Journal of Microbiological Methods

Article	Citations
Improved productivity and dye removal performance of Trametes versicolor pellets using rice husk as a co-substrate	36
Determination of bactericidal activity against 3HC-2-Tre-labelled Mycobacterium abscessus (Mycobacteroides abscessus) by automated fluorescence microscopy	35
Evaluation of VITEK® 2 and MALDI-TOF/MS automated methodologies in the identification of atypical Listeria spp. isolated from food in different regions of Brazil	34
Coupon position does not affect Pseudomonas aeruginosa and Staphylococcus aureus biofilm densities in the CDC biofilm reactor	30
Development of an immunochromatographic test for the detection of Mycoplasma pneumoniae GroES antigen	29
Objectification of evaluation criteria in microscopic agglutination test using deep learning	26
C6 peptide enzyme immunoassay in Lyme borreliosis serology	25
Comparison of Brucella abortus population structure based on genotyping methods with different levels of resolution	25
CtNorm: Real time PCR cycle of threshold (Ct) normalization algorithm	25
A novel PCR-based genotyping method for Proteus mirabilis – Intergenic region polymorphism analysis	23
Development of a novel visual isothermal amplification assay for rapid detection of Brucella spp.	22
Microcalorimetric growth behavior of E. coli ATCC 25922 in an MCDSC	21
Extracting the potential of advances in pathogen control and food diagnostics	21
A new killing assay with the Caenorhabditis elegans PX627 mutant to assess the virulence of uropathogenic Escherichia coli strains	21
Detection of Clostridioides difficile toxin B gene in clinical stool specimens using rapid diagnostic quenching probe-polymerase chain reaction assay	21
Novel mouse monoclonal antibodies against Bordetella pertussis pertactin antigen with versatile applications	21
Editorial Board	20
Improvement of the detection efficiency of 3M™ molecular detection system for Campylobacter in poultry using nitrogen-doped carbon nanodots	19
The spatial distribution of aerosols in high-speed bone burring with external irrigation	19
Comparative assessment of three RNA extraction methods for obtaining high-quality RNA from Candida viswanathii biomass	19
Evaluation of three molecular carbapenemase tests: Eazyplex SuperBug complete B, Novodiag CarbaR+, and Amplidiag CarbaR+MCR	18
Performance evaluation of a selective-enrichment-isolation protocol for Salmonella enterica from seafood	18
Editorial Board	18
Evaluation of a modified rapid viability-polymerase chain reaction method for Bacillus atrophaeus spores in water matrices	17
Development and optimization of a TaqMan assay for Nosema bombycis, causative agent of pébrine disease in Bombyx mori silkworm, based on the β-tubulin gene	17

Editorial Board	16
Method for screening antimicrobial gels against multi-species oral biofilms	16
Comparison of recombinant and synthetic listeriolysin- O peptide- based indirect ELISA vis-à-vis cultural isolation for detection of listeriosis in caprine and ovine species	15
Role of temperature, nutrition, oxygen, osmolality, and bacterial strain in inducing a viable but non-culturable state in Campylobacter jejuni	15
Evaluation of the simplified carbapenem inactivation method as a phenotypic detection method for carbapenemase-producing Enterobacterales	15
Development of a device for cultivation and isolation of microbes using a specialized cellulose film	15
A simple method for the cultivation of the “unculturable” asterinaceous fungi (Asterinales/Dothideomycetes)	15
Systematic review and critical reflection on the isolation and identification methods for spoilage associated bacteria in fresh marine fish	14
Towards digital diagnosis of malaria: How far have we reached?	14
Evaluation of phenotypic methods for detection of polymyxin B-resistant bacteria	14
Investigation of dense fine speckled pattern and anti-dense fine speckled 70 antibody by a single step assay	14
Measurement of lactose concentration in milk by using engineered bacteria producing lycopene	14
Mycoplasma hyopneumoniae detection by PCR in naturally infected finishing pigs	14
Construction of an experimental study and addition of adapter sequences using HiDi DNA polymerase for improving DNA normalization methods relevant to novel gene discovery	13
Cotton strip assay detects soil microbial degradation differences among crop rotation and tillage experiments on Vertisols	13
