OOIR: Observatory of International Research

Papers

(The TQCC of Enzyme and Microbial Technology is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)

Article	Citations
Enhancing the thermal stability and activity of zearalenone lactone hydrolase to promote zearalenone degradation via semi-rational design	133
Carbohydrate-active enzyme-catalyzed stereoselective glycosylation of complex natural product glycosides	93
Osmoregulation by choline-based deep eutectic solvent induces electroactivity in Bacillus subtilis biofilms	83
Molecular cloning and characterization of a novel bifunctional cellobiohydrolase/β-xylosidase from a metagenomic library of mangrove soil	58
Analysis of enzyme kinetics of fungal methionine synthases in an optimized colorimetric microscale assay for measuring cobalamin-independent methionine synthase activity	46
Disrupting putative N-glycosylation site N17 in lipase Lip11 of Yarrowia lipolytica yielded a catalytically efficient and thermostable variant accompanying conformational changes	44
Microbial production of adipic acid from 6-hydroxyhexanoic acid for biocatalytic upcycling of polycaprolactone	39
More efficient barley malting under catalyst: Thermostability improvement of a β-1,3-1,4-glucanase through surface charge engineering with higher activity	38
Preparation of phytic acid modified α-Glucosidase/Cu3(PO4)2·3H2O hybrid nanoflower and its application	37
Enhancement of thermostability of GH10 xylanase E Penicillium canescens directed by ΔΔG calculations and structure analysis	35
Highly sensitive detection of glucose via glucose oxidase immobilization onto conducting polymer-coated composite polyacrylonitrile nanofibers	32
Latent potentials of the white-rot basidiomycete Phanerochaete chrysosporium responsible for sesquiterpene metabolism: CYP5158A1 and CYP5144C8 decorate (E)-α-bisabolene	31
Metataxonomic characterization of an autochthonous and allochthonous microbial consortium involved in a two-stage anaerobic batch reactor applied to hydrogen and methane production from sugarcane baga	30
Biochar production from microalgae: a new sustainable approach to wastewater treatment based on a circular economy	29
Insights on kraft lignin degradation in an anaerobic environment	27
Structures and properties of α-amylase and glucoamylase immobilized by ZIF-8 via one-pot preparation	26
A novel chondroitin AC lyase from Pedobacter xixiisoli: Cloning, expression, characterization and the application in the preparation of oligosaccharides	26
Structural and functional insights of the catalytic GH5 and Calx-β domains from the metagenome-derived endoglucanase CelE2	26
Microbial engineering for shikimate biosynthesis	26
Improving the cellobiose hydrolysis activity of glucose-stimulating β-glucosidase Bgl2A	25
Tailoring of levansucrase product size by a comparative molecular dynamics approach	25
Improvement of lipid production from glucose/xylose mixed-sugar by the oleaginous yeast Lipomyces starkeyi through ultra-violet mutagenesis	25
The potential of R. toruloides mevalonate pathway genes in increasing isoprenoid yields in S. cerevisiae: Evaluation of GGPPS and HMG-CoA reductase	25
Identification of a bacteria P450 enzyme from B. megaterium H-1 with vitamin D3 C-25 hydroxylation capabilities	24
Isolation of a newly Trichoderma asperellum LYS1 with abundant cellulase-hemicellulase enzyme cocktail for lignocellulosic biomass degradation	23

Cloning, expression, biochemical characterization, and molecular docking studies of a novel glucose tolerant β-glucosidase from Saccharomonospora sp. NB11	23
Development of a novel expression platform for heterologous protein production via deleting the p53-like regulator Vib1 in Trichoderma reesei	23
Production of a novel recombinant brown spider hyaluronidase in baculovirus-infected insect cells	23
Sensitivity of family GH11 Bacillus amyloliquefaciens xylanase A (BaxA) and the T33I mutant to Oryza sativa xylanase inhibitor protein (OsXIP): An experimental and computational study	23
Characterization and substrate-accelerated thermal inactivation kinetics of a new serine-type arylsulfatase	22
Cloning, purification and characterization of a novel thermostable recombinant tannase from Galactobacillus timonensis	22
Editorial Board	22
Identification of methionine adenosyltransferase with high diastereoselectivity for biocatalytic synthesis of (S)-S-adenosyl-l-methionine and exploring its relationship with fluorinated biosynthetic p	21
Nanobodies as binding-chaperones stabilize the recombinant Bombyx mori acetylcholinesterase and protect the enzyme activity in pesticide detection	21
Divergent substrate specificities and regioselectivities of three lipase isoforms from Cordyceps militaris: Combinatorial advantages for entomopathogenicity and prospects as biocatalysts	21
