Journal of Virological Methods

Article	Citations
Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit	142
Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay	95
Performance evaluation of the Roche Elecsys Anti-SARS-CoV-2 S immunoassay	86
Comparison of potency assays to assess SARS-CoV-2 neutralizing antibody capacity in COVID-19 convalescent plasma	76
The screening value of RT-LAMP and RT-PCR in the diagnosis of COVID-19: systematic review and meta-analysis	72
Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater	67
SARS-CoV-2: Comparative analysis of different RNA extraction methods	57
A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs	57
The effect of heat-treatment on SARS-CoV-2 viability and detection	57
Genomic and evolutionary comparison between SARS-CoV-2 and other human coronaviruses	47
Evaluation of the QuantiFERON SARS-CoV-2 interferon-ɣ release assay in mRNA-1273 vaccinated health care workers	40
Time series analysis and predicting COVID-19 affected patients by ARIMA model using machine learning	40
Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19	39
Comparison analysis of different swabs and transport mediums suitable for SARS-CoV-2 testing following shortages	38
Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2	37
Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2	36
Establishment of an SYBR Green-based real-time PCR assay for porcine circovirus type 4 detection	34
Madin-Darby bovine kidney (MDBK) cells are a suitable cell line for the propagation and study of the bovine poxvirus lumpy skin disease virus	32
Reliable detection of SARS-CoV-2 with patient-collected swabs and saline gargles: A three-headed comparison on multiple molecular platforms	32
Comparison of two commercial surrogate ELISAs to detect a neutralising antibody response to SARS-CoV-2	31
Sample pooling strategies for SARS-CoV-2 detection	30
Improving human coronavirus OC43 (HCoV-OC43) research comparability in studies using HCoV-OC43 as a surrogate for SARS-CoV-2	30
Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda	30
Rapid and sensitive detection of high pathogenicity Eurasian clade 2.3.4.4b avian influenza viruses in wild birds and poultry	29
Development a multiplex RT-PCR assay for simultaneous detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus	29

Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay	29
Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT	26
Evaluation of a surrogate virus neutralization test for high-throughput serosurveillance of SARS-CoV-2	26
SARS-CoV-2 multiplex RT-PCR to detect variants of concern (VOCs) in Malaysia, between January to May 2021	26
Detection of a novel porcine circovirus 4 in Korean pig herds using a loop-mediated isothermal amplification assay	26
Antiviral activity of bioactive phytocompounds against coronavirus: An update	26
Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet	25
Establishment of a one-step reverse transcription recombinase polymerase amplification assay for the detection of potato virus S	24
Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples	24
Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements	23
Comparison of commercial assays and laboratory developed tests for detection of SARS-CoV-2	23
Two novel SARS-CoV-2 surrogate virus neutralization assays are suitable for assessing successful immunization with mRNA-1273	23
A novel single nucleotide polymorphism assay for the detection of N501Y SARS-CoV-2 variants	23
A recombinase polymerase amplification combined with lateral flow dipstick for rapid and specific detection of African swine fever virus	23
Development and validation of a multiplex reverse transcriptase-PCR assay for simultaneous testing of influenza A, influenza B and SARS-CoV-2	22
Comparative analysis of point-of-care, high-throughput and laboratory-developed SARS-CoV-2 nucleic acid amplification tests (NATs)	21
A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit	21
On-field evaluation of a ultra-rapid fluorescence immunoassay as a frontline test for SARS-CoV-2 diagnostic	21
COVID-19: Invasion, pathogenesis and possible cure – A review	20
Screening of PRRSV- and ASFV-encoded proteins involved in the inflammatory response using a porcine iGLuc reporter	20
Next-generation sequencing of human respiratory syncytial virus subgroups A and B genomes	20
Development of a SYBR Green quantitative PCR assay for detection of Lates calcarifer herpesvirus (LCHV) in farmed barramundi	20
Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens	20
Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNA	19
Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction	19
Reflex viral load testing in dried blood spots generated by plasma separation card allows the screening and diagnosis of chronic viral hepatitis	19
Development of immunochromatographic strip assay for rapid detection of novel goose astrovirus	18
Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing	18
Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1	17
Detection of SARS-CoV-2 nucleocapsid antigen from serum can aid in timing of COVID-19 infection	17
Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?	17
