Journal of Immunological Methods

Papers
(The median citation count of Journal of Immunological Methods is 1. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-01-01 to 2025-01-01.)
ArticleCitations
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells63
Mapping antibody binding using multiplexed epitope substitution analysis50
Editorial Board42
Editorial Board39
Non-invasive image-based cytometry for high throughput NK cell cytolysis analysis34
A chimeric HLA-A2:β2M:Ig fusion protein for the study of virus-specific CD8+ T-cells31
Development of a mechanism of action reflective and robust potency assay for a therapeutic antibody against alpha toxin using rabbit erythrocytes26
Development of a rapid lateral flow immunochromatography assay for the detection of group-specific antibodies against VP7 protein of bluetongue virus in multiple species25
Development of ELISA for the detection of antibodies against VP2 protein of bluetongue virus serotype-122
Editorial Board18
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis18
A novel method for in vitro culture and expansion of nonhuman primate B cells17
Editorial Board16
Dried blood spots for the quantitative evaluation of IgG isotypes and correlation with serum samples in HIV-exposed uninfected (HEU) infants16
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring16
Construction of a full-length antibody phage display vector15
Immunoglobulin isotype compositions of ABO specific antibodies are dependent on the individual patient blood group and blood group specificity: Results from a healthy donor cohort14
Establishment of a homogeneous immunoassay-light-initiated chemiluminescence assay for detecting anti-Müllerian hormone in human serum14
Editorial Board13
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute13
Characterization and ex vivo expansion of rare in situ cytokine secreting T cell populations from tumor tissue and blood of oral squamous cell carcinoma patients13
Establishment of a novel cell-based assay using HLA-transfected cells to detect HLA antibodies13
Optimisation and validation of a new method for antibody dependent cellular phagocytosis in hepatitis C virus infection13
A sensitive and less cytotoxic assay for identification of proliferating T cells based on bioorthogonally-functionalized uridine analogue13
Role of antinuclear antibodies in COVID-19 patients13
Optimized cytotoxicity assay for co-suspended effector and target cells12
Comparability study of monocyte derived dendritic cells, primary monocytes, and THP1 cells for innate immune responses12
A single homogeneous assay for simultaneous measurement of bispecific antibody target binding12
Development of rELISA using novel markers for the diagnosis of paratuberculosis12
Thanking the reviewers of Journal of Immunological Methods11
Critical evaluation of fluorescent dyes to evaluate the stability and ligand binding properties of an anti-cocaine mAb, h2E211
Development of a new automated IL-6 immunoassay11
Immunoassay for detection of oligomeric proteins11
Editorial Board11
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer11
Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response10
Editorial Board10
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes10
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials10
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays10
Editorial introduction for “B cell immunodeficiencies” Journal of Immunological Methods special edition10
An enzyme-free method for isolating testicular macrophages from rodent models10
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia10
Editorial Board10
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens9
SARS-CoV-2 variants lacking a functional ORF8 may reduce accuracy of serological testing9
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing9
Digital RT-PCR Chip method for detection of SARS-CoV-2 virus9
Quantitative determination of C-polysaccharide in Streptococcus pneumoniae capsular polysaccharides by enzyme-linked immunosorbent assay9
Development of competitive inhibition ELISA as an effective potency test to analyze human rabies vaccines and assessment of the antigenic epitope of rabies glycoprotein9
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis9
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine8
Comparison of hemagglutination inhibition tests, immunoperoxidase monolayer assays, and serum neutralizing tests in detecting antibodies against blue eye disease in pigs8
Selection of positive controls and their impact on anti-drug antibody assay performance8
Robust and low-cost ELISA based on IgG-Fc tagged recombinant proteins to screen for anti-SARS-CoV-2 antibodies8
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue8
Development of gold nanocluster complex for the detection of tumor necrosis factor-alpha based on immunoassay8
Analytical performance of lateral flow immunoassay for SARS-CoV-2 exposure screening on venous and capillary blood samples8
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults8
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood8
Comparison of single-chain variable fragments and monoclonal antibody against dihydroartemisinin8
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution7
Development of immunochromatographic strip assay to detect recent infection of Japanese encephalitis virus in swine population7
A sensitive method to quantify HIV-1 antibodies in mucosal samples7
Detection of drug-specific immunoglobulin E (IgE) and acute mediator release for the diagnosis of immediate drug hypersensitivity reactions7
