(The TQCC of Cryobiology is 11. The table below lists those papers that are above that threshold based on CrossRef citation counts. The publications cover those that have been published in the past four years, i.e., from 2019-06-01 to 2023-06-01.)
Cryoprotectants: A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures272
Mesenchymal stromal cells derived from various tissues: Biological, clinical and cryopreservation aspects236
Freezing injury from “solution effects” and its prevention by natural or artificial cryoprotection189
The Grand Challenges of Organ Banking: Proceedings from the first global summit on complex tissue cryopreservation95
The cryoprotective effect of antifreeze glycopeptides from antarctic fishes95
Factors Affecting Leakage of Trapped Solutes from Phospholipid Vesicles during Thermotropic Phase Transitions93
An extracellular ice-binding glycoprotein from an Arctic psychrophilic yeast87
Cryopreservation of adult human hepatocytes obtained from resected liver biopsies76
Cryopreservation affects ROS-induced oxidative stress and antioxidant response in Arabidopsis seedlings75
Thermal requirements in vitro of epidermal cells from seals73
Characterization of an antifreeze protein from the polar diatom Fragilariopsis cylindrus and its relevance in sea ice71
Antifreeze Glycoproteins from Antarctic Notothenioid Fishes Fail to Protect the Rat Cardiac Explant during Hypothermic and Freezing Preservation60
The effects of cryopreservation on cells isolated from adipose, bone marrow and dental pulp tissues60
Anhydrobiotic Engineering of Bacterial and Mammalian Cells: Is Intracellular Trehalose Sufficient?58
Freezing behavior of aqueous solutions of glycoproteins from the blood of an Antarctic fish58
Control of sperm concentration is necessary for standardization of sperm cryopreservation in aquatic species: Evidence from sperm agglutination in oysters57
Recovery and reproduction of an Antarctic tardigrade retrieved from a moss sample frozen for over 30 years57
A technique for the separation and cryopreservation of myeloid stem cells from human bone marrow56
Osmotic Effects on Feline Spermatozoa from Normospermic versus Teratospermic Donors55
Cryopreservation of spermatozoa from wild-born Namibian cheetahs (Acinonyx jubatus) and influence of glycerol on cryosurvival54
Supercooling and ice propagation in blood from arctic fishes51
Status of cryopreservation of embryos from domestic animals51
Some factors affecting loss of intracellular enzymes from spermatozoa50
Ice-active proteins from the Antarctic nematode Panagrolaimus davidi50
Characterization of partially purified glutathione reductase from cold-hardened and nonhardened spinach leaf tissue50
Psychrotolerant bacteria isolated from the leaf apoplast of cold-adapted wild plants improve the cold resistance of bean (Phaseolus vulgaris L.) under low temperature49
Relation of several antioxidant enzymes to rapid freezing resistance in suspension cultured cells from alpine Chorispora bungeana49
Shedding off specific lipid constituents from sperm cell membrane during cryopreservation49
Evaluation of intracellular and extracellular trehalose as a cryoprotectant of stem cells obtained from umbilical cord blood48
Cryopreservation of sperm from the marine shrimp Sicyonia ingentis47
Boar sperm cryosurvival is better after exposure to seminal plasma from selected fractions than to those from entire ejaculate45
Characterization of the ice-binding protein from Arctic yeast Leucosporidium sp. AY3044
Distribution and Characterization of Recrystallization Inhibitor Activity in Plant and Lichen Species from the UK and Maritime Antarctic43
Viable Spermatozoa Can Be Recovered from Refrigerated Mice up to 7 Days after Death41
Taxonomic characterization, adaptation strategies and biotechnological potential of cryophilic yeasts from ice cores of Midre Lovénbreen glacier, Svalbard, Arctic41
Myocardial mechanical dysfunction and calcium overload following rewarming from experimental hypothermia in vivo41
Machine perfusion at 20°C reduces preservation damage to livers from non-heart beating donors41
Functional testing of hepatocytes following their recovery from cryopreservation39
Freeze tolerance and accumulation of cryoprotectants in the enchytraeid Enchytraeus albidus (Oligochaeta) from Greenland and Europe38
Effects of low-molecular-weight fractions (LMWF) from milk, egg yolk, and seminal plasma on freezability of bovine spermatozoa38
Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: influence of adding and removing cryoprotectant37
