Atla-Alternatives to Laboratory Animals

Article	Citations
Extension of the Virtual Cell Based Assay from a 2-D to a 3-D Cell Culture Model	102
Spotlight on Three Rs Progress	37
Spotlight on Three Rs Progress	36
The Use of Human Tissues for Research: What Investigators Need to Know	31
The Safety Assessment of Cosmetic Perfumes by Using In Chemico and In Vitro Methods in Combination with GC-MS/MS Analysis	23
Conference Diary	22
Combinatorial induction with valproic acid and fibroblast growth factor-9 enhances neural differentiation and cell fate commitment in adipose tissue-derived mesenchymal stem cells	16
Toxicological Profiling of Potential Shikimate Kinase Inhibitors Against Mycobacterium tuberculosis	14
The Development of an Animal Product-free, Precision-cut Lung Slice Cryopreservation and Post-thaw Culture Method	14
Progress and Remaining Opportunities to Increase the Use of Animal-free Antibodies in the USA	13
Resources Round-up	11
Dr Manfred Liebsch: Former Head of ZEBET and ATLA Editorial Board Member	10
Resources Round-up	10
Brazilian National Network of Alternative Methods (RENAMA) 10th Anniversary: Meeting of the Associated Laboratories, May 2022	9
A History of the Development of Alternatives to Animals in Research and Testing	9
Conference Diary	9
Development of Sensitive In Vitro Protocols for the Biocompatibility Testing of Medical Devices and Pharmaceuticals Intended for Contact with the Eyes: Acute Irritation and Phototoxicity Assess	9
Editorial	9
Report of the First ONTOX Stakeholder Network Meeting: Digging Under the Surface of ONTOX Together With the Stakeholders	9
In Silico Prediction of Human Clinical Pharmacokinetics with ANDROMEDA by Prosilico: Predictions for an Established Benchmarking Data Set, a Modern Small Drug Data Set, and a Comparison with La	8
The Rise of Three Rs Centres and Platforms in Europe*	7
Spotlight on Three Rs Progress	7
Editorial	7
2021 Michael Balls Award	6
The 19th FRAME Annual Lecture, November 2022: Safer Chemicals and Sustainable Innovation Will Be Achieved by Regulatory Use of Modern Safety Science, Not by More Animal Testing	6

Editorial	6
Editorial	6
A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of Bothrops jararaca Venom and Antivenom: An Intralaboratory Pre-validation Study	5
Reviewing Current Guidance for the ‘R’ of Replacement and Rethinking it with the ‘Replacement Checklist’	5
Editorial	5
Fenugreek Seed Extract Regulates Human Umbilical Vein Endothelial Cell Angiogenesis and Proliferation via the PI3K/Akt/Cyclin D1 Pathway	5
Thanks to Reviewers	5
Addressing Animal Welfare Issues in Fetal Blood Collection for Fetal Bovine Serum Production	5
The Numbers of Animals Used in Mexico for Scientific and Educational Purposes	4
Editorial	4
Spotlight on Three Rs Progress	4
Development of Two Korean IACUC Guidance Documents to Foster Implementation of the Three Rs	4
Editorial	4
Optimisation of a Method for the Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells Toward Renal Epithelial-like Cells	4
Corrigendum to Case Studies Exemplifying the Transition to Animal Component-Free Cell Culture	4
Book Review: Lab dog: A beagle and his human investigate the surprising world of animal research Melanie D.G. Kaplan (2025). Lab Dog: A Beagle and His Human Investigate the Surprising World of Animal	4
Resources Round-up	4
The Use of 3-D Models of Echocardiographic Imaging Planes for Teaching Echocardiography Techniques for Use in Dogs and Cats	3
Editorial	3
Accelerating Animal Replacement: How Universities Can Lead — Results of a One-Day Expert Workshop in Zurich, Switzerland	3
JRC Summer School 2021	3
Lessons for the Future of NAMs from History, Philosophy and Social Studies of Science	3
Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Second International Conference on 3Rs Research and Progress, Hyderabad, 2021	3
Case Studies Exemplifying the Transition to Animal Component-free Cell Culture	3
A New ATLA Associate Editor	3
Editorial	3
Determining the pathogenicity of uropathogenic Escherichia coli strains in the Galleria mellonella larvae model	3
Spotlight on Three Rs Progress	3
Editorial	3
Editorial	3
Conference Diary	3
The Application of New Approach Methodologies in Respiratory Disease Research: Their Role in Improving Translational Medicine from Bench to Bedside	3
Modelling the Sorafenib-resistant Liver Cancer Microenvironment by Using 3-D Spheroids	2
Spotlight on Three Rs Progress	2
Resources Round-up	2
Editorial	2
The Current Status and Work of Three Rs Centres and Platforms in Europe*	2
Resources Round-up	2
Transparency in Non-Technical Project Summaries to Promote the Three Rs in Respiratory Disease Research	2
ATLA Best Poster Award	2
Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)	2
Use of the Allium cepa Model to Assess the Cytogenotoxicity of Luffariella herdmani Marine Sponge Extract	2
An Evaluation of the Replacement of Animal-derived Biomaterials in Human Primary Cell Culture	2
Resources Round-up	1
A Comparison of Mesenchymal Stem Cell-derived Hepatocyte-like Cells and HepG2 Cells for Use in Drug-Induced Liver Injury Studies	1
Resources Round-up	1
Spotlight on Three Rs Progress	1
Barriers to the Use of Recombinant Bacterial Endotoxins Test Methods in Parenteral Drug, Vaccine and Device Safety Testing	1
The Evolution of Regulatory Toxicology: Where is the Gardener?	1
The Effects of Urolithin B and Auraptene on Quinolinic Acid-induced Toxicity in the SH-SY5Y Neuroblastoma Cell Line	1

Academic Training to Promote the Implementation of Alternative Methods and the Three Rs in the Field of Toxicology	1
Implementing the EURL ECVAM Recommendation on Non-Animal-Derived Antibodies in One EU Member State — Denmark	1
Editorial	1
Biobank Personnel — The Key to its Success	1
Journeying Through Journals: The Publishing Process and How to Maximise Research Impact	1
Conference Diary	1
Conference Diary	1
Poster Prizes 2021	1
JRC Summer School on Non-animal Approaches in Science, May 2021	1
Spotlight on Three Rs Progress	1
Resources Round-up	1
Assessment of a Low-cost Canine Uterine Simulator as a Tool for Teaching the Ovariohysterectomy Technique to Veterinary Students	1
Resources Round-up	1
Conference Diary	1
Conference Diary	1
Conference Diary	1
The Use of Simulators for Teaching Practical Clinical Skills to Veterinary Students — A Review	1
Spotlight on Three Rs Progress	1
Alternatives to Laboratory Animals: Trends in Replacement and the Three Rs	1
The VEGAHUB Platform: The Philosophy and the Tools	1
Announcing the new ATLA Associate Editors	1
Editorial	1
Conference Diary	1
Corrigendum to Report of the First ONTOX Hackathon: Hack to Save Lives and Avoid Animal Suffering. The Use of Artificial Intelligence in Toxicology — A Potential Driver for Reducing/Replacing Laborato	1
An Accessible Nephrectomy and Cystotomy Simulator for Use in Teaching Small Animal Surgery	1
Resources Round-up	1
Conference Diary	1
A Low-fidelity Simulator for the Development of Vascular Ligation Skills	0
Message from FRAME	0
Editorial	0
Spotlight on Three Rs Progress	0
Acute Toxicity Assays with the Artemia salina Model: Assessment of Variables	0
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an In Vitro Model Based on Differentiated PC12 Cells	0
Spotlight on Three Rs Progress	0
Conference Diary	0
The Istanbul Workshop organised by the ‘IMPROVE’ COST Action Ethics Crossover Group	0
It’s Time to Review the Three Rs, to Make them More Fit for Purpose in the 21st Century	0
Spotlight on Three Rs Progress	0
Conference Diary	0