Establishment and characterization of a continuous cell line from heart of Nile tilapia Oreochromis niloticus and its susceptibility to tilapia lake virus	17
Simultaneous detection and differentiation of porcine circovirus 3 and 4 using a SYBR Green І-based duplex quantitative PCR assay	17
Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load	17
Exploring alternative swabs for use in SARS-CoV-2 detection from the oropharynx and anterior nares	16
Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation	16
Comparative sensitivity study of primary cells, vero, OA3.Ts and ESH-L cell lines to lumpy skin disease, sheeppox, and goatpox viruses detection and growth	16
Does sampling saliva increase detection of SARS-CoV-2 by RT-PCR? Comparing saliva with oro-nasopharyngeal swabs	16
How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?	16
Analytical performances of five SARS-CoV-2 whole-blood finger-stick IgG-IgM combined antibody rapid tests	15
Environmental and air sampling are efficient methods for the detection and quantification of foot-and-mouth disease virus	15
Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay	15
Optimized protocol for a quantitative SARS-CoV-2 duplex RT-qPCR assay with internal human sample sufficiency control	15
May viral load detected in saliva in the early stages of infection be a prognostic indicator in COVID-19 patients?	15
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex	15
Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1)	15
An optimized and robust SARS-CoV-2 pseudovirus system for viral entry research	14
Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA	14
Performance of the Roche IL-6 chemiluminescent immunoassay in patients with COVID-like respiratory symptoms	14
Development and evaluation of a method for concentration and detection of salmonid alphavirus from seawater	14

Diagnostic techniques for COVID-19: A mini-review	14
Performance evaluation of automated chemiluminescence immunoassay based antigen detection – Moving towards more reliable ways to predict SARS-CoV-2 infection	14
Evaluation of low-cost viral concentration methods in wastewaters: Implications for SARS-CoV-2 pandemic surveillances	14
Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material	13
Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2	13
A new assay for quantitative detection of hepatitis A virus	13
Anti-SARS-CoV-2 IgG antibody response among Indian COVID-19 patients using β-propiolactone-inactivated, whole virus-based indirect ELISA	13
An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing	13
Automated, label-free TCID50 assay to determine the infectious titer of virus-based therapeutics	13
Rapid and sensitive detection of hepatitis B virus by lateral flow recombinase polymerase amplification assay	13
End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19)	13
Clinical usefulness of extraction-free PCR assay to detect SARS-CoV-2	13
Comparative evaluation of six immunoassays for the detection of antibodies against SARS-CoV-2	13
Mouse hepatitis virus: A betacoronavirus model to study the virucidal activity of air disinfection equipment on surface contamination	13
A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator	13
Head-to-head comparison of Enzyme Linked Immunosorbent Assay (ELISA) and Enhanced Chemiluminescence Immunoassay (ECLIA) for the detection of Transfusion Transmitted Disease (TTD) Markers; HIV, HCV and	12
Comparison of the PowerChek SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit and BioFire Respiratory Panel 2.1 for simultaneous detection of SARS-CoV-2, influenza A and B, and respirator	12
Cross comparison of AmpFire HPV genotyping assay and Roche human papillomavirus (HPV) linear array for HPV genotyping of anal swab samples	12
A revisited two-step microtiter plate assay: Optimization of in vitro multiplicity of infection (MOI) for Coliphage and Vibriophage	12
Diagnostic accuracy comparison of three fully automated chemiluminescent immunoassay platforms for the detection of SARS-CoV-2 antibodies	12
SARS-CoV-2 microfluidic antigen point-of-care testing in Emergency Room patients during COVID-19 pandemic	12
Evaluation of spike protein antigens for SARS-CoV-2 serology	12
SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis	11
A review on colorimetric assays for DNA virus detection	11
Optimized formulation buffer preserves adeno-associated virus-9 infectivity after 4 °C storage and freeze/thawing cycling	11
Development of a real-time RT-qPCR assay for the detection of porcine respirovirus 1	11
Carboxamide and N-alkylcarboxamide additives can greatly reduce non specific amplification in Loop-Mediated Isothermal Amplification for Foot-and-Mouth disease Virus (FMDV) using Bst 3.0 polymerase	11
Detection of anti-SFTSV nuclear protein antibody in the acute phase sera of patients using double-antigen ELISA and immunochromatography	11
Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies	11
The evolving direct and indirect platforms for the detection of SARS-CoV-2	11
Multiplexed, microscale, microarray-based serological assay for antibodies against all human-relevant coronaviruses	11
Advanced Lyophilised Loop Mediated Isothermal Amplification (L-LAMP) based point of care technique for the detection of dengue virus	11
