OOIR: Observatory of International Research

Papers

(The TQCC of Journal of Immunological Methods is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-11-01 to 2024-11-01.)

Article	Citations
Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples	63
Methodological comparison of FACS and MACS isolation of enriched microglia and astrocytes from mouse brain	63
Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria	50
Lymphocyte transformation test: History and current approaches	42
Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay	36
Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients	34
A novel co-culture assay to assess anti-tumor CD8+ T cell cytotoxicity via luminescence and multicolor flow cytometry	32
Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies	31
Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples	26
SARS-CoV-2 serology testing: Progress and challenges	25
Enrichment of circulating tumor-derived extracellular vesicles from human plasma	22
An evaluation of sorter induced cell stress (SICS) on peripheral blood mononuclear cells (PBMCs) after different sort conditions - Are your sorted cells getting SICS?	21
Development and validation of ELISAs for the quantitation of interleukin (IL)-1β, IL-6, IL-8 and IL-10 in ovine plasma	19
Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects	18
Multiplex agglutination-PCR (ADAP) autoantibody assays compared to radiobinding autoantibodies in type 1 diabetes and celiac disease	18
A streamlined proliferation assay using mixed lymphocytes for evaluation of human mesenchymal stem cell immunomodulation activity	17
SARS-CoV-2 variants lacking a functional ORF8 may reduce accuracy of serological testing	16
Basophil and mast cell activation tests by flow cytometry in immediate drug hypersensitivity: Diagnosis and beyond	16
Detection of recent infection of Japanese encephalitis virus in swine population using IgM ELISA: A suitable sentinel to predict infection in humans	16
Qualification of ELISA and neutralization methodologies to measure SARS-CoV-2 humoral immunity using human clinical samples	15
ELISpot assay as a diagnostic tool in drug hypersensitivity reactions	14
A method for expansion and retroviral transduction of mouse regulatory T cells	14
Analysis of COVID-19 convalescent plasma for SARS-CoV-2 IgG using two commercial immunoassays	14
Quantification of trivalent non-replicating rotavirus vaccine antigens in the presence of aluminum adjuvant	13
The spike-ACE2 binding assay: An in vitro platform for evaluating vaccination efficacy and for screening SARS-CoV-2 inhibitors and neutralizing antibodies	13

