Biotechnology Advances

Article	Citations
Using machine learning approaches for multi-omics data analysis: A review	377
Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies	350
Technologies for biological removal and recovery of nitrogen from wastewater	267
Enzyme co-immobilization: Always the biocatalyst designers' choice…or not?	161
Lignin nanoparticles enter the scene: A promising versatile green tool for multiple applications	161
Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms	156
Fermentation transforms the phenolic profiles and bioactivities of plant-based foods	155
Microbial electrosynthesis: Towards sustainable biorefineries for production of green chemicals from CO2 emissions	137
Microbial enzymes catalyzing keratin degradation: Classification, structure, function	135
Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices	133
Biopolymer-liposome hybrid systems for controlled delivery of bioactive compounds: Recent advances	132
Genetic engineering of microalgae for enhanced biorefinery capabilities	131
Biodegradation and up-cycling of polyurethanes: Progress, challenges, and prospects	124
Biotechnological production of astaxanthin from the microalga Haematococcus pluvialis	123
Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing	123
Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations	120
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	119
Purple phototrophic bacteria for resource recovery: Challenges and opportunities	119
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications	113
Potential of phytohormones as a strategy to improve microalgae productivity for biotechnological applications	112
Adaptive laboratory evolution principles and applications in industrial biotechnology	106
Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts	106
Biological production of medium-chain carboxylates through chain elongation: An overview	102
Prospects and development of algal-bacterial biotechnology in environmental management and protection	100
Flavoprotein monooxygenases: Versatile biocatalysts	94

Glycoside hydrolase family 18 chitinases: The known and the unknown	93
Ecofriendly biopolymers and composites: Preparation and their applications in water-treatment	91
Pseudomonas aeruginosa biofilms and their partners in crime	89
The Yin and Yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits	88
Strategies to manipulate the performance of aptamers in SELEX, post-SELEX and microenvironment	88
Archaea Biotechnology	86
The invisible life inside plants: Deciphering the riddles of endophytic bacterial diversity	86
Vero cell upstream bioprocess development for the production of viral vectors and vaccines	86
Bacterial cellulose and its potential for biomedical applications	84
Nanostructured antimicrobial peptides: The last push towards clinics	84
Antimicrobial properties and applications of metal nanoparticles biosynthesized by green methods	84
Prospects on bio-based 2,3-butanediol and acetoin production: Recent progress and advances	82
Anthocyanins, multi-functional natural products of industrial relevance: Recent biotechnological advances	81
Developing Aspergillus niger as a cell factory for food enzyme production	81
Metabolic engineering of Saccharomyces cerevisiae for the production of top value chemicals from biorefinery carbohydrates	80
Iron-assisted biological wastewater treatment: Synergistic effect between iron and microbes	80
Strategies employed in the design of antimicrobial peptides with enhanced proteolytic stability	75
Current and novel approaches to downstream processing of microalgae: A review	75
Boosting extracellular vesicle secretion	74
A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes	72
Molecularly imprinted polymers for the selective recognition of microorganisms	72
Microbial production of limonene and its derivatives: Achievements and perspectives	72
Production of plant natural products through engineered Yarrowia lipolytica	70
Inspired by nature: Microbial production, degradation and valorization of biodegradable bioplastics for life-cycle-engineered products	69
Acetate as a potential feedstock for the production of value-added chemicals: Metabolism and applications	69
Challenges in biodegradation of non-degradable thermoplastic waste: From environmental impact to operational readiness	67
Review of advances in the development of laccases for the valorization of lignin to enable the production of lignocellulosic biofuels and bioproducts	67
Computational design of enzymes for biotechnological applications	65
Stress response in Rhodococcus strains	64
Genetic engineering of microalgae for enhanced lipid production	64
Advances in the profiling of N6-methyladenosine (m6A) modifications	64
Microalgae with artificial intelligence: A digitalized perspective on genetics, systems and products	63
Amidase as a versatile tool in amide-bond cleavage: From molecular features to biotechnological applications	61
Biotechnological production of lipid and terpenoid from thraustochytrids	61
Recent trends in methane to bioproduct conversion by methanotrophs	61
Recent advancements and challenges in emerging applications of biochar-based catalysts	61
Understanding gradients in industrial bioreactors	61
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	61
Downstream processing technologies in the biocatalytic production of oligosaccharides	59
A navigation guide of synthetic biology tools for Pseudomonas putida	59
