OOIR: Observatory of International Research

Papers

(The TQCC of Enzyme and Microbial Technology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-11-01 to 2024-11-01.)

Article	Citations
A review on preparation and chemical analysis of postbiotics from lactic acid bacteria	97
Plant chitinases and their role in plant defense: A comprehensive review	91
Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis	84
Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29	83
Enzyme immobilized nanomaterials as electrochemical biosensors for detection of biomolecules	70
Decolourization and biodegradation of methylene blue dye by a ligninolytic enzyme-producing Bacillus thuringiensis: Degradation products and pathway	60
Microbial lignin peroxidases: Applications, production challenges and future perspectives	59
Recent advances in the discovery, characterization, and engineering of poly(ethylene terephthalate) (PET) hydrolases	48
Microbial demethylation of lignin: Evidence of enzymes participating in the removal of methyl/methoxyl groups	38
Production and bioprocess optimization of antitumor Epothilone B analogue from Aspergillus fumigatus, endophyte of Catharanthus roseus, with response surface methodology	37
Modified silicates and carbon nanotubes for immobilization of lipase from Rhizomucor miehei: Effect of support and immobilization technique on the catalytic performance of the immobilized biocatalysts	32
Improving the thermostability of a GH11 xylanase by directed evolution and rational design guided by B-factor analysis	31
A review on enzyme-producing lactobacilli associated with the human digestive process: From metabolism to application	31
Decyl esters production from soybean-based oils catalyzed by lipase immobilized on differently functionalized rice husk silica and their characterization as potential biolubricants	30
Bioinspired green synthesis of silver nanoparticles by using a native Bacillus sp. strain AW1-2: Characterization and antifungal activity against Colletotrichum falcatum Went	30
Adsorption of enzymes with hydrolytic activity on polyethylene terephthalate	28
In situ H2O2 generation methods in the context of enzyme biocatalysis	28
Use of biocompatible redox-active polymers based on carbon nanotubes and modified organic matrices for development of a highly sensitive BOD biosensor	27
Biomimetic and biopolymer-based enzyme encapsulation	27
Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of lysine decarboxylase in recombinant Escherichia coli W3110	26
Determination of phenol biodegradation pathways in three psychrotolerant yeasts, Candida subhashii A011, Candida oregonensis B021 and Schizoblastosporion starkeyi-henricii L012, isolated from Rucianka	26
Improving the enzyme property of D-allulose 3-epimerase from a thermophilic organism of Halanaerobium congolense through rational design	25
A novel β-glucosidase from a hot-spring metagenome shows elevated thermal stability and tolerance to glucose and ethanol	25
Role of the lactobacilli in food bio-decontamination: Friends with benefits	25
An enzyme-mediated controlled release system for curcumin based on cyclodextrin/cyclodextrin degrading enzyme	24

Production of indigo through the use of a dual-function substrate and a bifunctional fusion enzyme	24
Enhancement of PET biodegradation by anchor peptide-cutinase fusion protein	23
Highly sensitive detection of glucose via glucose oxidase immobilization onto conducting polymer-coated composite polyacrylonitrile nanofibers	22
Gamma aminobutyric acid (GABA) production in Escherichia coli with pyridoxal kinase (pdxY) based regeneration system	22
Cloning, expression and biochemical characterization of lignin-degrading DyP-type peroxidase from Bacillus sp. Strain BL5	22
Properties of d-allulose 3-epimerase mined from Novibacillus thermophilus and its application to synthesis of d-allulose	21
QQ-PAC core-shell structured quorum quenching beads for potential membrane antifouling properties	21
Enhanced biotransformation of the minor ginsenosides in red ginseng extract by Penicillium decumbens β-glucosidase	21
Enzymatic biosensor based on dendritic gold nanostructure and enzyme precipitation coating for glucose sensing and detection	20
Combinatorial engineering for efficient production of protein-glutaminase in Bacillus subtilis	20
Enzymes in nearly anhydrous deep eutectic solvents: Insight into the biocompatibility and thermal stability	20
Immobilization on magnetic PVA/SA@Fe3O4 hydrogel beads enhances the activity and stability of neutral protease	19
Structural studies of Myceliophthora Thermophila Laccase in the presence of deep eutectic solvents	19
Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation	19
A novel application of simple submersible yeast-based microbial fuel cells as dissolved oxygen sensors in environmental waters	18
Creation of a robust and R-selective ω-amine transaminase for the asymmetric synthesis of sitagliptin intermediate on a kilogram scale	18
