OOIR: Observatory of International Research

Papers

(The TQCC of Journal of Immunological Methods is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-04-01 to 2024-04-01.)

Article	Citations
SARS-CoV-2-specific ELISA development	69
Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples	62
Methodological comparison of FACS and MACS isolation of enriched microglia and astrocytes from mouse brain	54
Lymphocyte transformation test: History and current approaches	37
Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria	37
openPrimeR for multiplex amplification of highly diverse templates	33
Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients	33
Normal human lymph node T follicular helper cells and germinal center B cells accessed via fine needle aspirations	32
Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies	29
Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay	28
Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples	26
A novel co-culture assay to assess anti-tumor CD8+ T cell cytotoxicity via luminescence and multicolor flow cytometry	24
T follicular helper and T follicular regulatory cells in colorectal cancer: A complex interplay	23
SARS-CoV-2 serology testing: Progress and challenges	21
Enrichment of circulating tumor-derived extracellular vesicles from human plasma	19
An evaluation of sorter induced cell stress (SICS) on peripheral blood mononuclear cells (PBMCs) after different sort conditions - Are your sorted cells getting SICS?	18
Development and validation of ELISAs for the quantitation of interleukin (IL)-1β, IL-6, IL-8 and IL-10 in ovine plasma	17
Rational application of nanoadjuvant for mucosal vaccine delivery system	16
SARS-CoV-2 variants lacking a functional ORF8 may reduce accuracy of serological testing	16
Clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies testing	15
Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects	15
A streamlined proliferation assay using mixed lymphocytes for evaluation of human mesenchymal stem cell immunomodulation activity	14
Analysis of COVID-19 convalescent plasma for SARS-CoV-2 IgG using two commercial immunoassays	14
Basophil and mast cell activation tests by flow cytometry in immediate drug hypersensitivity: Diagnosis and beyond	14
Development of a novel chemiluminescence immunoassay for the detection of procalcitonin	13

Antinuclear antibodies (ANAs) detected by indirect immunofluorescence (IIF) method in acute COVID-19 infection; future roadmap for laboratory diagnosis	13
Cross-platform comparison of immune-related gene expression to assess intratumor immune responses following cancer immunotherapy	13
A method for expansion and retroviral transduction of mouse regulatory T cells	13
Lymphocyte transformation test and cytokine detection assays: Determination of read out parameters for delayed-type drug hypersensitivity reactions	13
Implementation of a three-tiered approach to identify and characterize anti-drug antibodies raised against HIV-specific broadly neutralizing antibodies	12
Operational characteristics of 30 lateral flow immunoassays used to identify COVID-19 immune response	12
Immunogenicity evaluating of the SLNs-alginate conjugate against Pseudomonas aeruginosa	12
Quantification of trivalent non-replicating rotavirus vaccine antigens in the presence of aluminum adjuvant	12
Detection of recent infection of Japanese encephalitis virus in swine population using IgM ELISA: A suitable sentinel to predict infection in humans	12
Characterization of a rat monoclonal antibody raised against ferroptotic cells	11
The spike-ACE2 binding assay: An in vitro platform for evaluating vaccination efficacy and for screening SARS-CoV-2 inhibitors and neutralizing antibodies	11
ELISpot assay as a diagnostic tool in drug hypersensitivity reactions	11
