Journal of Virological Methods

Article	Citations
Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit	137
Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay	90
Performance evaluation of the Roche Elecsys Anti-SARS-CoV-2 S immunoassay	78
Comparison of potency assays to assess SARS-CoV-2 neutralizing antibody capacity in COVID-19 convalescent plasma	73
The screening value of RT-LAMP and RT-PCR in the diagnosis of COVID-19: systematic review and meta-analysis	59
Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater	54
Analysis of preferred codon usage in the coronavirus N genes and their implications for genome evolution and vaccine design	53
A combined approach of MALDI-TOF mass spectrometry and multivariate analysis as a potential tool for the detection of SARS-CoV-2 virus in nasopharyngeal swabs	52
SARS-CoV-2: Comparative analysis of different RNA extraction methods	50
Combination of serological total antibody and RT-PCR test for detection of SARS-COV-2 infections	49
The effect of heat-treatment on SARS-CoV-2 viability and detection	47
Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay	47
Genomic and evolutionary comparison between SARS-CoV-2 and other human coronaviruses	46
Evaluation of the QuantiFERON SARS-CoV-2 interferon-ɣ release assay in mRNA-1273 vaccinated health care workers	37
Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2	36
Comparison analysis of different swabs and transport mediums suitable for SARS-CoV-2 testing following shortages	36
Brief validation of the novel GeneXpert Xpress SARS-CoV-2 PCR assay	33
Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2	33
Isothermal recombinase polymerase amplification-lateral flow detection of SARS-CoV-2, the etiological agent of COVID-19	32
Time series analysis and predicting COVID-19 affected patients by ARIMA model using machine learning	32
Reliable detection of SARS-CoV-2 with patient-collected swabs and saline gargles: A three-headed comparison on multiple molecular platforms	31
Sample pooling strategies for SARS-CoV-2 detection	28
Establishment of an SYBR Green-based real-time PCR assay for porcine circovirus type 4 detection	28
Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay	27
Preliminary optimisation of a simplified sample preparation method to permit direct detection of SARS-CoV-2 within saliva samples using reverse-transcription loop-mediated isothermal amplification (RT	26

Development a multiplex RT-PCR assay for simultaneous detection of African swine fever virus, classical swine fever virus and atypical porcine pestivirus	26
Comparison of two commercial surrogate ELISAs to detect a neutralising antibody response to SARS-CoV-2	26
Improving human coronavirus OC43 (HCoV-OC43) research comparability in studies using HCoV-OC43 as a surrogate for SARS-CoV-2	26
Development of a triplex real-time PCR assay for detection and differentiation of gene-deleted and wild-type African swine fever virus	25
Determination of SARS-CoV-2 antibodies with assays from Diasorin, Roche and IDvet	25
SARS-CoV-2 multiplex RT-PCR to detect variants of concern (VOCs) in Malaysia, between January to May 2021	25
Serological and molecular study of Crimean-Congo Hemorrhagic Fever Virus in cattle from selected districts in Uganda	24
Madin-Darby bovine kidney (MDBK) cells are a suitable cell line for the propagation and study of the bovine poxvirus lumpy skin disease virus	23
Detection of a novel porcine circovirus 4 in Korean pig herds using a loop-mediated isothermal amplification assay	23
Comparison of commercial assays and laboratory developed tests for detection of SARS-CoV-2	23
Establishment of a one-step reverse transcription recombinase polymerase amplification assay for the detection of potato virus S	23
A novel single nucleotide polymorphism assay for the detection of N501Y SARS-CoV-2 variants	22
A recombinase polymerase amplification combined with lateral flow dipstick for rapid and specific detection of African swine fever virus	22
Antiviral activity of bioactive phytocompounds against coronavirus: An update	22
Two novel SARS-CoV-2 surrogate virus neutralization assays are suitable for assessing successful immunization with mRNA-1273	22
An Immunoperoxidase Monolayer Assay (IPMA) for the detection of lumpy skin disease antibodies	21