Editorial Board	21
Whole-cell biocatalyst for cadaverine production using stable, constitutive and high expression of lysine decarboxylase in recombinant Escherichia coli W3110	20
Development and expansion of the CRISPR/Cas9 toolboxes for powerful genome engineering in yeast	20
Efficient production of γ-aminobutyric acid using engineered Escherichia coli whole-cell catalyst	19
Rational design improves both thermostability and activity of a new D-tagatose 3-epimerase from Kroppenstedtia eburnean to produce D-allulose	19
Hyaluronic acid-coated chitosan nanoparticles as carrier for the enzyme/prodrug complex based on horseradish peroxidase/indole-3-acetic acid: Characterization and potential therapeutic for bladder can	19
Enzyme immobilization technology as a tool to innovate in the production of biofuels: A special review of the Cross-Linked Enzyme Aggregates (CLEAs) strategy	19
Robust cross-linked cyclodextrin glucanotransferase from Bacillus lehensis G1 aggregates using an improved cross-linker and a new co-aggregant for the production of cyclodextrins	19
Simultaneous enhancement of thermostability and catalytic activity of κ-carrageenase from Pseudoalteromonas tetraodonis by rational design	19
Efficient biosynthesis of lacto-N-neotetraose by a novel β-1,4-galactosyltransferase from Aggregatibacter actinomycetemcomitans NUM4039	19
Release of bifidogenic N-glycans from native bovine colostrum proteins by an endo-β-N-acetylglucosaminidase	18
Periplasmic expression of Pseudomonas fluorescens peroxidase Dyp1B and site-directed mutant Dyp1B enzymes enhances polymeric lignin degradation activity in Pseudomonas putida KT2440	18
Semi-rational engineering of ω-transaminase for enhanced enzymatic activity to 2-ketobutyrate	18
Mutational analysis of predicted active site residues of an exoglucanase secreted by Xanthomonas oryzae pv. oryzae to determine their role in catalysis and in virulence on rice	18
Editorial Board	17
Editorial Board	17
Design of a synthetic enzyme cascade for the in vitro fixation of formaldehyde to acetoin	17
Improving (2S)-naringenin production by exploring native precursor pathways and screening higher-active chalcone synthases from plants rich in flavonoids	16
High surfactant-tolerant β-mannanase isolated from Dynastes hercules larvae excrement, and identification of its hotspot using site-directed mutagenesis and molecular dynamics simulations	16
Improvements in xylose stability and thermalstability of GH39 β-xylosidase from Dictyoglomus thermophilum by site-directed mutagenesis and insights into its xylose tolerance mechanism	16
Production of recombinant glycosidases fused with Usp45 and SpaX to avoid the purification and immobilization stages	16
Editorial Board	15
Enhancement of rice husks saccharification through hydrolase preparation assisted by lytic polysaccharide monooxygenase	15
Editorial Board	15
Fluorescence spectroscopy and molecular docking analysis of the binding of Lactobacillus acidophilus GIM1.208 β-glucosidase with quercetin glycosides	15
Corrigendum to “Kinetic and thermodynamic study of laccase cross-linked onto glyoxyl Immobead 150P carrier: Characterization and application for beechwood biografting” [Enzym. Microb. Technol. 150 (20	15
Investigation of biocatalytic production of lactosucrose and fructooligosaccharides using levansucrases and dairy by-products as starting materials	14
Operation parameters and temperature affected sludge microbial metabolisms: An integrated perspective considering extracellular polymeric substances, soluble microbial products, biomass quantities, an	14
A glucose biosensor based on glucose oxidase fused to a carbohydrate binding module family 2 tag that specifically binds to the cellulose-modified electrode	14
A UPLC-based activity assay method for aminoglycoside phosphotransferase 4-Ia (APH4): A Selectable marker of plant transformation	14
Comparing similar versions of a connecting helix on the structure of Chondroitinase ABC I	14
Application of reductive amination by heterologously expressed Thermomicrobium roseum L-alanine dehydrogenase to synthesize L-alanine derivatives	14
Nanotechnology based technological development in biofuel production: Current status and future prospects	14
Improved thermostability of type I pullulanase from Bacillus thermoliquefaciens by error-prone PCR	14
Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli	14
Bioanalytical insight into the life of microbial populations: A chemical monitoring of ureolytic bacteria growth	14
Rational design of short-chain dehydrogenase DHDR for efficient synthesis of (S)-equol	14
Enzymatic determination of hypoxanthine in fish samples as a freshness indicator using the CUPRAC colorimetric sensor	14
Using large-scale multi-module NRPS to heterologously prepare highly efficient lipopeptide biosurfactants in recombinant Escherichia coli	14
