Trends in Biotechnology

Article	Citations
Subscription and Copyright information	214
Advisory Board and Contents	184
Bridging the path to multiplexed molecular diagnostics	182
Electronic plants: the future of agriculture and urban ecosystems?	178
CRISPR-based technologies for large DNA insertions	170
SynMADE: synthetic microbiota across diverse ecosystems	167
Engineering biology approaches to modulate bacterial biofilms	146
Unlocking the potential of stem-cell-derived ‘synthetic’ embryo models	141
Healing wounds with plasma-activated hydrogel therapy	140
Auricular repair: symphony between scaffolds and stem cells	138
Microalgae: a revolution for salt-affected soil remediation	133
Smart materials: rational design in biosystems via artificial intelligence	120
Scalable tissue biofabrication via perfusable hollow fiber arrays for cultured meat applications	116
Phytohormones: novel strategy for removing emerging contaminants and recovering resources	112
Prime editing: advances and therapeutic applications	111
Cofactor engineering powers next-generation biomanufacturing	103
The green revolution of food waste upcycling to produce polyhydroxyalkanoates	99
Manipulating immune activity of macrophages: a materials and mechanics perspective	98
Vector-free intra-airway in vivo epigenetic editing	94
Analyzing and understanding the robustness of bioprocesses	92
Advisory Board and Contents	91
Subscription and Copyright information	89
Subscription and Copyright information	87
Subscription and Copyright information	85
Advisory Board and Contents	84

UFSC Biotech Network: a transdisciplinary platform for biotechnology innovation in Brazil	84
Subscription and Copyright information	83
A high-performance protein preparation approach in a single column-free step	83
Towards biomanufacturing of cultured meat	82
J. Craig Venter (1946—2026)	81
Extended human lymph node explants for evaluation of adaptive immunity	81
Integrating bicarbonate-based microalgal production with alkaline sewage for ocean negative carbon emissions	80
Beyond waste: understanding urine’s potential in precision medicine	80
Ocular RNA nanomedicine: engineered delivery nanoplatforms in treating eye diseases	78
Influence of titer, rate, yield, and scale on the cost and life-cycle emissions of biomanufacturing	78
Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis	77
Engineering environmental bacteria for whole-cell PET hydrolysis and assimilation	76
Nanobotic microbiomes against emerging vascular pathogens in agriculture	76
Next-generation digital biomarkers: continuous molecular health monitoring using wearable devices	76
Emerging strategies to develop novel genetically encoded biosensors	75
Molecular design of microalgae as sustainable cell factories	75
Machine learning to predict de novo protein–protein interactions	75
The GPS for drug development: navigating with evidence from human populations	73
Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia	72
Engineering single-dose plasmid DNA for sustained in vivo delivery of designer incretins	72
Biotechnologies based on the fungal bioluminescence pathway	71
Patentability of antibodies in the artificial intelligence era	71
Printhead on a chip: empowering droplet-based bioprinting with microfluidics	69
Can protein expression be ‘solved’?	68
Translational organoid technology – the convergence of chemical, mechanical, and computational biology	67
Machine learning in bioprocess development: from promise to practice	66
Manufacturing innovation to drive down cell therapy costs	66
Mini-bones: miniaturized bone in vitro models	66
Metabolic and immunomodulatory control of type 2 diabetes via generating cellular itaconate reservoirs by inflammatory-targeting gene-therapy nanovesicles	64
Advisory Board and Contents	64
Estimating environmental impacts of early-stage bioprocesses	63
Dynamically regulating metabolic fluxes with synthetic metabolons	62
Engineered bacterial therapeutics with material solutions	61
Improving awareness, understanding, and enforcement of responsibilities and regulations in Blue Biotechnology	61
The LEGO® brick road to open science and biotechnology	58
Using synthetic biology to express nitrogenase biosynthesis pathway in rice and to overcome barriers of nitrogenase instability in plant cytosol	57
Advisory Board and Contents	56
Dynamic plasmid copy number control for synthetic biology	56
Advisory Board and Contents	56
Advisory Board and Contents	56
Advisory Board and Contents	56
Frog ‘N’ Roll: novel synthetic peptides selective against Gram-negative pathogens	55
Biosafety and biosecurity: treading China’s synbio tightrope	53