Cross-platform comparison of immune-related gene expression to assess intratumor immune responses following cancer immunotherapy	13
Antinuclear antibodies (ANAs) detected by indirect immunofluorescence (IIF) method in acute COVID-19 infection; future roadmap for laboratory diagnosis	13
Operational characteristics of 30 lateral flow immunoassays used to identify COVID-19 immune response	13
Lymphocyte transformation test and cytokine detection assays: Determination of read out parameters for delayed-type drug hypersensitivity reactions	13
Monitoring Ravulizumab effect on complement assays	12
Guidelines for analysis of low-frequency antigen-specific T cell results: Dye-based proliferation assay vs 3H-thymidine incorporation	12
Surface Plasmon Resonance (SPR) biosensor for detection of mycotoxins: A review	12
An optimized flow cytometry panel for classifying macrophage polarization	12
Immunogenicity evaluating of the SLNs-alginate conjugate against Pseudomonas aeruginosa	12
Characterization of a rat monoclonal antibody raised against ferroptotic cells	12
Complement activation in human autoimmune diseases and mouse models; employing a sandwich immunoassay specific for C3dg	12
Generation of a cost-effective cell line for support of high-throughput isolation of primary human B cells and monoclonal neutralizing antibodies	12
Effects of long-term cryopreservation of PBMC on recovery of B cell subpopulations	11
The antinuclear antibody dense fine speckled pattern and possible clinical associations: An indication of a proinflammatory microenvironment	11
Development of magnetic particle-based chemiluminescence immunoassay for measurement of human procalcitonin in serum	11
SCHNAPPs - Single Cell sHiNy APPlication(s)	11
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19	11
Peripheral blood cultured mast cells: Phenotypic and functional outcomes of different culture protocols	10
An eight-plex immunoassay for Group A streptococcus serology and vaccine development	10
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	10
Comprehensive epitope mapping using polyclonally expanded human CD8 T cells and a two-step ELISpot assay for testing large peptide libraries	10
Multiplex immunoassay to measure antibody response to nine HPV vaccine types	10
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions	10
Diagnostic and analytical performance evaluation of ten commercial assays for detecting SARS-CoV-2 humoral immune response	10
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	10
Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay	10
Development of a time-resolved fluorescence immunoassay based on immunomagnetic beads for gastrin-17	9
Multiplex bead binding assays using off-the-shelf components and common flow cytometers	9
Clinical and analytical evaluation of the Abbott AdviseDx quantitative SARS-CoV-2 IgG assay and comparison with two other serological tests	9
Estimation of ELISA results using a parallel curve analysis	9
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination	9
An optimized flow cytometry protocol for simultaneous detection of T cell activation induced markers and intracellular cytokines: Application to SARS-CoV-2 immune individuals	9
Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis	9
The role of antibody-based troponin detection in cardiovascular disease: A critical assessment	9
A flow cytometry-based proliferation assay for clinical evaluation of T-cell memory against SARS-CoV-2	8
Culturing cells with mast cell phenotype and function: Comparison of peripheral blood and bone marrow as a source.	8
Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination	8
Feasibility of using monocyte-derived dendritic cells obtained from cryopreserved cells for DC-based vaccines	8
Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis	8
A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensor	8
Novel immunoassay for diagnosis of ongoing Clostridioides difficile infections using serum and medium enriched for newly synthesized antibodies (MENSA)	8
A high-throughput multiplex assay to characterize flavivirus-specific immunoglobulins	8
Identification of tumor-associated antigens of lung cancer: SEREX combined with bioinformatics analysis	8
Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID19	8
A novel enzyme immunoassay for the measurement of plasma (1 → 3)-β-D-glucan levels	7
Isolation and culture of rat intestinal mucosal microvascular endothelial cells using immunomagnetic beads	7
Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of sows	7
Sandwich-type immunosensor based on COF-LZU1 as the substrate platform and graphene framework supported nanosilver as probe for CA125 detection	7
A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system	7
Optimized flow cytometry assays to monitor neutrophil activation in human and mouse whole blood samples	7

