Trends in Biotechnology

Article	Citations
Subscription and Copyright information	201
Advisory Board and Contents	185
Phytohormones: novel strategy for removing emerging contaminants and recovering resources	167
Bridging the path to multiplexed molecular diagnostics	166
Microalgae: a revolution for salt-affected soil remediation	163
Electronic plants: the future of agriculture and urban ecosystems?	162
The green revolution of food waste upcycling to produce polyhydroxyalkanoates	160
Scalable tissue biofabrication via perfusable hollow fiber arrays for cultured meat applications	131
Analyzing and understanding the robustness of bioprocesses	129
CRISPR-based technologies for large DNA insertions	128
Smart materials: rational design in biosystems via artificial intelligence	128
SynMADE: synthetic microbiota across diverse ecosystems	122
Healing wounds with plasma-activated hydrogel therapy	116
Vector-free intra-airway in vivo epigenetic editing	115
Cofactor engineering powers next-generation biomanufacturing	112
Auricular repair: symphony between scaffolds and stem cells	103
Unlocking the potential of stem-cell-derived ‘synthetic’ embryo models	102
Manipulating immune activity of macrophages: a materials and mechanics perspective	97
Engineering biology approaches to modulate bacterial biofilms	94
Prime editing: advances and therapeutic applications	87
Advisory Board and Contents	83
Next-generation digital biomarkers: continuous molecular health monitoring using wearable devices	82
Subscription and Copyright information	82
Manufacturing innovation to drive down cell therapy costs	81
Subscription and Copyright information	81

UFSC Biotech Network: a transdisciplinary platform for biotechnology innovation in Brazil	81
Subscription and Copyright information	81
Advisory Board and Contents	80
Integrating bicarbonate-based microalgal production with alkaline sewage for ocean negative carbon emissions	79
Patentability of antibodies in the artificial intelligence era	77
Mini-bones: miniaturized bone in vitro models	77
Subscription and Copyright information	77
Printhead on a chip: empowering droplet-based bioprinting with microfluidics	75
A high-performance protein preparation approach in a single column-free step	75
Beyond waste: understanding urine’s potential in precision medicine	74
Biotechnologies based on the fungal bioluminescence pathway	74
The GPS for drug development: navigating with evidence from human populations	73
Influence of titer, rate, yield, and scale on the cost and life-cycle emissions of biomanufacturing	72
Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia	72
Molecular design of microalgae as sustainable cell factories	72
Ocular RNA nanomedicine: engineered delivery nanoplatforms in treating eye diseases	71
Nanobotic microbiomes against emerging vascular pathogens in agriculture	71
Emerging strategies to develop novel genetically encoded biosensors	71
Towards biomanufacturing of cultured meat	71
Extended human lymph node explants for evaluation of adaptive immunity	70
Translational organoid technology – the convergence of chemical, mechanical, and computational biology	69
Can protein expression be ‘solved’?	68
Machine learning to predict de novo protein–protein interactions	67
Machine learning in bioprocess development: from promise to practice	66
Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis	66
Next-generation 3D-printed nutritious food derived from waste plastic and biomass	65
Advisory Board and Contents	65
Subscription and Copyright information	64
Advisory Board and Contents	63
The LEGO® brick road to open science and biotechnology	63
Advisory Board and Contents	62
Biosafety and biosecurity: treading China’s synbio tightrope	62
Advisory Board and Contents	62
Sebum analysis as an advantageous source of biomolecular information	62
Advances in lung biomimetic systems: exploring biophysical cues in lung regenerative medicine	60
Emerging supramolecular and living materials in oral medicine	59
Frog ‘N’ Roll: novel synthetic peptides selective against Gram-negative pathogens	59
Harnessing Streptomyces–plant interactions for agricultural natural product discovery	56
Dynamically regulating metabolic fluxes with synthetic metabolons	55
Subscription and Copyright information	55
Building biomaterials through genetic code expansion	54
Improving awareness, understanding, and enforcement of responsibilities and regulations in Blue Biotechnology	54
A new chapter for anti-idiotypes in low molecular weight compound immunoassays	54
Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions	53
