Biotechnology and Bioengineering

Papers
(The TQCC of Biotechnology and Bioengineering is 8. The table below lists those papers that are above that threshold based on CrossRef citation counts [max. 250 papers]. The publications cover those that have been published in the past four years, i.e., from 2020-11-01 to 2024-11-01.)
ArticleCitations
Scale‐up economics for cultured meat117
The role of extracellular DNA in the formation, architecture, stability, and treatment of bacterial biofilms78
“High‐risk” host cell proteins (HCPs): A multi‐company collaborative view76
Biogenesis and mechanisms of microRNA‐mediated gene regulation70
Overview on immobilization of enzymes on synthetic polymeric nanofibers fabricated by electrospinning52
Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green alga Chlorella sorokiniana for biomass production50
A review of manufacturing capabilities of cell spheroid generation technologies and future development49
A genome‐scale metabolic network model and machine learning predict amino acid concentrations in Chinese Hamster Ovary cell cultures43
A common framework for integrated and continuous biomanufacturing42
Modeling of photosynthesis and respiration rate for microalgae–bacteria consortia41
End‐to‐end continuous bioprocessing: Impact on facility design, cost of goods, and cost of development for monoclonal antibodies41
Advances in mapping the epigenetic modifications of 5‐methylcytosine (5mC), N6‐methyladenine (6mA), and N4‐methylcytosine (4mC)39
A CRISPR‐Cas12a‐derived biosensor enabling portable personal glucose meter readout for quantitative detection of SARS‐CoV‐239
Manufacturing a chimpanzee adenovirus‐vectored SARS‐CoV‐2 vaccine to meet global needs39
Production of β‐carotene in Saccharomyces cerevisiae through altering yeast lipid metabolism39
Pilot‐scale demonstration of an end‐to‐end integrated and continuous biomanufacturing process36
Autofluorescence of blood and its application in biomedical and clinical research35
Construction of ajmalicine and sanguinarine de novo biosynthetic pathways using stable integration sites in yeast35
Extraction, isolation, purification, derivatization, bioactivity, structure–activity relationship, and application of polysaccharides from White jellyfungus35
Flux redistribution of central carbon metabolism for efficient production of l‐tryptophan in Escherichia coli34
Effect of alginate matrix engineered to mimic the pancreatic microenvironment on encapsulated islet function34
3D bioprinting and microscale organization of vascularized tissue constructs using collagen‐based bioink33
Outer membrane vesicles (OMVs) enabled bio‐applications: A critical review33
Functional expression of eukaryotic cytochrome P450s in yeast32
Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum32
Production of trimeric SARS‐CoV‐2 spike protein by CHO cells for serological COVID‐19 testing32
Dual cytoplasmic‐peroxisomal engineering for high‐yield production of sesquiterpene α‐humulene in Yarrowia lipolytica32
The usage of composite nanomaterials in biomedical engineering applications32
End‐to‐end collaboration to transform biopharmaceutical development and manufacturing32
Maximizing mRNA vaccine production with Bayesian optimization31
Miniaturized electrochemical biosensor based on whole‐cell for heavy metal ions detection in water31
A transfer learning approach for predictive modeling of bioprocesses using small data31
Microbial fatty acid transport proteins and their biotechnological potential31
Production of high‐quality SARS‐CoV‐2 antigens: Impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance30
Assessment of quality attributes for adeno‐associated viral vectors30
Scaffolds obtained from decellularized human extrahepatic bile ducts support organoids to establish functional biliary tissue in a dish29
Kinetic and hybrid modeling for yeast astaxanthin production under uncertainty29
Evaluating potential of tissue‐engineered cryogels and chondrocyte derived exosomes in articular cartilage repair29
A review of synthesis of esters with aromatic, emulsifying, and lubricant properties by biotransformation using lipases28
Plasticized poly(vinylalcohol) and poly(vinylpyrrolidone) based patches with tunable mechanical properties for cardiac tissue engineering applications28
Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions28
New horizons in developing cell lysis methods: A review26
A CRISPR/Cas9 eraser strategy for contamination‐free PCR end‐point detection26
