Current Opinion in Biotechnology

Article	Citations
Editorial Board	311
Editorial Board	254
Corrigendum to “Methylotrophs: from C1 compounds to food” [Curr Opin Biotechnol 75 (2022) 102685]	206
Stem cells characterization: OMICS reinforcing analytics	195
Anaerobic microbial methanol utilization as a one-carbon feedstock	116
Creating value from purple phototrophic bacteria via single-cell protein production	115
Cell-free technologies for biopharmaceutical research and production	105
Systems biology-based analysis of cell-free systems	103
Tumor lipid metabolism: a mechanistic link between diet and cancer progression	99
Bioelectrosynthesis systems	97
The osteo-angiogenic signaling crosstalk for bone regeneration: harmony out of complexity	95
How much could improving photosynthesis increase crop yields? A call for systems-level perspectives to guide engineering strategies	93
The journey of a lifetime — development of Pfizer’s COVID-19 vaccine	92
Recent progress in the synthesis of advanced biofuel and bioproducts	90
Circadian rhythms and meal timing: impact on energy balance and body weight	89
Applications of artificial intelligence to enzyme and pathway design for metabolic engineering	89
Synthetic biology advances towards a bio-based society in the era of artificial intelligence	85
Editorial Board	85
Leveraging rAAV bioprocess understanding and next generation bioanalytics development	82
Engineered T cells and macrophages: two arms to seize solid tumors	81
A molecular assessment of the practical potential of DNA-based computation	80
Recent advances, opportunities and challenges in cybergenetic identification and control of biomolecular networks	72
Engineered living bioassemblies for biomedical and functional material applications	70
Bridging the gap — biomimetic design of bioelectronic interfaces	65
Single-cell analysis opens a goldmine for plant functional studies	63

Coupling chemistry and biology for the synthesis of advanced bioproducts	63
Emerging technologies for genetic control systems in cellular therapies	62
Whole-plant phenotypic engineering: moving beyond ratios for multi-objective optimization of nutrient use efficiency	61
Engineering the plant microbiota in the context of the theory of ecological communities	61
Lighting the way: recent developments and applications in molecular optogenetics	61
Fermented food products in the era of globalization: tradition meets biotechnology innovations	59
The plant microbiota: composition, functions, and engineering	54
Innovations and trends in antibody repertoire analysis	52
Deep learning in regulatory genomics: from identification to design	52
Genetically encoded elastin-like polypeptide nanoparticles for drug delivery	52
Emerging trends in production of plant natural products and new-to-nature biopharmaceuticals in yeast	51
Biocatalytic cascade transformations for the synthesis of C-nucleosides and N-nucleoside analogs	50
Multifaceted metabolic role of infections in the tumor microenvironment	50
Using genome and transcriptome analysis to elucidate biosynthetic pathways	50
2-hydroxyglutarate rides the cancer-immunity cycle	49
Regulatory roles of microRNAs in insect pests: prospective targets for insect pest control	47
Engineering of the genetic code	47
Computational tools and resources for designing new pathways to small molecules	46
Dietary microalgae on poultry meat and eggs: explained versus unexplained effects	46
Contemporary aquaculture: implications for human nutrition	45
Perspectives for self-driving labs in synthetic biology	45
Machine learning to navigate fitness landscapes for protein engineering	44
Mass spectrometry imaging methods for visualizing tumor heterogeneity	44
Editorial overview: Biotechnological insights of marine and terrestrial microbiome	43
Computational approaches to understanding nutrient metabolism and metabolic disorders	42
Editorial Board	42
Editorial Board	42
Anaerobic fluorescent reporters for cell identification, microbial cell biology and high-throughput screening of microbiota and genomic libraries	41
Harnessing the potential of microbial methane utilization for chasing sustainability	41
Toolboxes for plant systems biology research	41
Tissue engineering in the agri-food industry: current status, socio-economic overview and regulatory compliance	41
Advancing in vivo reprogramming with synthetic biology	40
Matching diverse feedstocks to conversion processes for the future bioeconomy	40
Plant synthetic biology as a tool to help eliminate hidden hunger	40
Reducing crop losses by gene-editing control of organ developmental physiology	40
Analytics of host cell proteins (HCPs): lessons from biopharmaceutical mAb analysis for Gene therapy products	39
The potential of amoeba-based processes for natural product syntheses	39
Integration of omics data to unravel root microbiome recruitment	39
Redox as a bioprocess parameter: analytical redox quantification in biological therapeutic production	38
Metabolic and cellular engineering for the production of natural products	38
Understanding atmospheric intercontinental dispersal of harmful microorganisms	38