Self-assembling peptide-based hydrogels: Fabrication, properties, and applications	58
Biotechnological approaches for the production of natural colorants by Talaromyces/Penicillium: A review	58
Alternative sources and metabolic engineering of Taxol: Advances and future perspectives	56
Bio-synthesis of food additives and colorants-a growing trend in future food	56
Mechanical properties of single cells: Measurement methods and applications	56
Biofilm control by interfering with c-di-GMP metabolism and signaling	55
Production of polyunsaturated fatty acids by Schizochytrium (Aurantiochytrium) spp.	55
How can ethanol enhance direct interspecies electron transfer in anaerobic digestion?	55
On the verge of the market – Plant factories for the automated and standardized production of biopharmaceuticals	54
Biotechnological production and high potential of furan-based renewable monomers and polymers	54

Pathogens and predators impacting commercial production of microalgae and cyanobacteria	54
How does the Internet of Things (IoT) help in microalgae biorefinery?	54
Smart microalgae farming with internet-of-things for sustainable agriculture	54
Unlocking the secret of lignin-enzyme interactions: Recent advances in developing state-of-the-art analytical techniques	54
Review: Remotely controlled magneto-regulation of therapeutics from magnetoelastic gel matrices	53
Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: Recent advancements and current challenges	53
Can anaerobic digestion be a suitable end-of-life scenario for biodegradable plastics? A critical review of the current situation, hurdles, and challenges	53
Extremophilic lipases for industrial applications: A general review	52
Engineering whole-cell microbial biosensors: Design principles and applications in monitoring and treatment of heavy metals and organic pollutants	52
Hot spots-making directed evolution easier	51
Strategies for improving antimicrobial peptide production	51
Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories	51
Biopolymers production from microalgae and cyanobacteria cultivated in wastewater: Recent advances	51
Trends in the development of innovative nanobiocatalysts and their application in biocatalytic transformations	51
Biocatalytic oxidation of fatty alcohols into aldehydes for the flavors and fragrances industry	50
Algal metabolites: An inevitable substitute for antibiotics	50
A review on the progress, challenges and prospects in commercializing microalgal fucoxanthin	49
Genetic control of grain appearance quality in rice	49
Cyanobacteria and microalgae in supporting human habitation on Mars	48
What do we learn from enzyme behaviors in organic solvents? – Structural functionalization of ionic liquids for enzyme activation and stabilization	48
Plant synthetic biology for producing potent phyto-antimicrobials to combat antimicrobial resistance	48
From lignocellulose to plastics: Knowledge transfer on the degradation approaches by fungi	47
Ionic liquids for regulating biocatalytic process: Achievements and perspectives	47
From bumblebee to bioeconomy: Recent developments and perspectives for sophorolipid biosynthesis	47
Refactoring transcription factors for metabolic engineering	47
Recent advances in lipid metabolic engineering of oleaginous yeasts	46
Physical methods for controlling bacterial colonization on polymer surfaces	46
Waste-to-nutrition: a review of current and emerging conversion pathways	46
Microbial starch debranching enzymes: Developments and applications	46
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	45
Marine drugs: Biology, pipelines, current and future prospects for production	45
Engineer probiotic bifidobacteria for food and biomedical applications - Current status and future prospective	45
Biotransformation of dietary phytoestrogens by gut microbes: A review on bidirectional interaction between phytoestrogen metabolism and gut microbiota	45
Chemical modification of enzymes to improve biocatalytic performance	44
Mycolicibacterium cell factory for the production of steroid-based drug intermediates	44
Bacterial volatile organic compounds as biopesticides, growth promoters and plant-defense elicitors: Current understanding and future scope	44
Bacterial and enzymatic degradation of poly(cis-1,4-isoprene) rubber: Novel biotechnological applications	44
Electro-fermentation: Sustainable bioproductions steered by electricity	44
Expansion of human mesenchymal stem/stromal cells (hMSCs) in bioreactors using microcarriers: lessons learnt and what the future holds	44
Microbial-enabled green biosynthesis of nanomaterials: Current status and future prospects	43
Opportunities and challenges of the tag-assisted protein purification techniques: Applications in the pharmaceutical industry	43
Predicting drug-microbiome interactions with machine learning	42
Biotechnological applications of the non-conventional yeast Meyerozyma guilliermondii	41
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	41
Cellulose- and xylan-degrading yeasts: Enzymes, applications and biotechnological potential	40
Towards efficient enzymatic saccharification of pretreated lignocellulose: Enzyme inhibition by lignin-derived phenolics and recent trends in mitigation strategies	40
Marine macroalgae in a circular economy context: A comprehensive analysis focused on residual biomass	40
Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration	40
Lactic acid bacteria-derived α-glucans: From enzymatic synthesis to miscellaneous applications	40