Expression and characterization of an antifreeze protein from the perennial rye grass, Lolium perenne37
Intracellular ice formation in yeast cells vs. cooling rate: Predictions from modeling vs. experimental observations by differential scanning calorimetry37
Ice-binding proteins from enoki and shiitake mushrooms36
Cryopreservation-induced alterations in protein tyrosine phosphorylation of spermatozoa from different portions of the boar ejaculate36
Kinetics of water loss from cells at subzero centigrade temperatures36
Effects of cryopreservation methods on post-thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii36
Cryopreservation of shoot tips of recalcitrant and tropical species: Advances and strategies36
Improved survival after cryogenic exposure of shoot tips derived from in vitro plantlet cultures of potato35
Extrusion and removal of lipid from the cytoplasm of porcine oocytes at the germinal vesicle stage: Centrifugation under hypertonic conditions influences vitrification35
Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri35
Effect of cryopreservation on expression of Th1 and Th2 cytokines in blood mononuclear cells from patients with different cytokine profiles, analysed with three common assays: an overall decrease of i34
Melatonin protects rabbit spermatozoa from cryo-damage via decreasing oxidative stress34
Blastocysts derived from in vitro-fertilized cat oocytes after vitrification and dilution with sucrose34
Effects of low temperature on contraction in papillary muscles from rabbit, rat, and hedgehog34
Function and quality of kidneys after cold storage, machine perfusion, or retrograde oxygen persufflation: Results from a porcine autotransplantation model33
Cells from an anhydrobiotic chironomid survive almost complete desiccation33
Comparative cryopreservation study of trochophore larvae from two species of bivalves: Pacific oyster (Crassostrea gigas) and Blue mussel (Mytilus galloprovincialis)32
Natural cryoprotectants combinations of l-proline and trehalose for red blood cells cryopreservation32
Trehalose transporter from African chironomid larvae improves desiccation tolerance of Chinese hamster ovary cells32
Rapid and uniform electromagnetic heating of aqueous cryoprotectant solutions from cryogenic temperatures32
Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species31
Isolation of Ice-Nucleating Active Bacteria from the Freeze-Tolerant Frog, Rana sylvatica31
Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic30
Antioxidative effect of melatonin on cryopreserved chicken semen30
Osmotic tolerance of mouse spermatozoa from various genetic backgrounds: Acrosome integrity, membrane integrity, and maintenance of motility30
Cryopreservation of Spermatozoa from Freeze-Tolerant and -Intolerant Anurans30
Cryopreservation of sperm from seven-band grouper, Epinephelus septemfasciatus30
Freeze tolerance in Aporrectodea caliginosa and other earthworms from Finland30
Vitrification of goat, sheep, and cattle skin samples from whole ear extirpated after death and maintained at different storage times and temperatures29
Cryopreservation of human embryonic stem cells by a programmed freezer with an oscillating magnetic field29
Membrane Reorganization during Chilling: Implications for Long-Term Stabilization of Platelets29
Characterization of a family of ice-active proteins from the Ryegrass, Lolium perenne28
Determination of Human Platelet Membrane Permeability Coefficients Using the Kedem–Katchalsky Formalism: Estimates from Two- vs Three-Parameter Fits28
Air-dried cells from the anhydrobiotic insect, Polypedilum vanderplanki, can survive long term preservation at room temperature and retain proliferation potential after rehydration28
Thermal stability properties of an antifreeze protein from the desert beetle Microdera punctipennis28
Factors in the cryopreservation of bone marrow cells from children with acute lymphocytic leukemia27
Analytical prediction of the temperature field emanating from a cryogenic surgical cannula26
Effective surface-based cryopreservation of human embryonic stem cells by vitrification26
Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout26
Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application25
Characterization of a novel β-helix antifreeze protein from the desert beetle Anatolica polita24
Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose24
Cryoprotectant delivery and removal from murine insulinomas at vitrification-relevant concentrations24