Metabolism of phenolic compounds catalyzed by Tomato CYP736A61	13

Enzymatic production of chitooligosaccharides with high degree of polymerisations and their potential application to soy sauce preservation	13
Construction and characterization of a Myceliophthora thermophila lytic polysaccharide monooxygenase mutant S174C/A93C with improved thermostability	13
Cyanide-free synthesis of aromatic nitriles from aldoximes: Discovery and application of a novel heme-containing aldoxime dehydratase	13
Synthesis of lipophilic arbutin ester by enzymatic transesterification in high pressure carbon dioxide	13
Coriolus versicolor laccase-based inorganic protein hybrid synthesis for application in biomass saccharification to enhance biological production of hydrogen and ethanol	13
Protein engineering of an alkaline protease from Bacillus licheniformis (BLAP) for efficient and specific chiral resolution of the racemic ethyl tetrahydrofuroate	13
Targeted gene manipulation of Leloir pathway genes for the constitutive expression of β-galactosidase and its transgalactosylation product galacto-oligosaccharides from Kluyveromyces lactis GG799 and	13
Probiotic and functional characterization of newly isolated Lactiplantibacillus plantarum strains from human breast milk and proliferative inhibition potential of metabolites	13
De novo biosynthesis of tyrosol acetate and hydroxytyrosol acetate from glucose in engineered Escherichia coli	13
Double T-junction microfluidic and conventional dripping systems for Bacillus subtilis immobilization in calcium alginate microparticles for lipase production	13
Enhanced biotransformation of the minor ginsenosides in red ginseng extract by Penicillium decumbens β-glucosidase	13
Metagenomic insight on consortium degradation of soil weathered petroleum and its supplement based on gene abundance change	13
Non-covalent and covalent immobilization of papain onto Ti3C2 MXene nanosheets	13
A simple and green method for direct determination of hydrogen peroxide and hypochlorite in household disinfectants based on personal glucose meter	13
Biocatalytic valorization of lignin subunit: Screening a carboxylic acid reductase with high substrate preference to syringyl functional group	13
Penicillin binding proteins-based immunoassay for the selective and quantitative determination of beta-lactam antibiotics	12
Screening β-glucosidase and α-rhamnosidase for biotransformation of naringin to naringenin by the one-pot enzymatic cascade	12
Functional characterization of a GH62 family α-L-arabinofuranosidase from Eupenicillium parvum suitable for monosaccharification of corncob arabinoxylan in combination with key enzymes	12
Unveiling steps of the TDP degradation pathway in Variovorax paradoxus TBEA6	12
Biotransformation of ferulic acid into vanillyl alcohol and vanillic acid employing thermophilic bacterium Caldimonas thermodepolymerans	12
Agmatine production by Escherichia coli cells expressing SpeA on the extracellular surface	12
Development of a chloroplast expression system for Dunaliella salina	12
Catalytically active inclusion bodies (CatIBs) induced by terminally attached self-assembling coiled-coil domains: To enhance the stability of (R)-hydroxynitrile lyase	12
Immobilization of Thermomyces lanuginosus lipase on a new hydrophobic support (Streamline phenyl™): Strategies to improve stability and reusability	12
Editorial Board	12
Continuous production of gamma aminobutyric acid by engineered and immobilized Escherichia coli whole-cells in a small-scale reactor system	12
Editorial Board	12
Action pattern of Sulfolobus O-α-glycoligase for synthesis of highly water soluble resveratrol 3,4′-α-diglucoside	12
Isolation of new lactobionic acid-producing microorganisms and improvement of their production ability by heterologous expression of glucose dehydrogenase from Pseudomonas taetrolens	12
Editorial Board	12
Editorial Board	12
Enhanced isomerization of rare sugars by ribose-5-phosphate isomerase A from Ochrobactrum sp. CSL1	11
The increased efficiency of porphyran hydrolysis by constructing a multifunctional enzyme complex from marine microorganisms	11
Efficient snailase-based production of mogrol from Luo Han Guo extract in an aqueous-organic system	11
Disruption of vacuolar protein sorting receptor gene Poxvps10 improves cellulolytic enzyme production by Penicillium oxalicum	11
Carbohydrate-binding modules of ChiB and ChiC promote the chitinolytic system of Serratia marcescens BWL1001	11
Ternary biogenic silica/magnetite/graphene oxide composite for the hyperactivation of Candida rugosa lipase in the esterification production of ethyl valerate	11
CO2 capture and lipid production performance of microalgae in the S-shaped photobioreactor under different culture modes	11