Development of a flow cytometry-based potency assay for prediction of cytokine storms induced by biosimilar monoclonal antibodies7
A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells7
Immunodetection of Furcraea Necrotic Streak Virus-FNSV in fique plants (Furcraea macrophylla Baker) using a polyclonal antibody IgY produced in chicken egg yolk7
Characterization of a rat monoclonal antibody raised against ferroptotic cells7
Development of a defibrinated human blood hemolysis assay for rapid testing of hemolytic activity compared to computational prediction7
Development of an indirect competitive enzyme linked immunosorbent assay for the quantitative detection of Mycoplasma hyopneumoniae during the vaccine production process6
Interactions of human mesenchymal stromal cells with peripheral blood mononuclear cells in a Mitogenic proliferation assay6
Candidate antibody reference reagents for Chlamydia trachomatis serology6
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells6
Development of an ELISA for an effective potency determination of recombinant rabies human monoclonal antibody6
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA2426
Parameters of immunoglobulin extraction from dried blood spot cards and immunoassays for detection of antibody response to pathogens including the novel SARS-CoV-26
Small p53 derived peptide suitable for robust nanobodies dimerization6
Editorial – Immunobiophysics: Advances and techniques6
Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay6
Development and characterization of an indirect ELISA to detect SARS-CoV-2 spike protein-specific antibodies6
Overview of human B-cell development and antibody deficiencies6
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials6
Smartphone based lateral flow immunoassay quantifications6
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp6
Agreement and error of titration assays6
Feasibility of using monocyte-derived dendritic cells obtained from cryopreserved cells for DC-based vaccines6
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors5
Dissociation of murine oral mucosal tissues for single cell applications5
Multiplex bead binding assays using off-the-shelf components and common flow cytometers5
Surface Plasmon Resonance (SPR) biosensor for detection of mycotoxins: A review5
Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of sows5
Isolation of phage-antibodies against Eimeria species that infect chickens5
Novel immunoassay for diagnosis of ongoing Clostridioides difficile infections using serum and medium enriched for newly synthesized antibodies (MENSA)5
Fine definition of the epitopes on the human gp91 /NOX2 for the monoclonal antibodies CL-5 and 485
Validation of dried blood spots samples for the screening of hepatitis B virus surface by enzyme immunoassay method5
Letter to the editor regarding “Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials”5
An easy assay to detect autoantibodies neutralizing cytokines in subjects with critical infections5
Editorial Board5
Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients5
Electrofusion preparation of anti-triazophos monoclonal antibodies for development of an indirect competitive enzyme-linked immunosorbent assay5
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue5
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides5
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus5
Development of a light-initiated chemiluminescence assay for the quantitative detection of cow's milk allergen-specific IgE5
Bacterial coloration immunofluorescence strip for ultrasensitive rapid detection of bacterial antibodies and targeted antibody-secreting hybridomas5
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids5
Epitope profiling of cow's milk allergen-specific antibodies with determining IgE content in epitopes-ALL, a 14-epitopes mixture5
Immunoinformatics and Pepscan strategies on the path of a peptide-based serological diagnosis of COVID195
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library5
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies4
Generation of good manufacturing practice grade virus-specific T cells for the management of post-transplant CMV infections4
A method for expansion and retroviral transduction of mouse regulatory T cells4
Comprehensive phenotyping of circulating immune cell subsets in people living with HIV4
Stability of anti-drug antibodies in human serum samples4
Editorial Board4
Evaluation of a multiplexed coronavirus antigen array for detection of SARS-CoV-2 specific IgG in COVID-19 convalescent plasma4
Evaluation of antibody titer kinetics and SARS-CoV-2 infections in a large cohort of healthcare professionals ten months after administration of the BNT162b2 vaccine4
Flow cytometry for B-cell subset analysis in immunodeficiencies4
Highly versatile antibody binding assay for the detection of SARS-CoV-2 infection and vaccination4
Monocyte monolayer assay in pre-transfusion testing: A magic key in transfusing patients with recurrent bad cross-match due to alloimmunization4
Reply to “Precision-cut human liver slice cultures as an immunological platform”4
In vitro differentiation of myeloid suppressor cells (MDSC-like) from an immature myelomonocytic precursor THP-14
Novel hapten design, highly sensitive monoclonal antibody production, and immunoassay development for rapid screening of illegally added chloramphenicol in cosmetics4
In vitro immunization approach to generate specific murine monoclonal IgG antibodies4
Rapid, sensitive and cost-effective determination of immune checkpoint inhibitor activity using a magnetic bead-based binding assay4
