OOIR: Observatory of International Research

Papers

(The TQCC of Biotechnology Letters is 6. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-08-01 to 2026-08-01.)

Article	Citations
Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors	60
Listeria monocytogenes is a solvent tolerant organism secreting a solvent stable lipase: potential biotechnological applications	41
Recombinant expression, downstream optimization, and therapeutic evaluation of recombinant human interleukin-37 for cancer therapy	33
Cell density detection based on a microfluidic chip with two electrode pairs	32
Affinity chromatography studies for Mespilus germanica L. polyphenol oxidase	25
Silica nanoparticles enhance interfacial self-adherence of a multi-layered extracellular matrix scaffold for vascular tissue regeneration	22
Directed evolution of cholesterol oxidase with improved thermostability using error-prone PCR	21
Effects of three light–dark cycles on circadian rhythm-related genes and photosynthetic parameters in carrot	20
Synthesis of β-ionone from xylose and lignocellulosic hydrolysate in genetically engineered oleaginous yeast Yarrowia lipolytica	20
Design and application of expression constructs for FMDV serotype O structural proteins	19
The pedal-like loop of (R)-selective transaminases plays a critical role to the functionality of the enzyme	19
Antisense oligonucleotides: targeting oncogenic long non-coding RNAs for cancer therapy	19
Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation	19
High throughput screening and metabolic engineering of Saccharopolyspora spinosa for spinosad production	18
Dual functions of nonionic surfactant Tween-20 in enhancing productivity and elution efficiency of alkylresorcinols from Azotobacter vinelandii	17
Screening of a xylanase-producing Trichoderma strain and optimization of its enzyme production conditions	16
Squalene hopene cyclases and oxido squalene cyclases: potential targets for regulating cyclisation reactions	15
Application of lactose based autoinduction for heterologous production of an active [NiFe] hydrogenase in E. coli	15
Paenibacillus barengoltzii: isolation, growth, and characterization of a high dextrinizer α-amylase	15
Enhancing leucine aminopeptidase activity in Aspergillus oryzae LL1 through multi-round UV irradiation mutagenesis with potential application in debittering	15
Recombinant erythropoietin expression elevates by ubiquitous chromatin opening element (UCOE) in CHO DG44 cells	15
Synergistic anticancer effects of interleukin-21 combined with therapeutic peptides in multiple cancer cells	15
Characterization and substrate specificity study of the novel (R)-amine transaminase MagAT	14
Biocontrol of strawberry Botrytis gray mold and prolong the fruit shelf-life by fumigant Trichoderma spp.	14
Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells	14

