Biotechnology and Bioengineering

Papers
(The TQCC of Biotechnology and Bioengineering is 9. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2021-09-01 to 2025-09-01.)
ArticleCitations
122
Super7 passaging method to improve Chinese hamster ovary cell fed‐batch performance73
An engineered ultrahigh affinity bi‐paratopic uPAR targeting agent confers enhanced tumor targeting53
Optical discrimination of pathological red blood cells53
Back Cover Image, Volume 118, Number 11, November 202150
Harnessing metabolic plasticity in CHO cells for enhanced perfusion cultivation49
Continuous low pH viral inactivation: Operation and scaling strategy informs viral clearance study49
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Effects of biofilm heterogeneity on the apparent mechanical properties obtained by shear rheometry47
Evaluating potential of tissue‐engineered cryogels and chondrocyte derived exosomes in articular cartilage repair45
Computational fluid dynamics based digital twins of fixed bed bioreactors validate scaling principles for recombinant adeno‐associated virus gene therapy manufacturing43
Dual‐Stage Cross‐Flow Filtration: Integrated Capture and Purification of Virus‐Like Particles42
Constructing multi‐enzymatic cascade reactions for selective production of 6‐bromoindirubin from tryptophan in Escherichia coli42
Concentration‐dependent diffusion of unlabeled protein within an in vitro hyaluronic acid matrix42
Application and progress of 3D tumor models in breast cancer41
Iron in blood cells: Function, relation to disease, and potential for magnetic separation41
Dual‐targeting multifunctional hyaluronic acid ligand‐capped gold nanorods with enhanced endo‐lysosomal escape ability for synergetic photodynamic–photothermal therapy of cancer40
Biotechnology and Bioengineering: Volume 122, Number 5, May 202540
Application of gas diffusion electrodes in bioeconomy: An update40
Repressing expression of difficult‐to‐express recombinant proteins during the selection process increases productivity of CHO stable pools37
pH Threshold Impacts Chalcopyrite Bioleaching Dynamics for the Extreme Thermoacidophile Sulfurisphaera ohwakuensis36
RSEA: A Web Server for Pathway Enrichment Analysis of Metabolic Reaction Sets36
Current Advances and Future Prospects of Bulk and Microfluidic‐Enabled Electroporation Systems35
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Expression of filaments of theGeobacterextracellular cytochrome OmcS inShewanella oneidensis34
Concentration of clarified pool by single‐pass tangential flow filtration to improve productivity of protein A capture step: Impact of clarification strategies34
Heterologous Expression of Candida antarctica Lipase B in Aspergillus niger Using CRISPR/Cas9‐mediated Multi‐Gene Editing33
Double‐reporter‐guided targeted activation of the oxytetracycline silent gene cluster in Streptomyces rimosus M52733
Continuous polyhydroxybutyrate production from biogas in an innovative two‐stage bioreactor configuration33
Enhanced bioprocess control to advance the manufacture of mesenchymal stromal cell‐derived extracellular vesicles in stirred‐tank bioreactors33
High‐throughput screening of optimal process conditions using model predictive control33
Semiautomated design and soluble expression of a chimeric antigen TbpAB01 from Glaesserella parasuis32
Analyzing protein conjugation reactions for antibody‐drug conjugate synthesis using polarized excitation emission matrix spectroscopy32
Microbial production of odd‐chain fatty acids31
Development of generic metabolic Raman calibration models using solution titration in aqueous phase and data augmentation for in‐line cell culture analysis31
Experimental evolution reveals an effective avenue for d‐lactic acid production from glucose‐xylose mixtures via enhanced Glk activity and a cAMP‐independent CRP mutation30
From spores to fungal pellets: A new high‐throughput image analysis highlights the structural development of Aspergillus niger30
Kinetic characterization and modeling of sequentially entrapped enzymes in 3D‐printed PMMA microfluidic reactors for the synthesis of amorphadiene via the isopentenol utilization pathway28
A robust multinutrient kinetic model for enhanced lutein and biomass yields in mixotrophic microalgae cultivation: A step towards successful large‐scale productions28
Comparative genomic and transcriptomic analysis guides to further enhance the biosynthesis of erythromycin by an overproducer28
