Trends in Biotechnology

Article	Citations
Subscription and Copyright information	190
Engineering biology approaches to modulate bacterial biofilms	181
Advisory Board and Contents	163
Unlocking the potential of stem-cell-derived ‘synthetic’ embryo models	161
Smart materials: rational design in biosystems via artificial intelligence	159
Phytohormones: novel strategy for removing emerging contaminants and recovering resources	158
The green revolution of food waste upcycling to produce polyhydroxyalkanoates	154
Electronic plants: the future of agriculture and urban ecosystems?	126
Scalable tissue biofabrication via perfusable hollow fiber arrays for cultured meat applications	125
Manipulating immune activity of macrophages: a materials and mechanics perspective	124
Bridging the path to multiplexed molecular diagnostics	123
Auricular repair: symphony between scaffolds and stem cells	116
Vector-free intra-airway in vivo epigenetic editing	112
Healing wounds with plasma-activated hydrogel therapy	111
Cofactor engineering powers next-generation biomanufacturing	111
Prime editing: advances and therapeutic applications	100
SynMADE: synthetic microbiota across diverse ecosystems	98
Microalgae: a revolution for salt-affected soil remediation	94
Analyzing and understanding the robustness of bioprocesses	92
Advisory Board and Contents	86
Subscription and Copyright information	82
Subscription and Copyright information	81
Subscription and Copyright information	81
Next-generation digital biomarkers: continuous molecular health monitoring using wearable devices	81
UFSC Biotech Network: a transdisciplinary platform for biotechnology innovation in Brazil	80

Influence of titer, rate, yield, and scale on the cost and life-cycle emissions of biomanufacturing	78
Molecular design of microalgae as sustainable cell factories	76
Engineering the mangrove soil microbiome for selection of polyethylene terephthalate-transforming bacterial consortia	76
Manufacturing innovation to drive down cell therapy costs	76
Emerging strategies to develop novel genetically encoded biosensors	75
Advisory Board and Contents	73
The GPS for drug development: navigating with evidence from human populations	73
Ocular RNA nanomedicine: engineered delivery nanoplatforms in treating eye diseases	72
Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis	72
Towards biomanufacturing of cultured meat	72
Extended human lymph node explants for evaluation of adaptive immunity	71
Translational organoid technology – the convergence of chemical, mechanical, and computational biology	71
Printhead on a chip: empowering droplet-based bioprinting with microfluidics	70
Mini-bones: miniaturized bone in vitro models	70
A high-performance protein preparation approach in a single column-free step	69
Integrating bicarbonate-based microalgal production with alkaline sewage for ocean negative carbon emissions	69
Can protein expression be ‘solved’?	69
Machine learning to predict de novo protein–protein interactions	69
Beyond waste: understanding urine’s potential in precision medicine	67
Nanobotic microbiomes against emerging vascular pathogens in agriculture	67
Machine learning in bioprocess development: from promise to practice	66
Next-generation 3D-printed nutritious food derived from waste plastic and biomass	66
Advisory Board and Contents	66
The LEGO® brick road to open science and biotechnology	65
Subscription and Copyright information	65
Ethical challenges with 3D bioprinted tissues and organs	64
Advisory Board and Contents	64
Biosafety and biosecurity: treading China’s synbio tightrope	63
Dynamic plasmid copy number control for synthetic biology	63
3D extrusion bioprinting: rational bioink design and advanced fabrication techniques	62
Sebum analysis as an advantageous source of biomolecular information	61
Advisory Board and Contents	60
Advisory Board and Contents	59
Advances in lung biomimetic systems: exploring biophysical cues in lung regenerative medicine	58
Frog ‘N’ Roll: novel synthetic peptides selective against Gram-negative pathogens	57
Synthetic biology for plant genetic engineering and molecular farming	56
Emerging supramolecular and living materials in oral medicine	56
Harnessing Streptomyces–plant interactions for agricultural natural product discovery	54
Metabolic and immunomodulatory control of type 2 diabetes via generating cellular itaconate reservoirs by inflammatory-targeting gene-therapy nanovesicles	54
The morally disruptive future of reprogenetic enhancement technologies	54
Improving awareness, understanding, and enforcement of responsibilities and regulations in Blue Biotechnology	54