Integrated continuous biomanufacturing on pilot scale for acid‐sensitive monoclonal antibodies25
Semirational engineering of an aldo–keto reductase KmAKR for overcoming trade‐offs between catalytic activity and thermostability25
Microfluidics for microalgal biotechnology25
Process development and optimization of continuous capture with three‐column periodic counter‐current chromatography25
Strategies and challenges with the microbial conversion of methanol to high‐value chemicals25
Vascularization of iNSC spheroid in a 3D spheroid‐on‐a‐chip platform enhances neural maturation25
Technological tools and strategies for culturing human gut microbiota in engineered in vitro models25
Improving an intensified and integrated continuous bioprocess platform for biologics manufacturing25
Transformation of lignocellulose to starch‐like carbohydrates by organic acid‐catalyzed pretreatment and biological detoxification24
A generalized machine‐learning aided method for targeted identification of industrial enzymes from metagenome: A xylanase temperature dependence case study24
Evaluating the effects of microparticle addition on mycelial morphology, natural yellow pigments productivity, and key genes regulation in submerged fermentation of Monascus purpureus24
Systematic assessment of process analytical technologies for biologics24
Knowledge transfer across cell lines using hybrid Gaussian process models with entity embedding vectors24
Biologically rapid synthesized silver nanoparticles from aqueous Eucalyptus camaldulensis leaf extract: Effects on hyphal growth, hydrolytic enzymes, and biofilm formation in Candida albican24
Directed evolution of unspecific peroxygenase in organic solvents24
Halophilic archaea and their potential to generate renewable fuels and chemicals23
A high‐throughput and machine learning resistance monitoring system to determine the point of resistance for Escherichia coli with tetracycline: Combining UV‐visible spectrophotometry with prin23
Rapid and multiplex detection of nosocomial pathogens on a phage‐based magnetoresistive lab‐on‐chip platform23
Metabolic engineering of oleaginous yeast Rhodotorula toruloides for overproduction of triacetic acid lactone23
Recent trends using natural polymeric nanofibers as supports for enzyme immobilization and catalysis23
Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organoids reveals faster commitment under dynamic conditions23
3D printed human organoids: High throughput system for drug screening and testing in current COVID‐19 pandemic23
Cyclic l‐lactide synthesis from lignocellulose biomass by biorefining with complete inhibitor removal and highly simultaneous sugars assimilation23
Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications23
Enhancing tryptophan production by balancing precursors in Escherichia coli23
Recent advances in computational methods for biosensor design22
Hydrogen peroxide‐producing electrochemical bandage controlled by a wearable potentiostat for treatment of wound infections22
A new model to analyze the temperature effect on the microalgae performance at large scale raceway reactors22
Universal law for diffusive mass transport through mycelial networks21
Reduced type‐A carbohydrate‐binding module interactions to cellulose I leads to improved endocellulase activity21
Stretch‐driven microfluidic chip for nucleic acid detection21
Strategies to control therapeutic antibody glycosylation during bioprocessing: Synthesis and separation21
Scale‐up of an intensified bioprocess for the expansion of bovine adipose‐derived stem cells (bASCs) in stirred tank bioreactors21
Development of a dynamic in vitro stretch model of the alveolar interface with aerosol delivery21
Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid21
Engineering Clostridium cellulovorans for highly selective n‐butanol production from cellulose in consolidated bioprocessing20
Extracellular vesicles facilitate large‐scale dynamic exchange of proteins and RNA among cultured Chinese hamster ovary and human cells20
Technical‐scale biophotovoltaics for long‐term photo‐current generation from Synechocystis sp. PCC680320
Rapid cGMP manufacturing of COVID‐19 monoclonal antibody using stable CHO cell pools20
Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via “flow‐through affinity chromatography” using peptide‐based adsorbents20
3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering20
