Biotechnology and Bioengineering

Article	Citations
Biotechnology and Bioengineering: Volume 121, Number 1, January 2024	117
Electrospinning of stimuli‐responsive polymers for controlled drug delivery: pH‐ and temperature‐driven release	84
Outside Back Cover Image, Volume 118, Number 7, July 2021	76
Cover Image, Volume 120, Number 3, March 2023	70
Concentration‐dependent diffusion of unlabeled protein within an in vitro hyaluronic acid matrix	55
Biotechnology and Bioengineering: Volume 120, Number 8, August 2023	52
Selection of synthetic proteins to modulate the human frataxin function	44
Engineering and characterization of carbohydrate‐binding modules for imaging cellulose fibrils biosynthesis in plant protoplasts	43
In‐silico media optimization for continuous cultures using genome scale metabolic networks: The case of CHO‐K1	43
Combined multivariate statistical and flux balance analyses uncover media bottlenecks to the growth and productivity of Chinese hamster ovary cell cultures	42
Skeletal muscle‐adipose cocultured tissue fabricated using cell‐laden microfibers and a hydrogel sheet	39
Construction of ajmalicine and sanguinarine de novo biosynthetic pathways using stable integration sites in yeast	39
Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum	39
Knowledge transfer across cell lines using hybrid Gaussian process models with entity embedding vectors	38
Design and development of opto‐electrochemical biosensing devices for diagnosing chronic kidney disease	35
Magnetoelastic sensors for real‐time tracking of cell growth	35
Nonviral gene delivery to T cells with Lipofectamine LTX	35
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A hydrogel–fiber–hydrogel composite scaffold based on silk fibroin with the dual‐delivery of oxygen and quercetin	33
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Biotechnology and Bioengineering: Volume 119, Number 2, February 2022	33
Investigating and modeling the effect of light intensity on Rhodopseudomonas palustris growth	32
Attenuation of glutamine synthetase selection marker improves product titer and reduces glutamine overflow in Chinese hamster ovary cells	32
Differential N‐ and O‐glycosylation signatures of HIV‐1 Gag virus‐like particles and coproduced extracellular vesicles	32
Impact of dual‐baculovirus infection on the Sf9 insect cell transcriptome during rAAV production using single‐cell RNA‐seq	31

Bioinformatics‐driven discovery of novel Clostridioides difficile lysins and experimental comparison with highly active benchmarks	31
Biotechnology and Bioengineering: Volume 120, Number 4, April 2023	30
Cover Image, Volume 120, Number 4, April 2023	30
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Mechanobiological stimulation in organ‐on‐a‐chip systems reduces hepatic drug metabolic capacity in favor of regenerative specialization	29
2,6‐diaminopurine (Z)‐containing toehold probes improve genotyping sensitivity	28
Harnessing metabolic plasticity in CHO cells for enhanced perfusion cultivation	28
Challenges and opportunities in downstream separation processes for mesenchymal stromal cells cultured in microcarrier‐based stirred suspension bioreactors	26
Modeling of copy number variability in Pichia pastoris	26
Medium composition overturns the widely accepted sulfate‐dependent repression of desulfurization phenotype in Rhodococcus qingshengii IGTS8	26
Back Cover Image, Volume 118, Number 11, November 2021	26
Modeling bioaffinity‐based targeted delivery of antimicrobials to Escherichia coli biofilms using yeast microparticles. Part II: Parameter evaluation and validation	26
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Cover Image, Volume 118, Number 8, August 2021	25
Biotechnology and Bioengineering: Volume 118, Number 12, December 2021	25
Biotechnology and Bioengineering: Volume 118, Number 11, November 2021	25
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Systems‐level approaches for understanding and engineering of the oleaginous cell factory Yarrowia lipolytica	24
Epigenomic features revealed by ATAC‐seq impact transgene expression in CHO cells	24
Improving stability of human three dimensional skin equivalents using plasma surface treatment	24
Cover Image, Volume 119, Number 8, August 2022	24
Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost‐derived consortium	24
Continuous capture scale down model: A comparison of a continuous and a discrete approach	23