Engineering Bienzymatic Cascade for Efficient Biosynthesis of Citronellal and Citronellol27
Machine learning approaches to root cause analysis, characterization, and monitoring of subvisible particles in monoclonal antibody formulations27
Acoustic levitation as a tool for cell‐driven self‐organization of human cell spheroids during long‐term 3D culture26
Accelerating attribute‐focused process and product development through the development and deployment of autonomous process analytical technology platform system26
Fine and combinatorial regulation of key metabolic pathway for enhanced β‐alanine biosynthesis with non‐inducible Escherichia coli26
Erratum for “Constitutively active Rheb mutants [T23M] and [E40K] drive increased production and secretion of recombinant protein in Chinese hamster ovary cells” (Vol. 118, Issue 7, pp. 2422–2434)26
Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli25
Isothermal titration calorimetric assessment of lignin conversion by laccases25
Culture system for longitudinal monitoring of bone dynamics ex vivo25
Bioprinted dermis with human adipose tissue‐derived microvascular fragments promotes wound healing25
Reinforcement learning based temperature control of a fermentation bioreactor for ethanol production25
A New Modeling Approach for Predicting the Growth of Filamentous Algae in Outdoor Algae‐Based Wastewater Treatment Systems25
Biocatalytic role of cytochrome P450s to produce antibiotics: A review25
Characterization and designing of an SAM riboswitch to establish a high‐throughput screening platform for SAM overproduction in Saccharomyces cerevisiae24
Improved production of heme using metabolically engineered Escherichia coli24
Increased Mevalonate Production Using Engineered Citrate Synthase and Phosphofructokinase Variants of Escherichia coli24
IR780 loaded gelatin‐PEG coated gold core silica shell nanorods for cancer‐targeted photothermal/photodynamic therapy24
Optimization of physical microenvironment to maintain the quiescence of human induced pluripotent stem cell‐derived hepatic stellate cells24
Physiological Response of Penicillium chrysogenum to Mimicked Local and Global Perturbations of Substrate and Dissolved Oxygen Gradients at Industrial‐Scale24
Reviewing the process intensification landscape through the introduction of a novel, multitiered classification for downstream processing23
Commitment of human mesenchymal stromal cells towards ACL fibroblast differentiation upon rAAV‐mediated FGF‐2 and TGF‐β overexpression using pNaSS‐grafted PCL films23
Establishment of low‐cost production platforms of polyhydroxyalkanoate bioplastics from Halomonas cupida J923
Transferrin Purification, Biophysical Characterization, and Lung Biodistribution in Sickle Cell Disease Mice23
Biotechnology and Bioengineering: Volume 121, Number 2, February 202423
Engineering an Overflow‐Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production23
Enabling biocatalysis in high‐concentration organic cosolvent by enzyme gate engineering23
Marine biological macromolecules as matrix material for biosensor fabrication22
Streamlined Clarification and Capture Process for Monoclonal Antibodies Using Fluidized Bed Centrifugation and Multi‐Column Chromatography With Membrane Adsorbers22
Dynamic optimization of an integrated cultivation‐aggregation model for mAb production22
Metal cofactor regulation combined with rational genetic engineering of Schizochytrium sp. for high‐yield production of squalene22
On‐column capping of poly dT media‐tethered mRNA accomplishes high capping efficiency, enhanced mRNA recovery, and improved stability against RNase22
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Single Cell Protein Production From Ethanol: Model‐Based Bioreactor Operation at Industrial Scale22
Rapid spectrophotometric detection for optimized production of landomycins and characterization of their therapeutic potential22
Artificial induction of chromosome aneuploidy in CHO cells alters their function as host cells22
Influence of circadian rhythm on drug metabolism in 3D hepatic spheroids22
Recent Research on Chondrocyte Dedifferentiation and Insights for Regenerative Medicine21
Lactic acid bacteria as a tool for biovanillin production: A review21
Structural variability, implementational irregularities in mathematical modelling of polyhydroxyalkanoates (PHAs) production—A state‐of‐the‐art review21
Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via “flow‐through affinity chromatography” using peptide‐based adsorbents21
Enhancing real‐time cell culture monitoring: Automated Raman model optimization with Taguchi method21
Biotechnological production of polyphosphate from industrial wash water21
Multi‐cell‐line learning for the data‐driven construction of mechanistic metabolic models21
Pareto optimal metabolic engineering for the growth‐coupled overproduction of sustainable chemicals21
Manufacturing a chimpanzee adenovirus‐vectored SARS‐CoV‐2 vaccine to meet global needs21
Oxygen Consumption in Filamentous Pellets of Aspergillus niger: Microelectrode Measurements and Modeling21
Open‐Source Anaerobic Digestion Modeling Platform, Anaerobic Digestion Model No. 1 Fast (ADM1F)21
Techno‐Economic Analysis of Membrane‐Based Purification Platforms for AAV Vector Production20
Elucidating the effects of microbubble pinch‐off dynamics on mammalian cell viability20
Process monitoring framework for cross‐flow diafiltration‐based virus‐like particle disassembly: Tracing product properties and filtration performance20
Bringing a scalable adaptive hybrid modeling framework closer to industrial use: Application on a multiscale fungal fermentation20
Biotechnology and Bioengineering: Volume 122, Number 7, July 202520
PCR‐ and wash‐free detection of serum miRNA via signaling probe hybridization20
Bead to Bead Transfer, an Efficient Strategy to Scale‐up the Mesenchymal Stromal Cells‐Derived Secretome Bioproduction20
Covalent organic frameworks (COFs) as carrier for improved drug delivery and biosensing applications19
Metabolic analysis of the asparagine and glutamine dynamics in an industrial Chinese hamster ovary fed‐batch process19
Efficient biosynthesis of α‐aminoadipic acid via lysine catabolism in Escherichia coli19
Cover Image, Volume 121, Number 8, August 202419
Effect of protein fouling on filtrate flux and virus breakthrough behaviors during virus filtration process19
Substrate and nutrient manipulation during continuous cultivation of extremophilic algae, Galdieria spp. RTK 37.1, substantially impacts biomass productivity and composition19
Fabrication and in vitro evaluation of 3D printed porous silicate substituted calcium phosphate scaffolds for bone tissue engineering19
Development of a novel purification method for AAV vectors using tangential flow filtration19
Sulfur‐driven autotrophic denitrification of nitric oxide for efficient nitrous oxide recovery19
Increasing yield of in vitro transcription reaction with at‐line high pressure liquid chromatography monitoring19
Enhanced harvest performance predictability through advanced multivariate data analysis of mammalian cell culture particle size distribution18
Cole‐Cole modeling of real‐time capacitance data for estimation of cell physiological properties in recombinant Escherichia coli cultivation18
Effects of Air Temperature on Transient Expression of Influenza Hemagglutinin in Nicotiana benthamiana: Analysis of Transgene Transcription and Plant Stress Responses18
Model‐based control system design to manage process parameters in mammalian cell culture for biopharmaceutical manufacturing18
Continuous monitoring of IgG using immobilized fluorescent reporters18
Corrigendum to “High‐Throughput Investigation of Endothelial‐to‐Mesenchymal Transformation (EndMT) With Combinatorial Cellular Microarrays”18
Resistive Method for Bacterial Detection Employing a Silicon Nanowire Electrical Chip18
Efficient and precise control of gene expression in Geobacter sulfurreducens through new genetic elements and tools for pollutant conversion18
Automated calibration and in‐line measurement of product quality during therapeutic monoclonal antibody purification using Raman spectroscopy18
Engineering Corynebacterium glutamicum for synthesis of poly(3‐hydroxybutyrate) from lignocellulose biomass18
Platform development for high‐throughput optimization of perfusion processes: Part I: Implementation of cell bleeds in microwell plates17
Monitoring and control of the release of soluble O2 from H2O2 inside porous enzyme carrier for O2 supply to an immobilized d‐amino acid oxidase17
Validation and scalability of homemade polycaprolactone macrobeads grafted with thermo‐responsive poly(N‐isopropylacrylamide) for mesenchymal stem cell expansion and harvesting17
Effects of pyruvate on primary metabolism and product quality for a high‐density perfusion process17
