Biotechniques

Article	Citations
Flow Cytometry: Past and Future	41
Adenovirus Vector System: Construction, History and Therapeutic Applications	36
Development of Multiplexed Reverse-Transcription Loop-Mediated Isothermal Amplification for Detection of SARS-CoV-2 and Influenza Viral RNA	35
Cell-Free Protein Synthesis: Advances on Production Process for Biopharmaceuticals and Immunobiological Products	33
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	30
Western Blotting: A Powerful Staple In Scientific and Biomedical Research	26
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	24
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	24
Comparison of Target Enrichment Strategies for Ancient Pathogen DNA	23
A Comparison of DNA/RNA Extraction Protocols for High-Throughput Sequencing of Microbial Communities	21
A Novel Ca 2+ Indicator for Long-term Tracking of Intracellular Calcium Flux	19
Agarose Gel Electrophoresis to Assess PCR Product Yield: Comparison with Spectrophotometry, Fluorometry and qPCR	18
Injection of Evans Blue Dye to Fluorescently Label and Image Intact Vasculature	18
Has DeepMind’s AlphaFold Solved the Protein Folding Problem?	16
Western Blotting of Native Proteins from Agarose Gels	15
High-Purity DNA Extraction from Animal Tissue using Picking in the Trizol-Based Method	15
Protocols for the Preparation and Characterization of Decellularized Tissue and Organ Scaffolds for Tissue Engineering	15
Application of a Robust Microplate Assay to Determine Induced β-1,3-glucanase and Chitinase Activity in the Cotton Plant	14
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	13
Evidence-Based Point-of-care Technology Development During the COVID-19 Pandemic	12
Rapid Visual Detection of SARS-CoV-2 by Colorimetric Loop-mediated Isothermal Amplification	12
Efficient Multiplexing and Variant Discrimination in Reverse-transcription Loop-mediated Isothermal Amplification with Sequence-Specific Hybridization Probes	11
Increased Sensitivity Using Real-Time dPCR for Detection of SARS-CoV-2	11
Comparison of Methods for Mapping Rhizosphere Processes in the Context of Their Surrounding Root and Soil Environments	11
Mini-Thermal Platform Integrated with Microfluidic Device with On-Site Detection for Real-Time DNA Amplification	10

Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	10
Unbiased Automated Quantitation of ROS Signals in Live Retinal Neurons of Drosophila using Fiji/ImageJ	10
Reverse Complement-PCR, an Innovative and Effective method for Multiplexing Forensically Relevant Single Nucleotide Polymorphism Marker Systems	9
Diagnosis of Genitourinary Tuberculosis by Loop-Mediated Isothermal Amplification Based on SYBR Green I Dye Reaction	9
Design and Characterization of a 3D-printed Staggered Herringbone Mixer	9
Targeted Proteomics Analysis of Plasma Proteins Using Recombinant Protein Standards for Addition Only Workflows	9
Magnetic Tweezers: Development and Use in Single-Molecule Research	9
Dielectrophoretic Characterization of Dendritic Cell Deformability Upon Maturation	8
Auxin-Inducible Degron System: An Efficient Protein Degradation Tool to Study Protein Function	8
Metagenomics: Preventing Future Pandemics	8
eDNA Extraction Protocol for Metagenomic Studies in Tropical Soils	8
A Fast, Nondestructive Method for the Detection of Disease-Related Lesions and Wounded Leaves	7
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	7
A Simple Approach for Effective Shearing and Reliable Concentration Measurement of Ultra-High-Molecular-Weight DNA	7
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	7
pET Expression Vector Customized for Efficient Seamless Cloning	7
Recombinant Bacterial Endotoxin Testing: A Proven Solution	7
Sociometric Wearable Devices for Studying Human Behavior in Corporate and Healthcare Workplaces	7
CRISPR vs COVID-19: How can Gene Editing Help Beat a Virus?	7
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	7
Comparison of DNA Extraction Methods for Samples from Old Blood Collections	6
Isothermal Amplification Using Sequence-Specific Fluorescence Detection of SARS Coronavirus 2 and Variants in Nasal Swabs	6
Optimization of Phosphate-Limited Autoinduction Broth for Two-Stage Heterologous Protein Expression in Escherichia Coli	6
A Method for the Temperature-Controlled Extraction of DNA from Ancient Bones	6
A Clinically Validated Human Saliva Metatranscriptomic Test for Global Systems Biology Studies	6
tANCHOR: A Novel Mammalian Cell Surface Peptide Display System	6
Generation of Genetically Tailored Porcine Liver Cancer Cells by CRISPR/Cas9 Editing	6
Quantifying Sequencing Error and Effective Sequencing Depth of Liquid Biopsy NGS with UMI Error Correction	5
An Optimized TRIzol-Based Method for Isolating RNA from Adipose Tissue	5
MILKSHAKE: novel validation method for antibodies to post-translationally modified targets by surrogate Western blot	5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	5
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	5
Advances of Multiplex Ligation-dependent Probe Amplification Technology in Molecular Diagnostics	5
Enhanced Electrochemical Measurement of β-galactosidase Activity in Whole Cells by Coexpression of Lactose Permease, LacY	4
Selection and Validation of Reference Genes for Quantitative Real-Time PCR in Cymbidium Sinense	4
Tergitol-15-S-9 Inactivates SARS-CoV-2 and Boosts Immunoassay Signals	4
