Journal of Immunological Methods

Article	Citations
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells	63
Mapping antibody binding using multiplexed epitope substitution analysis	50
Editorial Board	42
Editorial Board	39
Non-invasive image-based cytometry for high throughput NK cell cytolysis analysis	34
A chimeric HLA-A2:β2M:Ig fusion protein for the study of virus-specific CD8+ T-cells	31
Development of a mechanism of action reflective and robust potency assay for a therapeutic antibody against alpha toxin using rabbit erythrocytes	26
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species	25
Development of ELISA for the detection of antibodies against VP2 protein of bluetongue virus serotype-1	22
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis	18
Editorial Board	18
A novel method for in vitro culture and expansion of nonhuman primate B cells	17
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring	16
Editorial Board	16
Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants	16
Construction of a full-length antibody phage display vector	15
Establishment of a homogeneous immunoassay-light-initiated chemiluminescence assay for detecting anti-Müllerian hormone in human serum	14
Immunoglobulin isotype compositions of ABO specific antibodies are dependent on the individual patient blood group and blood group specificity: Results from a healthy donor cohort	14
Establishment of a novel cell-based assay using HLA-transfected cells to detect HLA antibodies	13
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection	13
A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue	13
Role of antinuclear antibodies in COVID-19 patients	13
Editorial Board	13
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute	13
Characterization and ex vivo expansion of rare in situ cytokine secreting T cell populations from tumor tissue and blood of oral squamous cell carcinoma patients	13

Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses	12
A single homogeneous assay for simultaneous measurement of bispecific antibody target binding	12
Development of rELISA using novel markers for the diagnosis of paratuberculosis	12
Optimized cytotoxicity assay for co-suspended effector and target cells	12
Development of a new automated IL-6 immunoassay	11
Immunoassay for detection of oligomeric proteins	11
Editorial Board	11
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer	11
Thanking the reviewers of Journal of Immunological Methods	11
Critical evaluation of fluorescent dyes to evaluate the stability and ligand binding properties of an anti-cocaine mAb, h2E2	11
An enzyme-free method for isolating testicular macrophages from rodent models	10
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia	10
Editorial Board	10
Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response	10
Editorial Board	10
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes	10
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	10
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays	10
Editorial introduction for “B cell immunodeficiencies” Journal of Immunological Methods special edition	10
Development of competitive inhibition ELISA as an effective potency test to analyze human rabies vaccines and assessment of the antigenic epitope of rabies glycoprotein	9
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis	9
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	9
SARS-CoV-2 variants lacking a functional ORF8 may reduce accuracy of serological testing	9
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing	9
Digital RT-PCR Chip method for detection of SARS-CoV-2 virus	9
Quantitative determination of C-polysaccharide in Streptococcus pneumoniae capsular polysaccharides by enzyme-linked immunosorbent assay	9
Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies	8
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue	8
Development of gold nanocluster complex for the detection of tumor necrosis factor-alpha based on immunoassay	8
Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples	8
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults	8
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood	8
Comparison of single-chain variable fragments and monoclonal antibody against dihydroartemisinin	8
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine	8
Comparison of hemagglutination inhibition tests, immunoperoxidase monolayer assays, and serum neutralizing tests in detecting antibodies against blue eye disease in pigs	8
Selection of positive controls and their impact on anti-drug antibody assay performance	8
Development of a flow cytometry-based potency assay for prediction of cytokine storms induced by biosimilar monoclonal antibodies	7
A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells	7
Immunodetection of Furcraea Necrotic Streak Virus-FNSV in fique plants (Furcraea macrophylla Baker) using a polyclonal antibody IgY produced in chicken egg yolk	7
Characterization of a rat monoclonal antibody raised against ferroptotic cells	7
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction	7
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution	7
Development of immunochromatographic strip assay to detect recent infection of Japanese encephalitis virus in swine population	7
A sensitive method to quantify HIV-1 antibodies in mucosal samples	7
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions	7
Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies	6
Overview of human B-cell development and antibody deficiencies	6
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials	6
Smartphone based lateral flow immunoassay quantifications	6
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp	6

