Biotechnology Advances

Article	Citations
Editorial Board	321
Editorial Board	183
Use of additives to improve collective biogas plant performances: A comprehensive review	171
Targeted analysis of metagenomes: divide and conquer	166
Collagen processing with mesoscale aggregates as templates and building blocks	161
Biotechnological production and emerging applications of betalains: A review	157
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	154
Decoding microbial interactions: Interaction networks and regulatory strategies for medium-chain fatty acid biosynthesis through anaerobic chain elongation	140
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	134
Biotechnological advances in key regulatory genes of phenylpropanoid and terpenoid biosynthesis pathways in Panax ginseng: Current insights and future prospects	130
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	128
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	123
Machine learning methods for small data and upstream bioprocessing applications: A comprehensive review	118
Methanol metabolism in synthetic methylotrophic microorganisms	118
New perspectives into Gluconobacter-catalysed biotransformations	118
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	118
Benzylisoquinoline alkaloid production: Moving from crop farming to chemical and biosynthesis	114
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	113
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	113
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	111
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	107
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	103
Microbial computing: Review and Perspectives	103
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	102
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	100

Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	100
Editorial Board	96
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	96
Biological valorization of lignin to flavonoids	92
The path to biotechnological singularity: Current breakthroughs and outlook	90
Plant cell wall biosynthesis: Immune signaling, genome editing, and physiological implications for biomass valorization	90
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	89
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	86
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	85
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	84
Advances in gene editing-led route for hybrid breeding in crops	82
Droplet microfluidics for single-cell analysis: From improved cell encapsulation and sorting technologies to innovative applications	82
Emerging research insights and future perspectives on the advancement of eccDNA: A comprehensive review	81
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	81
Biotechnological applications of purine and pyrimidine deaminases	78
Advances and challenges in enzymatic rubber degradation: Exploring genetic, molecular, and biotechnological aspects	77
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	77
Advances in transcription factor-based biosensors for natural product biosynthesis: Optimization, emerging technologies, and future prospects	76
Recent advances in machine learning applications in metabolic engineering	76
Haloferax and the Haloferacaceae: Potential role in bioindustry	76
Computational methods for spatial multi-omics integration	75
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	75
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	75
Growth-coupled high throughput selection for directed enzyme evolution	74
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	74
Protease engineering: Approaches, tools, and emerging trends	74
Polyethylene biodegradation: A multifaceted approach	74
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	73
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	72
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	71
Editorial Board	71
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	71
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	70
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	70
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	69
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	69
The fungal cure: Harnessing mycelial approach as sustainable green solution for industrial waste treatment	68
Optimizing prime editing: Advances in efficiency enhancement	67
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	67
Recent advances in research for potential utilization of unexplored lichen metabolites	67
Resource allocation in mammalian systems	66
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	65
Research progress of engineering microbial cell factories for pigment production	65
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	63
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	63
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	62
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	62
Loop engineering in enzymes from structure to function: Mechanisms, methodologies, and engineering strategies	61
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	61
Rutinosidase and other diglycosidases: Rising stars in biotechnology	61

Editorial Board	59
Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications	59
UGT708 glycosyltransferases: Nature's architects of C-glycosides	59
Harnessing the biomolecular mechanisms of marine biomineralisation for carbon sequestration	58
Microalgal bioengineering for futuristic applications in synthetic and space biology	58
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	57
Detection, production, modification, and application of arylsulfatases	56
Construction and applications of methanol bio-converting cell factories	56
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	56
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	55
Machine learning in predictive biocatalysis: A comparative review of methods and applications	53
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	53
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	53
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	53
Recent advances in engineering synthetic biomolecular condensates	53
Engineering membrane architecture for biotechnological applications	52
Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	52
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	51
Flavin reductases in two-component systems: Mechanistic insights, structural classification, and biotechnological advances	51
Editorial Board	51
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	51
Microbial collaboration in bioelectrochemical systems: Mechanisms and advances towards sustainable biobased products	51
Corrigendum to ‘Expanding biotechnological applications of Yarrowia lipolytica: Key advances in the past decade’ [Biotechnol. Adv. 88 (2026) 108835]	51
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	50
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	50
Principles of antibodies with ultralong complementarity-determining regions and picobodies	50
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	49
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	49
A guide to the use of bioassays in exploration of natural resources	49
Brews, fuels, and opioids: Expanding the yeast Ehrlich pathway for chemical and pharmaceutical manufacturing	48
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	47
Optimizing microbial networks through metabolic bypasses	47
Geometric deep learning assists protein engineering. Opportunities and Challenges	46
Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products	46
Functionalized chitosan for cancer nano drug delivery	46
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	46
Enzyme miniaturization: Revolutionizing future biocatalysts	46
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	46
Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects	45
From traditional to AI-driven: The evolution of intelligent enzyme engineering for biocatalysis	45
Smart culture medium optimization for recombinant protein production: Experimental, modeling, and AI/ML-driven strategies	45
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	44
The technology, opportunities, and challenges of Synthetic Biological Intelligence	44
Enzymatic C N bond formation and cleavage reaction: Advances and perspectives	43
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	43
Interpretable and explainable predictive machine learning models for data-driven protein engineering	43
Solar-powered quantum dot-biocatalyst biohybrids for semi-artificial photosynthesis: Advances in interfacial design and energy-mass transfer optimisation	43
Advance computational tools for multiomics data learning	43
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	43
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	43
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics	43
Bioactive metabolites and extracellular vesicles from the marine chlorophyte genus Tetraselmis: Review	42
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	42
Trait drift in microalgae and applications for strain improvement	42
Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential	42
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	42
The stressing point: how plants respond to environmental stimuli	42
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	42
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	42
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	41
Editorial Board	41
Challenges and progress towards industrial recombinant protein production in yeasts: A review	41
Biopreservation of beer: Potential and constraints	41
Adaptability and resiliency of Lepidoptera and their cells in culture - linking evolutionary biology to biotechnological advantage	41
Strategies for the biological production of ectoine by using different chassis strains	40
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	40
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	40
Microbial gas fermentation technology for sustainable food protein production	40
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	40
Optogenetic technologies in translational cancer research	40
Microbial transport systems of organic sulfur compounds: Diversity and implications for biocatalysis, healthcare, and environmental biotechnology	39
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	39
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	39
The isolation of lignin with native-like structure	39
Protein representations: Encoding biological information for machine learning in biocatalysis	39
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	39
Recent advances in phyto-combined remediation of heavy metal pollution in soil	39
Design and application of microfluidics in aptamer SELEX and Aptasensors	39
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	39
Genetic engineering of Saccharomyces boulardii: Tools, strategies and advances for enhanced probiotic and therapeutic applications	39

