks for next-generation biomanufacturing with yeasts	29
Recent innovations in cannabinoid chemistry, biology, and biosynthesis	28
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	28
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	28
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	28
Two in one: the emerging concept of bifunctional antibodies	28
De novo domestication: retrace the history of agriculture to design future crops	28
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	28
Engineering plant–microbe communication for plant nutrient use efficiency	28
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	27
Senescent cells in tissue engineering	27
Editorial Board	27
In situ characterization of the tumor microenvironment	27
Mycotoxin risks are lower in biotech corn	27
Editorial overview: Tissue, cell, and pathway engineering	27
Design of DNA strand displacement reactions	26
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	26
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	26
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	26
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	26
In vitro enzyme-mediated conversion of methane and methanol toward value-added chemicals	25
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	25
Biosynthesis of advanced biofuels in microbial cell factories	25
Division of labor for substrate utilization in natural and synthetic microbial communities	25
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	25
Discovery of de novo peptides by mRNA and RaPID displays with post-translational modification methods	25
Progress and challenges in the translation of cancer nanomedicines	25
Advances in engineering substrate scope of Pseudomonas cell factories	25
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	25
Sustainability in food-waste reduction biotechnology: a critical review	24
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	24
Advances in engineering algae for biofuel production	24
Recent advances in microbial production of rare sugars and steviols	24
Advances in artificial biosynthesis of bioactive plant natural products	24
Carbon recycling with synthetic CO2 fixation pathways	24
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	24
Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	24
Recent advances in microbial synthesis of polyphenols	24
Understanding methanol metabolism through systems biology: advances and future perspectives	23
Continuous biomanufacturing with microbes — upstream progresses and challenges	23
Building mutational bridges between carbohydrate-active enzymes	23
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	23
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	23
Eubacterium limosum as a biocatalyst for sustainable methanol bioconversion	23
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	23
Drugging the microbiome and bacterial live biotherapeutic consortium production	23
Engineering microbial consortia with rationally designed cellular interactions	23
Reprogramming immunity: TAL effector-informed genome editing in rice and other crops	23

Engineering bacteria for cancer immunotherapy	23
Computational models in systems biology: standards, dissemination, and best practices	23
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	23
Quantifying nanoparticle delivery: challenges, tools, and advances	23
Shaping Aspergillus niger into a next-generation cell factory for L-malic acid	23
Approaches for a more microbiologically and chemically safe dried fruit supply chain	22
Biomolecular feedback controllers: from theory to applications	22
Engineering organoids as cerebral disease models	22
Designed dietary fibers: engineering carbohydrate structure for precision modulation of the human gut microbiome	22
Deep learning and generative artificial intelligence methods in enzyme and cell engineering	22
Cell-free enzyme cascades — application and transition from development to industrial implementation	22
Anaerobic microbial core for municipal wastewater treatment — the sustainable platform for resource recovery	21
Window of sustainable bioprocess operation: towards merging environmental sustainability assessment and process operation at early-stage bioprocess development	21
Editorial overview: Phosphate Special Issue	21
Optogenetics in medicine: innovations and therapeutic applications	21
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	21
Genetically pliable green algae for bioproduction of modified fatty acids, nutritional therapeutic oils, and biopharmaceuticals	21
Molecular biotechnology for the biodegradation of organofluorine compounds	21
Cell-free biosensors: where have we been and where do we need to go?	21
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	21
Unlocking therapeutic impacts of the gut microbiota with computational tools	21
A guide to designing cell-surface receptors in plants	21
The double life of CRISPR–Cas13	21
Architectures of emerging biofoundry platforms for synthetic biology	21
Filamentous fungi for future functional food and feed	21
Machine learning in wastewater: opportunities and challenges — “not everything is a nail!”	21
SgRNA engineering for improved genome editing and expanded functional assays	21
Technologies for the discovery of G protein–coupled receptor–targeting biologics	21
Resolving misconceptions and constraints in growth-coupled bioproduction	21
Cutting-edge plant natural product pathway elucidation	20
Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia	20
Sharp solutions to cleave plant fibers	20
Editorial overview: 21st Century solutions for crop improvement	20
Recent developments of oleaginous yeasts toward sustainable biomanufacturing	20
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	20
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	20
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	19
Engineered microbial living matter for diagnostics, prevention, and therapy	19
Spatial omics techniques and data analysis for cancer immunotherapy applications	19
Rational design of peptide-based implants for corneal bioengineering	19
Cell-free chemoenzymatic cascades with bio-based molecules	19
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	19
Advancing fungal biodegradation of nonsteroidal anti-inflammatory drugs — challenges and future perspectives	18
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	18
Technoeconomic analysis for near-term scale-up of bioprocesses	18
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	18
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	18
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	18
Cell–nano interactions of polydopamine nanoparticles	18
Valorization of waste biomass in the circular bioeconomy: advances in pretreatment and catalytic conversion technologies in modern biorefineries	17
Dissecting postharvest chilling injury through biotechnology	17
Constructing next-generation CRISPR–Cas tools from structural blueprints	17
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	17
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	17
Artificial intelligence: a solution to involution of design–build–test–learn cycle	17
Cell-free protein synthesis systems for vaccine design and production	17
Controlled protein synthesis and spatial organisation in microfluidic environments	17
Editorial Board	17
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	16
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	16
Advances in mass spectrometry-enabled multiomics at single-cell resolution	16
The dawn of interoperating spatial models in cell biology	16
Coupling and uncoupling growth and product formation for producing chemicals	16
Biotechnological interventions in reducing losses of tropical fruits and vegetables	16
Harnessing the omics revolution to address the global biodiversity crisis	16
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	16
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design	16
AuxSynBio: synthetic biology tools to understand and engineer auxin	16
The tier system: a host development framework for bioengineering	15
Mannosylerythritol lipids: production, downstream processing, and potential applications	15
Disturbance and stability dynamics in microbial communities for environmental biotechnology applications	15
Biosensor-enabled pathway optimization in metabolic engineering	15
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	15
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	15
Emerging strategies to enhance microbial natural product–based drug discovery	15
Editorial Board	15
Advances in genome-scale metabolic models of industrially important fungi	15
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	15
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	15
Large language model agents as experimental orchestrators in data-driven enzyme engineering	15
Transforming stressed plants into healthy foods	15
Editorial Board	14
Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy	14
Design and fabrication of field-deployable microbial biosensing devices	14
Corrigendum to “Advances in engineering substrate scope of Pseudomonas cell factories” [Curr Opin Biotechnol 92 (2025) 103270]	14
Anti-inflammatory and immunomodulatory properties of polysaccharides in mushrooms	14
Advancing plant science through precision 3D bioprinting: new tools for research and biotech applications	14
Using systems metabolic engineering strategies for high-oil maize breeding	14
Personalized gut microbial community modeling by leveraging genome-scale metabolic models and metagenomics	14
Tools for automated genome editing of stem cells	13
Effects of sewage sludge ash as a recycled phosphorus source on the soil microbiome	13
Harnessing the potential of the microbial sulfur cycle for environmental biotechnology	13
Corrigendum to: “Advances in consolidated bioprocessing using synthetic cellulosomes” [Curr Opin Biotechnol 2022, 78:102840]	13
Detecting antibiotic resistance genes in anthropogenically impacted streams and rivers	13
Green horizons: how plant synthetic biology can enable space exploration and drive on Earth sustainability	13