Hydrogen online monitoring based on thermal conductivity for anaerobic microorganisms	23
Double‐reporter‐guided targeted activation of the oxytetracycline silent gene cluster in Streptomyces rimosus M527	23
Biotechnology and Bioengineering: Volume 119, Number 10, October 2022	23
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Factors affecting product association as a mechanism of host‐cell protein persistence in bioprocessing	23
Metabolic and process engineering for microbial production of protocatechuate with Corynebacterium glutamicum	23
Insights into product and process related challenges of lentiviral vector bioprocessing	22
A review on the upstream production and downstream purification of mannosylerythritol lipids	21
CALU‐3 lung cells three‐dimensionally assembled onto CellFate® matrix present angiotensin‐converting enzyme‐2 activity	21
Contributions of Chinese hamster ovary cell derived extracellular vesicles and other cellular materials to hollow fiber filter fouling during perfusion manufacturing of monoclonal antibodies	21
High‐throughput CRISPR‐mediated 3D enrichment platform for functional interrogation of chemotherapeutic resistance	21
Conferring thermotolerant phenotype to wild‐type Yarrowia lipolytica improves cell growth and erythritol production	21
Technological advances in ocular trabecular meshwork in vitro models for glaucoma research	21
Advances in early diagnosis of cervical cancer based on biosensors	21
Data‐driven modeling of heterogeneous viscoelastic biofilms	20
SLAM‐seq reveals early transcriptomic response mechanisms upon glutamine deprivation in Chinese hamster ovary cells	20
Process analytical technology for on‐line monitoring of quality attributes during single‐use ultrafiltration/diafiltration	20
Biotechnology and Bioengineering: Volume 119, Number 12, December 2022	20
Microfluidic cultivation and analysis of productive biofilms	20
Bioengineered 3D microfibrous‐matrix modulates osteopontin release from MSCs and facilitates the expansion of hematopoietic stem cells	20
Development of a rapid polarized total synchronous fluorescence spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth	20
Fabrication of oxygen‐carrying microparticles functionalized with liver ECM‐proteins to improve phenotypic three‐dimensional in vitro liver assembly, function, and responses	20
Dual‐targeting multifunctional hyaluronic acid ligand‐capped gold nanorods with enhanced endo‐lysosomal escape ability for synergetic photodynamic–photothermal therapy of cancer	20
Differential effects on natural killer cell production by membrane‐bound cytokine stimulations	20
Enzyme purification and sustained enzyme activity for pharmaceutical biocatalysis by fusion with phase‐separating intrinsically disordered protein	19
Engineering of Rieske dioxygenase variants with improved cis‐dihydroxylation activity for benzoates	19

Three‐dimensional co‐culture model employing silica nonwoven fabrics to enhance cell‐to‐cell communication of paracrine signaling between hepatocytes and fibroblasts	19
Super7 passaging method to improve Chinese hamster ovary cell fed‐batch performance	19
Screening microbiota for effects on host tissues	19
Non‐industrial production of therapeutic lentiviral vectors: How to provide vectors to academic CAR‐T	19
An engineered ultrahigh affinity bi‐paratopic uPAR targeting agent confers enhanced tumor targeting	19
Optical discrimination of pathological red blood cells	19
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A new methodology for a rapid and high‐throughput comparison of molecular profiles and biological activity of phytoextracts	19
Adaptive laboratory evolution in a novel parallel shaken pH‐auxostat	19
Improvement of TaC9‐ABE mediated correction of human SMN2 gene	19
Application of gas diffusion electrodes in bioeconomy: An update	18
Peptide ligands for the universal purification of exosomes by affinity chromatography	18
Rational design of disulfide bonds to increase thermostability of Rhodococcus opacus catechol 1,2 dioxygenase	18
hMSCs in contact with DMSO for cryopreservation: Experiments and modeling of osmotic injury and cytotoxic effect	18
Outer membrane vesicles (OMVs) enabled bio‐applications: A critical review	18
Predictive modeling for cell culture in commercial manufacturing of biotherapeutics	18
