Biotechnology Advances

Article	Citations
Editorial Board	314
Editorial Board	178
Evolving perspectives on lutein production from microalgae - A focus on productivity and heterotrophic culture	168
Use of additives to improve collective biogas plant performances: A comprehensive review	163
Targeted analysis of metagenomes: divide and conquer	152
Decoding microbial interactions: Interaction networks and regulatory strategies for medium-chain fatty acid biosynthesis through anaerobic chain elongation	151
Anaerobic digestion integrated with microbial electrolysis cell to enhance biogas production and upgrading in situ	150
Methanol metabolism in synthetic methylotrophic microorganisms	139
Collagen processing with mesoscale aggregates as templates and building blocks	129
Biotechnological production and emerging applications of betalains: A review	125
Advancements of astaxanthin production in Haematococcus pluvialis: Update insight and way forward	124
Biotechnological advances in key regulatory genes of phenylpropanoid and terpenoid biosynthesis pathways in Panax ginseng: Current insights and future prospects	120
New perspectives into Gluconobacter-catalysed biotransformations	118
Development of isopentenyl phosphate kinases and their application in terpenoid biosynthesis	115
Opportunities for visual techniques to determine characteristics and limitations of electro-active biofilms	113
Benzylisoquinoline alkaloid production: Moving from crop farming to chemical and biosynthesis	112
Pickering emulsion biocatalysis: Bridging interfacial design with enzymatic reactions	110
Potential for microbial plastic degradation via assimilation of non-carbon moieties in additives	110
Machine learning methods for small data and upstream bioprocessing applications: A comprehensive review	108
Accessing hidden microbial biosynthetic potential from underexplored sources for novel drug discovery	107
Recent advances and perspectives on production of value-added organic acids through metabolic engineering	107
Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives	106
Biosensors in microalgae: A roadmap for new opportunities in synthetic biology and biotechnology	103
Chiral helical scaffolds: Unlocking their potential in biomolecular interactions and biomedical applications	102
Microbial computing: Review and Perspectives	101

Phosphorylated polysaccharides: Applications, natural abundance, and new-to-nature structures generated by chemical and enzymatic functionalisation	100
Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications	100
Editorial Board	98
Plant cell wall biosynthesis: Immune signaling, genome editing, and physiological implications for biomass valorization	97
Biological valorization of lignin to flavonoids	97
Advances in gene editing-led route for hybrid breeding in crops	92
Droplet microfluidics for single-cell analysis: From improved cell encapsulation and sorting technologies to innovative applications	92
Advances and challenges in enzymatic rubber degradation: Exploring genetic, molecular, and biotechnological aspects	90
Methanotrophs as a reservoir for bioactive secondary metabolites: Pitfalls, insights and promises	89
Emerging research insights and future perspectives on the advancement of eccDNA: A comprehensive review	89
The path to biotechnological singularity: Current breakthroughs and outlook	86
Recent advances in machine learning applications in metabolic engineering	84
Biotechnological applications of purine and pyrimidine deaminases	82
Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects	80
Electrode functional microorganisms in bioelectrochemical systems and its regulation: A review	80
Growth-coupled high throughput selection for directed enzyme evolution	80
Computational methods for spatial multi-omics integration	78
Ex-situ biomethanation for CO2 valorization: State of the art, recent advances, challenges, and future prospective	78
Alpha-ketoacid decarboxylases: Diversity, structures, reaction mechanisms, and applications for biomanufacturing of platform chemicals and fuels	77
Haloferax and the Haloferacaceae: Potential role in bioindustry	77
Polyethylene biodegradation: A multifaceted approach	76
Protease engineering: Approaches, tools, and emerging trends	75
Genome-scale metabolic network models for industrial microorganisms metabolic engineering: Current advances and future prospects	74
Advances in transcription factor-based biosensors for natural product biosynthesis: Optimization, emerging technologies, and future prospects	74
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements	73
Bioinspired nanoplatforms for human-machine interfaces: Recent progress in materials and device applications	73
Polyhydroxyalkanoates synthesis by halophiles and thermophiles: towards sustainable production of microbial bioplastics	73
Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions	73
Biocatalytic oxyfunctionalization of unsaturated fatty acids to oxygenated chemicals via hydroxy fatty acids	73
Improving recombinant protein production in CHO cells using the CRISPR-Cas system	73
Emerging advances in glycoengineering of carbohydrates/glycans and their industrial applications	71
Strategies for overcoming the limitations of enzymatic carbon dioxide reduction	71
Unlocking the bacterial domain for industrial biotechnology applications using universal parts and tools	70
Rutinosidase and other diglycosidases: Rising stars in biotechnology	69
Research progress of engineering microbial cell factories for pigment production	69
Expanding sugar alcohol industry: Microbial production of sugar alcohols and associated chemocatalytic derivatives	69