Efficient biosynthesis of lacto-N-neotetraose by a novel β-1,4-galactosyltransferase from Aggregatibacter actinomycetemcomitans NUM4039	18
Hyaluronic acid-coated chitosan nanoparticles as carrier for the enzyme/prodrug complex based on horseradish peroxidase/indole-3-acetic acid: Characterization and potential therapeutic for bladder can	18
Cloning and expression analysis of fused holin-endolysin from RL bacteriophage; Exhibits broad activity against multi drug resistant pathogens	17
Thermoplastic starch (TPS) bioplastic, the green solution for single-use petroleum plastic food packaging – A review	17
Preparation of efficient, stable, and reusable copper-phosphotriesterase hybrid nanoflowers for biodegradation of organophosphorus pesticides	17
Characterization of a biosurfactant producing electroactive Bacillus sp. for enhanced Microbial Fuel Cell dye decolourisation	17
Non-covalent and covalent immobilization of papain onto Ti3C2 MXene nanosheets	17
Production and in vitro evaluation of prebiotic manno-oligosaccharides prepared with a recombinant Aspergillus niger endo-mannanase, Man26A	17
Lactobacillus amylovorus derived lipase-mediated silver derivatization over poly(ε-caprolactone) towards antimicrobial coatings	17
Expression and purification of asprosin in Pichia pastoris and investigation of its increase glucose uptake activity in skeletal muscle through activation of AMPK	16
Symbiotic chitin degradation by a novel anaerobic thermophilic bacterium Hydrogenispora sp. UUS1-1 and the bacterium Tepidanaerobacter sp. GT38	16
Engineering the probiotic bacterium Escherichia coli Nissle 1917 as an efficient cell factory for heparosan biosynthesis	16
Characterization of a thermostable phytase from Bacillus licheniformis WHU and further stabilization of the enzyme through disulfide bond engineering	16
Screening and characterization of a GH78 α-l-rhamnosidase from Aspergillus terreus and its application in the bioconversion of icariin to icaritin with recombinant β-glucosidase	15
Preparation of phytic acid modified α-Glucosidase/Cu3(PO4)2·3H2O hybrid nanoflower and its application	15
Electrochemical detection of gram-negative bacteria through mastoparan-capped magnetic nanoparticle	15
Production and characterisation of a novel actinobacterial DyP-type peroxidase and its application in coupling of phenolic monomers	15
Efficient production of lactobionic acid using genetically engineered Pseudomonas taetrolens as a whole-cell biocatalyst	15
Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification	14
Ternary biogenic silica/magnetite/graphene oxide composite for the hyperactivation of Candida rugosa lipase in the esterification production of ethyl valerate	14
Enhancement of carotenoid production and its regulation in edible mushroom Cordyceps militaris by abiotic stresses	14
Biochemical and structural characterization of a novel thermophilic and acidophilic β-mannanase from Aspergillus calidoustus	14
Carbohydrate-binding modules of ChiB and ChiC promote the chitinolytic system of Serratia marcescens BWL1001	14
Use of green light to improve the production of lignocellulose-decay enzymes by Pleurotus spp. in liquid cultivation	14
Improved thermostability, acid tolerance as well as catalytic efficiency of Streptomyces rameus L2001 GH11 xylanase by N-terminal replacement	14
Rational design of Shewanella sp. l-arabinose isomerase for d-galactose isomerase activity under mesophilic conditions	13
Rational engineering of cofactor specificity of glutamate dehydrogenase for poly-γ-glutamic acid synthesis in Bacillus licheniformis	13
Equipment-free quantitative determination of urea based on paper-based sensor via urease-mediated chitosan viscosity change	13
Combinatorial engineering for improved menaquinone-4 biosynthesis in Bacillus subtilis	13
cAMP-PKA and HOG1 signaling pathways regulate liamocin production by different ways via the transcriptional activator Msn2 in Aureobasidium melanogenum	13
A fast and easy strategy for lytic polysaccharide monooxygenase-cleavable His6-Tag cloning, expression, and purification	13
Comparative characterization of glyoxal oxidase from Phanerochaete chrysosporium expressed at high levels in Pichia pastoris and Trichoderma reesei	13
Lipases of Endophytic Fungi Stemphylium lycopersici and Sordaria sp.: Application in the synthesis of solketal derived Monoacylglycerols	13
Nanotechnology based technological development in biofuel production: Current status and future prospects	13

Microbial biosensor for Salmonella using anti-bacterial antibodies isolated from human serum	13
A facile and one-pot aqueous phase transfer of oleylamine capped Au NP with aminophenylboronic acid used as transfer and targeting ligand	13
Immobilization of Thermomyces lanuginosus lipase on a new hydrophobic support (Streamline phenyl™): Strategies to improve stability and reusability	13