Short-length Homologous Region exhaustive Search algorithm (SHRS): A primer design algorithm for differentiating bacteria at the species, subspecies, or strain level based on a whole genome sequence	12
Quantitative detection of Ganodermati lucidum immunomodulatory protein-8 by a peptide-antigen-antibody sandwich ELISA	12
Development of a real-time quantitative PCR assay for detection and quantification of the marine bacterium Alteromonas macleodii from coastal environments	12
Engineering Citrobacter freundii using CRISPR/Cas9 system	12
Duplex real-time fluorescent recombinase polymerase amplification for the rapid and sensitive detection of two resistance genes in drug-resistant Staphylococcus aureus	12
Estimation of biosurfactant production parameters and yields without conducting additional experiments on a larger production scale	12
Use of recombinant malate dehydrogenase (MDH) and superoxide dismutase (SOD) [Cu Zn] as antigens in indirect ELISA for diagnosis of bovine brucellosis	11
Agrobacterium tumefaciens-mediated transformation of Nigrospora sp. isolated from switchgrass leaves and antagonistic toward plant pathogens	11
Assay of cellulose 1,4-β-cellobiosidase activity in soil	11
Editorial Board	11
Cell-free production of fluorescent proteins for the discovery of novel ribosome-targeting antibiotics	11
Evaluation of RapidChek® SELECT™ for the detection of Salmonella spp. in environmental samples from a veterinary hospital	11
Optimized bacterial absolute quantification method by qPCR using an exogenous bacterial culture as a normalization strategy in triple-species BV-like biofilms	11
Interrelationship among substrate utilization, metabolic productions, and housekeeping-related gene expression levels in Mortierella alpine CBS 754.68	10
Identification of emulsification proteins released from the cells by inhibiting the synthesis of GPI-anchor or β-1,3-glucan in Candida albicans	10
Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root	10
A method for high-throughput image-based antifungal screening	10
Development and comparative evaluation of droplet digital PCR and quantitative PCR for the detection and quantification of Chlamydia psittaci	10
Rapid cell counting and viability detection method of Escherichia coli Nissle using image cytometry	10
The performance of a Fully Automated Urine Particle Analyzer, Sysmex UF-5000, in detecting fastidious bacteria in urine samples	10
Development and evaluation of an agar capture system (ACS) for high-throughput screening of insoluble particulate substrates with bacterial growth and enzyme activity assays	10
Validation of a cariogenic biofilm model by evaluating the effect of fluoride on enamel demineralization	9
Fermentation dynamics of bile salt hydrolase production in Heyndrickxia coagulans ATCC 7050 and Lactiplantibacillus plantarum ATCC 10012: Addressing ninhydrin assay limitations with a novel HPTLC–MS m	9
FastFung: A novel medium for the culture and isolation of fastidious fungal species from clinical samples	9
Assessment of the capacity of Whatman filter papers as support to store stools for the molecular diagnostic testing of soil-transmitted helminthiasis	9
Campylobacter fetus subspecies specific PCR assays inferred from comparative genomic analysis for accurate subspecies identification	9
Comparative stability of assay results of enterococci measured by culture and qPCR over time in bathing beach waters	8
Detection of KPC directly from positive blood cultures by MALDI-TOF: From research to the clinical microbiology laboratory routine	8
Construction and optimization of a multiplex PMAxx-qPCR assay for viable Bacillus cereus and development of a detection kit	8
Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora	8
Development of real-time PCR methods for the quantification of Methanoculleus, Methanosarcina and Methanobacterium in anaerobic digestion	8
Comparative evaluation of analytical pipelines for illumina short- and nanopore long-read 16S rRNA gene amplicon sequencing with mock microbial communities	8
Classification of food spoilage bacterial species and their sodium chloride, sodium acetate and glycine tolerance using chemometrics analysis and Raman spectroscopy	8
A new technique to evaluate Acidithiobacillus thiooxidans growth during a bioleaching process based on DNA quantification	8
A new MALDI-TOF approach for the quick sequence type identification of Legionella pneumophila	8

Screening for acute disseminated histoplasmosis in HIV disease using urinary antigen detection enzyme immunoassay: A pilot study in Cameroon	8
