Journal of Biotechnology

Article	Citations
Rational engineering of phospholipase C from Bacillus cereus HSL3 for simultaneous thermostability and activity improvement	189
Branched-chain polyamine beads for sensitive recovery and detection of low-copy DNA in aqueous solutions	170
Enhancement of the production of terpenoid and flavonoid secondary metabolites in the ground and aerial parts of licorice composite plant in a hydroponic system	126
In silico design of CMV promoter binding oligonucleotides and their impact on inhibition of gene expression in Chinese hamster ovary cells	119
In vitro synthesis of oryzamutaic acid H using recombinant L-lysine ε-dehydrogenase from Agrobacterium tumefaciens	74
Using gel microdroplets to develop a simple high-throughput screening platform for oleaginous microorganisms	73
Fungal elicitors increase cell biomass, pyrroloquinazoline alkaloids production and gene expression levels of biosynthetic pathways in Adhatoda vasica Nees cell cultures	62
Epitope mapping of nanobodies binding the Alzheimer’s disease receptor SORLA	56
Retraction notice to “A recombinant H1 histone based system for efficient delivery of nucleic acids” [J. Biotechnol. 105 (2003) 215–226]	55
Engineering the activity and thermostability of a carboxylic acid reductase in the conversion of vanillic acid to vanillin	52
Marine-derived Streptomyces sp. isolated from the Persian Gulf as a novel source of melanin	51
Investigating subpopulation dynamics in clonal CHO-K1 cells with single-cell RNA sequencing	49
Ethanol alleviates glucose stress from Euglena gracilis	48
Development of a serum-free producer cell line generation process for scalable and efficient rAAV production for gene therapy applications	45
Development of transgenic algae strain expressing CTB-M2e fusion gene an approach towards the development of a universal edible vaccine in algae	44
The physiological role of gamma-aminobutyric acid in relieving the effect of furfural inhibitor for improvement the production of lipid in D. intermedius Z8	41
The complex patent situation regarding antibody Fc engineering	38
Optimisation of coumaric acid production from aromatic amino acids in Kluyveromyces marxianus	37
Short-term effect of biologically treated sugarcane vinasse on the growth of Triticum aestivum L. (wheat) and soil properties	36
In vitro response of sugarcane (Saccharum spp. Hybrid) plantlets to flooding stress	36
A novel dynamic flux balance analysis for modeling CHO cell fed-batch cultures with pH and temperature shifts	35
Methanol-induced metabolic disturbances in protein expression in Pichia pastoris: A review of the interaction between amino acid synthesis and higher alcohol pathways	35
Directional lactic acid production could mitigate antibiotic and ARGs pollution	34
Enhanced tolerance of Clostridium tyrobutyricum to lignin-derived phenolic acids by overexpressing native reductases	33
Engineering of a bacterial outer membrane vesicle to a nano-scale reactor for the biodegradation of β-lactam antibiotics	32

Editorial Board	32
OptMSP: A toolbox for designing optimal multi-stage (bio)processes	32
Magnetic carboxymethyl cellulose-silk fibroin hydrogel: A ternary nanobiocomposite exhibiting excellent biological activity and in vitro hyperthermia of cancer therapy	31
Regulation of hydraulic retention time on caproic acid production via two-phase anaerobic fermentation of Chinese cabbage waste with autopoietic electron donors	30
Enhanced methanol-xylose co-utilization strategy in Komagataella phaffii	29
Novel approaches to engineering autolysis: A scalable alternative for recovery of bioproducts.	28
Microwave-assisted biogenic synthesis of pure copper oxide nanoparticles by Lepidium sativum L. leaves extract: Characterization and evaluation of antibacterial activity	27
UV and chemically induced Halomonas smyrnensis mutants for enhanced levan productivity	26
Employment of light-inducible promoter in genetically engineered cyanobacteria for photosynthetic isobutanol production with simulated diurnal sunlight and CO2	26
Bioprocessing of pineapple leaf waste biomass using an integrated ultrasound-deep eutectic solvent pretreatment approach for improved bioethanol production	26
Plant-based synthesis, characterization approaches, applications and toxicity of silver nanoparticles: A comprehensive review	25
Enhancing heterotrophic lutein production in Chlorella protothecoides through combined phytohormone and nitrogen strategies	25
High-yield soluble production of recombinant β-keratin from Gallus gallus feathers using an experimental design approach	25
Enhancement of glutaminase production through protein design and host strain modification in Bacillus amyloliquefaciens	25
High-level expression and characterization of a highly active hyaluronate lyase HylC with significant potential in hyaluronan oligosaccharide preparation	24
Editorial Board	24
