Atla-Alternatives to Laboratory Animals

Article	Citations
The Safety Assessment of Cosmetic Perfumes by Using In Chemico and In Vitro Methods in Combination with GC	166
Spotlight on Three Rs Progress	38
Spotlight on Three Rs Progress	26
Evaluation of the antinociceptive effects of gabapentin using Gromphadorhina portentosa as a non-vertebrate in vivo	19
The Use of Human Tissues for Research: What Investigators Need to Know	19
The Development of an Animal Product-free, Precision-cut Lung Slice Cryopreservation and Post-thaw Culture Method	18
Combinatorial induction with valproic acid and fibroblast growth factor-9 enhances neural differentiation and cell fate commitment in adipose tissue-derived mesenchymal stem cells	16
Conference Diary	16
Toxicological Profiling of Potential Shikimate Kinase Inhibitors Against Mycobacterium tuberculosis	13
Conference Diary	12
Development of Sensitive In Vitro Protocols for the Biocompatibility Testing of Medical Devices and Pharmaceuticals Intended for Contact with the Eyes: A	11
Conference Diary	11
Brazilian National Network of Alternative Methods (RENAMA) 10th Anniversary: Meeting of the Associated Laboratories, May 2022	11
Progress and Remaining Opportunities to Increase the Use of Animal-free Antibodies in the USA	11
A History of the Development of Alternatives to Animals in Research and Testing	10
Resources Round-up	10
In Silico Prediction of Human Clinical Pharmacokinetics with ANDROMEDA by Prosilico: Predictions for an Established Benchmarking Data Set, a Modern Small Drug Data Set, and	9
Resources Round-up	9
Report of the First ONTOX Stakeholder Network Meeting: Digging Under the Surface of ONTOX Together With the Stakeholders	9
Dr Manfred Liebsch: Former Head of ZEBET and ATLA Editorial Board Member	9
Editorial	9
Editorial	8
2021 Michael Balls Award	7
Editorial	7
Addressing Animal Welfare Issues in Fetal Blood Collection for Fetal Bovine Serum Production	7

Reviewing Current Guidance for the ‘R’ of Replacement and Rethinking it with the ‘Replacement Checklist’	7
Editorial	7
The 19th FRAME Annual Lecture, November 2022: Safer Chemicals and Sustainable Innovation Will Be Achieved by Regulatory Use of Modern Safety Science, Not by More Animal Testing	7
Resources Round-up	6
Spotlight on Three Rs Progress	6
The impossible made possible through the use of New Approach Methodologies: Dermal safety assessment of potential insect repellent or insecticidal active ingredients using a high-throughput approach t	6
Spotlight on Three Rs progress	6
Fenugreek Seed Extract Regulates Human Umbilical Vein Endothelial Cell Angiogenesis and Proliferation via the PI3K/Akt/Cyclin D1 Pathway	6
Thanks to Reviewers	5
Development of Two Korean IACUC Guidance Documents to Foster Implementation of the Three Rs	5
A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of Bothrops jararaca Venom and Antivenom: An Intralaboratory Pre-valid	5
Synthetic substrates as alternatives to bovine enamel in restorative dental materials research — A review	5
Editorial	5
The Numbers of Animals Used in Mexico for Scientific and Educational Purposes	5
Spotlight on Three Rs Progress	5
Editorial	4
Optimisation of a Method for the Differentiation of Human Umbilical Cord-derived Mesenchymal Stem Cells Toward Renal Epithelial-like Cells	4
Accelerating Animal Replacement: How Universities Can Lead — Results of a One-Day Expert Workshop in Zurich, Switzerland	4
Case Studies Exemplifying the Transition to Animal Component-free Cell Culture	4
Resources Round-up	4
Editorial	4
Human NT2/D1-derived early neural progenitor cells immobilised in alginate microfibres as an in vitro 3D system for neurotoxicity testing	4
Book Review: Lab dog: A beagle and his human investigate the surprising world of animal research Melanie D.G. Kaplan (2025). Lab Dog: A Beagle and His Human Investigate the Surprising World of Animal	4
Corrigendum to Case Studies Exemplifying the Transition to Animal Component-Free Cell Culture	4
Editorial	4
Spotlight on Three Rs Progress	3
Replacing immunisation-dependent antibody reagent production: Challenges under the UK Replacing Animals in Science	3
Introducing the COST Action ‘Improving the Quality of Biomedical Science with 3Rs Concepts’ (IMPROVE)	3
Editorial	3
Lessons for the Future of NAMs from History, Philosophy and Social Studies of Science	3
The Application of New Approach Methodologies in Respiratory Disease Research: Their Role in Improving Translational Medicine from Bench to Bedside	3
The Current Status and Work of Three Rs Centres and Platforms in Europe*	3
Editorial	3
Determining the pathogenicity of uropathogenic Escherichia coli strains in the Galleria mellonella larvae	3
Resources Round-up	2
Spotlight on Three Rs Progress	2