Nanobiomaterials to modulate natural killer cell responses for effective cancer immunotherapy	53
Subscription and Copyright information	53
Dynamically regulating metabolic fluxes with synthetic metabolons	52
3D printing of heart valves	51
Building biomaterials through genetic code expansion	51
A new chapter for anti-idiotypes in low molecular weight compound immunoassays	51
Estimating environmental impacts of early-stage bioprocesses	51
Bioprinted, spatially defined breast tumor microenvironment models of intratumoral heterogeneity and drug resistance	51
Selector adeno-associated viral vectors facilitate on-target precise genome editing and purge off-target chromosomal insertions	50

Small but mighty: emerging roles of uORFs in plants	50
Trends in sustainable single-cell protein from non-grain feedstocks	50
Engineered bacterial therapeutics with material solutions	50
Using synthetic biology to express nitrogenase biosynthesis pathway in rice and to overcome barriers of nitrogenase instability in plant cytosol	49
Spatial omics for accelerating plant research and crop improvement	49
Multienzymatic synthesis of nylon monomers from vegetable oils	49
Non-sterile cultivation of oleaginous organisms	47
Biotechnological approaches for producing natural pigments in yeasts	45
Advances in induced anhydrobiosis for cell and gamete storage	45
Design principles for engineering light-controlled antibodies	45
Rethinking techno-moral disruption in bioethics, society, and justice	44
Bringing the next biotechnologies to life	44
From academia to real-life biotech: ‘It takes a village’	44
Protein–glycan engineering in vaccine design: merging immune mechanisms with biotechnological innovation	44
Academic entrepreneurship: promise and perils	44
Molecular engineering of insulin for recombinant expression in yeast	44
Next-generation industrial biotechnology for low-cost mass production of PHA	43
Innovating biomanufacturing with coculture-based engineered living materials	43
One-pot production of colored bacterial cellulose	43
Rapid selection of transgenic mammalian cells via diphtheria toxin resistance	42
Breaking the immunosuppressive barrier: an armored CAR-M biotechnology with the original M1 phenotype dominance rescues antitumor immunity	42
Therapeutic microneedles for myocardial repair	41
Solar-driven PHB synthesis from wastewater by engineered semiconductor–bacteria biohybrids	41
Microbial electrosynthesis: opportunities for microbial pure cultures	41
Advanced strategies for enzyme–electrode interfacing in bioelectrocatalytic systems	40
Advisory Board and Contents	39
Subscription and Copyright information	38
Making cow-free caseins and casein micelles	38
Long-offset paired nicking-based efficient and precise strategy for in vivo targeted insertion	38
Robots with a human touch	38
Subscription and Copyright information	37
Nanobodies: a new frontier in plant disease management	37
How transdisciplinarity can help biotech-driven biodiversity research	37
Marine-derived bioactive materials as antibiofilm and antivirulence agents	37
Harnessing TLS polymerases to direct nucleotide outcomes in base editing	37
Advisory Board and Contents	36
CRISPR screen-based mammalian cell engineering for complex biotherapeutics	36
Computationally guided genome rewiring of Escherichia coli and its application for nanopolyethylene terephthalate (PET) biodegradation and upcycling	36
Bioprocessing technology of muscle stem cells: implications for cultured meat	36
Microfluidic-based skin-on-chip systems for safety assessment of nanomaterials	36
Epicardial patches for myocardial repair: circumventing barriers to heal ischemic heart tissue	35
HHV-6 and HHV-7 reactivation in allogeneic CAR-T cell therapy	34
Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration	34
Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes	34
Biosensor-guided indexing of genome evolution for boosting microbial chemical synthesis	34
CRISPR-Cas12c: a noncleaving DNA binder with minimal PAM requirement	33
Harnessing synthetic biology to develop novel psychedelic therapies	33
Virus-like particles as modular interfaces for biomaterial functionalization	33
Engineering M13 phages: surface and morphological strategies for programmable applications	33
Exploring standards for multicellular mammalian synthetic biology	32
Engineered RNA nanostructures for scalable and efficient RNAi-based pesticides	32
Efficient and sustained photosynthetic hydrogen production by algae under high light intensity	32
Transforming undergraduate laboratory courses with interlinked real-world challenges	32
Thermotolerant yeasts and their biotechnological applications	32
