Journal of Virological Methods

Article	Citations
Investigating the sequence variation in the influenza A matrix genes during the 2017-2018 and 2018-2019 seasons in samples from a local population in London	93
Editorial Board	82
Editorial Board	78
Investigation of atypical serological profiles for Epstein-Barr virus (EBV)	45
Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA	43
Implication of cell culture methods and biases on UV inactivation of viruses	42
May viral load detected in saliva in the early stages of infection be a prognostic indicator in COVID-19 patients?	42
A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions	32
Analysis of the antigenic and immunogenic properties of the native rabies virus glycoprotein purified by Lens culinaris lectin affinity chromatography	32
The development of a rapid, high-throughput neutralization assay using a SARS-CoV-2 reporter	31
An indirect ELISA to detect antibodies to the gC of bovine alphaherpesvirus 1 (BoAHV1) displaying no crossreactivity with antibodies induced by bovine alphaherpesvirus 5 (BoAHV5)	30
Rapid and sensitive detection of high pathogenicity Eurasian clade 2.3.4.4b avian influenza viruses in wild birds and poultry	30
Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction	28
Reverse transcription recombinase polymerase amplification assay for rapid detection of the cucurbit chlorotic yellows virus	26
Development and validation of a duplex RT-qPCR assay for norovirus quantification in wastewater samples	24
Tween 80 improves the infectivity of BCL1 cell-adapted infectious bursal disease virus	23
Diagnosis of Monkeypox infection: Validation of two diagnostic kits for viral detection using RT-PCR	23
Development of an ARMS-Quadruplex-qPCR assay for the rapid identification of MPXV and the clades Ia, Ib, IIa and IIb	23
Generation and application of immortalized sertoli cell line from sheep testis	23
A viral metagenomic protocol for nanopore sequencing of group A rotavirus	22
Rapid identification of SARS-CoV-2 variants: Validation of the simplexa SARS-CoV-2 variant direct assay	22
WITHDRAWN: Development and evaluation of an indirect ELISA based on recombinant structural protein σC to detect antibodies against avian orthoreovirus	21
Editorial Board	21
An integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) method to simultaneously quantify the infectious concentrations of eight environmentally relevant enterovirus serotypes	20
A SYBR Green-based real-time RT-PCR assay to differentiate the H1N1 influenza virus lineages	20

Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates	19
Performance of the Roche IL-6 chemiluminescent immunoassay in patients with COVID-like respiratory symptoms	19
Performance evaluation of TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV) for detection and differentiation of dengue and chikungunya viral RNA in serum samples of symptomatic patients	18
Development and validation of a highly specific in-house chemiluminescent-based serological assay for the detection of antibodies directed against the human monkeypox virus	18
A systematic review on current approaches in bat virus discovered between 2018 and 2022	18
Detection of SARS-CoV-2 nucleocapsid antigen from serum can aid in timing of COVID-19 infection	18
The development of RT-RPA and CRISPR-Cas12a based assay for sensitive detection of infectious hematopoietic necrosis virus (IHNV)	18
LSDV126 gene based molecular assays for specific detection and characterization of emerging Lumpy Skin Disease virus	18
Editorial Board	18
Discrepant results of hepatitis B virus genotype determination by PCR and DNA sequencing	18
Double testing with lateral flow antigen test devices for COVID-19: does a second test in quick succession add value?	17
Agrobacterium-mediated co-inoculation of okra plants with cloned okra enation leaf curl virus DNA and bhendi yellow vein mosaic beta-satellite DNA furthers Koch’s postulates for enation leaf curl dise	17
Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR	17
Nucleotide amplification and sequencing of the GC-rich region between matrix and fusion protein genes of peste des petits ruminants virus	17
Quantitative real time PCR detection of Saboya virus: A flavivirus member of yellow fever genetic group	16
Flagellin and elongation factor of Bacillus velezensis (VB7) reprogramme the immune response in tomato towards the management of GBNV infection	16
Development of a TaqMan-based real-time assay for the specific detection of canine astrovirus	16
An optimised protocol for the expression and purification of adenovirus core protein VII	16
