Trends in Biotechnology

Article	Citations
Technologies and Standardization in Research on Extracellular Vesicles	235
Metabolic Engineering of Escherichia coli for Natural Product Biosynthesis	212
Plant Microbiome Engineering: Expected Benefits for Improved Crop Growth and Resilience	209
3D Printing in Suspension Baths: Keeping the Promises of Bioprinting Afloat	176
Microbiome Engineering: Synthetic Biology of Plant-Associated Microbiomes in Sustainable Agriculture	160
Cold Plasma, a New Hope in the Field of Virus Inactivation	158
High-Throughput Screening Technology in Industrial Biotechnology	158
Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication	157
Molecularly Imprinted Polymers for Cell Recognition	154
Enabling Technologies for Personalized and Precision Medicine	152
Integrative Methods and Practical Challenges for Single-Cell Multi-omics	146
Future Applications of MXenes in Biotechnology, Nanomedicine, and Sensors	141
Grand Challenges for Industrializing Polyhydroxyalkanoates (PHAs)	138
Uncovering an Organ’s Molecular Architecture at Single-Cell Resolution by Spatially Resolved Transcriptomics	138
Injectable Cryogels for Biomedical Applications	133
Enhancing Biopolymer Hydrogel Functionality through Interpenetrating Networks	131
Rolling Circle Replication for Biosensing, Bioimaging, and Biomedicine	126
On-Site Therapeutic Drug Monitoring	124
Phaeodactylum tricornutum: A Diatom Cell Factory	121
Enzyme discovery and engineering for sustainable plastic recycling	118
Reap the crop wild relatives for breeding future crops	112
In situ Biogas Upgrading by CO2-to-CH4 Bioconversion	109
Protein Engineering for Improving and Diversifying Natural Product Biosynthesis	109
The Business of Cultured Meat	102
Microbial Electrosynthesis: Where Do We Go from Here?	99

Textile Triboelectric Nanogenerators for Wearable Pulse Wave Monitoring	96
Ultrafast Photonic PCR Based on Photothermal Nanomaterials	96
From Acetate to Bio-Based Products: Underexploited Potential for Industrial Biotechnology	93
Advances in the Fabrication of Biomaterials for Gradient Tissue Engineering	92
Synthetic Biochemistry: The Bio-inspired Cell-Free Approach to Commodity Chemical Production	91
New Twists in Detecting mRNA Modification Dynamics	88
Non-Hydrolyzable Plastics – An Interdisciplinary Look at Plastic Bio-Oxidation	87
Emergence of the Stem Cell Secretome in Regenerative Engineering	85
Gallium-Based Liquid Metal Particles for Therapeutics	84
Extracellular Matrix Mimics Using Hyaluronan-Based Biomaterials	83
Intravesical Hydrogels as Drug Reservoirs	82
Burkholderia: An Untapped but Promising Bacterial Genus for the Conversion of Aromatic Compounds	82
Natural Medicinal Compounds in Bone Tissue Engineering	82
Decellularized Extracellular Matrix for Bioengineering Physiomimetic 3D in Vitro Tumor Models	80
Microbial Chassis Development for Natural Product Biosynthesis	79
Genetic Biosensor Design for Natural Product Biosynthesis in Microorganisms	77
Synthetic Biology and Genome-Editing Tools for Improving PHA Metabolic Engineering	76
Prospective Therapeutic Applications of Platelet Extracellular Vesicles	76
Leveling Up Hydrogels: Hybrid Systems in Tissue Engineering	72
Metabolic Engineering of Cupriavidus necator H16 for Sustainable Biofuels from CO2	72
Animal Immunization, in Vitro Display Technologies, and Machine Learning for Antibody Discovery	72
Bacterial Inclusion Bodies: A Treasure Trove of Bioactive Proteins	72
Controlling Voltage Reversal in Microbial Fuel Cells	70
Material–Microbe Interfaces for Solar-Driven CO2 Bioelectrosynthesis	68
Biotechnological Exploration of Transformed Root Culture for Value-Added Products	67
Multitechnology Biofabrication: A New Approach for the Manufacturing of Functional Tissue Structures?	64
Toward an Optimized Process for Clinical Manufacturing of CAR-Treg Cell Therapy	64
Elastin Biomaterials in Dermal Repair	63
Towards a Digital Bioprocess Replica: Computational Approaches in Biopharmaceutical Development and Manufacturing	62
Intraoperative Bioprinting: Repairing Tissues and Organs in a Surgical Setting	61
Bioengineered 3D Models to Recapitulate Tissue Fibrosis	58
Enzyme Catalyst Engineering toward the Integration of Biocatalysis and Chemocatalysis	58
Using Remote Fields for Complex Tissue Engineering	58
Developing a Computational Framework To Advance Bioprocess Scale-Up	55
Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing	55
Engineered Living Materials: Taxonomies and Emerging Trends	55
Electron Storage in Electroactive Biofilms	55
Using Microbial Aggregates to Entrap Aqueous Phosphorus	54
Microalgal Torrefaction for Solid Biofuel Production	54
THz medical imaging: from in vitro to in vivo	54
Intrinsically Conductive Microbial Nanowires for ‘Green’ Electronics with Novel Functions	54
Process Analytical Technologies and Data Analytics for the Manufacture of Monoclonal Antibodies	52
Graphene for Biosensing Applications in Point-of-Care Testing	52
Modeling the Human Body on Microfluidic Chips	51
Can Bottom-Up Synthetic Biology Generate Advanced Drug-Delivery Systems?	50
Combining Environmental and Economic Performance for Bioprocess Optimization	50
The Sustainable Path to a Circular Bioeconomy	50
Next-Generation CRISPR Technologies and Their Applications in Gene and Cell Therapy	50
Obscure yet Promising Oleaginous Yeasts for Fuel and Chemical Production	50
Engineered Methanotrophy: A Sustainable Solution for Methane-Based Industrial Biomanufacturing	50

Gelatin Matrices for Growth Factor Sequestration	50
Enhanced Strategies for Antibiotic Removal from Swine Wastewater in Anaerobic Digestion	49
Flexible Fungal Materials: Shaping the Future	48
Sustainable Seaweed Biotechnology Solutions for Carbon Capture, Composition, and Deconstruction	48
In Situ 3D Printing: Opportunities with Silk Inks	48
Designing Synthetic Microbial Consortia for Biofuel Production	46
Microdroplet-Assisted Screening of Biomolecule Production for Metabolic Engineering Applications	45
Novel Strategies and Platforms for Industrial Isoprenoid Engineering	45
Microalgae-based biotechnological sequestration of carbon dioxide for net zero emissions	45
Oxygen-Releasing Biomaterials: Current Challenges and Future Applications	44
Biotechnological Advances in the Design of Algae-Based Biosensors	44
Options to Reform the European Union Legislation on GMOs: Scope and Definitions	43
Engineering Microphysiological Immune System Responses on Chips	43
Microbial Coculture for Flavonoid Synthesis	42
CRISPR/Cas-Based Epigenome Editing: Advances, Applications, and Clinical Utility	42
Plant Aromatic Prenyltransferases: Tools for Microbial Cell Factories	42
Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering	42
Host Systems for the Production of Recombinant Spider Silk	42
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution	42
Putting Photosystem I to Work: Truly Green Energy	41
Synthetic Biology Approaches To Enhance Microalgal Productivity	40
Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals	40
Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways	39
Synthetic Biological Circuits within an Orthogonal Central Dogma	39
Creative biological lignin conversion routes toward lignin valorization	39
Cancer Immunoimaging with Smart Nanoparticles	38
Toward Deep Biophysical Cytometry: Prospects and Challenges	38
What makes Yarrowia lipolytica well suited for industry?	37
The tremendous biomedical potential of bacterial extracellular vesicles	37
SWATH-MS-Based Proteomics: Strategies and Applications in Plants	37
Bioprocessing technology of muscle stem cells: implications for cultured meat	36
Does RNAi-Based Technology Fit within EU Sustainability Goals?	36
Production of Bio-alkanes from Biomass and CO2	36
Image-Based Live Cell Sorting	36
P450-driven plastic-degrading synthetic bacteria	35
Progress toward a bicarbonate-based microalgae production system	35
Innovative Tools and Strategies for Optimizing Yeast Cell Factories	35
Single-cell mass spectrometry	35
Contemporary Tools for Regulating Gene Expression in Bacteria	35
Microbial Lipid Biotechnology to Produce Polyunsaturated Fatty Acids	35
Recent Advances in the Microbial Synthesis of Hemoglobin	35
Gut Check Time: Antibiotic Delivery Strategies to Reduce Antimicrobial Resistance	34
Designing Artificial Cells towards a New Generation of Biosensors	34
Bioengineering artificial blood vessels from natural materials	34
Harnessing the Power of Enzymes for Tailoring and Valorizing Lignin	34
Hypes, hopes, and the way forward for microalgal biotechnology	34
Next-Generation Sequencing: An Eye-Opener for the Surveillance of Antiviral Resistance in Influenza	34
Waste or Gold? Bioelectrochemical Resource Recovery in Source-Separated Urine	34
Quality by Design for enabling RNA platform production processes	33
