Journal of Virological Methods

Article	Citations
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples	88
Implication of cell culture methods and biases on UV inactivation of viruses	51
Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction	47
Analysis of the antigenic and immunogenic properties of the native rabies virus glycoprotein purified by Lens culinaris lectin affinity chromatography	44
An indirect ELISA to detect antibodies to the gC of bovine alphaherpesvirus 1 (BoAHV1) displaying no crossreactivity with antibodies induced by bovine alphaherpesvirus 5 (BoAHV5)	43
Editorial Board	32
Clinical validation of the Roche cobas HPV test on the Roche cobas 6800 system for the purpose of cervical screening and qualification as a second-generation comparator test	31
The development of a rapid, high-throughput neutralization assay using a SARS-CoV-2 reporter	29
Reverse transcription recombinase polymerase amplification assay for rapid detection of the cucurbit chlorotic yellows virus	28
Rapid and sensitive detection of high pathogenicity Eurasian clade 2.3.4.4b avian influenza viruses in wild birds and poultry	25
Investigation of atypical serological profiles for Epstein-Barr virus (EBV)	25
Editorial Board	24
Development of an ARMS-Quadruplex-qPCR assay for the rapid identification of MPXV and the clades Ia, Ib, IIa and IIb	22
Editorial Board	22
WITHDRAWN: Development and evaluation of an indirect ELISA based on recombinant structural protein σC to detect antibodies against avian orthoreovirus	20
Detecting rubella virus-specific antibodies by cytopathic effect and immunoenzymatic microneutralization tests	19
Rapid identification of SARS-CoV-2 variants: Validation of the simplexa SARS-CoV-2 variant direct assay	19
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates	18
LSDV126 gene based molecular assays for specific detection and characterization of emerging Lumpy Skin Disease virus	17
Generation and application of immortalized sertoli cell line from sheep testis	17
Development and validation of a highly specific in-house chemiluminescent-based serological assay for the detection of antibodies directed against the human monkeypox virus	17
Detection of SARS-CoV-2 nucleocapsid antigen from serum can aid in timing of COVID-19 infection	16
Comparison of EBOV GP IgG antibody reactivity: Results from two immunoassays in the Democratic Republic of the Congo	16
A SYBR Green-based real-time RT-PCR assay to differentiate the H1N1 influenza virus lineages	16
Tween 80 improves the infectivity of BCL1 cell-adapted infectious bursal disease virus	16

Diagnosis of Monkeypox infection: Validation of two diagnostic kits for viral detection using RT-PCR	16
Discrepant results of hepatitis B virus genotype determination by PCR and DNA sequencing	15
The development of RT-RPA and CRISPR-Cas12a based assay for sensitive detection of infectious hematopoietic necrosis virus (IHNV)	15
A systematic review on current approaches in bat virus discovered between 2018 and 2022	15
Performance evaluation of TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV) for detection and differentiation of dengue and chikungunya viral RNA in serum samples of symptomatic patients	15
A viral metagenomic protocol for nanopore sequencing of group A rotavirus	15
A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator	14
Double testing with lateral flow antigen test devices for COVID-19: does a second test in quick succession add value?	14
Relation of vitamin D to COVID-19	13
Developing Zika virus-transduced hACE2 expression models for severe acute respiratory syndrome coronavirus 2 infection in vitro and in vivo	13
An optimised protocol for the expression and purification of adenovirus core protein VII	13
Flagellin and elongation factor of Bacillus velezensis (VB7) reprogramme the immune response in tomato towards the management of GBNV infection	13
Efficient virus-induced gene silencing system in pumpkin (Cucurbita maxima) using apple latent spherical virus vector	13
Quantitative real time PCR detection of Saboya virus: A flavivirus member of yellow fever genetic group	13
Purification and characterization of kyasanur forest disease virus EDIII domain of major envelope glycoprotein	13
Evaluation of inactivated snakehead rhabdovirus as an internal positive control for RT-qPCR diagnosis of viral hemorrhagic septicemia virus in fish	13
Investigation of variants in genetics and virulence of Porcine Epidemic Diarrhea Virus after serial passage on Vero cells	13
Development of a protocol for the elimination of Cyrtanthus elatus virus-A from Narcissus tazetta by in vitro chemotherapy in combination with electrotherapy	13
A multi-center study comparing the analytical sensitivity and clinical concordance of three HIV-1 viral load assays	13
Concordance in RT-PCR detection of SARS-CoV-2 between samples preserved in viral and bacterial transport medium	13