Whole-cell bioconversion for producing thymoquinone by engineered Saccharomyces cerevisiae	11
Efficient production of isoquercitin, icariin and icariside II by a novel thermostable α-l-rhamnosidase PodoRha from Paenibacillus odorifer with high α-1, 6-/α-1, 2- glycoside specificity	11
Co-expression of an isopropanol synthetic operon and eGFP to monitor the robustness of Cupriavidus necator during isopropanol production	11
l-tryptophan-histidine synthesis by Pseudomonas serine peptidase, an amino acid ester hydrolase of the peptidase family S9	11
A facile and one-pot aqueous phase transfer of oleylamine capped Au NP with aminophenylboronic acid used as transfer and targeting ligand	11
Mutant β-fructofuranosidase synthesizing blastose [β-d-Fruf-(2→6)-d-Glcp]	11
Biochemical characterisation of glycosylated and deglycosylated forms of phytase from Cronobacter turicensis expressed in Pichia pastoris	11
Utilization of sweet sorghum juice as a carbon source for enhancement of itaconic acid production in engineered Corynebacterium glutamicum	11
A green pathway for lignin valorization: Enzymatic lignin depolymerization in biocompatible ionic liquids and deep eutectic solvents	10
One pot purification and co-immobilization of His-tagged old yellow enzyme and glucose dehydrogenase for asymmetric hydrogenation	10
Impact of hydrophilic polymers in organosilica matrices on structure, stability, and biocatalytic activity of immobilized methylotrophic yeast used as biofilter bed	10
A thermophilic phosphatase from Methanothermobacter marburgensis and its application to in vitro biosynthesis	10
Editorial Board	10
Identification of carotenoids biosynthesis pathway in Schizochytrium sp. and utilization in astaxanthin biosynthesis	10
Synergistic action of thermophilic pectinases for pectin bioconversion into D-galacturonic acid	10
Cellulolytic enzymes production guided by morphology engineering	10
Heterologous expression, purification, and characterization of a recombinant Cordyceps militaris lipase from Candida rugosa-like family in Pichia pastoris	10
Full-length TprK of Treponema pallidum subsp. pallidum in lipid nanodiscs is a monomeric porin	10
Non-covalent binding tags for batch and flow biocatalysis	10
A new continuous assay for quantitative assessment of enzymatic degradation of poly(ethylene terephthalate) (PET)	10
Ancestral sequence reconstruction and spatial structure analysis guided alteration of longer-chain substrate catalysis for Thermomicrobium roseum lipase	10
Editorial Board	10
Using directed evolution to improve hydrogen production in chimeric hydrogenases from algal species	10
Production and characterization of novel thermostable CotA-laccase from Bacillus altitudinis SL7 and its application for lignin degradation	9
Production of an O-desmethylated product, a major human metabolite, of rabeprazole sulfide by bacterial P450 enzymes	9
Poly(3-hydroxybutyrate) production for food packaging from biomass derived carbohydrates by cupriavidus necator DSM 545	9
Immobilization on magnetic PVA/SA@Fe3O4 hydrogel beads enhances the activity and stability of neutral protease	9
Applying molecular and phenotypic screening assays to identify efficient quorum quenching lactonases	9
Engineering a ComA Quorum-Sensing circuit to dynamically control the production of Menaquinone-4 in Bacillus subtilis	9
A GH19 lysozyme and peptidase from Myoviridae cyanophages lacking the typical holin-endolysin system exhibit lytic activity	9
Identification of 2’-deoxyguanosine for an α-amylase inhibitor in the extracts of the earthworm Eisenia fetida and characterization of its inhibitory activity against porcine pancreatic α-amylase	9
A study of targeted mutation of l-rhamnose isomerase to improve the conversion efficiency of D-allose	9
Editorial Board	9
Synthesis of improved long-chain isomaltooligosaccharide, using a novel glucosyltransferase derived from Thermoanaerobacter thermocopriae, with maltodextrin	9
Decyl esters production from soybean-based oils catalyzed by lipase immobilized on differently functionalized rice husk silica and their characterization as potential biolubricants	9
Editorial Board	9
Autodisplay of an endo-1,4-β-xylanase from Clostridium cellulovorans in Escherichia coli for xylans degradation	9
Cell-free production of the bifunctional glycoside hydrolase GH78 from Xylaria polymorpha	9
Improvement in salt-tolerance of Aspergillus oryzae γ-glutamyl transpeptidase via protein chimerization with Aspergillus sydowii homolog	9
Cross-linking of carbonic anhydrase and formate dehydrogenase based on amino acid specific recognition: Conversion of carbon dioxide to formic acid	9
Engineered Zea mays phenylalanine ammonia-lyase for improve the catalytic efficiency of biosynthesis trans-cinnamic acid and p-coumaric acid	9