OOIR: Observatory of International Research

Papers

(The TQCC of Biotechniques is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-06-01 to 2025-06-01.)

Article	Citations
UK Biobank: what can it do, how you can use it and how is it being used?	64
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	60
Ancient DNA Analysis from Epoxy Resin Biodur ^® -embedded bones	48
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	46
Rectal swab DNA collection protocol for PCR genotyping in rats	41
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	26
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	25
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	20
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	18
Correction	16
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	14
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	14
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	13
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	13
Welcome to the 72nd Volume of BioTechniques	13
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	12
Education in Microscopy: Taking a Closer Look with Jennifer Waters	12
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	11
The Forensic Genomics Toolbox is Expanding	11
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	11
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	11
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	10
How has CRISPR Transformed Therapeutic Drug Discovery?	10
Who wants to live forever? Models shaping the future of aging research	9
Gel Purification of gDNA for Next-Generation Sequencing Applications	9

Improved SARS-CoV-2 PCR Detection and Genotyping with Double-Bubble Primers	9
Selective Expansion of Target Cells Using the Enrich TROVO Platform	9
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	8
Tergitol-15-S-9 Inactivates SARS-CoV-2 and Boosts Immunoassay Signals	8
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	7
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	7
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	7
Techniques to Save the Sea	7
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	7
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage	7
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	7
When is an SNP not an SNP?	6
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	6
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	6
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	6
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	6
Corrigendum	5
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves	5
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	5
X Marks the Spot: Mapping the Brain with EEG and MRI	5
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	5
Urgency of Rapid Legionella Detection Post-COVID-19 Lockdowns: an Interview with Elizabeth Minogue	5
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	5
Extraterrestrial agriculture: plant cultivation in space	5
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	5
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	5
A Simplified Method for Bronchoalveolar Lavage in Mice by Orotracheal Intubation Avoiding Tracheotomy	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	5
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	4
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	4
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	4
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	4
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	4
Who Owns Your DNA?	4
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4