Improvement of SARS-COV-2 screening using pooled sampling testing in limited RT-qPCR resources	13
Nucleotide amplification and sequencing of the GC-rich region between matrix and fusion protein genes of peste des petits ruminants virus	13
Subtype specific virus enrichment with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus	13
Use of the Ct difference between the Nucleocapside (N) and the Spike (S) or RNA-dependent RNA polymerase (RdRP) genes as a preliminary screening for SARS-CoV-2 variants with the Allplex™ SARS-CoV-2/Fl	13
Agrobacterium-mediated co-inoculation of okra plants with cloned okra enation leaf curl virus DNA and bhendi yellow vein mosaic beta-satellite DNA furthers Koch’s postulates for enation leaf curl dise	13
Editorial Board	12
Development and validation of an all-in-one rabies virus Bat-Clade genomic sequencing and host identification protocol	12
Comparison of the PowerChek SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit and BioFire Respiratory Panel 2.1 for simultaneous detection of SARS-CoV-2, influenza A and B, and respirator	12
Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring	12
Evaluation of the flinders technology associates cards for storage and molecular detection of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) DNA	12
A novel high-throughput sequencing approach reveals the presence of a new virus infecting Rosa: rosa ilarvirus-1 (RIV-1)	12
Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin	12
Disassembly mediated multimodal chromatography based purification of HPV-VLPs produced in Pichia pastoris	12
IC-ELISAs for the quantitative detection of monkeypox virus cross-immunogenic modified vaccinia virus Ankara antigens L1 and A33	12
Evaluation of a concentration method for the recovery of human adenovirus from mineral water, tap water and well water	12
Adjusting susceptibilities of C57BL/6 mice to orthoflaviviruses for evaluation of antiviral drugs by altering the levels of interferon alpha/beta receptor function	11
Effective plant virus enrichment using carbon nanotubes and microfluidics	11
Development and preliminary validation of a MERS-CoV ELISA for serological testing of camels and alpacas	11
Phi 6 recovery from inoculated fingerpads based on elution buffer and methodology	11
Evaluation of the impact of hollow fiber pore size and membrane material on virus concentration using a handheld hollow fiber method	11
Evaluation of filter paper to transport oro/nasopharyngeal samples to detect SARS-CoV-2 by RT-qPCR	11
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa	10
Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine	10
Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (RPA-LFA)	10
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India	10
Development of a sensitive real-time quantitative RT-PCR assay for the detection of pear chlorotic leaf spot-associated virus	10
The optimisation and application of a novel BaseScope™ RNA-ISH assay for the detection of foot-and-mouth disease virus in carrier African buffalo (Syncerus caffer) from South Africa	10
Inactivation of airborne SARS-CoV-2 by thyme volatile oil vapor phase	10
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus	10
A low-cost simple test for weekly detection of Mycoplasma hyorhinis and arginini contaminations in cell cultures and viral preparations	10

Validation of the analytical performance of nine commercial RT-qPCR kits for SARS-CoV-2 detection using certified reference material	10
Prokaryotic expression of porcine deltacoronavirus S gene truncated segment and establishment of indirect ELISA detection method	10
Chemical inactivation of foot-and-mouth disease virus in bovine tongue epithelium for safe transport and downstream processing	10
Preparation and evaluation of IgY against human papillomavirus	10
Protocol for virome characterization in low-volume respiratory samples from broiler chickens	10
Reply to the letter from Dr. Nikolayevskyy et al. referring to our publication published in the Journal of Virological Methods 298 (2021)11:4295 “Evaluation of the QuantiFERON SARS-CoV-2 Interferon-ɣ	10
High-dimensional droplet digital PCR of multiple hepatitis B splice variants in serum, liver, and tissue culture	10
Optimizing virus inactivation methods for molecular detection techniques: Implications for viral protein and RNA measurements	10
Evaluation of sample pooling using the SAMBA II SARS-CoV-2 test	9
One-step TaqMan® RT-qPCR detection of sugar beet-infecting poleroviruses in Myzus persicae from yellow water pan traps opens up new possibilities for early risk assessment of virus yellows disease	9
Rapid metagenomic identification of two major swine pathogens with real-time nanopore sequencing	9
Rapid detection of porcine encephalomyocarditis virus (EMCV) by isothermal reverse transcription recombinase polymerase amplification assays	9
Design, development, and validation of a strand-specific RT-qPCR assay to differentiate replicating versus nonreplicating Rabies virus	9
