OOIR: Observatory of International Research

Papers

(The TQCC of Biotechniques is 3. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-11-01 to 2024-11-01.)

Article	Citations
Flow Cytometry: Past and Future	41
Adenovirus Vector System: Construction, History and Therapeutic Applications	36
Development of Multiplexed Reverse-Transcription Loop-Mediated Isothermal Amplification for Detection of SARS-CoV-2 and Influenza Viral RNA	35
Cell-Free Protein Synthesis: Advances on Production Process for Biopharmaceuticals and Immunobiological Products	33
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	30
Western Blotting: A Powerful Staple In Scientific and Biomedical Research	26
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	24
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	24
Comparison of Target Enrichment Strategies for Ancient Pathogen DNA	23
A Comparison of DNA/RNA Extraction Protocols for High-Throughput Sequencing of Microbial Communities	21
A Novel Ca ²⁺ Indicator for Long-term Tracking of Intracellular Calcium Flux	19
Agarose Gel Electrophoresis to Assess PCR Product Yield: Comparison with Spectrophotometry, Fluorometry and qPCR	18
Injection of Evans Blue Dye to Fluorescently Label and Image Intact Vasculature	18
Has DeepMind’s AlphaFold Solved the Protein Folding Problem?	16
Western Blotting of Native Proteins from Agarose Gels	15
High-Purity DNA Extraction from Animal Tissue using Picking in the Trizol-Based Method	15
Protocols for the Preparation and Characterization of Decellularized Tissue and Organ Scaffolds for Tissue Engineering	15
Application of a Robust Microplate Assay to Determine Induced β-1,3-glucanase and Chitinase Activity in the Cotton Plant	14
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	13
Evidence-Based Point-of-care Technology Development During the COVID-19 Pandemic	12
Rapid Visual Detection of SARS-CoV-2 by Colorimetric Loop-mediated Isothermal Amplification	12
Comparison of Methods for Mapping Rhizosphere Processes in the Context of Their Surrounding Root and Soil Environments	11
Efficient Multiplexing and Variant Discrimination in Reverse-transcription Loop-mediated Isothermal Amplification with Sequence-Specific Hybridization Probes	11
Increased Sensitivity Using Real-Time dPCR for Detection of SARS-CoV-2	11
Unbiased Automated Quantitation of ROS Signals in Live Retinal Neurons of Drosophila using Fiji/ImageJ	10

Mini-Thermal Platform Integrated with Microfluidic Device with On-Site Detection for Real-Time DNA Amplification	10
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	10
Reverse Complement-PCR, an Innovative and Effective method for Multiplexing Forensically Relevant Single Nucleotide Polymorphism Marker Systems	9
Diagnosis of Genitourinary Tuberculosis by Loop-Mediated Isothermal Amplification Based on SYBR Green I Dye Reaction	9
Design and Characterization of a 3D-printed Staggered Herringbone Mixer	9
Targeted Proteomics Analysis of Plasma Proteins Using Recombinant Protein Standards for Addition Only Workflows	9
Magnetic Tweezers: Development and Use in Single-Molecule Research	9
Dielectrophoretic Characterization of Dendritic Cell Deformability Upon Maturation	8
Auxin-Inducible Degron System: An Efficient Protein Degradation Tool to Study Protein Function	8
Metagenomics: Preventing Future Pandemics	8
eDNA Extraction Protocol for Metagenomic Studies in Tropical Soils	8
A Fast, Nondestructive Method for the Detection of Disease-Related Lesions and Wounded Leaves	7
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	7
A Simple Approach for Effective Shearing and Reliable Concentration Measurement of Ultra-High-Molecular-Weight DNA	7
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	7
pET Expression Vector Customized for Efficient Seamless Cloning	7
Recombinant Bacterial Endotoxin Testing: A Proven Solution	7
Sociometric Wearable Devices for Studying Human Behavior in Corporate and Healthcare Workplaces	7
CRISPR vs COVID-19: How can Gene Editing Help Beat a Virus?	7
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	7
Comparison of DNA Extraction Methods for Samples from Old Blood Collections	6
Isothermal Amplification Using Sequence-Specific Fluorescence Detection of SARS Coronavirus 2 and Variants in Nasal Swabs	6
Optimization of Phosphate-Limited Autoinduction Broth for Two-Stage Heterologous Protein Expression in Escherichia Coli	6
A Method for the Temperature-Controlled Extraction of DNA from Ancient Bones	6
A Clinically Validated Human Saliva Metatranscriptomic Test for Global Systems Biology Studies	6
tANCHOR: A Novel Mammalian Cell Surface Peptide Display System	6
Generation of Genetically Tailored Porcine Liver Cancer Cells by CRISPR/Cas9 Editing	6
Advances of Multiplex Ligation-dependent Probe Amplification Technology in Molecular Diagnostics	5
Quantifying Sequencing Error and Effective Sequencing Depth of Liquid Biopsy NGS with UMI Error Correction	5
An Optimized TRIzol-Based Method for Isolating RNA from Adipose Tissue	5
MILKSHAKE: novel validation method for antibodies to post-translationally modified targets by surrogate Western blot	5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	5
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	5
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	4
Enhanced Electrochemical Measurement of β-galactosidase Activity in Whole Cells by Coexpression of Lactose Permease, LacY	4
Selection and Validation of Reference Genes for Quantitative Real-Time PCR in Cymbidium Sinense	4
Tergitol-15-S-9 Inactivates SARS-CoV-2 and Boosts Immunoassay Signals	4
A High-Quality Fungal Genome Assembly Resolved from a Sample Accidentally Contaminated by Multiple Taxa	4
Design Considerations for Point-of-Need Devices Based on Nucleic Acid Amplification for COVID-19 Diagnostics and Beyond	4
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	4
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	4
Antibodies Validated for Routinely Processed Tissues Stain Frozen Sections Unpredictably	4
Low-cost Modular Systems for Agarose Gel Documentation	4
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	4
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	4
Digital Holographic Microscopy: A Noninvasive Method to Analyze the Formation of Spheroids	4
Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	4
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	4
Bioimprinting: Bringing Together 2D and 3D in Dissecting Cancer Biology	4
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	4

Screening for Androgen Agonists using Autonomously Bioluminescent HEK293 Reporter Cells	4
Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia Pastoris	3
Cytotoxic T Lymphocytes Targeting a Conserved SARS-CoV-2 Spike Epitope are Efficient Serial Killers	3
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	3
An Assay of Human Tyrosine Protein Kinase ABL Activity using an Escherichia Coli Protein Expression System	3
A Low-Cost Platform Suitable for Sequencing-Based Recovery of Natural Variation in Understudied Plants	3
Quantitative ELISA for SERPINA4/kallistatin	3
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	3
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	3
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	3
Reliable Budding Pattern Classification of Yeast Cells with Time-Resolved Measurement of Metabolite Production	3
Adaptation of the HEp-2 Cell Line to toTally Animal-Free Culture Systems and Real-Time Analysis of Cell Growth	3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	3
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	3
Room Temperature in Scientific Protocols and Experiments Should be Defined: a Reproducibility Issue	3
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	3
A Single-wavelength Flow Cytometric Approach using Redox-sensitive Green Fluorescent Protein Probes for Measuring Redox Stress in Live Cells	3
A Simple Method of Drying Polyacrylamide Slab Gels that Eliminates Cracking	3
A Novel Click Chemistry-based Peptide ELISA Protocol: Development and Technical Evaluation	3
Premature Termination Codon: A Tunable Protein Translation Approach	3