A new chapter for anti-idiotypes in low molecular weight compound immunoassays	53
Subscription and Copyright information	52
Nanobiomaterials to modulate natural killer cell responses for effective cancer immunotherapy	51
Small but mighty: emerging roles of uORFs in plants	51
Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions	51
Multienzymatic synthesis of nylon monomers from vegetable oils	50
Sebum analysis as an advantageous source of biomolecular information	49

Emerging supramolecular and living materials in oral medicine	49
Harnessing Streptomyces–plant interactions for agricultural natural product discovery	49
The morally disruptive future of reprogenetic enhancement technologies	49
Advances in lung biomimetic systems: exploring biophysical cues in lung regenerative medicine	48
Trends in sustainable single-cell protein from non-grain feedstocks	48
Next-generation 3D-printed nutritious food derived from waste plastic and biomass	48
3D printing of heart valves	47
3D extrusion bioprinting: rational bioink design and advanced fabrication techniques	47
Spatial omics for accelerating plant research and crop improvement	47
Bioprinted, spatially defined breast tumor microenvironment models of intratumoral heterogeneity and drug resistance	46
Building biomaterials through genetic code expansion	46
Synthetic biology for plant genetic engineering and molecular farming	46
Ethical challenges with 3D bioprinted tissues and organs	46
Design principles for engineering light-controlled antibodies	45
Responsible biotechnology: an analysis of the Asilomar 2025 entreaties	45
Rethinking techno-moral disruption in bioethics, society, and justice	44
From academia to real-life biotech: ‘It takes a village’	44
Academic entrepreneurship: promise and perils	44
Non-sterile cultivation of oleaginous organisms	44
Next-generation industrial biotechnology for low-cost mass production of PHA	43
Rapid selection of transgenic mammalian cells via diphtheria toxin resistance	43
Solar-driven PHB synthesis from wastewater by engineered semiconductor–bacteria biohybrids	42
Multiscale and programmable engineering of edible mushroom mycelium-based materials	42
Breaking the immunosuppressive barrier: an armored CAR-M biotechnology with the original M1 phenotype dominance rescues antitumor immunity	42
Biotechnological approaches for producing natural pigments in yeasts	42
One-pot expression of an oligoclonal nanobody-based antivenom in growth-decoupled Escherichia coli	41
One-pot production of colored bacterial cellulose	41
Microbial electrosynthesis: opportunities for microbial pure cultures	39
Therapeutic microneedles for myocardial repair	39
Protein–glycan engineering in vaccine design: merging immune mechanisms with biotechnological innovation	39
Advanced strategies for enzyme–electrode interfacing in bioelectrocatalytic systems	38
Transporter engineering in microbial cell factories	38
Molecular engineering of insulin for recombinant expression in yeast	38
Innovating biomanufacturing with coculture-based engineered living materials	38
Advances in induced anhydrobiosis for cell and gamete storage	38
Reprogrammable bacterial nanosyringes to deliver RNA and gene editors	37
Engineering energy-robust Clostridium autoethanogenum via integrated nanotechnology and transcription-guided metabolic reprogramming	37
Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion	36
Robots with a human touch	36
How transdisciplinarity can help biotech-driven biodiversity research	36
Subscription and Copyright information	36
Subscription and Copyright information	36
Making cow-free caseins and casein micelles	36
Harnessing TLS polymerases to direct nucleotide outcomes in base editing	35
HHV-6 and HHV-7 reactivation in allogeneic CAR-T cell therapy	35
Advisory Board and Contents	35
Nanobodies: a new frontier in plant disease management	35
Microfluidic-based skin-on-chip systems for safety assessment of nanomaterials	35
Biosensor-guided indexing of genome evolution for boosting microbial chemical synthesis	34
Epicardial patches for myocardial repair: circumventing barriers to heal ischemic heart tissue	34
CRISPR screen-based mammalian cell engineering for complex biotherapeutics	34
CRISPR-Cas12c: a noncleaving DNA binder with minimal PAM requirement	33
Marine-derived bioactive materials as antibiofilm and antivirulence agents	33
Novel delivery systems for controlled release of bacterial therapeutics	33
Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes	33
Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration	33
Biotechnology applications of proteins functionalized with DNA oligonucleotides	33