Editorial overview: Emerging routes to sustainable energy capture and conversion into value-added products	38
Sourcing DNA parts for synthetic biology applications in plants	38
Corrigendum to: “Design, construction, and application of noncanonical redox cofactor infrastructures” [Curr Opin Biotechnol 84 (2023) 103019]	38
Impact of anthropogenic contamination on glacier surface biota	38
Single-cell omics analysis with genome-scale metabolic modeling	38
Pathway engineering for the biosynthesis of psychedelics	36
Synthetic biology approaches for dynamic CHO cell engineering	36
Low-cost, point-of-care biomarker quantification	36
Editorial overview: Pioneering innovations in food biotechnology	36

Synthetic apomixis: the beginning of a new era	36
Overview of dietary assessment methods for measuring intakes of foods, beverages, and dietary supplements in research studies	35
The aging tumor metabolic microenvironment	35
Next-generation biological vector platforms for in vivo delivery of genome editing agents	35
Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes	35
Biochemical production with microbial bioelectrochemical systems	35
Complexity of subcellular metabolism: strategies for compartment-specific profiling	35
Synthetic mRNA – emerging new class of drug for tissue regeneration	35
Synthetic fuels: what are they and where do they come from?	35
Editorial overview: Nanobiotechnology for immunoengineering	35
Editorial Board	35
Extracellular vesicles as therapeutics for inflammation and infection	35
Antigen-specific nanomedicines for the treatment of autoimmune disease: target cell types, mechanisms and outcomes	34
Matchmaking at the cell surface using bispecifics to put cells on their best behavior	34
Editorial Board	34
Strategies and opportunities for engineering antifungal peptides for therapeutic applications	34
Potential use of microbial engineering in single-cell protein production	34
Engineered methane biocatalysis: strategies to assimilate methane for chemical production	33
Targeting ripening regulators to develop fruit with high quality and extended shelf life	33
Programmable synthetic biology tools for developing microbial cell factories	33
Understanding and harnessing the complexity of interspecies interactions in acetogenic mixotrophic co-cultures	33
What’s new in single-cell proteomics	33
Ketogenic regimens for acute neurotraumatic events	33
Engineering insect resistance using plant specialized metabolites	32
Editorial Board	32
Editorial overview: Biotechnology to help understand and harness biotic interactions in plants	32
Strategies for uncovering stable isotope tracing patterns between cell populations	32
Optimizing the value of bioinks and robotics to advance in vivo bioprinting	31
Challenges and future perspectives for high-throughput chimeric antigen receptor T cell discovery	31
Practical monitoring technologies for cells and substrates in biomanufacturing	31
Bridging the gender gap in autoimmunity with T-cell–targeted biomaterials	31
Engineering bacterial warriors: harnessing microbes to modulate animal physiology	31
Biohybrid approaches to interface with the nervous system: the best of both worlds	31
Beyond vancomycin: recent advances in the modification, reengineering, production and discovery of improved glycopeptide antibiotics to tackle multidrug-resistant bacteria	30
Can digital twin efforts shape microorganism-based alternative food?	30
De novo domestication: retrace the history of agriculture to design future crops	30
Inhalable mRNA vaccines for respiratory diseases: a roadmap	30
Avoidance of detrimental defense responses in beneficial plant–microbe interactions	30
Editorial Board	29
Machine learning for predicting phenotype from genotype and environment	29
Editorial overview: Tissue, cell, and pathway engineering	29
Microbiological food safety considerations in shelf-life extension of fresh fruits and vegetables	29
Life stage-dependent genetic traits as drivers of plant–herbivore interactions	29
Analytical tools for unravelling the metabolism of gas-fermenting Clostridia	29
Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria	29
Sustainability in food-waste reduction biotechnology: a critical review	28
In situ characterization of the tumor microenvironment	28
Quantitative and analytical tools to analyze the spatiotemporal population dynamics of microbial consortia	28
Senescent cells in tissue engineering	28
Biologics-based degraders — an expanding toolkit for targeted-protein degradation	28
An integrated eco-evolutionary framework to predict population-level responses of climate-sensitive pathogens	28
Two in one: the emerging concept of bifunctional antibodies	27
Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks	27
Engineering plant–microbe communication for plant nutrient use efficiency	27
Division of labor for substrate utilization in natural and synthetic microbial communities	27
Corrigendum to “Unraveling the mechanobiology of immune cells” [Curr Opin Biotechnol 66 (2020) 236-245]	27
Recent trends in the modification of polyphenolic compounds using hydroxylation and glycosylation	27