Advancements in nanobody generation: Integrating conventional, in silico, and machine learning approaches	18
Use of a 3D inkjet‐printed model to access dust particle toxicology in the human alveolar barrier	18
Optimizing the production of dsRNA biocontrols in microbial systems using multiple transcriptional terminators	18
Ecotoxicity characterization assisted performance assessment of electro‐bioremediation reactors for nitrate and arsenite elimination	18
Real‐time detection of mAb aggregates in an integrated downstream process	17
Producing high value unsaturated fatty acid by whole‐cell catalysis using microalga: A case study with Tribonema minus	17
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Efficient and safe small RNA delivery to macrophage using peptide‐based nanocomplex	17
Decellularized adipose tissue matrix‐coated and simvastatin‐loaded hydroxyapatite microspheres for bone regeneration	17
Machine learning‐based model predictive controller design for cell culture processes	17
Rapid optimization of processes for the integrated purification of biopharmaceuticals	17
Robust platform for inline Raman monitoring and control of perfusion cell culture	17
Modeling a propionate‐oxidizing syntrophic coculture using thermodynamic principles	17
Passage number of cancer cell lines: Importance, intricacies, and way‐forward	17
Combinatorial InVitroFlow‐assisted mutagenesis (CombIMut) yields a 41‐fold improved CelA2 cellulase	17
Constructing multi‐enzymatic cascade reactions for selective production of 6‐bromoindirubin from tryptophan in Escherichia coli	17
Nanopore sequencing improves construction of customized CRISPR‐based gene activation libraries	17
Biotechnology and Bioengineering: Volume 121, Number 6, June 2024	17
Quorum sensing‐based metabolic engineering of the precursor supply in Streptomyces coelicolor to improve heterologous production of neoaureothin	16
Impact of microcarrier concentration on mesenchymal stem cell growth and death: Experiments and modeling	16
Kinetic and functional analysis of abundant microRNAs in extracellular vesicles from normal and stressed cultures of Chinese hamster ovary cells	16
Nanoengineered biomimetic hydrogels: A major advancement to fabricate 3D‐printed constructs for regenerative medicine	16
Protein fouling during constant‐flux virus filtration: Mechanisms and modeling	16
Recent advancements in enzyme‐incorporated nanomaterials: Synthesis, mechanistic formation, and applications	16
The yeastGemMap: A process diagram to assist yeast systems‐metabolic studies	16
Whole‐cell biosynthesis of cytarabine by an unnecessary protein‐reduced Escherichia coli that coexpresses purine and uracil phosphorylase	16
Best practices on critical reagent characterization, qualification, and life cycle management for HCP immunoassays	16
Coevolutionary analysis reveals a distal amino acid residue pair affecting the catalytic activity of GH5 processive endoglucanase from Bacillus subtilis BS‐5	16
Fast‐growing phototrophic microorganisms and the productivity of phototrophic cultures	16
Technical‐scale biophotovoltaics for long‐term photo‐current generation from Synechocystis sp. PCC6803	16
Rapid at‐line early cell death quantification using capacitance spectroscopy	16
Optimization of continuous TAG production by Nannochloropsis gaditana in solar‐nitrogen‐limited culture	16
3D printed human organoids: High throughput system for drug screening and testing in current COVID‐19 pandemic	16
Engineered pro‐peptide enhances the catalytic activity of keratinase to improve the conversion ability of feather waste	16
Designing bacterial consortia for the complete biodegradation of insensitive munitions compounds in waste streams	15
Tracing production instability in a clonally derived CHO cell line using single‐cell transcriptomics	15
Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids	15
Performance of a new family of modular, bed‐supported, chromatography devices	15
Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium	15
A combined experimental and computational framework to evaluate the behavior of therapeutic cells for peripheral nerve regeneration	15
Iron in blood cells: Function, relation to disease, and potential for magnetic separation	15
Transcriptomics and cell painting analysis reveals molecular and morphological features associated with fed‐batch production performance in CHO recombinant clones	15