Biodegradable and biocompatible graphene‐based scaffolds for functional neural tissue engineering: A strategy approach using dental pulp stem cells and biomaterials20
Increasing yield of in vitro transcription reaction with at‐line high pressure liquid chromatography monitoring20
Virus filtration: A review of current and future practices in bioprocessing20
Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin19
Scaffold‐free biofabrication of adipocyte structures with magnetic levitation19
Engineered yeast for efficient de novo synthesis of 7‐dehydrocholesterol19
Bioreactor production of rVSV‐based vectors in Vero cell suspension cultures19
IR780 loaded gelatin‐PEG coated gold core silica shell nanorods for cancer‐targeted photothermal/photodynamic therapy19
Miniature auto‐perfusion bioreactor system with spiral microfluidic cell retention device19
Development and application of a cultivation platform for mammalian suspension cell lines with single‐cell resolution19
Rapid RNase inhibitor production to enable low‐cost, on‐demand cell‐free protein synthesis biosensor use in human body fluids19
Marine biological macromolecules as matrix material for biosensor fabrication19
Gram‐scale production of the sesquiterpene α‐humulene with Cupriavidus necator19
Accelerating and de‐risking CMC development with transposon‐derived manufacturing cell lines19
Critical challenges and advances in recombinant adeno‐associated virus (rAAV) biomanufacturing19
Comparison of UV‐ and Raman‐based monitoring of the Protein A load phase and evaluation of data fusion by PLS models and CNNs19
Characterizing the effect of substrate stiffness on the extravasation potential of breast cancer cells using a 3D microfluidic model19
Sortase A: A chemoenzymatic approach for the labeling of cell surfaces19
Indirect regulation of temperature in raceway reactors by optimal management of culture depth19
PAMAM G4.5 dendrimers for targeted delivery of ferulic acid and paclitaxel to overcome P‐glycoprotein‐mediated multidrug resistance18
Scalable, methanol‐free manufacturing of the SARS‐CoV‐2 receptor‐binding domain in engineered Komagataella phaffii18
White paper on high‐throughput process development for integrated continuous biomanufacturing18
Effects of benzyl viologen on increasing NADH availability, acetate assimilation, and butyric acid production by Clostridium tyrobutyricum18
Microparticles enhance the formation of seven major classes of natural products in native and metabolically engineered actinobacteria through accelerated morphological development18
Enhancing biosynthesis of 2'‐Fucosyllactose in Escherichia coli through engineering lactose operon for lactose transport and α ‐1,2‐Fucosyltransferase for solubility18
Temperature and oxygen level determine N2O respiration activities of heterotrophic N2O‐reducing bacteria: Biokinetic study18
Plant extract‐based synthesis of metallic nanomaterials, their applications, and safety concerns18
Characterization of HIV‐1 virus‐like particles and determination of Gag stoichiometry for different production platforms18
An integrated continuous downstream process with real‐time control: A case study with periodic countercurrent chromatography and continuous virus inactivation18
Recent progress on small molecular temperature‐sensitive fluorescent probes18
Angiogenic and osteogenic effects of flavonoids in bone regeneration17
Synergising biomass growth kinetics and transport mechanisms to simulate light/dark cycle effects on photo‐production systems17
A CHO stable pool production platform for rapid clinical development of trimeric SARS‐CoV‐2 spike subunit vaccine antigens17
The effects of flux on the clearance of minute virus of mice during constant flux virus filtration17
Systematic review: External carbon source for biological denitrification for wastewater17
Process design of a fully integrated continuous biopharmaceutical process using economic and ecological impact assessment17
On‐chip determination of tissue‐specific metastatic potential of breast cancer cells17
IL‐10 lentivirus‐laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury17
Assessing the influence of perfusion on cardiac microtissue maturation: A heart‐on‐chip platform embedding peristaltic pump capabilities17
Advanced nano biosensors for rapid detection of zoonotic bacteria17
Conferring thermotolerant phenotype to wild‐type Yarrowia lipolytica improves cell growth and erythritol production17
Cell‐derived and enzyme‐based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial17