Detection of antibodies against infectious bronchitis virus strain QX (GI-19) by commercial ELISA kits and virus neutralization test	9
A multiplex RT-PCR assay combined with co-extraction of DNA and RNA for simultaneous detection of TYLCV and ToCV in whitefly	9
Potential use of high-resolution melting analyses for SARS-CoV-2 genomic surveillance	9
A double-strain TM (gp45) polypeptide antigen and its application in the serodiagnosis of equine infectious anemia	9
Development of a reverse-transcription loop-mediated isothermal amplification assay for the detection of Tobacco mild green mosaic virus (TMGMV)	9
Effect of time and temperature on the stability of HPV and cellular nucleic acid using simulated dry self-samples	9
Development of an accurate and rapid method for whole genome characterization of canine parvovirus	9
Rapid diagnostic detection of tomato apical stunt viroid based on isothermal reverse transcription-recombinase polymerase amplification	9
Editorial Board	9
Characterization and use in neutralization assays of avian influenza codon-optimized H5 and H7 retroviral pseudotypes	9
SARS-CoV-2-specific T cell responses: a comparative analysis between QuantiFERON SARS-CoV-2, T-SPOT.COVID, and an in-house Omicron ELISpot	9
Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses	9
Establishment of a fetal cow (Bos Borus) skin fibroblasts cell line with immortalized characterization through human telomerase reverse transcriptase (hTERT) ectopic expression	9
Diagnostic techniques for COVID-19: A mini-review	9
Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material	9
Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes	9
Development and validation of a DIVA ELISA for differentiating BEFV infected from vaccinated animals	9
A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus	8
Development and applications of a TaqMan based quantitative real-time PCR for the rapid detection of Pigeon circovirus (PiCV)	8
Development of a one-step RT-qPCR assay for the detection of Grapevine leafroll-associated virus 7.	8
Editorial Board	8
Evaluation of sensitivity and specificity in RNA-Seq-based detection of grapevine viral pathogens	8
Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1)	8
A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine	8
A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the BVDV and BPIV3	8
Simultaneous detection of five viruses and two viroids affecting apples through a DNA macroarray chip	8
Rapid and efficient generation of viral genome knock-in cell lines using the CRISPR-Cas9 system to produce infectious virus	8
An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing	8
Fully automated dried blood spot sample handling and extraction for BoHV-1 antibody testing by ELISA	8
Efficiency of CMV serodiagnosis during pregnancy in daily laboratory routine	8
Comparison of sample pretreatments used to distinguish between infectious and non-infectious foodborne viruses by RT-qPCR	8
Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification	8
Simultaneous expression of three reporter proteins from a porcine reproductive and respiratory syndrome virus-based vector	8
A thermal cycler-based, homogenization-free plant total nucleic acid extraction method for plant viruses and viroids assay	8
Antiviral effect of an extract from Kaempferia galanga L. rhizome in mice infected with pseudorabies virus	8
Characterization of monoclonal antibodies targeting SARS-CoV-2 spike glycoprotein: Reactivity against Delta and Omicron BA.1 variants	8
Detection of Sri Lankan cassava mosaic virus by loop-mediated isothermal amplification using dried reagents	8
Comparison of in-house SARS-CoV-2 genome extraction procedures. A need for COVID-19 pandemic	8
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV	8
Development of a novel multiplex digital PCR-based method for the detection of HTLV-1 proviral deletion	8
Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection	8
Concordance of Anyplex™ II HPV HR assays with reference HPV assays in cervical cancer screening: Systematic review	8
Detection and differentiation of murine leukemia virus (MLV) and murine stem cell virus (MSCV) and therefrom derived nucleic acids	7
Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum	7
Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics	7
Comparing methods to detect cellular proteins on the surface of HIV-1 virions	7
Preliminary establishment and validation of a loop-mediated isothermal amplification assay for convenient screening of 13 types of high-risk human papillomaviruses in cervical secretions	7
Development of an in-house Indirect ELISA for detection of bovine viral diarrhoea virus antibodies in bovine sera	7
Product Enhanced Reverse Transcriptase for assessing replication competent virus in vectors retroviral vectors pseudotyped with GALV and VSV-G envelopes	7
