Biotechnology Advances

Article	Citations
Editorial Board	347
Editorial Board	198
Targeted analysis of metagenomes: divide and conquer	178
Collagen processing with mesoscale aggregates as templates and building blocks	172
Methanol metabolism in synthetic methylotrophic microorganisms	170
New perspectives into Gluconobacter-catalysed biotransformations	165
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	164
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	153
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	147
Use of additives to improve collective biogas plant performances: A comprehensive review	137
Biotechnological production and emerging applications of betalains: A review	132
Biotechnological advances in key regulatory genes of phenylpropanoid and terpenoid biosynthesis pathways in Panax ginseng: Current insights and future prospects	130
Decoding microbial interactions: Interaction networks and regulatory strategies for medium-chain fatty acid biosynthesis through anaerobic chain elongation	129
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	127
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	125
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	124
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	124
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	124
Benzylisoquinoline alkaloid production: Moving from crop farming to chemical and biosynthesis	123
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	112
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	112
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	111
Microbial computing: Review and Perspectives	109
Machine learning methods for small data and upstream bioprocessing applications: A comprehensive review	107
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	105

Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	103
Editorial Board	99
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	99
Recent advances in machine learning applications in metabolic engineering	98
Growth-coupled high throughput selection for directed enzyme evolution	97
Biological valorization of lignin to flavonoids	95
Emerging research insights and future perspectives on the advancement of eccDNA: A comprehensive review	92
The path to biotechnological singularity: Current breakthroughs and outlook	92
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	90
Advances and challenges in enzymatic rubber degradation: Exploring genetic, molecular, and biotechnological aspects	89
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	89
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	87
Advances in gene editing-led route for hybrid breeding in crops	85
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	84
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	83
Plant cell wall biosynthesis: Immune signaling, genome editing, and physiological implications for biomass valorization	83
Advances in transcription factor-based biosensors for natural product biosynthesis: Optimization, emerging technologies, and future prospects	80
Droplet microfluidics for single-cell analysis: From improved cell encapsulation and sorting technologies to innovative applications	79
Computational methods for spatial multi-omics integration	79
Protease engineering: Approaches, tools, and emerging trends	78
Haloferax and the Haloferacaceae: Potential role in bioindustry	78
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	78
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	78
Biotechnological applications of purine and pyrimidine deaminases	77
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	77
Polyethylene biodegradation: A multifaceted approach	77
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	76
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	76
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	76
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	76
Editorial Board	76
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	76
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	76
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	74
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	73
Optimizing prime editing: Advances in efficiency enhancement	72
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	71
Rutinosidase and other diglycosidases: Rising stars in biotechnology	71
Resource allocation in mammalian systems	70
Loop engineering in enzymes from structure to function: Mechanisms, methodologies, and engineering strategies	70
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	70
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	69
The fungal cure: Harnessing mycelial approach as sustainable green solution for industrial waste treatment	67
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	67
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	66
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	66
Research progress of engineering microbial cell factories for pigment production	66
Recent advances in research for potential utilization of unexplored lichen metabolites	65
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	64
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	64

