Current Opinion in Biotechnology

Article	Citations
The potential of remote sensing and artificial intelligence as tools to improve the resilience of agriculture production systems	219
Enhancing phytoremediation of soils polluted with heavy metals	172
Principles for designing an optimal mRNA lipid nanoparticle vaccine	136
Large-scale production of enzymes for biotechnology uses	97
Enhancing phage therapy through synthetic biology and genome engineering	94
The future of phage biocontrol in integrated plant protection for sustainable crop production	90
Metagenomic tools in microbial ecology research	90
Unlocking the next generation of phage therapy: the key is in the receptors	88
Overview of dietary assessment methods for measuring intakes of foods, beverages, and dietary supplements in research studies	83
Marine collagen scaffolds in tissue engineering	82
Microbial production of polyunsaturated fatty acids — high-value ingredients for aquafeed, superfoods, and pharmaceuticals	77
Technoeconomic analysis for biofuels and bioproducts	76
The role of single cell protein in cellular agriculture	75
Microalgae based production of single-cell protein	75
Advances in microphysiological blood-brain barrier (BBB) models towards drug delivery	74
Formate dehydrogenases for CO2 utilization	73
Glutathione and its precursors in cancer	71
New-to-nature chemistry from old protein machinery: carbene and nitrene transferases	69
Single-cell metabolomics: where are we and where are we going?	68
Implantable brain machine interfaces: first-in-human studies, technology challenges and trends	68
The plant microbiota: composition, functions, and engineering	66
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	66
Chain elongators, friends, and foes	65
Recent advances in understanding the ecophysiology of enhanced biological phosphorus removal	65
Synthetic co-cultures: novel avenues for bio-based processes	65

Circadian rhythms and meal timing: impact on energy balance and body weight	64
Transcription factor-based biosensors: a molecular-guided approach for natural product engineering	63
Unraveling the mechanobiology of immune cells	62
Fermented food products in the era of globalization: tradition meets biotechnology innovations	60
Vascularization in skin wound healing: where do we stand and where do we go?	59
Bacteriophage endolysins — extending their application to tissues and the bloodstream	58
Carbonic anhydrase for CO2 capture, conversion and utilization	58
Contemporary aquaculture: implications for human nutrition	57
Machine learning to navigate fitness landscapes for protein engineering	56
Reprogramming bacteriophage host range: design principles and strategies for engineering receptor binding proteins	56
Conquering CRISPR: how phages overcome bacterial adaptive immunity	55
Application of nanotechnology in medical diagnosis and imaging	54
Toward sustainable, cell-free biomanufacturing	52
Enzyme evolution for industrial biocatalytic cascades	51
The limitless applications of single-cell metabolomics	51
Recent advances in heterologous expression of natural product biosynthetic gene clusters in Streptomyces hosts	50
RNAs — a new frontier in crop protection	49
Engineering of new-to-nature ribosomally synthesized and post-translationally modified peptide natural products	49
Transporter engineering in microbial cell factories: the ins, the outs, and the in-betweens	48
Lysins breaking down the walls of Gram-negative bacteria, no longer a no-go	48
Engineering nanoparticulate vaccines for enhancing antigen cross-presentation	48
Targeting biofilms using phages and their enzymes	46
Requirements for fungal uptake of dsRNA and gene silencing in RNAi-based crop protection strategies	45
Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control	44
Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces	44
Microbes trading electricity in consortia of environmental and biotechnological significance	44
Status and challenges of electrical stimulation use in chronic wound healing	43
Engineering xylose metabolism in yeasts to produce biofuels and chemicals	42
Challenges and opportunities in biological funneling of heterogeneous and toxic substrates beyond lignin	42
Industrial production of microbial protein products	42
Antifungal natural products	42
Phages to shape the gut microbiota?	41
Stop helping pathogens: engineering plant susceptibility genes for durable resistance	41
Complexity of macrophage metabolism in infection	41
Chitosan and other edible coatings to extend shelf life, manage postharvest decay, and reduce loss and waste of fresh fruits and vegetables	41
Towards superior plant-based foods using metabolomics	41
Accelerating strain engineering in biofuel research via build and test automation of synthetic biology	40
Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production	40