A High-Quality Fungal Genome Assembly Resolved from a Sample Accidentally Contaminated by Multiple Taxa	4
Design Considerations for Point-of-Need Devices Based on Nucleic Acid Amplification for COVID-19 Diagnostics and Beyond	4
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	4
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	4
Antibodies Validated for Routinely Processed Tissues Stain Frozen Sections Unpredictably	4
Low-cost Modular Systems for Agarose Gel Documentation	4
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	4
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	4
Digital Holographic Microscopy: A Noninvasive Method to Analyze the Formation of Spheroids	4
Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	4
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	4
Bioimprinting: Bringing Together 2D and 3D in Dissecting Cancer Biology	4
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	4
Screening for Androgen Agonists using Autonomously Bioluminescent HEK293 Reporter Cells	4

Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	3
Room Temperature in Scientific Protocols and Experiments Should be Defined: a Reproducibility Issue	3
An Assay of Human Tyrosine Protein Kinase ABL Activity using an Escherichia Coli Protein Expression System	3
A Low-Cost Platform Suitable for Sequencing-Based Recovery of Natural Variation in Understudied Plants	3
Premature Termination Codon: A Tunable Protein Translation Approach	3
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	3
Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia Pastoris	3
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	3
Reliable Budding Pattern Classification of Yeast Cells with Time-Resolved Measurement of Metabolite Production	3
Adaptation of the HEp-2 Cell Line to toTally Animal-Free Culture Systems and Real-Time Analysis of Cell Growth	3
Quantitative ELISA for SERPINA4/kallistatin	3
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	3
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	3
A Single-wavelength Flow Cytometric Approach using Redox-sensitive Green Fluorescent Protein Probes for Measuring Redox Stress in Live Cells	3
A Simple Method of Drying Polyacrylamide Slab Gels that Eliminates Cracking	3
A Novel Click Chemistry-based Peptide ELISA Protocol: Development and Technical Evaluation	3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	3
Cytotoxic T Lymphocytes Targeting a Conserved SARS-CoV-2 Spike Epitope are Efficient Serial Killers	3
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	3
Gel Purification of gDNA for Next-Generation Sequencing Applications	2
The Forensic Genomics Toolbox is Expanding	2
A Practical, Low-cost, Short-term Storage Method for Genomic DNA	2
DNA Extraction from Clotted Blood in Genotyping Quality	2
Investigation of Circulating DNA Integrity After Blood Collection	2
An Online Tool for Fetal Fraction Prediction Based on Direct Size Distribution Analysis of Maternal Cell-Free DNA	2
Accelerated Aging of Forensically Relevant Biological Materials on Swabs	2
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	2
SARS-CoV-2 Spike Gene Sanger Sequencing Methodology to Identify Variants of Concern	2
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	2
Modified ARCHITECT ® Serologic Assays Enable Plasma-level Performance from Dried Blood spot samples	2
A Modified Method for Efficient RNA Isolation from Mangrove Root Tissues Rich in Secondary Metabolites	2
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	2
Predicting Molecular Docking of Per- and Polyfluoroalkyl Substances to Blood Protein Using Generative Artificial Intelligence Algorithm Diffdock	2
ncRNAseq: Simple Modifications to RNA-Seq Library Preparation Allow Recovery and Analysis of Mid-Sized Non-Coding RNAs	2
Accurate, Fast and Cost-Effective Simultaneous Detection of Bacterial Meningitis by Qualitative PCR with High-Resolution Melting	2
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	2
Overcoming Problems to Produce the Recombinant Protein LipL21 of Leptospira interrogans	2
How has the COVID-19 Pandemic Impacted PCR?	2
Optimized RNA Isolation of FFPE Uterine Scar Tissues for RNA Expression Analyses Delineated by Laser Microdissection	2
Cloning Vectors for Gene Delivery, Integration and Expression in Campylobacter Jejuni	2
High-Quality Total RNA Extraction from Early-Stage Lamprey Embryos	2
High-sensitivity Microvolume UV Absorption Spectrometry for Routine Analysis of Small-volume Biological Samples	2
Antibody-mediated Soluble CD14 Stabilization Prevents Agitation-Induced Increases in Presepsin Levels in Blood Component Specimens	2
The Fluorescent Protein Stability Assay: An Efficient Method For Monitoring Intracellular Protein Stability	2
Selective Expansion of Target Cells Using the Enrich TROVO Platform	2
Inside the Brain of a Killer: The Ethics of Neuroimaging in a Criminal Conviction	2
Colorimetric miRNA Detection Based on Self-Primer-Initiated CRISPR-Cas12a-Assisted Amplification	2
In Vitro Amplification of Whole Large Plasmids Via Transposon-Mediated oriC Insertion	2
A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research	2
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	2
The Determination of Cystatin C in Biological Samples via the Surface Plasmon Resonance Method	2