Recent advances in microbial electron transfer-driven bio-reductive immobilization of heavy metals: Mechanisms, enhancement strategies, and perspectives	38
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	38
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	38
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	38
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	38
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	37
Applications of bio-capacitance to cell culture manufacturing	37
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	37
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	37
Yeast as C1 cell factory: Transforming methanol and Formate into high-value compounds	37
Xylanases targeting unusual glycosidic bonds: Unlocking prebiotic xylooligosaccharides from macroalgae	37
Comparative evaluation of large language models for biotechnology review writing	37
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	37
Recent advances in the covalent-bond-based peptide tagging systems and their applications	36
Advances in production and application technologies of minicells: A review	36
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	36
Circular RNAs in microalgae: Uncovering their biological significance	36
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger	36
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	36
Advances of computational protein design: Principles, strategies and applications in nutrition and health	36
Membranes: The only chance for the mRNA tortoise to win, economically	35
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	35
Harnessing spatial transcriptomics to understand host-parasite interactions in plants and animals	35
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	34
Refactoring transcription factors for metabolic engineering	34
From natural to synthetic: Promoter engineering in yeast expression systems	34
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	34
Computational advances in biosynthetic gene cluster discovery and prediction	33
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	33
Advancements of immunoassay technology based on nanobodies	33
Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil healt	33
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	33
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	33
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	33
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	33
Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing	32
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	32
Understanding low-phosphate stress responses in plants: Opportunities for genome editing to improve phosphorous use efficiency (PUE)	32
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	32
Editorial Board	32
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	32
Revolutionizing agroindustry: Towards the industrial application of antimicrobial peptides against pathogens and pests	32
Vanadium-dependent haloperoxidases: Recent advances and perspectives	32
Cellular variability as a driver for bioprocess innovation and optimization	32
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	32
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	32
Epigenetic therapy for leukemias: a comprehensive review from biological markers to practice	31
Glycobiohydrolases at the forefront of lignocellulose saccharification: A review on substrate specificities and structure-function properties	31
Revolutionizing keratinase science: Biocatalytic advances, sustainable innovation, and industrial perspectives	31
Molecular imprinting technology for biomedical applications	31
Optimization strategies for CO2 biological fixation	31
Decoding bioprocesses with transcriptomics: current status and future potential	31
Advances in biosensors for microbial biosynthesis of amino acids and their derivatives	31
Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics	31
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	30
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	30
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	30
Design and biocatalytic applications of genetically fused multifunctional enzymes	30
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	30
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	30
Viral clearance in biopharmaceutical manufacturing: Current strategies, challenges, and future directions	30
Methods of in vitro study of galectin-glycomaterial interaction	30
Biopolymers and their derivatives: Key components of advanced biomedical technologies	30
From formate oxidation to CO₂ reduction: The role of formate dehydrogenase in sustainable carbon utilization	29
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	29
Strategies for improving antimicrobial peptide production	29
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	29
Flavin-dependent enzymes as multifunctional biocatalysts: A mechanistic perspective	28
Editorial Board	28
Yeast surface display technology: Mechanisms, applications, and perspectives	28
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	28
Editorial Board	28
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	28
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	28
Biophotovoltaics: Recent advances and perspectives	27
Morphology engineering facilitates constructing efficient cell factories	27
Editorial Board	27
Engineering lignin pathway, plant cell wall modification, and genome editing for advanced renewable bioenergy and material applications	27
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	27
Nucleic acid-responsive smart systems for controlled cargo delivery	27
Engineering status of protein for improving microbial cell factories	27
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	27
Baculovirus dual-phase infection strategy and biotechnological applications: A structural and regulatory review	27
Current advancement in AI-integrated drug discovery: Methods and applications	26
Chimeric enzymes in the pulp and paper making industry: Current developments	26
Identification, characterization, and engineering of glycosylation in thrombolytics	26
Reporter systems in actinomycetes: Versatile tools for natural product discovery and production	26
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	26
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	26
Next generation probiotics: Engineering live biotherapeutics	26
Production and characterization of starch-lignin based materials: A review	26
A review on the expanding biotechnological frontier of Pedobacter	26
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	26
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	26
Engineering catalytically promiscuous enzymes to serve new functions	25