Purification and characterisation of an antifreeze protein from Forsythia suspensa (L.)24
Preservation of human normal and leukemic cells with dimethyl sulfoxide at −80°C24
Preservation of female genetic resources of common carp through oogonial stem cell manipulation23
A technical bias: Differences in cooling rates prevent ampoules from being a reliable index of stem cell cryopreservation in large volumes23
Heterologous expression, refolding and functional characterization of two antifreeze proteins from Fragilariopsis cylindrus (Bacillariophyceae)22
Leakage of a trapped fluorescent marker from liposomes: Effects of eutectic crystallization of NaCl and internal freezing22
Direct ethanol fermentation from lignocellulosic biomass by Antarctic basidiomycetous yeast Mrakia blollopis under a low temperature condition22
Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation22
Serum-free cryopreservation of human amniotic epithelial cells before and after isolation from their natural scaffold22
Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature21
The preservation of erythrocytes at liquid nitrogen temperatures with hydroxyethyl starch: the removal of hydroxyethyl starch from erythrocytes after thawing21
Porcine embryo production following in vitro fertilization and intracytoplasmic sperm injection from vitrified immature oocytes matured with a granulosa cell co-culture system21
Characteristics of stem cells from human exfoliated deciduous teeth (SHED) from intact cryopreserved deciduous teeth21
Extracellular calcium protects cultured rat hepatocytes from injury caused by hypothermic preservation21
An investigation of the effects from a urethral warming system on temperature distributions during cryoablation treatment of the prostate: A phantom study20
Hypothermia protects H9c2 cardiomyocytes from H2O2 induced apoptosis20
Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species20
Ice-Nucleating Bacteria from the Guts of Two Sub-Antarctic Beetles,Hydromedion sparsutumandPerimylops antarcticus(Perimylopidae)20
Multipotent stromal cells derived from common marmoset Callithrix jacchus within alginate 3D environment: Effect of cryopreservation procedures19
Optimization of a protective medium for enhancing the viability of freeze-dried Bacillus amyloliquefaciens B1408 based on response surface methodology19
Cryopreservation of lipid bilayers by LEA proteins from Artemia franciscana and trehalose19
Osmotic parameters of cells from a bioengineered human corneal equivalent and consequences for cryopreservation19
Characterization and modulation of human mesenchymal stem cell stress pathway response following hypothermic storage19
Fertilization of C57BL/6 mouse sperm collected from cauda epididymides after preservation or transportation at 4°C using laser-microdissected oocytes19
Cryo-scanning electron microscopy (Cryo-SEM) of semen frozen in medium-straws from good and sub-standard freezer AI-boars19
Effects of mint, thyme, and curcumin extract nanoformulations on the sperm quality, apoptosis, chromatin decondensation, enzyme activity, and oxidative status of cryopreserved goat semen19
Anti-ice nucleation activity in xylem extracts from trees that contain deep supercooling xylem parenchyma cells19
Insights on cryoprotectant toxicity from gene expression profiling of endothelial cells exposed to ethylene glycol18
Cryopreservation of mantle dissociated cells from Haliotis tuberculata (Gastropoda) and postthawed primary cell cultures18
Melatonin rescues the aneuploidy in mice vitrified oocytes by regulating mitochondrial heat product18
Does fennel extract ameliorate oxidative stress frozen-thawed ram sperm?18
Comparative analysis of milk fat decomposition activity by Mrakia spp. isolated from Skarvsnes ice-free area, East Antarctica18
Establishment and characterization of a fibroblast line from Simmental cattle18
Effects of supplementation of Tris-egg yolk extender with royal jelly on chilled and frozen-thawed ram semen characteristics18
Effectiveness of human mesenchymal stem cells derived from bone marrow cryopreserved for 23–25years18
Purification and Characterization of Protein Kinase A from Liver of the Freeze-Tolerant Wood Frog: Role in Glycogenolysis during Freezing18
Cryopreservation of embryogenic tissues from mature holm oak trees18
Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents18
Binder of Sperm Proteins protect ram spermatozoa from freeze-thaw damage17
Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity17
Cardiovascular effects of levosimendan during rewarming from hypothermia in rat17
