Biotechniques

Article	Citations
PCR past, present and future	151
Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride	129
Improving RNAi efficiency for pest control in crop species	73
Molecularly imprinted polymers in biological applications	65
Optimization of staining with SYTO 9/propidium iodide: interplay, kinetics and impact on Brevibacillus brevis	45
Antibody validation for protein expression on tissue slides: a protocol for immunohistochemistry	33
Flow cytometry: past and future	30
Genetic molecular markers to accelerate genetic gains in crops	29
Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA	29
RNA interference approaches for plant disease control	27
Cell-free protein synthesis: advances on production process for biopharmaceuticals and immunobiological products	27
Step emulsification: high-throughput production of monodisperse droplets	26
Critical role of three-dimensional tumorsphere size on experimental outcome	25
A comparison of six DNA extraction protocols for 16S, ITS and shotgun metagenomic sequencing of microbial communities	25
Adenovirus vector system: construction, history and therapeutic applications	23
Conventional and innovative methods to assess oxidative stress biomarkers in the clinical cardiovascular setting	22
Phagocytosis assays with different pH-sensitive fluorescent particles and various readouts	22
Contrast-enhanced ultrasound for the assessment of placental development and function	20
Comparison of target enrichment strategies for ancient pathogen DNA	18
Ladder-shape melting temperature isothermal amplification of nucleic acids	17
Real-time loop-mediated isothermal amplification assay for rapid detection of Fusarium circinatum	17
A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities	16
A new approach to epithelial–mesenchymal transition diagnostics in epithelial tumors: double immunofluorescent staining and flow cytometry	15
A novel Ca2+ indicator for long-term tracking of intracellular calcium flux	15
A clinically validated human capillary blood transcriptome test for global systems biology studies	14

A systematic investigation of key factors of nucleic acid precipitation toward optimized DNA/RNA isolation	14
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors	14
Protocols for the preparation and characterization of decellularized tissue and organ scaffolds for tissue engineering	13
Western blotting: a powerful staple in scientific and biomedical research	13
Isolation of extracellular vesicles from intestinal tissue in a mouse model of intestinal ischemia/reperfusion injury	13
2D and 3Din vitroassays to quantify the invasive behavior of glioblastoma stem cells in response to SDF-1α	13
Protocols for accelerated production and purification of collagen scaffold and atelocollagen from animal tissues	12
Agarose gel electrophoresis to assess PCR product yield: comparison with spectrophotometry, fluorometry and qPCR	12
High-purity DNA extraction from animal tissue using picking in the TRIzol-based method	12
Bioprocess intensification: aspirations and achievements	12
Injection of Evans blue dye to fluorescently label and image intact vasculature	12
Has DeepMind's AlphaFold solved the protein folding problem?	12
Rapid visual detection of SARS-CoV-2 by colorimetric loop-mediated isothermal amplification	12
Western blotting of native proteins from agarose gels	11
Unbiased automated quantitation of ROS signals in live retinal neurons of Drosophila using Fiji/ImageJ	10
Automated calibration of optoPlate LEDs to reduce light dose variation in optogenetic experiments	10
Discovery of alliin as a putative inhibitor of the main protease of SARS-CoV-2 by molecular docking	10
Empowering academic labs and scientists to test for COVID-19	10
Evidence-based point-of-care technology development during the COVID-19 pandemic	10
Influence of spore morphology on spectrophotometric quantification of Trichoderma inocula	10
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction	10
TADBD: a sensitive and fast method for detection of typologically associated domain boundaries	9
Analysis of cancer-related mutations in extracellular vesicles RNA by Droplet Digital™ PCR	9
Comparison of methods for mapping rhizosphere processes in the context of their surrounding root and soil environments	9
A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates	9
Advances in single cell technologies in immunology	9
Crystal violet staining is a reliable alternative to bicinchoninic acid assay-based normalization	9