Agreement and error of titration assays	6
Feasibility of using monocyte-derived dendritic cells obtained from cryopreserved cells for DC-based vaccines	6
Development of an indirect competitive enzyme linked immunosorbent assay for the quantitative detection of Mycoplasma hyopneumoniae during the vaccine production process	6
Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay	6
Candidate antibody reference reagents for Chlamydia trachomatis serology	6
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells	6
Development of an ELISA for an effective potency determination of recombinant rabies human monoclonal antibody	6
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA242	6
Parameters of immunoglobulin extraction from dried blood spot cards and immunoassays for detection of antibody response to pathogens including the novel SARS-CoV-2	6
Small p53 derived peptide suitable for robust nanobodies dimerization	6
Editorial – Immunobiophysics: Advances and techniques	6
Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay	6
Editorial Board	5
Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients	5
Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay	5
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	5
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides	5
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus	5
Development of a light-initiated chemiluminescence assay for the quantitative detection of cow's milk allergen-specific IgE	5
Bacterial coloration immunofluorescence strip for ultrasensitive rapid detection of bacterial antibodies and targeted antibody-secreting hybridomas	5
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids	5
Epitope profiling of cow's milk allergen-specific antibodies with determining IgE content in epitopes-ALL, a 14-epitopes mixture	5
Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID19	5
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library	5
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors	5
Dissociation of murine oral mucosal tissues for single cell applications	5
Multiplex bead binding assays using off-the-shelf components and common flow cytometers	5
Surface Plasmon Resonance (SPR) biosensor for detection of mycotoxins: A review	5
Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of sows	5
Isolation of phage-antibodies against Eimeria species that infect chickens	5
Fine definition of the epitopes on the human gp91 /NOX2 for the monoclonal antibodies CL-5 and 48	5
Novel immunoassay for diagnosis of ongoing Clostridioides difficile infections using serum and medium enriched for newly synthesized antibodies (MENSA)	5
Validation of dried blood spots samples for the screening of hepatitis B virus surface by enzyme immunoassay method	5
Letter to the editor regarding “Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials”	5
An easy assay to detect autoantibodies neutralizing cytokines in subjects with critical infections	5
In vitro immunization approach to generate specific murine monoclonal IgG antibodies	4
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay	4
Ultrasensitive multiplexed chemiluminescent enzyme-linked immunosorbent assays in 384-well plates	4
Editorial Board	4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response	4
Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep	4
Potential diagnostic application of the baculovirus-expressed recombinant truncated nucleocapsid protein of peste des petits ruminants virus in ELISA	4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay	4
Assessing TCR identity, knock-in efficiency, and potency for individualized TCR-T cell therapy	4
Editorial Board	4
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing	4
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice	4
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies	4
Generation of good manufacturing practice grade virus-specific T cells for the management of post-transplant CMV infections	4
A method for expansion and retroviral transduction of mouse regulatory T cells	4
Comprehensive phenotyping of circulating immune cell subsets in people living with HIV	4
Stability of anti-drug antibodies in human serum samples	4
Editorial Board	4
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma	4
Evaluation of antibody titer kinetics and SARS-CoV-2 infections in a large cohort of healthcare professionals ten months after administration of the BNT162b2 vaccine	4
Flow cytometry for B-cell subset analysis in immunodeficiencies	4
Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination	4
Monocyte monolayer assay in pre-transfusion testing: A magic key in transfusing patients with recurrent bad cross-match due to alloimmunization	4
Reply to “Precision-cut human liver slice cultures as an immunological platform”	4
In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-1	4
Novel hapten design, highly sensitive monoclonal antibody production, and immunoassay development for rapid screening of illegally added chloramphenicol in cosmetics	4
Flow cytometric determination of ferroptosis using a rat monoclonal antibody raised against ferroptotic cells	3
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a	3
Comparative assessment of cytometry by time-of-flight and full spectral flow cytometry based on a 33-color antibody panel	3
Development of a non-biased, high-throughput ELISA for the rapid evaluation of immunogenicity and cross-reactivity	3
Establishment of bioluminescent imaging model using murine T cell lymphoma susceptive to NK cell-dependent immune-surveillance	3
Development and qualification of a fast, high-throughput and robust imaging-based neutralization assay for respiratory syncytial virus	3
Highlights of the 33rd annual scientific meeting of the Association of Medical Laboratory Immunologists (AMLI)	3
Detection of alloreactive T cells from cryopreserved human peripheral blood mononuclear cells	3
High-yield production of recombinant human myelin oligodendrocyte glycoprotein in SHuffle bacteria without a refolding step	3
Comparative analysis of LAG3 antibodies shows differential binding patterns by flow cytometry	3
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	3
Generation of a cost-effective cell line for support of high-throughput isolation of primary human B cells and monoclonal neutralizing antibodies	3
Detection of RNA-dependent RNA polymerase of porcine epidemic diarrhea virus	3
Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles	3
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols	3
Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination	3
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation	3
ERAMER: A novel in silico tool for prediction of ERAP1 enzyme trimming	3
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance	3

Immunofluorescence analysis of human eosinophils	3
Editorial Board	3
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies	3
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure	3
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments	3
Mitochondrial function in oral health and disease	3
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood	3
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood	3
An eight-plex immunoassay for Group A streptococcus serology and vaccine development	3
Retention of E-selectin functionalized liposome fanny packs on Jurkat cells following invasion through collagen	3
Editorial Board	3
Development and evaluation of a multi-antigen serological assay for the intra-vitam diagnosis of Tuberculosis caused by Mycobacterium bovis in pigs	3
The C-terminus of Brucella abortus MviN induces humoral and cell mediated immune responses in BALB/c mice that protects against the virulent Brucella 544 challenge	3
Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cells	3
Editorial – Special Issue on in vitro detection of drug allergy	3
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry	3
Detergent wash improves vaccinated lymph node handling ex vivo	3
A novel whole blood assay to quantify the release of T cell associated cytokines in response to Bordetella pertussis antigens	3
A novel antigen immunochromatography fluorometric strip for rapid detection and application of pathogenic bacterial high-quality antibody	3
SARS-CoV-2 serology testing: Progress and challenges	3
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?	3
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19	3