Journal of Virological Methods

Article	Citations
Corrigendum to “PEG-8000 precipitation as a rapid method for enrichment and purification of feline calicivirus” [J. Virol. Methods 344 (2026) 1–6, 115390]	35
An indirect ELISA to detect antibodies to the gC of bovine alphaherpesvirus 1 (BoAHV1) displaying no crossreactivity with antibodies induced by bovine alphaherpesvirus 5 (BoAHV5)	25
Editorial Board	20
The development of a rapid, high-throughput neutralization assay using a SARS-CoV-2 reporter	20
Development of end-point and quantitative polymerase chain reaction assays to detect and discriminate between blueberry scorch virus and blueberry virus S	19
Clinical validation of the Roche cobas HPV test on the Roche cobas 6800 system for the purpose of cervical screening and qualification as a second-generation comparator test	19
Metagenomic surveillance of emerging viruses in mosquito populations from high-risk regions of Iran	19
Implication of cell culture methods and biases on UV inactivation of viruses	16
Fully automated sample-to-answer PCR detection of severe fever with thrombocytopenia syndrome virus in cat serum and whole blood	16
Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction	16
Analysis of the antigenic and immunogenic properties of the native rabies virus glycoprotein purified by Lens culinaris lectin affinity chromatography	15
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples	15
Investigation of atypical serological profiles for Epstein-Barr virus (EBV)	14
Development of an ARMS-Quadruplex-qPCR assay for the rapid identification of MPXV and the clades Ia, Ib, IIa and IIb	14
Editorial Board	14
Comparison of EBOV GP IgG antibody reactivity: Results from two immunoassays in the Democratic Republic of the Congo	14
Editorial Board	14
Real-time detection of cucurbit chlorotic yellows virus by RT-LAMP	14
Rapid identification of SARS-CoV-2 variants: Validation of the simplexa SARS-CoV-2 variant direct assay	13
Tween 80 improves the infectivity of BCL1 cell-adapted infectious bursal disease virus	13
Detecting rubella virus-specific antibodies by cytopathic effect and immunoenzymatic microneutralization tests	13
Development and validation of a highly specific in-house chemiluminescent-based serological assay for the detection of antibodies directed against the human monkeypox virus	13
Generation and application of immortalized sertoli cell line from sheep testis	13
Discrepant results of hepatitis B virus genotype determination by PCR and DNA sequencing	12
Performance evaluation of TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV) for detection and differentiation of dengue and chikungunya viral RNA in serum samples of symptomatic patients	12

A systematic review on current approaches in bat virus discovered between 2018 and 2022	12
LSDV126 gene based molecular assays for specific detection and characterization of emerging Lumpy Skin Disease virus	12
The development of RT-RPA and CRISPR-Cas12a based assay for sensitive detection of infectious hematopoietic necrosis virus (IHNV)	12
A viral metagenomic protocol for nanopore sequencing of group A rotavirus	12
Diagnosis of Monkeypox infection: Validation of two diagnostic kits for viral detection using RT-PCR	11
Double testing with lateral flow antigen test devices for COVID-19: does a second test in quick succession add value?	11
Concordance in RT-PCR detection of SARS-CoV-2 between samples preserved in viral and bacterial transport medium	11
Alternatives to central nervous system samples for enhanced rabies surveillance: Exploring natural infections of Lyssavirus rabies in striped skunks (Mephitis mephitis) in Northern Colorado	11
Purification and characterization of kyasanur forest disease virus EDIII domain of major envelope glycoprotein	11
Development of sensitive and specific indirect ELISA tests for the detection of antibodies against bovine leukemia virus in serum and milk samples	11
Developing Zika virus-transduced hACE2 expression models for severe acute respiratory syndrome coronavirus 2 infection in vitro and in vivo	11
Development and application of a real-time RT-PCR assay for the specific detection of influenza D virus	10
A split GFP approach to assay SARS-CoV-2 spike-dependent cell fusion	10
Evaluation of inactivated snakehead rhabdovirus as an internal positive control for RT-qPCR diagnosis of viral hemorrhagic septicemia virus in fish	10
Corrigendum to “Targeted enrichment of elephant endotheliotropic herpesvirus for complete genome sequencing in elephants” [J. Virol. Methods 338 (2025), 115230]	10
Screening of monoclonal vaccine strains based on real-time live-cell imaging technology	10
An optimised protocol for the expression and purification of adenovirus core protein VII	10
Quantitative real time PCR detection of Saboya virus: A flavivirus member of yellow fever genetic group	10