Metagenomic next-generation sequencing of bronchoalveolar lavage fluid in non-severe and severe pneumonia patients	8
Rapid detection of plasmid-mediated AmpC-producers by eazyplex® SuperBug AmpC assay compared to whole-genome sequencing	8
Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART)	8
Combination of the electronic nose with microbiology as a tool for rapid detection of Salmonella	8
Research on the optimization, key chemical constituents and antibacterial activity of the essential oil extraction process of Thuja koraiensis Nakai	8
Assessment of stool collection and storage conditions for in vitro human gut model studies	8
Formalin-fixed paraffin-embedded tissues for microbiome analysis in rainbow trout (Oncorhynchus mykiss)	7
Identification and antibacterial activity of Thamnolia vermicularis and Thamnolia subuliformis	7
Automated antimicrobial susceptibility testing of slow-growing Pseudomonas aeruginosa strains in the presence of tetrazolium salt WST-1	7
Use of uncoated magnetic beads to capture Mycobacterium smegmatis and Mycobacterium avium paratuberculosis prior detection by mycobacteriophage D29 and real-time-PCR	7
A screening method for decoloration of xenobiotic dyes by fungi	7
Adaptation and validation of a light microscope for use in energy insecure settings: a proof-of-concept study	7
A strip-based assay for detection of CrfA enzyme activity to differentiate Mycobacterium tuberculosis and non-tuberculous mycobacteria	7
Viability of Saccharomyces cerevisiae during baking of bread dough by flow cytometry	7
Comparative performance of different antigens on the lateral flow assay (LFA) platform for the rapid serodiagnosis of paratuberculosis	7
Integrating rapid pathogen identification and antimicrobial susceptibility testing through multiplex TaqMan qPCR assay	7
Immunofluorescence detection of Ecytonucleospora hepatopenaei (EHP) in Penaeus vannamei	7
Integrated decentralized blood culture incubation: A step towards a 24/7 microbiology service?	7
Chemical susceptibility testing of non-tuberculous mycobacterium strains and other aquatic bacteria: Results of a study for the development of a more sensitive and simple method for the detection of N	7
Production of inactivated gram-positive and gram-negative species with preserved cellular morphology and integrity	7
Machine learning-based typing of Salmonella enterica O-serogroups by the Fourier-Transform Infrared (FTIR) Spectroscopy-based IR Biotyper system	7
Analysis of DNA extraction methods for detection of Treponema pallidum: A comparison of three methods	6
Antimicrobial susceptibility testing determined by Alfred 60/AST (Alifax®) in a multi-sites lab: performance's evaluation and optimization of workflow	6
Measurement uncertainty evaluation and risk of false conformity assessment for microbial enumeration tests	6
Quantitative method for measuring the proportion of bacterial cells expressing phase 1 or 2 of flagellin of Salmonella enterica serovar Typhimurium	6
Isolation of Harveyi clade Vibrio spp. collected in aquaculture farms: How can the identification issue be addressed?	6
Evaluating β-1,3-glucan synthesis inhibition using emulsion formation as an indicator	6
Editorial Board	6
Long-term storage stability of PGL-I coated ELISA plates for anti-Mycobacterium leprae IgM detection	6
Establishment of a TaqMan-MGB probe multiplex real-time PCR system for one-step levofloxacin and clarithromycin resistant Helicobacter pylori detection	6
Identification and validation of reference genes for reliable analysis of differential gene expression during antibiotic induced persister formation in Klebsiella pneumoniae using qPCR	6
Establishment and methodological evaluation of a rapid detection method for Cryptococcus neoformans and Cryptococcus gattii species complexes based on CRISPR-Cas12a technology	6
Agrobacterium tumefaciens-mediated transformation of Coniella granati	6
The future in diagnostic tools for TB outbreaks: A review of the approaches with focus on LAMP and RPA diagnostics tests	6
Comparative evaluation of indirect-ELISA and DOT blot assay for serodetection of Mycoplasma gallisepticum and Mycoplasma synoviae antibodies in poultry	6
Development of a new multiplex real-time PCR assay for rapid screening of hospital-acquired infection agents	6
Are GES carbapenemases underdiagnosed? An allelic discrimination assay for their accurate detection and differentiation	6
Labelling of eicosapentaenoic acid with stable isotope 13C in the marine bacterium Shewanella marinintestina	6