Optimized operation of a controlled stirred tank reactor system for the production of mesenchymal stromal cells and their extracellular vesicles	15
A fluidized‐bed‐riser adsorption system for continuous bioproduct recovery from crude feedstock	15
Pushing the boundaries of phosphorylase cascade reaction for cellobiose production I: Kinetic model development	15
Improvement of protein production by engineering a novel antiapoptotic baculovirus vector to suppress the expression ofSf‐caspase‐1andTn‐caspase‐1	15
Continuous countercurrent chromatographic twin‐column purification of oligonucleotides: The role of the displacement effect	15
Accelerating and de‐risking CMC development with transposon‐derived manufacturing cell lines	15
Conversion and synthesis of chemicals catalyzed by fungal cytochrome P450 monooxygenases: A review	15
Continuous low pH viral inactivation: Operation and scaling strategy informs viral clearance study	15
Data‐driven prediction models for forecasting multistep ahead profiles of mammalian cell culture toward bioprocess digital twins	14
Improving the genetic stability of bacterial growth control for long‐term bioproduction	14
Elucidating solvent effects on lipase‐catalyzed peroxyacid synthesis through activity‐based kinetics and molecular dynamics	14
A label‐free and signal‐amplifiable assay method for colorimetric detection of carcinoembryonic antigen	14
Systematic review: External carbon source for biological denitrification for wastewater	14
Concentration of clarified pool by single‐pass tangential flow filtration to improve productivity of protein A capture step: Impact of clarification strategies	14
Development of growth selection system and pocket engineering of d‐amino acid oxidase to enhance selective deamination activity toward d‐phosphinothricin	14
Metabolomic characterization of monoclonal antibody‐producing Chinese hamster lung (CHL)‐YN cells in glucose‐controlled serum‐free fed‐batch operation	14
Cell culture product quality attribute prediction using convolutional neural networks and Raman spectroscopy	14
Combining genetic engineering and bioprocess concepts for improved phenylpropanoid production	14
Dual cytoplasmic‐peroxisomal engineering for high‐yield production of sesquiterpene α‐humulene in Yarrowia lipolytica	14
Mechanistic modeling of in vitro transcription incorporating effects of magnesium pyrophosphate crystallization	14
Computational fluid dynamics based digital twins of fixed bed bioreactors validate scaling principles for recombinant adeno‐associated virus gene therapy manufacturing	14
Spatial organization of pathway enzymes via self‐assembly to improve 2′‐fucosyllactose biosynthesis in engineered Escherichia coli	14

Coevolving stability and activity of LsCR by a single point mutation and constructing neat substrate bioreaction system	13
Systematic workflow for studying domain contributions of bispecific antibodies to selectivity in multimodal chromatography	13
3D printing to construct in vitro multicellular models of melanoma	13
Role, relevance, and possibilities of in vitro fermentation models in human dietary, and gut‐microbial studies	13
Improving an intensified and integrated continuous bioprocess platform for biologics manufacturing	13
Lactoyl leucine and isoleucine are bioavailable alternatives for canonical amino acids in cell culture media	13
High‐throughput screening of optimal process conditions using model predictive control	13
Time‐dependent sorption behavior of lentiviral vectors during anion‐exchange chromatography	13
Impacts of hydrodynamic conditions and microscale surface roughness on the critical shear stress to develop and thickness of early‐stage Pseudomonas putida biofilms	13
Predictive scaling of fiber‐based protein A capture chromatography using mechanistic modeling	13
A genome‐scale nutrient minimization forecast algorithm for controlling essential amino acid levels in CHO cell cultures	13
Rational design of short‐chain dehydrogenase/reductase for enantio‐complementary synthesis of chiral 1,2‐diols by successive hydroxymethylation and reduction of aldehydes	13
Cashew nut shell oil as a potential feedstock for biodiesel production: An overview	13
Dual‐Stage Cross‐Flow Filtration: Integrated Capture and Purification of Virus‐Like Particles	13
Early dynamic changes in iPSC oxygen consumption rate predict future cardiomyocyte differentiation	13