Changes of pollen viability of ornamental plants after long-term preservation in a cryopreservation pollen bank17
Investigations of motility and fertilization potential in thawed cryopreserved mouse sperm from cold-stored epididymides17
Induction of summer hibernation in the 13-lined ground squirrel shown by comparative serum transfusions from Arctic mammals17
An assessment of methods used for measuring the recovery of mammalian cells from freezing and thawing17
Effects of different concentration and combinations of cryoprotectants on sperm quality, functional integrity in three Indian horse breeds17
Influence of freezing with liquid nitrogen on whole-knee joint grafts and protection of cartilage from cryoinjury in rabbits17
Influence of cryoprotective agent and cooling rate on frozen and thawed hemocytes from the Mollusk Haliotis tuberculata17
Generation of Dendritic Cells from Fresh and Frozen Cord Blood CD34+Cells17
Expression and localization of an ice nucleating protein from a soil bacterium, Pseudomonas borealis16
Conventional freezing vs. cryoprotectant-free vitrification of epididymal (MESA) and testicular (TESE) spermatozoa: Three live births16
Purification and partial characterization of an ice nucleator protein from the intertidal gastropod, Melampus bidentatus16
The pH-dependent recovery of smooth muscle from storage at −13 °C in unfrozen media16
Natural deep eutectic systems, an emerging class of cryoprotectant agents16
Spermatozoa from the maned wolf (Chrysocyon brachyurus) display typical canid hyper-sensitivity to osmotic and freezing-induced injury, but respond favorably to dimethyl sulfoxide16
Me2SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugars16
The anti-oxidant roles of Taurine and Hypotaurine on acrosome integrity, HBA and HSPA2 of the human sperm during vitrification and post warming in two different temperature16
Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients16
Different cryopreservation requirements in foetal versus adult skin cells from an endangered mammal, the Iberian lynx (Lynx pardinus)16
Effects of cryopreservation at various temperatures on the survival of kelp grouper ( Epinephelus moara ) embryos from fertilization with cryopreserved sperm15
Different susceptibility of liver grafts from lean and obese Zucker rats to preservation injury15
Algae Permeability to Me2SO from −3 to 23°C15
Effects of exercise in a cold environment on gene expression for mitochondrial biogenesis and mitophagy15
Maintenance of stem cell viability and differentiation potential following cryopreservation within 3-dimensional hyaluronic acid hydrogels15
Mild hypothermia protects hippocampal neurons from oxygen-glucose deprivation injury through inhibiting caspase-3 activation15
Optimization of cryopreservation condition for hematopoietic stem cells from umbilical cord blood15
Vitrification of Two-Cell Rat Embryos Derived from Immature HypothyroidrdwRats byin VitroFertilization in Ethylene Glycol-Based Solutions15
Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders15
Comparison among different cryoprotectants for cryopreservation of epididymal sperm from agouti (Dasyprocta leporina)15
A Pilot Study of Cryochemotherapy for Hepatic Metastases from Colorectal Cancer15
Water sorption isotherms from freeze-dried muscle fibers15
Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender15
Cryobanking the genetic diversity in the critically endangered Iberian lynx (Lynx pardinus) from skin biopsies. Investigating the cryopreservation and culture ability of highly valuable explants and c14
The ideal holding time for boar semen is 24 h at 17 °C prior to short-cryopreservation protocols14
Comparison of the effect of temperature on kidney cortex mitochondria from rabbit, dog, pig, and human: Arrhenius plots of ADP-stimulated respiration14
Workshop report: Cryopreservation of aquatic biomedical models14
The stability during low-temperature storage of an antifreeze protein isolated from the roots of cold-acclimated carrots14
Potent humanin analogue (HNG) protects human sperm from freeze-thaw-induced damage14
Improvement of direct ethanol fermentation from woody biomasses by the Antarctic basidiomycetous yeast, Mrakia blollopis, under a low temperature condition13
Effects of method of preservation on functions of livers from fed and fasted rabbits13
Cryopreservation of murine testicular Leydig cells by modified solid surface vitrification with supplementation of antioxidants13