Comparative analysis of point-of-care, high-throughput and laboratory-developed SARS-CoV-2 nucleic acid amplification tests (NATs)	21
A simple method for SARS-CoV-2 detection by rRT-PCR without the use of a commercial RNA extraction kit	20
Evaluation of three extraction-free SARS-CoV-2 RT-PCR assays: A feasible alternative approach with low technical requirements	20
Evaluation of a surrogate virus neutralization test for high-throughput serosurveillance of SARS-CoV-2	20
Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples	20
Development and validation of a multiplex reverse transcriptase-PCR assay for simultaneous testing of influenza A, influenza B and SARS-CoV-2	19
Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNA	19
On-field evaluation of a ultra-rapid fluorescence immunoassay as a frontline test for SARS-CoV-2 diagnostic	19
Comparative performance of CRISPR-Cas12a assays for SARS-CoV-2 detection tested with RNA extracted from clinical specimens	19
Comparison of Illumina MiSeq and the Ion Torrent PGM and S5 platforms for whole-genome sequencing of picornaviruses and caliciviruses	19
Accuracy of a RT-qPCR SARS-CoV-2 detection assay without prior RNA extraction	18
COVID-19: Invasion, pathogenesis and possible cure – A review	18
Rapid and sensitive detection of high pathogenicity Eurasian clade 2.3.4.4b avian influenza viruses in wild birds and poultry	17
Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) assays for the detection of two novel viru	17
Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing	17
Reflex viral load testing in dried blood spots generated by plasma separation card allows the screening and diagnosis of chronic viral hepatitis	17
Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?	16
Development of a recombinase-aided amplification assay for rapid and sensitive detection of porcine circovirus 3	16
Development of a SYBR Green quantitative PCR assay for detection of Lates calcarifer herpesvirus (LCHV) in farmed barramundi	16
A rapid and sensitive lateral flow immunoassay (LFIA) test for the on-site detection of banana bract mosaic virus in banana plants	16
Establishment and characterization of a continuous cell line from heart of Nile tilapia Oreochromis niloticus and its susceptibility to tilapia lake virus	16
Does sampling saliva increase detection of SARS-CoV-2 by RT-PCR? Comparing saliva with oro-nasopharyngeal swabs	16
Digital PCR assay for the effective detection of COVID-19 patients with SARS-CoV-2 low viral load	16
Evaluation of a portable nanopore-based sequencer for detection of viruses in water	15
Development of immunochromatographic strip assay for rapid detection of novel goose astrovirus	15
A quantitative nuclear egress assay to investigate the nucleocytoplasmic capsid release of human cytomegalovirus	15
Exploring alternative swabs for use in SARS-CoV-2 detection from the oropharynx and anterior nares	15
Analytical performances of five SARS-CoV-2 whole-blood finger-stick IgG-IgM combined antibody rapid tests	15
Screening of PRRSV- and ASFV-encoded proteins involved in the inflammatory response using a porcine iGLuc reporter	15
Development of a recombinase-aided amplification assay for detection of orf virus	15
Optimized protocol for a quantitative SARS-CoV-2 duplex RT-qPCR assay with internal human sample sufficiency control	15
Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay	15
Detection of SARS-CoV-2 nucleocapsid antigen from serum can aid in timing of COVID-19 infection	14
Simultaneous detection and differentiation of porcine circovirus 3 and 4 using a SYBR Green І-based duplex quantitative PCR assay	14

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?	14
A fluorescence-based high throughput-screening assay for the SARS-CoV RNA synthesis complex	14
Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA	14
May viral load detected in saliva in the early stages of infection be a prognostic indicator in COVID-19 patients?	13
Anti-SARS-CoV-2 IgG antibody response among Indian COVID-19 patients using β-propiolactone-inactivated, whole virus-based indirect ELISA	13
Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material	13
Performance evaluation of automated chemiluminescence immunoassay based antigen detection – Moving towards more reliable ways to predict SARS-CoV-2 infection	13
