Biotechniques

Article	Citations
UK Biobank: what can it do, how you can use it and how is it being used?	76
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	73
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones	56
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	53
Rectal swab DNA collection protocol for PCR genotyping in rats	44
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	31
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	21
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	19
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	16
Welcome to the 72nd Volume of BioTechniques	15
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	15
Correction	15
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	14
Education in Microscopy: Taking a Closer Look with Jennifer Waters	13
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	13
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	12
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	11
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	11
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	11
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	10
The Forensic Genomics Toolbox is Expanding	9
Improved SARS-CoV-2 PCR Detection and Genotyping with Double-Bubble Primers	9
Gel Purification of gDNA for Next-Generation Sequencing Applications	9
Selective Expansion of Target Cells Using the Enrich TROVO Platform	9
How has CRISPR Transformed Therapeutic Drug Discovery?	9

Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	8
Who wants to live forever? Models shaping the future of aging research	8
Techniques to Save the Sea	7
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage	7
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	7
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	7
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	7
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	6
When is an SNP not an SNP?	6
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	6
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	6
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	6
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	6
Corrigendum	5
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	5
Extraterrestrial agriculture: plant cultivation in space	5
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	5
A Simplified Method for Bronchoalveolar Lavage in Mice by Orotracheal Intubation Avoiding Tracheotomy	5
Urgency of Rapid Legionella Detection Post-COVID-19 Lockdowns: an Interview with Elizabeth Minogue	5
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves	5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	4
X Marks the Spot: Mapping the Brain with EEG and MRI	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	4
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	4
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	4
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	4
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	4
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	4
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel	3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle	3
Adenovirus Vector System: Construction, History and Therapeutic Applications	3
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	3
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital	3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	3
A Simplified Procedure for Isolation of Primary Murine Microglia	3
Development of an enhanced anti-pan-N-formylmethionine-specific antibody	3
Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications	3
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	3
A drug screening platform for protein expression levels in neurological disorders	3

A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase	3
Decoding the Mechanisms of Embryo Development with Spatial Biology	3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli	3
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein	3
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)	3
Premature Termination Codon: A Tunable Protein Translation Approach	3
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	3
Psychobiotics: Are we on the Cusp of a Breakthrough?	3
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	3
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	3
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies	3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection	3
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics	3
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes	3
Microbial Forensics: What We've Learned from Amerithrax and Beyond	3
Parameter optimization of NanoJ-SRRF for live-cell microtubule imaging	2
Stabilization of human RNA and DNA in stool samples at room temperature	2
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	2
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	2
The Path to Solving the Protein Folding Problem	2
Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research	2
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	2
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	2
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports	2
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information	2
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	2
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma	2
DNA Extraction from Clotted Blood in Genotyping Quality	2
Mapping the spread of antibiotic resistance genes in the coastal microbiome	2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	2
Toxoplasma Gondii : The Kitty Litter Parasite	2
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	2
Sampling and analysis methods of air-borne microorganisms in hospital air: a review	2
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis	2
Novel buffer for long-term preservation of DNA in biological material at room temperature	2
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues	2
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection	2
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry	2
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	2
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	2
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis	2
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	1
A single-cell 3D dynamic volume control system for chondrocytes	1
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	1
Considerations for Developing Mitochondrial Transplantation Techniques for Individualized Medicine	1
Sample Collection/Stabilization and DNA/RNA Extraction from Swab Samples for Microbiome or Metagenome Analyses	1
Mini-Thermal Platform Integrated with Microfluidic Device with On-Site Detection for Real-Time DNA Amplification	1
Functionalized primer initiated signal cycles and personal glucose meter for sensitive and portable miRNA analysis	1
A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research	1
A Simple and Rapid Method for Isolating High-quality RNA from Kenaf with High Polysaccharide and Polyphenol Contents	1
Lab Techniques for a More Sustainable World	1
Comparison of Optimized Methodologies for Isolating Nuclei from Esophageal Tissue	1
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	1
MSCProfiler: A Single Cell Image Processing Workflow to Investigate Mesenchymal Stem Cell Heterogeneity	1
HIV: The Pursuit of an Elusive Vaccine	1
Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	1
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	1
Wheat Improvement Using Genome Editing Technology	1
Cassava for the future: embryogenic liquid cultures suitable for new biotech techniques	1
Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate	1
Magnetic Tweezers: Development and Use in Single-Molecule Research	1
Flow Cytometry: Past and Future	1
ncRNAseq: Simple Modifications to RNA-Seq Library Preparation Allow Recovery and Analysis of Mid-Sized Non-Coding RNAs	1
Methodology Established for the Detection of Circulating Tumor DNA by Hybridization Capture	1
How is Spatial Transcriptomics Influencing Cancer Research and Diagnostics?	1
Easy Method for Six-Fragment Golden Gate Assembly of Modular Vectors	1
Cytotoxic T Lymphocytes Targeting a Conserved SARS-CoV-2 Spike Epitope are Efficient Serial Killers	1
Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations	1
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	1
Efficient production system for hydrogel-based transparent soil for plant root observation	1
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	1
Cradle Cultures: Growing Stem Cell-Derived Developmental Cell Models in Vitro	1
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	1
Accurate, Fast and Cost-Effective Simultaneous Detection of Bacterial Meningitis by Qualitative PCR with High-Resolution Melting	1
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	1
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	1
A novel device for buffy coat collection	1
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	1

Recording the Brain in Vivo : Emerging Technologies for the Exploration of Mental Health Conditions	1
Comparison of Methods for Mapping Rhizosphere Processes in the Context of Their Surrounding Root and Soil Environments	1
An enzyme-free alcohol-based organoid harvesting solution	1
Adipocyte ABCA1 expression analysis using flow cytometry	1
Corrigendum	1
Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	1
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	1
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	1
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	1
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	1
Extracting High-Quality RNA From Formaldehyde-Fixed Naturally Aged Neuromusculoskeletal Tissues	1
Considerations for Colorblind Individuals on Selecting Colorimetric or Fluorescent Dye Assay Outcomes	1
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	1
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	1
Artificial intelligence: help or hindrance in solving the reproducibility crisis?	1
Dual compartment utility of BRET-based biosensors for PPP2R5A/B56α, a cancer-associated B regulatory subunit of PP2A	1
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	1
Optimized RNA Isolation of FFPE Uterine Scar Tissues for RNA Expression Analyses Delineated by Laser Microdissection	1
High-Yield Expression of Periplasmic Single-Chain Variable Fragments by Solid Escherichia Coli Cultures	1
Removal of Nonspecific Binding Proteins is Required in Co-Immunoprecipitation with Nuclear Proteins	1
Advances of Multiplex Ligation-dependent Probe Amplification Technology in Molecular Diagnostics	1
Effect of Mismatch Between Types of Viral Nucleic Acid and Intended Targets of Extraction Kits on Polymerase Chain Reaction-Based Testing	1