Biotechniques

Papers
(The TQCC of Biotechniques is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-11-01 to 2025-11-01.)
ArticleCitations
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations84
UK Biobank: what can it do, how you can use it and how is it being used?84
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones60
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure58
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development47
Correction34
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots22
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment21
Rectal swab DNA collection protocol for PCR genotyping in rats19
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations18
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system17
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue15
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study15
Education in Microscopy: Taking a Closer Look with Jennifer Waters14
Welcome to the 72nd Volume of BioTechniques14
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations13
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach13
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation13
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR12
How has CRISPR Transformed Therapeutic Drug Discovery?11
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide11
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography9
Gel Purification of gDNA for Next-Generation Sequencing Applications9
Who wants to live forever? Models shaping the future of aging research9
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells9
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage8
The Forensic Genomics Toolbox is Expanding8
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds8
Selective Expansion of Target Cells Using the Enrich TROVO Platform8
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )7
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples7
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data7
Techniques to Save the Sea7
When is an SNP not an SNP?7
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection6
Corrigendum6
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent6
Correction6
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR6
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology6
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics6
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?5
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues5
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography5
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.5
X Marks the Spot: Mapping the Brain with EEG and MRI5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves5
Smart PCR Machines Can Reduce the Risk of Carryover Contamination5
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA5
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels5
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis4
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction4
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field4
Extraterrestrial agriculture: plant cultivation in space4
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern4
Adenovirus Vector System: Construction, History and Therapeutic Applications4
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies3
A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase3
Microbial Forensics: What We've Learned from Amerithrax and Beyond3
Sampling and analysis methods of air-borne microorganisms in hospital air: a review3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection3
A Simplified Procedure for Isolation of Primary Murine Microglia3
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis3
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization3
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital3
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates3
Decoding the Mechanisms of Embryo Development with Spatial Biology3
Development of an enhanced anti-pan-N-formylmethionine-specific antibody3
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information3
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis3
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)3
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli3
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle3
A drug screening platform for protein expression levels in neurological disorders3
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay3
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes3
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics3
Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications3
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE3
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems3
Premature Termination Codon: A Tunable Protein Translation Approach3
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