Advancements in transient overexpression systems for enhanced recombinant protein production in lettuce	23
A transposon system for random insertion of a gene expression cassette into the chromosome of Bacillus subtilis	23
Reviewing critical TRPM2 variants through a structure-function lens	23
Fragmentation of recombinant human interleukin-12 by matriptase in CHO cell culture	23
Efficient biosynthesis of ectoine in engineered Vibrio natriegens	22
Advancements in enzymatic biotransformation and bioactivities of rare ginsenosides: A review	22
Probing the interaction of zinc oxide nanorods with human serum albumin: A spectroscopic approach	22
Development of Stephania tetrandra S. MOORE hairy root culture process for tetrandrine production	22
Channel matters: Overcoming diffusion bottlenecks via loop engineering of LinD for enhanced isoprene production	22
Nano-managing silver and zinc as bio-conservational approach against pathogens of the honey bee	21
Establishment of high-efficiency hairy root and genetic transformation system in Cynanchum stauntonii	21
Enhancing the extraction of C-phycocyanin and water-soluble metabolites from Arthrospira platensis using pulsed electric field technology	21
In-line monitoring of bioreactor by Raman spectroscopy: Direct use of a standard-based model through cell-scattering correction	20
Elucidation of the fucose metabolism of probiotic Lactobacillus rhamnosus GG by metabolomic and flux balance analyses	20
Editorial Board	20
Saline extraction assisted by ultrasound: a method to obtain purified phycocyanin	20
Multiple genome analytics framework: The case of all SARS-CoV-2 complete variants	20
Ni2+-induced selective precipitation of His-tagged recombinant proteins shortens purification time while maintaining high yield	20
Development of a highly efficient microbial fermentation process of recombinant Escherichia coli for GABA production from glucose	20
Metabolic rewiring revealed by cell-specific rate analyses from nontargeted exometabolomics during simultaneous consumption of glucose and lactic acid in a CHO fed-batch process	20
Using synthetic biology to improve photosynthesis for sustainable food production	19
Ternary deep eutectic solvent (T-CuCl2-EG) for lignin removal from corn straw	19
Metabolic and biotechnological insights on the analysis of the Pdh bypass and acetate production in the yeast Dekkera bruxellensis	19
Efficiency and scalability in harvesting mammalian cell cultures: A scale-down approach to continuous centrifugation	19
Metabolic engineering of Schizosaccharomyces pombe for itaconic acid production	19
Improving thermostability of α-L-fucosidase from Pedobacter sp. via consensus-guided engineering and directed evolution	19
Thermal degradation kinetics and purification of C-phycocyanin from thermophilic and mesophilic cyanobacteria	19
Comparative analysis of manufacturability and binding of various bispecific antibody architectures	18
Bioconversion for ursodeoxycholic acid using β-cyclodextrin included-chenodeoxycholic acid via immobilized cells of Xanthomonas maltophilia	18
Development of thermostable dextranase from Streptococcus mutans (SmdexTM) through in silico design employing B-factor and Cartesian-ΔΔG	18
Production of recombinant human type I collagen homotrimers in CHO cells and their physicochemical and functional properties	18
Engineering AraC L9K for inducer-independent pBAD activation in Salmonella Gallinarum	18
High-throughput flow cytometry-based titration of a live attenuated rotavirus vaccine	18
Surface property effects in the immobilization of L-lactate oxidase on gold electrodes by self-assembled monolayers	18

Synthetic biology-driven innovations in triple-negative breast cancer: Integrating engineering design with targeted therapeutics	17
Microbial production of mevalonate	17
Low-cost alternative for the bioproduction of bioactive phenolic compounds of callus cultures from Cereus hildmannianus (K.) Schum	17
Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing	17
Alleviation of cadmium toxicity and minimizing its accumulation in rice plants by methyl jasmonate: Performance and mechanisms	17
Enhanced laccase separation from fermentation medium using cryogel columns	17
Weak promoters to drive selection marker expression: Improvement of cell line development process for therapeutic protein production in CHO-K1 cells	17
Leveraging mixed-mode filter during cell culture harvest to prevent antibody reduction	17
Enhanced activation of signaling pathway by recombinant human adiponectin from genome-edited chickens	16
A comprehensive overview of nanotechnology in sustainable agriculture	16
Genetic transformation of ‘Hamlin’ and ‘Valencia’ sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Livi	16
pH-dependent electrostatic interactions between enzymes and nanoparticles in Pickering emulsions – Influence on activity and droplet size	16
