Atla-Alternatives to Laboratory Animals

Article	Citations
Extension of the Virtual Cell Based Assay from a 2-D to a 3-D Cell Culture Model	115
Spotlight on Three Rs Progress	40
Spotlight on Three Rs Progress	38
The Safety Assessment of Cosmetic Perfumes by Using In Chemico and In Vitro Methods in Combination with GC-MS/MS Analysis	35
The Use of Human Tissues for Research: What Investigators Need to Know	23
Conference Diary	23
Combinatorial induction with valproic acid and fibroblast growth factor-9 enhances neural differentiation and cell fate commitment in adipose tissue-derived mesenchymal stem cells	16
Resources Round-up	16
The Development of an Animal Product-free, Precision-cut Lung Slice Cryopreservation and Post-thaw Culture Method	16
Toxicological Profiling of Potential Shikimate Kinase Inhibitors Against Mycobacterium tuberculosis	16
Editorial	12
Dr Manfred Liebsch: Former Head of ZEBET and ATLA Editorial Board Member	11
Resources Round-up	11
Development of Sensitive In Vitro Protocols for the Biocompatibility Testing of Medical Devices and Pharmaceuticals Intended for Contact with the Eyes: Acute Irritation and Phototoxicity Assess	11
Conference Diary	10
A History of the Development of Alternatives to Animals in Research and Testing	10
Report of the First ONTOX Stakeholder Network Meeting: Digging Under the Surface of ONTOX Together With the Stakeholders	9
Brazilian National Network of Alternative Methods (RENAMA) 10th Anniversary: Meeting of the Associated Laboratories, May 2022	9
Editorial	9
In Silico Prediction of Human Clinical Pharmacokinetics with ANDROMEDA by Prosilico: Predictions for an Established Benchmarking Data Set, a Modern Small Drug Data Set, and a Comparison with La	9
The Rise of Three Rs Centres and Platforms in Europe*	8
The 19th FRAME Annual Lecture, November 2022: Safer Chemicals and Sustainable Innovation Will Be Achieved by Regulatory Use of Modern Safety Science, Not by More Animal Testing	7
Editorial	7
2021 Michael Balls Award	7
Progress and Remaining Opportunities to Increase the Use of Animal-free Antibodies in the USA	7

Spotlight on Three Rs Progress	7
Editorial	7
Fenugreek Seed Extract Regulates Human Umbilical Vein Endothelial Cell Angiogenesis and Proliferation via the PI3K/Akt/Cyclin D1 Pathway	6
Addressing Animal Welfare Issues in Fetal Blood Collection for Fetal Bovine Serum Production	6
Thanks to Reviewers	5
The Numbers of Animals Used in Mexico for Scientific and Educational Purposes	5
Resources Round-up	5
The impossible made possible through the use of New Approach Methodologies: Dermal safety assessment of potential insect repellent or insecticidal active ingredients using a high-throughput approach t	5
Editorial	5
Reviewing Current Guidance for the ‘R’ of Replacement and Rethinking it with the ‘Replacement Checklist’	5
A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of Bothrops jararaca Venom and Antivenom: An Intralaboratory Pre-validation Study	5
Editorial	4
Optimisation of a Method for the Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells Toward Renal Epithelial-like Cells	4
Case Studies Exemplifying the Transition to Animal Component-free Cell Culture	4
Book Review: Lab dog: A beagle and his human investigate the surprising world of animal research Melanie D.G. Kaplan (2025). Lab Dog: A Beagle and His Human Investigate the Surprising World of Animal	4
Development of Two Korean IACUC Guidance Documents to Foster Implementation of the Three Rs	4
Editorial	4
Spotlight on Three Rs Progress	4
Editorial	4
Corrigendum to Case Studies Exemplifying the Transition to Animal Component-Free Cell Culture	4
Resources Round-up	4
Editorial	4
Accelerating Animal Replacement: How Universities Can Lead — Results of a One-Day Expert Workshop in Zurich, Switzerland	4
