Journal of Immunological Methods

Papers
(The median citation count of Journal of Immunological Methods is 1. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2022-05-01 to 2026-05-01.)
ArticleCitations
Decreased variability in the site-specific results during participation in the External Quality Assurance Program Oversight Laboratory (EQAPOL) proficiency program for IFN-gamma enzyme-linked immunosp67
Establishing biological variation of immunological and inflammatory biomarkers in healthy individuals: a longitudinal study66
Antibodies against chikungunya virus structural proteins reveal the presence of CHIKV virions in migrasomes31
Appropriate immunoassay systems are needed to evaluate various anticentromere antibodies as biomarkers for disease progression in systemic sclerosis27
Editorial Board25
Inter-intra instrument comparison and standardization of a 10-color immunophenotyping for B and T cell non-Hodgkin lymphoma diagnosis and monitoring21
Phage display selection of human single domain antibodies towards karilysin, a metalloproteinase and secreted virulence factor from Tannerella forsythia21
Identifying immunoglobulin abnormalities in the presence of variable polyclonal background20
Editorial Board16
Development of optimized cytotoxicity assays for assessing the antitumor potential of CAR-T cells15
Candidate antibody reference reagents for Chlamydia trachomatis serology15
Development of a new automated IL-6 immunoassay14
Smartphone based lateral flow immunoassay quantifications14
A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials14
Combined RNAscope and immunohistochemistry staining on duodenal paraffin sections as a new tool to reveal cytolytic potential of intraepithelial lymphocytes14
Techniques for evaluating the ATP-gated ion channel P2X7 receptor function in macrophages and microglial cells14
Advancing cervical cancer screening: A novel in-house ELISA assay for the simultaneous detection of p16 and Ki-6713
A sensitive and drug tolerant assay for detecting anti-AAV9 antibodies using affinity capture elution13
Phospho-flow cytometry assays for diagnostic use – A discussion of assay utility and assay development and validation challenges12
Development and validation of dengue virus envelope protein domain III IgG antibody enzyme-linked immunosorbent assay12
Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation12
Editorial Board11
Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells11
Divalent cations and SERPIN gene expression modulate horseshoe crab coagulation under LPS induction11
Automated EliA dsDNA assay: correlation with Crithidia lucillea indirect immunofluorescent test11
Flow cytometry reveals constant lymphocyte proportions after long-term cryopreservation of whole blood in TransFix® cell stabilization reagent11
Quantitative flow cytometry using quantitative streptavidin-protein G-biotin beads (qBeads)10
Application of a novel branched-DNA assay to quantify killer immunoglobulin-like receptor (KIR) mRNA expression identifies tissue compartmentalization in naïve and SIV-infected rhesus macaques10
An unexpected bioanalytical challenge caused by positive control antibodies in a clinical immunogenicity assay – A simple solution and broadly applicable recommendations10
Development of anti-IgA monoclonal and polyclonal reagents for detecting mucosal and systemic IgA in nonhuman primates10
Dried blood spots are a valid alternative to venipuncture for COVID-19 antibody testing10
Development of an ELISA strategy for the serological diagnosis of farmer's lung disease10
Cell-based potency assay for anti-CD3-anti-CD19 diabody10
Editorial Board9
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: Comment9
Humoral and cellular response of two different vaccines against SARS-CoV-2 in a group of healthcare workers: An observational study9
A systematic examination of anti-drug antibody titer estimation: Applied recommendations9
Performance characteristics of the BRAHMS KRYPTOR automated squamous cell carcinoma antigen assay9
A 33-color panel of phenotypic analysis of murine organ specific immune cells8
Target tolerance improvement of the immunogenicity assay developed for analysing samples from clinical trials of RGB-14, a proposed biosimilar to Prolia/Xgeva8
Development of automated microfluidic immunoassays for the detection of SARS-CoV-2 antibodies and antigen8
Preparation and specific identification of monoclonal antibodies targeting the VP2 structural protein of minute virus of canines8