Stabilization of a full-length infectious cDNA clone for duck Tembusu virus by insertion of an intron	13
Screening for protective antigens of Cyprinid herpesvirus 2 and construction of DNA vaccines	12
Development and evaluation of a method for concentration and detection of salmonid alphavirus from seawater	12
Next-generation sequencing of human respiratory syncytial virus subgroups A and B genomes	12
Rapid and sensitive detection of hepatitis B virus by lateral flow recombinase polymerase amplification assay	12
Head-to-head comparison of Enzyme Linked Immunosorbent Assay (ELISA) and Enhanced Chemiluminescence Immunoassay (ECLIA) for the detection of Transfusion Transmitted Disease (TTD) Markers; HIV, HCV and	12
A new assay for quantitative detection of hepatitis A virus	12
Diagnostic accuracy comparison of three fully automated chemiluminescent immunoassay platforms for the detection of SARS-CoV-2 antibodies	12
Performance of the Roche IL-6 chemiluminescent immunoassay in patients with COVID-like respiratory symptoms	12
Diagnostic techniques for COVID-19: A mini-review	12
Evaluation of low-cost viral concentration methods in wastewaters: Implications for SARS-CoV-2 pandemic surveillances	12
A simple colorimetric detection of porcine epidemic diarrhea virus by reverse transcription loop-mediated isothermal amplification assay using hydroxynaphthol blue metal indicator	12
Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation	11
Multiplexed, microscale, microarray-based serological assay for antibodies against all human-relevant coronaviruses	11
SARS-CoV-2 and aerosols—Arguing over the evidence	11
Comparison of the PowerChek SARS-CoV-2, Influenza A&B, RSV Multiplex Real-time PCR Kit and BioFire Respiratory Panel 2.1 for simultaneous detection of SARS-CoV-2, influenza A and B, and respirator	11
Environmental and air sampling are efficient methods for the detection and quantification of foot-and-mouth disease virus	11
Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1	11
Advanced Lyophilised Loop Mediated Isothermal Amplification (L-LAMP) based point of care technique for the detection of dengue virus	11
An in-well direct lysis method for rapid detection of SARS-CoV-2 by real time RT-PCR in eSwab specimens	11
An optimized and robust SARS-CoV-2 pseudovirus system for viral entry research	11
Establishment of a real-time Recombinase Polymerase Amplification (RPA) for the detection of decapod iridescent virus 1 (DIV1)	11
SARS-CoV-2 saliva testing is a useful tool for Covid-19 diagnosis	11
Improved detection of dengue and Zika viruses using multiplex RT-qPCR assays	11
Comparative sensitivity study of primary cells, vero, OA3.Ts and ESH-L cell lines to lumpy skin disease, sheeppox, and goatpox viruses detection and growth	11
Bacteriophage amplification – A comparison of selected methods	11
Evaluation of seven commercial African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples	11
An experimental strategy for preparing circular ssDNA virus genomes for next-generation sequencing	10
The Diagnostic Accuracy of Xpert Xpress to SARS-CoV-2: A systematic review	10
The evolving direct and indirect platforms for the detection of SARS-CoV-2	10
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19	10
Porcine epidemic diarrhea virus: Viral RNA detection and quantification using a validated one-step real time RT-PCR	10
Inactivation of foot-and-mouth disease virus A/IRN/8/2015 with commercially available lysis buffers	10
Carboxamide and N-alkylcarboxamide additives can greatly reduce non specific amplification in Loop-Mediated Isothermal Amplification for Foot-and-Mouth disease Virus (FMDV) using Bst 3.0 polymerase	10
A one-step real-time RT-PCR assay for simultaneous typing of SARS-CoV-2 mutations associated with the E484K and N501Y spike protein amino-acid substitutions	10
A novel real-time PCR to detect Cetacean morbillivirus in Atlantic cetaceans	10
A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2	10
SARS-CoV-2 microfluidic antigen point-of-care testing in Emergency Room patients during COVID-19 pandemic	10
End-point RT-PCR: A potential alternative for diagnosing coronavirus disease 2019 (COVID-19)	10
Development and performance characteristics evaluation of a new Bioelectric Recognition Assay (BERA) method for rapid Sars-CoV-2 detection in clinical samples	10