Dynamic plasmid copy number control for synthetic biology	53
The morally disruptive future of reprogenetic enhancement technologies	53
Multienzymatic synthesis of nylon monomers from vegetable oils	52
Small but mighty: emerging roles of uORFs in plants	52
Engineered bacterial therapeutics with material solutions	51
Ethical challenges with 3D bioprinted tissues and organs	51

Advisory Board and Contents	51
Using synthetic biology to express nitrogenase biosynthesis pathway in rice and to overcome barriers of nitrogenase instability in plant cytosol	50
3D printing of heart valves	50
Estimating environmental impacts of early-stage bioprocesses	49
Metabolic and immunomodulatory control of type 2 diabetes via generating cellular itaconate reservoirs by inflammatory-targeting gene-therapy nanovesicles	49
Nanobiomaterials to modulate natural killer cell responses for effective cancer immunotherapy	47
Bioprinted, spatially defined breast tumor microenvironment models of intratumoral heterogeneity and drug resistance	47
Trends in sustainable single-cell protein from non-grain feedstocks	46
Biotechnological approaches for producing natural pigments in yeasts	45
Synthetic biology for plant genetic engineering and molecular farming	45
Design principles for engineering light-controlled antibodies	45
Spatial omics for accelerating plant research and crop improvement	45
3D extrusion bioprinting: rational bioink design and advanced fabrication techniques	45
Rethinking techno-moral disruption in bioethics, society, and justice	44
Molecular engineering of insulin for recombinant expression in yeast	44
From academia to real-life biotech: ‘It takes a village’	44
Protein–glycan engineering in vaccine design: merging immune mechanisms with biotechnological innovation	44
Academic entrepreneurship: promise and perils	43
One-pot production of colored bacterial cellulose	42
Innovating biomanufacturing with coculture-based engineered living materials	41
Rapid selection of transgenic mammalian cells via diphtheria toxin resistance	41
Next-generation industrial biotechnology for low-cost mass production of PHA	41
Therapeutic microneedles for myocardial repair	41
One-pot expression of an oligoclonal nanobody-based antivenom in growth-decoupled Escherichia coli	41
Non-sterile cultivation of oleaginous organisms	40
Solar-driven PHB synthesis from wastewater by engineered semiconductor–bacteria biohybrids	40
Breaking the immunosuppressive barrier: an armored CAR-M biotechnology with the original M1 phenotype dominance rescues antitumor immunity	40
Microbial electrosynthesis: opportunities for microbial pure cultures	39
Advances in induced anhydrobiosis for cell and gamete storage	39
Advanced strategies for enzyme–electrode interfacing in bioelectrocatalytic systems	39
Robots with a human touch	38
Subscription and Copyright information	38
Making cow-free caseins and casein micelles	38
Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion	38
Subscription and Copyright information	38
Marine-derived bioactive materials as antibiofilm and antivirulence agents	37
CRISPR screen-based mammalian cell engineering for complex biotherapeutics	37
Microfluidic-based skin-on-chip systems for safety assessment of nanomaterials	36
Advisory Board and Contents	36
Nanobodies: a new frontier in plant disease management	35
Bioprocessing technology of muscle stem cells: implications for cultured meat	35
Biosensor-guided indexing of genome evolution for boosting microbial chemical synthesis	35
Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration	34
HHV-6 and HHV-7 reactivation in allogeneic CAR-T cell therapy	34
Computationally guided genome rewiring of Escherichia coli and its application for nanopolyethylene terephthalate (PET) biodegradation and upcycling	34
Harnessing TLS polymerases to direct nucleotide outcomes in base editing	34
Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes	34
CRISPR-Cas12c: a noncleaving DNA binder with minimal PAM requirement	33
Exploring standards for multicellular mammalian synthetic biology	33
Harnessing synthetic biology to develop novel psychedelic therapies	33
Virus-like particles as modular interfaces for biomaterial functionalization	33
How transdisciplinarity can help biotech-driven biodiversity research	33
Epicardial patches for myocardial repair: circumventing barriers to heal ischemic heart tissue	33
Welfare impacts of China's regulatory change toward genome-edited crops	32
Transforming undergraduate laboratory courses with interlinked real-world challenges	32
