Biotechnology and Bioengineering

Papers
(The TQCC of Biotechnology and Bioengineering is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-05-01 to 2025-05-01.)
ArticleCitations
Concentration of clarified pool by single‐pass tangential flow filtration to improve productivity of protein A capture step: Impact of clarification strategies152
106
Super7 passaging method to improve Chinese hamster ovary cell fed‐batch performance101
63
Optical discrimination of pathological red blood cells62
An engineered ultrahigh affinity bi‐paratopic uPAR targeting agent confers enhanced tumor targeting52
Concentration‐dependent diffusion of unlabeled protein within an in vitro hyaluronic acid matrix48
Iron in blood cells: Function, relation to disease, and potential for magnetic separation47
Effects of biofilm heterogeneity on the apparent mechanical properties obtained by shear rheometry46
Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin46
Application and progress of 3D tumor models in breast cancer46
Back Cover Image, Volume 118, Number 11, November 202144
Cover Image, Volume 118, Number 8, August 202143
Outside Back Cover Image, Volume 118, Number 7, July 202141
Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost‐derived consortium41
Constructing multi‐enzymatic cascade reactions for selective production of 6‐bromoindirubin from tryptophan in Escherichia coli40
Continuous low pH viral inactivation: Operation and scaling strategy informs viral clearance study39
Double‐reporter‐guided targeted activation of the oxytetracycline silent gene cluster in Streptomyces rimosus M52739
Harnessing metabolic plasticity in CHO cells for enhanced perfusion cultivation38
Dual‐targeting multifunctional hyaluronic acid ligand‐capped gold nanorods with enhanced endo‐lysosomal escape ability for synergetic photodynamic–photothermal therapy of cancer38
Expression of filaments of theGeobacterextracellular cytochrome OmcS inShewanella oneidensis37
High‐throughput screening of optimal process conditions using model predictive control36
Current Advances and Future Prospects of Bulk and Microfluidic‐Enabled Electroporation Systems36
Biotechnology and Bioengineering: Volume 122, Number 5, May 202536
Simple, rapidly electroassembled thiolated PEG‐based sensor interfaces enable rapid interrogation of antibody titer and glycosylation34
pH Threshold Impacts Chalcopyrite Bioleaching Dynamics for the Extreme Thermoacidophile Sulfurisphaera ohwakuensis33
Evaluating potential of tissue‐engineered cryogels and chondrocyte derived exosomes in articular cartilage repair33
Dual‐Stage Cross‐Flow Filtration: Integrated Capture and Purification of Virus‐Like Particles33
Heterologous Expression of Candida antarctica Lipase B in Aspergillus niger Using CRISPR/Cas9‐mediated Multi‐Gene Editing32
Knowledge transfer across cell lines using hybrid Gaussian process models with entity embedding vectors31
Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green alga Chlorella sorokiniana for biomass production31
Computational fluid dynamics based digital twins of fixed bed bioreactors validate scaling principles for recombinant adeno‐associated virus gene therapy manufacturing31
Application of gas diffusion electrodes in bioeconomy: An update31
Technical‐scale biophotovoltaics for long‐term photo‐current generation from Synechocystis sp. PCC680330
Enhanced bioprocess control to advance the manufacture of mesenchymal stromal cell‐derived extracellular vesicles in stirred‐tank bioreactors30
Engineering an Overflow‐Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production29
Continuous polyhydroxybutyrate production from biogas in an innovative two‐stage bioreactor configuration29
Repressing expression of difficult‐to‐express recombinant proteins during the selection process increases productivity of CHO stable pools29
IR780 loaded gelatin‐PEG coated gold core silica shell nanorods for cancer‐targeted photothermal/photodynamic therapy29
Culture system for longitudinal monitoring of bone dynamics ex vivo29
Cover Image, Volume 118, Number 9, September 202129
Increased Mevalonate Production Using Engineered Citrate Synthase and Phosphofructokinase Variants of Escherichia coli29
Improved production of heme using metabolically engineered Escherichia coli28
Analyzing protein conjugation reactions for antibody‐drug conjugate synthesis using polarized excitation emission matrix spectroscopy28
