Biotechniques

Article	Citations
Recombinant Bacterial Endotoxin Testing: A Proven Solution	62
UK Biobank: what can it do, how you can use it and how is it being used?	55
Development of a Droplet Digital™ PCR DNA Methylation Detection and Quantification Assay of Prenatal Tobacco Exposure	46
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations	40
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones	40
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations	24
Rectal swab DNA collection protocol for PCR genotyping in rats	23
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment	19
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system	19
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots	18
Correction	16
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development	14
Welcome to the 72nd Volume of BioTechniques	13
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide	13
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation	12
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR	12
Comparison of DNA Extraction Methods for Samples from Old Blood Collections	11
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study	11
Education in Microscopy: Taking a Closer Look with Jennifer Waters	10
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations	10
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue	10
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach	10
Automated Epidermal Thickness Quantification of in Vitro Human Skin Equivalents Using Optical Coherence Tomography	9
The Forensic Genomics Toolbox is Expanding	9
Selective Expansion of Target Cells Using the Enrich TROVO Platform	9

Secure and Optimized Detection of PNPLA3 rs738409 Genotype by an Improved PCR–Restriction Fragment Length Polymorphism Method	9
Gel Purification of gDNA for Next-Generation Sequencing Applications	9
How has CRISPR Transformed Therapeutic Drug Discovery?	9
Who wants to live forever? Models shaping the future of aging research	7
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds	7
Techniques to Save the Sea	7
Tergitol-15-S-9 Inactivates SARS-CoV-2 and Boosts Immunoassay Signals	7
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology	7
Improved SARS-CoV-2 PCR Detection and Genotyping with Double-Bubble Primers	7
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells	7
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data	7
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR	6
High Transfection Efficiency and Cell Viability of Immune Cells with Nanomaterials-Based Transfection Reagent	6
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )	6
When is an SNP not an SNP?	6
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples	6
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection	6
Yielding Quality Viral RNA by Using Two Different Chemistries: A Comparative Performance Study	6
Corrigendum	6
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols	5
Design and Characterization of a 3D-printed Staggered Herringbone Mixer	5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?	5
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels	5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics	5
Ladder-Shape Melting Temperature Isothermal Amplification of Nucleic Acids	5
Extraterrestrial agriculture: plant cultivation in space	5
Urgency of Rapid Legionella Detection Post-COVID-19 Lockdowns: an Interview with Elizabeth Minogue	5
A Simplified Method for Bronchoalveolar Lavage in Mice by Orotracheal Intubation Avoiding Tracheotomy	5
Intracellular Flow Cytometry Complements RT-qPCR Detection of Circulating SARS-CoV-2 Variants of Concern	5
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement	4
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA	4
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion	4
Adenovirus Vector System: Construction, History and Therapeutic Applications	4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction	4
Bioinformaticians: the Hidden Heroes of the COVID-19 Pandemic	4
Simple, Inexpensive and RNase-Free Purification of Plasmid DNA by Fractional Precipitation with Isopropanol	4
X Marks the Spot: Mapping the Brain with EEG and MRI	4
Smart PCR Machines Can Reduce the Risk of Carryover Contamination	4
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.	4
Apoptosis Assessment in High-Content and High-Throughput Screening Assays	4
Democratizing Bioinformatics through easily Accessible Software Platforms for Non-Experts in the Field	4
Colonoscopy-Based Intramucosal Transplantation of Cancer Cells for Mouse Modeling of Colon Cancer and Lung Metastasis	4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates	4
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis	4
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests	4
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues	4
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography	4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education	4
Who Owns Your DNA?	4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling	4

Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel	3
Premature Termination Codon: A Tunable Protein Translation Approach	3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle	3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection	3
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis	3
A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase	3
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay	3
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital	3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli	3
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies	3
Development of an enhanced anti-pan-N-formylmethionine-specific antibody	3
Corrigendum	3
Psychobiotics: Are we on the Cusp of a Breakthrough?	3
A drug screening platform for protein expression levels in neurological disorders	3
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization	3
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems	3
Microbial Forensics: What We've Learned from Amerithrax and Beyond	3
CRISPR-PN2: A Flexible and Genome-Aware Platform for Diverse CRISPR Experiments in Parasitic Nematodes	3
A Fast, Nondestructive Method for the Detection of Disease-Related Lesions and Wounded Leaves	3
Decoding the Mechanisms of Embryo Development with Spatial Biology	3
Cancer Vaccinations: A Personalized Approach	3
Single-Cell RNA Sequencing of Intramedullary Canal Tissue to Improve Methods for Studying Fracture Repair Biology	2
Sampling and analysis methods of air-borne microorganisms in hospital air: a review	2
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports	2
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE	2
Effects of a Fully Enclosed Hollow-Fiber Centrifugal Ultrafiltration Technique for Laboratory Biosafety Improvement	2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples	2
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing	2
Toxoplasma Gondii : The Kitty Litter Parasite	2
Fast Assay to Predict Multipotent Mesenchymal Stromal Cell Replicative Senescence Dynamics	2
Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications	2
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes	2
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution	2
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile	2
Novel buffer for long-term preservation of DNA in biological material at room temperature	2
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues	2
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?	2
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein	2
A Simplified Procedure for Isolation of Primary Murine Microglia	2
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis	2
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information	2
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)	2
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics	2
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome	2
A Simple Approach for Effective Shearing and Reliable Concentration Measurement of Ultra-High-Molecular-Weight DNA	2
Adaptation of the HEp-2 Cell Line to toTally Animal-Free Culture Systems and Real-Time Analysis of Cell Growth	2
A Compact and Simple Method of Achieving Differential Transgene Expression by Exploiting Translational Readthrough	1
Ancient DNA Sequencing: Telling the Tale of Human History and Evolution	1
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events	1
Optimized RNA Isolation of FFPE Uterine Scar Tissues for RNA Expression Analyses Delineated by Laser Microdissection	1
Effective Strategies for Isolating DNA from Members of Asteraceae with High Concentrations of Secondary Metabolites	1
Cradle Cultures: Growing Stem Cell-Derived Developmental Cell Models in Vitro	1
Instant Pot for Antigen Retrieval: A Simple, Safe and Economical Method for Use in Immunohistochemistry	1
A Simple and Rapid Method for Isolating High-quality RNA from Kenaf with High Polysaccharide and Polyphenol Contents	1
High-Yield Expression of Periplasmic Single-Chain Variable Fragments by Solid Escherichia Coli Cultures	1
Endogenous Cell-free DNA in Fetal Bovine Serum Introduces Artifacts to in Vitro cell-free DNA Models	1
An Optimal Genomic Dna Extraction Method for Shoots of Four Dendrocalamus Species Based on Membership Function Analysis	1
Wheat Improvement Using Genome Editing Technology	1
Legionella Water Testing and the EU Drinking Water Directive: Could Potentially Harmful Legionella Bacteria Slip Through the Gaps?	1
HIV: The Pursuit of an Elusive Vaccine	1
Golden Gate Assembly of BioBrick-Compliant Parts Using Type II Restriction Endonucleases	1
A Simplified and Rapid in Situ Hybridization Protocol for Planarians	1
Flow Cytometry: Past and Future	1
The Path to Solving the Protein Folding Problem	1
Adipocyte ABCA1 expression analysis using flow cytometry	1
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry	1
The Determination of Cystatin C in Biological Samples via the Surface Plasmon Resonance Method	1
Special Focus Issue - Celebrating 40 Years of Publishing Lab Techniques: Looking Back to Look Foreword	1
Easy Method for Six-Fragment Golden Gate Assembly of Modular Vectors	1
A Building Block-Based Beam-Break (B 5 ) Locomotor Activity Monitoring System and Its Use in Circadian Biology Research	1
How is Spatial Transcriptomics Influencing Cancer Research and Diagnostics?	1
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol	1
Metagenomic Nanopore Sequencing Versus Conventional Diagnosis for Identification of the Dieback Pathogens of Mango Trees	1
Comparison of Optimized Methodologies for Isolating Nuclei from Esophageal Tissue	1
Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia Pastoris	1
Extracting High-Quality RNA From Formaldehyde-Fixed Naturally Aged Neuromusculoskeletal Tissues	1
Colorimetric Analysis of C-reactive Protein Via ‘Jellyfish’ Probe-based Exonuclease III-assisted Multiple-Signal Recycles	1
Artificial intelligence: help or hindrance in solving the reproducibility crisis?	1
Rapid Microfluidic Isolation of Virally Infected Primary Bronchial Epithelial Cells for Single-Cell RNA Sequencing	1
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions	1

Non-Destructive Extraction of DNA from Preserved Tissues in Medical Collections	1
EXtraction of Cucumber Phloem Sap Based on the Capillary–Air Pressure Principle	1
Development of a human tyrosinase activity inhibition assay using human melanoma cell lysate	1
Mapping the spread of antibiotic resistance genes in the coastal microbiome	1
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection	1
Generation of UL128 -shRNA Transduced Fibroblasts for the Release Of Cell-free Virus from Clinical Human Cytomegalovirus Isolates	1
Sample Collection/Stabilization and DNA/RNA Extraction from Swab Samples for Microbiome or Metagenome Analyses	1
Methodology Established for the Detection of Circulating Tumor DNA by Hybridization Capture	1
Tumor Cell Invasion into Matrigel: Optimized Protocol for RNA Extraction	1
Advances of Multiplex Ligation-dependent Probe Amplification Technology in Molecular Diagnostics	1
A Comparison of Six DNA Extraction Protocols for 16S, ITS and Shotgun Metagenomic Sequencing of Microbial Communities	1
The Fluorescent Protein Stability Assay: An Efficient Method For Monitoring Intracellular Protein Stability	1
Lab Techniques for a More Sustainable World	1
Considerations for Colorblind Individuals on Selecting Colorimetric or Fluorescent Dye Assay Outcomes	1
Functionalized primer initiated signal cycles and personal glucose meter for sensitive and portable miRNA analysis	1
Polyacrylamide Gel Electrophoresis: A Versatile Tool for the Separation of Nanoclusters	1
The Earth BioGenome Project: A Success Story for Whole-Genome Sequencing?	1
Efficient production system for hydrogel-based transparent soil for plant root observation	1
Sociometric Wearable Devices for Studying Human Behavior in Corporate and Healthcare Workplaces	1
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?	1
Comparison of Methods for Mapping Rhizosphere Processes in the Context of Their Surrounding Root and Soil Environments	1
DNA Extraction from Clotted Blood in Genotyping Quality	1
Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses	1
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma	1