Sustainable biodegradation of phenol using peroxidases extracted from local agricultural wastes	14
Antioxidant potential and metabolic profiling of Viola odorata and the endophytic Rhizobium sp. BR7	14
Molecular cloning and modelling of a thermostable α-amylase from a thermophilic Geobacillus sp. DS3	13
A rapid analytical method using loop-mediated isothermal amplification (LAMP): visual detection of cry1Ab gene in genetically modified maize seeds and food products	13
Retraction Note: Autophagy regulated by lncRNA HOTAIR contributes to the cisplatin-induced resistance in endometrial cancer cells	13
Enhanced gibberellic acid production in Fusarium fujikuroi through ARTP-EMS mutagenesis and fermentation optimization	12
Clinical and mechanistic insights into biomedical application of Se-enriched probiotics and biogenic selenium nanoparticles	12
Metabolic response of Bacillus spp. to heavy metal stress: pathway alterations and metabolite profiles	12
Optimizing hyaluronic acid production by Streptococcus zooepidemicus using taguchi method: effects of temperature and pH	11
Evaluation of cultivation performance of S. cerevisiae strains expressing GLP-1-GIP peptide precursors	11
Gene expression profiling to elucidate the promotive effects of the volatile organic compound 3-octanone on the mycelial growth of Ganoderma lucidum	11
Identification of laccase genes in Trichoderma asperellum Ts93	11
Enhancing lipase activity in Aspergillus niger through CRISPR/Cas9-mediated protease gene knockout and fermentation optimization	11
BMSCs improve TNBS-induced colitis in rats by inducing Treg differentiation by expressing PD-L1	11
Enhancement hispolon production from Phellinus linteus via epigenetic-modified culture to inhibit human breast cancer cells	11
Isolation, microscopic and magnetotactic characterization of Magnetospirillum moscoviense MS-24 from Banjosa Lake, Pakistan	11
Retraction Note: TP53INP1 3′-UTR functions as a ceRNA in repressing the metastasis of glioma cells by regulating miRNA activity	11
Succession from acetoclastic to hydrogenotrophic microbial community during sewage sludge anaerobic digestion for bioenergy production	10
Gallic acid as biofilm inhibitor can improve transformation efficiency of Ruminiclostridium papyrosolvens	10
Production of mannosylated hrsACE2 by baculovirus expression system; the way to remove and redirect SARS-CoV-2 viral particles to macrophages	10
Efficient production of L-asparaginase using recombinant Pichia pastoris and strategy to eliminate glutaminase activity	10
Research on the application of heterotrophic nitrification-aerobic denitrification bacteria in membrane bioreactor (MBR)	10
Therapeutic potential of a novel IP-10-(anti-HER2 scFv) fusion protein for the treatment of HER2-positive breast cancer	10
Substrate type and CO2 addition significantly influence succinic acid production of Basfia succiniciproducens	10
Combining biotechnology with sustainability: feasibility of bioenergy sorghum in generating high-value bioproducts	10
Antagonistic potential of medicinal mushrooms against pathogenic Ganoderma orbiforme in oil palm using a modified air-lift bioreactor (MALB)	10
Ultrasonic high efficiency preparation of arctigenin from the fruits of Arctium lappa L using Aspergillus niger with deep eutectic solvent assisted	10
Correction: Biodegradation of ketoprofen using a microalgal–bacterial consortium	10
Eco-friendly azo dye removal via a robust laccase from Acinetobacter baumannii KMSP005: a thermo-alkaliphilic biocatalytic approach	10
Magnetic particle-based chemiluminescence immunoassay for serum human heart-type fatty acid binding protein measurement	10
Role of microbial pesticides in pest control for lepidopteran: research status and prospect	10
Measurement of HbA1c with a new phase function using RBC phase image from a low-cost digital holographic microscopy	9
Advancing the frontier of plant-based therapeutics: critical innovations in molecular farming and bioprocess Integration	9
Enhancing production of nicotinamide mononucleotide by metabolic engineering and flow cytometry screening of Escherichia coli	9
The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli	9
Micro-electrochemical DO sensor with ultra-micropore matrix fabricated with femtosecond laser processing successfully applied in on-line DO monitoring for yeast culture	9
Characterization of a novel Escherichia coli recombineering selection/counterselection cassette	9
Droplet based microfluidic and miniaturization at high-throughput level to screening heterologous secondary metabolites in microbes: A critical review	9
Novel TdsD nitroreductase: characterization of kinetics and substrate specificity	9
Preparation, characterization, and enzymatic properties of laccase immobilized on MnO2 nanoparticles via adsorption method	9
An artificial multienzyme cascade for the whole-cell synthesis of rare ketoses from glycerol	9
Toxicants improve glycerol production in the fermentation of undetoxified hydrolysate by Candida glycerinogenes	9
Bone marrow mesenchymal stem cell-derived exosomes loaded with miR-26a through the novel immunomodulatory peptide DP7-C can promote osteogenesis	9
Optimization of astaxanthin production by Rhodotorula toruloides CB6-10/1 using response surface methodology and its genome analysis	8
Construction of a bacteriophage-derived vector with potential applications in targeted drug delivery and cell imaging	8
Development of a synthetic transcription factor-based S-adenosylmethionine biosensor in Saccharomyces cerevisiae	8
The variation of microorganisms and organics during methane production from lignite under an electric field	8
Improving expression of Photobacterium sp. JT-ISH-224 α2,6-sialyltransferase using ProteinMPNN and machine learning guided combinatory mutagenesis	8
Aptamer-functionalized MOFs and AI-driven strategies for early cancer diagnosis and therapeutics	8
Improving the specific activity and stability of alkaline pectinase PEL3 through SpyTag/SpyCatcher cyclization	8