Digital droplet PCR and IDAA for the detection of CRISPR indel edits in the malaria species Anopheles stephensi	8
Developing drugs for the ‘undruggable’	8
Targeted proteomics analysis of plasma proteins using recombinant protein standards for addition only workflows	8
A device for stereotaxic viral delivery into the brains of neonatal mice	8
Optimization of western blotting for the detection of proteins of different molecular weight	8
AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays	8
Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier	8
Application of a robust microplate assay to determine induced β-1,3-glucanase and chitinase activity in the cotton plant	7
Design and characterization of a 3D-printed staggered herringbone mixer	7
Efficient multiplexing and variant discrimination in reverse-transcription loop-mediated isothermal amplification with sequence-specific hybridization probes	7
Nanoadhesive layer to prevent protein absorption in a poly(dimethylsiloxane) microfluidic device	7
Diagnosis of genitourinary tuberculosis by loop-mediated isothermal amplification based on SYBR Green I dye reaction	7
Intracellular flow cytometry complements RT-qPCR detection of circulating SARS-CoV-2 variants of concern	7
Metagenomics: preventing future pandemics	7
Magnetic tweezers: development and use in single-molecule research	7
CRISPR vs COVID-19: how can gene editing help beat a virus?	7
A simple and reliable method for determination of optimum pH in coupled enzyme assays	7
Increased sensitivity using real-time dPCR for detection of SARS-CoV-2	7
Selection of reference genes suitable for normalization of RT-qPCR data in glioma stem cells	7
Effective strategies for isolating DNA from members of Asteraceae with high concentrations of secondary metabolites	7
Empowering chimeric antigen receptor T-cell therapy with CRISPR	6
Western blotting (immunoblotting): history, theory, uses, protocol and problems	6
Methods for simultaneous and quantitative isolation of mitochondrial DNA, nuclear DNA and RNA from mammalian cells	6

eDNA extraction protocol for metagenomic studies in tropical soils	6
Organoid model of urothelial cancer: establishment and applications for bladder cancer research	6
Recombinant bacterial endotoxin testing: a proven solution	6
A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue	6
Automated epidermal thickness quantification of in vitro human skin equivalents using optical coherence tomography	6
Various factors affect lipopolysaccharide sensitization in cell cultures	6
pET expression vector customized for efficient seamless cloning	6
Seeing the full picture: advances in 3D cell culture microscopy	6
Reverse complement-PCR, an innovative and effective method for multiplexing forensically relevant single nucleotide polymorphism marker systems	6
Cellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform	6
Single-particle chemical force microscopy to characterize virus surface chemistry	6
A method for the temperature-controlled extraction of DNA from ancient bones	6
A clinically validated human saliva metatranscriptomic test for global systems biology studies	6
A simple approach for effective shearing and reliable concentration measurement of ultra-high-molecular-weight DNA	6
Emulsion PCR made easy	5
Dielectrophoretic characterization of dendritic cell deformability upon maturation	5
Generation of genetically tailored porcine liver cancer cells by CRISPR/Cas9 editing	5
A fast, nondestructive method for the detection of disease-related lesions and wounded leaves	5
A simple serum depletion method for proteomics analysis	5
Development of a Droplet Digital™ PCR DNA methylation detection and quantification assay of prenatal tobacco exposure	5
Effect of different cloning strategies in pET-28a on solubility and functionality of a staphylococcal phage endolysin	5
tANCHOR: a novel mammalian cell surface peptide display system	5
Quantifying sequencing error and effective sequencing depth of liquid biopsy NGS with UMI error correction	5
Application of the Gyrolab microfluidic platform to measure picomolar affinity of a PD-L1-binding Adnectin™ radioligand for positron emission tomography	5
A novel decatenation assay for DNA topoisomerases using a singly-linked catenated substrate	4
Methods to evaluate the impact of SARS-CoV-2 nucleocapsid mutations on antigen detection by rapid diagnostic tests	4
Kinase activity-tagged western blotting assay	4
Non-destructive extraction of DNA from preserved tissues in medical collections	4
Sociometric wearable devices for studying human behavior in corporate and healthcare workplaces	4
Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases	4
Isolation of DNA-free RNA from human bone marrow mononuclear cells: comparison of laboratory methods	4
Noninvasive prenatal diagnosis of hemophilia A by a haplotype-based approach using cell-free fetal DNA	4
Mini-thermal platform integrated with microfluidic device with on-site detection for real-time DNA amplification	4
An optimized procedure for quantitative analysis of mitophagy with the mtKeima system using flow cytometry	4
A method for isolating RNA from canine bone	4
Target sequence heterogeneity causes the ‘hook effect’ in fluorescent dye-based quantitative PCR	4
Isothermal amplification using sequence-specific fluorescence detection of SARS coronavirus 2 and variants in nasal swabs	4
SUMOylation of the chromodomain factor MRG-1 inC. elegansaffects chromatin-regulatory dynamics	4
An engraved surface induces weak adherence and high proliferation of nonadherent cells and microorganisms during culture	4
Analytical validation of an error-corrected ultra-sensitive ctDNA next-generation sequencing assay	4
MILKSHAKE: novel validation method for antibodies to post-translationally modified targets by surrogate Western blot	4
Optimization of phosphate-limited autoinduction broth for two-stage heterologous protein expression in Escherichia coli	4
Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000	4
A high-quality fungal genome assembly resolved from a sample accidentally contaminated by multiple taxa	4
Detection and differentiation of bacteria by electrical bioimpedance spectroscopy	4
Improved third-generation lentiviral packaging with pLKO.1C vectors	4
Advances of multiplex ligation-dependent probe amplification technology in molecular diagnostics	4
Reduced serum methods for contact-based coculture of human dermal fibroblasts and epidermal keratinocytes	4
Quantitative immunohistochemistry using an antibody-fused bioluminescent protein	4
CRISPR-PN2: a flexible and genome-aware platform for diverse CRISPR experiments in parasitic nematodes	4
Expression of mouse small interfering RNAs in lettuce using artificial microRNA technology	4
Comparison of DNA extraction methods for samples from old blood collections	4
A simple method of drying polyacrylamide slab gels that eliminates cracking	3
An assay of human tyrosine protein kinase ABL activity using an Escherichia coli protein expression system	3
Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters	3
Apoptosis assessment in high-content and high-throughput screening assays	3
An improved gene synthesis method with asymmetric directions of oligonucleotides designed using a simulation program	3
Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris	3
Effects of myofiber isolation technique on sarcolemma biomechanics	3
Room temperature in scientific protocols and experiments should be defined: a reproducibility issue	3
Colonoscopy-based intramucosal transplantation of cancer cells for mouse modeling of colon cancer and lung metastasis	3
Strand-specific detection of overlapping transcripts via purification involving denaturation of biotinylated cDNA	3
Metagenomic nanopore sequencing versus conventional diagnosis for identification of the dieback pathogens of mango trees	3
DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen	3
A novel flow cytometry-based quantitative monocyte adhesion assay to estimate endothelial cell activation in vitro	3
A novel click chemistry-based peptide ELISA protocol: development and technical evaluation	3
Selection and validation of reference genes for quantitative real-time PCR in Cymbidium sinense	3
Yielding quality viral RNA by using two different chemistries: a comparative performance study	3
Cytotoxic T lymphocytes targeting a conserved SARS-CoV-2 spike epitope are efficient serial killers	3
Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension	3
Auxin-inducible degron system: an efficient protein degradation tool to study protein function	3
Cellular thermal shift analysis for interrogation of CRISPR-assisted proteomic changes	3
Reliable budding pattern classification of yeast cells with time-resolved measurement of metabolite production	3
A single-wavelength flow cytometric approach using redox-sensitive green fluorescent protein probes for measuring redox stress in live cells	3
Digital holographic microscopy: a noninvasive method to analyze the formation of spheroids	3
Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth	3
Premature termination codon: a tunable protein translation approach	3