Subtype specific virus enrichment with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus	10
Investigation of variants in genetics and virulence of Porcine Epidemic Diarrhea Virus after serial passage on Vero cells	9
Optimization of rabies virus production in neuroblastoma cells using quality by design principles for vaccine and diagnostic applications	9
Evaluation of the flinders technology associates cards for storage and molecular detection of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) DNA	9
Development and preliminary validation of a MERS-CoV ELISA for serological testing of camels and alpacas	9
Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring	9
Development and validation of an all-in-one rabies virus Bat-Clade genomic sequencing and host identification protocol	9
Disassembly mediated multimodal chromatography based purification of HPV-VLPs produced in Pichia pastoris	9
Evaluation of filter paper to transport oro/nasopharyngeal samples to detect SARS-CoV-2 by RT-qPCR	9
Evaluation of two commercial multiplex RT-PCR tests for broad genotypic detection of viral gastroenteritis	9
Evaluation of a concentration method for the recovery of human adenovirus from mineral water, tap water and well water	9
Editorial Board	9
Evaluation of the impact of hollow fiber pore size and membrane material on virus concentration using a handheld hollow fiber method	9
IC-ELISAs for the quantitative detection of monkeypox virus cross-immunogenic modified vaccinia virus Ankara antigens L1 and A33	9
Inactivation of airborne SARS-CoV-2 by thyme volatile oil vapor phase	8
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa	8
Protocol for virome characterization in low-volume respiratory samples from broiler chickens	8
The optimisation and application of a novel BaseScope™ RNA-ISH assay for the detection of foot-and-mouth disease virus in carrier African buffalo (Syncerus caffer) from South Africa	8
Cryopreservation of chicken and duck tracheal rings and precision-cut lung slices: A promising tool for the rapid characterization of avian influenza viruses	8
Prokaryotic expression of porcine deltacoronavirus S gene truncated segment and establishment of indirect ELISA detection method	8
Development of a sensitive real-time quantitative RT-PCR assay for the detection of pear chlorotic leaf spot-associated virus	8
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India	8
Preparation and evaluation of IgY against human papillomavirus	8
High-dimensional droplet digital PCR of multiple hepatitis B splice variants in serum, liver, and tissue culture	8
Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (RPA-LFA)	8
Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays	8
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus	8
Adjusting susceptibilities of C57BL/6 mice to orthoflaviviruses for evaluation of antiviral drugs by altering the levels of interferon alpha/beta receptor function	8
Development of a duplex LNA-TaqMan real-time quantitative PCR for differential detection of virulent and attenuated strains of Muscovy duck-origin goose parvovirus	8
Inactivation of infectious virus samples through UV irradiation for downstream analysis	8
Real time PCR-based evaluation of live attenuated lumpy skin disease virus vaccines for immunogenicity and efficacy	8
Effective plant virus enrichment using carbon nanotubes and microfluidics	8

The co-application of nectin-1 and PRV gD monoclonal antibodies: An effective approach to block pseudorabies virus invasion	7
Rapid detection of porcine encephalomyocarditis virus (EMCV) by isothermal reverse transcription recombinase polymerase amplification assays	7
Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes	7
Development and validation of a DIVA ELISA for differentiating BEFV infected from vaccinated animals	7
Optimizing virus inactivation methods for molecular detection techniques: Implications for viral protein and RNA measurements	7
Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection	7
Rapid metagenomic identification of two major swine pathogens with real-time nanopore sequencing	7
Duplex droplet digital PCR enables simultaneous quantification of algal giant virus DSLLAV1 and virophage DSLV8 in natural and laboratory samples	7
Development of an accurate and rapid method for whole genome characterization of canine parvovirus	7
Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification	7
Editorial Board	7
Insights into antiviral activity of chlorpromazine against RNA viruses: Molecular docking, ADME profile, and semi-in vivo study	7
Establishment of a fetal cow (Bos Borus) skin fibroblasts cell line with immortalized characterization through human telomerase reverse transcriptase (hTERT) ectopic expression	7
Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses	7
AI-driven strategies for enhancing Mpox surveillance and response in Africa	7