Evaluation of inactivated snakehead rhabdovirus as an internal positive control for RT-qPCR diagnosis of viral hemorrhagic septicemia virus in fish	15
A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator	15
Relation of vitamin D to COVID-19	15
Concordance in RT-PCR detection of SARS-CoV-2 between samples preserved in viral and bacterial transport medium	15
Improvement of SARS-COV-2 screening using pooled sampling testing in limited RT-qPCR resources	15
Purification and characterization of kyasanur forest disease virus EDIII domain of major envelope glycoprotein	15
Use of the Ct difference between the Nucleocapside (N) and the Spike (S) or RNA-dependent RNA polymerase (RdRP) genes as a preliminary screening for SARS-CoV-2 variants with the Allplex™ SARS-CoV-2/Fl	14
A luciferase immunosorbent assay for quantitative detection of IgG antibodies against SARS-CoV-2 nucleoprotein	14
Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring	14
Analytical sensitivity comparison of 14 conventional and three rapid RT-PCR assays for SARS-CoV-2 detection	14
Editorial Board	14
Subtype specific virus enrichment with immunomagnetic separation method followed by NGS unravels the mixture of H5 and H9 avian influenza virus	14
The theory and practice of the viral dose in neutralization assay: Insights on SARS-CoV-2 “doublethink” effect	13
Development of a protocol for the elimination of Cyrtanthus elatus virus-A from Narcissus tazetta by in vitro chemotherapy in combination with electrotherapy	13
A novel high-throughput sequencing approach reveals the presence of a new virus infecting Rosa: rosa ilarvirus-1 (RIV-1)	13
Efficient virus-induced gene silencing system in pumpkin (Cucurbita maxima) using apple latent spherical virus vector	13
Investigation of variants in genetics and virulence of Porcine Epidemic Diarrhea Virus after serial passage on Vero cells	13
A multi-center study comparing the analytical sensitivity and clinical concordance of three HIV-1 viral load assays	13
Surface plasmon resonance approach to study drug interactions with SARS-CoV-2 RNA-dependent RNA polymerase highlights treatment potential of suramin	12
Development of immunochromatographic strip assay for rapid detection of novel goose astrovirus	12
Reply to the letter from Dr. Nikolayevskyy et al. referring to our publication published in the Journal of Virological Methods 298 (2021)11:4295 “Evaluation of the QuantiFERON SARS-CoV-2 Interferon-ɣ	12
Development and validation of an all-in-one rabies virus Bat-Clade genomic sequencing and host identification protocol	12
Simultaneous detection and differentiation of porcine circovirus 3 and 4 using a SYBR Green І-based duplex quantitative PCR assay	12
Development of real-time RT-qPCR assays for the typing of two novel bluetongue virus genotypes derived from sheeppox vaccine	12
Comparison of the PowerChek SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit and BioFire Respiratory Panel 2.1 for simultaneous detection of SARS-CoV-2, influenza A and B, and respirator	12
Inactivation of airborne SARS-CoV-2 by thyme volatile oil vapor phase	12
Multiplex PCR method for MinION sequencing of Bagaza virus isolated from wild caught mosquitoes in South Africa	11
Development of a sensitive real-time quantitative RT-PCR assay for the detection of pear chlorotic leaf spot-associated virus	11
A low-cost simple test for weekly detection of Mycoplasma hyorhinis and arginini contaminations in cell cultures and viral preparations	11
Establishment of a fetal cow (Bos Borus) skin fibroblasts cell line with immortalized characterization through human telomerase reverse transcriptase (hTERT) ectopic expression	11
Evaluation of filter paper to transport oro/nasopharyngeal samples to detect SARS-CoV-2 by RT-qPCR	11
A reverse transcription-multiplex PCR strategy devised for concomitant detection and differentiation of foot and mouth disease virus serotypes O, A and Asia 1 in India	11

Adjusting susceptibilities of C57BL/6 mice to orthoflaviviruses for evaluation of antiviral drugs by altering the levels of interferon alpha/beta receptor function	11
Effective plant virus enrichment using carbon nanotubes and microfluidics	11
Phi 6 recovery from inoculated fingerpads based on elution buffer and methodology	11
Development of an accurate and rapid method for whole genome characterization of canine parvovirus	11
Reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of duck Tembusu virus	11
Rapid onsite detection of piper yellow mottle virus infecting black pepper by recombinase polymerase amplification-lateral flow assay (RPA-LFA)	11
Prokaryotic expression of porcine deltacoronavirus S gene truncated segment and establishment of indirect ELISA detection method	11