Real‐Time Auto Controlling of Viable Cell Density in Perfusion Cultivation Aided by In‐Line Dielectric Spectroscopy With Segmented Adaptive PLS Model17
Impact of Hormone on Growth and GA3 Regulation of Anthocyanin Biosynthesis in Suspension‐Culture Cells of Cyclocarya paliurus17
Comparison of metabolic kinetics during high and low solids anaerobic digestion of fecal sludge17
One‐Pot Hetero‐Di‐C‐Glycosylation of the Natural Polyphenol Phloretin by a Single C‐Glycosyltransferase With Broad Sugar Substrate Specificity17
A Paradigm of Computer Vision and Deep Learning Empowers the Strain Screening and Bioprocess Detection17
Reconstruction and analysis of genome‐scale metabolic model for thermophilic fungus Myceliophthora thermophila17
Engineering dense tumor constructs via cellular contraction of extracellular matrix hydrogels17
H2O2 feeding enables LPMO‐assisted cellulose saccharification during simultaneous fermentative production of lactic acid16
Cast‐Molded Channelized Hydrogel Scaffolds With Stereolithography‐Printed Templates16
Engineering Yarrowia lipolytica for Efficient Production of Erythritol by Decoding and Harnessing Osmotic‐Stress‐Responsive Genetic Targets16
Biotechnology and Bioengineering: Volume 120, Number 10, October 202316
Genome‐scale metabolic modeling of Thraustochytrium sp. RT2316‐16: Effects of nutrients on metabolism16
Fungal assisted bio‐treatment of environmental pollutants with comprehensive emphasis on noxious heavy metals: Recent updates16
Biotechnology and Bioengineering: Volume 122, Number 9, September 202516
Engineering Escherichia coli asymmetry distribution‐based synthetic consortium for shikimate production16
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Biotechnology and Bioengineering: Volume 121, Number 11, November 202416
Deep hybrid modeling of a HEK293 process: Combining long short‐term memory networks with first principles equations16
A proteomics approach to decipher a sticky CHO situation16
Optimized Biosynthetic Pathway for Nonnatural Amino Acids: An Efficient Approach for L‐2‐Aminobutyric Acid Production16
Engineered yeast for efficient de novo synthesis of 7‐dehydrocholesterol16
Biotechnology and Bioengineering: Volume 120, Number 5, May 202316
Biointerface engineering through amalgamation of gene technology and site‐specific growth factor conjugation for efficient osteodifferentiation16
Design an Energy‐Conserving Pathway for Efficient Biosynthesis of 1,5‐Pentanediol and 5‐Amino‐1‐Pentanol15
A computational model for single cell Lamin‐A structural organization after microfluidic compression15
Enzyme‐functionalized alginate microparticles enable anaerobic culture under ambient oxygen15
Mesenchymal Stem Cells‐Derived Small Extracellular Vesicles and Their Validation as a Promising Treatment for Chondrosarcoma in a 3D Model in Vitro15
Identification of yttrium oxide‐specific peptides for future recycling of rare earth elements from electronic scrap15
Using MVDA with stoichiometric balances to optimize amino acid concentrations in chemically defined CHO cell culture medium for improved culture performance15
Structure‐function analysis of two closely related cutinases from Thermobifida cellulosilytica15
On‐chip magnetophoretic capture in a model of malaria‐infected red blood cells15
An adaptive soft‐sensor for advanced real‐time monitoring of an antibody‐drug conjugation reaction15
Titer improvement of milbemycins via coordinating metabolic competition and transcriptional co‐activation controlled by Streptomyces antibiotic regulatory protein family regulator in Strepto15
Perfusion Process Intensification for Lentivirus Production Using a Novel Scale‐Down Model15
Cover Image, Volume 121, Number 2, February 202415
Advanced cell technologies: Making protein, cell, and gene therapies a reality15
Modeling large‐scale bioreactors with diffusion equations. Part II: Characterizing substrate, oxygen, temperature, carbon dioxide, and pH profiles15
Interpretable‐AI‐Based Model Structural Transfer Learning to Accelerate Bioprocess Model Construction14
Manipulation of mRNA translation elongation influences the fragmentation of a biotherapeutic Fc‐fusion protein produced in CHO cells14
Plant extract‐based synthesis of metallic nanomaterials, their applications, and safety concerns14
Back Cover Image, Volume 120, Number 4, April 202314
Fibrin gel enhanced trilayer structure in cell‐cultured constructs14