Mycotoxin risks are lower in biotech corn	27
Engineering next-generation chimeric antigen receptor-T cells: recent breakthroughs and remaining challenges in design and screening of novel chimeric antigen receptor variants	27
Achieving net zero CO2 emission in the biobased production of reduced platform chemicals using defined co-feeding of methanol	27
Advances in engineering substrate scope of Pseudomonas cell factories	27
Genetic and process engineering for polyhydroxyalkanoate production from pre- and post-consumer food waste	26
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection	26
Clustered regularly interspaced short palindromic repeats tools for plant metabolic engineering: achievements and perspectives	26
Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules	26
General framework for enzyme-photo-coupled catalytic system toward carbon dioxide conversion	26
Decoding dissolved information: environmental DNA sequencing at global scale to monitor a changing ocean	26
Recent advances in microbial synthesis of polyphenols	25
Force in numbers: high-throughput screening approaches to unlock microbial transport	25
Integrating bioprinting and optogenetic technologies for precision plant tissue engineering	25
Computational models in systems biology: standards, dissemination, and best practices	25
Recent advances in microbial production of rare sugars and steviols	25
Advances in engineering algae for biofuel production	25
Progress and challenges in the translation of cancer nanomedicines	25
Proteomics insights into the fungal-mediated bioremediation of environmental contaminants	24
Path to zero emission of nitrous oxide in sewage treatment: is nitrification controllable or avoidable?	24
Chemical speciation of trace metals in mammalian cell culture media: looking under the hood to boost cellular performance and product quality	24
Engineering bacteria for cancer immunotherapy	24
Carbon recycling with synthetic CO2 fixation pathways	24
Hitting the sweet spot with capillary electrophoresis: advances in N-glycomics and glycoproteomics	24

Cytochromes P450 in the biocatalytic valorization of lignin	23
Getting better all the time — recent progress in the development of CRISPR/Cas-based tools for plant genome engineering	23
RNAs — a new frontier in crop protection	23
Continuous biomanufacturing with microbes — upstream progresses and challenges	23
Combining enzyme and metabolic engineering for microbial supply of therapeutic phytochemicals	23
Machine learning applications to improve flavor and nutritional content of horticultural crops through breeding and genetics	23
Engineering microbial consortia with rationally designed cellular interactions	23
Pleiotropy, a feature or a bug? Toward co-ordinating plant growth, development, and environmental responses through engineering plant hormone signaling	23
Approaches for a more microbiologically and chemically safe dried fruit supply chain	23
Drugging the microbiome and bacterial live biotherapeutic consortium production	23
Cellobiose dehydrogenase in biofuel cells	22
In vivo 2H/13C flux analysis in metabolism research	22
Cell-free enzyme cascades — application and transition from development to industrial implementation	22
Anaerobic microbial core for municipal wastewater treatment — the sustainable platform for resource recovery	22
Engineering organoids as cerebral disease models	22
Editorial Board	22
Building mutational bridges between carbohydrate-active enzymes	22
Biomolecular feedback controllers: from theory to applications	22
Quantifying nanoparticle delivery: challenges, tools, and advances	22
Window of sustainable bioprocess operation: towards merging environmental sustainability assessment and process operation at early-stage bioprocess development	21
Optogenetics in medicine: innovations and therapeutic applications	21
Recent advances in the biological depolymerization and upcycling of polyethylene terephthalate	21
Time to integrate biotechnological approaches into fish gut microbiome research	21
Editorial overview: Tissue, cell and pathway engineering: bioinspired, biomimetic and hybrid neural interfaces	21
Technologies for the discovery of G protein–coupled receptor–targeting biologics	21
Filamentous fungi for future functional food and feed	21
Editorial overview: Phosphate Special Issue	21
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology	20
Machine learning in wastewater: opportunities and challenges — “not everything is a nail!”	20
Ultrasonographic features of ovarian morphology capture nutritional and metabolic influences on the reproductive axis: implications for biomarker development in ovulatory disorders	20
Antimicrobial polymeric biomaterials based on synthetic, nanotechnology, and biotechnological approaches	20
Industrial potential of the enzymatic synthesis of nucleoside analogs: existing challenges and perspectives	20
Food industry side streams: an unexploited source for biotechnological phosphorus upcycling	20
Microbial production of chemicals driven by CRISPR-Cas systems	20