Comparison of real-time quantitative PCR and two digital PCR platforms to detect copy number variation in FCGR3B8
Current technologies for anti-ENA antibody detection: State-of-the-art of diagnostic immunoassays8
Evaluation of self-collected dried blood spots for detection of SARS-CoV-2 nucleocapsid antibodies shows low sensitivity8
Temperature stability of rabies neutralizing antibodies8
Cross-reactivity of T cell-specific antibodies in the bank vole (Myodes glareolus)8
Optimisation of SARS-CoV-2 peptide stimulation and measurement of cytokine output by intracellular flow cytometry and bio-plex analysis7
Comparative evaluation of pretreatment techniques for enhancing specificity in solid-phase bead-based immunoassays7
Inducing and regulating human naive CD4+ T cell proliferation by different antigen presenting cells7
Fragments of bombinakinin M exist in lipopolysaccharide-stimulated skin secretions of Bombina maxima and show lipopolysaccharide-neutralizing activity7
A novel panel of peptides with diagnostic potential for serological identification of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii in human sera7
An optimized flow cytometry panel for classifying macrophage polarization7
Murine model of steroid-resistant neutrophilic bronchial asthma as an attempt to simulate human pathology7
Magnetic CAR T cell purification using an anti-G4S linker antibody7
A cell-based subtractive panning strategy for selection of conformation-specific single-chain variable-fragment (scFv) against dimerization domain of EGFR7
Indirect ELISAs with sucrose subcellular fractions of Neospora caninum as antigens for diagnosis of neosporosis in cattle7
Analysis of the lot-to-lot evaluation process for enzyme-linked immunosorbent assays (ELISA)7
NADPH oxidase subunit p22 : A marker of oxidase-dependent oxidative stress and target for stress suppression in nonphagocytic cells7
Preliminary field evaluation of indirect ELISA test using the recombinant antigen rLicNTPDase-2 for serodiagnosis of canine leishmaniasis in Colombia7
Editorial Board7
Development of sandwich enzyme-linked immunosorbent assays quantifying mouse urinary megalin, a novel proximal tubular biomarker7
Optimization of a versatile cell ELISA for detection of antibodies against membrane protein BCMA7
Monoclonal antibody on-rate constant determined from time-course data of ligand binding by capture ELISA: Evaluation of eight data analysis methods7
Optimization of blood sampling time for isolation of antigen binding single-domain antibodies from immunized llamas7
Persistence of criteria antiphospholipid antibodies in relation to titers, isotype and antiphospholipid syndrome diagnosis7
Identifying therapeutic monoclonal antibodies using target protein collision electrophoresis reflex assay to separate the wheat from the chaff7
Comparative evaluation of Luminex based assays for detection of SARS-CoV-2 antibodies in a transplantation laboratory7
Long term investigation of formulation buffers to mitigate stability issues of conjugated critical reagents7
A comprehensive method for the phenotypical and functional characterization of recalled human memory B and T cells specific to vaccine antigens7
Determination of echinococcosis IgG antibodies using magnetic bead-based chemiluminescence immunoassay7
Development and validation of an in vitro titrimetric method for determination of classical swine fever viruses in PK-15 cells6
Strategies to determine positive anti-SARS-CoV-2 memory T lymphocyte response during the evolution of an epidemic6
Isolation and expansion of murine γδ T cells from mouse splenocytes6
Nephelometry vs. Immunoturbidimetry assay: Analytical performance on IgG subclasses6
Standardization, validation, and comparative evaluation of a faster and high-performance test for quantification of yellow fever neutralizing antibodies6
Effect of storage time on peripheral blood mononuclear cell isolation from blood collected in vacutainer CPT™ tubes6
Development and assessment of a new bioassay for accurate quantification of Type I interferons induced by bovine respiratory viruses6
Development and evaluation of i-Tracker risankizumab and i-Tracker anti-risankizumab kits: fast and innovative chemiluminescent assays for the monitoring of patients treated with Risankizumab6
Impact of oral Bacillus Calmette–Guérin on B220 receptor expression in Peyer's patches of lactating mice: Analysis of receptor percentiles and cell frequencies6