Computationally guided genome rewiring of Escherichia coli and its application for nanopolyethylene terephthalate (PET) biodegradation and upcycling	33
Harnessing synthetic biology to develop novel psychedelic therapies	32
Exploring standards for multicellular mammalian synthetic biology	32
Welfare impacts of China's regulatory change toward genome-edited crops	32
Transforming undergraduate laboratory courses with interlinked real-world challenges	31
Product-driven high-throughput screening of industrial filamentous actinomycetes	30
CRISPR/Cas-based personal glucose meters for nucleic acid detection	30
Engineered RNA nanostructures for scalable and efficient RNAi-based pesticides	30
pHNRhCas9NG, single expression cassette-based dual-component dual-transcription unit CRISPR/Cas9 system for plant genome editing	30
Efficient and sustained photosynthetic hydrogen production by algae under high light intensity	30
Light-induced programmable solid–liquid phase transition of biomolecular condensates for improved biosynthesis	29
Advisory Board and Contents	29
An epigenetic regulator synergizes with alphavirus-mediated gene therapy via biomimetic delivery for enhanced cancer therapy	29
Distillation for in situ recovery of volatile fermentation products	29
Thermotolerant yeasts and their biotechnological applications	29
Engineered photothermal-responsive bacterial platform for in vivo fluorescence imaging-guided photothermal/gene combined breast cancer therapy	29
The FDA’s plan to phase out animal testing	29
Immunostimulation in experimental gas plasma therapy for breast cancer	29
Clinical technology advances driving in vivo CAR therapy	29
Organ-on-a-chip systems for modeling tumor and normal tissue microenvironments in radiotherapy research	29
Engineering M13 phages: surface and morphological strategies for programmable applications	29
Driving the future of proteome-based innovations: the π-HuB project	29
Virus-like particles as modular interfaces for biomaterial functionalization	29

Advancing plant gene activation with CRISPR-Act3.0	28
Engineered Bacillus subtilis to deliver dsRNA via extracellular vesicles against the H9N2 avian influenza virus	28
Application of bacterial extracellular vesicles in gastrointestinal diseases	28
Ethical and legal considerations of digital animal models: pioneering reduction and replacement	28
Chips, guts, and gas: unraveling volatile microbial mysteries in real time!	28
Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering	28
RNA and nanocarriers: next generation drug and delivery platform take center stage	28
Harnessing the microbial world for human benefit	28
Rewiring holobiont systems with synthetic biology	28
Deep learning-guided discovery and engineering of binding peptides for accelerated enzymatic degradation of polyethylene terephthalate	28
Evading and overcoming AAV neutralization in gene therapy	27
Synthetic transcription factor engineering for cell and gene therapy	27
Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation	27
OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window	27
Detection of anti-drug antibodies during monoclonal antibody drug therapy	27
Synthetic M13 phage engagers expand CAR-T cell antigen recognition to overcome tumor heterogeneity	26
Duckweed biomanufacturing: longstanding promise, decade-long lag, recent renaissance	26
Harnessing piezoelectricity for musculoskeletal regeneration: microcurrents to tissue repair	26
Prime editor integrase systems boost targeted DNA insertion and beyond	26
Gas plasma technology mediates deep tissue and anticancer events independently of hydrogen peroxide	26
The oriGen case and Mexico’s regulatory blind spots in genomic biobanking	26
Dynamic anaerobic digestion-based biorefineries for on-demand renewable energy and bioproducts in a circular bioeconomy	26
Rational bioengineering of polysaccharide in designing of microbiome modulation	25
Intervertebral disc-on-a-chip: a precision engineered toolbox for low back pain studies	25
Phase-separated biomolecular condensates for biocatalysis	25
Subscription and Copyright information	25
Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production	25
Artificial intelligence in biomaterials: a call for unified biocompatibility standards	25
Advisory Board and Contents	24
Advancements and challenges in CAR-NK cell therapy for cancer treatment	24
Editing a healthier future in plants	24
Bioart and biosafety: navigating ‘living art’ within the European regulatory framework	24
The lignocellulosic biorefinery concept is sound: a commentary on Zhao et al.	24
Shaping the future US bioeconomy through safety, security, sustainability, and social responsibility	24