Preparation and evaluation of IgY against human papillomavirus	11
Corrigendum to “Development of a one-step RT-PCR assay for detection of pancoronaviruses (α-, β-, γ-, and δ-coronaviruses) using newly designed degenerate primers for porcine and avian `fecal samples”	11
Evaluation of the impact of hollow fiber pore size and membrane material on virus concentration using a handheld hollow fiber method	11
Validation of the analytical performance of nine commercial RT-qPCR kits for SARS-CoV-2 detection using certified reference material	11
Development and preliminary validation of a MERS-CoV ELISA for serological testing of camels and alpacas	11
Rapid metagenomic identification of two major swine pathogens with real-time nanopore sequencing	10
Editorial Board	10
Development of a reverse-transcription loop-mediated isothermal amplification assay for the detection of Tobacco mild green mosaic virus (TMGMV)	10
Rapid diagnostic detection of tomato apical stunt viroid based on isothermal reverse transcription-recombinase polymerase amplification	10
Diagnostic techniques for COVID-19: A mini-review	10
Rapid detection of porcine encephalomyocarditis virus (EMCV) by isothermal reverse transcription recombinase polymerase amplification assays	10
Improvement in the sensitivity of viroid detection by adapting the reverse transcription step in one-step RT-qPCR assays	10
Optimizing virus inactivation methods for molecular detection techniques: Implications for viral protein and RNA measurements	10
Temperature-dependent secretion of Zika virus envelope and non-structural protein 1 in mammalian cells for clinical applications	10
Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements	10
Newly-designed primer pairs for the detection of type 2 porcine reproductive and respiratory syndrome virus genes	10
Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material	10
Multiplexed, microscale, microarray-based serological assay for antibodies against all human-relevant coronaviruses	10
Development and evaluation of a multiplex real-time RT-PCR assay for simultaneous detection of H5, H7, and H9 subtype avian influenza viruses	10
Chemical inactivation of foot-and-mouth disease virus in bovine tongue epithelium for safe transport and downstream processing	10
Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification	9
Editorial Board	9
Development of a double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of VZV	9
SARS-CoV-2-specific T cell responses: a comparative analysis between QuantiFERON SARS-CoV-2, T-SPOT.COVID, and an in-house Omicron ELISpot	9
Design, development, and validation of a strand-specific RT-qPCR assay to differentiate replicating versus nonreplicating Rabies virus	9
Comparison of Polymerase Chain Reaction (PCR) assay performance in detecting Decapod penstylhamaparvovirus 1 in penaeid shrimp	9
Development and applications of a TaqMan based quantitative real-time PCR for the rapid detection of Pigeon circovirus (PiCV)	9
Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1)	9
Efficiency of CMV serodiagnosis during pregnancy in daily laboratory routine	9
Effect of time and temperature on the stability of HPV and cellular nucleic acid using simulated dry self-samples	9
Evaluation of sample pooling using the SAMBA II SARS-CoV-2 test	9
Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay	9
Potential use of high-resolution melting analyses for SARS-CoV-2 genomic surveillance	9
Label-free imaging of nuclear membrane for analysis of nuclear import of viral complexes	9
Performance evaluation of automated chemiluminescence immunoassay based antigen detection – Moving towards more reliable ways to predict SARS-CoV-2 infection	9
A thermal cycler-based, homogenization-free plant total nucleic acid extraction method for plant viruses and viroids assay	9
Concordance of Anyplex™ II HPV HR assays with reference HPV assays in cervical cancer screening: Systematic review	9
Rapid titration of recombinant baculoviruses based on NanoLuc secretion in early infection	9
Comparison of in-house SARS-CoV-2 genome extraction procedures. A need for COVID-19 pandemic	9
Characterization and use in neutralization assays of avian influenza codon-optimized H5 and H7 retroviral pseudotypes	9
A luciferase-based approach for measuring HBGA blockade antibody titers against human norovirus	9
A multiplex RT-PCR assay combined with co-extraction of DNA and RNA for simultaneous detection of TYLCV and ToCV in whitefly	9
One-step TaqMan® RT-qPCR detection of sugar beet-infecting poleroviruses in Myzus persicae from yellow water pan traps opens up new possibilities for early risk assessment of virus yellows disease	9