Validation of dried blood spot sampling for detecting SARS-CoV-2 antibodies and total immunoglobulins in a large cohort of asymptomatic young adults6
In silico designed Staphylococcus aureus B-cell multi-epitope vaccine did not elicit antibodies against target antigens suggesting multi-domain approach6
Evaluation of refractometry methods for estimating passive immunity status in neonatal foals6
True volumetric counting of CD34+ cells using flow cytometry6
Performance characteristics of a vascular endothelial growth factor-D (VEGF-D) ELISA for diagnostic and therapeutic monitoring6
Improved accessibility of the high precision T-SPOT®.TB test for laboratories and patients through the automation of cell isolation6
1,25-dihydroxyvitamin D3 augments low-dose PMA-based monocyte-to-macrophage differentiation in THP-1 cells6
Development of a high-throughput image cytometric screening method as a research tool for immunophenotypic characterization of patient samples from clinical studies6
A novel strategy to investigate the factors regulating the Treg to Tfr transition during acute viral infection6
Antisera against flagellin A or B inhibits Pseudomonas aeruginosa motility as measured by novel video microscopy assay6
Development of a proficiency testing program for HPV serology assays used to evaluate antibody responses in vaccine trials5
Development and optimization of a diluted whole blood ELISpot assay to test immune function5
Methodological approaches to optimize multiplex oral fluid SARS-CoV-2 IgG assay performance and correlation with serologic and neutralizing antibody responses5
ELISA method to assign weight-based antibody concentration to antigen-specific Ig in sera5
Publisher's note: Challenges for complement functional assays in the clinical laboratory: From test validation to clinical interpretation5
Study of avidity-ELISA: Comparison of chaotropic agents, incubation temperature and affinity maturation after meningococcal immunization5
Celiac disease risk stratification: correlation of HLA genotype and celiac-specific antibody positivity5
Editorial Board5
Performance and validation of an adaptable multiplex assay for detection of serologic response to SARS-CoV-2 infection or vaccination5
The Fas-mediated apoptosis assay: From concept to clinical application5
Editorial Board5
Establishment of growth stimulating gene 2 protein time-resolved fluorescence immunoassay and its application in sepsis5
Development of a Bak gene based standard curve for absolute quantification of BK virus in real time quantitative PCR assay and noninvasive diagnosis of BK virus nephropathy in kidney allograft recipie5
A note of caution for using calmodulin antibodies5
VaxArray immunoassay for the multiplexed quantification of poliovirus D-antigen5
Overcoming cross-reactivity of antibodies against human lactate dehydrogenase5
Miniaturized CAR knocked onto CD3ε extends TCR function with CAR specificity under control of endogenous TCR signaling cascade5
Editorial Board5
Development and characterization of an ETV1 rabbit monoclonal antibody for the immunohistochemical detection of ETV1 expression in cancer tissue specimens5
Highly effective strategy for isolation of mononuclear cells from frozen cord blood4
The mouse monoclonal antibody 4E3 is specific for the G1m17 allotype of human IgG14
Acute kidney allograft dysfunction is associated with increased number of non-HLA antibodies in absence of donor specific HLA antibodies4
A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments4
Isolation of anti-Ancylostoma-secreted protein 5 (ASP5) antibody from a naïve antibody phage library4
Complement function and activation in human serum and plasma collected in different blood collection tubes4
Editorial Board4
30-color full spectrum flow cytometry panel for deep immunophenotyping of T cell subsets in murine tumor tissue4
Evaluation of cell-mediated immunity of E.coli nanovaccines in chickens4
Plaque reduction neutralization test for smallpox vaccines: Laboratory optimization and validation method for immunogenicity assessment4
Development of an enteric pathogen multiplex immunoassay to measure antibody responses in blood and saliva for integrated serology applications4
Cell-type-specific gene expression profile by laser capture microdissection on mirror sections4
Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA4
A flow cytometry-based assay to determine the ability of anti-Streptococcus pyogenes antibodies to mediate monocytic phagocytosis in human sera4