Framing synthetic biology as an innovation-driven market	24
Yeast-based screening platforms to understand and improve human health	24
Recent advances in systems engineering approaches to integrated strain and bioprocess development	24
The biotechnology revolution in textile dyeing	24
Human bone marrow organoids: emerging progress but persisting challenges	23
Advancing wheat breeding using rye: a key contribution to wheat breeding history	23
Next-generation electroactive biomaterials-integrated bioelectronics for advanced healthcare management	23
Bringing carbon to life via one-carbon metabolism	23
Hydrogen production in microbial electrolysis cells with biocathodes	23
Chimeric antigen receptor therapy meets mRNA technology	23
Prokaryotic Argonautes for in vivo biotechnology and molecular diagnostics	23
Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions	23
Power-to-vitamins: producing folate (vitamin B9) from renewable electric power and CO2 with a microbial protein system	23
Comparison of CRISPR/Cas and Argonaute for nucleic acid tests	23
Escherichia coli selection strains for growth-coupled metabolic engineering	23
Integrated directed evolution and semirational design of Pyrococcus furiosus Argonaute for ultrasensitive tetracycline detection	23
Bioinspired thermoreversible bioink orchestrates focal adhesion-dependent osteogenesis	22
Robustness: linking strain design to viable bioprocesses	22
Synthetic biology for Taxol biosynthesis and sustainable production	22
Advancements and regulations of biomanufacturing cell-based cartilage repair therapies	22
Reimagining colour with synthetic biology	22
Cadherin-engineered microspheres enable scalable biofabrication of multilineage liver organoids	22
Large language model for knowledge synthesis and AI-enhanced biomanufacturing	22
Media representations of synthetic biology in China	22
Subscription and Copyright information	21
New technologies for production of recombinant spider silk	21
Pollutant degradation in saline wastewater using synthetic biology	21
Insights on cisgenic plants with durable disease resistance under the European Green Deal	21
Subscription and Copyright information	21
Companies: share cultivated meat breakthroughs publicly—here is how	21
Strengthening the competitiveness of EU in vitro biotechnologies	21
One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives	20
Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapy	20
Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles	20
Subscription and Copyright information	20
A ventilated perfused lung model platform to dissect the response of the lungs to viral infection	20
Navigating microalgae under dynamic CO2 for waste-to-energy conversion	20
Towards machine learning for hydrogel drug delivery systems	20
Organs-on-chips: a decade of innovation	20
Resilience-by-design in engineering Bacillus cell factories	20
Microbial cellulose production using ocean water: sustainable and cost-effective?	20
Large-scale manufacturing of human gallbladder epithelial cell products and derived hepatocytes via a chemically defined approach	20
Implantable vascularized endocrine constructs for clinically scalable insulin delivery	20
Engineering chimeric signaling proteins for microbial whole-cell biosensors: from design to deployment	20
Subscription and Copyright information	20
Point-of-care detection devices for wound care and monitoring	20
Untethered: using remote magnetic fields for regenerative medicine	19
Bioprinting is not cheap	19
EccDNA in plant-stress and biotechnological solutions in agriculture	19
Spatial -omics technologies: the new enterprise in 3D breast cancer models	19
Plant-based models of neurodegenerative CAG repeat expansion diseases	19
Designing at-source and end-of-pipe biotechnologies to tackle pharmaceutical pollution	19
Protein–nucleic acid thermodynamic databases for specific uses	19
A forward-thinking biocompatibility assessment: drafting future considerations now	19
Holistic ecosystem strategies for boosting biopharma start-up success	19
Establishment of a nonhomologous end joining-mediated genomic library in Fusarium fujikuroi for high-level production of gibberellic acid	19
Efficiency and process development for microbial biomass production using oxic bioelectrosynthesis	18
Engineering next-generation oxygen-generating scaffolds to enhance bone regeneration	18
CarveAdornCurate: a versatile cloud-based platform for constructing multiscale metabolic models	18
Biosensors for healthcare: current and future perspectives	18
Driving towards digital biomanufacturing by CHO genome-scale models	18