Biotechnology Advances

Article	Citations
Editorial Board	498
Use of additives to improve collective biogas plant performances: A comprehensive review	427
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	212
Collagen processing with mesoscale aggregates as templates and building blocks	200
Recent advances in human respiratory epithelium models for drug discovery	178
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	176
Microbial cell surface engineering for high-level synthesis of bio-products	175
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	174
New perspectives into Gluconobacter-catalysed biotransformations	154
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	151
Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects	143
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	139
Computer-aided understanding and engineering of enzymatic selectivity	127
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	125
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	119
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	117
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	115
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	114
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	112
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	112
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	111
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	109
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	102
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	96
Proteomic and Glyco(proteo)mic tools in the profiling of cardiac progenitors and pluripotent stem cell derived cardiomyocytes: Accelerating translation into therapy	95

Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	92
Microbial starch debranching enzymes: Developments and applications	90
Biological valorization of lignin to flavonoids	89
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	85
Growth-coupled high throughput selection for directed enzyme evolution	85
Recent advances in machine learning applications in metabolic engineering	84
Editorial Board	82
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	81
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	80
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	80
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	80
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	78
Biotechnological applications of purine and pyrimidine deaminases	78
Using machine learning approaches for multi-omics data analysis: A review	77
Pseudomonas aeruginosa biofilms and their partners in crime	76
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	76
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	76
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	75
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	75
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	74
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	72
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	72
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	70
Rutinosidase and other diglycosidases: Rising stars in biotechnology	70
Recent advances in research for potential utilization of unexplored lichen metabolites	70
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	68
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	68
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	66
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	66
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	65
Stress response in Rhodococcus strains	64
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	64
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	64
Resource allocation in mammalian systems	64
Research progress of engineering microbial cell factories for pigment production	63
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	63
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	63
Editorial Board	62
Objectives, benefits and challenges of bioreactor systems for the clinical-scale expansion of T lymphocyte cells	60
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	59
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	59
Recent advances in lipid metabolic engineering of oleaginous yeasts	59
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	59
Detection, production, modification, and application of arylsulfatases	59
Recent advances in enzyme-enhanced immunosensors	59
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	58
Engineering membrane architecture for biotechnological applications	58
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	58
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	58
Recent advances in engineering synthetic biomolecular condensates	58

Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	58
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	57
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	57
Interpretable and explainable predictive machine learning models for data-driven protein engineering	57
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	55
Editorial Board	55
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	55
Editorial Board	55
Biotechnological applications of type 1 secretion systems	54
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	53
A guide to the use of bioassays in exploration of natural resources	53
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	52
Optimizing microbial networks through metabolic bypasses	52
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	51
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	51
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	51
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	51
Advance computational tools for multiomics data learning	50
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	50
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	49
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	49
Principles of antibodies with ultralong complementarity-determining regions and picobodies	48
Bacterial cellulose and its potential for biomedical applications	48
The technology, opportunities, and challenges of Synthetic Biological Intelligence	48
Functionalized chitosan for cancer nano drug delivery	48
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	47
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	47
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	47
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	47
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	47
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	46
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	46
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	46
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	46
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	46
Biofilm control by interfering with c-di-GMP metabolism and signaling	46
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	46
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	46
Strategies for the biological production of ectoine by using different chassis strains	46
Trait drift in microalgae and applications for strain improvement	46
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	46
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	45
Challenges and progress towards industrial recombinant protein production in yeasts: A review	45
Optogenetic technologies in translational cancer research	45
Biopreservation of beer: Potential and constraints	45
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	44
Protein representations: Encoding biological information for machine learning in biocatalysis	44
Design and application of microfluidics in aptamer SELEX and Aptasensors	44
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	44
Microbial gas fermentation technology for sustainable food protein production	44
Plants arabinogalactans: From structures to physico-chemical and biological properties	44
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	43
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	42
Recent advances in phyto-combined remediation of heavy metal pollution in soil	42
The isolation of lignin with native-like structure	42
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	42
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	42
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	40
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	40
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	40
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	40
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	40
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	39
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	38
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	38
Applications of bio-capacitance to cell culture manufacturing	38
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	38
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	38
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	37
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	36
The emerging role of cellular post-translational modifications in modulating growth and productivity of recombinant Chinese hamster ovary cells	36
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	36
Computational advances in biosynthetic gene cluster discovery and prediction	36
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	36
From natural to synthetic: Promoter engineering in yeast expression systems	36
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	36
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	35
In vitro models of gut digestion across childhood: current developments, challenges and future trends	35
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	35
Refactoring transcription factors for metabolic engineering	35

Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	34
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	34
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	34
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	34
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	34