OOIR: Observatory of International Research

Papers

(The median citation count of Biotechniques is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-01-01 to 2026-01-01.)

Article	Citations
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	101
Ancient DNA Analysis from Epoxy Resin Biodur ^® -embedded bones	88
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	67
UK Biobank: what can it do, how you can use it and how is it being used?	62
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	51
Correction	36
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	23
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	22
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	20
Rectal swab DNA collection protocol for PCR genotyping in rats	19
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	17
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	16
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	15
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	14
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	14
Education in Microscopy: Taking a Closer Look with Jennifer Waters	14
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	13
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	13
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	12
How has CRISPR Transformed Therapeutic Drug Discovery?	12
Who wants to live forever? Models shaping the future of aging research	11
Gel Purification of gDNA for Next-Generation Sequencing Applications	11
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	9
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage	9
Techniques to Save the Sea	8

Selective Expansion of Target Cells Using the Enrich TROVO Platform	8
When is an SNP not an SNP?	8
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	8
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	8
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	8
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	7
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	7
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	7
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	7
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	7
Corrigendum	6
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	6
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	6
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	6
Correction	6
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	6
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	5
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	5
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	5
Extraterrestrial agriculture: plant cultivation in space	5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves	5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
X Marks the Spot: Mapping the Brain with EEG and MRI	5
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	5
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	5
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	4
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel	4
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital	4
Decoding the Mechanisms of Embryo Development with Spatial Biology	4
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	4
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	4
A simple and rapid in vitro assay for identification of direct inhibitors of O ⁶ -methylguanine-DNA methyltransferase	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Adenovirus Vector System: Construction, History and Therapeutic Applications	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4
A drug screening platform for protein expression levels in neurological disorders	4
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	4
Development of an enhanced anti-pan-N-formylmethionine-specific antibody	3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli	3
Premature Termination Codon: A Tunable Protein Translation Approach	3
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics	3
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	3
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	3

A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein	3
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information	3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection	3
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies	3
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	3
Analytical validation of the IBD segment-based tool KinSNP ^® for human identification applications	3
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	3
Toxoplasma Gondii : The Kitty Litter Parasite	3
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)	3
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis	3
Sampling and analysis methods of air-borne microorganisms in hospital air: a review	3
Microbial Forensics: What We've Learned from Amerithrax and Beyond	3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle	3
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes	3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	3
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis	3
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	3
A Simplified Procedure for Isolation of Primary Murine Microglia	3
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports	3
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues	2
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	2
DNA Extraction from Clotted Blood in Genotyping Quality	2
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	2
Optimized RNA Isolation of FFPE Uterine Scar Tissues for RNA Expression Analyses Delineated by Laser Microdissection	2
Considerations for Colorblind Individuals on Selecting Colorimetric or Fluorescent Dye Assay Outcomes	2
Methodology Established for the Detection of Circulating Tumor DNA by Hybridization Capture	2
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma	2
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	2
Pre-sequencing assessment of RNA-Seq library quality using real-time qPCR	2
Comparison of purification protocols for effective large-primer removal between rounds of PCR amplification	2
The Path to Solving the Protein Folding Problem	2
Novel buffer for long-term preservation of DNA in biological material at room temperature	2
Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research	2
Streamlining single-cell spatial transcriptomics for human kidney tissue	2
Efficient production system for hydrogel-based transparent soil for plant root observation	2
Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	2
Easy Method for Six-Fragment Golden Gate Assembly of Modular Vectors	2
Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations	2
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	2
Cradle Cultures: Growing Stem Cell-Derived Developmental Cell Models in Vitro	2
Taking root: the techniques growing genetically engineered plants	2
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	2
Mapping the spread of antibiotic resistance genes in the coastal microbiome	2
HIV: The Pursuit of an Elusive Vaccine	2
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	2
Parameter optimization of NanoJ-SRRF for live-cell microtubule imaging	2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry	2
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	2
Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate	2
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	2
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	2
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection	2
Comparison of Optimized Methodologies for Isolating Nuclei from Esophageal Tissue	2
Stabilization of human RNA and DNA in stool samples at room temperature	2
Artificial intelligence: help or hindrance in solving the reproducibility crisis?	2
Impact of ambient temperature exposure on miRNA stability in human plasma	2
A Simple and Rapid Method for Isolating High-quality RNA from Kenaf with High Polysaccharide and Polyphenol Contents	2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	2