Biotechniques

Papers
(The median citation count of Biotechniques is 2. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-05-01 to 2026-05-01.)
ArticleCitations
Site-Specific Analysis of Ribosomal 2′O-Methylation by Quantitative Reverse Transcription PCR Under Low Deoxynucleotide Triphosphate Concentrations140
UK Biobank: what can it do, how you can use it and how is it being used?73
Ancient DNA Analysis from Epoxy Resin Biodur ® -embedded bones71
Decoding Disease: Scott Patterson’s Perspectives on the Power of Biomarkers in Drug Development59
pJoseph2: a family of plasmids as positive controls for bacterial protein expression, transfections, and western blots25
Rectal swab DNA collection protocol for PCR genotyping in rats24
Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system20
Correction18
Comparison of commercially available DNA and RNA extraction kits for wildlife feces collected from the environment16
‘Gel-Stacks’ gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study16
Prioritizing privacy and presentation of supportable hypothesis testing in forensic genetic genealogy investigations16
Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach14
Peter and the Beanstalk: Tackling the Giant Questions of Soybean Nodulation14
An Industry Perspective Approach and Control Strategy for Implementation of Ready-to-Use Cells in Bioassays: Survey Outcome And Recommendations14
Education in Microscopy: Taking a Closer Look with Jennifer Waters14
Gibson Assembly Interposition Improves Amplification Efficiency of Long DNA and Multifragment Overlap Extension PCR14
Three-Dimensional Visualization and Analysis of Dendritic Spines in Human Brain Tissue12
Who wants to live forever? Models shaping the future of aging research9
Comparison of Colorimetric, Spectroscopic and Electrochemical Techniques for Quantification of Hydrogen Sulfide9
Selective Expansion of Target Cells Using the Enrich TROVO Platform9
Simple, Fast and Inexpensive Hot Sodium Hydroxide and Tris DNA Extraction Method for Genotyping Tomato and Melon Seeds9
How has CRISPR Transformed Therapeutic Drug Discovery?9
Integration of mass cytometry and single-cell RNA-sequencing of cells in bronchoalveolar lavage8
Direct-Current Electric Field Stimulation Promotes Proliferation and Maintains Stemness of Mesenchymal Stem Cells8
When is an SNP not an SNP?7
Accurate Prediction of Serum Antibody Levels from Noninvasive Saliva/Nasal Samples7
Alkaline dip DNA extraction from skin mucus for high-throughput sexing of sterlets ( Acipenser ruthenus )7
Gel Purification of gDNA for Next-Generation Sequencing Applications7
Cancer’s Sweet Spot: Techniques to Harness Saccharides in Tumor Biology6
Welcome to the 74th Issue of BioTechniques : 40 years of Publishing the Latest Laboratory Protocols6
Reference Genes for Gene Expression Profiling in Mouse Models of Listeria Monocytogenes Infection6
A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA6
qDATA - an R application implementing a practical framework for analyzing quantitative real-time PCR data6
Evaluation and Validation of reference Genes in Cymbidium Faberi for real-time Quantitative PCR6
A Simple Drying Solution that Minimizes Cracking During Air-Drying of Polyacrylamide Gels6
Methods to Evaluate the Impact of SARS-CoV-2 Nucleocapsid Mutations on Antigen Detection by Rapid Diagnostic Tests5
X Marks the Spot: Mapping the Brain with EEG and MRI5
Correction5
Age is Just a Number: Can Proteomic Analysis Provide Insight into the Aging Body?5
PCR-Based Gene Synthesis with Overlapping Unisense-Oligomers Asymmetric Extension Supported by a Simulator for Oligonucleotide Extension Achieved 1 kbp dsDNA5
Modification of Grocott's Staining Procedure with Heat Treatment and Oxidation by Periodic Acid for Mucormycosis in Tissue: A Method to Detect Mucor spp.5
Corrigendum5
SUMOylation of the Chromodomain Factor MRG-1 in C. Elegans Affects Chromatin-Regulatory Dynamics5
The dynamic duo: cryo-EM teams up with machine learning to visualize biomolecules in motion5
A Cryostat-Based Frozen Section Method to Increase the Yield of Extracellular Vesicles Extracted from Different Tissues5
Extraterrestrial agriculture: plant cultivation in space5
Self-circularized Overhang-based Unrecombined Products (SOUP): a rapid in vitro method for generating backbone-free expression cassettes5
Freezing diluted bovine serum albumin standards does not significantly affect standard curves5
Determination of n-3 Index and Arachidonic Acid/eicosapentaenoic Acid Ratio in Dried Blood Spot by Gas Chromatography5
Biohacking the Food Chain: Using CRISPR to Combat the Global Food Crisis4
Modular probe-based colorimetric miRNA detection via polymerase/endonuclease assisted chain displacement4
Decoding the Mechanisms of Embryo Development with Spatial Biology4
A simple and rapid in vitro assay for identification of direct inhibitors of O 6 -methylguanine-DNA methyltransferase4
Adenovirus Vector System: Construction, History and Therapeutic Applications4
Importance of a reference ladder in analytical testing of CRISPR HDR donor ssDNA templates4
Developing Virtual and Augmented Reality Applications for Science, Technology, Engineering and Math Education4
PS4: A Next-Generation Dataset for Protein Single-Sequence Secondary Structure Prediction4
Releasing Secrets Bound to Ancient Remains with Modern DNA Extraction Techniques: An Interview with Elena Essel4
Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization4
3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders4
Label-free DNA-sensor modelling based on magnetic induction spectroscopy4
Influence of Resuspension Volume on Dry Sampling Devices Taken for Human Papillomavirus Testing: Implications for Self-Sampling4