Discovery of new enzymatic functions and metabolic pathways using genomic enzymology web tools	40
Building kinetic models for metabolic engineering	39
Preclinical data and safety assessment of phage therapy in humans	39
Recent progress and advanced technology in carbohydrate-based drug development	39
Advances in electrochemical cofactor regeneration: enzymatic and non-enzymatic approaches	38
Filamentous fungi for future functional food and feed	37
Enzymes in biotechnology: Critical platform technologies for bioprocess development	37
TCA cycle signalling and the evolution of eukaryotes	36
Dissecting postharvest chilling injury through biotechnology	35
Time to integrate biotechnological approaches into fish gut microbiome research	35
Phage engineering and the evolutionary arms race	35
Strategies for genotype-flexible plant transformation	35

The role of cuticle in fruit shelf-life	35
Omics-based strategies to discover novel classes of RiPP natural products	34
Biosensor-enabled pathway optimization in metabolic engineering	34
Recent advances in improving metabolic robustness of microbial cell factories	34
Recent advances in bivalve-microbiota interactions for disease prevention in aquaculture	34
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	33
Refactoring biosynthetic gene clusters for heterologous production of microbial natural products	33
Metabolic interactions in beneficial microbe recruitment by plants	33
The human gut microbiota during the initial stages of life: insights from bifidobacteria	33
New chemistries and enzymes for synthetic genetics	32
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	32
The sum is greater than the parts: exploiting microbial communities to achieve complex functions	32
Phage-specific antibodies	31
Engineering long-circulating nanomaterial delivery systems	30
Diversity: current and prospective secondary metabolites for nutrition and medicine	30
Aspergillus terreus as an industrial filamentous fungus for pharmaceutical biotechnology	30
Synthetic metabolism for biohalogenation	30
Cyanobacteria as cell factories: the roles of host and pathway engineering and translational research	30
Enzymatic browning and polyphenol oxidase control strategies	30
Resolving complex phenotypes with Raman spectroscopy and chemometrics	30
Metabolic engineering for production of functional polysaccharides	29
Combining protein and metabolic engineering to construct efficient microbial cell factories	29
Utilizing cross-species co-cultures for discovery of novel natural products	29
CO2-converting enzymes for sustainable biotechnology: from mechanisms to application	29
Calm and Frenzy: marine obligate hydrocarbonoclastic bacteria sustain ocean wellness	29
Beyond antibacterials – exploring bacteriophages as antivirulence agents	29
Siderophore natural products as pharmaceutical agents	29
Native LC–MS for capturing quality attributes of biopharmaceuticals on the intact protein level	29
Polyketide pesticides from actinomycetes	28
CRISPR-based metabolic engineering in non-model microorganisms	28
Assessing the potential to harness the microbiome through plant genetics	28
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	28
New synthetic biology tools for metabolic control	28
Perspectives for self-driving labs in synthetic biology	28
Division of labor for substrate utilization in natural and synthetic microbial communities	28
Recent progress in the synthesis of advanced biofuel and bioproducts	28
Understanding the impact of age-related changes in the gut microbiome on chronic diseases and the prospect of elderly-specific dietary interventions	28
Molecular characterization of HEK293 cells as emerging versatile cell factories	28
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	28
Gene delivery for immunoengineering	28
Neural interface systems with on-device computing: machine learning and neuromorphic architectures	28
Receptor kinases in plant responses to herbivory	27
Unlocking nature’s biosynthetic potential by directed genome evolution	27
Can recombinant milk proteins replace those produced by animals?	27
Delivery of CRISPR-Cas systems using phage-based vectors	27
Engineering microbial cell morphology and membrane homeostasis toward industrial applications	27
SpaceHort: redesigning plants to support space exploration and on-earth sustainability	27
Chassis engineering for microbial production of chemicals: from natural microbes to synthetic organisms	27
The double life of CRISPR–Cas13	26
Engineering microbial consortia with rationally designed cellular interactions	26
Enhancing enzymatic performance with nanoparticle immobilization: improved analytical and control capability for synthetic biochemistry	26
Biosynthesis of human milk oligosaccharides via metabolic engineering approaches: current advances and challenges	26
Virus-induced metabolic reprogramming and innate sensing hereof by the infected host	26