Phenotypic detection of carbapenemase production in carbapenem-resistant isolates with the rapid carbapenemase detection method (rCDM)	6
A comparison of total RNA extraction methods for RT-PCR based differential expression of genes from Trichoderma atrobrunneum	6
Antimicrobial potential of linear low-density polyethylene food packaging with Ag nanoparticles in different carriers (Silica and Hydroxyapatite)	6
Extraction of high-quality metagenomic DNA from the lichens Flavoparmelia caperata and Peltigera membranacea	6
Editorial Board	6
Meat extract casein peptone agar – A novel culture medium for the enumeration of Bacillus endospores in commercial products	6
Gene knock-out in Mycobacterium abscessus using Streptococcus thermophilus CRISPR/Cas	5
CAR1 as a new selective marker for genetic engineering of wine yeasts	5
Temporal modelling of the biofilm lifecycle (TMBL) establishes kinetic analysis of plate-based bacterial biofilm dynamics	5
Evaluation of a novel sample preparation method for identifying Aspergillus fumigatus using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: Combining Yatalase and silica	5
Development and validation of real-time PCR assays for the detection of Ehrlichia species and E. chaffeensis in clinical specimens	5
Enhanced insecticidal activity of Bacillus thuringiensis using a late embryogenesis abundant peptide co-expression system	5
Comparison of four enzymatic library preparation kits for sequencing Shiga toxin-producing Escherichia coli for surveillance and outbreak detection	5
Development of ELISA-based diagnostic methods for the detection of haemorrhagic septicaemia in animals	5
Study on antigenic protein Omp2b in combination with Omp31 and BP26 for serological detection of human brucellosis	5
In-house method for direct bacterial identification in positive blood culture broths using microfiltration, bead beating, and matrix-assisted laser desorption/ionization time-of-flight mass spectromet	5
Editorial Board	5
Validation of digital PCR assay for the quantification of Mycobacterium avium subsp. paratuberculosis in bovine faeces according to the ISO 20395:2019	5
Performance evaluation of InfectID-BSI: A rapid quantitative PCR assay for detecting sepsis-associated organisms directly from whole blood	5
Differentiation of Salmonella vaccinated and infected animals by serological detection of antibody to T3SS effector SsaK in an indirect ELISA	5
Incubation temperature and culture medium formulation impact the accuracy of pour-plate techniques for the enumeration of industrial Bacillus assemblages	5
Optimized methodology for obtention of high-yield and -quality RNA from the mycelium of the bioluminescent fungus Neonothopanus gardneri	5
Detection of hydrocarbon-degrading bacteria on deepwater corals of the northeast Atlantic using CARD-FISH	5
Improved probe detection sensitivity by flexible tandem biotin labeling	5
Editorial Board	5
Reporting off-target effects of recombinant engineering using the pORTMAGE system	5
PCR procedures to amplify GC-rich DNA sequences of Mycobacterium bovis	5
Evaluation of electroactive denitrifiers at different potentials, temperatures and buffers based on microcalorimetry	5
A rapid MALDI-TOF mass spectrometry-based method for categorization of Pseudomonas aeruginosa from blood cultures as susceptible or resistant to meropenem	5
Community structure and abundance of ACC deaminase containing bacteria in soils with 16S-PICRUSt2 inference or direct acdS gene sequencing	5
Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment	5
Routine laboratory test enabling the detection of dermatophytes and the identification of Trichophyton rubrum by means of in-house duplex real-time PCR	5
Establishing a quantitative index of meropenem hydrolysis for the detection of KPC- and NDM-producing bacteria by MALDI-TOF MS	5
Nucleus-directed fluorescent reporter system for promoter studies in the ectomycorrhizal fungus Laccaria bicolor	5
Development and evaluation of a novel quenching probe PCR (GENECUBE) assay for rapidly detecting and distinguishing between Chlamydia pneumoniae and Chlamydia psittaci	5
Editorial Board	5
Establishing the lower bacterial concentration threshold for different optical counting techniques	5
Development of an in vitro biofilm model of the human supra-gingival microbiome for Oral microbiome transplantation	4
A simple, sensitive, and specific method for the extraction and determination of thiamine and thiamine phosphate esters in fresh yeast biomass	4