Investigation of osteogenesis and angiogenesis in perfusion bioreactors using improved multi-layer PCL-nHA-nZnO electrospun scaffolds	7
Optimization of ultrasound-assisted production of ergosterol from Penicillium brevicompactum by Taguchi statistical method	7
Differentiation, maturation, and collection of THP-1-derived dendritic cells based on a PEG hydrogel culture platform	7
Optimizing microbial electrolysis cell performance: strategies to mitigate electron mediator degradation on membranes	7
Molecular cloning and characterization of an alpha-amylase inhibitor (TkAAI) gene from Trichosanthes kirilowii Maxim.	7
Trichoderma longibrachiatum and thermothelomyces thermophilus co-culture: improvement the saccharification profile of different sugarcane bagasse varieties	7
Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus	7
Dynamic cultivation of human mesenchymal stem/stromal cells for the production of extracellular vesicles in a 3D bioreactor system	7
Economic optimization of expression of soluble human epidermal growth factor in Escherichia coli	7
A flexible, cost- and time-saving method for in-house laboratory production of DNA markers	7
Genome-scale metabolic model-based engineering of Escherichia coli enhances recombinant single-chain antibody fragment production	7
Enhanced biodiesel production from Fusarium oxysporum via mutagenesis and process optimization using bagasse hydrolysate as a sustainable carbon source	7
Enhancing HAP3 mediated xylose metabolism and acetic acid metabolism promotes lipid production from lignocellulosic hydrolysate fermented by Candida glycerinogenes	7
A novel P450 enzyme assay utilizing an NADP+-based biosensor	7
Mutational analysis of pig tissue factor pathway inhibitor α to increase anti-coagulation activity in pig-to-human xenotransplantation	7
Improved thermostability of Thermobifida fusca mannanase obtained by error-prone PCR mutagenesis	7
Foliar and root absorption of LDH nanosheets loaded with dsRNAs efficiently silenced the expression of whitefly Bemisia tabaci Laccase 1 and Vitellogenin Receptor genes for their effective control	7
Preparation of white rot fungal inoculum and its application to bioremediation of chlorimuron-ethyl-contaminated soil	7
Improving the hydrophilic microenvironment surrounding the catalytic site of fructosyltransferase enhances its catalytic ability	7
Competitive inhibition of a non-natural cofactor dependent formaldehyde dehydrogenase by imidazole	7
Enhancing camptothecin biosynthesis in Camptotheca acuminata through CrLAMT transfer from Catharanthus roseus	7
Establishment of a visual gene knockout system based on CRISPR/Cas9 for the rare actinomycete Nonomuraea gerenzanensis	7
Cellulase and chitinase activities and antagonism against Fusarium oxysporum f.sp. cubense race 1 of six Trichoderma strains isolated from Mexican maize cropping	7
Application of CRISPR-Cas9 in microbial cell factories	7
A single-vector CRISPR/Cas9 system for genome editing and heterologous enzyme secretion in Saccharomyces cerevisiae: a case study on pectate lyase for coffee mucilage removal	6
CRISPR/Cas9-assisted ssDNA recombineering for site-directed mutagenesis and saturation mutagenesis of plasmid-encoded genes	6
Genetic engineering of Beauveria bassiana for enhancing siderophore production and its virulence against the insect hosts	6
Correction: Polyhydroxyalkanoate production by Paramecium caudatum isolated from industrial wastewater: a micro eukaryotic host for bioplastic accumulation	6
Cost-effective production of squalene using Yarrowia lipolytica via metabolic engineering and fermentation engineering	6
Biogenesis and characterization of proficient silver nanoparticles employing marine procured fungi Hamigera pallida and assessment of their antioxidative, antimicrobial and anticancer potency	6
swnk plays an important role in the biosynthesis of swainsonine in Metarhizium anisopliae	6
Recombinant protein CP01850 adjuvanted with Iridea cordata or Sacorpletis skottibergii lipid extracts protected mice against infection by Corynebacterium pseudotuberculosis	6
ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization	6
Antimicrobial activity of thermophilin 110 against the opportunistic pathogen Cutibacterium acnes	6
Pilot-scale production of Bacillus subtilis MSCL 897 spore biomass and antifungal secondary metabolites in a low-cost medium	6
Carrier immobilization and auto-exposition favoring reuse of silyletherase SilE-R from Brassica sp. with high activity and enantiospecificity	6
A gold nanoparticles-based lateral flow assay utilizing baculovirus expressed recombinant nucleocapsid and receptor binding domain proteins for serodetection of IgG and IgM against SARS-CoV-2	6
Homo- and heterofermentative lactobacilli are distinctly affected by furanic compounds	6
Enhanced soluble expression of active recombinant human interleukin-29 using champion pET SUMO system	6
Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production	6
Isolation and characterisation of exosomes from Chinese hamster ovary (CHO) cells	6
A cell-free fluorescence biosensor based on allosteric transcription factor NalC for detection of pentachlorophenol	6
Effects of the deletion and substitution of thioesterase on bacillomycin D synthesis	6
Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology	6
Welan gum promotes camptothecin production in Camptotheca acuminata by activating multiple signaling pathways	6
Quantitative analysis of genome truncation patterns in oversized adeno-associated virus vectors	6