Assessment of TREC-based NBS SCID reporting practices for harmonization of results and interpretation: a global survey4
Tumor nano-lysate activates dendritic cells to evoke a preventative immune response4
Artificial intelligence and deep learning to map immune cell types in inflamed human tissue4
Dissociation of murine oral mucosal tissues for single cell applications4
Rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of varicella-zoster virus immunoglobulin G antibodies in fingerstick blood4
Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays4
Clinical utility of the lymphocyte proliferation assay, an in vitro functional readout of the adaptive immune response4
Long-term cell storage of RAW 264.7 cells in a deep freezer dampens M2 marker expression4
Diagnosis of septic shock by serum measurement of human neutrophil lipocalin by a rapid homogeneous assay4
Editorial Board4
A peptide derived from the N-terminal of NS2A for the preparation of ZIKV NS2A recognition polyclonal antibody4
A high-throughput assay to measure antibodies that block adhesion of Plasmodium falciparum infected erythrocytes to chondroitin sulfate A.4
A universal urinary cell gene signature of acute rejection in kidney allografts4
Comparison of two anti-histones antibodies commercial assays in an immunology laboratory and systemic lupus erythematosus4
Letter to the editor concerning “Development and validation of indirect ELISA for antibody detection against different protein antigens of porcine epidemic diarrhea virus in the colostrum and milk of 4
Stochastic interpretation of basophil activation test improves diagnostic reliability4
Optimized flow cytometry protocol for dihydrorhodamine 123-based detection of reactive oxygen species in leukocyte subpopulations in whole blood4
Novel mRNA vaccines targeting epitope-rich regions of avian coronavirus enhance immunogenic efficacy4
Sensitive amplified luminescent proximity homogeneous assay for the quantitative detection of CA2424
An enzyme-free method for isolating testicular macrophages from rodent models4
Generation of an alpaca serum that induces immune-mediated crescentic glomerulonephritis in mice4
Flow cytometry for B-cell subset analysis in immunodeficiencies4
Differences in mouse strains determine the outcome of Der p 2 allergy induction protocols4
Editorial Board4
A comprehensive multiparameter flow cytometry panel for immune profiling and functional studies of frozen tissue, bone marrow, and spleen3
Development, validation and stability study of an ELISA method to quantify the major allergen Jun a 1 in Juniperus ashei extracts3
PD-BAT: A novel approach of pooling basophil donors for expansion of commercial laboratory testing of Chronic Spontaneous Urticaria3
A taxonomy of multiple regression methods for immunologists3
ELISA protein detector (EPD): A Python-based ELISA tool for accurate low-level protein quantification3
In silico engineering a CD80 variant with increased affinity to CTLA-4 and decreased affinity to CD28 for optimized cancer immunotherapy3
Description of a non-competitive ELISA based on time course analysis of ligand binding at saturation, and a direct method for calculating the affinity of monoclonal antibodies3
Preparation and characterization of a neutralizing murine monoclonal antibody against tetanus toxin3
Editorial Board3
Platelets isolation and ectonucleotidase assay: Revealing functional aspects of the communication between the vasculature and the immune system3
Identification of monoclonal antibodies from naive antibody phage-display libraries for protein detection in formalin-fixed paraffin-embedded tissues3
Novel bridge multi-species ELISA for detection of SARS-CoV-2 antibodies3
Mayaro virus and chikungunya virus IgM and IgG testing in parallel improves diagnosis of acute Mayaro virus infections3
Application of microphysiologic system to assess neutrophil extracellular trap in xenotransplantation3
Reference intervals for functional lymphocyte proliferation studies using 3H thymidine uptake in adults3
Multiplex ACE2-RBD binding inhibition assay: An integrated tool for assessing neutralizing antibodies to SARS-CoV-2 variants and protection against breakthrough infections3
Chemiluminescent detection of platelet derived growth factor-BB based on sandwich label-free aptasensor and biotin–streptavidin strategy3
Basophil activation test; User's manual3
Development of a novel anti-PEG antibody assay enabling investigation of potential immunogenicity triggered by the PEG moiety of biotherapeutics3