Qualification of ELISA and neutralization methodologies to measure SARS-CoV-2 humoral immunity using human clinical samples	11
Guidelines for analysis of low-frequency antigen-specific T cell results: Dye-based proliferation assay vs 3H-thymidine incorporation	11
Multiplex agglutination-PCR (ADAP) autoantibody assays compared to radiobinding autoantibodies in type 1 diabetes and celiac disease	11
A novel efficient bispecific antibody format, combining a conventional antigen-binding fragment with a single domain antibody, avoids potential heavy-light chain mis-pairing	10
Effects of long-term cryopreservation of PBMC on recovery of B cell subpopulations	10
The antinuclear antibody dense fine speckled pattern and possible clinical associations: An indication of a proinflammatory microenvironment	10
Diagnostic and analytical performance evaluation of ten commercial assays for detecting SARS-CoV-2 humoral immune response	10
Monitoring Ravulizumab effect on complement assays	10
Complement activation in human autoimmune diseases and mouse models; employing a sandwich immunoassay specific for C3dg	10
Peripheral blood cultured mast cells: Phenotypic and functional outcomes of different culture protocols	9
Generation of a cost-effective cell line for support of high-throughput isolation of primary human B cells and monoclonal neutralizing antibodies	9
Development of magnetic particle-based chemiluminescence immunoassay for measurement of human procalcitonin in serum	9
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	9
In the search of potential epitopes for Wuhan seafood market pneumonia virus using high order nullomers	9
Optimization and qualification of a functional anti-drug antibody assay for HIV-1 bnAbs	9
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions	9
Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis	8
Estimation of ELISA results using a parallel curve analysis	8
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-19	8
SCHNAPPs - Single Cell sHiNy APPlication(s)	8
Identification of tumor-associated antigens of lung cancer: SEREX combined with bioinformatics analysis	8
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	8
A flow cytometry-based proliferation assay for clinical evaluation of T-cell memory against SARS-CoV-2	8
Comprehensive epitope mapping using polyclonally expanded human CD8 T cells and a two-step ELISpot assay for testing large peptide libraries	8
Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID19	8
Future perspectives on in-vitro diagnosis of drug allergy by the lymphocyte transformation test	7
Multiplex bead binding assays using off-the-shelf components and common flow cytometers	7
A high-throughput multiplex assay to characterize flavivirus-specific immunoglobulins	7
Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells	7
Agrodiag PorCoV: A multiplex immunoassay for the differential diagnosis of porcine enteric coronaviruses	7
Importance of validating antibody panels: Anti-PD-L1 clone binds AF700 fluorophore	7
The role of antibody-based troponin detection in cardiovascular disease: A critical assessment	7
A novel enzyme immunoassay for the measurement of plasma (1 → 3)-β-D-glucan levels	7
Multiplex immunoassay to measure antibody response to nine HPV vaccine types	7
Surface Plasmon Resonance (SPR) biosensor for detection of mycotoxins: A review	7
Sandwich-type immunosensor based on COF-LZU1 as the substrate platform and graphene framework supported nanosilver as probe for CA125 detection	7
Cryopreservation of peripheral blood mononuclear cells for use in proliferation assays: First step towards potency assays	7
Brucella antigens (BhuA, 7α-HSDH, FliC) in poly I:C adjuvant as potential vaccine candidates against brucellosis	6