Modeling of interspecies electron transfer in anaerobic microbial communities	26
Barriers to microalgal mass cultivation	26
Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development	26
T cell metabolism in homeostasis and cancer immunity	26
Biotechnology for secure biocontainment designs in an emerging bioeconomy	26
Engineering biology approaches for food and nutrient production by cyanobacteria	25
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	25
A roadmap to engineering antiviral natural products synthesis in microbes	25
Design and engineering of artificial microbial consortia for biohydrogen production	25
Synthetic mRNA – emerging new class of drug for tissue regeneration	25
Synthetic molecular evolution of antimicrobial peptides	25
Compartmentalized biosynthesis of fungal natural products	24
Engineering of citrus to obtain huanglongbing resistance	24
Manufactured extracellular vesicles as human therapeutics: challenges, advances, and opportunities	24
Synthetic or natural? Metabolic engineering for assimilation and valorization of methanol	24
Virus-like particles for drug delivery: a review of methods and applications	24
Engineering the plant microbiota in the context of the theory of ecological communities	24
Application of bacteriophage-derived endolysins to combat streptococcal disease: current state and perspectives	24
Investigating dairy microbiome: an opportunity to ensure quality, safety and typicity	23
Microalgal lipids: biochemistry and biotechnology	23
Flexible and biodegradable electronic implants for diagnosis and treatment of brain diseases	23
Emerging concepts in bone repair and the premise of soft materials	23
Advances in quantifying circulatory microRNA for early disease detection	23
Artificial intelligence: a solution to involution of design–build–test–learn cycle	23
Recycling carbon for sustainable protein production using gas fermentation	23
Calling for a systems approach in microbiome research and innovation	23
Phage Biobank: Present Challenges and Future Perspectives	23

Bio-integrative design of the neural tissue-device interface	23
Virus-like particles against infectious disease and cancer: guidance for the nano-architect	23
Zebrafish: a big fish in the study of the gut microbiota	23
Production of recombinant and therapeutic proteins in microalgae	23
Probiotics for coral aquaculture: challenges and considerations	22
Metabolic engineering strategies to overcome precursor limitations in isoprenoid biosynthesis	22
Engineered biomaterials for heart disease	22
Different avenues for progress apply to drought tolerance, water use efficiency and yield in dry areas	22
Innovative approaches for the processes involved in microbial biodeterioration of cultural heritage materials	22
The long road to engineering durable disease resistance in wheat	22
Engineering microbial diagnostics and therapeutics with smart control	22
Strategies for engineering improved nitrogen use efficiency in crop plants via redistribution and recycling of organic nitrogen	22
Starch and sugars as determinants of postharvest shelf life and quality: some new and surprising roles	22
Successful delivery of nutrition programs and the sustainable development goals	22
Transient electronics: new opportunities for implantable neurotechnology	22
Yeast based biorefineries for oleochemical production	22
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	21
Ecological and molecular perspectives on responders and non-responders to probiotics and prebiotics	21
Cytochromes P450 in the biocatalytic valorization of lignin	21
Large-scale genome editing in plants: approaches, applications, and future perspectives	21
Phage-based target discovery and its exploitation towards novel antibacterial molecules	21
C2 feedstock-based biomanufacturing of value-added chemicals	21
Integration of omics data to unravel root microbiome recruitment	21
Biotechnological approaches for controlling postharvest fruit softening	21
Mediated electrochemistry for redox-based biological targeting: entangling sensing and actuation for maximizing information transfer	21
Mining and design of biosensors for engineering microbial cell factory	21
Phages in vaccine design and immunity; mechanisms and mysteries	21
Heterologous expression and metabolic engineering tools for improving terpenoids production	21
Genome mining strategies for metallophore discovery	20
Engineering immunogenic cell death with nanosized drug delivery systems improving cancer immunotherapy	20
Enzymatic upgrading of heteroxylans for added-value chemicals and polymers	20
Continuous biomanufacturing with microbes — upstream progresses and challenges	20
Beyond calorie restriction: aging as a biological target for nutrient therapies	20
Engineering spinal cord repair	20
Looking into the future of phage-based control of zoonotic pathogens in food and animal production	20
