Biotechnology Advances

Article	Citations
Editorial Board	498
Use of additives to improve collective biogas plant performances: A comprehensive review	427
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	212
Collagen processing with mesoscale aggregates as templates and building blocks	200
Recent advances in human respiratory epithelium models for drug discovery	178
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	176
Microbial cell surface engineering for high-level synthesis of bio-products	175
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	174
New perspectives into Gluconobacter-catalysed biotransformations	154
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	151
Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects	143
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	139
Computer-aided understanding and engineering of enzymatic selectivity	127
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	125
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	119
Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	117
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	115
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	114
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	112
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	112
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	111
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	109
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	102
Charting the landscape of RNA polymerases to unleash their potential in strain improvement	96
Proteomic and Glyco(proteo)mic tools in the profiling of cardiac progenitors and pluripotent stem cell derived cardiomyocytes: Accelerating translation into therapy	95

Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	92
Microbial starch debranching enzymes: Developments and applications	90
Biological valorization of lignin to flavonoids	89
Growth-coupled high throughput selection for directed enzyme evolution	85
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	85
Recent advances in machine learning applications in metabolic engineering	84
Editorial Board	82
Enhancing single-cell bioconversion efficiency by harnessing nanosecond pulsed electric field processing	81
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	80
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	80
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	80
Biotechnological applications of purine and pyrimidine deaminases	78
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	78
Using machine learning approaches for multi-omics data analysis: A review	77
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	76
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	76
Pseudomonas aeruginosa biofilms and their partners in crime	76
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	75
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	75
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	74
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	72
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	72
Recent advances in research for potential utilization of unexplored lichen metabolites	70
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	70
Rutinosidase and other diglycosidases: Rising stars in biotechnology	70
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	68
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	68
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	66
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	66
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	65
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	64
Resource allocation in mammalian systems	64
Stress response in Rhodococcus strains	64
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	64
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	63
Research progress of engineering microbial cell factories for pigment production	63
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	63
Editorial Board	62
Objectives, benefits and challenges of bioreactor systems for the clinical-scale expansion of T lymphocyte cells	60
Recent advances in lipid metabolic engineering of oleaginous yeasts	59
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	59
Detection, production, modification, and application of arylsulfatases	59
Recent advances in enzyme-enhanced immunosensors	59
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	59
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	59
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	58
Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models	58
Recent advances in engineering synthetic biomolecular condensates	58
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	58
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	58

Engineering membrane architecture for biotechnological applications	58
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	57
Interpretable and explainable predictive machine learning models for data-driven protein engineering	57
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	57
Editorial Board	55
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	55
Editorial Board	55
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	55
Biotechnological applications of type 1 secretion systems	54
A guide to the use of bioassays in exploration of natural resources	53
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	53
Optimizing microbial networks through metabolic bypasses	52
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	52
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	51
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	51
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	51
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	51
Advance computational tools for multiomics data learning	50
Conversion of inulin-rich raw plant biomass to 2,5-furandicarboxylic acid (FDCA): Progress and challenge towards biorenewable plastics	50
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	49
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	49
Bacterial cellulose and its potential for biomedical applications	48
The technology, opportunities, and challenges of Synthetic Biological Intelligence	48
Functionalized chitosan for cancer nano drug delivery	48
Principles of antibodies with ultralong complementarity-determining regions and picobodies	48
Improving the performance of machine learning models for biotechnology: The quest for deus ex machina	47
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	47
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	47
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	47
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	47
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	46
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	46
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	46
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	46
New trends in the biocatalytic production of nucleosidic active pharmaceutical ingredients using 2′-deoxyribosyltransferases	46
Biofilm control by interfering with c-di-GMP metabolism and signaling	46
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	46
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	46
Strategies for the biological production of ectoine by using different chassis strains	46
Trait drift in microalgae and applications for strain improvement	46
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	46
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	45
Challenges and progress towards industrial recombinant protein production in yeasts: A review	45
Optogenetic technologies in translational cancer research	45
Biopreservation of beer: Potential and constraints	45
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	44
Protein representations: Encoding biological information for machine learning in biocatalysis	44
Design and application of microfluidics in aptamer SELEX and Aptasensors	44
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	44
Microbial gas fermentation technology for sustainable food protein production	44
Plants arabinogalactans: From structures to physico-chemical and biological properties	44
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	43
The isolation of lignin with native-like structure	42
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	42
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	42
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	42
Recent advances in phyto-combined remediation of heavy metal pollution in soil	42
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	40
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	40
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	40
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	40
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	40
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	39
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	38
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	38
Applications of bio-capacitance to cell culture manufacturing	38
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	38
Fine-tuning gene expression for improved biosynthesis of natural products: From transcriptional to post-translational regulation	38
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	37
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	36
The emerging role of cellular post-translational modifications in modulating growth and productivity of recombinant Chinese hamster ovary cells	36
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	36
Computational advances in biosynthetic gene cluster discovery and prediction	36
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	36
From natural to synthetic: Promoter engineering in yeast expression systems	36
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	36
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	35
In vitro models of gut digestion across childhood: current developments, challenges and future trends	35
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	35
Refactoring transcription factors for metabolic engineering	35

Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	34
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	34
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	34
Recent advances in microbial production of high-value compounds in the tetrapyrrole biosynthesis pathway	34
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	34
Editorial Board	33
Editorial Board	33
Cellular variability as a driver for bioprocess innovation and optimization	33
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	32
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	32
Engineering microorganisms for the biosynthesis of dicarboxylic acids	32
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	32
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	32
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	31
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	31
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	31
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	31
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	31
Methods of in vitro study of galectin-glycomaterial interaction	31
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	31
Yeast surface display technology: Mechanisms, applications, and perspectives	30
Strategies for improving antimicrobial peptide production	30
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	30
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	30
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	30
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	30
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	30
Design and biocatalytic applications of genetically fused multifunctional enzymes	29
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	29
N-oxygenation of amino compounds: Early stages in its application to the biocatalyzed preparation of bioactive compounds	29
Optimization strategies for CO2 biological fixation	29
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	29
Bacterial membrane vesicle functions, laboratory methods, and applications	29
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	29
Biopolymers and their derivatives: Key components of advanced biomedical technologies	29
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	28
Editorial Board	28
Editorial Board	28
Production and characterization of starch-lignin based materials: A review	28
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	28
Recent advances in synthetic biology toolkits and metabolic engineering of Ralstonia eutropha H16 for production of value-added chemicals	28
Molecular imprinting technology for biomedical applications	28
Nucleic acid-responsive smart systems for controlled cargo delivery	28
Identification, characterization, and engineering of glycosylation in thrombolytics	28
Chimeric enzymes in the pulp and paper making industry: Current developments	28
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	28
Editorial Board	28
Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration	28
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	28
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	27
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	27
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	27
Next generation probiotics: Engineering live biotherapeutics	27
Engineering status of protein for improving microbial cell factories	27
Recent advances in fed-batch microscale bioreactor design	27
Biophotovoltaics: Recent advances and perspectives	27
Insights on the emerging biotechnology of histidine-rich peptides	27
Extremophilic lipases for industrial applications: A general review	26
Editorial Board	26
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	26
Biosynthesis of alkanes/alkenes from fatty acids or derivatives (triacylglycerols or fatty aldehydes)	26
The role of orphan crops in the transition to nutritional quality-oriented crop improvement	26
Engineering conditional protein-protein interactions for dynamic cellular control	26
4D printing: Perspectives for the production of sustainable plastics for agriculture	26
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	26
Role of distal sites in enzyme engineering	26
Expanding the horizon of transient CAR T therapeutics using virus-free technology	26
Designing artificial pathways for improving chemical production	25
The new frontier in CHO cell line development: From random to targeted transgene integration technologies	25
Editorial Board	25
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	25
Current innovations in mRNA vaccines for targeting multidrug-resistant ESKAPE pathogens	24
Quantifying the impact of cell culture media on CHO cell growth and protein production	24
Editorial Board	24
Microbial transformations by sulfur bacteria can recover value from phosphogypsum: A global problem and a possible solution	24
The vast repertoire of carbohydrate oxidases: An overview	24
Insights to improve the plant nutrient transport by CRISPR/Cas system	24
In-depth analysis of biocatalysts by microfluidics: An emerging source of data for machine learning	24
The evolving art of creating genetic diversity: From directed evolution to synthetic biology	23
Challenges in developing cell culture media using machine learning	23
Trends in digital image processing of isolated microalgae by incorporating classification algorithm	23
Agrobacterium strains and strain improvement: Present and outlook	23
Decoding cellular mechanism of recombinant adeno-associated virus (rAAV) and engineering host-cell factories toward intensified viral vector manufacturing	23
Alternative biological sources for extracellular vesicles production and purification strategies for process scale-up	23
Surrogate broodstock to enhance biotechnology research and applications in aquaculture	23
Biocatalytic production of biolubricants: Strategies, problems and future trends	23
Advances in synthetic biology toolboxes paving the way for mechanistic understanding and strain engineering of gut commensal Bacteroides spp. and Clostridium spp.	22
Nanobubble technologies: Applications in therapy from molecular to cellular level	22
Reprint of: Advanced glycosidases as ingenious biosynthetic instruments	22
Towards a seamless product and process development workflow for recombinant proteins produced by plant molecular farming	22
Noble metal nanoparticles in agriculture: impacts on plants, associated microorganisms, and biotechnological practices	22
Editorial Board	22
A comprehensive assessment of Yarrowia lipolytica and its interactions with metals: Current updates and future prospective	22
The role of the proteosurfaceome and exoproteome in bacterial coaggregation	22
Editorial Board	22