Diagnosis of brucellosis disease using data mining: A case study on patients of a hospital in Tehran	4
Single-round multiplex PCR with species-specific mitochondrial primers of P. falciparum, P. vivax/P. simium and P. malariae/P. brasilianum: Comparison with standard techniques	4

Interlaboratory evaluations of a standardized quantitative test method for determining the bactericidal and tuberculocidal efficacy of antimicrobial substances on hard non-porous surfaces	4
Development and evaluation of a gold nanoparticle based Lateral Flow assay (LFA) strip test for detection of Brucella spp.	4
Non-amplification nucleic acid detection with thio-NAD cycling	4
An experimental setup and segmentation method for CFU counting on agar plate for the assessment of drinking water	4
Rapid preparation of 6S RNA-free B. subtilis σA-RNA polymerase and σA	4
A novel procedure for CFU plating and counting	4
Colorimetric strategies applicable for loop-mediated isothermal amplification	4
Evaluation and optimization of lysis method for microbial DNA extraction from epiphytic phyllosphere samples	4
Exploring the value of MALDI-TOF MS for the detection of clonal outbreaks of Burkholderia contaminans	4
Standardization of quantitative PCR (qPCR) method to detect the level of parasitaemia in Babesia gibsoni infected dogs	4
Spectinomycin, gentamicin, and routine disc diffusion testing: An alternative for the treatment and monitoring of multidrug-resistant Neisseria gonorrhoeae?	4
Development of a semi-dynamic in vitro model and its testing using probiotic Bacillus coagulans GBI-30, 6086 in orange juice and yogurt	4
Diversity and composition of gut microbiome of cervical cancer patients: Do results of 16S rRNA sequencing and whole genome sequencing approaches align?	4
Comparison of peaks in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of Staphylococcus aureus grown on various blood agar plates	4
Cryptosporidium occultus in disguise	4
Editorial Board	4
Simple and reliable in situ CRISPR-Cas9 nuclease visualization tool is ensuring efficient editing in Streptomyces species	4
Editorial Board	4
Highly sensitive quantification of bacterial chondroitin in a culture based on ELISA techniques	4
Standardization of in vitro dual-species biofilms of Staphylococcus pseudintermedius and Malassezia pachydermatis: a strategy to establish an ex vivo biofilm model	4
Transposition of insertion sequences by dielectric barrier discharge plasma and gamma irradiation in the radiation-resistant bacterium Deinococcus geothermalis	4
A fluorescent aptasensor for the detection of Aflatoxin B1 by graphene oxide mediated quenching and release of fluorescence	4
Molecular detection of Rickettsia felis in common fleas in Greece and comparative evaluation of genotypic methods	4
Diagnosis of abdominal tuberculosis: Detection of mycobacterial CFP-10 and HspX proteins by gold nanoparticle-PCR amplified immunoassay	4
Bacterial filtration efficiency of medical face masks – Evaluation of the use of disposable plastic petri dishes in Andersen impactor	4
A LAMP-based system for rapid detection of eight common pathogens causing lower respiratory tract infections	4
Simultaneous quantitation of 3ADON and 15ADON chemotypes of DON-producing Fusarium species in Chinese wheat based on duplex droplet digital PCR assay	4
Bacterial worth in genotoxicity assessment studies	4
Validation of gavage sampling as tool for longitudinal sampling of microbiota of the mouse gastric lumen	4
Determination of streptomycin and doxycycline using LC/MS towards an effective treatment against an experimental Brucella abortus infection in mice	4
An agar-based bioassay for accurate screening of the total antioxidant capacity of lactic acid bacteria cell-free supernatants	4
Development of a three-panel multiplex real-time PCR assay for simultaneous detection of nine canine respiratory pathogens	4
Broth microdilution protocol for determining antimicrobial susceptibility of Legionella pneumophila to clinically relevant antimicrobials	4
Design of potentially universal SSU primers in myxomycetes using next-generation sequencing	4
Rapid detection of KPC-producing Klebsiella pneumoniae in China based on MALDI-TOF MS	4
Decolorization and phytotoxicity reduction of reactive blue 40 dye in real textile wastewater by active consortium: Anaerobic/aerobic algal-bacterial-probiotic bioreactor	4
Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector	4
Target-enriched sequencing enables accurate identification of bloodstream infections in whole blood	4