An integrated biomimetic array chip for establishment of collagen‐based 3D primary human hepatocyte model for prediction of clinical drug‐induced liver injury17
Recent advances in enzymatic biosensors for point‐of‐care detection of biomolecules17
Modeling apoptosis resistance in CHO cells with CRISPR‐mediated knockouts of Bak1, Bax, and Bok17
How to measure diffusion coefficients in biofilms: A critical analysis17
Resource allocation explains lactic acid production in mixed‐culture anaerobic fermentations16
Control of surge tanks for continuous manufacturing of monoclonal antibodies16
Multiple strategies for metabolic engineering of Escherichia coli for efficient production of glycolate16
High‐ and low‐molecular‐weight chitosan act as adjuvants during single‐dose influenza A virus protein vaccination through distinct mechanisms16
Engineered pro‐peptide enhances the catalytic activity of keratinase to improve the conversion ability of feather waste16
Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches16
Application of metabolic modeling for targeted optimization of high seeding density processes16
Structure‐function analysis of two closely related cutinases from Thermobifida cellulosilytica16
Macroscopic modeling of bioreactors for recombinant protein producingPichia pastorisin defined medium16
Long‐term biofouling formation mediated by extracellular proteins in Nannochloropsis gaditana microalga cultures at different medium N/P ratios16
Improved production of heme using metabolically engineered Escherichia coli16
Rapid and low‐cost sampling for detection of airborne SARS‐CoV‐2 in dehumidifier condensate16
Foam fractionation methods in aerobic fermentation processes16
An effective computational‐screening strategy for simultaneously improving both catalytic activity and thermostability of α‐l‐rhamnosidase16
Antibody disulfide bond reduction and recovery during biopharmaceutical process development—A review16
A high‐throughput expression and screening platform for applications‐driven PETase engineering16
Biocatalytic synthesis of dihydroxy fatty acids as lipid mediators from polyunsaturated fatty acids by double dioxygenation of the microbial 12S‐lipoxygenase16
Anchor‐IMPACT: A standardized microfluidic platform for high‐throughput antiangiogenic drug screening16
Design, fabrication, and characterization of a multimodal reconfigurable bioreactor for bone tissue engineering15
Reducing phenotypic instabilities of a microbial population during continuous cultivation based on cell switching dynamics15
Magnetic levitation assisted biofabrication, culture, and manipulation of 3D cellular structures using a ring magnet based setup15
Multi‐information source Bayesian optimization of culture media for cellular agriculture15
Dispersion of sulfur creates a valuable new growth medium formulation that enables earlier sulfur oxidation in relation to iron oxidation in Acidithiobacillus ferrooxidans cultures15
Introduction of glycine synthase enables uptake of exogenous formate and strongly impacts the metabolism in Clostridium pasteurianum15
A computational learning paradigm to targeted discovery of biocatalysts from metagenomic data: A case study of lipase identification15
Multiorgan‐on‐a‐chip for realization of gut‐skin axis15
A scalable bubble‐free membrane aerator for biosurfactant production15
Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications15
A high cell density perfusion process for Modified Vaccinia virus Ankara production: Process integration with inline DNA digestion and cost analysis15
The design basis for the integrated and continuous biomanufacturing framework15
Metabolic analysis of the asparagine and glutamine dynamics in an industrial Chinese hamster ovary fed‐batch process15
Continuous integrated manufacturing for biopharmaceuticals: A new paradigm or an empty promise?15
Identification two key residues at the intersection of domains of a thioether monooxygenase for improving its sulfoxidation performance15
Optimizing recombineering in Corynebacterium glutamicum14
Pseudomonas putida KT2440 endures temporary oxygen limitations14
Combined gene and environmental engineering offers a synergetic strategy to enhance r‐protein production in Chinese hamster ovary cells14
Tracing production instability in a clonally derived CHO cell line using single‐cell transcriptomics14
Fungal assisted bio‐treatment of environmental pollutants with comprehensive emphasis on noxious heavy metals: Recent updates14