Rational design of transaminases based on comparative analysis of catalytically active and distance‐free modes of the high‐energy intermediate state	13
Virus filtration: A review of current and future practices in bioprocessing	13
Microfluidics for microalgal biotechnology	13
Autofluorescence of blood and its application in biomedical and clinical research	13
Effects of biofilm heterogeneity on the apparent mechanical properties obtained by shear rheometry	13
An integrated yeast‐based process for cis,cis‐muconic acid production	13
Enhanced acetate utilization for value‐added chemicals production in Yarrowia lipolytica by integration of metabolic engineering and microbial electrosynthesis	13
Effect of chromosomal integration on catalytic performance of a multi‐component P450 system in Escherichia coli	12
Chromosome recombination and modification by LoxP‐mediated evolution in Vibrio natriegens using CRISPR‐associated transposases	12
Clinical applicability of optogenetic gene regulation	12
Repressing expression of difficult‐to‐express recombinant proteins during the selection process increases productivity of CHO stable pools	12
Evaluating potential of tissue‐engineered cryogels and chondrocyte derived exosomes in articular cartilage repair	12
Overview on immobilization of enzymes on synthetic polymeric nanofibers fabricated by electrospinning	12
Genome‐wide CRISPR/Cas9 knockout screening to mitigate cell growth inhibition induced by histone deacetylase inhibitors in recombinant CHO cells	12
High‐Efficiency Genome Editing in Naturally Isolated Aeromonas hydrophila and Edwardsiella Piscicida Using the CRISPR‐Cas9 System	12
Enhanced bioprocess control to advance the manufacture of mesenchymal stromal cell‐derived extracellular vesicles in stirred‐tank bioreactors	12
End‐to‐end continuous bioprocessing: Impact on facility design, cost of goods, and cost of development for monoclonal antibodies	12
Dispersion of sulfur creates a valuable new growth medium formulation that enables earlier sulfur oxidation in relation to iron oxidation in Acidithiobacillus ferrooxidans cultures	12
Enrichment of adeno‐associated virus serotype 5 full capsids by anion exchange chromatography with dual salt elution gradients	12
A novel approach to residence time distribution characterization in a mAb continuous process	12
A new T‐antigen negative HEK293 cell line with improved AAV productivity	12
Real‐time monitoring of biomass during Escherichia coli high‐cell‐density cultivations by in‐line photon density wave spectroscopy	12
Erratum for “Advances in mapping the epigenetic modifications of 5‐methylcytosine (5mC), N6‐methyladenine (6mA), and N4‐methylcytosine (4mC)” (Vol. 118, Issue 11, pp. 4204–4216)	12
Realization of digital twin for dynamic control toward sample variation of ion exchange chromatography in antibody separation	12
Miniature auto‐perfusion bioreactor system with spiral microfluidic cell retention device	12
Online monitoring and control of upstream cell culture process using 1D and 2D‐LC with SegFlow interface	12
Cationic polymer precipitation for enhanced impurity removal in downstream processing	12
Physiological suitability of sulfate‐reducing granules for the development of bioconcrete	12
Improving the activity and thermostability of GH2 β‐glucuronidases via domain reassembly	12
Morphological landscapes from high content imaging reveal cytokine priming strategies that enhance mesenchymal stromal cell immunosuppression	12
Simple, rapidly electroassembled thiolated PEG‐based sensor interfaces enable rapid interrogation of antibody titer and glycosylation	12
Ultrahigh‐cell‐density heterotrophic cultivation of the unicellular green alga Chlorella sorokiniana for biomass production	12
Metabolic engineering of betacyanin in vegetables for anti‐inflammatory therapy	12
Exploring the potential of engineering polygalacturonase‐inhibiting protein as an ecological, friendly, and nontoxic pest control agent	12
End‐to‐end collaboration to transform biopharmaceutical development and manufacturing	12
Leloir glycosyltransferases enabled to flow synthesis: Continuous production of the natural C‐glycoside nothofagin	12
Application and progress of 3D tumor models in breast cancer	11
Online monitoring of methane transfer rates unveils nitrogen fixation dynamics in Methylococcus capsulatus	11