Stability, robustness, and containment: preparing synthetic biology for real-world deployment	19
Monoclonal antibody therapies for COVID-19: lessons learned and implications for the development of future products	19
Illuminating the cross-talk between tumor metabolism and immunity in IDH-mutated cancers	19
Genome editing systems across yeast species	19
Microbial production of chemicals driven by CRISPR-Cas systems	19
Advancing mechanistic understanding and bioengineering of probiotic lactobacilli and bifidobacteria by genome editing	19
Engineering insect resistance using plant specialized metabolites	19
Cyanobacteria as whole-cell factories: current status and future prospectives	19
Recent advances in synthetic glycoengineering for biological applications	19
Biotechnological application of Streptomyces for the production of clinical drugs and other bioactive molecules	19
Taking the pulse of bioprocesses: at-line and in-line monitoring of mammalian cell cultures	18
Heterologous biosynthesis as a platform for producing new generation natural products	18
Using genome and transcriptome analysis to elucidate biosynthetic pathways	18
Analytical methods to characterize recombinant adeno-associated virus vectors and the benefit of standardization and reference materials	18
Biosystem design of Corynebacterium glutamicum for bioproduction	18
Carbohydrate-Active enZyme (CAZyme) enabled glycoengineering for a sweeter future	18
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	18
Engineering phototrophic bacteria for the production of terpenoids	18
Era of gapless plant genomes: innovations in sequencing and mapping technologies revolutionize genomics and breeding	18
Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein and hairy roots: a perfect match for gene functional analysis and crop improvement	18
Potential use of microbial engineering in single-cell protein production	17
Mechanogenetics: harnessing mechanobiology for cellular engineering	17
Modeling the energy metabolism in immune cells	17
Cell-free technologies for biopharmaceutical research and production	17
Using plant chemistry to improve interactions between plants, herbivores and their natural enemies: challenges and opportunities	17
Crops for dry environments	17
Harnessing in vitro platforms for natural product research: in vitro driven rational engineering and mining (iDREAM)	17
Mannosylerythritol lipids: production, downstream processing, and potential applications	17
Machine learning for predicting phenotype from genotype and environment	17
Targeting ripening regulators to develop fruit with high quality and extended shelf life	17
Computational tools and resources for designing new pathways to small molecules	17
Genetic manipulation of phages for therapy using BRED	17
Practical monitoring technologies for cells and substrates in biomanufacturing	17
The emerging role of one-carbon metabolism in T cells	16
Protein engineering for electrochemical biosensors	16
Synthetic apomixis: the beginning of a new era	16
Anaerobic fluorescent reporters for cell identification, microbial cell biology and high-throughput screening of microbiota and genomic libraries	16
Multifunctional cellulases are potent, versatile tools for a renewable bioeconomy	16
Harnessing the omics revolution to address the global biodiversity crisis	16
Engineering bionanoparticles for improved biosensing and bioimaging	16
Cell-free enzyme cascades — application and transition from development to industrial implementation	16
Control strategies to manage trade-offs during microbial production	16
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	16
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	16
Supercapacitive biofuel cells	16
Recent applications of biotechnological approaches to elucidate the biology of plant–nematode interactions	15
Enzymology and biosynthesis of the orsellinic acid derived medicinal meroterpenoids	15
Biomolecular feedback controllers: from theory to applications	15
Enzymatic and non-enzymatic functional attributes of plant microbiome	15
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	15
Metabolic and cellular engineering for the production of natural products	15
Recent advances in biotechnology for marine enzymes and molecules	15
Partners for life: building microbial consortia for the future	15
Directing carbohydrates toward ethanol using mesophilic microbial communities	15
Engineering healthy crops: molecular strategies for enhancing the plant immune system	15
Leveraging millets for developing climate resilient agriculture	15
New opportunities for genetic code expansion in synthetic yeast	15
Developmental biology-inspired tissue engineering by combining organoids and 3D bioprinting	15
Computational design of vaccine immunogens	15
Optogenetic interrogation and control of cell signaling	15