Biotechnology Advances

Article	Citations
Editorial Board	261
Editorial Board	255
Methanol metabolism in synthetic methylotrophic microorganisms	192
Collagen processing with mesoscale aggregates as templates and building blocks	173
Targeted analysis of metagenomes: divide and conquer	166
Use of additives to improve collective biogas plant performances: A comprehensive review	154
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	151
Computer-aided understanding and engineering of enzymatic selectivity	148
New perspectives into Gluconobacter-catalysed biotransformations	147
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	137
Biotechnological production and emerging applications of betalains: A review	129
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	129
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	127
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	124
Recent advances in human respiratory epithelium models for drug discovery	122
Microbial cell surface engineering for high-level synthesis of bio-products	110
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	110
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	109
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	108
Benzylisoquinoline alkaloid production: Moving from crop farming to chemical and biosynthesis	106
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	105
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	102
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	101
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	98
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	96

Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	96
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	96
The path to biotechnological singularity: Current breakthroughs and outlook	94
Editorial Board	94
Polyethylene biodegradation: A multifaceted approach	93
Biotechnological applications of purine and pyrimidine deaminases	90
Editorial Board	90
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	90
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	89
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	89
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	86
Recent advances in machine learning applications in metabolic engineering	86
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	86
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	85
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	83
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	83
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	82
Biological valorization of lignin to flavonoids	80
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	80
Advances in gene editing-led route for hybrid breeding in crops	79
Haloferax and the Haloferacaceae: Potential role in bioindustry	79
Growth-coupled high throughput selection for directed enzyme evolution	78
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	77
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	75
Plant cell wall biosynthesis: Immune signaling, genome editing, and physiological implications for biomass valorization	75
Emerging research insights and future perspectives on the advancement of eccDNA: A comprehensive review	75
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	74
Protease engineering: Approaches, tools, and emerging trends	74
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	72
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	72
Resource allocation in mammalian systems	72
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	72
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	72
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	71
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	71
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	69
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	68
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	68
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	68
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	68
Research progress of engineering microbial cell factories for pigment production	67
Loop engineering in enzymes from structure to function: Mechanisms, methodologies, and engineering strategies	65
Recent advances in research for potential utilization of unexplored lichen metabolites	65
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	64
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	64
Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications	63
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	63
Rutinosidase and other diglycosidases: Rising stars in biotechnology	63
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	63
Stress response in Rhodococcus strains	63

Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	62
Microalgal bioengineering for futuristic applications in synthetic and space biology	62
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	61
Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	61
Editorial Board	61
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	61
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	61
Recent advances in enzyme-enhanced immunosensors	61
Recent advances in engineering synthetic biomolecular condensates	60
Detection, production, modification, and application of arylsulfatases	59
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	59
Engineering membrane architecture for biotechnological applications	59
Harnessing the biomolecular mechanisms of marine biomineralisation for carbon sequestration	58
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	58
Machine learning in predictive biocatalysis: A comparative review of methods and applications	58
Recent advances in lipid metabolic engineering of oleaginous yeasts	58
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	57
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	57
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	56
Biotechnological applications of type 1 secretion systems	55
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	55
Editorial Board	55
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	55
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	54
Brews, fuels, and opioids: Expanding the yeast Ehrlich pathway for chemical and pharmaceutical manufacturing	54
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics	54
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	53
Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products	52
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	52
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	52
Optimizing microbial networks through metabolic bypasses	52
Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects	52
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	51
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	51
Principles of antibodies with ultralong complementarity-determining regions and picobodies	51
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	50
Interpretable and explainable predictive machine learning models for data-driven protein engineering	50
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	50
The technology, opportunities, and challenges of Synthetic Biological Intelligence	50
Functionalized chitosan for cancer nano drug delivery	50
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	49
Bacterial cellulose and its potential for biomedical applications	49
A guide to the use of bioassays in exploration of natural resources	49
Enzyme miniaturization: Revolutionizing future biocatalysts	49
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	48
Bioactive metabolites and extracellular vesicles from the marine chlorophyte genus Tetraselmis: Review	48
Advance computational tools for multiomics data learning	48
Enzymatic C N bond formation and cleavage reaction: Advances and perspectives	48
Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential	48
The stressing point: how plants respond to environmental stimuli	47
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	47
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	46
Trait drift in microalgae and applications for strain improvement	45
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	45
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	45
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	44
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	44
Biofilm control by interfering with c-di-GMP metabolism and signaling	44
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	44
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	43
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	43
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	42
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	41
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	41
Optogenetic technologies in translational cancer research	41
Microbial gas fermentation technology for sustainable food protein production	41
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	41
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	41
Biopreservation of beer: Potential and constraints	41
Plants arabinogalactans: From structures to physico-chemical and biological properties	41
Strategies for the biological production of ectoine by using different chassis strains	40
Protein representations: Encoding biological information for machine learning in biocatalysis	40
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	40
Yeast as C1 cell factory: Transforming methanol and Formate into high-value compounds	39
Design and application of microfluidics in aptamer SELEX and Aptasensors	39
The isolation of lignin with native-like structure	39
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	39
Recent advances in phyto-combined remediation of heavy metal pollution in soil	39
Challenges and progress towards industrial recombinant protein production in yeasts: A review	39
Applications of bio-capacitance to cell culture manufacturing	38

Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	38
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	38
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	37
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	37
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	37
Genetic engineering of Saccharomyces boulardii: Tools, strategies and advances for enhanced probiotic and therapeutic applications	37
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	37
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	37
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	36
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	36
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	36
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger	36
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	36
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	35
Advances in production and application technologies of minicells: A review	35
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	35
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	35
In vitro models of gut digestion across childhood: current developments, challenges and future trends	35
Advances of computational protein design: Principles, strategies and applications in nutrition and health	35
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	34
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	34
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	34
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	34
Refactoring transcription factors for metabolic engineering	33
Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil healt	33
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	33
Computational advances in biosynthetic gene cluster discovery and prediction	33
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	33
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	33
From natural to synthetic: Promoter engineering in yeast expression systems	33
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	33
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	32
Editorial Board	32
Revolutionizing agroindustry: Towards the industrial application of antimicrobial peptides against pathogens and pests	32
Understanding low-phosphate stress responses in plants: Opportunities for genome editing to improve phosphorous use efficiency (PUE)	32
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	32
Editorial Board	32
Cellular variability as a driver for bioprocess innovation and optimization	32
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	32
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	32
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	31
Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing	31
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	31
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	31
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	31
Methods of in vitro study of galectin-glycomaterial interaction	30
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	30
Epigenetic therapy for leukemias: a comprehensive review from biological markers to practice	30
Biopolymers and their derivatives: Key components of advanced biomedical technologies	30
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	30
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	30
Revolutionizing keratinase science: Biocatalytic advances, sustainable innovation, and industrial perspectives	30
Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics	30
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	30
From formate oxidation to CO₂ reduction: The role of formate dehydrogenase in sustainable carbon utilization	29
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	29
Molecular imprinting technology for biomedical applications	29
Design and biocatalytic applications of genetically fused multifunctional enzymes	29
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	29
Strategies for improving antimicrobial peptide production	29
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	29
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	29
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	29
Optimization strategies for CO2 biological fixation	29
Yeast surface display technology: Mechanisms, applications, and perspectives	28
Bacterial membrane vesicle functions, laboratory methods, and applications	28
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	28
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	28
Nucleic acid-responsive smart systems for controlled cargo delivery	28
Baculovirus dual-phase infection strategy and biotechnological applications: A structural and regulatory review	28
Morphology engineering facilitates constructing efficient cell factories	28
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	28
Editorial Board	28
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	28
Editorial Board	27
Identification, characterization, and engineering of glycosylation in thrombolytics	27
Engineering catalytically promiscuous enzymes to serve new functions	27
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	27
Chimeric enzymes in the pulp and paper making industry: Current developments	27
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	27
Editorial Board	27
Engineering status of protein for improving microbial cell factories	27
A review on the expanding biotechnological frontier of Pedobacter	27
Engineering lignin pathway and plant cell wall modification and genome editing for advanced renewable bioenergy and material applications	26
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	26
Next generation probiotics: Engineering live biotherapeutics	26
Challenges of polyethylene (PE) biodegradation – A perspective	26
Production and characterization of starch-lignin based materials: A review	26
Recent advances in fed-batch microscale bioreactor design	26
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	26
Role of distal sites in enzyme engineering	26
Current advancement in AI-integrated drug discovery: Methods and applications	26
Biophotovoltaics: Recent advances and perspectives	26
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	26
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	26