Pushing the rheological and mechanical boundaries of extrusion-based 3D bioprinting	33
3D-bioprinted cancer-on-a-chip: level-up organotypic in vitro models	33
Microbiome-Inspired Green Infrastructure: A Toolkit for Multidisciplinary Landscape Design	32
Exploiting unconventional prokaryotic hosts for industrial biotechnology	32
Food Waste: A Promising Source of Sustainable Biohydrogen Fuel	32
Microphysiological Systems to Recapitulate the Gut–Kidney Axis	32
Engineering Metabolism in Nicotiana Species: A Promising Future	32
Biocatalysis in Green and Blue: Cyanobacteria	31
Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing	31
Nano toolbox in immune modulation and nanovaccines	30
Multiproduct biorefinery from marine thraustochytrids towards a circular bioeconomy	30
Emerging Argonaute-based nucleic acid biosensors	29
Fifty Shades of Blue: How Blue Biotechnology is Shaping the Bioeconomy	29
Reverse Transcriptase: From Transcriptomics to Genome Editing	29
Magnetic Levitation Systems for Disease Diagnostics	29
Synthetic Methylotrophy in Yeasts: Towards a Circular Bioeconomy	29
Including Digital Sequence Data in the Nagoya Protocol Can Promote Data Sharing	29
Quantification of tumor heterogeneity: from data acquisition to metric generation	29
Aptamers targeting SARS-COV-2: a promising tool to fight against COVID-19	28
The Formidable Challenge of Controlling High Mannose-Type N-Glycans in Therapeutic mAbs	28
Fabricating Organized Elastin in Vascular Grafts	28
Geneva Statement on Heritable Human Genome Editing: The Need for Course Correction	28
Using Co-Culture to Functionalize Clostridium Fermentation	28
CRISPR-Based Directed Evolution for Crop Improvement	28
Allergenicity Assessment of Novel Food Proteins: What Should Be Improved?	28
Advancing Mass Spectrometry Technology in cGMP Environments	28
100 Years Later, What Is New in Glycerol Bioproduction?	28
Biomimetic 3D living materials powered by microorganisms	27
Innovation trends in industrial biotechnology	27
Degradation or humification: rethinking strategies to attenuate organic pollutants	27
Managing the Invasive Fall Armyworm through Biotech Crops: A Chinese Perspective	27

In situ bioprinting: intraoperative implementation of regenerative medicine	27
Self-Sufficient Class VII Cytochromes P450: From Full-Length Structure to Synthetic Biology Applications	27
Bioinspired materials and technology for advanced cryopreservation	26
Smart materials: rational design in biosystems via artificial intelligence	26
Microbial lignin valorization through depolymerization to aromatics conversion	26
Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges	26
Genetic engineering of the acidophilic chemolithoautotroph Acidithiobacillus ferrooxidans	26
Microvascular Fragments: More Than Just Natural Vascularization Units	25
Microbial protein cell factories fight back?	25
Synthetic Protein Scaffolding at Biological Membranes	25
Decellularized Scaffolds for Intervertebral Disc Regeneration	25
Phytohormone-like small biomolecules for microalgal biotechnology	25
Linker Engineering in the Context of Synthetic Protein Switches and Sensors	25
From Sequencing to Genome Editing for Cotton Improvement	24
Centimeter-Long Microbial Electron Transport for Bioremediation Applications	24
Haloarchaea, excellent candidates for removing pollutants from hypersaline wastewater	24
Build a Sustainable Vaccines Industry with Synthetic Biology	24
Microfluidics in Single-Cell Virology: Technologies and Applications	24
The Importance of Computational Modeling in Stem Cell Research	24
Robustness: linking strain design to viable bioprocesses	23
Adaptive Evolution Improves Algal Strains for Environmental Remediation	23
Prime editing: advances and therapeutic applications	23
Regulation of Metabolic Homeostasis in Cell Culture Bioprocesses	23
Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes	23
Creating Bioethics Distance Learning Through Virtual Reality	22
Machine learning in bioprocess development: from promise to practice	22
Discovery and delivery strategies for engineered live biotherapeutic products	22
Biosensors for healthcare: current and future perspectives	22
Advances and challenges in photosynthetic hydrogen production	22
Approaches for bacteriophage genome engineering	22
Functional Applications of Nucleic Acid–Protein Hybrid Nanostructures	22