SARS-CoV-2 and aerosols—Arguing over the evidence	11
An in-well direct lysis method for rapid detection of SARS-CoV-2 by real time RT-PCR in eSwab specimens	11
Comparison of four PCR and two point of care assays used in the laboratory detection of SARS-CoV-2	11
The Diagnostic Accuracy of Xpert Xpress to SARS-CoV-2: A systematic review	11
A rapid competitive method for bacteriophage genomic DNA extraction	11
Performance of a six-methylation-marker assay on self-collected cervical samples – A feasibility study	11
A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2	11
Quantitative serum HBV markers in predicting phases of natural history of chronic HBV infection	11
Development of a simple genotyping method based on indel mutations to rapidly screen SARS-CoV-2 circulating variants: Delta, Omicron BA.1 and BA.2	11
Application of iron flocculation to concentrate white spot syndrome virus in seawater	11
Development of an indirect ELISA against African swine fever virus using two recombinant antigens, partial p22 and p30	11
Loop-Mediated Isothermal Amplification assays for on-site detection of the main sweetpotato infecting viruses	11
Evaluation of sensitivity and specificity in RNA-Seq-based detection of grapevine viral pathogens	11
A novel real-time PCR to detect Cetacean morbillivirus in Atlantic cetaceans	10
Evaluation of three different concentration and extraction methods for recovery efficiency of human adenovirus and human rotavirus virus A	10
ROTADIAL: The first nanobody-based immunoassay to detect Group A Rotavirus	10
Development and performance characteristics evaluation of a new Bioelectric Recognition Assay (BERA) method for rapid Sars-CoV-2 detection in clinical samples	10
Rapid detection of SARS-CoV-2 variants using allele-specific PCR	10
Evaluation of the analytical performance of six rapid diagnostic tests for the detection of viral hepatitis B and C in Lubumbashi, Democratic Republic of Congo	10
A brief performance evaluation and literature review of Abbott ID Now COVID-19 rapid molecular-based test	10
Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2	10
Proteinase K treatment in absence of RNA isolation classical procedures is a quick and cheaper alternative for SARS-CoV-2 molecular detection	10
Inactivation of SARS-CoV-2 isolates from lineages B.1.1.7 (Alpha), P.1 (Gamma) and B.1.110 by heating and UV irradiation	10
In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR	10
Development of a droplet digital polymerase chain reaction (ddPCR) assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper seeds	10
Assessment of two volumetrically different concentration approaches to improve sensitivities for SARS-CoV-2 detection during wastewater monitoring	10
Development of a duplex real-time reverse transcription-polymerase chain reaction assay for the simultaneous detection of goose astrovirus genotypes 1 and 2	10
A novel method for the detection and diagnosis of virus infections in honey bees	10
Optimization of droplet digital PCR assays for the type-specific detection and quantification of five HPV genotypes, including additional data on viral loads of nine different HPV genotypes in cervica	9
A low-cost simple test for weekly detection of Mycoplasma hyorhinis and arginini contaminations in cell cultures and viral preparations	9
A rapid Focus-Forming Assay for quantification of infectious adenoviral vectors	9
A luciferase immunosorbent assay for quantitative detection of IgG antibodies against SARS-CoV-2 nucleoprotein	9
Rapid and sensitive single-sample viral metagenomics using Nanopore Flongle sequencing	9
Enhanced throughput of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR panel by assay multiplexing and specimen pooling	9
Novel degenerate primer sets for the detection and identification of emaraviruses reveal new chrysanthemum species	9
A mid-throughput HBV replication inhibition assay capable of detecting ribonuclease H inhibitors	9
Comparison of SARS-CoV-2 N gene real-time RT-PCR targets and commercially available mastermixes	9
Evaluation of two RT-PCR techniques for SARS-CoV-2 RNA detection in serum for microbiological diagnosis	9
Diagnosis of Monkeypox infection: Validation of two diagnostic kits for viral detection using RT-PCR	9
A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions	9
Evaluation of virucidal activity of fabrics using feline coronavirus	9
Accessioning and automation compatible anterior nares swab design	9
The theory and practice of the viral dose in neutralization assay: Insights on SARS-CoV-2 “doublethink” effect	9
Rapid detection of porcine deltacoronavirus and porcine epidemic diarrhea virus using the duplex recombinase polymerase amplification method	9
Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics	9
A new single-chain variable fragment (scFv) antibody provides sensitive and specific detection of citrus tristeza virus	9
Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein	9
Validation of the analytical performance of nine commercial RT-qPCR kits for SARS-CoV-2 detection using certified reference material	8

Development of singleplex and multiplex real-time (Taqman®) RT-PCR assays for the detection of viruses associated with fig mosaic disease	8
