Journal of Microbiological Methods

Papers
(The TQCC of Journal of Microbiological Methods is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-12-01 to 2025-12-01.)
ArticleCitations
Quantitative method for measuring the proportion of bacterial cells expressing phase 1 or 2 of flagellin of Salmonella enterica serovar Typhimurium73
Hyphal induction in Candida albicans: Optimizing medium parameters to accelerate hyphal growth enhanced by GlcNAc51
Editorial Board51
Measurement of lactose concentration in milk by using engineered bacteria producing lycopene39
A new killing assay with the Caenorhabditis elegans PX627 mutant to assess the virulence of uropathogenic Escherichia coli strains32
Combination of the electronic nose with microbiology as a tool for rapid detection of Salmonella32
Evaluation of phenotypic methods for detection of polymyxin B-resistant bacteria30
Evaluation of colonization factors of ETEC isolated from diarrheal patients in Bangladesh: A comparative approach using multiplex PCR and dot blot immunoassay30
Engineering of a monitorable expression system to characterize β-lactamase genes in Enterobacteriaceae29
Development of a high-throughput method for processing sponge-stick samples to detect viable Bacillus anthracis spores26
Campylobacter fetus subspecies specific PCR assays inferred from comparative genomic analysis for accurate subspecies identification23
Rapid antibiotic sensitivity prediction in Pseudomonas aeruginosa using UV–vis-NIR spectroscopy and gray-box one-vs-all models21
Multienzyme isothermal rapid amplification (MIRA)-LFD assay for rapid detection of PEDV and PoRVA21
Quantitative detection of Ganodermati lucidum immunomodulatory protein-8 by a peptide-antigen-antibody sandwich ELISA20
Standardization of in vitro dual-species biofilms of Staphylococcus pseudintermedius and Malassezia pachydermatis: a strategy to establish an ex vivo biofilm model19
Validation of gavage sampling as tool for longitudinal sampling of microbiota of the mouse gastric lumen19
Development of qPCR methods to detect and quantify the novel Fusarium graminearum 3ANX chemotype variant19
Improved productivity and dye removal performance of Trametes versicolor pellets using rice husk as a co-substrate19
Non-amplification nucleic acid detection with thio-NAD cycling19
Evaluation of electroactive denitrifiers at different potentials, temperatures and buffers based on microcalorimetry18
Statistical optimization of amylase production from Bacillus amyloliquefaciens using agro-industrial waste (mango peels) under submerged fermentation by response surface methodology18
Editorial Board18
Evaluation and optimization of lysis method for microbial DNA extraction from epiphytic phyllosphere samples18
Editorial Board18
Optimized methodology for obtention of high-yield and -quality RNA from the mycelium of the bioluminescent fungus Neonothopanus gardneri18
Diagnosis of abdominal tuberculosis: Detection of mycobacterial CFP-10 and HspX proteins by gold nanoparticle-PCR amplified immunoassay18
Performance evaluation of InfectID-BSI: A rapid quantitative PCR assay for detecting sepsis-associated organisms directly from whole blood17
Optimization of Manuka Honey microbubble particle structures for enhanced inhibition of Pseudomonas aeruginosa growth17
Spectinomycin, gentamicin, and routine disc diffusion testing: An alternative for the treatment and monitoring of multidrug-resistant Neisseria gonorrhoeae?16
Enterobacteriaceae in unpackaged ice cream: Implications for microbial safety and methodological concordance between ISO and TEMPO®16
Development of a three-panel multiplex real-time PCR assay for simultaneous detection of nine canine respiratory pathogens15
Nanoparticle immuno-fluorescent probes as a method for detection of viable E. coli O157:H715
A simple, sensitive, and specific method for the extraction and determination of thiamine and thiamine phosphate esters in fresh yeast biomass14
Innovative antigens for more accurate diagnosis of Q fever14
An improved multiplex PCR for Actinobacillus pleuropneumoniae, Glaesserella australis and Pasteurella multocida14
A comparative study of a rapid phenotypic antimicrobial susceptibility testing system directly from positive blood cultures to the disk diffusion and VITEK 2 methods14
Corrigendum to “Visualisation of Mycobacterium avium subsp. paratuberculosis in cultured cells, infected sheep and human tissue sections using fluorescent in situ hybridization (FISH)” [Journal of Mic14
Cryptosporidium occultus in disguise14
Standardisation and validation of an in-house quantitative real-time polymerase chain reaction (qPCR) assay for the diagnosis of Clostridioides difficile infection14
Evaluation of the BL-RED (Beta-Lactamase Rapid Electrochemical Detection) test for the rapid detection of resistance to third-generation cephalosporins in Enterobacteriaceae13
Editorial Board13
