Biotechnology Advances

Article	Citations
New perspectives into Gluconobacter-catalysed biotransformations	464
Editorial Board	241
Methanol metabolism in synthetic methylotrophic microorganisms	235
Targeted analysis of metagenomes: divide and conquer	182
Use of additives to improve collective biogas plant performances: A comprehensive review	181
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	161
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	158
Microbial cell surface engineering for high-level synthesis of bio-products	155
Biotechnological production and emerging applications of betalains: A review	149
Collagen processing with mesoscale aggregates as templates and building blocks	144
Computer-aided understanding and engineering of enzymatic selectivity	138
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	137
Recent advances in human respiratory epithelium models for drug discovery	136
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	133
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	127
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	126
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	122
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	118
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	113
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	112
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	107
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	107
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	105
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	104
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	103

Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	101
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	99
Protease engineering: Approaches, tools, and emerging trends	99
Editorial Board	95
Biotechnological applications of purine and pyrimidine deaminases	93
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	92
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	92
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	92
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	91
Advances in gene editing-led route for hybrid breeding in crops	88
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	88
Biological valorization of lignin to flavonoids	86
Polyethylene biodegradation: A multifaceted approach	86
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	85
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	85
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	83
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	83
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	80
Growth-coupled high throughput selection for directed enzyme evolution	78
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	78
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	77
Recent advances in machine learning applications in metabolic engineering	77
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	77
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	76
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	75
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	74
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	74
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	73
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	73
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	73
Stress response in Rhodococcus strains	72
Recent advances in research for potential utilization of unexplored lichen metabolites	71
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	70
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	69
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	69
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	68
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	68
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	67
Resource allocation in mammalian systems	66
Research progress of engineering microbial cell factories for pigment production	66
Rutinosidase and other diglycosidases: Rising stars in biotechnology	64
Editorial Board	64
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	63
Recent advances in engineering synthetic biomolecular condensates	63
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	63
Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	63
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	62
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	62
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	62
Detection, production, modification, and application of arylsulfatases	62

Genetic engineering of bacteriophages: Key concepts, strategies, and applications	61
Recent advances in lipid metabolic engineering of oleaginous yeasts	61
Engineering membrane architecture for biotechnological applications	61
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	61
Recent advances in enzyme-enhanced immunosensors	61
Harnessing the biomolecular mechanisms of marine biomineralisation for carbon sequestration	61
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	60
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	60
Editorial Board	59
Advance computational tools for multiomics data learning	58
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	58
Biotechnological applications of type 1 secretion systems	57
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	57
Bacterial cellulose and its potential for biomedical applications	57
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	57
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	57
Optimizing microbial networks through metabolic bypasses	56
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	56
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	56
Principles of antibodies with ultralong complementarity-determining regions and picobodies	56
Enzyme miniaturization: Revolutionizing future biocatalysts	55
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	55
Functionalized chitosan for cancer nano drug delivery	54
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	54
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	54
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	53
Interpretable and explainable predictive machine learning models for data-driven protein engineering	53
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	53
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	52
A guide to the use of bioassays in exploration of natural resources	52
The technology, opportunities, and challenges of Synthetic Biological Intelligence	52
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	52
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	52
Trait drift in microalgae and applications for strain improvement	51
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	51
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	51
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	51
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	50
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	50
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	50
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	49
Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential	49
Bioactive metabolites and extracellular vesicles from the marine chlorophyte genus Tetraselmis: Review	49
Enzymatic C N bond formation and cleavage reaction: Advances and perspectives	49
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	49
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	49
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	48
Biofilm control by interfering with c-di-GMP metabolism and signaling	47
Protein representations: Encoding biological information for machine learning in biocatalysis	47
Biopreservation of beer: Potential and constraints	46
Microbial gas fermentation technology for sustainable food protein production	46
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	46
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	46
Microbial transport systems of organic sulfur compounds: Diversity and implications for biocatalysis, healthcare, and environmental biotechnology	46
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	45
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	45
Strategies for the biological production of ectoine by using different chassis strains	45
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	44
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	44
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	43
Optogenetic technologies in translational cancer research	43
Design and application of microfluidics in aptamer SELEX and Aptasensors	43
Challenges and progress towards industrial recombinant protein production in yeasts: A review	42
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	41
The isolation of lignin with native-like structure	41
Plants arabinogalactans: From structures to physico-chemical and biological properties	41
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	40
Recent advances in phyto-combined remediation of heavy metal pollution in soil	40
Applications of bio-capacitance to cell culture manufacturing	40
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	40
Yeast as C1 cell factory: Transforming methanol and Formate into high-value compounds	39
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	39
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	39
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	39
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	39
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	39
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	38
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	38
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	38
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	38

Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	38
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	38
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	38
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	38
Refactoring transcription factors for metabolic engineering	37
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	37
In vitro models of gut digestion across childhood: current developments, challenges and future trends	37
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	37
From natural to synthetic: Promoter engineering in yeast expression systems	37
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	37
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	37
Advances in production and application technologies of minicells: A review	36
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	36
Advances of computational protein design: Principles, strategies and applications in nutrition and health	36
Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil healt	35
Computational advances in biosynthetic gene cluster discovery and prediction	35
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	35
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	35