Editorial Board	69
Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories	68
Advances in in vitro cultivation techniques for comprehensive analysis of human gut microbiome	68
Fusarium as a promising fungal genus with potential application in bioremediation for pollutants mitigation: A review	67
Influence of elevated pressure and pressurized fluids on microenvironment and activity of enzymes	67
Optimizing prime editing: Advances in efficiency enhancement	67
Utilization of gene manipulation system for advancing the biotechnological potential of halophiles: A review	67
Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation	66
Loop engineering in enzymes from structure to function: Mechanisms, methodologies, and engineering strategies	65
The fungal cure: Harnessing mycelial approach as sustainable green solution for industrial waste treatment	64
Recent advances in research for potential utilization of unexplored lichen metabolites	63
Engineering oleaginous red yeasts as versatile chassis for the production of oleochemicals and valuable compounds: Current advances and perspectives	62
From lignocellulosic biomass to single cell oil for sustainable biomanufacturing: Current advances and prospects	62
Editorial Board	61

Resource allocation in mammalian systems	61
Engineering membrane architecture for biotechnological applications	59
Biological conversion of carbon monoxide and hydrogen by anaerobic culture: Prospect of anaerobic digestion and thermochemical processes combination	58
Is it possible to shape the microalgal biomass composition with operational parameters for target compound accumulation?	58
Construction and applications of methanol bio-converting cell factories	58
Machine learning in predictive biocatalysis: A comparative review of methods and applications	58
Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications	58
Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities	57
UGT708 glycosyltransferases: Nature's architects of C-glycosides	57
Microalgal bioengineering for futuristic applications in synthetic and space biology	56
Harnessing the biomolecular mechanisms of marine biomineralisation for carbon sequestration	56
Recent advances in engineering synthetic biomolecular condensates	56
Cytoskeleton as a roadmap navigating rhizobia to establish symbiotic root nodulation in legumes	55
C-type lectin-(like) fold – Protein-protein interaction patterns and utilization	54
Synthetic biology approaches to improve tolerance of inhibitors in lignocellulosic hydrolysates	53
Genetic engineering of bacteriophages: Key concepts, strategies, and applications	53
Smart culture medium optimization for recombinant protein production: Experimental, modeling, and AI/ML-driven strategies	52
Detection, production, modification, and application of arylsulfatases	52
One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins	51
Corrigendum to “Recent advances in enzyme-enhanced immunosensors” [Biotechnology Advances 53 (2021) 107867].	51
Microbial collaboration in bioelectrochemical systems: Mechanisms and advances towards sustainable biobased products	50
A reflection on the improvement of Chinese hamster ovary cell-based bioprocesses through advances in proteomic techniques	50
Editorial Board	49
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics	49
Flavin reductases in two-component systems: Mechanistic insights, structural classification, and biotechnological advances	49
Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies	49
From traditional to AI-driven: The evolution of intelligent enzyme engineering for biocatalysis	48
Corrigendum to ‘Expanding biotechnological applications of Yarrowia lipolytica: Key advances in the past decade’ [Biotechnol. Adv. 88 (2026) 108835]	48
Enzyme miniaturization: Revolutionizing future biocatalysts	47
Principles of antibodies with ultralong complementarity-determining regions and picobodies	47
Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review	47
Optimizing microbial networks through metabolic bypasses	46
Abiotic stress as a dynamic strategy for enhancing high value phytochemicals in microalgae: Critical insights, challenges and future prospects	46
Advance computational tools for multiomics data learning	46
Recent advances in chemoenzymatic synthesis of human ABO blood group antigens	46
Advances in multi-enzyme co-localization strategies for the construction of microbial cell factory	46
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances	45
Interpretable and explainable predictive machine learning models for data-driven protein engineering	45
Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products	45
The technology, opportunities, and challenges of Synthetic Biological Intelligence	45
A guide to the use of bioassays in exploration of natural resources	44
Brews, fuels, and opioids: Expanding the yeast Ehrlich pathway for chemical and pharmaceutical manufacturing	43
Portable biosensors for rapid on-site determination of cannabinoids in cannabis, a review	43
Synthetic biology of Fusarium for the sustainable production of valuable bioproducts	43
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications	43
Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects	43
Functionalized chitosan for cancer nano drug delivery	43
Geometric deep learning assists protein engineering. Opportunities and Challenges	43
Trait drift in microalgae and applications for strain improvement	42
Bioactive metabolites and extracellular vesicles from the marine chlorophyte genus Tetraselmis: Review	42
Rumen microbes, enzymes, metabolisms, and application in lignocellulosic waste conversion - A comprehensive review	42