Non-viral transfection technologies for next-generation therapeutic T cell engineering	39
Developing fungal heterologous expression platforms to explore and improve the production of natural products from fungal biodiversity	39
Models of microalgal cultivation for added-value products - A review	39
Insights on the emerging biotechnology of histidine-rich peptides	39
Bacterial membrane vesicle functions, laboratory methods, and applications	38
Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects	38
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	38
Comparative analysis of the chemical and biochemical synthesis of keto acids	37
Type II methanotrophs: A promising microbial cell-factory platform for bioconversion of methane to chemicals	37
Dehydrogenases of acetic acid bacteria	37
The tale of a versatile enzyme: Molecular insights into keratinase for its industrial dissemination	37
Biopolymers and their derivatives: Key components of advanced biomedical technologies	37
Rhodopseudomonas palustris: A biotechnology chassis	37
Agrobacterium strains and strain improvement: Present and outlook	37
From fungal secretomes to enzymes cocktails: The path forward to bioeconomy	37
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	37
Xylanases of glycoside hydrolase family 30 – An overview	37
Noble metal nanoparticles in agriculture: impacts on plants, associated microorganisms, and biotechnological practices	37
Surrogate broodstock to enhance biotechnology research and applications in aquaculture	37
Advances and perspectives on mass transfer and enzymatic hydrolysis in the enzyme-mediated lignocellulosic biorefinery: A review	36
Biofilm application in the microbial biochemicals production process	36
Microbial chassis engineering drives heterologous production of complex secondary metabolites	35
Advances in enzymatic oxyfunctionalization of aliphatic compounds	35
Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials	35
Bacillus licheniformis: The unexplored alternative for the anaerobic production of lipopeptide biosurfactants?	35
Insertions and deletions in protein evolution and engineering	35
Microbial inhibition and biosensing with multifunctional carbon dots: Progress and perspectives	34
Synthetic biology-inspired strategies and tools for engineering of microbial natural product biosynthetic pathways	34
Bionanomaterials based on protein self-assembly: Design and applications in biotechnology	34
Recent advances in fed-batch microscale bioreactor design	34
4D printing: Perspectives for the production of sustainable plastics for agriculture	34

Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	34
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	33
Agarose degradation for utilization: Enzymes, pathways, metabolic engineering methods and products	33
Photocatalyst-enzyme hybrid systems for light-driven biotransformation	32
Engineering of primary carbon metabolism in filamentous fungi	32
Computer-aided understanding and engineering of enzymatic selectivity	32
An overview and future prospects of sialic acids	32
Progress in biohythane production from microalgae-wastewater sludge co-digestion: An integrated biorefinery approach	32
Bacterial membrane transporter systems for aromatic compounds: Regulation, engineering, and biotechnological applications	32
Towards Green Nanoscience: From extraction to nanoformulation	32
The bacterial phylum Planctomycetes as novel source for bioactive small molecules	31
Cellular pathways of recombinant adeno-associated virus production for gene therapy	31
Genetic toolkits for engineering Rhodococcus species with versatile applications	31
Structured edible lipid-based particle systems for oral drug-delivery	31
Bacterial biofilms as platforms engineered for diverse applications	31
Engineering salt tolerance of photosynthetic cyanobacteria for seawater utilization	31
Factors affecting the quality of therapeutic proteins in recombinant Chinese hamster ovary cell culture	30
Heterologous expression of lytic polysaccharide monooxygenases (LPMOs)	30
Synthetic protein scaffolds for the colocalisation of co-acting enzymes	30
Recent advances in machine learning applications in metabolic engineering	30
Recent advances in human respiratory epithelium models for drug discovery	30
Design and biocatalytic applications of genetically fused multifunctional enzymes	30
Gum polysaccharide/nanometal hybrid biocomposites in cancer diagnosis and therapy	29
The evolving art of creating genetic diversity: From directed evolution to synthetic biology	29
Contribution of quorum sensing to virulence and antibiotic resistance in zoonotic bacteria	29
Advances in plant-derived C-glycosides: Phytochemistry, bioactivities, and biotechnological production	29
Immobilized fungal enzymes: Innovations and potential applications in biodegradation and biosynthesis	29
Kluyveromyces marxianus as a microbial cell factory for lignocellulosic biomass valorisation	29
Trends in digital image processing of isolated microalgae by incorporating classification algorithm	29
Recent advances in biocatalysis of nitrogen-containing heterocycles	29
Advances in synthetic biology tools paving the way for the biomanufacturing of unusual fatty acids using the Yarrowia lipolytica chassis	28
Bacterial sialyltransferases and their use in biocatalytic cascades for sialo-oligosaccharide production	28
CRISPR-based enrichment strategies for targeted sequencing	28