Quantification of cyanobacterial cyclic di-guanosine monophosphate (c-di-GMP) by liquid chromatography electrospray ionization tandem mass spectrometry13
Multi-dimensional nanoscale liquid chromatography and nano-electrospray ion-trap mass spectrometry for detection of Clostridium botulinum type C and the produced botulinum neurotoxin type C complex13
Editorial Board13
Short communication: Investigating optimal laboratory growth conditions of Gracilibacillus halotolerans in media supplemented with salt13
Development and validation of multiplex PCR based molecular serotyping of Salmonella serovars associated with poultry in India13
Electron microscopic observation of Mycobacterium avium subsp. paratuberculosis in cattle feces after a novel purification using liquid paraffin12
Development of polymerase chain reaction assays specific for individual pathogenic Aeromonas species12
Evaluation of anti-LipL32 carbon nanotube immunofluorescence probe (carbo-lip) and comparison with MAT, IgM ELISA, IgM spot test and culture for early detection of leptospirosis at local hospital12
Machine learning powered profiling: Rapid identification of Klebsiella Pneumoniae drug resistance from MALDI-TOF MS12
An improved MTT colorimetric method for rapid viable bacteria counting11
Preservation of Streptococcus pneumoniae quality control strains using chocolate agar blocks in glycerol at minus 70 degrees celsius11
CLI: A new protocol for the isolation of Lactic Acid Bacteria from complex plant samples11
Improving high-throughput techniques for bacteriophage discovery in multi-well plates11
A novel anti-C6 based approach for IgG avidity testing in Lyme borreliosis: A proof-of-concept study11
Editorial Board11
Rapid method for detection of Vibrio cholerae from drinking water with nanomaterials enhancing electrochemical biosensor11
A rapid in-lab toolbox for the discrimination of Vibrio natriegens and Escherichia coli cultures10
A simple method for purification of epsilon toxin of Clostridium perfringens type D for serum neutralization assay10
Evaluation of cobas® Liat® Cdiff, STANDARD™ M10 C. difficile and Xpert® C. difficile BT assays for rapid detection of toxigenic Clostridioides difficile10
A novel biosensing strategy for identification of three important bacteria causing meningitis10
Site-2 protease-like protein drives organic solvent tolerance in Rhodococcus ruber10
Syringe filter-based DNA aptamer-enzyme-linked colorimetric assay of Salmonella on lettuce10
High-throughput screening assay for nitrification inhibitors and the discovery of goitrin as a biological nitrification inhibitor10
Editorial Board10
Comparison of available methods to evaluate cefiderocol susceptibility in Acinetobacter spp10
Molecular detection of “Candidatus Rickettsia tarasevichiae” by Loop-mediated Isothermal Amplification (LAMP) of the ompA gene10
Application of proper orthogonal decomposition for evaluation of coherent structures and energy contents in microbial biofilms10
Performance of four bacterial cell counting apps for smartphones10
Cell-free synthesis of the Salmonella specific broad host range bacteriophage, felixO110
Editorial Board9
Development of a molecular marker for the Pi1 gene based on the association of the SNAP protocol with the touch-up gradient amplification method9
Revisiting gas-chromatography/mass-spectrometry molar response factors for quantitative analysis (FID or TIC) of glycosidic linkages in polysaccharides produced by oral bacterial biofilms9
A layered solid-state culture system for investigating the fungal growth and decay behaviour on the cellulosic substrate9
ClustFinder: A tool for threshold-delineated clustering of microbial isolates by pairwise genomic distance8
Long-term preservation and genetic stability of entomopathogenic fungal species8
Braided silk sutures coated with photoreduced silver nanoparticles for eradicating Staphylococcus aureus and Streptococcus mutans infections8
Pancreatic stone protein levels accurately predict severity in sepsis of various causes earlier than other biomarkers8
Investigation of the potential of yeast strains for phytase biosynthesis in a two-step screening procedure8
Green synthesis, characterization of silver nanoparticals for biomedical application and environmental remediation8
Identification of genus Deinococcus strains by PCR detection using the gyrB gene and its extension to Bacteria domain8
Genotyping of Corynebacterium pseudotuberculosis isolates using PCR-based DNA fingerprinting methods8
Disk-based method for rapid determination of primary antituberculosis drug susceptibility in Mycobacterium tuberculosis8
A new micro-agar dilution method to determine the minimum inhibitory concentration of essential oils against microorganisms8
Bioremediation of oily seawater by Bacteria immobilization on a novel carrier material containing nutrients8
Microcalorimetric growth behavior of E. coli ATCC 25922 in an MCDSC7
