Journal of Virological Methods

Article	Citations
Clinical validation of the Roche cobas HPV test on the Roche cobas 6800 system for the purpose of cervical screening and qualification as a second-generation comparator test	38
Editorial Board	25
The development of a rapid, high-throughput neutralization assay using a SARS-CoV-2 reporter	22
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples	20
Analysis of the antigenic and immunogenic properties of the native rabies virus glycoprotein purified by Lens culinaris lectin affinity chromatography	19
Investigation of atypical serological profiles for Epstein-Barr virus (EBV)	19
Fully automated sample-to-answer PCR detection of severe fever with thrombocytopenia syndrome virus in cat serum and whole blood	18
Implication of cell culture methods and biases on UV inactivation of viruses	18
Development of end-point and quantitative polymerase chain reaction assays to detect and discriminate between blueberry scorch virus and blueberry virus S	17
Corrigendum to “PEG-8000 precipitation as a rapid method for enrichment and purification of feline calicivirus” [J. Virol. Methods 344 (2026) 1–6, 115390]	17
An indirect ELISA to detect antibodies to the gC of bovine alphaherpesvirus 1 (BoAHV1) displaying no crossreactivity with antibodies induced by bovine alphaherpesvirus 5 (BoAHV5)	17
Editorial Board	15
Detecting rubella virus-specific antibodies by cytopathic effect and immunoenzymatic microneutralization tests	15
Editorial Board	15
Performance evaluation of TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV) for detection and differentiation of dengue and chikungunya viral RNA in serum samples of symptomatic patients	15
Metagenomic surveillance of emerging viruses in mosquito populations from high-risk regions of Iran	15
A systematic review on current approaches in bat virus discovered between 2018 and 2022	14
Rapid identification of SARS-CoV-2 variants: Validation of the simplexa SARS-CoV-2 variant direct assay	14
LSDV126 gene based molecular assays for specific detection and characterization of emerging Lumpy Skin Disease virus	14
Generation and application of immortalized sertoli cell line from sheep testis	14
Development and validation of a highly specific in-house chemiluminescent-based serological assay for the detection of antibodies directed against the human monkeypox virus	14
A viral metagenomic protocol for nanopore sequencing of group A rotavirus	13
Real-time detection of cucurbit chlorotic yellows virus by RT-LAMP	13
Development of an ARMS-Quadruplex-qPCR assay for the rapid identification of MPXV and the clades Ia, Ib, IIa and IIb	13
Development of sensitive and specific indirect ELISA tests for the detection of antibodies against bovine leukemia virus in serum and milk samples	13

Alternatives to central nervous system samples for enhanced rabies surveillance: Exploring natural infections of Lyssavirus rabies in striped skunks (Mephitis mephitis) in Northern Colorado	13
The development of RT-RPA and CRISPR-Cas12a based assay for sensitive detection of infectious hematopoietic necrosis virus (IHNV)	12
Development and validation of a one-step RT-ddPCR assay for sensitive and absolute quantification of foot-and-mouth disease virus RNA in probang fluid	12
Comparison of EBOV GP IgG antibody reactivity: Results from two immunoassays in the Democratic Republic of the Congo	12
Double testing with lateral flow antigen test devices for COVID-19: does a second test in quick succession add value?	12
Diagnosis of Monkeypox infection: Validation of two diagnostic kits for viral detection using RT-PCR	12
Quantitative real time PCR detection of Saboya virus: A flavivirus member of yellow fever genetic group	11
Screening of monoclonal vaccine strains based on real-time live-cell imaging technology	11
A split GFP approach to assay SARS-CoV-2 spike-dependent cell fusion	11
Purification and characterization of kyasanur forest disease virus EDIII domain of major envelope glycoprotein	11
An optimised protocol for the expression and purification of adenovirus core protein VII	11
Developing Zika virus-transduced hACE2 expression models for severe acute respiratory syndrome coronavirus 2 infection in vitro and in vivo	11
Corrigendum to “Targeted enrichment of elephant endotheliotropic herpesvirus for complete genome sequencing in elephants” [J. Virol. Methods 338 (2025), 115230]	11
