OOIR: Observatory of International Research

Papers

(The TQCC of Biotechniques is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-02-01 to 2025-02-01.)

Article	Citations
UK Biobank: what can it do, how you can use it and how is it being used?	41
A Clinically Validated Human Saliva Metatranscriptomic Test for Global Systems Biology Studies	36
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	35
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	30
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	26
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	24
Unraveling Crime Scenes Strand by Strand: The Forensic Odyssey of Bruce Budowle	24
Recombinant Bacterial Endotoxin Testing: A Proven Solution	21
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	19
A Novel Click Chemistry-based Peptide ELISA Protocol: Development and Technical Evaluation	16
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	15
Extraterrestrial agriculture: plant cultivation in space	14
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	12
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	11
Isothermal Amplification Using Sequence-Specific Fluorescence Detection of SARS Coronavirus 2 and Variants in Nasal Swabs	11
Effects of a Fully Enclosed Hollow-Fiber Centrifugal Ultrafiltration Technique for Laboratory Biosafety Improvement	10
Ancient DNA Analysis from Epoxy Resin Biodur ^® -embedded bones	10
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	10
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	10
X Marks the Spot: Mapping the Brain with EEG and MRI	9
Toxoplasma Gondii : The Kitty Litter Parasite	9
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	9
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	9
Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	8
Cloning Vectors for Gene Delivery, Integration and Expression in Campylobacter Jejuni	8

Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	7
Adaptation of the HEp-2 Cell Line to toTally Animal-Free Culture Systems and Real-Time Analysis of Cell Growth	7
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	7
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	7
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	7
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	7
Rapid Microfluidic Isolation of Virally Infected Primary Bronchial Epithelial Cells for Single-Cell RNA Sequencing	7
A Rapid and Sensitive Method to Measure Numbers of Live Cells in Alginate Capsules Following Depolymerization with Ethylenediaminetetraacetic Acid	6
Digital Holographic Microscopy: A Noninvasive Method to Analyze the Formation of Spheroids	6
An Easy Method for Quantification of Anaerobic and Microaerobic Gene Expression with Fluorescent Reporter Proteins	6
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	6
A Practical Approach to Genome Assembly and Annotation of Basidiomycota Using the Example of Armillaria	6
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	5
A Simple Approach for Effective Shearing and Reliable Concentration Measurement of Ultra-High-Molecular-Weight DNA	5
Adipocyte ABCA1 expression analysis using flow cytometry	5
An Approach to Identifying Quality Research Antibodies	5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
Multiparameter Screen Optimizes Immunoprecipitation	5
Phylobook: a tool for display, clade annotation and extraction of sequences from molecular phylogenies	4
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	4
Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d	4
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	4
Comparison and optimization of protocols and whole-genome capture conditions for ancient DNA samples	4
Screening for Androgen Agonists using Autonomously Bioluminescent HEK293 Reporter Cells	4
A standardized protocol for sample preparation for scanning electron microscopy to visualize extrachromosomal DNA	4
Development and validation of a portable device for lab-free versatile nucleic acid extraction	4
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	4
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	4
Novel buffer for long-term preservation of DNA in biological material at room temperature	4
Rapid Visual Detection of SARS-CoV-2 by Colorimetric Loop-mediated Isothermal Amplification	4
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	4
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	4
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	4
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	4
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	4
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	4
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	4
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	4
Overcoming Problems to Produce the Recombinant Protein LipL21 of Leptospira interrogans	3
Rectal swab DNA collection protocol for PCR genotyping in rats	3
eDNA Extraction Protocol for Metagenomic Studies in Tropical Soils	3
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	3
Accelerated Aging of Forensically Relevant Biological Materials on Swabs	3
Assay System for Mesocotyl Elongation and Hydrotropism of Maize Primary Root in Response to Low Moisture Gradient	3
Correction	3
A domed window chamber for multi-modality optical imaging	3
From Bench to Bedside: Three Imperatives for Making the Digital Laboratory a Reality	3
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	3
Reliable Budding Pattern Classification of Yeast Cells with Time-Resolved Measurement of Metabolite Production	3
Targeted Proteomics Analysis of Plasma Proteins Using Recombinant Protein Standards for Addition Only Workflows	3

The Determination of Cystatin C in Biological Samples via the Surface Plasmon Resonance Method	3
CRISPR in 3D: Innovations in Disease Modelling and Personalized Medicine	3
Design Considerations for Point-of-Need Devices Based on Nucleic Acid Amplification for COVID-19 Diagnostics and Beyond	3
Mass Spectrometry: An Old Dog with New Tricks	3
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	3
Lab Techniques for a More Sustainable World	3