Editorial Board	63
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	62
Construction and applications of methanol bio-converting cell factories	62
UGT708 glycosyltransferases: Nature's architects of C-glycosides	62
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	61
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	61
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	59
Microalgal bioengineering for futuristic applications in synthetic and space biology	59
Detection, production, modification, and application of arylsulfatases	59
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	58
Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	58
Machine learning in predictive biocatalysis: A comparative review of methods and applications	57
Harnessing the biomolecular mechanisms of marine biomineralisation for carbon sequestration	56
Recent advances in engineering synthetic biomolecular condensates	56
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	56
Aptamer screening within sample matrices: Toward reliable applications	54
Principles of antibodies with ultralong complementarity-determining regions and picobodies	54
Biotechnology-enabled plastic valorization across enzymatic, microbial, and hybrid conversion systems: A review	54
Engineering membrane architecture for biotechnological applications	54
Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications	54
Flavin reductases in two-component systems: Mechanistic insights, structural classification, and biotechnological advances	53
Editorial Board	53
Microbial collaboration in bioelectrochemical systems: Mechanisms and advances towards sustainable biobased products	53
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	53
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	53
Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects	52
Enzyme miniaturization: Revolutionizing future biocatalysts	52
Optimizing microbial networks through metabolic bypasses	52
Smart culture medium optimization for recombinant protein production: Experimental, modeling, and AI/ML-driven strategies	50
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	50
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	50
Functionalized chitosan for cancer nano drug delivery	50
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	49
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	49
Advance computational tools for multiomics data learning	49
Geometric deep learning assists protein engineering. Opportunities and Challenges	49
The technology, opportunities, and challenges of Synthetic Biological Intelligence	49
A guide to the use of bioassays in exploration of natural resources	48
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	47
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics	47
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	47
Brews, fuels, and opioids: Expanding the yeast Ehrlich pathway for chemical and pharmaceutical manufacturing	46
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	46
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	46
Editorial Board	46
From traditional to AI-driven: The evolution of intelligent enzyme engineering for biocatalysis	46
Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products	46
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	46
Artificial intelligence-driven protease cleavage site prediction: Advances and challenges	46
Interpretable and explainable predictive machine learning models for data-driven protein engineering	46
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	46
Enzymatic C N bond formation and cleavage reaction: Advances and perspectives	45
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	45
Solar-powered quantum dot-biocatalyst biohybrids for semi-artificial photosynthesis: Advances in interfacial design and energy-mass transfer optimisation	44
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	44
Bioactive metabolites and extracellular vesicles from the marine chlorophyte genus Tetraselmis: Review	44
Trait drift in microalgae and applications for strain improvement	44
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	44
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	43
Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential	43
Adaptability and resiliency of Lepidoptera and their cells in culture - linking evolutionary biology to biotechnological advantage	43
The stressing point: how plants respond to environmental stimuli	43
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	43
Microbial transport systems of organic sulfur compounds: Diversity and implications for biocatalysis, healthcare, and environmental biotechnology	42
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	42
Strategies for the biological production of ectoine by using different chassis strains	42
Editorial Board	42
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	42
Design and application of microfluidics in aptamer SELEX and Aptasensors	42
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	41
Recent advances in phyto-combined remediation of heavy metal pollution in soil	41
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	41
Microbial gas fermentation technology for sustainable food protein production	41
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	41
Optogenetic technologies in translational cancer research	41
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	41
Protein representations: Encoding biological information for machine learning in biocatalysis	41
The isolation of lignin with native-like structure	40
Biopreservation of beer: Potential and constraints	40
Applications of bio-capacitance to cell culture manufacturing	40

Yeast as C1 cell factory: Transforming methanol and Formate into high-value compounds	40
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	40
Comparative evaluation of large language models for biotechnology review writing	40
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	40
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	40
Challenges and progress towards industrial recombinant protein production in yeasts: A review	40
Xylanases targeting unusual glycosidic bonds: Unlocking prebiotic xylooligosaccharides from macroalgae	40
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	40
Recent advances in microbial electron transfer-driven bio-reductive immobilization of heavy metals: Mechanisms, enhancement strategies, and perspectives	39
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	39
Plant-derived antioxidants: Biosynthesis, refining and applications	39
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	39
Engineering chaperone/usher pathway pili for surface display: Structural constraints, design principles, and biotechnological potential	39
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	39
Genetic engineering of Saccharomyces boulardii: Tools, strategies and advances for enhanced probiotic and therapeutic applications	39
Computational dual-loop frameworks bridging single-enzyme design and cascade tunnel network engineering for next-generation biosynthetic systems	39
Advances of computational protein design: Principles, strategies and applications in nutrition and health	38
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger	38
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	38
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	38
Advancements of immunoassay technology based on nanobodies	37
Circular RNAs in microalgae: Uncovering their biological significance	37
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	37
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	37
Harnessing spatial transcriptomics to understand host-parasite interactions in plants and animals	37
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	36
Membranes: The only chance for the mRNA tortoise to win, economically	36
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	36
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	36