Characterization and designing of an SAM riboswitch to establish a high‐throughput screening platform for SAM overproduction in Saccharomyces cerevisiae27
Biocatalytic role of cytochrome P450s to produce antibiotics: A review27
A robust multinutrient kinetic model for enhanced lutein and biomass yields in mixotrophic microalgae cultivation: A step towards successful large‐scale productions26
Accelerating attribute‐focused process and product development through the development and deployment of autonomous process analytical technology platform system26
Semiautomated design and soluble expression of a chimeric antigen TbpAB01 from Glaesserella parasuis26
Physiological Response of Penicillium chrysogenum to Mimicked Local and Global Perturbations of Substrate and Dissolved Oxygen Gradients at Industrial‐Scale26
Isothermal titration calorimetric assessment of lignin conversion by laccases25
A New Modeling Approach for Predicting the Growth of Filamentous Algae in Outdoor Algae‐Based Wastewater Treatment Systems25
Development of generic metabolic Raman calibration models using solution titration in aqueous phase and data augmentation for in‐line cell culture analysis24
Bioprinted dermis with human adipose tissue‐derived microvascular fragments promotes wound healing24
Experimental evolution reveals an effective avenue for d‐lactic acid production from glucose‐xylose mixtures via enhanced Glk activity and a cAMP‐independent CRP mutation24
Acoustic levitation as a tool for cell‐driven self‐organization of human cell spheroids during long‐term 3D culture24
Precise and reliable control of gene expression in Agrobacterium tumefaciens24
Culture‐free biphasic approach for sensitive detection of Escherichia coli O157:H7 from beef samples23
Reinforcement learning based temperature control of a fermentation bioreactor for ethanol production23
From spores to fungal pellets: A new high‐throughput image analysis highlights the structural development of Aspergillus niger23
Comparative genomic and transcriptomic analysis guides to further enhance the biosynthesis of erythromycin by an overproducer23
3D bioprinting and microscale organization of vascularized tissue constructs using collagen‐based bioink23
Kinetic characterization and modeling of sequentially entrapped enzymes in 3D‐printed PMMA microfluidic reactors for the synthesis of amorphadiene via the isopentenol utilization pathway22
Reviewing the process intensification landscape through the introduction of a novel, multitiered classification for downstream processing22
Machine learning approaches to root cause analysis, characterization, and monitoring of subvisible particles in monoclonal antibody formulations22
Erratum for “Constitutively active Rheb mutants [T23M] and [E40K] drive increased production and secretion of recombinant protein in Chinese hamster ovary cells” (Vol. 118, Issue 7, pp. 2422–2434)22
Enabling biocatalysis in high‐concentration organic cosolvent by enzyme gate engineering22
Microbial production of odd‐chain fatty acids22
Fine and combinatorial regulation of key metabolic pathway for enhanced β‐alanine biosynthesis with non‐inducible Escherichia coli22
Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli22
Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J922
Single Cell Protein Production From Ethanol: Model‐Based Bioreactor Operation at Industrial Scale21
Effect of protein fouling on filtrate flux and virus breakthrough behaviors during virus filtration process21
Metabolic analysis of the asparagine and glutamine dynamics in an industrial Chinese hamster ovary fed‐batch process21
Manufacturing a chimpanzee adenovirus‐vectored SARS‐CoV‐2 vaccine to meet global needs21
Streamlined Clarification and Capture Process for Monoclonal Antibodies Using Fluidized Bed Centrifugation and Multi‐Column Chromatography With Membrane Adsorbers21
Optimization of physical microenvironment to maintain the quiescence of human induced pluripotent stem cell‐derived hepatic stellate cells21
Bringing a scalable adaptive hybrid modeling framework closer to industrial use: Application on a multiscale fungal fermentation21
Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via “flow‐through affinity chromatography” using peptide‐based adsorbents21
Multi‐cell‐line learning for the data‐driven construction of mechanistic metabolic models21