An epigenetic regulator synergizes with alphavirus-mediated gene therapy via biomimetic delivery for enhanced cancer therapy	32
Welfare impacts of China's regulatory change toward genome-edited crops	32
Product-driven high-throughput screening of industrial filamentous actinomycetes	31
CRISPR/Cas-based personal glucose meters for nucleic acid detection	31
Biotechnology applications of proteins functionalized with DNA oligonucleotides	31
The FDA’s plan to phase out animal testing	30
Clinical technology advances driving in vivo CAR therapy	30
Distillation for in situ recovery of volatile fermentation products	30
pHNRhCas9NG, single expression cassette-based dual-component dual-transcription unit CRISPR/Cas9 system for plant genome editing	30
Organ-on-a-chip systems for modeling tumor and normal tissue microenvironments in radiotherapy research	30
Light-induced programmable solid–liquid phase transition of biomolecular condensates for improved biosynthesis	30
Novel delivery systems for controlled release of bacterial therapeutics	30
RNA and nanocarriers: next generation drug and delivery platform take center stage	29
Advancing plant gene activation with CRISPR-Act3.0	29
Harnessing the microbial world for human benefit	29
Chips, guts, and gas: unraveling volatile microbial mysteries in real time!	29
Deep learning-guided discovery and engineering of binding peptides for accelerated enzymatic degradation of polyethylene terephthalate	28
Phase-separated biomolecular condensates for biocatalysis	28
Immunostimulation in experimental gas plasma therapy for breast cancer	28
OMEGA-guided DNA polymerases enable random mutagenesis in a tunable window	28
Synthetic M13 phage engagers expand CAR-T cell antigen recognition to overcome tumor heterogeneity	28
Rewiring holobiont systems with synthetic biology	27
Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation	27
Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production	27
Application of bacterial extracellular vesicles in gastrointestinal diseases	27
Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering	27

Prime editor integrase systems boost targeted DNA insertion and beyond	27
Harnessing piezoelectricity for musculoskeletal regeneration: microcurrents to tissue repair	26
Synthetic transcription factor engineering for cell and gene therapy	26
Gas plasma technology mediates deep tissue and anticancer events independently of hydrogen peroxide	26
Rational bioengineering of polysaccharide in designing of microbiome modulation	26
Ethical and legal considerations of digital animal models: pioneering reduction and replacement	26
Evading and overcoming AAV neutralization in gene therapy	25
Bringing carbon to life via one-carbon metabolism	25
Prokaryotic Argonautes for in vivo biotechnology and molecular diagnostics	25
Subscription and Copyright information	25
Human bone marrow organoids: emerging progress but persisting challenges	25
Dynamic anaerobic digestion-based biorefineries for on-demand renewable energy and bioproducts in a circular bioeconomy	25
Robustness: linking strain design to viable bioprocesses	25
Large language model for knowledge synthesis and AI-enhanced biomanufacturing	25
The biotechnology revolution in textile dyeing	24
Hydrogen production in microbial electrolysis cells with biocathodes	24
Programmable embedded bioprinting for one-step manufacturing of arterial models with customized contractile and metabolic functions	24
Advancing wheat breeding using rye: a key contribution to wheat breeding history	24
Intervertebral disc-on-a-chip: a precision engineered toolbox for low back pain studies	24
Next-generation electroactive biomaterials-integrated bioelectronics for advanced healthcare management	23
The lignocellulosic biorefinery concept is sound: a commentary on Zhao et al.	23
Bioinspired thermoreversible bioink orchestrates focal adhesion-dependent osteogenesis	23
Editing a healthier future in plants	23
Advisory Board and Contents	23
Yeast-based screening platforms to understand and improve human health	23
Advancements and challenges in CAR-NK cell therapy for cancer treatment	23
Artificial intelligence in biomaterials: a call for unified biocompatibility standards	23
Escherichia coli selection strains for growth-coupled metabolic engineering	23
Advancements and regulations of biomanufacturing cell-based cartilage repair therapies	23
Power-to-vitamins: producing folate (vitamin B9) from renewable electric power and CO2 with a microbial protein system	22
Bioart and biosafety: navigating ‘living art’ within the European regulatory framework	22
