OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Letters is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-02-01 to 2025-02-01.)

Article	Citations
Design of fusion protein for efficient preparation of cyanovirin-n and rapid enrichment of pseudorabies virus	95
Cell density detection based on a microfluidic chip with two electrode pairs	56
Enhancing transcription in Escherichia coli and Pseudomonas putida using bacteriophage lambda anti-terminator protein Q	28
Network pharmacological evaluation of strigolactones efficacy as potential inhibitors against therapeutic targets of hepatocellular carcinoma	25
Recent advances and current challenges in CAR-T cell therapy	24
Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production	23
Economic optimization of expression of soluble human epidermal growth factor in Escherichia coli	23
Low CO2 partial pressure steers CHO cells into a defective metabolic state	22
Investigation of osteogenesis and angiogenesis in perfusion bioreactors using improved multi-layer PCL-nHA-nZnO electrospun scaffolds	22
Oligo replication advantage driven by GC content and Gibbs free energy	22
Generation of stable cell lines using readthrough expression from lentiviral integration	20
A novel spray bioreactor for the proliferation of plant callus; Hyoscyamus niger and Arnebia pulchra	18
Infected insect gut reveals differentially expressed proteins for cellular redox, metal resistance and secretion system in Yersinia enterocolitica-Helicoverpa armigera pathogenic model	18
Novel small multidrug resistance protein Tmt endows the Escherichia coli with triphenylmethane dyes bioremediation capability	17
Synthesis of nano hydroxyapatite from Hypopthalmichthys molitrix (silver carp) bone waste by two different methods: a comparative biophysical and in vitro evaluation on osteoblast MG63 cell lines	17
Directed evolution of cholesterol oxidase with improved thermostability using error-prone PCR	17
The repertoire and levels of secondary metabolites in microbial cocultures depend on the inoculation ratio: a case study involving Aspergillus terreus and Streptomyces rimosus	17
Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae	16
Adenine base-editing-mediated exon skipping induces gene knockout in cultured pig cells	16
Screening and heterologous expression of flavone synthase and flavonol synthase to catalyze hesperetin to diosmetin	16
Differentiation, maturation, and collection of THP-1-derived dendritic cells based on a PEG hydrogel culture platform	15
Facile engineering of aptamer-coupled silk fibroin encapsulated myogenic gold nanocomposites: investigation of antiproliferative activity and apoptosis induction	14
Mutations in the non-structural protein coding region regulate gene expression from replicon RNAs derived from Venezuelan equine encephalitis virus	14
Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus	13
Metabolic engineering of Escherichia coli for the production of (R)-α-lipoic acid	13

A novel recombinant protein vaccine containing the different E7 proteins of the HPV16, 18, 6, 11 E7 linked to the HIV-1 Tat (47–57) improve cytotoxic immune responses	13
Nitrate modulation of Bacillus sp. biofilm components: a proposed model for sustainable bioremediation	12
Long term culturing of CHO cells: phenotypic drift and quality attributes of the expressed monoclonal antibody	12
Three-dimensional cell culture (3DCC) improves secretion of signaling molecules of mesenchymal stem cells (MSCs)	12
Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration	12
Anaerobic biofilm enriched with an ammonia tolerant methanogenic consortium to improve wastewater treatment in the fishing industry	12
CD44 mediates hyaluronan to promote the differentiation of human amniotic mesenchymal stem cells into chondrocytes	11
Role of Hfq in glucose utilization, biofilm formation and quorum sensing system in Bacillus subtilis	11
Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation	11
Multi-pathogen based chimeric vaccine to fight against COVID-19 and concomitant coinfections	11
Taguchi array optimization of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for sensitive and rapid detection of dengue virus serotype 2	11
Simple stain-free screening method for pectinolytic microorganisms under alkalophilic conditions	11
Activation of galactose utilization by the addition of glucose for the fermentation of agar hydrolysate using Lactobacillus brevis ATCC 14869	11
Trichoderma longibrachiatum and thermothelomyces thermophilus co-culture: improvement the saccharification profile of different sugarcane bagasse varieties	10
Recent developments in miRNA based recombinant protein expression in CHO	10
Enhancement of riboflavin production in Bacillus subtilis via in vitro and in vivo metabolic engineering of pentose phosphate pathway	10
Listeria monocytogenes is a solvent tolerant organism secreting a solvent stable lipase: potential biotechnological applications	10
Comparative transcriptome analysis reveals the regulation network for fiber strength in cotton	10
Combination of atmospheric and room temperature plasma (ARTP) mutagenesis, genome shuffling and dimethyl sulfoxide (DMSO) feeding to improve FK506 production in Streptomyces tsukubaensis	10
Three-dimensional tissue fabrication system by co-culture of microalgae and animal cells for production of thicker and healthy cultured food	10
Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett–Burman design combined with response surface methodology	9
