OOIR: Observatory of International Research

Papers

(The TQCC of Journal of Immunological Methods is 4. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-12-01 to 2025-12-01.)

Article	Citations
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp	63
Establishing biological variation of immunological and inflammatory biomarkers in healthy individuals: a longitudinal study	48
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges	28
Candidate antibody reference reagents for Chlamydia trachomatis serology	23
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis	23
Editorial Board	22
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia	19
Agreement and error of titration assays	19
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	18
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes	17
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring	17
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells	17
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials	16
Editorial Board	16
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells	16
Antibodies against chikungunya virus structural proteins reveal the presence of CHIKV virions in migrasomes	14
Advancing cervical cancer screening: A novel in-house ELISA assay for the simultaneous detection of p16 and Ki-67	13
Smartphone based lateral flow immunoassay quantifications	13
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution	13
Development of a new automated IL-6 immunoassay	13
Development and validation of dengue virus envelope protein domain III IgG antibody enzyme-linked immunosorbent assay	13
Divalent cations and SERPIN gene expression modulate horseshoe crab coagulation under LPS induction	12
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells	12
Development of an ELISA strategy for the serological diagnosis of farmer's lung disease	12
Editorial Board	12

Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent	12
An unexpected bioanalytical challenge caused by positive control antibodies in a clinical immunogenicity assay – A simple solution and broadly applicable recommendations	11
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays	11
Automated EliA dsDNA assay: correlation with Crithidia lucillea indirect immunofluorescent test	11
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing	11
Cell-based potency assay for anti-CD3-anti-CD19 diabody	11
Quantitative flow cytometry using quantitative streptavidin-protein G-biotin beads (qBeads)	11
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment	11
Leptospirosis detection: correspondence	10
A systematic examination of anti-drug antibody titer estimation: Applied recommendations	10
Preparation and specific identification of monoclonal antibodies targeting the VP2 structural protein of minute virus of canines	10
Editorial Board	10
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B	9
Target tolerance improvement of the immunogenicity assay developed for analysing samples from clinical trials of RGB-14, a proposed biosimilar to Prolia/Xgeva	9
Evaluation of self-collected dried blood spots for detection of SARS-CoV-2 nucleocapsid antibodies shows low sensitivity	9
A 33-color panel of phenotypic analysis of murine organ specific immune cells	9
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen	8
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay	8
Preliminary field evaluation of indirect ELISA test using the recombinant antigen rLicNTPDase-2 for serodiagnosis of canine leishmaniasis in Colombia	8
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study	8
Temperature stability of rabies neutralizing antibodies	8
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis	8
Optimization of blood sampling time for isolation of antigen binding single-domain antibodies from immunized llamas	8
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)	8
Development of sandwich enzyme-linked immunosorbent assays quantifying mouse urinary megalin, a novel proximal tubular biomarker	8
Inducing and regulating human naive CD4+ T cell proliferation by different antigen presenting cells	8
Indirect ELISAs with sucrose subcellular fractions of Neospora caninum as antigens for diagnosis of neosporosis in cattle	7
Magnetic CAR T cell purification using an anti-G4S linker antibody	7
High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design	7
Comparative evaluation of pretreatment techniques for enhancing specificity in solid-phase bead-based immunoassays	7
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens	7
Murine model of steroid-resistant neutrophilic bronchial asthma as an attempt to simulate human pathology	7
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR	7
NADPH oxidase subunit p22 : A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells	7
Analysis of the lot-to-lot evaluation process for enzyme-linked immunosorbent assays (ELISA)	7
Monoclonal antibody on-rate constant determined from time-course data of ligand binding by capture ELISA: Evaluation of eight data analysis methods	7
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity	7
Evaluation and correlation between SARS-CoV-2 neutralizing and binding antibodies in convalescent and vaccinated subjects	7
Persistence of criteria antiphospholipid antibodies in relation to titers, isotype and antiphospholipid syndrome diagnosis	7
Comparative evaluation of Luminex based assays for detection of SARS-CoV-2 antibodies in a transplantation laboratory	7
Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff	7
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay	7
A novel strategy to investigate the factors regulating the Treg to Tfr transition during acute viral infection	6
Editorial Board	6
Strategies to determine positive anti-SARS-CoV-2 memory T lymphocyte response during the evolution of an epidemic	6
Improved accessibility of the high precision T-SPOT®.TB test for laboratories and patients through the automation of cell isolation	6
True volumetric counting of CD34+ cells using flow cytometry	6
In silico designed Staphylococcus aureus B-cell multi-epitope vaccine did not elicit antibodies against target antigens suggesting multi-domain approach	6
Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies	6
Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes	6