An optimized flow cytometry panel for classifying macrophage polarization	6
Development of novel highly sensitive methods to detect endogenous cGAMP in cells and tissue	6
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species	6
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses	6
An in vitro refolding method to produce oligomers of anti-CHIKV, E2-IgM Fc fusion subunit vaccine candidates expressed in E. coli	6
Culturing cells with mast cell phenotype and function: Comparison of peripheral blood and bone marrow as a source.	6
Detergent wash improves vaccinated lymph node handling ex vivo	6
Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination	6
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection	6
Clinical and analytical evaluation of the Abbott AdviseDx quantitative SARS-CoV-2 IgG assay and comparison with two other serological tests	6
Development of a time-resolved fluorescence immunoassay based on immunomagnetic beads for gastrin-17	6
Characterization of CAR T cell expansion and cytotoxic potential during Ex Vivo manufacturing using image-based cytometry	6
Novel immunoassay for diagnosis of ongoing Clostridioides difficile infections using serum and medium enriched for newly synthesized antibodies (MENSA)	6
Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of sows	6
Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay	6
An optimized flow cytometry protocol for simultaneous detection of T cell activation induced markers and intracellular cytokines: Application to SARS-CoV-2 immune individuals	6
Positive tissue transglutaminase antibodies with negative endomysial antibodies: Unresolved issues in diagnosing celiac disease	5
A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensor	5
A light-initiated chemiluminescent assay for the detection of children's milk protein-specific IgE with excellent ability to avoid interference of specific IgG	5
A standarized protocol for evaluation of large extracellular vesicles using the attune™ NXT system	5
Autotransporter-based surface expression and complementation of split TreA fragments utilized for the detection of antibodies against bovine leukemia virus	5
Production, characterization and in-vitro applications of single-domain antibody against thyroglobulin selected from novel T7 phage display library	5
An eight-plex immunoassay for Group A streptococcus serology and vaccine development	5
A sensitive method to quantify HIV-1 antibodies in mucosal samples	5
Easy pan-detection of human IgA immunoglobulins	5
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination	5
Novel bridge multi-species ELISA for detection of SARS-CoV-2 antibodies	5
Impact of complement and difference of cell-based assay and ELISA in determination of neutralization capacity against mumps and measles virus	5
Relationship between circulating tumor-associated autoantibodies and clinical outcomes in advanced-stage NSCLC patients receiving PD-1/−L1 directed immune checkpoint inhibition.	5
Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy	5
A new method for identification of outliers in immunogenicity assay cut point data	5
Development and qualification of a fast, high-throughput and robust imaging-based neutralization assay for respiratory syncytial virus	5
Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies	5
Feasibility of using monocyte-derived dendritic cells obtained from cryopreserved cells for DC-based vaccines	5
A method to study protein biomarkers in saliva using an automated capillary nano-immunoassay platform (Wes™)	5
Development and optimization of a Zika virus antibody-dependent cell-mediated cytotoxicity (ADCC) assay	5
Non-glycosylated SARS-CoV-2 RBD elicited a robust neutralizing antibody response in mice	5
The crux of C1-INH testing in everyday lab work	5
Hybrid recombinant Omp 22, 25, and 31 immunodominant epitopes can be used for serodiagnosis of brucellosis	5
Isolation and culture of rat intestinal mucosal microvascular endothelial cells using immunomagnetic beads	5
Human primary neutrophil mRNA does not contaminate human resolving macrophage mRNA after efferocytosis	5
Small p53 derived peptide suitable for robust nanobodies dimerization	5
Development of competitive inhibition ELISA as an effective potency test to analyze human rabies vaccines and assessment of the antigenic epitope of rabies glycoprotein	5
Evaluation of ELISA and VNT for sheeppox virus antibody detection and development of an immunoenzymatic quantitative method	4
Broad immunophenotyping of the murine brain tumor microenvironment	4
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen	4
The co-stimulation of anti-CD28 and IL-2 enhances the sensitivity of ELISPOT assays for detection of neoantigen-specific T cells in PBMC	4
Development and validation of a novel immunogenicity assay to detect anti-drug and anti-PEG antibodies simultaneously with high sensitivity	4
A multi-colour confocal microscopy method for identifying and enumerating macrophage subtypes and adherent cells in the stromal vascular fraction of human adipose	4
Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay	4
Evaluation of antibody titer kinetics and SARS-CoV-2 infections in a large cohort of healthcare professionals ten months after administration of the BNT162b2 vaccine	4
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma	4
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	4
In silico engineering a CD80 variant with increased affinity to CTLA-4 and decreased affinity to CD28 for optimized cancer immunotherapy	4
Production and characterization of novel monoclonal antibodies against outer membrane protein A (OmpA) of Acinetobacter baumannii	4
Gene knockout in highly purified mouse hematopoietic stem cells by CRISPR/Cas9 technology	4
Urinary cell mRNA profiling of kidney allograft recipients: A systematic investigation of a filtration based protocol for the simplification of urine processing	4
Blood biomarker algorithms for the diagnosis of mycoplasma pneumoniae respiratory infections	4
Acquired B-cell deficiency secondary to B-cell-depleting therapies	4
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	4
Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination	4
An innovative method for characterizing neutralizing antibodies against antibody-derived therapeutics	4
Murine monoclonal antibodies against RBD of the SARS-CoV-2 spike protein as useful analytical tools for subunit vaccine development and clinical trials	4
Performance of a commercially available multiplex platform in the assessment of circulating cytokines and chemokines in patients with rheumatoid arthritis and osteoarthritis	4
Allergen extraction: Factors influencing immunogenicity and sensitivity of immunoassays	4
A simple, robust flow cytometry-based whole blood assay for investigating sex differential interferon alpha production by plasmacytoid dendritic cells	4
A new cell line for assessing HIV-1 antibody dependent cellular cytotoxicity against a broad range of variants	4
Distinct and overlapping effects of β2-glycoprotein I conformational variants in ligand interactions and functional assays	4
Comparison of different immunoassays for the detection of antibodies against Intrinsic Factor and Parietal Cells	4
Adaption of a conventional ELISA to a 96-well ELISA-Array for measuring the antibody responses to influenza virus proteins and vaccines	4
A novel whole blood based method for lymphocyte transformation test in drug allergies	4
Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants	4