New Cloning Vectors to Facilitate Quick Allelic Exchange in Gram-Negative Bacteria	2
Easy Method for Six-Fragment Golden Gate Assembly of Modular Vectors	2
3D-Printed Assistive Pipetting System for Gel Electrophoresis for Technicians with Low Acuity Vision	2
Removal of Nonspecific Binding Proteins is Required in Co-Immunoprecipitation with Nuclear Proteins	2
Robust Co-Immunoprecipitation with Mass Spectrometry for Caenorhabditis Elegans Using Solid-Phase Enhanced Sample Preparation	2
CRISPR-PN2: A Flexible and Genome-Aware Platform for Diverse CRISPR Experiments in Parasitic Nematodes	2
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	1
The Path to Solving the Protein Folding Problem	1
Management of Inadvertent Template Contamination in Production of Oligonucleotide qPCR Reagents	1
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	1
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	1
Sensitive and Specific Quantification of Antisense Oligonucleotides Using Probe Alteration-linked Self-assembly Reaction Technology	1
A Simplified Method for Bronchoalveolar Lavage in Mice by Orotracheal Intubation Avoiding Tracheotomy	1
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	1
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli	1
Functionalized primer initiated signal cycles and personal glucose meter for sensitive and portable miRNA analysis	1
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	1
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	1
Beyond Brightfield: A Possible Future of Slide Scanners	1
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	1
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	1
Urgency of Rapid Legionella Detection Post-COVID-19 Lockdowns: an Interview with Elizabeth Minogue	1
Considerations for Developing Mitochondrial Transplantation Techniques for Individualized Medicine	1
Stay Smart: It’s all about the Materials for 4D Printing in Biomedicine	1
MSCProfiler: A Single Cell Image Processing Workflow to Investigate Mesenchymal Stem Cell Heterogeneity	1
Assigning Immunoglobulin Class from Single-Cell Transcriptomes in IgA1-Secreting Versus Membrane Subpopulations	1
Single-cell Sequencing: The Technological Revolution Behind a New Wave of Multiomic Studies in Basic and Cancer Research	1

How is Spatial Transcriptomics Influencing Cancer Research and Diagnostics?	1
Assay System for Mesocotyl Elongation and Hydrotropism of Maize Primary Root in Response to Low Moisture Gradient	1
The Internet of Things for Combatting Antimicrobial Resistance	1
Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d	1
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies	1
Multiscale Computational Modeling Offers Key to Understanding Molecular Logic Underpinning Development and Disease	1
Automated Method for Quantification of 20 Amino Acids in Cell Culture Media During Biopharmaceutical Development	1
A Practical Approach to Genome Assembly and Annotation of Basidiomycota Using the Example of Armillaria	1
Real-time PCR Method for Detection of Short DNA using a Deoxyuridine Probe and Application for Detection of Fomivirsen	1
The Influence of Sample Quantity and Lysis Parameters on the Success of Ancient DNA Extraction from Skeletal Remains	1
Effects of a Fully Enclosed Hollow-Fiber Centrifugal Ultrafiltration Technique for Laboratory Biosafety Improvement	1
How has CRISPR Transformed Therapeutic Drug Discovery?	1
Single-Cell RNA Sequencing of Intramedullary Canal Tissue to Improve Methods for Studying Fracture Repair Biology	1
Building a baby: Are Stem Cell-Based, Embryo-Like Models the Key to Unlocking the Secrets of Human Development?	1
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis	1
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	1
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	1
Is Space the Final Frontier for Mitochondrial Study?	1
Comparison of Optimized Methodologies for Isolating Nuclei from Esophageal Tissue	1
Who Owns Your DNA?	1
Education in Microscopy: Taking a Closer Look with Jennifer Waters	1
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	1
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	1
Effect of Mismatch Between Types of Viral Nucleic Acid and Intended Targets of Extraction Kits on Polymerase Chain Reaction-Based Testing	1
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics	1
An Approach to Identifying Quality Research Antibodies	1
The Root Microbiome: Techniques for Exploration and Agricultural Applications	1
Playback Cloning: Simple, Reversible, Cost-effective Cloning for the Combinatorial Assembly of Complex Expression Constructs	1
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	1
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	1
Reproducibility, Scientific Rigor and Responsibility	1
Unraveling Crime Scenes Strand by Strand: The Forensic Odyssey of Bruce Budowle	1
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	1
High-Yield Expression of Periplasmic Single-Chain Variable Fragments by Solid Escherichia Coli Cultures	1
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	1
Development of an in Vitro Assay for the Detection of Polymerization of the Pyrin Domain of ASC	1
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	1
Comparative in situ Hybridization Protocols in Zebrafish	1
A High-throughput Pipeline for DNA/RNA/small RNA Purification from Tissue Samples for Sequencing	1