Trends in Biotechnology

Article	Citations
Plant Microbiome Engineering: Expected Benefits for Improved Crop Growth and Resilience	250
Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication	202
Microbiome Engineering: Synthetic Biology of Plant-Associated Microbiomes in Sustainable Agriculture	200
Cold Plasma, a New Hope in the Field of Virus Inactivation	177
Enhancing Biopolymer Hydrogel Functionality through Interpenetrating Networks	176
Grand Challenges for Industrializing Polyhydroxyalkanoates (PHAs)	176
Uncovering an Organ’s Molecular Architecture at Single-Cell Resolution by Spatially Resolved Transcriptomics	165
Enzyme discovery and engineering for sustainable plastic recycling	164
Reap the crop wild relatives for breeding future crops	158
On-Site Therapeutic Drug Monitoring	151
Rolling Circle Replication for Biosensing, Bioimaging, and Biomedicine	147
In situ Biogas Upgrading by CO2-to-CH4 Bioconversion	130
Microbial Electrosynthesis: Where Do We Go from Here?	127
Textile Triboelectric Nanogenerators for Wearable Pulse Wave Monitoring	116
Emergence of the Stem Cell Secretome in Regenerative Engineering	114
From Acetate to Bio-Based Products: Underexploited Potential for Industrial Biotechnology	111
Advances in the Fabrication of Biomaterials for Gradient Tissue Engineering	111
Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials	107
Non-Hydrolyzable Plastics – An Interdisciplinary Look at Plastic Bio-Oxidation	104
New Twists in Detecting mRNA Modification Dynamics	102
Decellularized Extracellular Matrix for Bioengineering Physiomimetic 3D in Vitro Tumor Models	101
Gallium-Based Liquid Metal Particles for Therapeutics	95
Prospective Therapeutic Applications of Platelet Extracellular Vesicles	92
Animal Immunization, in Vitro Display Technologies, and Machine Learning for Antibody Discovery	90
Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2	86

Material–Microbe Interfaces for Solar-Driven CO2 Bioelectrosynthesis	82
THz medical imaging: from in vitro to in vivo	77
Multitechnology Biofabrication: A New Approach for the Manufacturing of Functional Tissue Structures?	74
Biotechnological Exploration of Transformed Root Culture for Value-Added Products	73
Creative biological lignin conversion routes toward lignin valorization	72
Hypes, hopes, and the way forward for microalgal biotechnology	70
The tremendous biomedical potential of bacterial extracellular vesicles	69
Enzyme Catalyst Engineering toward the Integration of Biocatalysis and Chemocatalysis	68
Engineered Living Materials: Taxonomies and Emerging Trends	67
Microalgae-based biotechnological sequestration of carbon dioxide for net zero emissions	66
Modeling the Human Body on Microfluidic Chips	64
Flexible Fungal Materials: Shaping the Future	64
Electron Storage in Electroactive Biofilms	64
Using Microbial Aggregates to Entrap Aqueous Phosphorus	63
Engineered Methanotrophy: A Sustainable Solution for Methane-Based Industrial Biomanufacturing	63
Intrinsically Conductive Microbial Nanowires for ‘Green’ Electronics with Novel Functions	62
What makes Yarrowia lipolytica well suited for industry?	61
The Sustainable Path to a Circular Bioeconomy	61
Graphene for Biosensing Applications in Point-of-Care Testing	61
Combining Environmental and Economic Performance for Bioprocess Optimization	60
Sustainable Seaweed Biotechnology Solutions for Carbon Capture, Composition, and Deconstruction	59
In Situ 3D Printing: Opportunities with Silk Inks	58
Can Bottom-Up Synthetic Biology Generate Advanced Drug-Delivery Systems?	57
CRISPR/Cas-Based Epigenome Editing: Advances, Applications, and Clinical Utility	57
Enhanced Strategies for Antibiotic Removal from Swine Wastewater in Anaerobic Digestion	56
Prime editing: advances and therapeutic applications	56
P450-driven plastic-degrading synthetic bacteria	55
Host Systems for the Production of Recombinant Spider Silk	55
Oxygen-Releasing Biomaterials: Current Challenges and Future Applications	55
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution	53
Next-Generation CRISPR Technologies and Their Applications in Gene and Cell Therapy	52
Biosensors for healthcare: current and future perspectives	52
Quality by Design for enabling RNA platform production processes	50
Innovation trends in industrial biotechnology	50
Designing Artificial Cells towards a New Generation of Biosensors	49
Bioprocessing technology of muscle stem cells: implications for cultured meat	49
Recent Advances in the Microbial Synthesis of Hemoglobin	49
Putting Photosystem I to Work: Truly Green Energy	47
Synthetic Biology Approaches To Enhance Microalgal Productivity	47