Evaluation of the analytical performance of six rapid diagnostic tests for the detection of viral hepatitis B and C in Lubumbashi, Democratic Republic of Congo	10
In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2 by RT-qPCR	10
Evaluation of a commercial exogenous internal process control for diagnostic RNA virus metagenomics from different animal clinical samples	10
Clinical usefulness of extraction-free PCR assay to detect SARS-CoV-2	10
Development of a droplet digital polymerase chain reaction (ddPCR) assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper seeds	10
Development of a rapid immunochromatographic strip test for the detection of porcine epidemic diarrhea virus specific SIgA in colostrum	10
Rapid differential detection of genotype I and III Japanese encephalitis virus from clinical samples by a novel duplex TaqMan probe-based RT-qPCR assay	10
Comparative evaluation of six immunoassays for the detection of antibodies against SARS-CoV-2	10
Evaluation of the cobas® HCV test for quantifying HCV RNA in dried plasma spots collected using the cobas® Plasma Separation Card	9
A real-time reverse-transcription isothermal recombinase polymerase amplification assay for the rapid detection of genotype III grass carp (Ctenopharyngodon idella) reovirus	9
Evaluation of spike protein antigens for SARS-CoV-2 serology	9
Rapid detection of porcine deltacoronavirus and porcine epidemic diarrhea virus using the duplex recombinase polymerase amplification method	9
Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2	9
Digital PCR method for detection and quantification of specific antimicrobial drug-resistance mutations in human cytomegalovirus	9
Evaluation of virucidal activity of fabrics using feline coronavirus	9
A low-cost simple test for weekly detection of Mycoplasma hyorhinis and arginini contaminations in cell cultures and viral preparations	9
Development of a real-time RT-qPCR assay for the detection of porcine respirovirus 1	9
A rapid Focus-Forming Assay for quantification of infectious adenoviral vectors	9
Inactivation of SARS-CoV-2 isolates from lineages B.1.1.7 (Alpha), P.1 (Gamma) and B.1.110 by heating and UV irradiation	9
Comparison of four PCR and two point of care assays used in the laboratory detection of SARS-CoV-2	9
Diagnostic accuracy of three SARS-CoV2 antibody detection assays, neutralizing effect and longevity of serum antibodies	9
Comparison of three TaqMan real-time reverse transcription-PCR assays in detecting SARS-CoV-2	9
A brief performance evaluation and literature review of Abbott ID Now COVID-19 rapid molecular-based test	9
The theory and practice of the viral dose in neutralization assay: Insights on SARS-CoV-2 “doublethink” effect	9
Comparison of SARS-CoV-2 N gene real-time RT-PCR targets and commercially available mastermixes	9
A multiplex RT-PCR assay for the simultaneous detection of prevalent viruses infecting pepper (Capsicum annuum L.)	9
Proteinase K treatment in absence of RNA isolation classical procedures is a quick and cheaper alternative for SARS-CoV-2 molecular detection	9
Simultaneous detection of classical PRRSV, highly pathogenic PRRSV and NADC30-like PRRSV by TaqMan probe real-time PCR	9
ROTADIAL: The first nanobody-based immunoassay to detect Group A Rotavirus	9
A revisited two-step microtiter plate assay: Optimization of in vitro multiplicity of infection (MOI) for Coliphage and Vibriophage	8
Development of a simple genotyping method based on indel mutations to rapidly screen SARS-CoV-2 circulating variants: Delta, Omicron BA.1 and BA.2	8
BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes	8
Development of a duplex real-time reverse transcription-polymerase chain reaction assay for the simultaneous detection of goose astrovirus genotypes 1 and 2	8

Rabies VLPs adjuvanted with saponin-based liposomes induce enhanced immunogenicity mediated by neutralizing antibodies in cattle, dogs and cats	8
Detection and quantification of antibody to SARS CoV 2 receptor binding domain provides enhanced sensitivity, specificity and utility	8
Enhanced throughput of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR panel by assay multiplexing and specimen pooling	8
A rapid competitive method for bacteriophage genomic DNA extraction	8
