Biotechniques

Article	Citations
UK Biobank: what can it do, how you can use it and how is it being used?	84
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	84
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones	60
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	58
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	47
Correction	34
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	22
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	21
Rectal swab DNA collection protocol for PCR genotyping in rats	19
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	18
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	17
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	15
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	15
Welcome to the 72nd Volume of BioTechniques	14
Education in Microscopy: Taking a Closer Look with Jennifer Waters	14
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	13
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	13
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	13
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	12
How has CRISPR Transformed Therapeutic Drug Discovery?	11
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	11
Who wants to live forever? Models shaping the future of aging research	9
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	9
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	9
Gel Purification of gDNA for Next-Generation Sequencing Applications	9

The Forensic Genomics Toolbox is Expanding	8
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	8
Selective Expansion of Target Cells Using the Enrich TROVO Platform	8
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage	8
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	7
Techniques to Save the Sea	7
When is an SNP not an SNP?	7
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	7
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	7
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	6
Correction	6
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	6
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	6
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	6
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	6
Corrigendum	6
X Marks the Spot: Mapping the Brain with EEG and MRI	5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves	5
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	5
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	5
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	5
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	5
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	5
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4
Extraterrestrial agriculture: plant cultivation in space	4
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	4
Adenovirus Vector System: Construction, History and Therapeutic Applications	4
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	4
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Development of an enhanced anti-pan-N-formylmethionine-specific antibody	3
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information	3
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis	3
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)	3
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports	3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli	3
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	3
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel	3
A drug screening platform for protein expression levels in neurological disorders	3
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	3
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes	3
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics	3

Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications	3
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein	3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	3
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle	3
Premature Termination Codon: A Tunable Protein Translation Approach	3
A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase	3
Microbial Forensics: What We've Learned from Amerithrax and Beyond	3
Sampling and analysis methods of air-borne microorganisms in hospital air: a review	3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection	3
A Simplified Procedure for Isolation of Primary Murine Microglia	3
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis	3
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	3
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies	3
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital	3
Decoding the Mechanisms of Embryo Development with Spatial Biology	3
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	2
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry	2
DNA Extraction from Clotted Blood in Genotyping Quality	2
Artificial intelligence: help or hindrance in solving the reproducibility crisis?	2
HIV: The Pursuit of an Elusive Vaccine	2
Comparison of Optimized Methodologies for Isolating Nuclei from Esophageal Tissue	2
Taking root: the techniques growing genetically engineered plants	2
Parameter optimization of NanoJ-SRRF for live-cell microtubule imaging	2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	2
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	2
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	2
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	2
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	2
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	2
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	2
Stabilization of human RNA and DNA in stool samples at room temperature	2
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma	2
Novel buffer for long-term preservation of DNA in biological material at room temperature	2
Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research	2
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	2
Toxoplasma Gondii : The Kitty Litter Parasite	2
The Path to Solving the Protein Folding Problem	2
Mapping the spread of antibiotic resistance genes in the coastal microbiome	2
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	2
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	2
Impact of ambient temperature exposure on miRNA stability in human plasma	2
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection	2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	2
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues	2
A Novel Use of a Needle-free Injection System for Improved Nucleic Acid Delivery and Expression in vivo	1
Validation of CRISPR Targeting for Proliferation and Cytarabine Resistance Control Genes in the Acute Myeloid Leukemia Cell Line MOLM-13	1
Adipocyte ABCA1 expression analysis using flow cytometry	1
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	1
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	1
Effect of Mismatch Between Types of Viral Nucleic Acid and Intended Targets of Extraction Kits on Polymerase Chain Reaction-Based Testing	1
Extracting High-Quality RNA From Formaldehyde-Fixed Naturally Aged Neuromusculoskeletal Tissues	1
Cassava for the future: embryogenic liquid cultures suitable for new biotech techniques	1
Functionalized primer initiated signal cycles and personal glucose meter for sensitive and portable miRNA analysis	1
Wheat Improvement Using Genome Editing Technology	1
Dual compartment utility of BRET-based biosensors for PPP2R5A/B56α, a cancer-associated B regulatory subunit of PP2A	1
Easy Method for Six-Fragment Golden Gate Assembly of Modular Vectors	1
Considerations for Developing Mitochondrial Transplantation Techniques for Individualized Medicine	1
Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations	1
How is Spatial Transcriptomics Influencing Cancer Research and Diagnostics?	1
Cradle Cultures: Growing Stem Cell-Derived Developmental Cell Models in Vitro	1
How do you measure the success and impact of a core facility?	1
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	1
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	1
Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d	1
Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	1
An Optimized TRIzol-Based Method for Isolating RNA from Adipose Tissue	1
Selection and Validation of Reference Genes for Quantitative Real-Time PCR in Cymbidium Sinense	1
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	1
Recording the Brain in Vivo : Emerging Technologies for the Exploration of Mental Health Conditions	1
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	1
Magnetic Tweezers: Development and Use in Single-Molecule Research	1
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	1
A single-cell 3D dynamic volume control system for chondrocytes	1
Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	1
Optimized RNA Isolation of FFPE Uterine Scar Tissues for RNA Expression Analyses Delineated by Laser Microdissection	1
Accurate, Fast and Cost-Effective Simultaneous Detection of Bacterial Meningitis by Qualitative PCR with High-Resolution Melting	1
Considerations for Colorblind Individuals on Selecting Colorimetric or Fluorescent Dye Assay Outcomes	1
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	1

Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	1
ncRNAseq: Simple Modifications to RNA-Seq Library Preparation Allow Recovery and Analysis of Mid-Sized Non-Coding RNAs	1
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	1
Mini-Thermal Platform Integrated with Microfluidic Device with On-Site Detection for Real-Time DNA Amplification	1
High-Yield Expression of Periplasmic Single-Chain Variable Fragments by Solid Escherichia Coli Cultures	1
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	1
Western Blotting: A Powerful Staple In Scientific and Biomedical Research	1
A Simple and Economical Site-Directed Mutagenesis Method for Large Plasmids by Direct Transformation of two Overlapping PCR Fragments	1
Removal of Nonspecific Binding Proteins is Required in Co-Immunoprecipitation with Nuclear Proteins	1
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	1
A novel device for buffy coat collection	1
Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate	1
Cytotoxic T Lymphocytes Targeting a Conserved SARS-CoV-2 Spike Epitope are Efficient Serial Killers	1
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	1
Advances of Multiplex Ligation-dependent Probe Amplification Technology in Molecular Diagnostics	1
An enzyme-free alcohol-based organoid harvesting solution	1
Methodology Established for the Detection of Circulating Tumor DNA by Hybridization Capture	1
A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research	1
Efficient production system for hydrogel-based transparent soil for plant root observation	1
MSCProfiler: A Single Cell Image Processing Workflow to Investigate Mesenchymal Stem Cell Heterogeneity	1
A Simple and Rapid Method for Isolating High-quality RNA from Kenaf with High Polysaccharide and Polyphenol Contents	1
Corrigendum	1
Flow Cytometry: Past and Future	1
Comparison of Methods for Mapping Rhizosphere Processes in the Context of Their Surrounding Root and Soil Environments	1
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	1
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	1