Commitment of human mesenchymal stromal cells towards ACL fibroblast differentiation upon rAAV‐mediated FGF‐2 and TGF‐β overexpression using pNaSS‐grafted PCL films21
Biotechnological production of polyphosphate from industrial wash water21
Structural variability, implementational irregularities in mathematical modelling of polyhydroxyalkanoates (PHAs) production—A state‐of‐the‐art review21
Biotechnology and Bioengineering: Volume 121, Number 2, February 202421
Sulfur‐driven autotrophic denitrification of nitric oxide for efficient nitrous oxide recovery21
Increasing yield of in vitro transcription reaction with at‐line high pressure liquid chromatography monitoring21
Marine biological macromolecules as matrix material for biosensor fabrication21
Oxygen Consumption in Filamentous Pellets of Aspergillus niger: Microelectrode Measurements and Modeling21
Influence of circadian rhythm on drug metabolism in 3D hepatic spheroids21
Fabrication and in vitro evaluation of 3D printed porous silicate substituted calcium phosphate scaffolds for bone tissue engineering20
Artificial induction of chromosome aneuploidy in CHO cells alters their function as host cells20
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Recent Research on Chondrocyte Dedifferentiation and Insights for Regenerative Medicine20
Metal cofactor regulation combined with rational genetic engineering of Schizochytrium sp. for high‐yield production of squalene20
On‐column capping of poly dT media‐tethered mRNA accomplishes high capping efficiency, enhanced mRNA recovery, and improved stability against RNase20
Enhancing real‐time cell culture monitoring: Automated Raman model optimization with Taguchi method20
PCR‐ and wash‐free detection of serum miRNA via signaling probe hybridization20
Elucidating the effects of microbubble pinch‐off dynamics on mammalian cell viability20
Lactic acid bacteria as a tool for biovanillin production: A review20
Rapid spectrophotometric detection for optimized production of landomycins and characterization of their therapeutic potential20
Open‐Source Anaerobic Digestion Modeling Platform, Anaerobic Digestion Model No. 1 Fast (ADM1F)20
Dynamic optimization of an integrated cultivation‐aggregation model for mAb production20
A novel, scalable, and modular bioreactor design for dynamic simulation of the digestive tract19
Biotechnology and Bioengineering: Volume 118, Number 6, June 202119
Substrate and nutrient manipulation during continuous cultivation of extremophilic algae, Galdieria spp. RTK 37.1, substantially impacts biomass productivity and composition19
Directed evolution of unspecific peroxygenase in organic solvents19
Semirational engineering of an aldo–keto reductase KmAKR for overcoming trade‐offs between catalytic activity and thermostability19
Covalent organic frameworks (COFs) as carrier for improved drug delivery and biosensing applications19
Process monitoring framework for cross‐flow diafiltration‐based virus‐like particle disassembly: Tracing product properties and filtration performance19
Efficient biosynthesis of α‐aminoadipic acid via lysine catabolism in Escherichia coli19
Development of a novel purification method for AAV vectors using tangential flow filtration19
Pareto optimal metabolic engineering for the growth‐coupled overproduction of sustainable chemicals19
Resistive Method for Bacterial Detection Employing a Silicon Nanowire Electrical Chip18
Cover Image, Volume 121, Number 8, August 202418
Efficient and precise control of gene expression in Geobacter sulfurreducens through new genetic elements and tools for pollutant conversion18
A Paradigm of Computer Vision and Deep Learning Empowers the Strain Screening and Bioprocess Detection18
Platform development for high‐throughput optimization of perfusion processes: Part I: Implementation of cell bleeds in microwell plates18
Enhanced harvest performance predictability through advanced multivariate data analysis of mammalian cell culture particle size distribution18
Real‐Time Auto Controlling of Viable Cell Density in Perfusion Cultivation Aided by In‐Line Dielectric Spectroscopy With Segmented Adaptive PLS Model18
Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension‐Culture Cells of Cyclocarya paliurus18
H2O2 feeding enables LPMO‐assisted cellulose saccharification during simultaneous fermentative production of lactic acid18