Editorial Board3
Highly sensitive detection of carcinoembryonic antigen via an electrochemical platform fabricated by CCLP- gold nanocomposite3
Editorial Board3
Two technologies, one goal: Developing and evaluating anti-Zika virus NS1 antibodies using phage display and hybridoma technologies for diagnostic applications3
Expression of bluetongue virus full-length VP7 protein in insect cells and its diagnostic utility for detection of antibodies to the virus infection3
Data to determination: transitioning to new methodology for better patient care, a process improvement journey of a clinical immunology laboratory in a pediatric hospital3
Chimeric protein A/G conjugate based lateral flow assay for the rapid detection of brucellosis in multiple animal species3
Immunoinformatics-guided designing of epitope-based subunit vaccine from Pilus assembly protein of Acinetobacter baumannii bacteria3
COVID-19 in patients with B cell immune deficiency3
Subclass optimised antibodies as an effective tool to suppress protein A induced false positives in immunoassays detecting staphylococcal enterotoxin B3
Editorial Board3
Evaluation of multiple immunoassay formats for detection of anti-drug antibodies to zinpentraxin alfa3
Editorial Board3
A dried blood spot test for diagnosis of autoimmune pulmonary alveolar proteinosis3
Theoretical and practical considerations for validating antigen-specific B cell ImmunoSpot assays3
Optimized flow cytometry panel for the detection and analysis of human tumor-induced memory-like NK cells3
Detection of autoantibodies in bullous pemphigoid, epidermolysis bullosa acquisita, and pemphigus vulgaris by indirect immunofluorescence with a BIOCHIP Mosaic3
Rapid identification of tumor-reactive T-cell receptors by RNA preamplification-based single-cell sequencing2
Editorial Board2
Comparison of methods to isolate peripheral blood mononuclear cells from cattle blood2
Application of the SARS-CoV-2-S1 ACE-2 receptor interaction as the basis of the fully automated assay to detect neutralizing SARS-CoV-2-S1 antibodies in blood samples2
Development and performance evaluation of a novel, fully automated, high-throughput GAD65 autoantibody assay on the Phadia platform2
Evaluation of a point of care lateral flow assay for antibody detection following SARS CoV-2 mRNA vaccine series2
Human monoclonal antibody cloning and expression with overlap extension PCR and short DNA fragments2
Performance evaluation of an ELISA for measuring risankizumab and antibodies to risankizumab as compared to quantitation by mass spectrometry and electrochemiluminescence2
Editorial Board2
Methyl ester and aromatic ether modification of mitragynine for generation of mitragynine-specific polyclonal antibodies2
Detection of soy proteins in processed foods using a novel enzyme-linked immunosorbent assay with a polyclonal antibody against Gly m 52
Discrepancies between two total IgE assays and difference in reference intervals in healthy adults2
Novel IgE crosslinking-induced luciferase expression method using human-rat chimeric IgE receptor-carrying mast cells2
A novel HRM-based gap-qRT-PCR for identification and quantitation of the vaccine and field strain(s) of lumpy skin disease virus2
A flexible gradient lateral flow immunochromatographic assay for qualitative, semi-quantitative, and quantitative determination of serum amyloid A2
A novel immuno-molecular strategy for the detection of Streptococcus pneumoniae serotypes in human cerebrospinal and middle ear fluids2
Immunogenicity of tislelizumab: clinical summary of ADA incidence and feasibility of assessing the suitability of validation cut points using statistical methods2
Differential composition and yield of leukocytes isolated from various blood component leukoreduction filters2
High throughput screening and assessment of a bioinformatics pipeline for novel marker discovery in relapsing/refractory multiple myeloma2
Optimization of lateral flow assay for Canine morbillivirus detection and the application of the strip as sample substitute2
Antibody detection by agglutination-PCR (ADAP) assays for the analysis of tissue transglutaminase autoantibodies in celiac disease2
Sources of variability in Luminex bead-based cytokine assays: Evidence from twelve years of multi-site proficiency testing2
Antibody engineering to generate anti-tumor-associated glycoprotein 72 mouse recombinant CC49 IgG with improved solubility, purity, and thermal stability2