Enzymatic C N bond formation and cleavage reaction: Advances and perspectives	42
Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii	42
Fungal bioconversion of lignin-derived aromatics: Pathways, enzymes, and biotechnological potential	41
Solar-powered quantum dot-biocatalyst biohybrids for semi-artificial photosynthesis: Advances in interfacial design and energy-mass transfer optimisation	41
Bacterioferritin nanocage: Structure, biological function, catalytic mechanism, self-assembly and potential applications	41
Adaptability and resiliency of Lepidoptera and their cells in culture - linking evolutionary biology to biotechnological advantage	41
Biofilm control by interfering with c-di-GMP metabolism and signaling	41
The stressing point: how plants respond to environmental stimuli	41
The Gompertz model and its applications in microbial growth and bioproduction kinetics: Past, present and future	41
Microbial transport systems of organic sulfur compounds: Diversity and implications for biocatalysis, healthcare, and environmental biotechnology	41
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates	41
Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms	41
Optogenetic technologies in translational cancer research	40
Design and application of microfluidics in aptamer SELEX and Aptasensors	40
The isolation of lignin with native-like structure	40
Recent advances in phyto-combined remediation of heavy metal pollution in soil	40
Microbial gas fermentation technology for sustainable food protein production	39
Recent advances in the identification of biosynthetic genes and gene clusters of the polyketide-derived pathways for anthraquinone biosynthesis and biotechnological applications	39
Strategies for the biological production of ectoine by using different chassis strains	39
Advancements in nanomaterial-based aptasensors for the detection of emerging organic pollutants in environmental and biological samples	39
Plant glycoengineering for designing next-generation vaccines and therapeutic proteins	39
Editorial Board	39
Current status, and the developments of hosts and expression systems for the production of recombinant human cytokines	39
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli	38
Biopreservation of beer: Potential and constraints	38
Recent advances in microbial electron transfer-driven bio-reductive immobilization of heavy metals: Mechanisms, enhancement strategies, and perspectives	38
Protein representations: Encoding biological information for machine learning in biocatalysis	38
Recent advances and discoveries of microbial-based glycolipids: Prospective alternative for remediation activities	38
Whole-organism phenotypic screening methods used in early-phase anthelmintic drug discovery	38

Challenges and progress towards industrial recombinant protein production in yeasts: A review	38
Controlled processivity in glycosyltransferases: A way to expand the enzymatic toolbox	37
Cupriavidus necator as a platform for polyhydroxyalkanoate production: An overview of strains, metabolism, and modeling approaches	37
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications	37
Ferritin nanocage based delivery vehicles: From single-, co- to compartmentalized- encapsulation of bioactive or nutraceutical compounds	37
Recent advances in microbial production of diamines, aminocarboxylic acids, and diacids as potential platform chemicals and bio-based polyamides monomers	37
CRISPR-Cas13 technology portfolio and alliance with other genetic tools	37
Influence of glycosylation on the immunogenicity and antigenicity of viral immunogens	37
Xylanases targeting unusual glycosidic bonds: Unlocking prebiotic xylooligosaccharides from macroalgae	37
Yeast as C1 cell factory: Transforming methanol and Formate into high-value compounds	37
Genetic engineering of Saccharomyces boulardii: Tools, strategies and advances for enhanced probiotic and therapeutic applications	37
Comparative evaluation of large language models for biotechnology review writing	37
Turning waste into treasure: A new direction for low-cost production of lipid chemicals from Thraustochytrids	36
The evolving landscape of recombinant adeno-associated virus (rAAV) manufacturing: Engineering vector plasmids for upstream production	36
Applications of bio-capacitance to cell culture manufacturing	36
Engineered transcription factor-binding diversed functional nucleic acid-based synthetic biosensor	36
Advancements of immunoassay technology based on nanobodies	35
Membranes: The only chance for the mRNA tortoise to win, economically	35
Using oils and fats to replace sugars as feedstocks for biomanufacturing: Challenges and opportunities for the yeast Yarrowia lipolytica	35
Selection of aptamers targeting small molecules by capillary electrophoresis: Advances, challenges, and prospects	35
HEK-omics: The promise of omics to optimize HEK293 for recombinant adeno-associated virus (rAAV) gene therapy manufacturing	35
Advances of computational protein design: Principles, strategies and applications in nutrition and health	35
Harnessing spatial transcriptomics to understand host-parasite interactions in plants and animals	35
Circular RNAs in microalgae: Uncovering their biological significance	35
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger	34
Bioinformatics assisted construction of the link between biosynthetic gene clusters and secondary metabolites in fungi	34
Refactoring transcription factors for metabolic engineering	34
Recent advances in the covalent-bond-based peptide tagging systems and their applications	34
Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics	34