Use of the Allium cepa Model to Assess the Cytogenotoxicity of Luffariella herdmani Marine Sponge Extract	2
Conference Diary	2
Transparency in Non-Technical Project Summaries to Promote the Three Rs in Respiratory Disease Research	2
Modelling the Sorafenib-resistant Liver Cancer Microenvironment by Using 3-D Spheroids	2
Conference Diary	1
Editorial	1
Spotlight on Three Rs Progress	1
Conference Diary	1
Conference Diary	1
Conference Diary	1
Conference Diary	1
The Effects of Urolithin B and Auraptene on Quinolinic Acid-induced Toxicity in the SH-SY5Y Neuroblastoma Cell Line	1
Editorial	1
An Accessible Nephrectomy and Cystotomy Simulator for Use in Teaching Small Animal Surgery	1

Announcing the new ATLA Associate Editors	1
A low-cost balloon-based simulator for teaching surgical knot techniques to veterinary students	1
Resources Round-up	1
Resources Round-up	1
Evaluating the translational value of preclinical models: Available tools and frameworks, challenges and strategies	1
Assessment of a Low-cost Canine Uterine Simulator as a Tool for Teaching the Ovariohysterectomy Technique to Veterinary Students	1
Editorial	1
An Evaluation of the Replacement of Animal-derived Biomaterials in Human Primary Cell Culture	1
Journeying Through Journals: The Publishing Process and How to Maximise Research Impact	1
Emerging bioethical conflicts: One Health and animal experimentation	1
Resources Round-up	1
Barriers to the Use of Recombinant Bacterial Endotoxins Test Methods in Parenteral Drug, Vaccine and Device Safety Testing	1
Conference Diary	1
Conference Diary	1
Biobank Personnel — The Key to its Success	1
Corrigendum to Report of the First ONTOX Hackathon: Hack to Save Lives and Avoid Animal Suffering. The Use of Artificial Intelligence in Toxicology — A Potential Driver for Reducing/Replacing Laborato	1
The Evolution of Regulatory Toxicology: Where is the Gardener?	1
Resources Round-up	1
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Chemicals 2.0 and Why We Need to Bypass the Gold Standard in Regulatory Toxicology	0
Chitosan–Calcium Aluminate as a Cell-homing Scaffold: Its Bioactivity Testing in a Microphysiological Dental Pulp Platform	0
Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells	0
Conference Diary	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Spotlight on Three Rs Progress	0
Editorial	0
Time flies: A bibliometric exploration of evolving trends in Drosophila -based research	0
Implementing the EURL ECVAM Recommendation on Non-Animal-Derived Antibodies in One EU Member State — Denmark	0
A History of Regulatory Animal Testing: What Can We Learn?	0
Spotlight on Three Rs Progress	0
Thanks to Reviewers	0
An Evaluation of Different Types of Peptone as Partial Substitutes for Animal-derived Serum in Vero Cell Culture	0
Book Review: Zebrafish Model for Biomedical Research	0
Spotlight on Three Rs Progress	0
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an In Vitro Model Based on Differentiated PC12 Cells	0
Resources Round-up	0
Resources Round-up	0
Conference Diary	0
Xeno-free alternatives to the use of fetal bovine serum in head and neck cancer explant culture	0
2025 Michael Balls Award	0
Conference Diary	0
The 3Ranker: An AI-based Algorithm for Finding Non-animal Alternative Methods	0
2026 Michael Balls Award	0
Charting a new path, replacing animals in biomedical research	0
Conference Diary	0
Redefining the gold standard and recommendations to accelerate the adoption of New Approach Methodologies (NAMs) in support of the Three Rs	0
Conference Diary	0
Editorial	0
Spotlight on Three Rs Progress	0
Conference Diary	0
20 Years of Providing Custom Non-Animal-Derived Antibodies — A Review	0
Conference Diary	0
Conference Diary	0
Spotlight on Three Rs Progress	0
Editorial	0
Development of an Accessible Gene Expression Bioinformatics Pipeline to Study Driver Mutations of Colorectal Cancer	0
Conference Diary	0
Key Challenges and Recommendations for In Vitro Testing of Tobacco Products for Regulatory Applications: Consideration of Test Materials and Exposure Par	0
Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022	0
A Novel Ex Vivo Blinking Method for Comparing the Corneal Residence Time of New Shampoo Formulations	0
Report of the First ONTOX Hackathon: Hack to Save Lives and Avoid Animal Suffering. The Use of Artificial Intelligence in Toxicology — A Potential Driver for Reducing/Replacing Laboratory Animals in t	0
Editorial	0
Editorial	0
Biosensor-based approaches: Exploring hapten – protein interactions for the prediction of skin sensitisation	0
The Use of In Silico Methods to Identify and Assess Antigenic Regions Suitable for the Development of Peptide-based Pan-viral Vaccines	0
Biomedical Research on Substances of Abuse: The Italian Case Study	0
Editorial	0
Poor Translatability of Biomedical Research Using Animals — A Narrative Review	0
