Biotechnology Advances

Article	Citations
Editorial Board	274
Editorial Board	200
Methanol metabolism in synthetic methylotrophic microorganisms	176
Targeted analysis of metagenomes: divide and conquer	157
New perspectives into Gluconobacter-catalysed biotransformations	156
Microbial cell surface engineering for high-level synthesis of bio-products	151
Collagen processing with mesoscale aggregates as templates and building blocks	151
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	133
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	133
Biotechnological production and emerging applications of betalains: A review	132
Decoding microbial interactions: Interaction networks and regulatory strategies for medium-chain fatty acid biosynthesis through anaerobic chain elongation	131
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	131
Recent advances in human respiratory epithelium models for drug discovery	117
Computer-aided understanding and engineering of enzymatic selectivity	115
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	114
Use of additives to improve collective biogas plant performances: A comprehensive review	113
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	108
Benzylisoquinoline alkaloid production: Moving from crop farming to chemical and biosynthesis	107
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	103
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	102
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	101
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	99
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	97
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	96
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	95

Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	93
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	93
Editorial Board	92
The path to biotechnological singularity: Current breakthroughs and outlook	92
Editorial Board	92
Emerging research insights and future perspectives on the advancement of eccDNA: A comprehensive review	90
Biotechnological applications of purine and pyrimidine deaminases	88
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	86
Polyethylene biodegradation: A multifaceted approach	86
Plant cell wall biosynthesis: Immune signaling, genome editing, and physiological implications for biomass valorization	84
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	83
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	83
Growth-coupled high throughput selection for directed enzyme evolution	81
Advances in gene editing-led route for hybrid breeding in crops	81
Haloferax and the Haloferacaceae: Potential role in bioindustry	81
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	81
Protease engineering: Approaches, tools, and emerging trends	80
Biological valorization of lignin to flavonoids	79
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	76
Recent advances in machine learning applications in metabolic engineering	76
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	76
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	75
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	75
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	73
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	72
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	71
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	71
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	70
Recent advances in research for potential utilization of unexplored lichen metabolites	70
Rutinosidase and other diglycosidases: Rising stars in biotechnology	70
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	70
Resource allocation in mammalian systems	70
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	67
Loop engineering in enzymes from structure to function: Mechanisms, methodologies, and engineering strategies	66
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	65
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	65
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	65
Research progress of engineering microbial cell factories for pigment production	65
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	64
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	64
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	63
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	63
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	63
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	63
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	62
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	62
Recent advances in engineering synthetic biomolecular condensates	61
Editorial Board	61
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	61
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	61

Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	60
Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications	60
Microalgal bioengineering for futuristic applications in synthetic and space biology	59
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	58
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	58
Engineering membrane architecture for biotechnological applications	58
Machine learning in predictive biocatalysis: A comparative review of methods and applications	58
Harnessing the biomolecular mechanisms of marine biomineralisation for carbon sequestration	57
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	57
Detection, production, modification, and application of arylsulfatases	57
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	57
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	56
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	56
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	55
Enzyme miniaturization: Revolutionizing future biocatalysts	54
Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects	54
Editorial Board	54
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	54
Optimizing microbial networks through metabolic bypasses	53
Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products	53
Smart culture medium optimization for recombinant protein production: Experimental, modeling, and AI/ML-driven strategies	53
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	53
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	52
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics	52
Interpretable and explainable predictive machine learning models for data-driven protein engineering	52
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	52
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	51
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	51
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	50
The technology, opportunities, and challenges of Synthetic Biological Intelligence	50
Principles of antibodies with ultralong complementarity-determining regions and picobodies	49
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	49
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	49
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	49
Advance computational tools for multiomics data learning	49
Brews, fuels, and opioids: Expanding the yeast Ehrlich pathway for chemical and pharmaceutical manufacturing	48
Functionalized chitosan for cancer nano drug delivery	48
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	46
Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential	46
A guide to the use of bioassays in exploration of natural resources	46
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	46
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	46
Enzymatic C N bond formation and cleavage reaction: Advances and perspectives	45
Trait drift in microalgae and applications for strain improvement	45
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	44
Bioactive metabolites and extracellular vesicles from the marine chlorophyte genus Tetraselmis: Review	44
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	44
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	44
Biofilm control by interfering with c-di-GMP metabolism and signaling	43
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	43
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	42
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	42
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	41
The stressing point: how plants respond to environmental stimuli	41
Microbial transport systems of organic sulfur compounds: Diversity and implications for biocatalysis, healthcare, and environmental biotechnology	41
Adaptability and resiliency of Lepidoptera and their cells in culture - linking evolutionary biology to biotechnological advantage	41
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	40
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	40
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	40
Protein representations: Encoding biological information for machine learning in biocatalysis	39
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	39
Optogenetic technologies in translational cancer research	39
Biopreservation of beer: Potential and constraints	39
Challenges and progress towards industrial recombinant protein production in yeasts: A review	38
Strategies for the biological production of ectoine by using different chassis strains	38
The isolation of lignin with native-like structure	38
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	38
Design and application of microfluidics in aptamer SELEX and Aptasensors	38
Recent advances in phyto-combined remediation of heavy metal pollution in soil	37
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	37
Genetic engineering of Saccharomyces boulardii: Tools, strategies and advances for enhanced probiotic and therapeutic applications	37
Microbial gas fermentation technology for sustainable food protein production	37
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	37
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	37
Yeast as C1 cell factory: Transforming methanol and Formate into high-value compounds	37
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	37
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	36
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	36
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	36
Applications of bio-capacitance to cell culture manufacturing	36

Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	36
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	35
Advances of computational protein design: Principles, strategies and applications in nutrition and health	35
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	35
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	35
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	35
Advances in production and application technologies of minicells: A review	35
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	35
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	35
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	35
In vitro models of gut digestion across childhood: current developments, challenges and future trends	35
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	34
Circular RNAs in microalgae: Uncovering their biological significance	34
Submission to Biotechnology Advances Main Manuscript entitled: Perspective: “Membranes: The only chance for the mRNA tortoise to win, economically”	34
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	34
From natural to synthetic: Promoter engineering in yeast expression systems	34
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	33
Refactoring transcription factors for metabolic engineering	33
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger	33
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	33
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	32
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	32
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	32
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	32
Understanding low-phosphate stress responses in plants: Opportunities for genome editing to improve phosphorous use efficiency (PUE)	32
Editorial Board	32
Computational advances in biosynthetic gene cluster discovery and prediction	32
Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil healt	32
Cellular variability as a driver for bioprocess innovation and optimization	32
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	31
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	31
Revolutionizing agroindustry: Towards the industrial application of antimicrobial peptides against pathogens and pests	31
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	31
Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing	31
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	31
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	31
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	31
Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics	30
Decoding bioprocesses with transcriptomics: current status and future potential	30
From formate oxidation to CO₂ reduction: The role of formate dehydrogenase in sustainable carbon utilization	30
Epigenetic therapy for leukemias: a comprehensive review from biological markers to practice	30
Methods of in vitro study of galectin-glycomaterial interaction	30
Yeast surface display technology: Mechanisms, applications, and perspectives	30
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	30
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	30
Advances in biosensors for microbial biosynthesis of amino acids and their derivatives	30
Bacterial membrane vesicle functions, laboratory methods, and applications	30
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	29
Design and biocatalytic applications of genetically fused multifunctional enzymes	29
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	29
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	29
Molecular imprinting technology for biomedical applications	29
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	29
Optimization strategies for CO2 biological fixation	29
Revolutionizing keratinase science: Biocatalytic advances, sustainable innovation, and industrial perspectives	29
Biopolymers and their derivatives: Key components of advanced biomedical technologies	29
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	29
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	29
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	29
Strategies for improving antimicrobial peptide production	28
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	28
Editorial Board	28
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	28
Identification, characterization, and engineering of glycosylation in thrombolytics	28
Nucleic acid-responsive smart systems for controlled cargo delivery	28
Editorial Board	28
Editorial Board	27
Baculovirus dual-phase infection strategy and biotechnological applications: A structural and regulatory review	27
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	27
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	27
Recent advances in fed-batch microscale bioreactor design	27
A review on the expanding biotechnological frontier of Pedobacter	27
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	27
Morphology engineering facilitates constructing efficient cell factories	27
Current advancement in AI-integrated drug discovery: Methods and applications	27
Chimeric enzymes in the pulp and paper making industry: Current developments	27
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	26
Insights on the emerging biotechnology of histidine-rich peptides	26
Biophotovoltaics: Recent advances and perspectives	26
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	26
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	26
Engineering status of protein for improving microbial cell factories	26
Engineering catalytically promiscuous enzymes to serve new functions	26
Next generation probiotics: Engineering live biotherapeutics	26
The role of orphan crops in the transition to nutritional quality-oriented crop improvement	25
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	25
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	25
Engineering conditional protein-protein interactions for dynamic cellular control	25
Production and characterization of starch-lignin based materials: A review	25
Expanding the horizon of transient CAR T therapeutics using virus-free technology	25
Role of distal sites in enzyme engineering	25
Challenges of polyethylene (PE) biodegradation – A perspective	24
4D printing: Perspectives for the production of sustainable plastics for agriculture	24
Susceptibility genes in plants: from molecular mechanisms to ecological implications for disease resistance	24
Extremophilic lipases for industrial applications: A general review	24