Evaluation of sensitivity and specificity in RNA-Seq-based detection of grapevine viral pathogens	9
Fully automated dried blood spot sample handling and extraction for BoHV-1 antibody testing by ELISA	9
A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus	9
A double-strain TM (gp45) polypeptide antigen and its application in the serodiagnosis of equine infectious anemia	9
Development and validation of a DIVA ELISA for differentiating BEFV infected from vaccinated animals	9
An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing	8
Generation and characterization of luciferase-secreting, single-round infectious DENV-2 reporter for functional antibody assays	8
A new highly sensitive single-tube nested real-time PCR assay: Clinical utility in perinatal HIV-1 diagnosis	8
Detection of Sri Lankan cassava mosaic virus by loop-mediated isothermal amplification using dried reagents	8
Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum	8
Validation of an HPLC-CAD method for measuring the lipid content of novel LNP-encapsulated COVID-19 mRNA vaccines	8
Development of an in-house Indirect ELISA for detection of bovine viral diarrhoea virus antibodies in bovine sera	8
Antiviral effect of an extract from Kaempferia galanga L. rhizome in mice infected with pseudorabies virus	8
Development of a novel multiplex digital PCR-based method for the detection of HTLV-1 proviral deletion	8
Simultaneous expression of three reporter proteins from a porcine reproductive and respiratory syndrome virus-based vector	8
MALDI-TOF nucleic acid mass spectrometry for simultaneously detection of fourteen porcine viruses and its application	8
Development and validation of reverse-transcription cross-priming amplification-based lateral flow assay for the detection of infectious hematopoietic necrosis virus	8
Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics	8
Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples	8
A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine	8
Characterization of monoclonal antibodies targeting SARS-CoV-2 spike glycoprotein: Reactivity against Delta and Omicron BA.1 variants	8
Development of a one-step RT-qPCR assay for the detection of Grapevine leafroll-associated virus 7.	8
A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the BVDV and BPIV3	8
Simultaneous detection of five viruses and two viroids affecting apples through a DNA macroarray chip	8
A multiplex nanoparticle-assisted polymerase chain reaction assay for detecting three canine epidemic viruses using a dual priming oligonucleotide system	8
SARS-CoV-2 multiplex RT-PCR to detect variants of concern (VOCs) in Malaysia, between January to May 2021	8
Proteinase K treatment in absence of RNA isolation classical procedures is a quick and cheaper alternative for SARS-CoV-2 molecular detection	8
A novel microarray-based PCR assay for the detection of HSV-1, HSV-2, and VZV skin infections: A retrospective analysis	7
Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus	7
Development and validation of novel kit for quantification of SARS-CoV-2 antibodies on clinical samples	7

Editorial Board	7
Detection and differentiation of murine leukemia virus (MLV) and murine stem cell virus (MSCV) and therefrom derived nucleic acids	7
Inactivation of SARS-CoV-2 isolates from lineages B.1.1.7 (Alpha), P.1 (Gamma) and B.1.110 by heating and UV irradiation	7
Assessing the efficacy of different bead-based assays in capturing hepatitis E virus	7
Analysis of external quality assessment samples revealed crucial performance differences between commercial RT-PCR assays for SARS-CoV-2 detection when taking extraction methods and real-time-PCR inst	7
Preliminary establishment and validation of a loop-mediated isothermal amplification assay for convenient screening of 13 types of high-risk human papillomaviruses in cervical secretions	7
The Coris BioConcept COVID 19 Ag Respi-Strip, a field experience feedback	7
‘CoviSwiftTM’: A point-of-care RT-PCR device for SARS-CoV-2 and its variant detection	7
Product Enhanced Reverse Transcriptase for assessing replication competent virus in vectors retroviral vectors pseudotyped with GALV and VSV-G envelopes	7
m-PIMA™ HIV1/2 VL: A suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa	7
Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens	7
Comparing methods to detect cellular proteins on the surface of HIV-1 virions	7
Development of an indirect ELISA test for the detection of Tilapia lake virus (TiLV) in fish tissue and mucus samples	7
Editorial Board	7
Development and evaluation of a ligation-free sequence-independent, single-primer amplification (LF-SISPA) assay for whole genome characterization of viruses	7