Assessment of Measurement Accuracy of Amplified Dna Using a Colorimetric Loop-Mediated Isothermal Amplification Assay4
A drug screening platform for protein expression levels in neurological disorders4
Chromatin Immunoprecipitation and Library Preparation: a Powerful Tool to Unravel the Epigenome3
A Simplified Procedure for Isolation of Primary Murine Microglia3
Toxoplasma Gondii : The Kitty Litter Parasite3
Development and evaluation of a single-tube nested PCR with colorimetric assay for Mycobacterium tuberculosis detection3
RNA extraction and RNA-sequencing method for transcriptomic analysis of Mycobacterium tuberculosis3
Engineering an Incubation Environment that Mimics in situ Conditions for in vitro coastal microbiome studies3
Utilizing the biosimulator to analyze the environmental microbiome within the intensive care units of a hospital3
Optimization of agarose–gelatin double embedding to minimize sectioning artifacts in multicellular spheroids3
Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information3
Spatial proteomics in the age of artificial intelligence: exploring the impact on cancer research3
Long-Read Sequencing for the Metagenomic Analysis of Microbiomes3
Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution3
A Simple, Sensitive and Colorimetric Assay for Pseudomonas Aeruginosa Infection Analysis3
Premature Termination Codon: A Tunable Protein Translation Approach3
Specific N-Terminal Amino Acids Potentiate the Periplasmic Expression of Single-Chain Variable Fragments in Escherichia Coli3
Sampling and analysis methods of air-borne microorganisms in hospital air: a review3
Analytical validation of the IBD segment-based tool KinSNP ® for human identification applications3
Development of an enhanced anti-pan-N-formylmethionine-specific antibody3
Application of a 3D Bioprinter: Jet Technology For ‘Biopatch’ Development Using Cells on Hydrogel Supports3
Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile3
Nanotechnology and Cancer Therapeutics: Delivering on the Hype?3
Evaluation Protocol for CRISPR/Cas9-mediated CD19 Knockout GM24385 Cells by Flow Cytometry and Sanger Sequencing3
Preparation of Bacillus Subtilis Cell Samples and Generation of an SDS-PAGE3
Dual-mode detection of glucose based on pistol-like DNAzyme-mediated exonuclease-assisted signal cycle3
Western Blotting (immunoblotting): History, Theory, Uses, Protocol and Problems3
A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein3
Microbial Forensics: What We've Learned from Amerithrax and Beyond3
Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP)3
Benchmarking antibody discovery fidelity and reproducibility with an assay-locked residue fidelity index3
Prediction of High Fecal Propionate-to-Butyrate Ratios Using 16S rRNA-Based Detection of Bacterial Groups with Liquid Array Diagnostics2
Stabilization of human RNA and DNA in stool samples at room temperature2
Mito-Kaede photoactivation and chase experiment for mitophagy: mitophagy flux response toward various stimulations2
Cradle Cultures: Growing Stem Cell-Derived Developmental Cell Models in Vitro2
A Simplified and Rapid in Situ Hybridization Protocol for Planarians2
HIV: The Pursuit of an Elusive Vaccine2
Taking root: the techniques growing genetically engineered plants2
Quantitative Analysis of lncRNA in Formalin-fixed Paraffin-embedded Tissues of Oral Squamous Cell Carcinoma2
Quantitative analysis of oligonucleotide delivery into isolated mitochondria: a proof-of-concept method2
A clinical biomarker assay to quantitate CXCL9 in human serum2
Amberlite XAD-4 is a Convenient Tool for Removing Triton X-100 and Sarkosyl from Protein Solutions2
DNA Extraction from Clotted Blood in Genotyping Quality2
Efficient production system for hydrogel-based transparent soil for plant root observation2
The Path to Solving the Protein Folding Problem2
Artificial intelligence: help or hindrance in solving the reproducibility crisis?2
I Know What You're Thinking; Can Neuroimaging Truly Reveal Our Innermost Thoughts?2
Mapping the spread of antibiotic resistance genes in the coastal microbiome2
Pre-sequencing assessment of RNA-Seq library quality using real-time qPCR2
Development of a Highly Sensitive Nucleic Acid Amplification-Based Detection for Human Leptospirosis Infection2
Streamlining single-cell spatial transcriptomics for human kidney tissue2
Parameter optimization of NanoJ-SRRF for live-cell microtubule imaging2
Impact of commercial RNA extraction methods on the recovery of human RNA sequence data from archival fixed tissues2
Considerations for Colorblind Individuals on Selecting Colorimetric or Fluorescent Dye Assay Outcomes2
AxioParse: streamlining Axiom Microbiome assay data processing and dataset generation2
A Simple and Rapid Method for Isolating High-quality RNA from Kenaf with High Polysaccharide and Polyphenol Contents2
Single-cell RNA Sequencing of Bronchoscopy Specimens: Development of a Rapid Minimal Handling Protocol2
Comparison of purification protocols for effective large-primer removal between rounds of PCR amplification2
Target-Seq: Single Workflow for Detection of Genome Integration Site, DNA Translocation and Off-Target Events2
Isolation and profiling of single circulating tumor cells in myeloma: a new workflow for liquid biopsies2
Impact of ambient temperature exposure on miRNA stability in human plasma2
A Technique for the Masses: Single-Cell Analysis Using Mass Cytometry2
Novel buffer for long-term preservation of DNA in biological material at room temperature2
Comparison of Magnetic Bead and Rapid Swab RNA Extraction Methods for Detecting Rabbit Hemorrhagic Disease Virus 2 in Rabbit Liver Samples2
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