Development and evaluation of one-step real-time RT-PCR assay for improved detection of foot-and-mouth disease virus serotypes circulating in Egypt	8
A sensitive triple nanoparticle-assisted PCR assay for detection of fowl adenovirus, infectious bursal disease virus and chicken anemia virus	8
Evolving strategy for an evolving virus: Development of real-time PCR assays for detecting all SARS-CoV-2 variants of concern	8
Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA	8
COVID-19 in Hong Kong – Public health, food safety, and animal vectors perspectives	8
Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples	8
Evaluation of the impact of pre-analytical conditions on sample stability for the detection of SARS-CoV-2 RNA	8
Influence of membrane pore-size on the recovery of endogenous viruses from wastewater using an adsorption-extraction method	8
SARS-CoV-2 detection in nasopharyngeal swabs: Performance characteristics of a real-time RT-qPCR and a droplet digital RT-PCR assay based on the exonuclease region (ORF1b, nsp 14)	8
Rapid diagnostic detection of tomato apical stunt viroid based on isothermal reverse transcription-recombinase polymerase amplification	8
Rabies VLPs adjuvanted with saponin-based liposomes induce enhanced immunogenicity mediated by neutralizing antibodies in cattle, dogs and cats	8
Clinical evaluation of the automated Abbott RealTime SARS-CoV-2, Alinity m SARS-CoV-2, and Alinity m Resp-4-Plex assays	8
Analytical and clinical evaluation of four commercial SARS-CoV-2 serological immunoassays in hospitalized patients and ambulatory individuals	8
Detection and quantification of antibody to SARS CoV 2 receptor binding domain provides enhanced sensitivity, specificity and utility	8
Development of a chemiluminescence immunoassay to accurately detect African swine fever virus antibodies in serum	8
Development of diagnostic systems for wide range and highly sensitive detection of two waterborne hepatitis viruses from groundwater using the conventional reverse transcription nested PCR assay	8
Evaluation of extraction and enrichment methods for recovery of respiratory RNA viruses in a metagenomics approach	8
Analysis of external quality assessment samples revealed crucial performance differences between commercial RT-PCR assays for SARS-CoV-2 detection when taking extraction methods and real-time-PCR inst	8
Development of efficient, sensitive, and specific detection method for Encephalomyocarditis virus based on CRISPR/Cas13a	8
The challenge of screening SARS-CoV-2 variants of concern with RT-qPCR: One variant can hide another	8
Reduced amplification efficiency of the RNA-dependent-RNA-polymerase target enables tracking of the Delta SARS-CoV-2 variant using routine diagnostic tests	8
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates	8
Comparison of the AmpFire and MA-6000 polymerase chain reaction platforms for high-risk human papillomavirus testing in cervical precancer screening	7
Magnetic Multiplex Loop Mediated Isothermal Amplification (MM-LAMP) technique for simultaneous detection of dengue and chikungunya virus	7
Performance evaluation of Xpert HBV viral load (VL) assay: Point-of-care molecular test to strengthen and decentralize management of chronic hepatitis B (CHB) infection	7
Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR	7
Evaluation of antigen-based rapid detection test for the diagnosis of SARS CoV-2 in low-income countries	7
MinION nanopore sequencing and assembly of a complete human papillomavirus genome	7
Development of a cellular high-content, immunofluorescent HBV core assay to identify novel capsid assembly modulators that induce the formation of aberrant HBV core structures	7
Comparison of commercial SARS-CoV-2 surrogate neutralization assays with a full virus endpoint dilution neutralization test in two different cohorts	7
Evaluation of the diagnostic accuracy of an affordable rapid diagnostic test for African Swine Fever antigen detection in Lao People’s Democratic Republic	7
Detection of Sri Lankan cassava mosaic virus by loop-mediated isothermal amplification using dried reagents	7
Long amplicon sequencing for improved genetic characterization of African swine fever virus	7
Standardization of a high-performance RT-qPCR for viral load absolute quantification of influenza A	7
Quantitative detection of human adenovirus from river water by monolithic adsorption filtration and quantitative PCR	7
Correlation of dried blood spots and plasma for quantification of Immunoglobulin (IgG) against Receptor binding domain and full length spike protein of SARS-CoV-2	7
Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materials	7
Screening of potential spike glycoprotein / ACE2 dual antagonists against COVID-19 in silico molecular docking	7
A robust, cost-effective and widely applicable whole-genome sequencing protocol for capripoxviruses	7
Development of a multiplex RT-PCR assay for the detection of soybean mosaic virus, bean common mosaic virus and cucumber mosaic virus in field samples of soybean	7
Diminished amplification of SARS-CoV-2 ORF1ab in a commercial dual-target qRT-PCR diagnostic assay	7
Evaluation of extraction and amplification assays for the detection of SARS-CoV-2 at Auckland Hospital laboratory during the COVID-19 outbreak in New Zealand	7