Efficient and sustained photosynthetic hydrogen production by algae under high light intensity	32
An epigenetic regulator synergizes with alphavirus-mediated gene therapy via biomimetic delivery for enhanced cancer therapy	32
Clinical technology advances driving in vivo CAR therapy	31
Biotechnology applications of proteins functionalized with DNA oligonucleotides	31
Novel delivery systems for controlled release of bacterial therapeutics	31
Light-induced programmable solid–liquid phase transition of biomolecular condensates for improved biosynthesis	31
Organ-on-a-chip systems for modeling tumor and normal tissue microenvironments in radiotherapy research	31
Distillation for in situ recovery of volatile fermentation products	30
Engineering M13 phages: surface and morphological strategies for programmable applications	30
Thermotolerant yeasts and their biotechnological applications	30
The FDA’s plan to phase out animal testing	30
Engineered RNA nanostructures for scalable and efficient RNAi-based pesticides	30
pHNRhCas9NG, single expression cassette-based dual-component dual-transcription unit CRISPR/Cas9 system for plant genome editing	30
CRISPR/Cas-based personal glucose meters for nucleic acid detection	29
Harnessing the microbial world for human benefit	29
Product-driven high-throughput screening of industrial filamentous actinomycetes	29
Immunostimulation in experimental gas plasma therapy for breast cancer	29
Harnessing piezoelectricity for musculoskeletal regeneration: microcurrents to tissue repair	28
Advancing plant gene activation with CRISPR-Act3.0	28
Ethical and legal considerations of digital animal models: pioneering reduction and replacement	27
Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production	27
Application of bacterial extracellular vesicles in gastrointestinal diseases	27
Prime editor integrase systems boost targeted DNA insertion and beyond	27
Gas plasma technology mediates deep tissue and anticancer events independently of hydrogen peroxide	27
Synthetic M13 phage engagers expand CAR-T cell antigen recognition to overcome tumor heterogeneity	27

Chips, guts, and gas: unraveling volatile microbial mysteries in real time!	27
Rewiring holobiont systems with synthetic biology	27
RNA and nanocarriers: next generation drug and delivery platform take center stage	27
Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering	26
Dynamic anaerobic digestion-based biorefineries for on-demand renewable energy and bioproducts in a circular bioeconomy	26
Detection of anti-drug antibodies during monoclonal antibody drug therapy	26
Rational bioengineering of polysaccharide in designing of microbiome modulation	26
Phase-separated biomolecular condensates for biocatalysis	26
Evading and overcoming AAV neutralization in gene therapy	25
Subscription and Copyright information	25
Deep learning-guided discovery and engineering of binding peptides for accelerated enzymatic degradation of polyethylene terephthalate	25
OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window	25
Synthetic transcription factor engineering for cell and gene therapy	25
Duckweed biomanufacturing: longstanding promise, decade-long lag, recent renaissance	25
Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation	25
Next-generation electroactive biomaterials-integrated bioelectronics for advanced healthcare management	24
Advancing wheat breeding using rye: a key contribution to wheat breeding history	24
Prokaryotic Argonautes for in vivo biotechnology and molecular diagnostics	24
Bioinspired thermoreversible bioink orchestrates focal adhesion-dependent osteogenesis	24
The lignocellulosic biorefinery concept is sound: a commentary on Zhao et al.	24
Shaping the future US bioeconomy through safety, security, sustainability, and social responsibility	24
Yeast-based screening platforms to understand and improve human health	24
Advisory Board and Contents	24
Integrated directed evolution and semirational design of Pyrococcus furiosus Argonaute for ultrasensitive tetracycline detection	24
Advancements and challenges in CAR-NK cell therapy for cancer treatment	24
Recent advances in systems engineering approaches to integrated strain and bioprocess development	23
Framing synthetic biology as an innovation-driven market	23
Comparison of CRISPR/Cas and Argonaute for nucleic acid tests	23
Editing a healthier future in plants	23
Hydrogen production in microbial electrolysis cells with biocathodes	23
Bioart and biosafety: navigating ‘living art’ within the European regulatory framework	23
Power-to-vitamins: producing folate (vitamin B9) from renewable electric power and CO2 with a microbial protein system	23