Methodological aspects of investigating the resistome in pig farm environments7
Evaluation of the clinical sensitivity and specificity of the BD Max™ Enteric Bacterial Panel for molecular detection of pathogens for acute gastroenteritis in the Singaporean population7
High-Resolution Melting (HRM) for rapid MLST analysis of Neisseria meningitidis7
Construction and optimization of a multiplex PMAxx-qPCR assay for viable Bacillus cereus and development of a detection kit7
Rapid qualitative identification and application of six common lactic acid bacteria based on surface enhanced Raman spectroscopy7
Integrated decentralized blood culture incubation: A step towards a 24/7 microbiology service?7
Cell-free production of fluorescent proteins for the discovery of novel ribosome-targeting antibiotics7
Classifying fungi biodiversity using hybrid transformer models7
Inhibition of Pseudomonas aeruginosa with silver in a new synthetic medium: Investigation on the MIC, growth rate and lag-phase at the lower limit7
Host specific Eimeria genus diagnosis and qPCR development in Ovis aries and Capra hircus7
Development of a novel visual isothermal amplification assay for rapid detection of Brucella spp.7
Identification of cry genes in Bacillus thuringiensis by multiplex real-time PCR7
A strip-based assay for detection of CrfA enzyme activity to differentiate Mycobacterium tuberculosis and non-tuberculous mycobacteria7
Assessment of the capacity of Whatman filter papers as support to store stools for the molecular diagnostic testing of soil-transmitted helminthiasis7
SensiGram as a modified Gram staining procedure with higher sensitivity for detection of Gram-negative bacteria in blood culture7
Editorial Board7
A variety of simple and ultra-low-cost methods preparing SLiCE extracts and their application to DNA cloning7
Investigating E, E-farnesol production unleashed by an indigenous Saccharomyces cerevisiae isolated from baker's yeast under stationary culture7
Crystal violet-based assay for the assessment of bacterial biofilm formation in medical tubing7
Engineering Citrobacter freundii using CRISPR/Cas9 system6
Estimation of biosurfactant production parameters and yields without conducting additional experiments on a larger production scale6
Differentiation of Salmonella vaccinated and infected animals by serological detection of antibody to T3SS effector SsaK in an indirect ELISA6
A rapid MALDI Biotyper based approach for screening anovaginal colonization by Streptococcus agalactiae in pregnant women6
Application of flow cytometry for rapid, high-throughput, multiparametric analysis of environmental microbiomes6
Editorial Board6
Coupon position does not affect Pseudomonas aeruginosa and Staphylococcus aureus biofilm densities in the CDC biofilm reactor6
In vivo Fusarium virulence and efficacy of voriconazole in a Galleria mellonella model of invasive fusariosis6
Development of real-time PCR methods for the quantification of Methanoculleus, Methanosarcina and Methanobacterium in anaerobic digestion6
Transcriptomic insights into biofilm dynamics and therapeutic targets in chronic wound infections (MIMET 107281)6
A study of various factors affecting satellitism tests of Haemophilus influenzae and Haemophilus parainfluenzae using Staphylococcus aureus as the source of NAD6
Insight into the biotechnology potential of Alicyclobacillus tolerans from whole genome sequence analysis and genome-scale metabolic network modeling.6
Broth microdilution protocol for determining antimicrobial susceptibility of Legionella pneumophila to clinically relevant antimicrobials6
Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment6
Prospective study on combined use of Sabouraud dextrose agar and blood agar for improved recovery of etiological agents in mycotic and Pythium keratitis6
Phenotypic detection of carbapenemase production in carbapenem-resistant isolates with the rapid carbapenemase detection method (rCDM)6
The performance of a Fully Automated Urine Particle Analyzer, Sysmex UF-5000, in detecting fastidious bacteria in urine samples6
Systematic review and critical reflection on the isolation and identification methods for spoilage associated bacteria in fresh marine fish6
Editorial Board6
Comparison of molecular serotyping methods for Actinobacillus pleuropneumoniae and analysis of atypical serotypes detected in routine diagnostics6
Rapid detection of KPC-producing Klebsiella pneumoniae in China based on MALDI-TOF MS6
Sequencing on the edge: Tools and techniques for sequencing low biomass isolates from unicellular eukaryotic parasites and other challenging samples6
Adapting a 96-well liquid culture assay to a colonial growth confirmation assay utilizing a 3D printed guide-frame system for a 96-well replicator6
Advances in molecular diagnostics of Neisseria gonorrhoeae6
The future in diagnostic tools for TB outbreaks: A review of the approaches with focus on LAMP and RPA diagnostics tests6