Conference Diary	0
Editorial	0
Resources Round-up	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
Development of a 3D-printed canine cardiac disease model for interventional procedure training	0
In Vitro Dengue Virus Inhibition by Aqueous Extracts of Aegle marmelos , Munronia pinnata	0
Cardiac Transcription Regulators Differentiate Human Umbilical Cord Mesenchymal Stem Cells into Cardiac Cells	0
The Recombinant Antibodies & Mimetics Database: Redefining the future of antibody use in science	0

It’s Time to Review the Three Rs, to Make them More Fit for Purpose in the 21st Century	0
Editorial	0
In Silico Prediction of Eye Irritation Using Hansen Solubility Parameters and Predicted pKa Values	0
An Approach to Setting Vertebrate Animal-use Benchmarks for Agrochemical and GM Crop Testing to Facilitate Future Animal Reduction Efforts	0
Conference Diary	0
A Human Cell-based Assay to Assess the Induction of Vasculature Formation for Non-genotoxic Carcinogenicity Testing Purposes: A Pilot Study	0
The Capability Maturity Model as a Measure of Culture of Care in Laboratory Animal Science	0
Resources Round-up	0
The Use of MPS in Three Rs and Regulatory Applications: Perspectives From Developers on Stakeholder Responsibilities	0
Cigarette Smoke Constituents and Nicotine Differentially Affect Cytokine Production by Human Macrophages Stimulated by TLR Ligands In Vitro : Considerati	0
In Silico Phototoxicity Prediction of Drugs and Chemicals by using Derek Nexus and QSAR Toolbox	0
Dr Richard Clothier: FRAME Trustee, Founding Director of the FRAME Laboratory and ATLA Editorial Board Member	0
Thanks to Reviewers	0
Mesoporous bioactive glass nanoparticles exhibit intrinsic angiogenic potential in the chorioallantoic membrane assay, without the addition of exogenous cells	0
Resources Round-up	0
A Comparison of European Union and Turkish Reports on the Statistics of Experimental Animal Use in Toxicity Testing	0
Editorial	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
A systematic review of the role of laboratory animal veterinarians in promoting One Welfare	0
Is UK Animal Research Governance Facing a ‘New Normal’? Considering the Risks and Benefits of ‘Going Online’	0
The use of skin explants in Brazil: A scoping review of applications, methodologies and research trends	0
A Short History of the Consideration of Sex Differences in Biomedical Research — Lessons for the In Vitro Community from Animal Models and Human Clinical	0
Evaluating the Impact of Hair Care Product Exposure on Hair Follicle and Scalp Health	0
Recent ATLA Awards	0
The Accomplishments of KoCVAM in the Development and Implementation of Alternative Methods in Korea	0
Optimisation of Conditions for the Formation of Spheroids of Head and Neck Squamous Cell Carcinoma Cell Lines for Use as Animal Alternatives	0
Breaking down the barriers to animal-free research	0
Editorial	0
Rethinking the Three Rs: Growing Momentum Toward a New Gold Standard in Australian Research	0
Resources Round-up	0
Resources Round-up	0
Ciliary Beat Frequency: Proceedings and Recommendations from a Multi-laboratory Ring Trial Using 3-D Reconstituted Human Airway Epithelium to Model Mucociliary Clearance	0
Resources Round-up	0
Acute Toxicity Assays with the Artemia salina Model: Assessment of Variables	0
Conference Diary	0
Editorial	0
Resources Round-up	0
Spotlight on Three Rs Progress	0
Resources Round-up	0
Legal incoherence in EU chemical safety law: A proportionality-based analysis of REACH and CLP	0
Key Challenges for In Vitro Testing of Tobacco Products for Regulatory Applications: Recommendations for the In Vitro	0
Spotlight on Three Rs Progress	0
The Istanbul Workshop organised by the ‘IMPROVE’ COST Action Ethics Crossover Group	0
A History of the Development of Alternatives to Animals in Research and Testing	0
Permutation Tests Are a Useful Alternative Approach for Statistical Hypothesis Testing in Small Sample Sizes	0
Resources Round-up	0
Spotlight on Three Rs Progress	0
Expression of Concern: “Fenugreek Seed Extract Regulates Human Umbilical Vein Endothelial Cell Angiogenesis and Proliferation via the PI3K/Akt/Cyclin D1 Pathway”	0
Message from FRAME	0
Spotlight on Three Rs Progress	0
The Prevalence of Reported Gavage Incidents in Repeated Dose Toxicity Studies Involving Rats Conducted Between 2004–2023	0
Conference Diary	0
Thanks to reviewers	0
2023 Michael Balls Award	0
The Use of an In Chemico Digestibility Assay to Reduce the In Vivo Fish Bioaccumulation Testing of Nanomat	0
2022 Michael Balls Award	0
Conference Diary	0
Assessment of the Culture of Care in Sri Lankan laboratory animal facilities and identifying challenges: A laboratory animal care staff-centered approach	0
Resources Round-up	0
Resources Round-up	0