Long term investigation of formulation buffers to mitigate stability issues of conjugated critical reagents	6
Nephelometry vs. Immunoturbidimetry assay: Analytical performance on IgG subclasses	6
Antisera against flagellin A or B inhibits Pseudomonas aeruginosa motility as measured by novel video microscopy assay	6
Development and evaluation of i-Tracker risankizumab and i-Tracker anti-risankizumab kits: fast and innovative chemiluminescent assays for the monitoring of patients treated with Risankizumab	6
Development and assessment of a new bioassay for accurate quantification of Type I interferons induced by bovine respiratory viruses	6
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals	6
Development and validation of an in vitro titrimetric method for determination of classical swine fever viruses in PK-15 cells	6
Isolation and expansion of murine γδ T cells from mouse splenocytes	6
A novel panel of peptides with diagnostic potential for serological identification of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii in human sera	6
An optimized flow cytometry panel for classifying macrophage polarization	6
Performance characteristics of a vascular endothelial growth factor-D (VEGF-D) ELISA for diagnostic and therapeutic monitoring	6
Production and characterization of novel monoclonal antibodies against outer membrane protein A (OmpA) of Acinetobacter baumannii	6
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen	6
Validation of dried blood spot sampling for detecting SARS-CoV-2 antibodies and total immunoglobulins in a large cohort of asymptomatic young adults	6
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies	6
Celiac disease risk stratification: correlation of HLA genotype and celiac-specific antibody positivity	5
A note of caution for using calmodulin antibodies	5
Development and optimization of a diluted whole blood ELISpot assay to test immune function	5
Study of avidity-ELISA: Comparison of chaotropic agents, incubation temperature and affinity maturation after meningococcal immunization	5
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade	5
Editorial Board	5
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination	5
Acute kidney allograft dysfunction is associated with increased number of non-HLA antibodies in absence of donor specific HLA antibodies	5
Editorial Board	5
1,25-dihydroxyvitamin D3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells	5
Development of a Bak gene based standard curve for absolute quantification of BK virus in real time quantitative PCR assay and noninvasive diagnosis of BK virus nephropathy in kidney allograft recipie	5
Overcoming cross-reactivity of antibodies against human lactate dehydrogenase	5
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation	5
An enzyme-free method for isolating testicular macrophages from rodent models	5
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays	5
Development of a proficiency testing program for HPV serology assays used to evaluate antibody responses in vaccine trials	5
Development and characterization of an ETV1 rabbit monoclonal antibody for the immunohistochemical detection of ETV1 expression in cancer tissue specimens	5
Impact of oral Bacillus Calmette–Guérin on B220 receptor expression in Peyer's patches of lactating mice: Analysis of receptor percentiles and cell frequencies	5
The Fas-mediated apoptosis assay: From concept to clinical application	5
Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses	5
Editorial Board	5
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis	5
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response	5
Flow cytometry for B-cell subset analysis in immunodeficiencies	4
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood	4
Stochastic interpretation of basophil activation test improves diagnostic reliability	4
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment	4
Long-term cell storage of RAW 264.7 cells in a deep freezer dampens M2 marker expression	4
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue	4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay	4
The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG1	4
A high-throughput assay to measure antibodies that block adhesion of Plasmodium falciparum infected erythrocytes to chondroitin sulfate A.	4
Complement function and activation in human serum and plasma collected in different blood collection tubes	4
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of	4
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice	4
Development of an enteric pathogen multiplex immunoassay to measure antibody responses in blood and saliva for integrated serology applications	4
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library	4
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA242	4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response	4
Highly effective strategy for isolation of mononuclear cells from frozen cord blood	4
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments	4
Assessment of TREC-based NBS SCID reporting practices for harmonization of results and interpretation: a global survey	4
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA	4
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood	4
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus	4
Dissociation of murine oral mucosal tissues for single cell applications	4
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens	4
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue	4
Editorial Board	4
Editorial Board	4
A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody	4
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera	4
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections	4