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system	9
Development of Schizochytrium sp. strain HS01 with high-DHA and low-saturated fatty acids production by multi-pronged adaptive evolution	9
Mesenchymal stem cell-derived cell-free technologies: a patent landscape	9
Enhancement of cordycepin production from Cordyceps militaris culture by epigenetic modification	8
Recombinant expression, downstream optimization, and therapeutic evaluation of recombinant human interleukin-37 for cancer therapy	8
Production of phenylpyruvic acid by engineered l-amino acid deaminase from Proteus mirabilis	8
Genetic diversity assessment of Palestinian safflower (Carthamus tinctorius L.) utilizing DAMD molecular markers	8
Lentilactobacillus farraginis FSI (3): a whole cell biocatalyst for the synthesis of kojic acid derivative under aquatic condition	8
Role of the mitochondrial citrate-oxoglutarate carrier in lipid accumulation in the oleaginous fungus Mortierella alpina	8
Expression of an immunocomplex consisting of Fc fragment fused with a consensus dengue envelope domain III in Saccharomyces cerevisiae	8
Synthesis of β-ionone from xylose and lignocellulosic hydrolysate in genetically engineered oleaginous yeast Yarrowia lipolytica	8
High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation	7
Efficient strategies to enhance plasmid stability for fermentation of recombinant Escherichia coli harboring tyrosine phenol lyase	7
Construction of a food-grade gene editing system based on CRISPR-Cas9 and its application in Lactococcus lactis NZ9000	7
Uncoupling glucose sensing from GAL metabolism for heterologous lactose fermentation in Saccharomyces cerevisiae	7
Carbohydrate analysis of Mortierella alpina by colorimetry and HPLC–ELSD to reveal accumulation differences of sugar and lipid	7
Isolation of delignifying bacteria and optimization of microbial pretreatment of biomass for bioenergy	7
Functional characterization of a cold related flavanone 3-hydroxylase from Tetrastigma hemsleyanum: an in vitro, in silico and in vivo study	7
A critical appraisal of humanized alternatives to fetal bovine serum for clinical applications of umbilical cord derived mesenchymal stromal cells	7
Comparative assessment of chondral defect repair using human bone marrow- and adipose tissue-derived mesenchymal stem cells, adult and foetal articular cartilage-derived chondrocytes, and chondroproge	7
Facile synthesis of 2-hydroxyacetophenone from racemic styrene oxide catalyzed by engineered enzymes	7
Correction to: The evolution of CRISPR/Cas9 and their cousins: hope or hype?	7
Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors	7
Biosynthetic process and strain improvement approaches for industrial penicillin production	7
Mutational analysis of pig tissue factor pathway inhibitor α to increase anti-coagulation activity in pig-to-human xenotransplantation	7
The potential of extracellular biopolymer production by Mesorhizobium sp. from monosaccharide constituents of lignocellulosic biomass	7
Correction to: Identification of appropriate reference genes for human mesenchymal stem cell analysis by quantitative real-time PCR	7
A strain of Vibrio alginolyticus isolated from Azumapecten farreri and its pathogenic mechanism using CRISPR-Cas9 technology	6
Improved the in-situ remediation effect of benthic microbial electrochemical system by optimizing the anode structure	6

Genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and curing of the endogenous plasmid pBV01	6
Production of ginsenoside compound K by microbial cell factory using synthetic biology-based strategy: a review	6
Heterologous expression, characterization and evolution prediction of a diaphorase from Geobacillus sp. Y4.1MC1	6
Performance of a combined electrotrophic and electrogenic biofilm operated under long-term, continuous cycling	6
Biosynthesis of valerenic acid by engineered Saccharomyces cerevisiae	6
Retraction Note: miR-599 inhibits proliferation and invasion of glioma by targeting periostin	6
Determining the linear correlation between dielectric spectroscopy and viable biomass concentration in filamentous fungal fermentations	6
Improvement of the catalytic performance of glycerol kinase from Bacillus subtilis by chromosomal site-directed mutagenesis	6
An uncharacterized protein from the metagenome with no obvious homology to known lipases shows excellent alkaline lipase properties and potential applications in the detergent industry	6
Biosensors for the detection of organophosphate exposure by a new diethyl thiophosphate-specific aptamer	6
Mycelial culture extracts of selected wood-decay mushrooms as a source of skin-protecting factors	6
Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?	6
Genetically engineered E. coli invade epithelial cells and transfer their genetic cargo into the cells: an approach to a gene delivery system	5
RNA-binding protein Hfq plays a vital role in cellulose decomposition throughout affecting cellulase gene expression	5
Biotransformation of C20- and C22-polyunsaturated fatty acids to 11S- and 13S-hydroxy fatty acids by Escherichia coli expressing 11S-lipoxygenase from Enhygromyxa salina	5
Enhanced enzyme thermostability of a family I.3 lipase LipSR1 by T118A mutation at the calcium-binding site	5
Construction of a mutant Bacillus subtilis strain for high purity poly-γ-glutamic acid production	5
Nitrile hydratase as a promising biocatalyst: recent advances and future prospects	5
Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation	5
Optimization of ultrasound-assisted production of ergosterol from Penicillium brevicompactum by Taguchi statistical method	5
Effects of different growth patterns of Tamarix chinensis on saline-alkali soil: implications for coastal restoration and management	5
Improved preculture management for Cupriavidus necator cultivations	5
Biofilm characterization in removal of total chemical oxygen demand and nitrate from wastewater using draft tube spouted bed reactor	5
A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora	5
Enhanced whole-cell biotransformation of 3-chloropropiophenone into 1-phenyl-1-propanone by hydrogel entrapped Chlorella emersonii (211.8b)	5
Optimizing microbial electrolysis cell performance: strategies to mitigate electron mediator degradation on membranes	5
Enhanced hypoxanthine utilization for cAMP salvage synthesis efficiently by Arthrobacter sp. CCTCC 2013431 via xanthine oxidase inhibition	5
Lactobacillus spp. isolated from prebiotic-derived raw goat milk: probiotic characteristics, cholesterol assimilation and folate production	5
Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus	5
Simple chitin-based cell culture platform for production of biopharmaceuticals	5
Treatments for the amelioration of persistent factors in complex anal fistula	5
Overexpression of a pearl millet WRKY transcription factor gene, PgWRKY74, in Arabidopsis retards shoot growth under dehydration and salinity-stressed conditions	5
Strategy to reduce acetic acid in sugarcane bagasse hemicellulose hydrolysate concomitantly with xylitol production by the promising yeast Cyberlindnera xylosilytica in a bioreactor	5
Squalene hopene cyclases and oxido squalene cyclases: potential targets for regulating cyclisation reactions	5
Preparation of white rot fungal inoculum and its application to bioremediation of chlorimuron-ethyl-contaminated soil	5
Application of exogenous melatonin in vitro and in planta: a review of its effects and mechanisms of action	5
Enhanced β-carotene production by overexpressing the DID2 gene, a subunit of ESCRT complex, in engineered Yarrowia lipolytica	5
Effect of magnesium oxide nanoparticles and LED irradiation on the viability and differentiation of human stem cells of the apical papilla	5
Matrine-producing endophytic fungus Galactomyces candidum TRP-7: screening, identification, and fermentation conditions optimization for Matrine production	5
Diffusion-driven fed-batch fermentation in perforated ring flasks	4
MiR-103/miR-107 inhibits enterovirus 71 replication and facilitates type I interferon response by regulating SOCS3/STAT3 pathway	4
Biological synthesis of nicotinamide mononucleotide	4
Neutralizing anti-diphtheria toxin scFv produced by phage display	4
AmyJ33, a truncated amylase with improved catalytic properties	4
Paddy straw saccharification using immobilized laccase on magnetized multiwall carbon nanotubes	4
Foamed glass ceramics—an upcycled scaffold for microbial biofilm development	4
Straw lignin degradation by lignin peroxidase from Irpex lacteus cooperated with enzymes and small molecules	4
Larvicidal activity against Aedes aegypti and molecular docking studies of compounds extracted from the endophytic fungus Aspergillus sp. isolated from Bertholletia excelsa Humn. & Bonpl	4
Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae	4
Efficient production of d-glucosamine by diacetylchitobiose deacetylase catalyzed deacetylation of N-acetyl-d-glucosamine	4
Autophagy induced by Schwann cell-derived exosomes promotes recovery after spinal cord injury in rats	4
Construction of a rAAV-SaCas9 system expressing eGFP and its application to improve muscle mass	4
Potential of Mortierellaceae for polyunsaturated fatty acids production: mini review	4
Engineering synthetic microbial consortium for cadaverine biosynthesis from glycerol	4
A novel method for the in vitro assembly of virus-like particles and multimeric proteins	4
Organic materials with high C/N ratio: more beneficial to soil improvement and soil health	4
Proteome profiling, biochemical and histological analysis of diclofenac-induced liver toxicity in Yersinia enterocolitica and Lactobacillus fermentum fed rat model: a comparative analysis	4
Effects of the presence of phosphate buffer solution on removal efficiency of Pb and Zn in soil by solid phase microbial fuel cells	4
Cloning and expression of recombinant human superoxide dismutase 1 (hSOD1) in Bacillus subtilis 1012	4
Surfactants: physicochemical interactions with biological macromolecules	4
Superoxide dismutase as multipotent therapeutic antioxidant enzyme: Role in human diseases	4
Synthesis of plant-based, self-adjuvanted, dual antigen specific to Mycobacterium tuberculosis as a novel tuberculosis subunit vaccine that elicits immunogenicity in rabbit	4
Transesterification with CE15 glucuronoyl esterase from Cerrena unicolor reveals substrate preferences	4
Silver nanoparticles induced with aqueous black carpenter ant extract selectively inhibit the growth of Pseudomonas aeruginosa	4
Highly efficient biosynthesis of salidroside by a UDP-glucosyltransferase-catalyzed cascade reaction	4
Cellulose-fueled microbial fuel cells equipped with a bipolar membrane using hydrogen phosphate as the final electron acceptor	4
A temperature-induced chitosanase bacterial cell-surface display system for the efficient production of chitooligosaccharides	4