SARS-CoV-2-specific T cell responses: a comparative analysis between QuantiFERON SARS-CoV-2, T-SPOT.COVID, and an in-house Omicron ELISpot	7
Editorial Board	7
Chemical inactivation of foot-and-mouth disease virus in bovine tongue epithelium for safe transport and downstream processing	7
One-step TaqMan® RT-qPCR detection of sugar beet-infecting poleroviruses in Myzus persicae from yellow water pan traps opens up new possibilities for early risk assessment of virus yellows disease	6
Potential of recombinant avian adeno-associated virus as a viral vector for CRISPR/Cas9 delivery to avian cells	6
Effect of time and temperature on the stability of HPV and cellular nucleic acid using simulated dry self-samples	6
Development and validation of reverse-transcription cross-priming amplification-based lateral flow assay for the detection of infectious hematopoietic necrosis virus	6
Characterization of monoclonal antibodies targeting SARS-CoV-2 spike glycoprotein: Reactivity against Delta and Omicron BA.1 variants	6
Efficiency of CMV serodiagnosis during pregnancy in daily laboratory routine	6
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV	6
Detection of antibodies against infectious bronchitis virus strain QX (GI-19) by commercial ELISA kits and virus neutralization test	6
A novel methodology for assessing contact tracing precision: Phylogenetic validation of a contact tracing program for COVID-19 in Belgium	6
Potential use of high-resolution melting analyses for SARS-CoV-2 genomic surveillance	6
Simultaneous detection of five viruses and two viroids affecting apples through a DNA macroarray chip	6
Comparison of Polymerase Chain Reaction (PCR) assay performance in detecting Decapod penstylhamaparvovirus 1 in penaeid shrimp	6
Rapid and efficient generation of viral genome knock-in cell lines using the CRISPR-Cas9 system to produce infectious virus	6
Design, development, and validation of a strand-specific RT-qPCR assay to differentiate replicating versus nonreplicating Rabies virus	6
Saliva samples in SARS-Cov-2 virus detection compared to the nasopharyngeal RT-PCR findings in individuals with suspected COVID-19 infection	6
Comparison of sample pretreatments used to distinguish between infectious and non-infectious foodborne viruses by RT-qPCR	6
A double-strain TM (gp45) polypeptide antigen and its application in the serodiagnosis of equine infectious anemia	6
Simultaneous expression of three reporter proteins from a porcine reproductive and respiratory syndrome virus-based vector	6
A thermal cycler-based, homogenization-free plant total nucleic acid extraction method for plant viruses and viroids assay	6
Development of a novel multiplex digital PCR-based method for the detection of HTLV-1 proviral deletion	6
Comparing methods to detect cellular proteins on the surface of HIV-1 virions	5
Development of an indirect ELISA test for the detection of Tilapia lake virus (TiLV) in fish tissue and mucus samples	5
Single-domain antibodies applied as antiviral immunotherapeutics	5
A novel microarray-based PCR assay for the detection of HSV-1, HSV-2, and VZV skin infections: A retrospective analysis	5
Development of an in-house Indirect ELISA for detection of bovine viral diarrhoea virus antibodies in bovine sera	5
MALDI-TOF nucleic acid mass spectrometry for simultaneously detection of fourteen porcine viruses and its application	5
A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine	5
Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV)	5
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region	5
Editorial Board	5
Validation of an HPLC-CAD method for measuring the lipid content of novel LNP-encapsulated COVID-19 mRNA vaccines	5
Assessing the efficacy of different bead-based assays in capturing hepatitis E virus	5
m-PIMA™ HIV1/2 VL: A suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa	5
Hybrid next-generation sequencing protocol for testing HIV-2 drug resistance	5
Antiviral effect of an extract from Kaempferia galanga L. rhizome in mice infected with pseudorabies virus	5
Development and applications of a TaqMan based quantitative real-time PCR for the rapid detection of Pigeon circovirus (PiCV)	5
Fully automated dried blood spot sample handling and extraction for BoHV-1 antibody testing by ELISA	5
Development of In Vitro assay for viral escape from broadly neutralizing antibodies	5
Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening	5
Preliminary establishment and validation of a loop-mediated isothermal amplification assay for convenient screening of 13 types of high-risk human papillomaviruses in cervical secretions	5
Comparative analysis of RT-qPCR assay sensitivity and process limit of detection for Japanese encephalitis virus (JEV) detection in piggery wastewater	5
‘CoviSwiftTM’: A point-of-care RT-PCR device for SARS-CoV-2 and its variant detection	5