The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction	33
Fluorescence coupling strategies in fluorescence-activated droplet sorting (FADS) for ultrahigh-throughput screening of enzymes, metabolites, and antibodies	33
Computational advances in biosynthetic gene cluster discovery and prediction	33
Advances in production and application technologies of minicells: A review	33
Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil healt	33
Designer bacterial cell factories for improved production of commercially valuable non-ribosomal peptides	33
From natural to synthetic: Promoter engineering in yeast expression systems	33
Exploitation of lignocellulosic-based biomass biorefinery: A critical review of renewable bioresource, sustainability and economic views	32
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0	32
Grafting of proteins onto polymeric surfaces: A synthesis and characterization challenge	32
Cellular variability as a driver for bioprocess innovation and optimization	32
Editorial Board	32
Utilizing stimuli-responsive nanoparticles to deliver and enhance the anti-tumor effects of bilirubin	32
Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing	32
Revolutionizing agroindustry: Towards the industrial application of antimicrobial peptides against pathogens and pests	32
Understanding low-phosphate stress responses in plants: Opportunities for genome editing to improve phosphorous use efficiency (PUE)	31
Implications of glycosylation for the development of selected cytokines and their derivatives for medical use	31
Vanadium-dependent haloperoxidases: Recent advances and perspectives	31
Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology	31
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy	31
From modular engineering to practical applications: Advances in GPCR-based yeast biosensors	31
Lipopeptides development in cosmetics and pharmaceutical applications: A comprehensive review	31
Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions	30
Revolutionizing keratinase science: Biocatalytic advances, sustainable innovation, and industrial perspectives	30
Epigenetic therapy for leukemias: a comprehensive review from biological markers to practice	30
Decoding bioprocesses with transcriptomics: current status and future potential	30
Yeast surface display technology: Mechanisms, applications, and perspectives	30
Design and biocatalytic applications of genetically fused multifunctional enzymes	30
Molecular biology: Fantastic toolkits to improve knowledge and application of acetic acid bacteria	30
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms	30
Viral clearance in biopharmaceutical manufacturing: Current strategies, challenges, and future directions	30
Strategies for improving antimicrobial peptide production	30
Progress in cyclodextrins as important molecules regulating catalytic processes of glycoside hydrolases	30
Has the time finally come for green oleochemicals and biodiesel production using large-scale enzyme technologies? Current status and new developments	29
Glycobiohydrolases at the forefront of lignocellulose saccharification: A review on substrate specificities and structure-function properties	29
Advances in biosensors for microbial biosynthesis of amino acids and their derivatives	29
Deciphering mechanisms of production of natural compounds using inducer-producer microbial consortia	29
Optimization strategies for CO2 biological fixation	28
Biomass directional pyrolysis based on element economy to produce high-quality fuels, chemicals, carbon materials – A review	28
From formate oxidation to CO₂ reduction: The role of formate dehydrogenase in sustainable carbon utilization	28
Methods of in vitro study of galectin-glycomaterial interaction	28
Advances in the synthesis of three typical tetraterpenoids including β-carotene, lycopene and astaxanthin	28
Molecular imprinting technology for biomedical applications	28
Biopolymers and their derivatives: Key components of advanced biomedical technologies	28
Strategies for Automated Enzymatic Glycan Synthesis (AEGS)	27
Editorial Board	27
Host cell protein-mediated adjuvanticity and immunogenicity risks of biotherapeutics	27
Chimeric enzymes in the pulp and paper making industry: Current developments	26
Editorial Board	26
Cell-culture process optimization via model-based predictions of metabolism and protein glycosylation	26
Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances	26
Role of distal sites in enzyme engineering	26
Editorial Board	26
Nucleic acid-responsive smart systems for controlled cargo delivery	26
Baculovirus dual-phase infection strategy and biotechnological applications: A structural and regulatory review	26
Next generation probiotics: Engineering live biotherapeutics	26
Engineering lignin pathway, plant cell wall modification, and genome editing for advanced renewable bioenergy and material applications	26
Reporter systems in actinomycetes: Versatile tools for natural product discovery and production	26
Process simulation and evaluation of scaled-up biocatalytic systems: Advances, challenges, and future prospects	26
Antimicrobial peptides: Sustainable application informed by evolutionary constraints	25
A review on the expanding biotechnological frontier of Pedobacter	25
Biodegradation of polyethylene and polystyrene: From microbial deterioration to enzyme discovery	25
Production and characterization of starch-lignin based materials: A review	25
Biophotovoltaics: Recent advances and perspectives	25
Engineering status of protein for improving microbial cell factories	25
Engineering catalytically promiscuous enzymes to serve new functions	25