Detergent wash improves vaccinated lymph node handling ex vivo	7
Allergen extraction: Factors influencing immunogenicity and sensitivity of immunoassays	7
Cryopreservation of peripheral blood mononuclear cells for use in proliferation assays: First step towards potency assays	7
Future perspectives on in-vitro diagnosis of drug allergy by the lymphocyte transformation test	7
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species	7
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	6
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution	6
Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses	6
Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies	6
Production and characterization of novel monoclonal antibodies against outer membrane protein A (OmpA) of Acinetobacter baumannii	6
The crux of C1-INH testing in everyday lab work	6
A sensitive method to quantify HIV-1 antibodies in mucosal samples	6
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection	6
Urinary cell mRNA profiling of kidney allograft recipients: Development of a portable protocol for noninvasive diagnosis of T cell mediated rejection and BK virus nephropathy	6
Development and optimization of a Zika virus antibody-dependent cell-mediated cytotoxicity (ADCC) assay	6
Non-glycosylated SARS-CoV-2 RBD elicited a robust neutralizing antibody response in mice	6
Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy	6
Small p53 derived peptide suitable for robust nanobodies dimerization	6
An in vitro refolding method to produce oligomers of anti-CHIKV, E2-IgM Fc fusion subunit vaccine candidates expressed in E. coli	6
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses	6
Brucella antigens (BhuA, 7α-HSDH, FliC) in poly I:C adjuvant as potential vaccine candidates against brucellosis	6
Novel bridge multi-species ELISA for detection of SARS-CoV-2 antibodies	6
Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus	6
Development of a non-biased, high-throughput ELISA for the rapid evaluation of immunogenicity and cross-reactivity	5
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	5
Murine monoclonal antibodies against RBD of the SARS-CoV-2 spike protein as useful analytical tools for subunit vaccine development and clinical trials	5
A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells	5
A multifaceted high-throughput assay for probing antigen-specific antibody-mediated primary monocyte phagocytosis and downstream functions	5
Impact of complement and difference of cell-based assay and ELISA in determination of neutralization capacity against mumps and measles virus	5
Production, characterization and in-vitro applications of single-domain antibody against thyroglobulin selected from novel T7 phage display library	5
Relationship between circulating tumor-associated autoantibodies and clinical outcomes in advanced-stage NSCLC patients receiving PD-1/−L1 directed immune checkpoint inhibition.	5
Development and qualification of a fast, high-throughput and robust imaging-based neutralization assay for respiratory syncytial virus	5
An innovative method for characterizing neutralizing antibodies against antibody-derived therapeutics	5
Positive tissue transglutaminase antibodies with negative endomysial antibodies: Unresolved issues in diagnosing celiac disease	5
Performance of a commercially available multiplex platform in the assessment of circulating cytokines and chemokines in patients with rheumatoid arthritis and osteoarthritis	5
Gene knockout in highly purified mouse hematopoietic stem cells by CRISPR/Cas9 technology	5
A light-initiated chemiluminescent assay for the detection of children's milk protein-specific IgE with excellent ability to avoid interference of specific IgG	5
Comparison of different immunoassays for the detection of antibodies against Intrinsic Factor and Parietal Cells	5
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood	5
Development of competitive inhibition ELISA as an effective potency test to analyze human rabies vaccines and assessment of the antigenic epitope of rabies glycoprotein	5
Development of a bead-based multiplex immunoassay for simultaneous quantitative detection of IgG serum antibodies against seven vaccine-preventable diseases	5
In silico engineering a CD80 variant with increased affinity to CTLA-4 and decreased affinity to CD28 for optimized cancer immunotherapy	5
In silico designed Staphylococcus aureus B-cell multi-epitope vaccine did not elicit antibodies against target antigens suggesting multi-domain approach	5
Development and validation of a novel immunogenicity assay to detect anti-drug and anti-PEG antibodies simultaneously with high sensitivity	5
TIL expansion with high dose IL-2 or low dose IL-2 with anti-CD3/anti-CD28 stimulation provides different quality of TIL-expanded T cell clones	5
Acquired B-cell deficiency secondary to B-cell-depleting therapies	5
A multi-colour confocal microscopy method for identifying and enumerating macrophage subtypes and adherent cells in the stromal vascular fraction of human adipose	4
Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay	4
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma	4
Bacterial coloration immunofluorescence strip for ultrasensitive rapid detection of bacterial antibodies and targeted antibody-secreting hybridomas	4
Overview of human B-cell development and antibody deficiencies	4
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A	4
Development of a Bak gene based standard curve for absolute quantification of BK virus in real time quantitative PCR assay and noninvasive diagnosis of BK virus nephropathy in kidney allograft recipie	4
Broad immunophenotyping of the murine brain tumor microenvironment	4
Rapid immunomagnetic co-capture assay for quantification of lung cancer associated exosomes	4
Tracking fluorescently labeled IL-15 and anti-PD-1 in the tumor microenvironment and draining lymph nodes	4
A 33-color panel of phenotypic analysis of murine organ specific immune cells	4
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays	4
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies	4
Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination	4
Mapping antibody binding using multiplexed epitope substitution analysis	4
Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants	4
Flow cytometry-based immunophenotyping of myeloid-derived suppressor cells in human breast cancer patient blood samples	4
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen	4
Development of a new peptide-bead coupling method for an all peptide–based Luminex multiplexing assay for detection of Plasmodium falciparum antibody responses	4
High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design	4
Blood biomarker algorithms for the diagnosis of mycoplasma pneumoniae respiratory infections	4
Clinical evaluation of commercial SARS-CoV-2 serological assays in a malaria endemic setting	4
Detection of engineered T cells in FFPE tissue by multiplex in situ hybridization and immunohistochemistry	4
Evaluation of antibody titer kinetics and SARS-CoV-2 infections in a large cohort of healthcare professionals ten months after administration of the BNT162b2 vaccine	4
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors	4
Objective evaluation of immediate reading skin prick test applying image planimetric and reaction thermometry analyses	4
Evaluation of ELISA and VNT for sheeppox virus antibody detection and development of an immunoenzymatic quantitative method	4
Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?	4
Impact of delayed PBMC processing on functional and genomic assays	4
Urinary cell mRNA profiling of kidney allograft recipients: A systematic investigation of a filtration based protocol for the simplification of urine processing	4
Distinct and overlapping effects of β2-glycoprotein I conformational variants in ligand interactions and functional assays	4