CRISPR-Cas-mediated diagnostics	22
Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes	22
Progress and challenges in mass spectrometry-based analysis of antibody repertoires	22
Gene Delivery to the Skin – How Far Have We Come?	22
The Pathway Less Traveled: Engineering Biosynthesis of Nonstandard Functional Groups	21
Comparison of CRISPR/Cas and Argonaute for nucleic acid tests	21
Discovery and mining of enzymes from the human gut microbiome	21
Minicircle DNA: The Future for DNA-Based Vectors?	21
From Innovation to Application: Bridging the Valley of Death in Industrial Biotechnology	21
What can biofabrication do for space and what can space do for biofabrication?	21
Novel Modalities in DNA Data Storage	20
Thraustochytrid Cell Factories for Producing Lipid Compounds	20
Carotenoid Biofortification of Crops in the CRISPR Era	20
Synthetic Biology towards Engineering Microbial Lignin Biotransformation	20
Reductive Glycine Pathway: A Versatile Route for One-Carbon Biotech	20
RNA-Cleaving NAzymes: The Next Big Thing in Biosensing?	20
Renewable plant-derived lignin for electrochemical energy systems	20
Bioprocess Technologies that Preserve the Quality of iPSCs	20
Fast biofoundries: coping with the challenges of biomanufacturing	20
RNA-Centric Methods: Toward the Interactome of Specific RNA Transcripts	19
Multiproduct Microalgae Biorefineries Mediated by Ionic Liquids	19
SynMADE: synthetic microbiota across diverse ecosystems	19
Bioelectrofuel Synthesis by Nanoenzymes: Novel Alternatives to Conventional Enzymes	19
Microbial genetic engineering approach to replace shark livering for squalene	18
Functionalization of Cellular Membranes with DNA Nanotechnology	18
In Vitro Models of Intestine Innate Immunity	18
The genome editing revolution	18
Strategies for optimizing acetyl-CoA formation from glucose in bacteria	18
Biorefining waste into nanobiotechnologies can revolutionize sustainable agriculture	17
Synthetic biology: a new frontier in food production	17
Towards in silico Process Modeling for Vaccines	17
Plant synthetic biology innovations for biofuels and bioproducts	17
Volatile Siloxanes Emissions: Impact and Sustainable Abatement Perspectives	17
Vibrio natriegens as a host for rapid biotechnology	17
Leveraging Single-Cell Sequencing for Chimeric Antigen Receptor T Cell Therapies	17
Magnetic levitation for space exploration	17
Killing two birds with one stone: chemical and biological upcycling of polyethylene terephthalate plastics into food	17
Multiscale models quantifying yeast physiology: towards a whole-cell model	17
Engineering Biocatalytic Solar Fuel Production: The PHOTOFUEL Consortium	17
Metabolic engineering for sustainability and health	17
Synthetic biology of extremophiles: a new wave of biomanufacturing	17
CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics	16
Improving aptamer performance with nucleic acid mimics: de novo and post-SELEX approaches	16
Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?	16
Biowaste upcycling into second-generation microbial protein through mixed-culture fermentation	16
Roadmap to affinity-tuned antibodies for enhanced chimeric antigen receptor T cell function and selectivity	16
Harnessing SLE Autoantibodies for Intracellular Delivery of Biologic Therapeutics	16
Extracellular Vesicles in Cardiac Regeneration: Potential Applications for Tissues-on-a-Chip	16
Carbon-negative synthetic biology: challenges and emerging trends of cyanobacterial technology	16
Deciphering Organoids: High-Dimensional Analysis of Biomimetic Cultures	15
New Cognitive Neurotechnology Facilitates Studies of Cortical–Subcortical Interactions	15
A Shift Towards Biotechnology: Social Opinion in the EU	15
Artificial intelligence and machine learning applications in biopharmaceutical manufacturing	15
Prospects for carbon-negative biomanufacturing	15
Intelligent Platelet Morphometry	15
Regenerating dynamic organs using biomimetic patches	15
Cellular reprogramming and the rise of rejuvenation biotech	15
Genetic Safeguards for Safety-by-design: So Close Yet So Far	15
Organs-on-a-chip: a union of tissue engineering and microfabrication	14
Smart Nucleic Acids as Future Therapeutics	14
Nanocell hybrids for green chemistry	14
Options to Reform the European Union Legislation on GMOs: Risk Governance	14
The Biofilms Structural Database	14
Evading and overcoming AAV neutralization in gene therapy	14