Subscription and Copyright information	22
Comparison of CRISPR/Cas and Argonaute for nucleic acid tests	22
Recent advances in systems engineering approaches to integrated strain and bioprocess development	22
Subscription and Copyright information	22
Framing synthetic biology as an innovation-driven market	22
Chimeric antigen receptor therapy meets mRNA technology	22
Shaping the future US bioeconomy through safety, security, sustainability, and social responsibility	22
Large-scale manufacturing of human gallbladder epithelial cell products and derived hepatocytes via a chemically defined approach	21
Pollutant degradation in saline wastewater using synthetic biology	21
Cadherin-engineered microspheres enable scalable biofabrication of multilineage liver organoids	21
Media representations of synthetic biology in China	21
Navigating microalgae under dynamic CO2 for waste-to-energy conversion	21
Engineering chimeric signaling proteins for microbial whole-cell biosensors: from design to deployment	21
One-pot diagnostic methods based on CRISPR/Cas and Argonaute nucleases: strategies and perspectives	21
Reimagining colour with synthetic biology	21
Synthetic biology for Taxol biosynthesis and sustainable production	21
Strengthening the competitiveness of EU in vitro biotechnologies	21
Companies: share cultivated meat breakthroughs publicly—here is how	21
Subscription and Copyright information	20
New technologies for production of recombinant spider silk	20
Insights on cisgenic plants with durable disease resistance under the European Green Deal	20
A forward-thinking biocompatibility assessment: drafting future considerations now	20
Cellular reprogramming and the rise of rejuvenation biotech	20
Towards machine learning for hydrogel drug delivery systems	20
Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapy	20
Osmotic-controlled release oral tablets: technology and functional insights	20
Subscription and Copyright information	20
Designing at-source and end-of-pipe biotechnologies to tackle pharmaceutical pollution	19
Engineering next-generation oxygen-generating scaffolds to enhance bone regeneration	19
Protein–nucleic acid thermodynamic databases for specific uses	19
Spatial -omics technologies: the new enterprise in 3D breast cancer models	19
Organs-on-chips: a decade of innovation	19
Holistic ecosystem strategies for boosting biopharma start-up success	19
Point-of-care detection devices for wound care and monitoring	19
Microbial cellulose production using ocean water: sustainable and cost-effective?	18
Establishment of a nonhomologous end joining-mediated genomic library in Fusarium fujikuroi for high-level production of gibberellic acid	18
Agricultural biotechnology for sustainable food security	18
Design of biomass-based renewable materials for environmental remediation	18
Driving towards digital biomanufacturing by CHO genome-scale models	18
Biosensors for healthcare: current and future perspectives	18
Advances in ultrahigh-throughput screening technologies for protein evolution	18
EccDNA in plant-stress and biotechnological solutions in agriculture	18
Innovation trends in industrial biotechnology	18
A pathway to improve seaweed aquaculture through microbiota manipulation	18
Plant-based models of neurodegenerative CAG repeat expansion diseases	18
Robotic micromotors transforming oral drug administration	18
Resilience-by-design in engineering Bacillus cell factories	18
Gene drive in species complexes: defining target organisms	18
A ventilated perfused lung model platform to dissect the response of the lungs to viral infection	18
TRYing to evaluate production costs in microbial biotechnology	18
Untethered: using remote magnetic fields for regenerative medicine	18
CarveAdornCurate: a versatile cloud-based platform for constructing multiscale metabolic models	17
Personalized, connected health enabled by AI and home-based diagnostics	17
Thermodynamic database supports deciphering protein–nucleic acid interactions	17
Engineering bacteria for production of sustainable polycyclopropanated jet fuel alternatives	17
Harnessing microbes to pioneer environmental biophotoelectrochemistry	17
Ethics and engagement: steering China’s synbio future	17
Subscription and Copyright information	17
Challenges in realizing therapeutic antibody biosensing	17
Subscription and Copyright information	17
Quality by Design for enabling RNA platform production processes	17
Advisory Board and Contents	17
Comment on ‘Thermodynamic database supports deciphering protein–nucleic acid interactions’	17
CRISPR-based systems for sensitive and rapid on-site COVID-19 diagnostics	17