Continuous monitoring of IgG using immobilized fluorescent reporters18
Automated calibration and in‐line measurement of product quality during therapeutic monoclonal antibody purification using Raman spectroscopy17
A proteomics approach to decipher a sticky CHO situation17
Effects of Air Temperature on Transient Expression of Influenza Hemagglutinin in Nicotiana benthamiana: Analysis of Transgene Transcription and Plant Stress Responses17
Engineered yeast for efficient de novo synthesis of 7‐dehydrocholesterol17
Cole‐Cole modeling of real‐time capacitance data for estimation of cell physiological properties in recombinant Escherichia coli cultivation17
Monitoring and control of the release of soluble O2 from H2O2 inside porous enzyme carrier for O2 supply to an immobilized d‐amino acid oxidase17
One‐Pot Hetero‐Di‐C‐Glycosylation of the Natural Polyphenol Phloretin by a Single C‐Glycosyltransferase With Broad Sugar Substrate Specificity17
Construction of a structural enzyme adsorption/kinetics model to elucidate additives associated lignin–cellulase interactions in complex bioconversion system17
Model‐based control system design to manage process parameters in mammalian cell culture for biopharmaceutical manufacturing17
Comparison of metabolic kinetics during high and low solids anaerobic digestion of fecal sludge17
Corrigendum to “High‐Throughput Investigation of Endothelial‐to‐Mesenchymal Transformation (EndMT) With Combinatorial Cellular Microarrays”17
Validation and scalability of homemade polycaprolactone macrobeads grafted with thermo‐responsive poly(N‐isopropylacrylamide) for mesenchymal stem cell expansion and harvesting17
Perfusion Process Intensification for Lentivirus Production Using a Novel Scale‐Down Model16
Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation16
Deep hybrid modeling of a HEK293 process: Combining long short‐term memory networks with first principles equations16
Reconstruction and analysis of genome‐scale metabolic model for thermophilic fungus Myceliophthora thermophila16
Fungal assisted bio‐treatment of environmental pollutants with comprehensive emphasis on noxious heavy metals: Recent updates16
Engineering Corynebacterium glutamicum for synthesis of poly(3‐hydroxybutyrate) from lignocellulose biomass16
A computational model for single cell Lamin‐A structural organization after microfluidic compression16
Design an Energy‐Conserving Pathway for Efficient Biosynthesis of 1,5‐Pentanediol and 5‐Amino‐1‐Pentanol16
Biotechnology and Bioengineering: Volume 120, Number 5, May 202316
Effects of pyruvate on primary metabolism and product quality for a high‐density perfusion process16
Engineering Escherichia coli asymmetry distribution‐based synthetic consortium for shikimate production16
Scale‐up economics for cultured meat16
Engineering an Escherichia coli based in vivo mRNA manufacturing platform16
Biotechnology and Bioengineering: Volume 121, Number 11, November 202416
Mesenchymal Stem Cells‐Derived Small Extracellular Vesicles and Their Validation as a Promising Treatment for Chondrosarcoma in a 3D Model in Vitro16
Engineering dense tumor constructs via cellular contraction of extracellular matrix hydrogels16
Optimized Biosynthetic Pathway for Nonnatural Amino Acids: An Efficient Approach for L‐2‐Aminobutyric Acid Production16
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Biotechnology and Bioengineering: Volume 120, Number 10, October 202316
On‐chip magnetophoretic capture in a model of malaria‐infected red blood cells15
Higher efficiency of vanadate iron in heterogeneous Fenton‐like systems to pretreat sugarcane bagasse and its enzymatic saccharification15
Role of carbon nanotubes (CNTs) in transgenic plant development15
Using MVDA with stoichiometric balances to optimize amino acid concentrations in chemically defined CHO cell culture medium for improved culture performance15
Identification of yttrium oxide‐specific peptides for future recycling of rare earth elements from electronic scrap15
Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stress15
Biosynthesis of allantoin in Escherichia coli via screening a highly effective urate oxidase15
Genome‐scale metabolic modeling of Thraustochytrium sp. RT2316‐16: Effects of nutrients on metabolism15
Cover Image, Volume 121, Number 2, February 202415