Conference Diary	3
The Application of New Approach Methodologies in Respiratory Disease Research: Their Role in Improving Translational Medicine from Bench to Bedside	3
Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Second International Conference on 3Rs Research and Progress, Hyderabad, 2021	3
Determining the pathogenicity of uropathogenic Escherichia coli strains in the Galleria mellonella larvae model	3
A New ATLA Associate Editor	3
Spotlight on Three Rs Progress	3
Editorial	3
Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)	3
Editorial	3
Lessons for the Future of NAMs from History, Philosophy and Social Studies of Science	3
The Use of 3-D Models of Echocardiographic Imaging Planes for Teaching Echocardiography Techniques for Use in Dogs and Cats	3
The Current Status and Work of Three Rs Centres and Platforms in Europe*	3
Transparency in Non-Technical Project Summaries to Promote the Three Rs in Respiratory Disease Research	2
Resources Round-up	2
An Evaluation of the Replacement of Animal-derived Biomaterials in Human Primary Cell Culture	2
Modelling the Sorafenib-resistant Liver Cancer Microenvironment by Using 3-D Spheroids	2
ATLA Best Poster Award	2
Editorial	2
Spotlight on Three Rs Progress	2
Use of the Allium cepa Model to Assess the Cytogenotoxicity of Luffariella herdmani Marine Sponge Extract	2
Resources Round-up	1
Editorial	1
Resources Round-up	1
Implementing the EURL ECVAM Recommendation on Non-Animal-Derived Antibodies in One EU Member State — Denmark	1
Evaluating the translational value of preclinical models: Available tools and frameworks, challenges and strategies	1
Alternatives to Laboratory Animals: Trends in Replacement and the Three Rs	1
Conference Diary	1

A Comparison of Mesenchymal Stem Cell-derived Hepatocyte-like Cells and HepG2 Cells for Use in Drug-Induced Liver Injury Studies	1
Conference Diary	1
Barriers to the Use of Recombinant Bacterial Endotoxins Test Methods in Parenteral Drug, Vaccine and Device Safety Testing	1
An Accessible Nephrectomy and Cystotomy Simulator for Use in Teaching Small Animal Surgery	1
Conference Diary	1
Editorial	1
Resources Round-up	1
Conference Diary	1
Conference Diary	1
Resources Round-up	1
The Evolution of Regulatory Toxicology: Where is the Gardener?	1
Conference Diary	1
Spotlight on Three Rs Progress	1
Journeying Through Journals: The Publishing Process and How to Maximise Research Impact	1
Resources Round-up	1
Announcing the new ATLA Associate Editors	1
Corrigendum to Report of the First ONTOX Hackathon: Hack to Save Lives and Avoid Animal Suffering. The Use of Artificial Intelligence in Toxicology — A Potential Driver for Reducing/Replacing Laborato	1
Assessment of a Low-cost Canine Uterine Simulator as a Tool for Teaching the Ovariohysterectomy Technique to Veterinary Students	1
Resources Round-up	1
Biobank Personnel — The Key to its Success	1
The Use of Simulators for Teaching Practical Clinical Skills to Veterinary Students — A Review	1
The Effects of Urolithin B and Auraptene on Quinolinic Acid-induced Toxicity in the SH-SY5Y Neuroblastoma Cell Line	1
Spotlight on Three Rs Progress	1
The VEGAHUB Platform: The Philosophy and the Tools	1
Conference Diary	1
Editorial	0
Poor Translatability of Biomedical Research Using Animals — A Narrative Review	0
Resources Round-up	0
Editorial	0
Thanks to Reviewers	0
A Low-fidelity Simulator for the Development of Vascular Ligation Skills	0
Resources Round-up	0
Ciliary Beat Frequency: Proceedings and Recommendations from a Multi-laboratory Ring Trial Using 3-D Reconstituted Human Airway Epithelium to Model Mucociliary Clearance	0
Xeno-free alternatives to the use of fetal bovine serum in head and neck cancer explant culture	0