2025 Michael Balls Award	0
The Use of an In Chemico Digestibility Assay to Reduce the In Vivo Fish Bioaccumulation Testing of Nanomaterials	0
Conference Diary	0
Is UK Animal Research Governance Facing a ‘New Normal’? Considering the Risks and Benefits of ‘Going Online’	0
Editorial	0
Assessment of the Culture of Care in Sri Lankan laboratory animal facilities and identifying challenges: A laboratory animal care staff-centered approach	0
The Perceptions of Students and Lecturers on the Use of Animals in Biomedical Science Undergraduate Education in Brazil	0
Expression of Concern: “Fenugreek Seed Extract Regulates Human Umbilical Vein Endothelial Cell Angiogenesis and Proliferation via the PI3K/Akt/Cyclin D1 Pathway”	0
Conference Diary	0
Conference Diary	0
Mesoporous bioactive glass nanoparticles exhibit intrinsic angiogenic potential in the chorioallantoic membrane assay, without the addition of exogenous cells	0
Rethinking the Three Rs: Growing Momentum Toward a New Gold Standard in Australian Research	0
Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells	0
Chitosan–Calcium Aluminate as a Cell-homing Scaffold: Its Bioactivity Testing in a Microphysiological Dental Pulp Platform	0
Resources Round-up	0
Adapting to Changes in Publishing When Searching for Alternatives and Reporting on Animal Research: A Librarian’s Perspective	0
In Silico Prediction of Eye Irritation Using Hansen Solubility Parameters and Predicted pKa Values	0
Editorial	0
Chemicals 2.0 and Why We Need to Bypass the Gold Standard in Regulatory Toxicology	0
Development of an Accessible Gene Expression Bioinformatics Pipeline to Study Driver Mutations of Colorectal Cancer	0
Optimisation of Conditions for the Formation of Spheroids of Head and Neck Squamous Cell Carcinoma Cell Lines for Use as Animal Alternatives	0
A Novel Ex Vivo Blinking Method for Comparing the Corneal Residence Time of New Shampoo Formulations	0
Educators’ Views on the Use of Dissection and Dissection Alternatives in American Biology Classrooms	0
Resources Round-up	0
EURL ECVAM Activities	0
A Short History of the Consideration of Sex Differences in Biomedical Research — Lessons for the In Vitro Community from Animal Models and Human Clinical Trials	0
Conference Diary	0
Biosensor-based approaches: Exploring hapten – protein interactions for the prediction of skin sensitisation	0
Coculture of ARPE-19 Cells and Porcine Neural Retina as an Ex Vivo Retinal Model	0
The Capability Maturity Model as a Measure of Culture of Care in Laboratory Animal Science	0
Conference Diary	0
How to Foster ‘New Approach Methodology’ Toxicologists	0
In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos, Munronia pinnata and Psidium guajava	0
Determination of the Microinjected Cytosolic Concentration by Using Mathematical Models of Fluid Dynamics	0
Book Review: Zebrafish Model for Biomedical Research	0
Resources Round-up	0
Spotlight on Three Rs Progress	0
The 3Ranker: An AI-based Algorithm for Finding Non-animal Alternative Methods	0
Editorial	0
An Approach to Setting Vertebrate Animal-use Benchmarks for Agrochemical and GM Crop Testing to Facilitate Future Animal Reduction Efforts	0
Cardiac Transcription Regulators Differentiate Human Umbilical Cord Mesenchymal Stem Cells into Cardiac Cells	0

The Prevalence of Reported Gavage Incidents in Repeated Dose Toxicity Studies Involving Rats Conducted Between 2004–2023	0
Resources Round-up	0
An Evaluation of Different Types of Peptone as Partial Substitutes for Animal-derived Serum in Vero Cell Culture	0
Development of a 3D-printed canine cardiac disease model for interventional procedure training	0
A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study	0
20 Years of Providing Custom Non-Animal-Derived Antibodies — A Review	0