Ultrasensitive multiplexed chemiluminescent enzyme-linked immunosorbent assays in 384-well plates4
Editorial Board4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response4
Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep4
Potential diagnostic application of the baculovirus-expressed recombinant truncated nucleocapsid protein of peste des petits ruminants virus in ELISA4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay4
Assessing TCR identity, knock-in efficiency, and potency for individualized TCR-T cell therapy4
Editorial Board4
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing4
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice4
Neuronal binding by antibodies can be influenced by low pH stress during the isolation procedure3
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments3
Mitochondrial function in oral health and disease3
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood3
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood3
Retention of E-selectin functionalized liposome fanny packs on Jurkat cells following invasion through collagen3
An eight-plex immunoassay for Group A streptococcus serology and vaccine development3
Immunofluorescence analysis of human eosinophils3
Editorial Board3
Development and evaluation of a multi-antigen serological assay for the intra-vitam diagnosis of Tuberculosis caused by Mycobacterium bovis in pigs3
The C-terminus of Brucella abortus MviN induces humoral and cell mediated immune responses in BALB/c mice that protects against the virulent Brucella 544 challenge3
Effect of wash media type during PBMC isolation on downstream characterization of SARS-CoV-2-specific T cells3
Editorial – Special Issue on in vitro detection of drug allergy3
Individual polysaccharide quantification in polyvalent pneumococcal conjugate vaccine using rate nephelometry3
Detergent wash improves vaccinated lymph node handling ex vivo3
A novel whole blood assay to quantify the release of T cell associated cytokines in response to Bordetella pertussis antigens3
A novel antigen immunochromatography fluorometric strip for rapid detection and application of pathogenic bacterial high-quality antibody3
SARS-CoV-2 serology testing: Progress and challenges3
Development and evaluation of a low cost IgG ELISA test based in RBD protein for COVID-193
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?3
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a3
Flow cytometric determination of ferroptosis using a rat monoclonal antibody raised against ferroptotic cells3
Comparative assessment of cytometry by time-of-flight and full spectral flow cytometry based on a 33-color antibody panel3
Development of a non-biased, high-throughput ELISA for the rapid evaluation of immunogenicity and cross-reactivity3
Establishment of bioluminescent imaging model using murine T cell lymphoma susceptive to NK cell-dependent immune-surveillance3
Development and qualification of a fast, high-throughput and robust imaging-based neutralization assay for respiratory syncytial virus3
Highlights of the 33rd annual scientific meeting of the Association of Medical Laboratory Immunologists (AMLI)3
Detection of alloreactive T cells from cryopreserved human peripheral blood mononuclear cells3
High-yield production of recombinant human myelin oligodendrocyte glycoprotein in SHuffle bacteria without a refolding step3
Comparative analysis of LAG3 antibodies shows differential binding patterns by flow cytometry3
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing3
Generation of a cost-effective cell line for support of high-throughput isolation of primary human B cells and monoclonal neutralizing antibodies3
Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles3
Detection of RNA-dependent RNA polymerase of porcine epidemic diarrhea virus3
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols3
Evaluation of circulating leucocyte populations both in subjects with previous SARS-COV-2 infection and in healthy subjects after vaccination3
MILKSHAKE Western blot and Sundae ELISA: We all scream for better antibody validation3
ERAMER: A novel in silico tool for prediction of ERAP1 enzyme trimming3
Parameters of TNF receptor co-expression in allergic and autoimmune processes: Differences and diagnostic significance3
Editorial Board3
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies3
Clinical evaluation of commercial SARS-CoV-2 serological assays in a malaria endemic setting2
Comparison of three methods for the detection of antibodies against muscle-specific kinase2
Characterization of an IL-8 cleavage inhibition assay to determine the functionality of anti-SpyCEP antibodies in human sera2
A systematic examination of anti-drug antibody titer estimation: Applied recommendations2
Comparison of capillary blood self-collection using the Tasso-SST device with venous phlebotomy for anti-SARS-CoV-2 antibody measurement2
A method to isolate and cryopreserve cerebrospinal fluid mononuclear cells from external ventricular drains to investigate immunological processes in acute brain injuries2
Antinuclear antibodies (ANAs) detected by indirect immunofluorescence (IIF) method in acute COVID-19 infection; future roadmap for laboratory diagnosis2
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA2
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment2
The commutability of enzyme linked immunosorbent assays for the quantification of serum eosinophil-derived neurotoxin (EDN)2
Highly effective strategy for isolation of mononuclear cells from frozen cord blood2
Rhesus macaque Bcl-6/Bcl-xL B cell immortalization: Discovery of HIV-1 neutralizing antibodies from lymph node2
Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples2
Detection of engineered T cells in FFPE tissue by multiplex in situ hybridization and immunohistochemistry2
SARS-CoV-2 diagnostics: Towards a more comprehensive approach to routine patient testing2
Enrichment of circulating tumor-derived extracellular vesicles from human plasma2
Editorial Board2
Relationship between circulating tumor-associated autoantibodies and clinical outcomes in advanced-stage NSCLC patients receiving PD-1/−L1 directed immune checkpoint inhibition.2
Flow cytometry as a diagnostic tool in neuroblastoma2
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies2
New and rapid strategies for the diagnosis of bovine paratuberculosis “in situ” using latex particles2
Editorial Board2
The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG12
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay2
High-sensitive photometric microplate assay for tumor necrosis factor-alpha based on Fe@BC nanozyme2
Evaluation of a point of care lateral flow assay for antibody detection following SARS CoV-2 mRNA vaccine series2
Validation of the use of dried blood spots in a chikungunya virus IgG serological assay2
A homogeneous time-resolved fluorometric energy transfer assay for the binding assessment of FcRn with IgG antibodies2
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment2
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections2
“Common variable immunodeficiency: Challenges for diagnosis”2
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of 2
Editorial Board2
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments2
Optimized flow cytometry assays to monitor neutrophil activation in human and mouse whole blood samples2
Development of a sensitive and stable chemiluminescent immunoassay for detection of birch pollen allergic specific IgE based on recombinant Bet v1 protein2
Immunocartography: Charting vaccine-driven immunity by applying single cell proteomics to an in vitro human model2
Establishment of human post-vaccination SARS-CoV-2 standard reference sera2
Development and verification of an enzyme-linked immunosorbent assay for the quantification of toxoid A and toxoid B from Clostridioides difficile2
Immunodiagnostic usefulness of monoclonal antibodies specific to conformational epitopes of Taenia solium oncosphere protein TSOL182
Editorial Board2
Production, characterization and in-vitro applications of single-domain antibody against thyroglobulin selected from novel T7 phage display library2
Validation of a double-color ELISpot assay of IFN-γ and IL-4 production in human peripheral blood mononuclear cells2
Development and validation of a microfluidic multiplex immunoassay for the determination of levels and avidity of serum antibodies to tetanus, diphtheria and pertussis antigens2
Antibody engineering to generate anti-tumor-associated glycoprotein 72 mouse recombinant CC49 IgG with improved solubility, purity, and thermal stability2
Optimizing the method for expressing human monoclonal antibodies from a single peripheral blood cell from vaccinated donors2
Development of a long-term, IL7 dependent cell death rescue assay in CD4+ T-cells2
ELISpot assay as a diagnostic tool in drug hypersensitivity reactions2
Evaluation and optimization of the immunohistochemistry antigen retrieval methods on mouse decalcified joint tissues2
Identification of tumor-associated antigens of lung cancer: SEREX combined with bioinformatics analysis2
Editorial Board2
Novel IgE crosslinking-induced luciferase expression method using human-rat chimeric IgE receptor-carrying mast cells2
Quantification of the pro-form of human complement component factor D (adipsin)2
Leptospirosis detection: correspondence2
Editorial Board2
Development of an ELISA displaying similar reactivity with reduced and oxidized human Thioredoxin-1 (Trx1): The plasma level of Trx1 in early onset psychosis disorders2
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays2
Multiplex immunoassay to measure antibody response to nine HPV vaccine types2
Development of a novel capillary electrophoresis-based approach for detection of anti-drug antibody responses2
Identification and characterization of phage display-selected peptides having affinity to Peste des petits ruminants virus2
Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy2
Depletion of CD45RA+ T cells: Advantages and disadvantages of different purification methods2
Evaluation of commercially available antibodies and fluorescent conotoxins for the detection of surface ganglionic acetylcholine receptor on the neuroblastoma cell line, IMR-32 by flow cytometry2
A multi-colour confocal microscopy method for identifying and enumerating macrophage subtypes and adherent cells in the stromal vascular fraction of human adipose2
Nanobody-based microfluidic human Fc assay for preclinical plasma quantification of IgG1/1.1 and IgG1-Fc-conjugates2
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen2
Anti-cytokine autoantibodies and inborn errors of immunity1
Impact of complement and difference of cell-based assay and ELISA in determination of neutralization capacity against mumps and measles virus1
Editorial Board1
Murine model of steroid-resistant neutrophilic bronchial asthma as an attempt to simulate human pathology1
Concordance with established tests and reproducibility of results obtained with a new single antigen chip array for HLA antibody detection (HISTO SPOT® HLA AB)1
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity1
Editorial Board1
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