MALDI-TOF nucleic acid mass spectrometry for simultaneously detection of fourteen porcine viruses and its application	7
Performance evaluation of automated chemiluminescence immunoassay based antigen detection – Moving towards more reliable ways to predict SARS-CoV-2 infection	7
Assessing the efficacy of different bead-based assays in capturing hepatitis E virus	7
Development and evaluation of a ligation-free sequence-independent, single-primer amplification (LF-SISPA) assay for whole genome characterization of viruses	7
Development and validation of novel kit for quantification of SARS-CoV-2 antibodies on clinical samples	7
‘CoviSwiftTM’: A point-of-care RT-PCR device for SARS-CoV-2 and its variant detection	7
Hybrid next-generation sequencing protocol for testing HIV-2 drug resistance	7
Development and validation of reverse-transcription cross-priming amplification-based lateral flow assay for the detection of infectious hematopoietic necrosis virus	7
A multiplex nanoparticle-assisted polymerase chain reaction assay for detecting three canine epidemic viruses using a dual priming oligonucleotide system	7
Rapid detection of Potato leafroll virus and Potato virus Y by reverse transcription loop-mediated isothermal amplification method in north-east India	7
A novel microarray-based PCR assay for the detection of HSV-1, HSV-2, and VZV skin infections: A retrospective analysis	7
m-PIMA™ HIV1/2 VL: A suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa	7
SARS-CoV-2 multiplex RT-PCR to detect variants of concern (VOCs) in Malaysia, between January to May 2021	7
Validation of an HPLC-CAD method for measuring the lipid content of novel LNP-encapsulated COVID-19 mRNA vaccines	7
Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples	7
Comparison of Polymerase Chain Reaction (PCR) assay performance in detecting Decapod penstylhamaparvovirus 1 in penaeid shrimp	7
Development of an indirect ELISA test for the detection of Tilapia lake virus (TiLV) in fish tissue and mucus samples	6
A validated in-house assay for HIV drug resistance mutation surveillance from dried blood spot specimens	6

Specific antibody production using recombinant proteins to elucidate seed transmission and nuclear localization of Coguvirus citrulli and Coguvirus henanense in radicles of watermelon crop	6
Critical parameters on Zika virus-like particles’ generation	6
Detection of a novel porcine circovirus 4 in Korean pig herds using a loop-mediated isothermal amplification assay	6
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid test for PPRV antibody	6
Monoclonal antibody based solid phase competition ELISA to detect FMDV serotype A specific antibodies	6
nanoString evaluation of murine Cytomegalovirus transcription in vivo and in vitro	6
Editorial Board	6
Indirect ELISA for analysis of malignant catarrhal fever virus-specific antibodies in a range of species	6
Editorial Board	6
Single-domain antibodies applied as antiviral immunotherapeutics	6
The Coris BioConcept COVID 19 Ag Respi-Strip, a field experience feedback	6
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region	6
Next-generation sequencing of human respiratory syncytial virus subgroups A and B genomes	6
Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening	6
Effective RNA extraction with readily available reagents: A comparative analysis of alternative protocols	6
Editorial Board	6
Development of a one-step RT-qPCR assay for the detection of Citrus concave gum-associated virus in apples	6
Development of a validated molecular analytical method to determine the viral safety of F(AB´)2 products: A novel application for a well-known technique	6
Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages	6
Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens	6
Production and application of monoclonal antibodies against ORF66 of cyprinid herpesvirus 2	6
Establishment of a rapid real-time fluorescence-based recombinase-aided amplification method for detection of avian infectious bronchitis virus	6
Development of two competitive ELISAs based on monoclonal antibodies for the serological detection of Bovine ephemeral fever virus	6
Magnetic Multiplex Loop Mediated Isothermal Amplification (MM-LAMP) technique for simultaneous detection of dengue and chikungunya virus	6
Validation of laboratory developed serology assays for detection of IgG antibody to severe acute respiratory syndrome coronavirus 2 in the South African population	6
Development of a one-step RT-qPCR detection assay for the newly described citrus viroid VII	6
Development of one step colorimetric RT-LAMP assays for rapid detection of Apple mosaic virus and Prunus necrotic ringspot virus	6
Establishment and application of multiplex PCR for rapid detection of three mink diarrhea-associated viruses	6
Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing	6
A hydroxypropyl methylcellulose plaque assay for human respiratory syncytial virus	6