Editorial Board	16
Expediting adenovirus titer assays via an algorithmic live-cell imaging technique	16
Tailored base editing toolkits for functional genomics and metabolic engineering in the halophile Salinivibrio	16
Microbial production of single cell oil from biowaste-derived volatile fatty acids using a newly identified oleaginous Cyberlindnera jadinii	15
Direct biosynthesis of medium-chain fatty acid methyl esters in a plant host using a rationally designed SABATH methyltransferase	15
Novel efficient enzymatic synthesis of the key-reaction intermediate of PET depolymerization, mono(2-hydroxyethyl terephthalate) – MHET	15
Extension of the circulatory half-life of recombinant ecallantide via albumin fusion without loss of anti-kallikrein activity	15
Light intensity and spectral quality modulation for improved growth kinetics and biochemical composition of Chlamydomonas reinhardtii	15
Ethanolysis of degummed soybean oil using magnetic CLEAs from Eversa® Transform	15
Hemp tea waste-immobilized lipase for the synthesis of alkyl oleates in solvent free systems	15
Cd2+ tolerance and removal mechanisms of Serratia marcescens KMR-3	15
Rational comparison of biohydrogen production using Clostridium species through dark fermentation during anaerobic batch processes	15
Ozonation process causes changes in PARP-1 expression and the metabolism of NADPH in strawberry fruit during storage	15
Development of a new extraction method and functional analysis of phycocyanobilin from unique filamentous cyanobacteria	15
QTL mapping in sesame (Sesamum indicum L.): A review	14
A novel directed evolution approach for co-evolution of β-glucosidase activity and organic acid tolerance	14
Engineered praseodymium sulfide nanocarrier and supramolecular association of anticancer drug for effective delivery to breast cancer cells	14
Effect of selenium and tungsten on cell growth and metabolite production in syngas fermentation using “Clostridium autoethanogenum”	14
Halophilic filamentous fungi and their enzymes: Potential biotechnological applications	14
Integrating genetic assortment and molecular insights for climate-resilient breeding to unravel drought tolerance in cotton	14
Dynamic regulation of ROS stress in the accumulation of L-lysine in Escherichia coli	14
Enhanced anthraquinone biosynthesis in Damnacanthus major cells via medium optimization and fed-batch bioreactor culture	14
Editorial Board	14
Enhancing secondary metabolite biosynthesis from ethnomedicinal plant Solanum nigrum L. through cytogenetically stable mass propagation, transgenic hairy root induction, and using different LED light	14
UV-ARTP compound mutagenesis breeding improves macrolactins production of Bacillus siamensis and reveals metabolism changes by proteomic	14
Next level of p-phenylene diisothiocyanate-based covalent immobilization of β-D-galactosidase: Technical optimization as an application	14
Persistent low PHB accumulation during pilot-scale gas fermentation in Cupriavidus necator	13
Genomic features of recombinant CHO clones arising from transposon-based and randomized integration	13
Editorial Board	13
Impact of humanization scaffold design on the functional activity of FGFR2-targeting chicken scFvs	13
Cell specific nutrient feeding in perfusion mode enhances hMSC growth in stirred tank bioreactor process	13
Hairy roots as a biotechnological tool for medicinal plant secondary metabolites: A systematic review	13
Corrigendum to “A cheap two-step cultivation of Phaeodactylum tricornutum for increased TAG production and differential expression of TAG biosynthesis associated genes” [J. Biotechnol. 354 (2022) 53–6	13
Pretreatment of lignocellulosic biogas substrates by filamentous fungi	13
Editorial Board	13
Construction of an E. coli cell factory for ergothioneine through SAM-cycle enhancement and pathway reconstruction	13
Thermodynamics of microbial consortia: Enthalpies and Gibbs energies of microorganism live matter and macromolecules of E. coli, G. oxydans, P. fluorescens, S. thermophilus and P. chrysogenum	13
The CRISPR Cas patent files, part 4: All back to zero, think again!	13
P3HT-based organic field effect transistor for low-cost, label-free detection of immunoglobulin G	12
Priming with EDTA, IAA and Fe alleviates Pb toxicity in Trigonella Foneum graecum L. growth: Phytochemicals and secondary metabolites	12
High temperature drives torularhodin-dominant carotenoid overaccumulation in Rhodosporidiobolus odoratus XQR via coordinated transcriptomic–metabolomic reprogramming	12
Editorial Board	12
Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica	12
Progress on production of malic acid and succinic acid by industrially-important engineered microorganisms	12
Lipase-mediated alcoholysis for in situ production of ester bioaromas in licuri oil for cosmetic applications	12
Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design	12