Automated, label-free TCID50 assay to determine the infectious titer of virus-based therapeutics	8
Evaluation of three different concentration and extraction methods for recovery efficiency of human adenovirus and human rotavirus virus A	8
Novel degenerate primer sets for the detection and identification of emaraviruses reveal new chrysanthemum species	8
Analysis of external quality assessment samples revealed crucial performance differences between commercial RT-PCR assays for SARS-CoV-2 detection when taking extraction methods and real-time-PCR inst	8
Evolving strategy for an evolving virus: Development of real-time PCR assays for detecting all SARS-CoV-2 variants of concern	8
Analytical and clinical evaluation of four commercial SARS-CoV-2 serological immunoassays in hospitalized patients and ambulatory individuals	8
Reduced amplification efficiency of the RNA-dependent-RNA-polymerase target enables tracking of the Delta SARS-CoV-2 variant using routine diagnostic tests	8
The challenge of screening SARS-CoV-2 variants of concern with RT-qPCR: One variant can hide another	8
Mouse hepatitis virus: A betacoronavirus model to study the virucidal activity of air disinfection equipment on surface contamination	8
Accessioning and automation compatible anterior nares swab design	8
Development of singleplex and multiplex real-time (Taqman®) RT-PCR assays for the detection of viruses associated with fig mosaic disease	8
Evaluation of two RT-PCR techniques for SARS-CoV-2 RNA detection in serum for microbiological diagnosis	8
Production and use of encapsidated RNA mimics as positive control reagents for SARS-CoV-2 RT-qPCR diagnostics	8
Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA	8
Development of an indirect ELISA against African swine fever virus using two recombinant antigens, partial p22 and p30	8
Detection of anti-SFTSV nuclear protein antibody in the acute phase sera of patients using double-antigen ELISA and immunochromatography	8
Loop-Mediated Isothermal Amplification assays for on-site detection of the main sweetpotato infecting viruses	8
Development and evaluation of one-step real-time RT-PCR assay for improved detection of foot-and-mouth disease virus serotypes circulating in Egypt	7
Performance of a six-methylation-marker assay on self-collected cervical samples – A feasibility study	7
Application of iron flocculation to concentrate white spot syndrome virus in seawater	7
Performance evaluation of Xpert HBV viral load (VL) assay: Point-of-care molecular test to strengthen and decentralize management of chronic hepatitis B (CHB) infection	7
Development and application of a combined molecular and tissue culture-based approach to detect latent infectious laryngotracheitis virus (ILTV) in chickens	7
Rapid detection of human respiratory syncytial virus A and B by duplex real-time RT-PCR	7
Optimization of droplet digital PCR assays for the type-specific detection and quantification of five HPV genotypes, including additional data on viral loads of nine different HPV genotypes in cervica	7
Validation of the analytical performance of nine commercial RT-qPCR kits for SARS-CoV-2 detection using certified reference material	7
SARS-CoV-2 detection in nasopharyngeal swabs: Performance characteristics of a real-time RT-qPCR and a droplet digital RT-PCR assay based on the exonuclease region (ORF1b, nsp 14)	7
Rapid detection of SARS-CoV-2 variants using allele-specific PCR	7
Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples	7
A new inactivation method to facilitate cryo-EM of enveloped, RNA viruses requiring high containment: A case study using Venezuelan Equine Encephalitis Virus (VEEV)	7
Rapid diagnostic detection of tomato apical stunt viroid based on isothermal reverse transcription-recombinase polymerase amplification	7
A mid-throughput HBV replication inhibition assay capable of detecting ribonuclease H inhibitors	7
Development of RT-qPCR assays for the detection of three latent viruses of pome	7
Establishment of a porcine parvovirus (PPV) LAMP visual rapid detection method	7
Capillary electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2	7
Development of a multiplex RT-PCR assay for the detection of soybean mosaic virus, bean common mosaic virus and cucumber mosaic virus in field samples of soybean	7