Evaluation of DNA degradation and establishment of a degradation analysis model for Lepidoptera specimens	3
Direct-current electric field stimulation promotes proliferation and maintains stemness of mesenchymal stem cells	3
Low-cost modular systems for agarose gel documentation	3

A method combining TA cloning and fluorescence screening for rapid acquisition of transgenic seeds	3
Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform	2
Investigation of circulating DNA integrity after blood collection	2
Bioimprinting: bringing together 2D and 3D in dissecting cancer biology	2
Overcoming problems to produce the recombinant protein LipL21 of Leptospira interrogans	2
Intratracheal aerosolization of viral vectors to newborn pig airways	2
The determination of cystatin C in biological samples via the surface plasmon resonance method	2
How has the COVID-19 pandemic impacted PCR?	2
Cloning vectors for gene delivery, integration and expression in Campylobacter jejuni	2
Preparation of Bacillus subtilis cell samples and generation of an SDS-PAGE	2
SARS-CoV-2 spike gene Sanger sequencing methodology to identify variants of concern	2
Antibodies validated for routinely processed tissues stain frozen sections unpredictably	2
High-sensitivity microvolume UV absorption spectrometry for routine analysis of small-volume biological samples	2
In vitro amplification of whole large plasmids via transposon-mediated oriC insertion	2
A rapid, nondestructive method for vascular network visualization	2
Quality control and statistical evaluation of combinatorial DNA libraries using nanopore sequencing	2
COVID-19: How has the scientific community risen to the challenge?	2
Inside the brain of a killer: the ethics of neuroimaging in a criminal conviction	2
Screening for androgen agonists using autonomously bioluminescent HEK293 reporter cells	2
Influence of resuspension volume on dry sampling devices taken for human papillomavirus testing: implications for self-sampling	2
Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene	2
New cloning vectors to facilitate quick allelic exchange in gram-negative bacteria	2
An online tool for fetal fraction prediction based on direct size distribution analysis of maternal cell-free DNA	2
Inhaler with electrostatic sterilizer and use of cationic amphiphilic peptides may accelerate recovery from COVID-19	2
Enhanced electrochemical measurement of β-galactosidase activity in whole cells by coexpression of lactose permease, LacY	2
Optimized RNA isolation of FFPE uterine scar tissues for RNA expression analyses delineated by laser microdissection	2
A modified method for efficient RNA isolation from mangrove root tissues rich in secondary metabolites	2
Robust co-immunoprecipitation with mass spectrometry for Caenorhabditis elegans using solid-phase enhanced sample preparation	2
An irew1 mutant intensifies lateral root branching for adaption upon water stress	2
A building block-based beam-break (B5) locomotor activity monitoring system and its use in circadian biology research	2
Tergitol-15-S-9 inactivates SARS-CoV-2 and boosts immunoassay signals	2
Examination of vesicular stomatitis virus-induced morphology changes in individual Vero cells by QMod microscopy	2
ncRNAseq: simple modifications to RNA-seq library preparation allow recovery and analysis of mid-sized non-coding RNAs	2
Democratizing bioinformatics through easily accessible software platforms for non-experts in the field	2
A compact and simple method of achieving differential transgene expression by exploiting translational readthrough	2
Accelerated aging of forensically relevant biological materials on swabs	2
Design considerations for point-of-need devices based on nucleic acid amplification for COVID-19 diagnostics and beyond	2
3D-printed assistive pipetting system for gel electrophoresis for technicians with low acuity vision	2
Big data, big future	2
Antibody-mediated soluble CD14 stabilization prevents agitation-induced increases in presepsin levels in blood component specimens	2
Modeling performance of sample collection sites using whole exome sequencing metrics	2
The ABCs of reproducibility: effecting attitudes, behaviors and change to realize reproducibility	2
Quantitative ELISA for SERPINA4/kallistatin	2
Optimized methods for IL-17A refolding and anti-IL17A Fab production for co-crystallization with small molecules	2
A cell-based assay for the detection of neutralizing antibodies against alemtuzumab	2
A dextran-based warming method for preparing leukocyte-rich plasma and its clinical application for endotoxin assay	2