Single-cell mass spectrometry	47
Synthetic Biological Circuits within an Orthogonal Central Dogma	46
Bioengineering artificial blood vessels from natural materials	46
Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering	46
Food Waste: A Promising Source of Sustainable Biohydrogen Fuel	45
Progress toward a bicarbonate-based microalgae production system	44
In situ bioprinting: intraoperative implementation of regenerative medicine	44
Nano toolbox in immune modulation and nanovaccines	43
Degradation or humification: rethinking strategies to attenuate organic pollutants	43
Microphysiological Systems to Recapitulate the Gut–Kidney Axis	43
Approaches for bacteriophage genome engineering	43

Innovative Tools and Strategies for Optimizing Yeast Cell Factories	43
Quantification of tumor heterogeneity: from data acquisition to metric generation	43
Production of Bio-alkanes from Biomass and CO2	43
Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges	42
Image-Based Live Cell Sorting	42
Emerging Argonaute-based nucleic acid biosensors	42
Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing	42
Aptamers targeting SARS-COV-2: a promising tool to fight against COVID-19	42
Toward Deep Biophysical Cytometry: Prospects and Challenges	42
Harnessing the Power of Enzymes for Tailoring and Valorizing Lignin	41
Microbial lignin valorization through depolymerization to aromatics conversion	41
Pushing the rheological and mechanical boundaries of extrusion-based 3D bioprinting	41
Using Co-Culture to Functionalize Clostridium Fermentation	40
Does RNAi-Based Technology Fit within EU Sustainability Goals?	40
The genome editing revolution	40
Phytohormone-like small biomolecules for microalgal biotechnology	40
Biocatalysis in Green and Blue: Cyanobacteria	39
3D-bioprinted cancer-on-a-chip: level-up organotypic in vitro models	39
Machine learning in bioprocess development: from promise to practice	39
SWATH-MS-Based Proteomics: Strategies and Applications in Plants	38
Smart materials: rational design in biosystems via artificial intelligence	38
Exploiting unconventional prokaryotic hosts for industrial biotechnology	38
Engineering Metabolism in Nicotiana Species: A Promising Future	38
Artificial intelligence and machine learning applications in biopharmaceutical manufacturing	38
CRISPR-Cas-mediated diagnostics	38
Renewable plant-derived lignin for electrochemical energy systems	37
Reverse Transcriptase: From Transcriptomics to Genome Editing	36
Genetic engineering of the acidophilic chemolithoautotroph Acidithiobacillus ferrooxidans	36
Microbiome-Inspired Green Infrastructure: A Toolkit for Multidisciplinary Landscape Design	36
Synthetic biology of extremophiles: a new wave of biomanufacturing	36
Fabricating Organized Elastin in Vascular Grafts	36
Multiproduct biorefinery from marine thraustochytrids towards a circular bioeconomy	35
Bioinspired materials and technology for advanced cryopreservation	35
Magnetic Levitation Systems for Disease Diagnostics	34
Discovery and delivery strategies for engineered live biotherapeutic products	33
Allergenicity Assessment of Novel Food Proteins: What Should Be Improved?	33
Microbial protein cell factories fight back?	33
Self-Sufficient Class VII Cytochromes P450: From Full-Length Structure to Synthetic Biology Applications	33
Biomimetic 3D living materials powered by microorganisms	33
Synthetic Methylotrophy in Yeasts: Towards a Circular Bioeconomy	33
Including Digital Sequence Data in the Nagoya Protocol Can Promote Data Sharing	32
Centimeter-Long Microbial Electron Transport for Bioremediation Applications	32
From Sequencing to Genome Editing for Cotton Improvement	32
Comparison of CRISPR/Cas and Argonaute for nucleic acid tests	32
Biowaste upcycling into second-generation microbial protein through mixed-culture fermentation	31
Advances and challenges in photosynthetic hydrogen production	31
Managing the Invasive Fall Armyworm through Biotech Crops: A Chinese Perspective	31
Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches	31
The Importance of Computational Modeling in Stem Cell Research	30
Micro-aeration: an attractive strategy to facilitate anaerobic digestion	30
Carbon-negative synthetic biology: challenges and emerging trends of cyanobacterial technology	30
Organs-on-a-chip: a union of tissue engineering and microfabrication	30
Induced pluripotent stem cell-derived engineered T cells, natural killer cells, macrophages, and dendritic cells in immunotherapy	29
Fast biofoundries: coping with the challenges of biomanufacturing	29
Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes	29
Robustness: linking strain design to viable bioprocesses	29