2022 Michael Balls Award	0
Spotlight on Three Rs Progress	0
Conference Diary	0
Resources Round-up	0
Resources Round-up	0
The Use of In Silico Methods to Identify and Assess Antigenic Regions Suitable for the Development of Peptide-based Pan-viral Vaccines	0
Conference Diary	0
Dr Richard Clothier: FRAME Trustee, Founding Director of the FRAME Laboratory and ATLA Editorial Board Member	0
Cigarette Smoke Constituents and Nicotine Differentially Affect Cytokine Production by Human Macrophages Stimulated by TLR Ligands In Vitro: Considerations for a Standardised Protocol	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
Xeno-free alternatives to the use of fetal bovine serum in head and neck cancer explant culture	0
Conference Diary	0
Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022	0
Report of the First ONTOX Hackathon: Hack to Save Lives and Avoid Animal Suffering. The Use of Artificial Intelligence in Toxicology — A Potential Driver for Reducing/Replacing Laboratory Animals in t	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Editorial	0
Recent ATLA Awards	0
Spotlight on Three Rs Progress	0
Biomedical Research on Substances of Abuse: The Italian Case Study	0
Spotlight on Three Rs Progress	0
2023 Michael Balls Award	0
Editorial	0
Poor Translatability of Biomedical Research Using Animals — A Narrative Review	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Editorial	0
Thanks to Reviewers	0
Editorial	0
Resources Round-up	0
Resources Round-up	0
Ciliary Beat Frequency: Proceedings and Recommendations from a Multi-laboratory Ring Trial Using 3-D Reconstituted Human Airway Epithelium to Model Mucociliary Clearance	0
Resources Round-up	0
Thanks to Reviewers	0
The Recombinant Antibodies & Mimetics Database: Redefining the future of antibody use in science	0
Permutation Tests Are a Useful Alternative Approach for Statistical Hypothesis Testing in Small Sample Sizes	0
A History of Regulatory Animal Testing: What Can We Learn?	0
Charting a new path, replacing animals in biomedical research	0
Conference Diary	0
The Use of MPS in Three Rs and Regulatory Applications: Perspectives From Developers on Stakeholder Responsibilities	0
Resources Round-up	0
A History of the Development of Alternatives to Animals in Research and Testing	0
Calling all students and early-career researchers — Join us for the second Summer School on Innovative Approaches in Science!	0
Spotlight on Three Rs Progress	0
Thanks to Reviewers	0
A Comparison of European Union and Turkish Reports on the Statistics of Experimental Animal Use in Toxicity Testing	0
Editorial	0
Editorial	0
In Silico Phototoxicity Prediction of Drugs and Chemicals by using Derek Nexus and QSAR Toolbox	0
Key Challenges and Recommendations for In Vitro Testing of Tobacco Products for Regulatory Applications: Consideration of Test Materials and Exposure Parameters	0
Editorial	0
Towards More Human and Humane Testing: The Role of the Device Supplier Industry	0
Resources Round-up	0
Evaluation of a Low-cost Renal Simulator for the Diagnostic Ultrasound Training of Veterinary Medicine Students	0
Resources Round-up	0
Key Challenges for In Vitro Testing of Tobacco Products for Regulatory Applications: Recommendations for the In Vitro Mouse Lymphoma Assay	0
Spotlight on Three Rs Progress	0
Conference Diary	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
Breaking down the barriers to animal-free research	0
Celebrating Professor Horst Spielmann’s 80th Birthday	0
Evaluating the Impact of Hair Care Product Exposure on Hair Follicle and Scalp Health	0
The Accomplishments of KoCVAM in the Development and Implementation of Alternative Methods in Korea	0
Conference Diary	0
Conference Diary	0
Spotlight on Three Rs Progress	0