Application of magnetic immobilized enzyme of nano dialdehyde starch in deacidification of rice bran oil	13
Methods of detection of β-galactosidase enzyme in living cells	13
An artificial multi-enzyme cascade biocatalysis for biomanufacturing of nicotinamide mononucleotide from starch and nicotinamide in one-pot	13
A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET)	13
Amylosucrase from Deinococcus geothermalis can be modulated under different reaction conditions to produce novel quercetin 4′-O-α-d-isomaltoside	12
The polyhydroxyalkanoate (PHA) synthase 1 of Pseudomonas sp. H9 synthesized a 3-hydroxybutyrate-dominant hybrid of short- and medium-chain-length PHA	12
Design of a PL18 alginate lyase with flexible loops and broader entrance to enhance the activity and thermostability	12
Synthesis of improved long-chain isomaltooligosaccharide, using a novel glucosyltransferase derived from Thermoanaerobacter thermocopriae, with maltodextrin	12
Periplasmic expression of Pseudomonas fluorescens peroxidase Dyp1B and site-directed mutant Dyp1B enzymes enhances polymeric lignin degradation activity in Pseudomonas putida KT2440	12
The effects on antioxidant enzymes of PMMA/hydroxyapatite nanocomposites/composites	12
High D-arabitol production with osmotic pressure control fed-batch fermentation by Yarrowia lipolytica and proteomic analysis under nitrogen source perturbation	12
Cytochrome P450 5208A3 is a promiscuous xenobiotic biotransforming enzyme in Cunninghamella elegans	12
Kinetic and thermodynamic study of laccase cross-linked onto glyoxyl Immobead 150P carrier: Characterization and application for beechwood biografting	12
Production and characterization of novel thermostable CotA-laccase from Bacillus altitudinis SL7 and its application for lignin degradation	12
One-pot production of maltoheptaose (DP7) from starch by sequential addition of cyclodextrin glucotransferase and cyclomaltodextrinase	11
Peptide ligases: A Novel and potential enzyme toolbox for catalytic cross-linking of protein/peptide-based biomaterial scaffolds for tissue engineering	11
Improving (2S)-naringenin production by exploring native precursor pathways and screening higher-active chalcone synthases from plants rich in flavonoids	11
Development of a novel expression platform for heterologous protein production via deleting the p53-like regulator Vib1 in Trichoderma reesei	11
A novel chondroitin AC lyase from Pedobacter xixiisoli: Cloning, expression, characterization and the application in the preparation of oligosaccharides	11
Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola	11
Directed evolution of the transcription factor Gal4 for development of an improved transcriptional regulation system in Saccharomyces cerevisiae	11
Characterizations of the endolysin Lys84 and its domains from phage qdsa002 with high activities against Staphylococcus aureus and its biofilms	11
A wheat bran inducible expression system for the efficient production of α-L-arabinofuranosidase in Bacillus subtilis	11
Metataxonomic characterization of an autochthonous and allochthonous microbial consortium involved in a two-stage anaerobic batch reactor applied to hydrogen and methane production from sugarcane baga	11
Glucose oxidase-mediated sodium alginate gelation: Equipment-Free detection of glucose in fruit samples	11
A novel growth and isolation medium for exoelectrogenic bacteria	11
Cyanide-free synthesis of aromatic nitriles from aldoximes: Discovery and application of a novel heme-containing aldoxime dehydratase	11
Ancestral sequence reconstruction and spatial structure analysis guided alteration of longer-chain substrate catalysis for Thermomicrobium roseum lipase	11
A homodimeric bacterial exo-β-1,3-glucanase derived from moose rumen microbiome shows a structural framework similar to yeast exo-β-1,3-glucanases	11
Construction of lactic acid overproducing Clostridium thermocellum through enhancement of lactate dehydrogenase expression	11
Whole-cell biosynthesis of d-tagatose from maltodextrin by engineered Escherichia coli with multi-enzyme co-expression system	11
Covalent binding of glutathione on magnetic nanoparticles: Application for immobilizing small fragment ubiquitin-like-specific protease 1	10
Rational engineering of a metalloprotease to enhance thermostability and activity	10
Production of prebiotic xylooligosaccharides from arabino- and glucuronoxylan using a two-domain Jonesia denitrificans xylanase from GH10 family	10
Geotrichum candidum aldehyde dehydrogenase-inorganic nanocrystal with enhanced activity	10
Expression of a novel α-glucosidase from Aspergillus neoniger in Pichia pastoris and its efficient recovery for synthesis of isomaltooligosaccharides	10
Improving the catalytic efficiency of Pseudomonas aeruginosa lipoxygenase by semi-rational design	10
Efficient oxidation of 5-hydroxymethylfurfural to 2,5-furandicarboxylic acid by a two-enzyme system: Combination of a bacterial laccase with catalase	10