Spotlight on Three Rs Progress	0
Permutation Tests Are a Useful Alternative Approach for Statistical Hypothesis Testing in Small Sample Sizes	0
Report of the First ONTOX Hackathon: Hack to Save Lives and Avoid Animal Suffering. The Use of Artificial Intelligence in Toxicology — A Potential Driver for Reducing/Replacing Laboratory Animals in t	0
Recent ATLA Awards	0
The Prevalence of Reported Gavage Incidents in Repeated Dose Toxicity Studies Involving Rats Conducted Between 2004–2023	0
Spotlight on Three Rs Progress	0
Conference Diary	0
Charting a new path, replacing animals in biomedical research	0
Conference Diary	0
Conference Diary	0
Resources Round-up	0
The Perceptions of Students and Lecturers on the Use of Animals in Biomedical Science Undergraduate Education in Brazil	0
Spotlight on Three Rs Progress	0
An Evaluation of Different Types of Peptone as Partial Substitutes for Animal-derived Serum in Vero Cell Culture	0
Message from FRAME	0
Book Review: Zebrafish Model for Biomedical Research	0
Spotlight on Three Rs Progress	0
The Capability Maturity Model as a Measure of Culture of Care in Laboratory Animal Science	0
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an In Vitro Model Based on Differentiated PC12 Cells	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Development of a 3D-printed canine cardiac disease model for interventional procedure training	0
Cardiac Transcription Regulators Differentiate Human Umbilical Cord Mesenchymal Stem Cells into Cardiac Cells	0
A Short History of the Consideration of Sex Differences in Biomedical Research — Lessons for the In Vitro Community from Animal Models and Human Clinical Trials	0
Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022	0
A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
Editorial	0
In Silico Prediction of Eye Irritation Using Hansen Solubility Parameters and Predicted pKa Values	0
Editorial	0
Celebrating Professor Horst Spielmann’s 80th Birthday	0
Conference Diary	0
Cigarette Smoke Constituents and Nicotine Differentially Affect Cytokine Production by Human Macrophages Stimulated by TLR Ligands In Vitro: Considerations for a Standardised Protocol	0
Spotlight on Three Rs Progress	0
Conference Diary	0
Resources Round-up	0
Spotlight on Three Rs Progress	0
In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava	0
Chemicals 2.0 and Why We Need to Bypass the Gold Standard in Regulatory Toxicology	0
Conference Diary	0
2023 Michael Balls Award	0
Conference Diary	0
Conference Diary	0
Chitosan–Calcium Aluminate as a Cell-homing Scaffold: Its Bioactivity Testing in a Microphysiological Dental Pulp Platform	0

Breaking down the barriers to animal-free research	0
Mesoporous bioactive glass nanoparticles exhibit intrinsic angiogenic potential in the chorioallantoic membrane assay, without the addition of exogenous cells	0
Thanks to Reviewers	0
Conference Diary	0
The Use of an In Chemico Digestibility Assay to Reduce the In Vivo Fish Bioaccumulation Testing of Nanomaterials	0
Conference Diary	0
The Use of MPS in Three Rs and Regulatory Applications: Perspectives From Developers on Stakeholder Responsibilities	0
In Silico Phototoxicity Prediction of Drugs and Chemicals by using Derek Nexus and QSAR Toolbox	0
Conference Diary	0
Resources Round-up	0
Expression of Concern: “Fenugreek Seed Extract Regulates Human Umbilical Vein Endothelial Cell Angiogenesis and Proliferation via the PI3K/Akt/Cyclin D1 Pathway”	0
The Istanbul Workshop organised by the ‘IMPROVE’ COST Action Ethics Crossover Group	0
Determination of the Microinjected Cytosolic Concentration by Using Mathematical Models of Fluid Dynamics	0
Resources Round-up	0