All‐in‐one microfluidic design to integrate vascularized tumor spheroid into high‐throughput platform14
Cloning and characterization of a panel of mitochondrial targeting sequences for compartmentalization engineering in Saccharomyces cerevisiae14
Promoter engineering‐mediated Tuning of esterase and transaminase expression for the chemoenzymatic synthesis of sitagliptin phosphate at the kilogram‐scale14
Magnetoelastic sensors for real‐time tracking of cell growth14
SDF‐1α gene‐activated collagen scaffold drives functional differentiation of human Schwann cells for wound healing applications14
Strengthening the CAR‐T cell therapeutic application using CRISPR/Cas9 technology14
A new perfusion mode of culture for WJ‐MSCs expansion in a stirred and online monitored bioreactor14
A peptide‐based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging14
Nanoengineered biomimetic hydrogels: A major advancement to fabricate 3D‐printed constructs for regenerative medicine14
In‐silico media optimization for continuous cultures using genome scale metabolic networks: The case of CHO‐K114
Development of an E. coli strain for cell‐free ADC manufacturing14
Identification of preferred multimodal ligand‐binding regions on IgG1 FC using nuclear magnetic resonance and molecular dynamics simulations13
Biotechnological production of cyclic dinucleotides—Challenges and opportunities13
Preparation of lignin nanoparticles via ultra‐fast microwave‐assisted fractionation of lignocellulose using ternary deep eutectic solvents13
Targeted CHO cell engineering approaches can reduce HCP‐related enzymatic degradation and improve mAb product quality13
Process intensification for the production of yellow fever virus‐like particles as potential recombinant vaccine antigen13
Combined local delivery of tacrolimus and stem cells in hydrogel for enhancing peripheral nerve regeneration13
A hydrogel–fiber–hydrogel composite scaffold based on silk fibroin with the dual‐delivery of oxygen and quercetin13
A multiscale modeling method for therapeutic antibodies in ion exchange chromatography13
Nonviral siRNA delivery systems for pancreatic cancer therapy13
Electrical stimulation waveform‐dependent osteogenesis on PVDF/BaTiO3 composite using a customized and programmable cell stimulator13
Characterization and implications of host‐cell protein aggregates in biopharmaceutical processing13
Enabling biocatalysis in high‐concentration organic cosolvent by enzyme gate engineering13
Modeling the impact of amino acid substitution in a monoclonal antibody on cation exchange chromatography13
Cashew nut shell oil as a potential feedstock for biodiesel production: An overview13
Nonviral gene delivery to T cells with Lipofectamine LTX13
Hierarchical dynamic regulation of Saccharomyces cerevisiae for enhanced lutein biosynthesis13
Hyperosmolality in CHO culture: Effects on cellular behavior and morphology13
Techno‐economic modeling and assessment of cultivated meat: Impact of production bioreactor scale13
Integration of metabolic pathway manipulation and promoter engineering for the fine‐tuned biosynthesis of malic acid in Bacillus coagulans13
Carboxylates and alcohols production in an autotrophic hydrogen‐based membrane biofilm reactor13
Overview of the advances in plant microbial fuel cell technology for sustainable energy recovery from rhizodeposition13
Production of recombinant butyrylcholinesterase from transgenic rice cell suspension cultures in a pilot‐scale bioreactor13
High‐throughput screening of optimal process conditions using model predictive control13
Multi‐omics profiling of a CHO cell culture system unravels the effect of culture pH on cell growth, antibody titer, and product quality13
Comparison of partial least square, artificial neural network, and support vector regressions for real‐time monitoring of CHO cell culture processes using in situ near‐infrared spectroscopy12
Pressure‐dependent fouling behavior during sterile filtration of mRNA‐containing lipid nanoparticles12
Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells12
Engineering Corynebacterium glutamicum for synthesis of poly(3‐hydroxybutyrate) from lignocellulose biomass12
Simple plant‐based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor‐targeted drug delivery and bioimaging in cancer therapy12
Design of synthetic promoters for controlled expression of therapeutic genes in retinal pigment epithelial cells12
Microbial production of odd‐chain fatty acids12