High stabilization and hyperactivation of a Recombinant β-Xylosidase through Immobilization Strategies	10
Identification of carotenoids biosynthesis pathway in Schizochytrium sp. and utilization in astaxanthin biosynthesis	10
Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene	10
High-level production of glucose oxidase in Pichia pastoris: Effects of Hac1p overexpression on cell physiology and enzyme expression	10
Caffeic acid production from glucose using metabolically engineered Escherichia coli	10
Enzymatic determination of hypoxanthine in fish samples as a freshness indicator using the CUPRAC colorimetric sensor	10
Moderate Electric Field Treatment Enhances Enzymatic Hydrolysis of Cellulose at Below-Optimal Temperatures	10
Enzymatic production of 3’-sialyllactose in milk	10
Combinational expression of D-sorbitol dehydrogenase and pyrroloquinoline quinone increases 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose production by Gluconobacter oxydans through cofactor mani	10
Synthesis of organic-inorganic hybrid nanoflowers of lipases from Candida antarctica type B (CALB) and Thermomyces lanuginosus (TLL): Improvement of thermal stability and reusability	10
Bio-specific immobilization of enzymes on electrospun PHB nanofibers	10
Enhanced catalytic efficiency and thermostability of glucose isomerase from Thermoanaerobacter ethanolicus via site-directed mutagenesis	10
Insights into extracellular dextran formation by Liquorilactobacillus nagelii TMW 1.1827 using secretomes obtained in the presence or absence of sucrose	10
Growth of ‘Candidatus Liberibacter asiaticus’ in a host-free microbial culture is associated with microbial community composition	10
Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique properties	10
Immobilization of bovine hemoglobin on Au nanoparticles/MoS2 nanosheets – Chitosan modified screen-printed electrode as chlorpyrifos biosensor	10
A lipase/poly (ionic liquid)-styrene microspheres/PVA composite hydrogel for esterification application	10
Enhanced removal of hydrocarbons BTX by light-driven Aspergillus niger ZnS nanobiohybrids	9
Enhancement of the water solubility and antioxidant capacities of mangiferin by transglucosylation using a cyclodextrin glycosyltransferase	9
Cloning, expression, biochemical characterization, and molecular docking studies of a novel glucose tolerant β-glucosidase from Saccharomonospora sp. NB11	9
Synthesis, characterization and immobilization of bilirubin oxidase nanoparticles (BOxNPs) with enhanced activity: Application for serum bilirubin determination in jaundice patients	9
Enzymatic synthesis of phlorizin fructosides	9
Characterization of a novel end product tolerant and thermostable cellulase from Neobacillus sedimentimangrovi UE25	9
A new FTIR assay for quantitative measurement of endo-fucoidanase activity	9
Enhancing the thermostability of a mono- and diacylglycerol lipase from Malassizia globose by stabilizing a flexible loop in the catalytic pocket	9
Impact of hydrophilic polymers in organosilica matrices on structure, stability, and biocatalytic activity of immobilized methylotrophic yeast used as biofilter bed	9
Preparation of a Bombyx mori acetylcholinesterase enzyme reagent through chaperone protein disulfide isomerase co-expression strategy in Pichia pastoris for detection of pesticides	9
Identification of a lactose-oxidizing enzyme in Escherichia coli and improvement of lactobionic acid production by recombinant expression of a quinoprotein glucose dehydrogenase from Pseudomonas taetr	9
Investigation of effect of ultrasound on immobilized C. rugosa lipase: Synthesis of biomass based furfuryl derivative and green metrics evaluation study	9
Enhancing the thermostability of D-allulose 3-epimerase from Clostridium cellulolyticum H10 via a dual-enzyme screening system	9
Immobilization of β-1,3-xylanase on pitch-based hyper-crosslinked polymers loaded with Ni2+ for algal biomass manipulation	9
Characterization of a thermostable PL-31 family alginate lyase from Paenibacillus ehimensis and its application for alginate oligosaccharides bioproduction	9
Fluorescence spectroscopy and molecular docking analysis of the binding of Lactobacillus acidophilus GIM1.208 β-glucosidase with quercetin glycosides	9
Divergent substrate specificities and regioselectivities of three lipase isoforms from Cordyceps militaris: Combinatorial advantages for entomopathogenicity and prospects as biocatalysts	9
One pot purification and co-immobilization of His-tagged old yellow enzyme and glucose dehydrogenase for asymmetric hydrogenation	9
Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity	9
Construction of novel curdlan-based and Ca2+-chelated magnetic microspheres (CCMM) for efficient protein purification and oriented immobilization	9
Biosynthesis of 3’-O-methylisoorientin from luteolin by selecting O-methylation/C-glycosylation motif	9
Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance	9