Rethinking the Three Rs: Growing Momentum Toward a New Gold Standard in Australian Research	0
Evaluating the Impact of Hair Care Product Exposure on Hair Follicle and Scalp Health	0
The 3Ranker: An AI-based Algorithm for Finding Non-animal Alternative Methods	0
The Recombinant Antibodies & Mimetics Database: Redefining the future of antibody use in science	0
A systematic review of the role of laboratory animal veterinarians in promoting One Welfare	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
A History of Regulatory Animal Testing: What Can We Learn?	0
Key Challenges for In Vitro Testing of Tobacco Products for Regulatory Applications: Recommendations for the In Vitro Mouse Lymphoma Assay	0
Optimisation of Conditions for the Formation of Spheroids of Head and Neck Squamous Cell Carcinoma Cell Lines for Use as Animal Alternatives	0
A History of the Development of Alternatives to Animals in Research and Testing	0
Educators’ Views on the Use of Dissection and Dissection Alternatives in American Biology Classrooms	0
Resources Round-up	0
Conference Diary	0
Resources Round-up	0
Biosensor-based approaches: Exploring hapten – protein interactions for the prediction of skin sensitisation	0
Acute Toxicity Assays with the Artemia salina Model: Assessment of Variables	0
Dr Richard Clothier: FRAME Trustee, Founding Director of the FRAME Laboratory and ATLA Editorial Board Member	0
Adapting to Changes in Publishing When Searching for Alternatives and Reporting on Animal Research: A Librarian’s Perspective	0
How to Foster ‘New Approach Methodology’ Toxicologists	0
Spotlight on Three Rs Progress	0
Conference Diary	0
Development of an Accessible Gene Expression Bioinformatics Pipeline to Study Driver Mutations of Colorectal Cancer	0
Editorial	0
Conference Diary	0
An Approach to Setting Vertebrate Animal-use Benchmarks for Agrochemical and GM Crop Testing to Facilitate Future Animal Reduction Efforts	0
Resources Round-up	0
A Novel Ex Vivo Blinking Method for Comparing the Corneal Residence Time of New Shampoo Formulations	0
Conference Diary	0
Spotlight on Three Rs Progress	0
Towards More Human and Humane Testing: The Role of the Device Supplier Industry	0
Resources Round-up	0
Spotlight on Three Rs Progress	0
Editorial	0
20 Years of Providing Custom Non-Animal-Derived Antibodies — A Review	0
2022 Michael Balls Award	0
Coculture of ARPE-19 Cells and Porcine Neural Retina as an Ex Vivo Retinal Model	0
Spotlight on Three Rs Progress	0
Editorial	0
Editorial	0
The Use of In Silico Methods to Identify and Assess Antigenic Regions Suitable for the Development of Peptide-based Pan-viral Vaccines	0
Is UK Animal Research Governance Facing a ‘New Normal’? Considering the Risks and Benefits of ‘Going Online’	0
Resources Round-up	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
Thanks to Reviewers	0
Editorial	0
Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells	0
Evaluation of a Low-cost Renal Simulator for the Diagnostic Ultrasound Training of Veterinary Medicine Students	0
2025 Michael Balls Award	0
Key Challenges and Recommendations for In Vitro Testing of Tobacco Products for Regulatory Applications: Consideration of Test Materials and Exposure Parameters	0
Editorial	0
It’s Time to Review the Three Rs, to Make them More Fit for Purpose in the 21st Century	0
Assessment of the Culture of Care in Sri Lankan laboratory animal facilities and identifying challenges: A laboratory animal care staff-centered approach	0
The Accomplishments of KoCVAM in the Development and Implementation of Alternative Methods in Korea	0
Biomedical Research on Substances of Abuse: The Italian Case Study	0
A Comparison of European Union and Turkish Reports on the Statistics of Experimental Animal Use in Toxicity Testing	0