The double life of CRISPR–Cas13	20
SgRNA engineering for improved genome editing and expanded functional assays	20
Editorial overview: 21st Century solutions for crop improvement	20
Coupling electrochemical CO2 reduction to microbial product generation – identification of the gaps and opportunities	19
Advancing fungal biodegradation of nonsteroidal anti-inflammatory drugs — challenges and future perspectives	19
Cell-free chemoenzymatic cascades with bio-based molecules	19
Cell-free protein synthesis systems for vaccine design and production	19
Precise control methods of the physicochemical properties of nanoparticles for personalized medicine	19
Engineering metalloproteinase inhibitors: tissue inhibitors of metalloproteinases or antibodies, that is the question	19
Artificial intelligence: a solution to involution of design–build–test–learn cycle	19
Recent developments of oleaginous yeasts toward sustainable biomanufacturing	19
Technoeconomic analysis for near-term scale-up of bioprocesses	19
Phage biocontrol for reducing bacterial foodborne pathogens in produce and other foods	19
Cutting-edge plant natural product pathway elucidation	18
Engineering ribosomally synthesized and posttranslationally modified peptides as new antibiotics	18
Bio-integrative design of the neural tissue-device interface	18
Cell–nano interactions of polydopamine nanoparticles	18
Surveilling cellular vital signs: toward label-free biosensors and real-time viability assays for bioprocessing	18
Dissecting postharvest chilling injury through biotechnology	18
Rational design of peptide-based implants for corneal bioengineering	18
Carbon-efficient waste upcycling: combining syngas fermentation and chain elongation with synthetic consortia	18
Spatial omics techniques and data analysis for cancer immunotherapy applications	18
Toward manipulating serotonin signaling via the microbiota–gut–brain axis	18
Sharp solutions to cleave plant fibers	18
Engineered microbial living matter for diagnostics, prevention, and therapy	18
Tissue-resident macrophages — early passengers or drivers in the tumor niche?	17
Process intensification in continuous flow biocatalysis by up and downstream processing strategies	17
Editorial Board	17
Recent advances in synthetic glycoengineering for biological applications	17
Petrosia ficiformis (Poiret, 1789): an excellent model for holobiont and biotechnological studies	17
Constructing next-generation CRISPR–Cas tools from structural blueprints	17
Editorial overview: Unlocking the secrets of nongenetic plasticity, one cell at a time	17
Glycosyltransferases in human milk oligosaccharide synthesis: structural mechanisms and rational design	17
Coral holobionts and biotechnology: from Blue Economy to coral reef conservation	17
Identification of novel membrane proteins for improved lignocellulose conversion	17
Coupling and uncoupling growth and product formation for producing chemicals	17
Adaptive biomimicry: design of neural interfaces with enhanced biointegration	17
Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes	16
Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis	16
Harnessing the omics revolution to address the global biodiversity crisis	16
The tier system: a host development framework for bioengineering	16
Advances in mass spectrometry-enabled multiomics at single-cell resolution	16
The dawn of interoperating spatial models in cell biology	16
Biotechnological interventions in reducing losses of tropical fruits and vegetables	16
AuxSynBio: synthetic biology tools to understand and engineer auxin	16
N-acetyl-aspartate metabolism at the interface of cancer, immunity, and neurodegeneration	16
Advances in designed bionanomolecular assemblies for biotechnological and biomedical applications	16
Pseudomonas putida as a synthetic biology chassis and a metabolic engineering platform	16
Biological recognition and cellular trafficking of targeted RNA-lipid nanoparticles	16
Transdermal sensing: in-situ non-invasive techniques for monitoring of human biochemical status	16
Harnessing Fe(II)/α-ketoglutarate-dependent oxygenases for structural diversification of fungal meroterpenoids	16
Thermodynamic tools for more efficient biotechnological processes: an example in poly-(3-hydroxybutyrate) production from carbon monoxide	16
Corrigendum to “Advances in engineering substrate scope of Pseudomonas cell factories” [Curr Opin Biotechnol 92 (2025) 103270]	15
Biotechnological polyphosphate as an opportunity to contribute to the circularization of the phosphate economy	15
CRISPR-based metabolic engineering in non-model microorganisms	15
Transforming stressed plants into healthy foods	15
Personalized gut microbial community modeling by leveraging genome-scale metabolic models and metagenomics	15
Machine learning for synthetic gene circuit engineering	15
Advancing plant science through precision 3D bioprinting: new tools for research and biotech applications	15
Advances in vascularization and innervation of constructs for neural tissue engineering	15
Diversity: current and prospective secondary metabolites for nutrition and medicine	15