Deciphering microbial diversity and predicting metabolic functionalities in fermented pigmented rice water using culture-independent characterization6
Electrochemical based quantification of bacterial growth in media using square wave voltammetry and low cost screen printed electrodes5
Comparing DNA isolation and sequencing strategies for 16S rRNA gene amplicon analysis in biofilm containing environments5
Rapid counting of Kazachstania humilis and Saccharomyces cerevisiae in sourdough by deep learning-based classifier5
Biomass and metabolic activity staining biofilm techniques are not reliable enough to be used in microbiology laboratories5
Improvement of the silver staining method for bacterial flagella5
Editorial Board5
Studying the pre-implantation uterine microbiota in cattle using transabdominal laparoscopic low-volume lavage: Aiming for zero-contamination5
A practical method for gas changing time estimation using a simple gas-liquid mass transfer model5
Electrotransformation of thermophilic bacterium Caldimonas manganoxidans5
Novel standard biodegradation test for synthetic phosphonates5
Follow-up of solid-state fungal wood pretreatment by a novel near-infrared spectroscopy-based lignin calibration model5
Effects of microbial inoculants on soil microbial communities and enhancement of tomato yield5
Editorial Board5
A multistep enrichment process with custom growth medium improves resuscitation of chlorine-stressed coliforms from secondary sewage effluents5
Application of a rapid and sensitive RPA-CRISPR/Cas12a assay for BCSP31-based Brucella detection5
Single primer site-specific nested PCR for accurate and rapid genome-walking5
Bacterial growth comparison of vaccine and non-vaccine type Streptococcus pneumoniae in different enrichment broths5
Anionic nanocellulose as competing agent in microbial DNA extraction from mine process samples5
I-dOne: A diagnostic tool in the field of identification of clinically relevant microbial strains5
A new plate-hanging method for biofilm quantification and its application to evaluate the role of surface hydrophobicity5
Curing of common plasmids in gram-negative bacteria using a Cas9-based conjugative vector5
Quantitative investigation of the bacterial content of periodontal abscess samples by real-time PCR5
Validation of pleural fluid group A Streptococcus and Staphylococcus aureus PCR assays and their potential clinical impact in children with complicated pneumonia5
Subtractive genomics approach: A guide to unveiling therapeutic targets across pathogens5
Evaluation of a nucleic acid amplification system, GENECUBE, for rapid detection of staphylococcal nuc and mecA in blood culture samples5
Microbiome analysis from formalin-fixed paraffin-embedded tissues: Current challenges and future perspectives5
Identification of bacterial infection types in decomposition stages at various temperatures using pathology images and artificial intelligence algorithms5
New vectors and optimal conditions for allelic exchange in hypervirulent Klebsiella pneumoniae5
Standardized ready-to-use laboratory protocol to isolate primary human wound associated fibroblasts from infected wound samples for clinical applications5
A new duplex qPCR-based method to quantify Mycoplasma mycoides in complex cell culture systems and host tissues5
A replica plating method for efficient, high-throughput screening of antibiotic gene clusters in bacteria uncovers a holomycin-like cluster in the clinical isolate, Pantoea agglomerans 20KB4479735
Comparison of DNA/RNA yield and integrity between PMAP36-mediated and other bacterial lysis methods5
Leptospiral sphingomyelinase Sph2 as a potential biomarker for diagnosis of leptospirosis5
Efficiency of CRISPR-Cas9 genetic engineering in Escherichia coli BL21 is impaired by lack of Lon protease5
Design and validation of universal and taxon-specific 16S rRNA qPCR primers for detection of freshwater harmful algal bloom-forming cyanobacteria5
A workflow for selective isolation of bacterial RNA from Streptococcus agalactiae during in vivo infection4
Occurrence of Rhodococcus sp. RR1 prmA and Rhodococcus jostii RHA1 prmA across microbial communities and their enumeration during 1,4-dioxane biodegradation4
Rapid and specific detection of Mycoplasma gallisepticum and Mycoplasma synoviae infection in poultry using single and duplex PCR assays4
The AMR-ARRAY: A modular bead array detecting β-lactam, (fluoro) quinolone, colistin, aminoglycoside and macrolide resistance determinants in Gram-negative bacteria4
A European multicenter evaluation study to investigate the performance on commercially available selective agar plates for the detection of carbapenemase producing Enterobacteriaceae4
Development and validation of a bacterial gastrointestinal multiplex RT-PCR assay for use on a fully automated molecular system4