Application of a novel virus-like particle platform to the development of Neisseria vaccines	12
Expression of a recombinant protein by an acetic acid bacterial host	12
Investigation into optimizing fermentation processes to enhance uric acid degradation by probiotics	11
Engineering Saccharomyces boulardii for cell surface display of heterologous protein	11
Harnessing nature's catalysts: Advances in enzymatic alkene cleavage	11
High specificity of MsmCAR toward 4-hydroxyvaleric acid enables efficient 1,4-pentanediol production from biomass-derived levulinic acid	11
Customized design of host-independent T7 expression system (HITES) for a broad host range	11
A simple and efficient biodegradation route for nitroaromatic compounds using a nitroreductase from Bacillus subtilis and its “cell-like” immobilized form	11
A review on anticancer, antibacterial and photo catalytic activity of various nanoparticles synthesized by probiotics	11
Optimizing mycelial protein yield in Pleurotus djamor via ARTP mutagenesis and hybridization strategies	11
Optimization of fungal fermentation for the extraction of polyphenols from Flourensia cernua and its effect on cellular metabolism	11
Editorial Board	11
Investigation of anti-adherence and antimicrobial properties of prodigiosin-functionalized bacterial cellulose membrane for biomedical applications	11
Production and purification of recombinant long protein isoforms of FGF11 subfamily	11
Improved biocatalytic activity of steapsin lipase in supercritical carbon dioxide medium for the synthesis of benzyl butyrate: A commercially important flavour compound	11
Characterization of 3D-printed acrylonitrile butadiene styrene and polylactic acid carrier-beads for Gluconobacter oxydans immobilization	11
Human beta-defensin 3-functionalized Fe/GMP nanozyme for multifunctional antimicrobial and anticancer activity against Helicobacter pylori-associated gastrointestinal cancer	11
Editorial Board	11
Volatile organic compounds: A threat to the environment and health hazards to living organisms – A review	11
Molecular biomarkers identification and applications in CHO bioprocessing	10
Fuel characterization, engine performance characteristics and emissions analysis of different mustard seed biodiesel: An overview	10
Advancement on mixed microalgal-bacterial cultivation systems for nitrogen and phosphorus recoveries from wastewater to promote sustainable bioeconomy	10

Cell-based shear stress sensor for bioprocessing	10
Immobilization of aldoxime dehydratases on metal affinity resins and use of the immobilized catalysts for the synthesis of nitriles important in fragrance industry	10
High-concentration Mg2+ stress improves L-lactic acid biosynthesis of Bacillus coagulans revealed by combined analysis of transcriptome and metabolome	10
Increasing the chiral purity of cellulosic L-lactic acid by D-Lactate oxidase-catalyzed oxidation in engineered lactic acid bacterium Pediococcus acidilactici	10
Editorial Board	10
Synergistic interaction of AaMYC2 and AaMYC2-LIKE enhances artemisinin production in Artemisia annua L.	10
Evaluating the role of trypsin in silk degumming: An in silico approach	10
Impact of liquid and solid-state cultures on hemoglobin production and oxidative state in Saccharomyces cerevisiae	10
Engineering the cell wall reactive groups of Plant Growth Promoting Rhizobacteria by culture strategy for heavy metal removal	10
A pump-free microfluidic co-culture system for investigating NK cell-tumor spheroid interactions in flow conditions	10
Modular engineering of the doxorubicin biosynthetic gene cluster based on component functional clustering in Streptomyces	10
Efficient expression and functional verification of recombinant human type Ⅲ collagen in CHO cells	10
Development of a 10 g/L process for a difficult-to-express multispecific antibody format using a holistic process development approach	10
A highly efficient mixed strain fermentation strategy to produce 11α-Hydroxyandrost-4-ene-3,17-dione from phytosterols	10
Comparison of plant biostimulating properties of Chlorella sorokiniana biomass produced in batch and semi-continuous systems supplemented with pig manure or acetate	10
Editorial Board	10
Bone marrow-derived dendritic cells play a role in attenuating inflammation on Bothrops jararacussu venom muscle damage	10
Multimetallic biochar as an ecosystem engineer: Orchestrating synergistic IHT-DIET pathways via spatial niche partitioning for enhanced anaerobic digestion	10
Virion-surface display of a chimeric immunoglobulin Fc domain facilitating uptake by antigen-presenting cells	10
Reduced production of Ethyl Carbamate in wine by regulating the accumulation of arginine in Saccharomyces cerevisiae	10
Enhancing the amination activity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum by modifying the crucial residue His154 for deamination	10