Single-domain antibodies applied as antiviral immunotherapeutics	7
Production and application of monoclonal antibodies against ORF66 of cyprinid herpesvirus 2	7
Rapid detection of Potato leafroll virus and Potato virus Y by reverse transcription loop-mediated isothermal amplification method in north-east India	7
Hybrid next-generation sequencing protocol for testing HIV-2 drug resistance	7
Development of a validated molecular analytical method to determine the viral safety of F(AB´)2 products: A novel application for a well-known technique	6
Development of a one-step RT-qPCR detection assay for the newly described citrus viroid VII	6
Development of two competitive ELISAs based on monoclonal antibodies for the serological detection of Bovine ephemeral fever virus	6
Two immune-based methods using immortalized porcine kidney macrophages for quantifying neutralizing activity against porcine reproductive and respiratory syndrome virus-2	6
A study of performance evaluation of cobas e 801 infectious diseases panel	6
In planta expression of specific single chain fragment antibody (scFv) against nucleocapsid protein of fig mosaic virus (FMV)	6
Development of a competitive ELISA method based on VLPs detecting the antibodies of serotype A FMDV	6
Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening	6
Editorial Board	6
A validated in-house assay for HIV drug resistance mutation surveillance from dried blood spot specimens	6
Critical parameters on Zika virus-like particles’ generation	6
Development of one step colorimetric RT-LAMP assays for rapid detection of Apple mosaic virus and Prunus necrotic ringspot virus	6
Monoclonal antibody based solid phase competition ELISA to detect FMDV serotype A specific antibodies	6
Development and validation of the first HBV qRT-PCR assay in the Mediterranean area targeting the X region	6
Comparison of serological techniques for the diagnosis of equine infectious Anemia in an endemic area of Argentina	6
nanoString evaluation of murine Cytomegalovirus transcription in vivo and in vitro	6
Editorial Board	6
Comparison of the AmpFire and MA-6000 polymerase chain reaction platforms for high-risk human papillomavirus testing in cervical precancer screening	6
Spectrophotometric microplate assay for titration and neutralization of avian nephritis virus based on the virus cytopathicity	6
Improved SARS-CoV-2 RNA recovery in wastewater matrices using a CTAB-based extraction method	6
Magnetic Multiplex Loop Mediated Isothermal Amplification (MM-LAMP) technique for simultaneous detection of dengue and chikungunya virus	6
A hydroxypropyl methylcellulose plaque assay for human respiratory syncytial virus	6
Phenotypic characterization of cell culture-derived hepatitis E virus subjected to different chemical treatments: Application in virus removal via nanofiltration	6
Indirect ELISA for analysis of malignant catarrhal fever virus-specific antibodies in a range of species	6
Combined nasal- and oropharyngeal self-swab provides equivalent performance compared to professionally collected oropharyngeal swabs in detecting SARS-CoV-2 in a real-life setting	6
Establishment and application of multiplex PCR for rapid detection of three mink diarrhea-associated viruses	6
Genome sequencing of the mpox virus 2022 outbreak with amplicon-based Oxford Nanopore MinION sequencing	6
Editorial Board	6
Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages	6
Validation of laboratory developed serology assays for detection of IgG antibody to severe acute respiratory syndrome coronavirus 2 in the South African population	6
Quantitative serum HBV markers in predicting phases of natural history of chronic HBV infection	6
Evaluation of PCR-based hemagglutinin subtyping as a tool to aid in surveillance of avian influenza viruses in migratory wild birds	6
Next-generation sequencing of human respiratory syncytial virus subgroups A and B genomes	6
Establishment of a rapid real-time fluorescence-based recombinase-aided amplification method for detection of avian infectious bronchitis virus	6
Detection of a novel porcine circovirus 4 in Korean pig herds using a loop-mediated isothermal amplification assay	6
Specific antibody production using recombinant proteins to elucidate seed transmission and nuclear localization of Coguvirus citrulli and Coguvirus henanense in radicles of watermelon crop	6
Development of a sensitive immunochromatographic method using lanthanide fluorescent microsphere for rapid test for PPRV antibody	6
Quantification of intrahepatic cccDNA in HBV associated hepatocellular carcinoma by improved ddPCR method	6