Higher efficiency of vanadate iron in heterogeneous Fenton‐like systems to pretreat sugarcane bagasse and its enzymatic saccharification14
Role of carbon nanotubes (CNTs) in transgenic plant development14
Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturing14
Systems engineering Escherichia coli for efficient production p‐coumaric acid from glucose14
In silico and in vivo studies of the effect of surface curvature on the osteoconduction of porous scaffolds14
Cover Image, Volume 118, Number 12, December 202114
Sensitivity analysis and adaptive mutation strategy differential evolution algorithm for optimizing enzymes' turnover numbers in metabolic models14
Construction of a CRISPR‐Cas9‐Based Genetic Editing Tool for Serratia marcescens Using a Stationary Phase Promoter and Its Application in Putrescine Production14
Engineering an Escherichia coli based in vivo mRNA manufacturing platform14
Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stress14
Biosynthesis of allantoin in Escherichia coli via screening a highly effective urate oxidase14
Cover Image, Volume 119, Number 5, May 202214
Scalable manufacturing platform for the production of PEGylated heme albumin14
Reducing Structural Nonidentifiabilities in Upstream Bioprocess Models Using Profile‐Likelihood13
Insight of oral vaccines as an alternative approach to health and disease management: An innovative intuition and challenges13
Transfer learning Bayesian optimization for competitor DNA molecule design for use in diagnostic assays13
A New Aerosol‐Based Photobioreactor for the Cultivation of Cyanobacteria on Luffa13
High‐level production of wild‐type and oxidation‐resistant recombinant alpha‐1‐antitrypsin in glycoengineered CHO cells13
Designer probiotics: Opening the new horizon in diagnosis and prevention of human diseases13
Integrated continuous biomanufacturing on pilot scale for acid‐sensitive monoclonal antibodies13
The interplay of protein engineering and glycoengineering to fine‐tune antibody glycosylation and its impact on effector functions13
Understanding the effects of aerodynamic and hydrodynamic shear forces on Pseudomonas aeruginosa biofilm growth13
Are K‐strategists yield‐strategists in disguise? An example from autotrophic nitrogen removal13
CFD‐based bioreactor model with proportional–integral–derivative controller functionality for dissolved oxygen and pH13
Hierarchical dynamic regulation of Saccharomyces cerevisiae for enhanced lutein biosynthesis13
Assessment of Anticancer Effects of Aloe vera on 3D Liver Tumor Spheroids in a Microfluidic Platform13
Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches13
Quorum sensing unveils the sludge floccule‐assisted stabilization of aerobic granules in granule‐dominated sequencing batch reactors13
Recent advances in biomedical applications of carbon and graphene quantum dots: A review13
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A multiscale modeling method for therapeutic antibodies in ion exchange chromatography13
Adjustment of the main biosynthesis modules to enhance the production of l‐homoserine in Escherichia coli W311013
Meso‐ and thermophilic posttreatment of press water coming from a thermophilic municipal solid waste digester13
N2‐fixation can sustain wastewater treatment performance of photogranules under nitrogen‐limiting conditions13
Binding stability of peptides to Co–Cr–Mo alloy affects proliferation and differentiation of osteoblast13
Bayesian Optimization in Bioprocess Engineering—Where Do We Stand Today?13
Technological advances in ocular trabecular meshwork in vitro models for glaucoma research12
Coevolving stability and activity of LsCR by a single point mutation and constructing neat substrate bioreaction system12
Early dynamic changes in iPSC oxygen consumption rate predict future cardiomyocyte differentiation12
2,6‐diaminopurine (Z)‐containing toehold probes improve genotyping sensitivity12
Modeling the Performance of an Anaerobic Moving Bed Biofilm Reactor12
Construction of ajmalicine and sanguinarine de novo biosynthetic pathways using stable integration sites in yeast12
Physics‐informed neural networks for biopharmaceutical cultivation processes: Consideration of varying process parameter settings12
Cover Image, Volume 120, Number 3, March 202312
Systematic workflow for studying domain contributions of bispecific antibodies to selectivity in multimodal chromatography12