Cover Image, Volume 118, Number 7, July 202115
Structure‐function analysis of two closely related cutinases from Thermobifida cellulosilytica15
The usage of composite nanomaterials in biomedical engineering applications15
Sensitive analysis of single nucleotide variation by Cas13d orthologs, EsCas13d and RspCas13d15
Manipulation of mRNA translation elongation influences the fragmentation of a biotherapeutic Fc‐fusion protein produced in CHO cells15
Advanced cell technologies: Making protein, cell, and gene therapies a reality15
Enzyme‐functionalized alginate microparticles enable anaerobic culture under ambient oxygen15
Modeling large‐scale bioreactors with diffusion equations. Part II: Characterizing substrate, oxygen, temperature, carbon dioxide, and pH profiles15
Systems engineering Escherichia coli for efficient production p‐coumaric acid from glucose15
Effect of nutrient metabolism on cartilaginous tissue formation15
In silico and in vivo studies of the effect of surface curvature on the osteoconduction of porous scaffolds15
Scalable manufacturing platform for the production of PEGylated heme albumin14
Reducing phenotypic instabilities of a microbial population during continuous cultivation based on cell switching dynamics14
A multiscale modeling method for therapeutic antibodies in ion exchange chromatography14
An engineered cellobiohydrolase I for sustainable degradation of lignocellulosic biomass14
Plant extract‐based synthesis of metallic nanomaterials, their applications, and safety concerns14
Construction of a CRISPR‐Cas9‐Based Genetic Editing Tool for Serratia marcescens Using a Stationary Phase Promoter and Its Application in Putrescine Production14
Back Cover Image, Volume 120, Number 4, April 202314
Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturing14
Are K‐strategists yield‐strategists in disguise? An example from autotrophic nitrogen removal14
Titer improvement of milbemycins via coordinating metabolic competition and transcriptional co‐activation controlled by Streptomyces antibiotic regulatory protein family regulator in Strepto14
Binding stability of peptides to Co–Cr–Mo alloy affects proliferation and differentiation of osteoblast14
An adaptive soft‐sensor for advanced real‐time monitoring of an antibody‐drug conjugation reaction14
Sensitivity analysis and adaptive mutation strategy differential evolution algorithm for optimizing enzymes' turnover numbers in metabolic models13
Understanding the effects of aerodynamic and hydrodynamic shear forces on Pseudomonas aeruginosa biofilm growth13
Meso‐ and thermophilic posttreatment of press water coming from a thermophilic municipal solid waste digester13
Reducing Structural Nonidentifiabilities in Upstream Bioprocess Models Using Profile‐Likelihood13
Quorum sensing unveils the sludge floccule‐assisted stabilization of aerobic granules in granule‐dominated sequencing batch reactors13
N2‐fixation can sustain wastewater treatment performance of photogranules under nitrogen‐limiting conditions13
Transfer learning Bayesian optimization for competitor DNA molecule design for use in diagnostic assays13
Adjustment of the main biosynthesis modules to enhance the production of l‐homoserine in Escherichia coli W311013
Cover Image, Volume 119, Number 5, May 202213
Biotechnology and Bioengineering: Volume 118, Number 8, August 202113
Fibrin gel enhanced trilayer structure in cell‐cultured constructs13
The interplay of protein engineering and glycoengineering to fine‐tune antibody glycosylation and its impact on effector functions13
Integrated continuous biomanufacturing on pilot scale for acid‐sensitive monoclonal antibodies13
Bayesian Optimization in Bioprocess Engineering—Where Do We Stand Today?13
Cover Image, Volume 118, Number 12, December 202113
Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches13
High‐level production of wild‐type and oxidation‐resistant recombinant alpha‐1‐antitrypsin in glycoengineered CHO cells13
Designer probiotics: Opening the new horizon in diagnosis and prevention of human diseases13
Hierarchical dynamic regulation of Saccharomyces cerevisiae for enhanced lutein biosynthesis13
Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges13
A New Aerosol‐Based Photobioreactor for the Cultivation of Cyanobacteria on Luffa13
Predictive scaling of fiber‐based protein A capture chromatography using mechanistic modeling12