Evaluation of inactivated snakehead rhabdovirus as an internal positive control for RT-qPCR diagnosis of viral hemorrhagic septicemia virus in fish	11
Development and application of a real-time RT-PCR assay for the specific detection of influenza D virus	11
Editorial Board	10
Evaluation of two commercial multiplex RT-PCR tests for broad genotypic detection of viral gastroenteritis	10
Disassembly mediated multimodal chromatography based purification of HPV-VLPs produced in Pichia pastoris	10
Optimization of rabies virus production in neuroblastoma cells using quality by design principles for vaccine and diagnostic applications	10
Subtype specific virus enrichment with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus	10
Investigation of variants in genetics and virulence of Porcine Epidemic Diarrhea Virus after serial passage on Vero cells	10
Evaluation of the flinders technology associates cards for storage and molecular detection of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) DNA	10
Rethinking classical swine fever virus phylogenetics: NS3 Outperforms traditional gene markers	10
Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring	10
Evaluation of a concentration method for the recovery of human adenovirus from mineral water, tap water and well water	9
Preparation and evaluation of IgY against human papillomavirus	9
Adjusting susceptibilities of C57BL/6 mice to orthoflaviviruses for evaluation of antiviral drugs by altering the levels of interferon alpha/beta receptor function	9
Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (RPA-LFA)	9
Development of a duplex LNA-TaqMan real-time quantitative PCR for differential detection of virulent and attenuated strains of Muscovy duck-origin goose parvovirus	9
IC-ELISAs for the quantitative detection of monkeypox virus cross-immunogenic modified vaccinia virus Ankara antigens L1 and A33	9
Development and preliminary validation of a MERS-CoV ELISA for serological testing of camels and alpacas	9
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India	9
Inactivation of infectious virus samples through UV irradiation for downstream analysis	9
The optimisation and application of a novel BaseScope™ RNA-ISH assay for the detection of foot-and-mouth disease virus in carrier African buffalo (Syncerus caffer) from South Africa	9
Evaluation of the impact of hollow fiber pore size and membrane material on virus concentration using a handheld hollow fiber method	9
Development and validation of an all-in-one rabies virus Bat-Clade genomic sequencing and host identification protocol	9
Effective plant virus enrichment using carbon nanotubes and microfluidics	9
Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays	9
High-dimensional droplet digital PCR of multiple hepatitis B splice variants in serum, liver, and tissue culture	9
Cryopreservation of chicken and duck tracheal rings and precision-cut lung slices: A promising tool for the rapid characterization of avian influenza viruses	9
Development of a sensitive real-time quantitative RT-PCR assay for the detection of pear chlorotic leaf spot-associated virus	9
Duplex droplet digital PCR enables simultaneous quantification of algal giant virus DSLLAV1 and virophage DSLV8 in natural and laboratory samples	8
Insights into antiviral activity of chlorpromazine against RNA viruses: Molecular docking, ADME profile, and semi-in vivo study	8
Optimizing virus inactivation methods for molecular detection techniques: Implications for viral protein and RNA measurements	8
Development of a CRISPR-Cas13a assay for mouse hepatitis virus detection	8
Protocol for virome characterization in low-volume respiratory samples from broiler chickens	8
Real time PCR-based evaluation of live attenuated lumpy skin disease virus vaccines for immunogenicity and efficacy	8
Establishment of a fetal cow (Bos Borus) skin fibroblasts cell line with immortalized characterization through human telomerase reverse transcriptase (hTERT) ectopic expression	8
The co-application of nectin-1 and PRV gD monoclonal antibodies: An effective approach to block pseudorabies virus invasion	8
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus	8

Evaluation of filter paper to transport oro/nasopharyngeal samples to detect SARS-CoV-2 by RT-qPCR	8
