OOIR: Observatory of International Research

Papers

(The TQCC of Biotechniques is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)

Article	Citations
Recombinant Bacterial Endotoxin Testing: A Proven Solution	62
UK Biobank: what can it do, how you can use it and how is it being used?	55
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	46
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	40
Ancient DNA Analysis from Epoxy Resin Biodur ^® -embedded bones	40
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	24
Rectal swab DNA collection protocol for PCR genotyping in rats	23
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	19
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	19
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	18
Correction	16
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	14
Welcome to the 72nd Volume of BioTechniques	13
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	13
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	12
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	12
Comparison of DNA Extraction Methods for Samples from Old Blood Collections	11
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	11
Education in Microscopy: Taking a Closer Look with Jennifer Waters	10
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	10
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	10
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	10
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	9
The Forensic Genomics Toolbox is Expanding	9
Selective Expansion of Target Cells Using the Enrich TROVO Platform	9

Secure and Optimized Detection of PNPLA3 rs738409 Genotype by an Improved PCR–Restriction Fragment Length Polymorphism Method	9
Gel Purification of gDNA for Next-Generation Sequencing Applications	9
How has CRISPR Transformed Therapeutic Drug Discovery?	9
Who wants to live forever? Models shaping the future of aging research	7
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	7
Techniques to Save the Sea	7
Tergitol-15-S-9 Inactivates SARS-CoV-2 and Boosts Immunoassay Signals	7
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	7
Improved SARS-CoV-2 PCR Detection and Genotyping with Double-Bubble Primers	7
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	7
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	7
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	6
When is an SNP not an SNP?	6
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	6
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	6
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	6
Corrigendum	6
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	6
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	6
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	5
Design and Characterization of a 3D-printed Staggered Herringbone Mixer	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	5
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	5
Extraterrestrial agriculture: plant cultivation in space	5
Urgency of Rapid Legionella Detection Post-COVID-19 Lockdowns: an Interview with Elizabeth Minogue	5
A Simplified Method for Bronchoalveolar Lavage in Mice by Orotracheal Intubation Avoiding Tracheotomy	5
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	4
Adenovirus Vector System: Construction, History and Therapeutic Applications	4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	4
X Marks the Spot: Mapping the Brain with EEG and MRI	4
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	4
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	4
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	4
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	4
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	4
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	4
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Who Owns Your DNA?	4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	4
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	4
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	4