Challenges and opportunities in downstream separation processes for mesenchymal stromal cells cultured in microcarrier‐based stirred suspension bioreactors12
Biotechnology and Bioengineering: Volume 120, Number 4, April 202312
Inside Front Cover Image, Volume 122, Number 8, August 202512
Dual cytoplasmic‐peroxisomal engineering for high‐yield production of sesquiterpene α‐humulene in Yarrowia lipolytica12
Modeling bioaffinity‐based targeted delivery of antimicrobials to Escherichia coli biofilms using yeast microparticles. Part II: Parameter evaluation and validation12
Real‐time monitoring of biomass during Escherichia coli high‐cell‐density cultivations by in‐line photon density wave spectroscopy12
Enhanced acetate utilization for value‐added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis12
An integrated yeast‐based process for cis,cis‐muconic acid production12
Biotechnology and Bioengineering: Volume 122, Number 1, January 202512
Impact of dual‐baculovirus infection on the Sf9 insect cell transcriptome during rAAV production using single‐cell RNA‐seq12
Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications12
Electrospinning of stimuli‐responsive polymers for controlled drug delivery: pH‐ and temperature‐driven release11
Protein fouling during constant‐flux virus filtration: Mechanisms and modeling11
Bioengineered 3D microfibrous‐matrix modulates osteopontin release from MSCs and facilitates the expansion of hematopoietic stem cells11
Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium11
Biotechnology and Bioengineering: Volume 119, Number 11, November 202211
Computational framework to understand the clinical stages of COVID‐19 and visualization of time course for various treatment strategies11
Design and development of opto‐electrochemical biosensing devices for diagnosing chronic kidney disease11
The Missing Link: Connecting Cultivation Conditions and Refolding Performance via Inclusion Body Biophysical Properties11
Predictive scaling of fiber‐based protein A capture chromatography using mechanistic modeling11
A fluidized‐bed‐riser adsorption system for continuous bioproduct recovery from crude feedstock11
Fabrication of oxygen‐carrying microparticles functionalized with liver ECM‐proteins to improve phenotypic three‐dimensional in vitro liver assembly, function, and responses11
Systematic review: External carbon source for biological denitrification for wastewater11
Transcriptional biomarker discovery toward building a load stress reporting system for engineered Escherichia coli strains11
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Enrichment of adeno‐associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients11
Virus filtration: A review of current and future practices in bioprocessing11
Unsupervised Machine Learning‐Based Process Analytical Tools for Near Real‐Time Cell Morphology Analysis During CAR‐T Cell Manufacturing11
Modular RNAi Pathway Engineering Enhances Plasmid Copy Number Control in Yeast Bioproduction System11
Robust platform for inline Raman monitoring and control of perfusion cell culture11
Insights into product and process related challenges of lentiviral vector bioprocessing11
Modeling Analysis of Oxygen Transfer Efficiency in Rest Cell Catalysis for Extra High‐Titer Xylonic Acid Bioproduction11
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Engineering of the Lrp/AsnC‐type transcriptional regulator DecR as a genetically encoded biosensor for multilevel optimization of L‐cysteine biosynthesis pathway in Escherichia coli11
Development of growth selection system and pocket engineering of d‐amino acid oxidase to enhance selective deamination activity toward d‐phosphinothricin11
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Directed Evolution of an (R)‐Selective Transaminase Toward Higher Efficiency of Sitagliptin Analog Biosynthesis11
Cover Image, Volume 121, Number 10, October 202410
In situ biological particle analyzer based on digital inline holography10
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Sensors and chemometrics in downstream processing10
Cover Image, Volume 119, Number 9, September 202210
Identification and characterization of CHO host‐cell proteins in monoclonal antibody bioprocessing10
Identification of optimal flow rate for culture media, cell density, and oxygen toward maximization of virus production in a fed‐batch baculovirus‐insect cell system10
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