Purification, crystal structure determination and functional characterization of type III antifreeze proteins from the European eelpout Zoarces viviparus13
Functional characteristics and responses to adrenergic stimulation of isolated heart preparations from hypothermic and hibernating subjects13
Cryopreservation of mammalian embryos: Derivation of a method13
Cryopreservation of sperm from the coral Acropora humilis13
Osmotic tolerance limits of red blood cells from umbilical cord blood13
Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques13
Characterization of two copper/zinc superoxide dismutases (Cu/Zn-SODs) from the desert beetle Microdera punctipennis and their activities in protecting E. coli cells against cold13
Change of supercooling capability in solutions containing different kinds of ice nucleators by flavonol glycosides from deep supercooling xylem parenchyma cells in trees12
A novel function – Thermal protective properties of an antifreeze protein from the summer desert beetle Microdera punctipennis12
Vitreous preservation of articular cartilage from cryoinjury in rabbits12
Metabolic reorganization in winter: Regulation of pyruvate dehydrogenase (PDH) during long-term freezing and anoxia12
Evaluation of five additives to mitigate toxicity of cryoprotective agents on porcine chondrocytes12
Strong tolerance to freezing is a major survival strategy in insects inhabiting central Yakutia (Sakha Republic, Russia), the coldest region on earth12
Effect of activin A and insulin-like growth factor-I on in vitro development of preantral follicles isolated from cryopreserved ovarian tissues in the mouse12
Protection from nonfreezing cold injury by quinacrine, a phospholipase inhibitor12
Two-step accelerating freezing protocol yields a better motility, membranes and DNA integrities of thawed ram sperm than three-steps freezing protocols12
Micro-quantity straw as a carrier for cryopreservation of oligozoospermic semen samples: Effects of storage times and cryoprotectant12
Myocardial protection by propolis during prolonged hypothermic preservation12
Effects of temperature, potassium concentration, and sugar on human spermatozoa motility: A cell preservation model from reproductive medicine12
Effects of cryopreservation techniques on the preservation of ear skin – An alternative approach to conservation of jaguar, Panthera onca (Linnaeus, 1758)12
Effect of resveratrol on vitrified in vitro produced bovine embryos: Recovering the initial quality12
Toxicity tests of cryoprotecting agents for Mytilus galloprovincialis (Lamark, 1819) early developmental stages12
Survival of tissue balls from the coral Pocillopora damicornis L. exposed to cryoprotectant solutions11
Challenge from the simple: Some caveats in linearization of the Boyle–van’t Hoff and Arrhenius plots11
Supplementary cryoprotective effect of carboxylated ε-poly-l-lysine during vitrification of rat pancreatic islets11
Microfluidic method reduces osmotic stress injury to oocytes during cryoprotectant addition and removal processes in porcine oocytes11
Factors affecting the removal of low-molecular-weight fractions (LMWF) from egg yolk and seminal plasma in extended semen by dialysis: Effect on post-thaw sperm survival11
Combination of intracellular cryoprotectants preserves the structure and the cells proliferative capacity potential of adult collared peccary testicular tissue subjected to solid surface vitrification11
Effect of different concentrations of l-carnitine in extender for semen cryopreservation in sheep11
Foundations of modeling in cryobiology—II: Heat and mass transport in bulk and at cell membrane and ice-liquid interfaces11
Differential scanning calorimeter analyses of membrane lipids isolated from hardened and unhardened black locust bark and from winter rye seedlings11
Summer hibernation induced in ground squirrels (Citellus tridecemlineatus) by urine or plasma from hibernating bats (Myotis lucifugus or Eptesicus fuscus)11
Use of trehalose in the semen cryopreservation of Amazonian catfish Leiarius marmoratus11
Thermal expansion of substrate may affect adhesion of Chinese hamster fibroblasts to surfaces during freezing11
Bone morphogenetic protein activity preservation with extracorporeal irradiation- and liquid nitrogen freezing-treated recycled autografts for biological reconstruction in malignant bone tumor11
Effect of polyvinyl alcohol on survival and function of angora buck spermatozoa following cryopreservation11
The effect of a hydrogen sulfide releasing molecule (Na2S) on the cold storage of livers from cardiac dead donor rats. A study in an ex vivo model11