Outdoor scale‐up of Leptolyngbya sp.: Effect of light intensity and inoculum volume on photoinhibition and ‐oxidation12
Rapid at‐line early cell death quantification using capacitance spectroscopy12
De novo sequence redesign of a functional Ras‐binding domain globally inverted the surface charge distribution and led to extreme thermostability12
Downstream processing for influenza vaccines and candidates: An update12
Selective production of retinol by engineered Saccharomyces cerevisiae through the expression of retinol dehydrogenase12
Sustainable liquid supports for laccase immobilization and reuse: Degradation of dyes in aqueous biphasic systems12
Metabolic engineering of Escherichia coli for efficient osmotic stress‐free production of compatible solute hydroxyectoine12
Fabrication and in vitro evaluation of 3D printed porous silicate substituted calcium phosphate scaffolds for bone tissue engineering12
Bioengineering of a CLiP‐derived tubular biliary‐duct‐like structure for bile transport in vitro12
From spores to fungal pellets: A new high‐throughput image analysis highlights the structural development of Aspergillus niger12
An integrated yeast‐based process for cis,cis‐muconic acid production12
Extracellular electron transfer via multiple electron shuttles in waterborneAeromonas hydrophilafor bioreduction of pollutants12
Device for respiration activity measurement enables the determination of oxygen transfer rates of microbial cultures in shaken 96‐deepwell microtiter plates12
An engineered cellobiohydrolase I for sustainable degradation of lignocellulosic biomass12
Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells12
Engineered acetoacetate‐inducible whole‐cell biosensors based on the AtoSC two‐component system12
Production of afucosylated antibodies in CHO cells by coexpression of an anti‐FUT8 intrabody12
Development of a microbe domestication pod (MD Pod) for in situ cultivation of micro‐encapsulated marine bacteria12
Imaging flow cytometry data analysis using convolutional neural network for quantitative investigation of phagocytosis12
Data‐driven prediction models for forecasting multistep ahead profiles of mammalian cell culture toward bioprocess digital twins12
Electrospinning of stimuli‐responsive polymers for controlled drug delivery: pH‐ and temperature‐driven release11
Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturing11
Advances in bioreactor control for production of biotherapeutic products11
Dynamic regulation of membrane integrity to enhance l‐malate stress tolerance in Candida glabrata11
Sulfur‐driven autotrophic denitrification of nitric oxide for efficient nitrous oxide recovery11
Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut‐microbial studies11
Application of gas diffusion electrodes in bioeconomy: An update11
In silico and in vivo studies of the effect of surface curvature on the osteoconduction of porous scaffolds11
Evaluation of tissue integration of injectable, cell‐laden hydrogels of cocultures of mesenchymal stem cells and articular chondrocytes with an ex vivo cartilage explant model11
Carboxymethyl cellulose–alginate interpenetrating hydroxy ethyl methacrylate crosslinked polyvinyl alcohol reinforced hybrid hydrogel templates with improved biological performance for cardiac tissue 11
Metabolic engineering of HEK293 cells to improve transient transfection and cell budding of HIV‐1 virus‐like particles11
Design of transfections: Implementation of design of experiments for cell transfection fine tuning11
A label‐free and signal‐amplifiable assay method for colorimetric detection of carcinoembryonic antigen11
Metabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum11
Process intensification to produce a difficult‐to‐express therapeutic enzyme by high cell density perfusion or enhanced fed‐batch11
Partitioning metabolism between growth and product synthesis for coordinated production of wax esters in Acinetobacter baylyi ADP111
Mechano‐regulation of vascular network formation without branches in 3D bioprinted cell‐laden hydrogel constructs11
Hybrid modeling in bioprocess dynamics: Structural variabilities, implementation strategies, and practical challenges11
Uncoupling growth and succinic acid production in an industrial Saccharomyces cerevisiae strain11
Morphological landscapes from high content imaging reveal cytokine priming strategies that enhance mesenchymal stromal cell immunosuppression11
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