OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Advances is 28. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-02-01 to 2025-02-01.)

Article	Citations
Ionic liquids and deep eutectic solvents for the stabilization of biopharmaceuticals: A review	418
An integrated perspective on measuring cytokines to inform CAR-T bioprocessing	382
Use of additives to improve collective biogas plant performances: A comprehensive review	176
Computational design of enzymes for biotechnological applications	169
Employment of the CRISPR/Cas9 system to improve cellulase production in Trichoderma reesei	169
Collagen processing with mesoscale aggregates as templates and building blocks	161
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	141
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	140
Nucleic acid-responsive smart systems for controlled cargo delivery	137
Current status, challenges and prospects for lignin valorization by using Rhodococcus sp.	137
Microbial lifelines in bioprocesses: From concept to application	120
Production and characterization of starch-lignin based materials: A review	116
Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration	113
Non-viral engineering of NK cells	107
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	107
Editorial Board	107
Engineering the next-generation synthetic cell factory driven by protein engineering	106
Developments and prospects for doubled haploid wheat	101
Microbial cell surface engineering for high-level synthesis of bio-products	96
Editorial Board	95
Functional expansion of the natural inorganic phosphorus starvation response system in Escherichia coli	94
Editorial Board	93
Editorial Board	90
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	89
Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms	86

Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	83
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	80
Identification, characterization, and engineering of glycosylation in thrombolytics	76
Insights into Clostridium tetani: From genome to bioreactors	75
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	75
Biofilm control by interfering with c-di-GMP metabolism and signaling	73
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	72
Engineering status of protein for improving microbial cell factories	72
Biophotovoltaics: Recent advances and perspectives	69
Functional roles and engineering strategies to improve the industrial functionalities of lactic acid bacteria during food fermentation	68
Recent advances in the improvement of bi-directional electron transfer between abiotic/biotic interfaces in electron-assisted biosynthesis system	67
New trends in biotechnological applications of photosynthetic microorganisms	67
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	65
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	65
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	65
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	63
Antimicrobial properties and applications of metal nanoparticles biosynthesized by green methods	61
Insights on the emerging biotechnology of histidine-rich peptides	60
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	60
Recent advancements and challenges in emerging applications of biochar-based catalysts	59
Bacterial volatile organic compounds as biopesticides, growth promoters and plant-defense elicitors: Current understanding and future scope	59
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	59
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	59
Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects	58
3D biofabrication and space: A ‘far-fetched dream’ or a ‘forthcoming reality’?	58
New perspectives into Gluconobacter-catalysed biotransformations	55
New opportunities and perspectives on biosynthesis and bioactivities of secondary metabolites from Aloe vera	55
Bioprospecting for bioactive compounds in microalgae: Antimicrobial compounds	55
Manufacturing of bacteriophages for therapeutic applications	55
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	54
Recent developments in animal venom peptide nanotherapeutics with improved selectivity for cancer cells	54
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	54
Recent advances in fed-batch microscale bioreactor design	53
Library and post-translational modifications of peptide-based display systems	53
Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications	52
Kluyveromyces marxianus as a microbial cell factory for lignocellulosic biomass valorisation	51
Using nanomaterials to increase the efficiency of chemical production in microbial cell factories: A comprehensive review	51
Trait drift in microalgae and applications for strain improvement	51
Recent advances in human respiratory epithelium models for drug discovery	51
The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic	50
The molecular basis and enzyme engineering strategies for improvement of coupling efficiency in cytochrome P450s	49
IgG glycans in health and disease: Prediction, intervention, prognosis, and therapy	49
Bacillus licheniformis: The unexplored alternative for the anaerobic production of lipopeptide biosurfactants?	49
Biological production of medium-chain carboxylates through chain elongation: An overview	48
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	48
Biosensor-guided discovery and engineering of metabolic enzymes	47
Recent advances in biocatalysis of nitrogen-containing heterocycles	47
Role of distal sites in enzyme engineering	47
Computer-aided understanding and engineering of enzymatic selectivity	47
Embracing a low-carbon future by the production and marketing of C1 gas protein	47

Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	46
Contribution of quorum sensing to virulence and antibiotic resistance in zoonotic bacteria	46
Carbohydrate flow through agricultural ecosystems: Implications for synthesis and microbial conversion of carbohydrates	46
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	45
Comprehensive utilization of sucrose resources via chemical and biotechnological processes: A review	45
Native, engineered and de novo designed ligands targeting the SARS-CoV-2 spike protein	45
Flavoprotein monooxygenases: Versatile biocatalysts	45
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	44
Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices	44
Next generation probiotics: Engineering live biotherapeutics	43
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	42
Editorial Board	42
Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis	42
Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria	42
Recent advances in culture medium design for enhanced production of monoclonal antibodies in CHO cells: A comparative study of machine learning and systems biology approaches	41
Leveraging independent component analysis to unravel transcriptional regulatory networks: A critical review and future directions	40
Advances and challenges in preparing membrane proteins for native mass spectrometry	40
Pathway to industrial application of heterotrophic organisms in critical metals recycling from e-waste	40
Microbial carbohydrate-binding toxins – From etiology to biotechnological application	39
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	39
Metabolic engineering of Corynebacterium glutamicum: Unlocking its potential as a key cell factory platform for organic acid production	39
Advances in enzymatic oxyfunctionalization of aliphatic compounds	39
Rare rhizo-Actinomycetes: A new source of agroactive metabolites	39
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	39
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	39
ATP regeneration by ATPases for in vitro biotransformation	38
Endogenous CRISPR-Cas mediated in situ genome editing: State-of-the-art and the road ahead for engineering prokaryotes	38
Factors affecting the quality of therapeutic proteins in recombinant Chinese hamster ovary cell culture	38
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	38
Application of microbial 3-ketosteroid Δ1-dehydrogenases in biotechnology	37
Strategies for the biological production of ectoine by using different chassis strains	37
Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications	37
Rugose small colony variant and its hyper-biofilm in Pseudomonas aeruginosa: Adaption, evolution, and biotechnological potential	37
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	37
Digital models in biotechnology: Towards multi-scale integration and implementation	37
Carbohydrate binding modules: Compact yet potent accessories in the specific substrate binding and performance evolution of carbohydrate-active enzymes	36
Recent advances in phyto-combined remediation of heavy metal pollution in soil	36
Design and application of microfluidics in aptamer SELEX and Aptasensors	35
Advances in biosynthesis and downstream processing of diols	35
Bacillus sp. as a microbial cell factory: Advancements and future prospects	35
An overview of biomethanation and the use of membrane technologies as a candidate to overcome H2 mass transfer limitations	35
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	35
The role of orphan crops in the transition to nutritional quality-oriented crop improvement	34
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	34
Engineering conditional protein-protein interactions for dynamic cellular control	34
Cell wall polysaccharides of streptococci: A genetic and structural perspective	34
Protein representations: Encoding biological information for machine learning in biocatalysis	34
Expanding the horizon of transient CAR T therapeutics using virus-free technology	34
New trends in industrial biocatalysis	33
Editorial Board	33
Editorial Board	33
The thin line between monooxygenases and peroxygenases. P450s, UPOs, MMOs, and LPMOs: A brick to bridge fields of expertise	32
Toward biomanufacturing of next-generation bacterial nanocellulose (BNC)-based materials with tailored properties: A review on genetic engineering approaches	32
Biobased short chain fatty acid production - Exploring microbial community dynamics and metabolic networks through kinetic and microbial modeling approaches	32
Editorial Board	32
Folding of heterologous proteins in bacterial cell factories: Cellular mechanisms and engineering strategies	32
Glycosylation shapes the efficacy and safety of diverse protein, gene and cell therapies	31
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	31
Stimulated Raman scattering microscopy in chemistry and life science – Development, innovation, perspectives	31
Emerging biotechnologies for evaluating disruption of stress, sleep, and circadian rhythm mechanism using aptamer-based detection of salivary biomarkers	31
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	31
The essential role of mRNA degradation in understanding and engineering E. coli metabolism	31
A journey with psychedelic mushrooms: From historical relevance to biology, cultivation, medicinal uses, biotechnology, and beyond	31
Towards engineered yeast as production platform for capsaicinoids	31
Recent advances in molecular farming using monocot plants	30
The new frontier in CHO cell line development: From random to targeted transgene integration technologies	30
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	30
Challenges and progress towards industrial recombinant protein production in yeasts: A review	30
Genome edited wheat- current advances for the second green revolution	29
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	29
Development and applications of mRNA treatment based on lipid nanoparticles	29
Biopreservation of beer: Potential and constraints	29
Recent advances in design and application of synthetic membraneless organelles	29
Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts	28
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	28
Engineering microbial metabolic energy homeostasis for improved bioproduction	28
From bumblebee to bioeconomy: Recent developments and perspectives for sophorolipid biosynthesis	28
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	28
Overview of indigo biosynthesis by Flavin-containing Monooxygenases: History, industrialization challenges, and strategies	28
Improving microalgae for biotechnology — From genetics to synthetic biology – Moving forward but not there yet	28