Biotechnology and Bioengineering: Volume 122, Number 1, January 202512
Physics‐informed neural networks for biopharmaceutical cultivation processes: Consideration of varying process parameter settings12
Impact of dual‐baculovirus infection on the Sf9 insect cell transcriptome during rAAV production using single‐cell RNA‐seq12
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Recent advances in biomedical applications of carbon and graphene quantum dots: A review12
Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium12
Technological advances in ocular trabecular meshwork in vitro models for glaucoma research12
Virus filtration: A review of current and future practices in bioprocessing12
Enhanced acetate utilization for value‐added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis12
Early dynamic changes in iPSC oxygen consumption rate predict future cardiomyocyte differentiation12
A fluidized‐bed‐riser adsorption system for continuous bioproduct recovery from crude feedstock12
Modeling bioaffinity‐based targeted delivery of antimicrobials to Escherichia coli biofilms using yeast microparticles. Part II: Parameter evaluation and validation12
2,6‐diaminopurine (Z)‐containing toehold probes improve genotyping sensitivity12
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Biotechnology and Bioengineering: Volume 120, Number 4, April 202312
Bioengineered 3D microfibrous‐matrix modulates osteopontin release from MSCs and facilitates the expansion of hematopoietic stem cells12
Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications12
Directed Evolution of an (R)‐Selective Transaminase Toward Higher Efficiency of Sitagliptin Analog Biosynthesis12
Insights into product and process related challenges of lentiviral vector bioprocessing12
Development of growth selection system and pocket engineering of d‐amino acid oxidase to enhance selective deamination activity toward d‐phosphinothricin12
Real‐time monitoring of biomass during Escherichia coli high‐cell‐density cultivations by in‐line photon density wave spectroscopy12
Modeling the Performance of an Anaerobic Moving Bed Biofilm Reactor12
Rapid optimization of processes for the integrated purification of biopharmaceuticals12
Robust platform for inline Raman monitoring and control of perfusion cell culture12
Systematic workflow for studying domain contributions of bispecific antibodies to selectivity in multimodal chromatography12
CFD‐based bioreactor model with proportional–integral–derivative controller functionality for dissolved oxygen and pH12
Electrospinning of stimuli‐responsive polymers for controlled drug delivery: pH‐ and temperature‐driven release12
Cover Image, Volume 120, Number 3, March 202312
Protein fouling during constant‐flux virus filtration: Mechanisms and modeling12
An integrated yeast‐based process for cis,cis‐muconic acid production12
Enrichment of adeno‐associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients12
A kinetic framework for modeling oleochemical biosynthesis in Escherichia coli11
Coevolving stability and activity of LsCR by a single point mutation and constructing neat substrate bioreaction system11
Sequential delivery of photosensitizers and checkpoint inhibitors by engineered bacteria for enhanced cancer photodynamic immunotherapy11
Revisiting mouse minute virus inactivation by high temperature short time treatment11
Expansion and differentiation of ex vivo cultured erythroblasts in scalable stirred bioreactors11
Engineering of the Lrp/AsnC‐type transcriptional regulator DecR as a genetically encoded biosensor for multilevel optimization of L‐cysteine biosynthesis pathway in Escherichia coli11
Process intensification for the production of yellow fever virus‐like particles as potential recombinant vaccine antigen11
“Toehold Switches; a foothold for Synthetic Biology”11
Combining descriptive and predictive modeling to systematically design depth filtration‐based harvest processes for biologics11
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Modeling large‐scale bioreactors with diffusion equations. Part I: Predicting axial dispersion coefficient and mixing times11
Technology transfer of a monitoring system to predict product concentration and purity of biopharmaceuticals in real‐time during chromatographic separation11
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