Shifting beyond the gel: comparison of therapeutic antibody gel shift assays to mass spectrometry for evaluating multiple myeloma patient serum samples2
Assessment of mimicking by EBV-CMV immunoglobulin M of anti-HLA antibodies2
Optimization of IgG1 immune complexes to stimulate cytokine production in innate cells2
Advanced hybridoma technology for selective production of high-affinity monoclonal antibodies through B-cell receptors2
Comparison of two chagas ELISA methods: why are the signal to cutoff ratios important?2
Dependence of the amplification factor on the body water deuterium enrichment2
Isolation and culture of rat intestinal mucosal microvascular endothelial cells using immunomagnetic beads2
Generation of Q-bead against bone Gla protein with simplified preparation steps2
Fit-for-purpose validation of a drug-tolerant immunogenicity assay for a human mAb drug in animal safety studies2
SARS-CoV-2 spike protein expression as an identification in quality control testing for Adenovector based COVID-19 vaccine2
Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies2
Development and laboratory validation of an electrochemiluminescence ELISA technique for measuring infliximab concentrations and anti-drug antibodies2
Stabilization of leukocytes from cerebrospinal fluid for central immunophenotypic evaluation in multicenter clinical trials2
Validation of a live cell based assay for the detection of MOG antibodies by flow cytometry2
A rapid and high-throughput T cell immunophenotyping assay for cellular therapy bioprocess using the Cellaca® PLX image cytometer2
Editorial Board2
Identification of the linear Fc-binding epitope on the bovine IgG1 Fc receptor of (boFcγRI) using synthetic peptides2
Peptide Hamming Graphs: A network representation of peptides presented through specific HLAs to identify potential epitope clusters2
Fresh versus frozen PMBC using the SARS CoV-2 T-SPOT.COVID test. Which works best?2
Comparison of mixed modeling regression methods for the assessment of longitudinal CyTOF® data2
Repeatability of QuantiFERON-TB gold plus testing utilizing microparticle chemiluminescence2
Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols2
Optimizing the method for expressing human monoclonal antibodies from a single peripheral blood cell from vaccinated donors2
Skeletal muscle macrophage ablation in mice2
Indirect ELISA diagnostic based on recombinant 3AB3 nonstructural protein of foot-and-mouth disease virus for use in porcine serology2
Impact of bridge heterology on functional parameters of ELISA for 17α-methyltestosterone2
A high-throughput assay using dysfunctional T cells for phenotypic screening immune checkpoint modulators2
The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system a2
Repurposing discarded leukodepletion filters as a source of mononuclear cells for advanced in vitro research1
Dried serum spots on pre-punched filter paper discs are ready-to-use storage and shipping devices for blood-borne antigens and antibodies1
Development and validity assessment of ELISA test with recombinant chimeric protein of SARS-CoV-21
Yclon: Ultrafast clustering of B cell clones from high-throughput immunoglobulin repertoire sequencing data1
Parallel application of two ELISA systems: anti-BP230 and anti-BP180-NC16A-4X provide the most sensitive serological diagnosis of bullous pemphigoid1
Editorial Board1
Development of a multiplexing potency assay using upconverting nanoparticles-based luminescence resonance energy transfer1
Editorial Board1
Peripheral blood and nasal swabs Type I and III IFNs signature in RSV positive infant with bronchiolitis1
Immunoglobulin treatment for B-cell immunodeficiencies1
Belimumab concentration measurements using a homologous anti-idiotype immunoassay1
Enhancing the accuracy of serological diagnosis for muscle-specific tyrosine kinase antibodies through dual laboratory technique integration1
Development of a high-sensitivity assay for direct quantitation of EGFr on extracellular vesicles in plasma1
Accurate recognition and characterization of biclonal gammopathies by mass spectrometry1
Detection and consistency of mucosal fluid T lymphocyte phenotypes and their relationship with blood, age and gender1
From phlebotomy to peripheral blood mononuclear cell isolation; effect of time and early-life HIV/ART exposure on cell yield and viability in paediatric samples in a high HIV prevalence setting1
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