Current methodologies and future direction of Campylobacter isolation and detection from food matrices, clinical samples, and the agricultural environment4
Evaluation of a nucleic acid preservation protocol for microbiome studies involving samples from the oral cavity4
Editorial Board4
Multi-part strategy for testing differential taxa abundance in sequencing data: A simulation study with an application to a microbiome study4
High-throughput characterization of the filamentous cyanobacterium Anabaena sp. using flow cytometry4
Development and evaluation of a sensitive recombinase aided amplification assay for rapid detection of Vibrio parahaemolyticus4
Enhancing sensitivity of qPCR assays targeting Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae by using a mutant Taq DNA polymerase4
Comparative analysis of diagnostic assays for scrub typhus: Unveiling enhanced approaches for accurate detection4
Development of a closed-tube, calcein-based loop-mediated isothermal amplification assay to detect Salmonella spp. in raw meat samples4
Improving the identification of pneumonia pathogens by using the real-time polymerase chain reaction (qPCR) alongside culture methods4
Rapid identification of Salmonella serovars Enteritidis and Typhimurium using whole cell matrix assisted laser desorption ionization – Time of flight mass spectrometry (MALDI-TOF MS) coupled with mult4
Determination of limit of detection and relative limit of detection of Salmonella in raw pet food matrices using Salmonella bacteriological analytical manual methods4
Characterization of circulating Mycoplasma hyopneumoniae variants in the Midwestern United States using Multiple-Locus Variable number tandem repeat Analysis and P146 gene sequencing4
Novel method for screening probiotic candidates tolerant to human gastrointestinal stress4
Determination of foodborne pathogens in minced beef by real-time PCR without culture enrichment4
Alternative protocol leading to rapid identification of Actinomycetes isolated from Algerian desertic soil by MALDI-TOF mass spectrometry4
A study of the potential application of digital PCR in the detection of fecal contamination of strawberries using Bacteroides markers4
Development of a Streptococcus pluranimalium-specific PCR assay4
Identification of novel Listeria monocytogenes serotype 4h with specific antisera against wall teichoic acid4
Novel method to recover Salmonella enterica cells for Tn-Seq approaches from lettuce leaves and agricultural environments using combination of sonication, filtration, and dialysis membrane4
Current advancements in nanoparticles for vaccines and drug delivery for the treatment of tuberculosis4
Measurement of Babesia bovis infected red blood cells using flow cytometry4
Development and validation of a multi-target TaqMan qPCR method for detection of Borrelia burgdorferi sensu lato4
Improvement of transformation efficiency in hypermucoviscous Klebsiella pneumoniae using citric acid4
Effects of primers, PCR approaches and sample preservation methods on diversity studies of myxobacteria4
A new PMA-qPCR method for rapid and accurate detection of viable bacteria and spores of marine-derived Bacillus velezensis B-99874
The implications of quorum sensing inhibition in bacterial antibiotic resistance- with a special focus on aquaculture4
A panoptic review of techniques for finfish disease diagnosis: The status quo and future perspectives4
Development of a microplate absorbance assay for assessing fungicide sensitivity of filamentous fungi and comparison to an amended agar assay4
Leveraging artificial intelligence in vaccine development: A narrative review4
A single-container procedure for multiple genomic DNA samples preparation for pulsed field gel electrophoresis4
Streamlined construction of episomal vectors for rapid assessment of fusion phytase display in Saccharomyces cerevisiae4
Rapid screening of primary and rationally synthesized anti-mycobacterial compounds in macrophage using double recombinant M. bovis BCG strain4
Development of detection methods by multiplex real-time PCR for highly pathogenic Yersinia enterocolitica, low pathogenic Yersinia enterocolitica, and Yersinia pseudotuberculosis based on SYBR Green a4
Editorial Board4
Diagnostic potential of recombinant Mycobacterium tuberculosis PcaA antigen and its enhancement of protective efficacy as a subunit vaccine booster following BCG priming4
Systematic review of co-culture methods for studying vaginal biofilm formation in bacterial vaginosis4
Contribution to optimization and standardization of antibacterial assays with silver nanoparticles: the culture medium and their aggregation4
Understanding the reaction balances behind the viability PCR protocols based on photoreactive dyes4
Editorial Board4
A rapid and visual detection for canine Adenovirus-2 using CRISPR-Cas13a-based SHERLOCK technology4
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