Bacterial protein acetylation and its role in cellular physiology and metabolic regulation	28
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	28
Bioprospecting for bioactive compounds in microalgae: Antimicrobial compounds	28
Functionalized chitosan for cancer nano drug delivery	28
Adaptive innovation of green plants by horizontal gene transfer	28
Feedback regulation and coordination of the main metabolism for bacterial growth and metabolic engineering for amino acid fermentation	28
Halocins, natural antimicrobials of Archaea: Exotic or special or both?	28
Challenges and progress towards industrial recombinant protein production in yeasts: A review	27
Insights to improve the plant nutrient transport by CRISPR/Cas system	27
Role of distal sites in enzyme engineering	27
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	27
Medicinal plants used in the treatment of tuberculosis - Ethnobotanical and ethnopharmacological approaches	27
Advanced glycosidases as ingenious biosynthetic instruments	27
Recent advances in enzyme-enhanced immunosensors	26
The function of UDP-glycosyltransferases in plants and their possible use in crop protection	26
Plants arabinogalactans: From structures to physico-chemical and biological properties	26
New trends in biotechnological applications of photosynthetic microorganisms	26
Molecular mechanism of ethanol-H2 co-production fermentation in anaerobic acidogenesis: Challenges and perspectives	26
The vast repertoire of carbohydrate oxidases: An overview	26
IgG glycans in health and disease: Prediction, intervention, prognosis, and therapy	26
Ionic liquids as protein stabilizers for biological and biomedical applications: A review	26
Resveratrol and cyclodextrins, an easy alliance: Applications in nanomedicine, green chemistry and biotechnology	26
Improving microalgae for biotechnology — From genetics to synthetic biology – Moving forward but not there yet	26
Employment of the CRISPR/Cas9 system to improve cellulase production in Trichoderma reesei	26
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	25
Nanoantibiotics to fight multidrug resistant infections by Gram-positive bacteria: hope or reality?	25
Alternative biological sources for extracellular vesicles production and purification strategies for process scale-up	25
Recent advances in tuning the expression and regulation of genes for constructing microbial cell factories	25
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	25
The signaling pathways involved in metabolic regulation and stress responses of the yeast-like fungi Aureobasidium spp.	25
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	24
Bacterial conversion routes for lignin valorization	24
Mimicking natural strategies to create multi-environment enzymatic reactors: From natural cell compartments to artificial polyelectrolyte reactors	24
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	23
Genome edited wheat- current advances for the second green revolution	23
Insect odorant receptor-based biosensors: Current status and prospects	23
Engineering Staphylococcal Protein A for high-throughput affinity purification of monoclonal antibodies	23
Insights into the source, mechanism and biotechnological applications of hyaluronidases	23
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	23
Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria	22
Heterologous production of cellulose- and starch-degrading hydrolases to expand Saccharomyces cerevisiae substrate utilization: Lessons learnt	22
Green Biorefinery systems for the production of climate-smart sustainable products from grasses, legumes and green crop residues	22
Application of microbial 3-ketosteroid Δ1-dehydrogenases in biotechnology	22
Exploration of space to achieve scientific breakthroughs	22
Metabolic engineering of microbes for monoterpenoid production	22
Improvement of the enzymatic detoxification activity towards mycotoxins through structure-based engineering	22
The isolation of lignin with native-like structure	22
Metabolic engineering and synthetic biology strategies for producing high-value natural pigments in Microalgae	22
Bioelectrocatalysis for CO2 reduction: recent advances and challenges to develop a sustainable system for CO2 utilization	22
Aberrant protein glycosylation: Implications on diagnosis and Immunotherapy	21
Plant-made immunotoxin building blocks: A roadmap for producing therapeutic antibody-toxin fusions	21
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	21
Non-conventional yeast strains: Unexploited resources for effective commercialization of second generation bioethanol	21
Microbial transformations by sulfur bacteria can recover value from phosphogypsum: A global problem and a possible solution	21
Manufacturing of bacteriophages for therapeutic applications	21
Refolding in the modern biopharmaceutical industry	21
Bio-valorization of C1 gaseous substrates into bioalcohols: Potentials and challenges in reducing carbon emissions	21
Metabolic and biotransformation effects on dietary glucosinolates, their bioavailability, catabolism and biological effects in different organisms	21
Current and emerging applications of saccharide-modified chitosan: a critical review	20
Recent progress on health effects and biosynthesis of two key sialylated human milk oligosaccharides, 3′-sialyllactose and 6′-sialyllactose	20
Advances on microfluidic paper-based electroanalytical devices	20
Current status, challenges and prospects for lignin valorization by using Rhodococcus sp.	20