Artificial intelligence in biomaterials: a call for unified biocompatibility standards	22
Robustness: linking strain design to viable bioprocesses	22
Chimeric antigen receptor therapy meets mRNA technology	22
Intervertebral disc-on-a-chip: a precision engineered toolbox for low back pain studies	22
Bringing carbon to life via one-carbon metabolism	22
The biotechnology revolution in textile dyeing	22
Advancements and regulations of biomanufacturing cell-based cartilage repair therapies	22
Human bone marrow organoids: emerging progress but persisting challenges	22
Escherichia coli selection strains for growth-coupled metabolic engineering	22
Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions	22
Reimagining colour with synthetic biology	21
Pollutant degradation in saline wastewater using synthetic biology	21
Large language model for knowledge synthesis and AI-enhanced biomanufacturing	21
Subscription and Copyright information	21
Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapy	21
Cellular reprogramming and the rise of rejuvenation biotech	21
Media representations of synthetic biology in China	21
Cadherin-engineered microspheres enable scalable biofabrication of multilineage liver organoids	21
Towards machine learning for hydrogel drug delivery systems	21
Large-scale manufacturing of human gallbladder epithelial cell products and derived hepatocytes via a chemically defined approach	21
Subscription and Copyright information	21
Insights on cisgenic plants with durable disease resistance under the European Green Deal	20
One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives	20
Companies: share cultivated meat breakthroughs publicly—here is how	20
Efficiency and process development for microbial biomass production using oxic bioelectrosynthesis	20
New technologies for production of recombinant spider silk	20
Navigating microalgae under dynamic CO2 for waste-to-energy conversion	20
Strengthening the competitiveness of EU in vitro biotechnologies	20
Untethered: using remote magnetic fields for regenerative medicine	20
Implantable vascularized endocrine constructs for clinically scalable insulin delivery	20
Synthetic biology for Taxol biosynthesis and sustainable production	20
Engineering chimeric signaling proteins for microbial whole-cell biosensors: from design to deployment	20
Microbial cellulose production using ocean water: sustainable and cost-effective?	20
Harnessing microbes to pioneer environmental biophotoelectrochemistry	19
Subscription and Copyright information	19
Spatial -omics technologies: the new enterprise in 3D breast cancer models	19
Subscription and Copyright information	19
A pathway to improve seaweed aquaculture through microbiota manipulation	19
Advances in ultrahigh-throughput screening technologies for protein evolution	19
Point-of-care detection devices for wound care and monitoring	19
Organs-on-chips: a decade of innovation	19
A forward-thinking biocompatibility assessment: drafting future considerations now	19
Resilience-by-design in engineering Bacillus cell factories	19
Establishment of a nonhomologous end joining-mediated genomic library in Fusarium fujikuroi for high-level production of gibberellic acid	19
Bioprinting is not cheap	18
Plant-based models of neurodegenerative CAG repeat expansion diseases	18
Holistic ecosystem strategies for boosting biopharma start-up success	18
Engineering next-generation oxygen-generating scaffolds to enhance bone regeneration	18
Robotic micromotors transforming oral drug administration	18
Designing at-source and end-of-pipe biotechnologies to tackle pharmaceutical pollution	18
Gene drive in species complexes: defining target organisms	18
CarveAdornCurate: a versatile cloud-based platform for constructing multiscale metabolic models	18
Protein–nucleic acid thermodynamic databases for specific uses	18
Quality by Design for enabling RNA platform production processes	17
Innovation trends in industrial biotechnology	17
Biosensors for healthcare: current and future perspectives	17
Agricultural biotechnology for sustainable food security	17
Ethics and engagement: steering China’s synbio future	17
Driving towards digital biomanufacturing by CHO genome-scale models	17
Design of biomass-based renewable materials for environmental remediation	17
EccDNA in plant-stress and biotechnological solutions in agriculture	17
A ventilated perfused lung model platform to dissect the response of the lungs to viral infection	17
TRYing to evaluate production costs in microbial biotechnology	17