Product Enhanced Reverse Transcriptase for assessing replication competent virus in vectors retroviral vectors pseudotyped with GALV and VSV-G envelopes	5
Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples	5
A rapid immunochromatographic assay for the detection of BK Polyomavirus in urine samples from hematopoietic stem cell transplant recipients	5
Evaluating the effectiveness of a novel environmental decontamination system utilizing low-energy hyper-charged photoelectrons against coronavirus	5
Development of a one-step RT-qPCR assay for the detection of Grapevine leafroll-associated virus 7.	5
Role of human cytomegalovirus in the pathogenesis of periapical lesions – A systematic review and meta-analysis	5
A new real-time RT-PCR for the detection of piscine orthoreoviruses PRV-1 and PRV-3	5
Development of a validated molecular analytical method to determine the viral safety of F(AB´)2 products: A novel application for a well-known technique	5
Editorial Board	5
Editorial Board	4
Indirect ELISA for analysis of malignant catarrhal fever virus-specific antibodies in a range of species	4
Development of one step colorimetric RT-LAMP assays for rapid detection of Apple mosaic virus and Prunus necrotic ringspot virus	4
Performance evaluation of a SYBR Green-based real-time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples	4
Establishment of a rapid real-time fluorescence-based recombinase-aided amplification method for detection of avian infectious bronchitis virus	4
Development of a one-step RT-qPCR assay for the detection of Citrus concave gum-associated virus in apples	4
Editorial Board	4
Development of two competitive ELISAs based on monoclonal antibodies for the serological detection of Bovine ephemeral fever virus	4
Monoclonal antibody based solid phase competition ELISA to detect FMDV serotype A specific antibodies	4
A multiplexed real‐time PCR assay for simultaneous quantification of human immunodeficiency virus and Hepatitis B virus for low‐and‐middle‐ income countries	4
Development of transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay for visual detection of citrus tatter leaf virus	4

Rapid detection of the emerging tick-borne Tamdy virus by TaqMan-based real-time reverse transcription PCR	4
An automated assay platform for the evaluation of antiviral compounds against polioviruses	4
In planta expression of specific single chain fragment antibody (scFv) against nucleocapsid protein of fig mosaic virus (FMV)	4
Genetic influence of IFN-γ gene polymorphisms on hepatitis C progression and recovery	4
A validated in-house assay for HIV drug resistance mutation surveillance from dried blood spot specimens	4
Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus	4
Establishment and application of multiplex PCR for rapid detection of three mink diarrhea-associated viruses	4
Specific antibody production using recombinant proteins to elucidate seed transmission and nuclear localization of Coguvirus citrulli and Coguvirus henanense in radicles of watermelon crop	4
Combined nasal- and oropharyngeal self-swab provides equivalent performance compared to professionally collected oropharyngeal swabs in detecting SARS-CoV-2 in a real-life setting	4
Critical parameters on Zika virus-like particles’ generation	4
A novel digital PCR assay for detection and comprehensive characterization of Molluscum contagiosum virus genotypes MOCV1, MOCV2, and MOCV3 and recombinant lineages	4
Application of digital PCR and CRISPR/Cas13a-based fluorescent assay for accurate and on-site detection of cotton leafroll dwarf virus	4
Editorial Board	4
Comparing solid-based concentration methods for rapid and efficient recovery of SARS-CoV-2 for wastewater surveillance	4
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid test for PPRV antibody	4
Effective RNA extraction with readily available reagents: A comparative analysis of alternative protocols	4
Effects of porcine epidemic diarrhoea virus vaccination timing and route of administration on colostrum antibody levels and prevention of diarrhoea in piglets on a pig farm with porcine epidemic diarr	4
Validation of laboratory developed serology assays for detection of IgG antibody to severe acute respiratory syndrome coronavirus 2 in the South African population	4
Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing	4
A hydroxypropyl methylcellulose plaque assay for human respiratory syncytial virus	4
Two immune-based methods using immortalized porcine kidney macrophages for quantifying neutralizing activity against porcine reproductive and respiratory syndrome virus-2	4
Evaluation of PCR-based hemagglutinin subtyping as a tool to aid in surveillance of avian influenza viruses in migratory wild birds	4
Comparison of the AmpFire and MA-6000 polymerase chain reaction platforms for high-risk human papillomavirus testing in cervical precancer screening	4
A simplified method of bacteriophage preparation for transmission electron microscope	4