Expression of filaments of theGeobacterextracellular cytochrome OmcS inShewanella oneidensis	11
3D Printed GelMA/CHIMA Cross‐Linked Network Hydrogel for Angiogenesis	11
Alterations in Protein Phosphorylation and Arginine Biosynthesis Metabolism Confer β‐Phenylethanol Tolerance in Saccharomyces cerevisiae	11
Sensors and chemometrics in downstream processing	11
Optimization of physical microenvironment to maintain the quiescence of human induced pluripotent stem cell‐derived hepatic stellate cells	11
pH Threshold Impacts Chalcopyrite Bioleaching Dynamics for the Extreme Thermoacidophile Sulfurisphaera ohwakuensis	11
Physics‐informed neural networks for biopharmaceutical cultivation processes: Consideration of varying process parameter settings	11
Erratum for “Rewiring central carbon metabolism for tyrosol and salidroside production inSaccharomyces cerevisiae” (Vol. 117, Issue 8, pp. 2410–2419)	11
Impact of anodophilic biofilm bioelectroactivity on the denitrification behavior of air‐cathode microbial fuel cells	11
Machine Learning‐Powered Optimization of a CHO Cell Cultivation Process	11
Modeling the Performance of an Anaerobic Moving Bed Biofilm Reactor	11
Scale‐up analysis of geometrically dissimilar single‐use bioreactors	11
A microfluidic approach for studying microcolonization of Escherichia coli O157:H7 on leaf trichome‐mimicking surfaces under fluid shear stress	11
Biotechnology and Bioengineering: Volume 122, Number 2, February 2025	11
Biotechnology and Bioengineering: Volume 122, Number 1, January 2025	11
Monitoring of the Biotechnological Production of Dihydroxyacetone Using a Low‐Field 1H NMR Spectrometer	11
High‐Efficiency CRISPR‐Cas9 Genome Editing Unveils Biofilm Insights and Enhances Antimicrobial Activity in Bacillus velezensis FZB42	11
Sub‐second heat inactivation of coronavirus using a betacoronavirus model	11
Machine learning approaches to root cause analysis, characterization, and monitoring of subvisible particles in monoclonal antibody formulations	11
Biotechnology and Bioengineering: Volume 119, Number 1, January 2022	11
Substantial gradient mitigation in simulated large‐scale bioreactors by optimally placed multiple feed points	11
Metabolic engineering of oleaginous yeast Rhodotorula toruloides for overproduction of triacetic acid lactone	10
Ofloxacin‐loaded HMPB NPs for Klebsiella pneumoniae eradication in the surgical wound with the combination of PTT	10
Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing	10
Scale‐up of an intensified bioprocess for the expansion of bovine adipose‐derived stem cells (bASCs) in stirred tank bioreactors	10
Promoter engineering‐mediated Tuning of esterase and transaminase expression for the chemoenzymatic synthesis of sitagliptin phosphate at the kilogram‐scale	10
Engineered modular microphysiological models of the human airway clearance phenomena	10
Engineering active lysostaphin variants that incorporate noncanonical amino acids and characterizing the effects of site‐specific PEGylation	10
Biotechnology and Bioengineering: Volume 120, Number 6, June 2023	10
Extraction, isolation, purification, derivatization, bioactivity, structure–activity relationship, and application of polysaccharides from White jellyfungus	10
Mechanistic modeling of viral particle production	10
Current state of implementation of in silico tools in the biopharmaceutical industry—Proceedings of the 5th modeling workshop	10
Vaccine process technology—A decade of progress	10
A multiauxotrophic CHO cell line for the rapid isolation of producers of diverse or high levels of recombinant proteins	10
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Engineering Escherichia coli native metabolism for efficient biosynthesis of orotate	10
Integrating high cell density cultures with adapted laboratory evolution for improved Gag‐HA virus‐like particles production in stable insect cell lines	10
A kinetic framework for modeling oleochemical biosynthesis in Escherichia coli	10
Combining descriptive and predictive modeling to systematically design depth filtration‐based harvest processes for biologics	10
Characterization and designing of an SAM riboswitch to establish a high‐throughput screening platform for SAM overproduction in Saccharomyces cerevisiae	10
Scale‐up issues for commercial depth filters in bioprocessing	10
Biotechnology and Bioengineering: Volume 120, Number 1, January 2023	10