Microvascular Fragments: More Than Just Natural Vascularization Units	29
Linker Engineering in the Context of Synthetic Protein Switches and Sensors	29
Haloarchaea, excellent candidates for removing pollutants from hypersaline wastewater	28
Microfluidics in Single-Cell Virology: Technologies and Applications	28
Discovery and mining of enzymes from the human gut microbiome	27
Evading and overcoming AAV neutralization in gene therapy	27
Creating Bioethics Distance Learning Through Virtual Reality	27
Synthetic biology: a new frontier in food production	27
Biorefining waste into nanobiotechnologies can revolutionize sustainable agriculture	27
Carotenoid Biofortification of Crops in the CRISPR Era	26
Plant synthetic biology innovations for biofuels and bioproducts	26
Build a Sustainable Vaccines Industry with Synthetic Biology	26
Novel Modalities in DNA Data Storage	26
Progress and challenges in mass spectrometry-based analysis of antibody repertoires	26
From Innovation to Application: Bridging the Valley of Death in Industrial Biotechnology	26
Metabolic engineering for sustainability and health	25
What can biofabrication do for space and what can space do for biofabrication?	25
Synthetic Biology towards Engineering Microbial Lignin Biotransformation	25
Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes	25
Adaptive Evolution Improves Algal Strains for Environmental Remediation	25
CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics	24
In Vitro Models of Intestine Innate Immunity	24
Killing two birds with one stone: chemical and biological upcycling of polyethylene terephthalate plastics into food	24
Strategies for optimizing acetyl-CoA formation from glucose in bacteria	24

Gene Delivery to the Skin – How Far Have We Come?	24
Microbial genetic engineering approach to replace shark livering for squalene	24
SynMADE: synthetic microbiota across diverse ecosystems	24
Synovial joint-on-a-chip for modeling arthritis: progress, pitfalls, and potential	23
Reductive Glycine Pathway: A Versatile Route for One-Carbon Biotech	23
Microfluidics: the propellant of CRISPR-based nucleic acid detection	23
RNA-Cleaving NAzymes: The Next Big Thing in Biosensing?	23
Dynamic regulation of gut Clostridium-derived short-chain fatty acids	23
Are integrated bioelectrochemical technologies feasible for wastewater management?	23
Magnetic levitation for space exploration	22
RNA-Centric Methods: Toward the Interactome of Specific RNA Transcripts	22
Multiscale models quantifying yeast physiology: towards a whole-cell model	22
Tuning chitosan’s chemical structure for enhanced biological functions	22
Prospects for carbon-negative biomanufacturing	22
Proximity labeling: an enzymatic tool for spatial biology	22
Multiproduct Microalgae Biorefineries Mediated by Ionic Liquids	22
Volatile Siloxanes Emissions: Impact and Sustainable Abatement Perspectives	22
A pathway to improve seaweed aquaculture through microbiota manipulation	21
Substrate-independent and widely applicable deposition of antibacterial coatings	21
Towards in silico Process Modeling for Vaccines	21
Agricultural biotechnology for sustainable food security	21
Rise of the RNA machines – self-amplification in mRNA vaccine design	21
Biotechnology of functional proteins and peptides for hair cosmetic formulations	20
Extracellular Vesicles in Cardiac Regeneration: Potential Applications for Tissues-on-a-Chip	20
Roadmap to affinity-tuned antibodies for enhanced chimeric antigen receptor T cell function and selectivity	20
Regenerating dynamic organs using biomimetic patches	20
Functionalization of Cellular Membranes with DNA Nanotechnology	20
Molecular farming using transgenic rice endosperm	20
Vibrio natriegens as a host for rapid biotechnology	20
Alternative fat: redefining adipocytes for biomanufacturing cultivated meat	19
Customizing lipids from oleaginous microbes: leveraging exogenous and endogenous approaches	19
Thraustochytrid Cell Factories for Producing Lipid Compounds	19
Public acceptance and stakeholder views of gene edited foods: a global overview	19
The simpler, the better: tissue vascularization using the body’s own resources	19
Game-changing alternatives to conventional fungicides: small RNAs and short peptides	19
Iron oxide nanoparticle-based nanocomposites in biomedical application	19
Litter to Leaf: The Unexplored Potential of Silk Byproducts	19
Nanocell hybrids for green chemistry	19
Emerging immunomodulatory strategies for cell therapeutics	19
Factors affecting the competitiveness of bacterial fermentation	19
Single-cell analysis of circulating tumour cells: enabling technologies and clinical applications	19