OOIR: Observatory of International Research

Papers

(The TQCC of Biotechniques is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-01-01 to 2025-01-01.)

Article	Citations
A Clinically Validated Human Saliva Metatranscriptomic Test for Global Systems Biology Studies	41
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	36
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	35
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	33
Unraveling Crime Scenes Strand by Strand: The Forensic Odyssey of Bruce Budowle	30
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	26
Antibody-mediated Soluble CD14 Stabilization Prevents Agitation-Induced Increases in Presepsin Levels in Blood Component Specimens	24
Recombinant Bacterial Endotoxin Testing: A Proven Solution	24
A Novel Click Chemistry-based Peptide ELISA Protocol: Development and Technical Evaluation	21
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	21
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	19
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	16
Extraterrestrial agriculture: plant cultivation in space	15
Isothermal Amplification Using Sequence-Specific Fluorescence Detection of SARS Coronavirus 2 and Variants in Nasal Swabs	14
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	14
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	12
Effects of a Fully Enclosed Hollow-Fiber Centrifugal Ultrafiltration Technique for Laboratory Biosafety Improvement	11
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	11
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	10
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	10
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	10
Ancient DNA Analysis from Epoxy Resin Biodur ^® -embedded bones	10
Toxoplasma Gondii : The Kitty Litter Parasite	9
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	9
Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	9

X Marks the Spot: Mapping the Brain with EEG and MRI	9
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	8
Cloning Vectors for Gene Delivery, Integration and Expression in Campylobacter Jejuni	8
Adaptation of the HEp-2 Cell Line to toTally Animal-Free Culture Systems and Real-Time Analysis of Cell Growth	8
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	7
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	7
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	7
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	7
Rapid Microfluidic Isolation of Virally Infected Primary Bronchial Epithelial Cells for Single-Cell RNA Sequencing	7
A Rapid and Sensitive Method to Measure Numbers of Live Cells in Alginate Capsules Following Depolymerization with Ethylenediaminetetraacetic Acid	7
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	7
Digital Holographic Microscopy: A Noninvasive Method to Analyze the Formation of Spheroids	6
A Practical Approach to Genome Assembly and Annotation of Basidiomycota Using the Example of Armillaria	6
An Easy Method for Quantification of Anaerobic and Microaerobic Gene Expression with Fluorescent Reporter Proteins	6
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	6
Adipocyte ABCA1 expression analysis using flow cytometry	6
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
An Approach to Identifying Quality Research Antibodies	5
A Simple Approach for Effective Shearing and Reliable Concentration Measurement of Ultra-High-Molecular-Weight DNA	5
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	5
Phylobook: a tool for display, clade annotation and extraction of sequences from molecular phylogenies	5
Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d	5
Multiparameter Screen Optimizes Immunoprecipitation	5
eDNA Extraction Protocol for Metagenomic Studies in Tropical Soils	4
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	4
Development and validation of a portable device for lab-free versatile nucleic acid extraction	4
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	4
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	4
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	4
A domed window chamber for multi-modality optical imaging	4
A Method for the Temperature-Controlled Extraction of DNA from Ancient Bones	4
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	4
Design Considerations for Point-of-Need Devices Based on Nucleic Acid Amplification for COVID-19 Diagnostics and Beyond	4
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	4
Mass Spectrometry: An Old Dog with New Tricks	4
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	4
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	4
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	4
Screening for Androgen Agonists using Autonomously Bioluminescent HEK293 Reporter Cells	4
Targeted Proteomics Analysis of Plasma Proteins Using Recombinant Protein Standards for Addition Only Workflows	4
Reliable Budding Pattern Classification of Yeast Cells with Time-Resolved Measurement of Metabolite Production	4
Quantifying Sequencing Error and Effective Sequencing Depth of Liquid Biopsy NGS with UMI Error Correction	4
New Cloning Vectors to Facilitate Quick Allelic Exchange in Gram-Negative Bacteria	4
A standardized protocol for sample preparation for scanning electron microscopy to visualize extrachromosomal DNA	3
From Bench to Bedside: Three Imperatives for Making the Digital Laboratory a Reality	3
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	3
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	3
Application of a Robust Microplate Assay to Determine Induced β-1,3-glucanase and Chitinase Activity in the Cotton Plant	3
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	3
Rectal swab DNA collection protocol for PCR genotyping in rats	3

Overcoming Problems to Produce the Recombinant Protein LipL21 of Leptospira interrogans	3
The Determination of Cystatin C in Biological Samples via the Surface Plasmon Resonance Method	3
Lab Techniques for a More Sustainable World	3
A Single-wavelength Flow Cytometric Approach using Redox-sensitive Green Fluorescent Protein Probes for Measuring Redox Stress in Live Cells	3
An Assay of Human Tyrosine Protein Kinase ABL Activity using an Escherichia Coli Protein Expression System	3
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	3
Assay System for Mesocotyl Elongation and Hydrotropism of Maize Primary Root in Response to Low Moisture Gradient	3
Accelerated Aging of Forensically Relevant Biological Materials on Swabs	3
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	3
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	3
Rapid Visual Detection of SARS-CoV-2 by Colorimetric Loop-mediated Isothermal Amplification	3