Prokaryotic expression of porcine deltacoronavirus S gene truncated segment and establishment of indirect ELISA detection method	8
Rapid detection of porcine encephalomyocarditis virus (EMCV) by isothermal reverse transcription recombinase polymerase amplification assays	8
Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses	8
Inactivation of airborne SARS-CoV-2 by thyme volatile oil vapor phase	8
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa	8
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV	7
Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification	7
Design, development, and validation of a strand-specific RT-qPCR assay to differentiate replicating versus nonreplicating Rabies virus	7
SARS-CoV-2-specific T cell responses: a comparative analysis between QuantiFERON SARS-CoV-2, T-SPOT.COVID, and an in-house Omicron ELISpot	7
Editorial Board	7
Comparison of sample pretreatments used to distinguish between infectious and non-infectious foodborne viruses by RT-qPCR	7
A thermal cycler-based, homogenization-free plant total nucleic acid extraction method for plant viruses and viroids assay	7
Development and validation of a DIVA ELISA for differentiating BEFV infected from vaccinated animals	7
Effect of time and temperature on the stability of HPV and cellular nucleic acid using simulated dry self-samples	7
Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes	7
AI-driven strategies for enhancing Mpox surveillance and response in Africa	7
Development of an accurate and rapid method for whole genome characterization of canine parvovirus	7
Potential use of high-resolution melting analyses for SARS-CoV-2 genomic surveillance	7
Efficiency of CMV serodiagnosis during pregnancy in daily laboratory routine	7
Editorial Board	7
A double-strain TM (gp45) polypeptide antigen and its application in the serodiagnosis of equine infectious anemia	7
Saliva samples in SARS-Cov-2 virus detection compared to the nasopharyngeal RT-PCR findings in individuals with suspected COVID-19 infection	7
Rapid metagenomic identification of two major swine pathogens with real-time nanopore sequencing	7
Detection of antibodies against infectious bronchitis virus strain QX (GI-19) by commercial ELISA kits and virus neutralization test	7
One-step TaqMan® RT-qPCR detection of sugar beet-infecting poleroviruses in Myzus persicae from yellow water pan traps opens up new possibilities for early risk assessment of virus yellows disease	7
Exploring the potential therapeutic effects of ultradiluted Crotalus horridus on antibody-dependent enhancement in dengue virus pathogenesis	7
Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection	7
Potential of recombinant avian adeno-associated virus as a viral vector for CRISPR/Cas9 delivery to avian cells	7
Rapid and sensitive SYBR Green-based RT-qPCR assays for the detection and quantification of mink coronavirus	7
A novel methodology for assessing contact tracing precision: Phylogenetic validation of a contact tracing program for COVID-19 in Belgium	7
Integrated validation of internally controlled one-step multiplex real-time RT-PCR for dengue virus serotyping and monoplex assay for quantification	6
A rapid immunochromatographic assay for the detection of BK Polyomavirus in urine samples from hematopoietic stem cell transplant recipients	6
Development of a one-step RT-qPCR assay for the detection of Grapevine leafroll-associated virus 7.	6
Characterization of monoclonal antibodies targeting SARS-CoV-2 spike glycoprotein: Reactivity against Delta and Omicron BA.1 variants	6
Simultaneous expression of three reporter proteins from a porcine reproductive and respiratory syndrome virus-based vector	6
Development and applications of a TaqMan based quantitative real-time PCR for the rapid detection of Pigeon circovirus (PiCV)	6
Product Enhanced Reverse Transcriptase for assessing replication competent virus in vectors retroviral vectors pseudotyped with GALV and VSV-G envelopes	6
Hybrid next-generation sequencing protocol for testing HIV-2 drug resistance	6
Transfer factor from REV-challenged chicken spleens enhances protection against reticuloendotheliosis virus infection in chicks	6
Antiviral effect of an extract from Kaempferia galanga L. rhizome in mice infected with pseudorabies virus	6