Engineering adaptive alleles for Escherichia coli growth on sucrose using the EasyGuide CRISPR system	9
High yield secretion of human erythropoietin from tobacco cells for ex vivo differentiation of hematopoietic stem cells towards red blood cells	9
De novo biosynthesis of lavandulol and lavandulyl acetate in Escherichia coli	9
Structure-guided engineering of 4-coumarate: CoA ligase for efficient production of rosmarinic acid in Saccharomyces cerevisiae	9
Biotechnological parameters of cultivation of mycorrhizal fungi Cortinarius caperatus and Laccaria bicolor	9
Microalgae as a potential therapeutic drug candidate for neurodegenerative diseases	9
Sortase E-mediated site-specific immobilization of green fluorescent protein and xylose dehydrogenase on gold nanoparticles	9
Endophyte-assisted non-host plant Tillandsia brachycaulos enhance indoor formaldehyde removal	9
CRISPR-based precise methylation of specific FUT8 promoter regions allows isolation of CHO cells with a fine-tuned glycoprofile	9
System-wide analysis of groundnut's salinity resilience: Integrating plant-cell interactions with environmental stress dynamics through cutting-edge transcriptomics	9
Glycolate as alternative carbon source for Escherichia coli	9
Engineered L-phenylserine aldolase enhances L-norvaline synthesis within an enzyme cascade	9
Optical, structural, and antifungal properties of nanosilver borate bioactive glass synthesized using gamma rays on the survival of Candida albicans and Candida tropicalis	9
Interferon inhibitors increase rAAV production in HEK293 cells	9
Ectoine production from a novel bacterial strain and high-purity purification with a cost-effective and single-step method	9
Editorial Board	8
Inhibition of tau phosphorylation and Aβ accumulation by S. cerevisiae-derived vacuoles in LPS-induced SH-SY5Y cells	8
Identification and characterization of anaerobically activated promoters in Escherichia coli	8
Enhancement of production of glycoalkaloids by elicitors along with characterization of gene expression of pathways in Solanum xanthocarpum	8
Editorial Board	8
Metabolic engineering of Escherichia coli for seleno-methylselenocysteine production	8
Streamlined in vitro screening system of synthetic signal peptides in Chinese hamster ovary cells for therapeutic protein production	8
RNA-based detection of genetically modified plants via current-voltage characteristic measurement	8
Physiochemical and micro-morphological response for four bloom-forming algal strains under phosphite (P+III) stress and phosphate (P⁺ⱽ) recovery	8
pH-driven metabolic reprogramming in Bacillus velezensis 83 regulates metabolite synthesis and sporulation: A transcriptional approach for bioprocess development	8
Database-driven in silico-identification and characterization of novel aldoxime dehydratases	8
Identification and structure-based engineering of a dipeptidase CpPepD from Clostridium perfringens for the synthesis of l-carnosine	8
Metabolic engineering of carotenoid biosynthesis in Yarrowia lipolytica	8
Uncovering gene expression signatures and diagnostic – Biomarkers in hepatocellular carcinoma through multinomial logistic regression analysis	8
A robust and efficient alluvial filtration method for the clarification of adeno-associated viruses from crude cell lysates	8
Immobilization of glycosyltransferase into a hydrophilic metal-organic framework for efficient biosynthesis of chondroitin sulfate	8
Unlocking new frontiers in healthcare: The impact of nano-optical biosensors on personalized medical diagnostics	8
Optimizing bio-vanillin synthesis from ferulic acid via Pediococcus acidilactici: A systematic approach to process enhancement and yield maximization	8
Enhanced prokaryotic expression, purification, and biological activities of human keratinocyte growth factor	8
Regulatory non-coding RNA: The core defense mechanism against plant pathogens	8
Exploring the substrate scope of glycerol dehydrogenase GldA from E. coli BW25113 towards cis-dihydrocatechol derivatives	8
Multifactorial optimization enables the identification of a greener method to produce (+)-nootkatone	8
Efficient multiplex CRISPR/Cpf1 (Cas12a) genome editing system in Aspergillus aculeatus TBRC 277	8
Multi-parameter process optimization for high yield AAV2 vector production using scale-down multi-parallel bioreactor systems and high-throughput analytical tools	8
A chitosan-integrated antibacterial protein composite nanocomplex derived from barnacle cement and spider silk	8
Editorial Board	8
Efficient rDNA-mediated multi-copy integration of gene clusters in Aureobasidium melanogenum	8
Advances in photonic crystal hydrogels for biomedical research: A review	8
N-region of Cry1Ia: A novel fusion tag for Escherichia coli and Pichia pastoris	8
Metabolic engineering and high-cell-density fermentation of Corynebacterium glutamicum to improve isopropanol production	8