Fully automated dried blood spot sample handling and extraction for BoHV-1 antibody testing by ELISA	6
Rapid and efficient generation of viral genome knock-in cell lines using the CRISPR-Cas9 system to produce infectious virus	6
Simultaneous detection of five viruses and two viroids affecting apples through a DNA macroarray chip	6
Comparison of Polymerase Chain Reaction (PCR) assay performance in detecting Decapod penstylhamaparvovirus 1 in penaeid shrimp	6
A novel microarray-based PCR assay for the detection of HSV-1, HSV-2, and VZV skin infections: A retrospective analysis	6
Development of In Vitro assay for viral escape from broadly neutralizing antibodies	6
Single-domain antibodies applied as antiviral immunotherapeutics	6
Development of a novel multiplex digital PCR-based method for the detection of HTLV-1 proviral deletion	6
Evaluating the effectiveness of a novel environmental decontamination system utilizing low-energy hyper-charged photoelectrons against coronavirus	6
Development and validation of reverse-transcription cross-priming amplification-based lateral flow assay for the detection of infectious hematopoietic necrosis virus	6
MALDI-TOF nucleic acid mass spectrometry for simultaneously detection of fourteen porcine viruses and its application	6
A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine	6
m-PIMA™ HIV1/2 VL: A suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa	6
Detection of cell impurities and NF-κB activation using SEAP reporter cell lines in vaccines	5
Effective RNA extraction with readily available reagents: A comparative analysis of alternative protocols	5
Editorial Board	5
Development of a validated molecular analytical method to determine the viral safety of F(AB´)2 products: A novel application for a well-known technique	5
Comparative analysis of RT-qPCR assay sensitivity and process limit of detection for Japanese encephalitis virus (JEV) detection in piggery wastewater	5
Validation of an HPLC-CAD method for measuring the lipid content of novel LNP-encapsulated COVID-19 mRNA vaccines	5
Validation of laboratory developed serology assays for detection of IgG antibody to severe acute respiratory syndrome coronavirus 2 in the South African population	5
Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV)	5
Comparing methods to detect cellular proteins on the surface of HIV-1 virions	5
Critical parameters on Zika virus-like particles’ generation	5
Indirect ELISA based on truncated N protein for detecting Porcine deltacoronavirus-specific IgA antibodies	5
Establishment of a rapid real-time fluorescence-based recombinase-aided amplification method for detection of avian infectious bronchitis virus	5
Specific antibody production using recombinant proteins to elucidate seed transmission and nuclear localization of Coguvirus citrulli and Coguvirus henanense in radicles of watermelon crop	5
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid test for PPRV antibody	5
Editorial Board	5
‘CoviSwiftTM’: A point-of-care RT-PCR device for SARS-CoV-2 and its variant detection	5
Development of an indirect ELISA test for the detection of Tilapia lake virus (TiLV) in fish tissue and mucus samples	5
A new real-time RT-PCR for the detection of piscine orthoreoviruses PRV-1 and PRV-3	5
Performance evaluation of a SYBR Green-based real-time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples	5
Establishment and application of multiplex PCR for rapid detection of three mink diarrhea-associated viruses	5
Development of a one-step RT-qPCR assay for the detection of Citrus concave gum-associated virus in apples	5
Comparison of whole-viral genome sequencing and PCR-based assay for anal HPV detection to inform clinical implementation	5
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region	5
Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus	5
Development of an in-house Indirect ELISA for detection of bovine viral diarrhoea virus antibodies in bovine sera	5
Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples	5

Assessing the efficacy of different bead-based assays in capturing hepatitis E virus	5
Role of human cytomegalovirus in the pathogenesis of periapical lesions – A systematic review and meta-analysis	5
Preliminary screening reveals HPV-derived cDNA in the HNCF-PI 52 cell line originally derived from normal cervical tissue	5